首页 > 最新文献

Hellenic Plant Protection Journal最新文献

英文 中文
Repellent effect of Gardenia jasminoides ethanol extracted oil on Blattella germanica and Monomorium pharaonis 栀子乙醇提取油对德国小蠊和法老王的驱避作用
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.2478/hppj-2023-0007
T. A. Wagan, C. Hamada, W. Cai, H. Hongxia, G. S. Solangi, R.H. Lyu
Summary The study examined the repellency of Gardenia jasminoides ethanol-extracted oil against the German cockroach, Blattella germanica, and the pharaoh ant, Monomorium pharaonis, which are serious pests in areas of public health hygiene. For the repellency tests, 31.4 μg of the oil was applied per cm2 on one half of filter paper discs (9 or 15 cm diameter for the ant and cockroach, respectively), whereas the other half was treated as control (DMSO + Tween). Repellency effects were observed 1, 2, 3 and 4 h after the insect release. The oil showed high repellency against all life stages of cockroaches and worker ants. The maximum repellency was observed for the cockroach adults (81.7 ± 3.1%) followed by the fourth, third and second nymphal stages (76.7 ± 4.2, 75.0 ± 3.4, and 56.7 ± 8.4%, respectively), after 1h exposure. The repellence effect was strong against worker ants (78.3 ± 4.8%) after 1 h exposure. The repellence effect can last at least four hours for both species. Analysis of Gardenia oil with Gas chromatography-mass spectrometry identified 14 major chemical components.
摘要本研究考察了栀子乙醇提取油对德国小蠊和法老蚁法老蚁的驱避作用,这两种害虫是公共卫生领域的严重害虫。对于排斥性测试,每cm2将31.4μg油涂抹在一半滤纸盘上(蚂蚁和蟑螂的直径分别为9或15 cm),而另一半作为对照(DMSO+Tween)。在昆虫释放后1、2、3和4小时观察到排斥作用。这种油对蟑螂和工蚁的所有生命阶段都表现出很高的排斥性。蟑螂成虫的排斥力最大(81.7±3.1%),然后是暴露1h后的第四、第三和第二若虫期(分别为76.7±4.2、75.0±3.4和56.7±8.4%)。暴露1h后对工蚁具有较强的排斥作用(78.3±4.8%)。对这两个物种来说,排斥作用至少可以持续四个小时。用气相色谱-质谱法对栀子油进行分析,鉴定出14种主要化学成分。
{"title":"Repellent effect of Gardenia jasminoides ethanol extracted oil on Blattella germanica and Monomorium pharaonis","authors":"T. A. Wagan, C. Hamada, W. Cai, H. Hongxia, G. S. Solangi, R.H. Lyu","doi":"10.2478/hppj-2023-0007","DOIUrl":"https://doi.org/10.2478/hppj-2023-0007","url":null,"abstract":"Summary The study examined the repellency of Gardenia jasminoides ethanol-extracted oil against the German cockroach, Blattella germanica, and the pharaoh ant, Monomorium pharaonis, which are serious pests in areas of public health hygiene. For the repellency tests, 31.4 μg of the oil was applied per cm2 on one half of filter paper discs (9 or 15 cm diameter for the ant and cockroach, respectively), whereas the other half was treated as control (DMSO + Tween). Repellency effects were observed 1, 2, 3 and 4 h after the insect release. The oil showed high repellency against all life stages of cockroaches and worker ants. The maximum repellency was observed for the cockroach adults (81.7 ± 3.1%) followed by the fourth, third and second nymphal stages (76.7 ± 4.2, 75.0 ± 3.4, and 56.7 ± 8.4%, respectively), after 1h exposure. The repellence effect was strong against worker ants (78.3 ± 4.8%) after 1 h exposure. The repellence effect can last at least four hours for both species. Analysis of Gardenia oil with Gas chromatography-mass spectrometry identified 14 major chemical components.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42308033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Behavioral and histopathological changes of Clarias gariepinus as a predatory fish against Culex pipiens larvae following exposure to sublethal concentration of quinclorac and bensulfuron-methyl based herbicide 在暴露于亚致死浓度的喹氯rac和苄磺隆甲基除草剂后,作为一种捕食性鱼类的加里平Clarias gariepinus对库蚊幼虫的行为和组织病理学变化
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.2478/hppj-2023-0009
I. Mohamed, M. Fathy, A. Farghal, S. Temerak, sary Abd El-ghaffar, Shaimaa K. Idriss
Summary Clarias gariepinus is one of the widespread culturable freshwater fish species in Africa, which is prevalent in various natural and human-made aquatic habitats including rice-fish system. This fish species displays predation potential on the aquatic stages of mosquitoes. Bensulfuron-methyl and quinclorac are herbicide active substances that have been extensively applied in rice culture in Egypt and other countries worldwide. This study assessed the adverse effects of sublethal concentration of a commercial herbicide formulation containing quinclorac and bensulfuron-methyl on the predation potential of C. gariepinus female and male predatory fish on Culex pipiens mosquito larvae. Also, stomach and intestine histopathology of the treated fish was investigated. The exposure of C. gariepinus to sublethal concentration of quinclorac and bensulfuron-methyl based herbicide produced detrimental effects on prey consumption and histopathological changes in the stomach and intestine of the fish. The mosquito consumption by the treated female and male fish decreased significantly compared to the untreated fish of both sexes. The histological changes in the intestines were hyperplasia of the intestinal epithelium and goblet cells; edema of lamina propria and broad intestinal villi, and distortion in intestinal villi in comparison to control. The stomach histopathology changes were necrosis and sloughing of mucosal epithelium with severe damage of sub-mucosa. Thus, the tested herbicide at sublethal concentration on C. gariepinus decreased the prey consumption on mosquito larvae and caused histopathological alterations in the fish that may impair its digestive physiology. These findings suggest a threat of the tested herbicide to C. gariepinus survival and potential as a native successful biocontrol agent against Cx. pipiens larvae.
加里扁尾鱼是非洲广泛可养殖的淡水鱼类之一,广泛分布在各种自然和人类水生栖息地,包括水稻鱼类系统。这种鱼类在蚊子的水生阶段表现出捕食的潜力。甲基苄磺隆和喹氯胺是一种除草剂活性物质,在埃及和世界其他国家的水稻栽培中得到了广泛应用。本研究评估了亚致死浓度的含有喹氯rac和甲基苄嘧磺隆的商业除草剂制剂对加里平线虫雌性和雄性捕食性鱼类捕食库蚊幼虫的潜在影响。此外,还对处理后的鱼的胃和肠组织病理学进行了研究。加里埃皮努斯暴露于亚致死浓度的喹氯胺酸和苄磺隆甲基除草剂对猎物的消耗以及鱼类胃肠道的组织病理学变化产生了不利影响。与未经处理的两性鱼类相比,经处理的雌性和雄性鱼类的蚊子消耗量显著减少。肠组织学改变为肠上皮和杯状细胞增生;与对照组相比,固有层和宽肠绒毛水肿以及肠绒毛扭曲。胃组织病理学改变为黏膜上皮坏死、脱落,亚黏膜损伤严重。因此,对C.gariepinus进行亚致死浓度测试的除草剂减少了蚊子幼虫的猎物消耗,并导致鱼类的组织病理学改变,这可能会损害其消化生理。这些发现表明,经测试的除草剂对加里皮锥虫的生存构成了威胁,并有可能成为一种成功的本地生物防治剂来防治库蚊幼虫。
{"title":"Behavioral and histopathological changes of Clarias gariepinus as a predatory fish against Culex pipiens larvae following exposure to sublethal concentration of quinclorac and bensulfuron-methyl based herbicide","authors":"I. Mohamed, M. Fathy, A. Farghal, S. Temerak, sary Abd El-ghaffar, Shaimaa K. Idriss","doi":"10.2478/hppj-2023-0009","DOIUrl":"https://doi.org/10.2478/hppj-2023-0009","url":null,"abstract":"Summary Clarias gariepinus is one of the widespread culturable freshwater fish species in Africa, which is prevalent in various natural and human-made aquatic habitats including rice-fish system. This fish species displays predation potential on the aquatic stages of mosquitoes. Bensulfuron-methyl and quinclorac are herbicide active substances that have been extensively applied in rice culture in Egypt and other countries worldwide. This study assessed the adverse effects of sublethal concentration of a commercial herbicide formulation containing quinclorac and bensulfuron-methyl on the predation potential of C. gariepinus female and male predatory fish on Culex pipiens mosquito larvae. Also, stomach and intestine histopathology of the treated fish was investigated. The exposure of C. gariepinus to sublethal concentration of quinclorac and bensulfuron-methyl based herbicide produced detrimental effects on prey consumption and histopathological changes in the stomach and intestine of the fish. The mosquito consumption by the treated female and male fish decreased significantly compared to the untreated fish of both sexes. The histological changes in the intestines were hyperplasia of the intestinal epithelium and goblet cells; edema of lamina propria and broad intestinal villi, and distortion in intestinal villi in comparison to control. The stomach histopathology changes were necrosis and sloughing of mucosal epithelium with severe damage of sub-mucosa. Thus, the tested herbicide at sublethal concentration on C. gariepinus decreased the prey consumption on mosquito larvae and caused histopathological alterations in the fish that may impair its digestive physiology. These findings suggest a threat of the tested herbicide to C. gariepinus survival and potential as a native successful biocontrol agent against Cx. pipiens larvae.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49517469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of exogenous abscisic acid on antioxidant system of salt tolerant and salt sensitive cotton cultivars 外源脱落酸对耐盐和盐敏感棉花抗氧化系统的影响
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.2478/hppj-2023-0006
K. Kuldoshova, A. Akhunov, N. Khashimova, J. Ziyavitdinov
Summary Salinity is one of the most imperative global problems that affect crop productivity on a large scale. Salinity impairs plant growth and development by imposing various stresses. Therefore it is vital to decode those stress factors and identify possible solutions to improve agriculture productivity. However, the adaptive mechanisms under saline conditions of glycophytes have not been studied. The present study was undertaken to determine the effects of exogenous abscisic acid (ABA) on salinity tolerance in cotton plants. Some patterns of resistance development were revealed on the seedlings of two cotton cultivars, a salt-tolerant (Gulistan) and a salt-sensitive one (C-4727). Moreover, the antioxidant potentials of these cultivars were compared. The activities of ascorbate peroxidase (APX), super-oxide dismutase (SOD), as well as the quantity of endogenous ABA, malondialdehyde (MDA), and free proline (Pro) were determined in control and post treatment. Our results demonstrate significant differences between the salt- tolerant and sensitive cotton seedlings in response to saline stress, i.e., high levels of Pro and endogenous ABA, but lower MDA concentrations, and higher activity of APX and SOD for the salt-tolerant cultivar, Gulistan, as compared to the salt stress-sensitive cultivar C-4727.
盐度是大规模影响作物生产力的最紧迫的全球问题之一。盐度通过施加各种压力而损害植物的生长和发育。因此,解读这些压力因素并确定提高农业生产力的可能解决方案至关重要。然而,糖生植物在生理盐水条件下的适应机制尚未得到研究。本研究旨在测定外源脱落酸(ABA)对棉花耐盐性的影响。耐盐品种Gulistan和耐盐品种C-4727的幼苗表现出一定的抗性发育模式。此外,还比较了这些品种的抗氧化能力。测定了处理前后抗坏血酸过氧化物酶(APX)、超氧化物歧化酶(SOD)的活性,以及内源ABA、丙二醛(MDA)和游离脯氨酸(Pro)的含量。我们的结果表明,与盐胁迫敏感品种C-4727相比,耐盐和敏感棉花幼苗对盐胁迫的反应存在显著差异,即耐盐品种Gulistan的Pro和内源ABA水平较高,但MDA浓度较低,APX和SOD活性较高。
{"title":"Effects of exogenous abscisic acid on antioxidant system of salt tolerant and salt sensitive cotton cultivars","authors":"K. Kuldoshova, A. Akhunov, N. Khashimova, J. Ziyavitdinov","doi":"10.2478/hppj-2023-0006","DOIUrl":"https://doi.org/10.2478/hppj-2023-0006","url":null,"abstract":"Summary Salinity is one of the most imperative global problems that affect crop productivity on a large scale. Salinity impairs plant growth and development by imposing various stresses. Therefore it is vital to decode those stress factors and identify possible solutions to improve agriculture productivity. However, the adaptive mechanisms under saline conditions of glycophytes have not been studied. The present study was undertaken to determine the effects of exogenous abscisic acid (ABA) on salinity tolerance in cotton plants. Some patterns of resistance development were revealed on the seedlings of two cotton cultivars, a salt-tolerant (Gulistan) and a salt-sensitive one (C-4727). Moreover, the antioxidant potentials of these cultivars were compared. The activities of ascorbate peroxidase (APX), super-oxide dismutase (SOD), as well as the quantity of endogenous ABA, malondialdehyde (MDA), and free proline (Pro) were determined in control and post treatment. Our results demonstrate significant differences between the salt- tolerant and sensitive cotton seedlings in response to saline stress, i.e., high levels of Pro and endogenous ABA, but lower MDA concentrations, and higher activity of APX and SOD for the salt-tolerant cultivar, Gulistan, as compared to the salt stress-sensitive cultivar C-4727.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45255761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of nano-silica extracted from two different plant sources on survival and development of Phthorimaea opercullela (Zeller) larvae 从两种不同植物来源提取的纳米二氧化硅对小蠊幼虫生存和发育的影响
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.2478/hppj-2023-0008
I. Idris, M. Naddaf, H. Harmalani, R. Alshater, R. Alsafadi
Summary The study examined the effect of nano-silica extracted from two different plant sources on the survival and development of the potato tuber moth, Phthorimaea opercullela. The silica powder was derived from two different agricultural byproducts, olive stones and corncobs. Characterization by X-ray diffraction revealed that the extracted powder has an amorphous silica phase. The nitrogen adsorption-desorption measurements revealed that both extracted and treated silica have mesoporous structure, with a specific surface area of around 300 m2/g and 270 m2/g for the silica derived from olive stones and corncobs, respectively. The silica nanoparticles (SiO2 NPs) prepared from the silica derived from olive stοnes showed higher larvae mortality, pupae weight, and larval and pupal developmental time, compared to the silica derived from corncombs. The results show that the nano-silica derived from agriculture byproducts can be as effective as the synthetic insecticide (deltamethrin) utilized in control of the potato tuber moth, with lower environmental impact in terms of preventing pesticide residue accumulation. In addition, the efficiency of SiO2 NPs applications depends on the source of the silica nanoparticles and the applied concentration to achieve the optimum results for the pest control.
摘要研究了两种不同植物源提取的纳米二氧化硅对马铃薯块茎蛾(Phthorimaea opercullela)存活和发育的影响。二氧化硅粉末来源于两种不同的农业副产品,橄榄石和玉米芯。x射线衍射表征表明,提取的粉末具有无定形的二氧化硅相。氮吸附-解吸测量表明,提取的二氧化硅和处理的二氧化硅都具有介孔结构,橄榄石和玉米芯的二氧化硅的比表面积分别约为300 m2/g和270 m2/g。硅纳米颗粒从硅(二氧化硅NPs)准备来自橄榄圣οnes显示更高的幼虫死亡率、蛹重、幼虫和蛹的发育时间,比硅来自corncombs。结果表明,农副产物纳米二氧化硅防治马铃薯块茎蛾的效果与人工合成杀虫剂(溴氰菊酯)相当,但在防止农药残留积累方面对环境的影响较小。此外,二氧化硅纳米颗粒的应用效率取决于二氧化硅纳米颗粒的来源和应用浓度,以达到最佳的害虫防治效果。
{"title":"Effect of nano-silica extracted from two different plant sources on survival and development of Phthorimaea opercullela (Zeller) larvae","authors":"I. Idris, M. Naddaf, H. Harmalani, R. Alshater, R. Alsafadi","doi":"10.2478/hppj-2023-0008","DOIUrl":"https://doi.org/10.2478/hppj-2023-0008","url":null,"abstract":"Summary The study examined the effect of nano-silica extracted from two different plant sources on the survival and development of the potato tuber moth, Phthorimaea opercullela. The silica powder was derived from two different agricultural byproducts, olive stones and corncobs. Characterization by X-ray diffraction revealed that the extracted powder has an amorphous silica phase. The nitrogen adsorption-desorption measurements revealed that both extracted and treated silica have mesoporous structure, with a specific surface area of around 300 m2/g and 270 m2/g for the silica derived from olive stones and corncobs, respectively. The silica nanoparticles (SiO2 NPs) prepared from the silica derived from olive stοnes showed higher larvae mortality, pupae weight, and larval and pupal developmental time, compared to the silica derived from corncombs. The results show that the nano-silica derived from agriculture byproducts can be as effective as the synthetic insecticide (deltamethrin) utilized in control of the potato tuber moth, with lower environmental impact in terms of preventing pesticide residue accumulation. In addition, the efficiency of SiO2 NPs applications depends on the source of the silica nanoparticles and the applied concentration to achieve the optimum results for the pest control.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41994463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
New data on the parasitization of Aleurothrixus floccosus (Maskell) (Hemiptera: Aleyrodidae) in Greece 文章标题希腊絮状粉虱(半翅目:粉虱科)寄生性的新资料
Q3 Agricultural and Biological Sciences Pub Date : 2023-07-01 DOI: 10.2478/hppj-2023-0010
G. Stathas, N. Kavallieratos, L.N. Cheliotis, P. Skouras, M.V. Giakoumaki, P. Milonas
Summary Signiphora flavella (Girault) (Hymenoptera: Signiphoridae) was recorded in 2022 as a new parasitoid species of the serious pest of citrus Aleurothrixus floccosus (Maskell) in Greece, in two different areas, i.e., the provinces of Laconia and Messinia. Previously, Signiphora flavella was recorded in Greece parasitizing Hemiberlesia rapax (Comstock) and H. lataniae (Signoret) in early 60s. It is mainly a parasitoid species of scale insects belonging to Diaspididae (Hemiptera: Coccomorpha), whiteflies, or a hyperparasitoid of aphelinids.
在希腊拉科尼亚省和迈锡尼亚省,于2022年分别记录到一种新的寄生蜂,为柑橘严重害虫Aleurothrixus flocosus (Maskell)。早在上世纪60年代初,希腊就有报道称,黄花蓟马寄生于rapax圆蚧(Comstock)和lataniae (Signoret)。它主要是一种寄生性的介壳昆虫,属于介壳昆虫科(半翅目:介壳昆虫科),或者是一种超寄生性的介壳昆虫。
{"title":"New data on the parasitization of Aleurothrixus floccosus (Maskell) (Hemiptera: Aleyrodidae) in Greece","authors":"G. Stathas, N. Kavallieratos, L.N. Cheliotis, P. Skouras, M.V. Giakoumaki, P. Milonas","doi":"10.2478/hppj-2023-0010","DOIUrl":"https://doi.org/10.2478/hppj-2023-0010","url":null,"abstract":"Summary Signiphora flavella (Girault) (Hymenoptera: Signiphoridae) was recorded in 2022 as a new parasitoid species of the serious pest of citrus Aleurothrixus floccosus (Maskell) in Greece, in two different areas, i.e., the provinces of Laconia and Messinia. Previously, Signiphora flavella was recorded in Greece parasitizing Hemiberlesia rapax (Comstock) and H. lataniae (Signoret) in early 60s. It is mainly a parasitoid species of scale insects belonging to Diaspididae (Hemiptera: Coccomorpha), whiteflies, or a hyperparasitoid of aphelinids.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42024518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of in-vitro antifungal activity against Fusarium incarnatum of binary and ternary combinations of lemongrass, garlic and mustard oil-encapsulated lipid nanoemulsions 柠檬草、大蒜和芥菜油包封脂质纳米乳二元和三元组合体外抗褐变镰刀菌活性评价
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.2478/hppj-2023-0001
Minh-Hiep Nguyen, Thi-Ngoc-Mai Tran
Summary Encapsulated lipid nanoemulsions (EO-LNs) from garlic oil, lemongrass oil and mustard oil were prepared by a combined method of homogenization and sonication with the aim to generate highly effective formulations against Fusarium incarnatum (laboratory bioassays). Their combined binary and ternary formulations (preparations by mixing an equal volume ratio of each EO-LNs) were also tested. The synergistic/additive/antagonistic antifungal effect of the EOs (under nanoform) in their combined formulations was determined using the SynergyFinder software with the Bliss independence model. Results revealed the synergistic effect of the combined binary and ternary formulations of garlic oil-encapsulated LNs (NaG), lemongrass oil-encapsulated LNs (NaL) and mustard oil-encapsulated LNs (NaM). Furthermore, the ternary combination, at the same concentration of each constituent EO, had higher antifungal activity than the binary combinations. Nonetheless, at 600 times dilution the NaLG (binary combination) inhibited 96% the mycelial growth of F. incarnatum, which was significantly higher than the efficacy of NaMLG (ternary combination) in the same dilution. This could be possibly attributed to the 1.5-time higher concentration of each constituent EO in the binary combination compared to that in the ternary formulation. In addition, NaLG, even at the high EO concentration of 0.4 g/L, did not show any phytotoxicity symptoms on lettuce plants.
以大蒜油、柠檬草油和芥菜油为原料,采用均质和超声相结合的方法制备了胶囊状脂质纳米乳(o - lns),目的是制备出高效的抗镰状镰刀菌制剂(实验室生物测定)。他们的组合二元和三元配方(通过混合每一个EO-LNs等体积比的制剂)也进行了测试。利用协同/加性/拮抗抗真菌效果(纳米形式下),利用SynergyFinder软件和Bliss独立模型来确定其联合配方。结果表明,大蒜油包封LNs (NaG)、香茅油包封LNs (NaL)和芥菜油包封LNs (NaM)的二、三元复合配方具有协同效应。此外,在相同浓度下,三元组合比二元组合具有更高的抗真菌活性。然而,在600倍稀释条件下,NaLG(二元组合)对F. incarnatum菌丝生长的抑制作用为96%,显著高于相同稀释条件下的NaMLG(三元组合)。这可能是由于二元组合中各组分EO的浓度比三元组合高1.5倍。此外,即使在0.4 g/L的高浓度EO下,NaLG对生菜也没有表现出任何植物毒性症状。
{"title":"Evaluation of in-vitro antifungal activity against Fusarium incarnatum of binary and ternary combinations of lemongrass, garlic and mustard oil-encapsulated lipid nanoemulsions","authors":"Minh-Hiep Nguyen, Thi-Ngoc-Mai Tran","doi":"10.2478/hppj-2023-0001","DOIUrl":"https://doi.org/10.2478/hppj-2023-0001","url":null,"abstract":"Summary Encapsulated lipid nanoemulsions (EO-LNs) from garlic oil, lemongrass oil and mustard oil were prepared by a combined method of homogenization and sonication with the aim to generate highly effective formulations against Fusarium incarnatum (laboratory bioassays). Their combined binary and ternary formulations (preparations by mixing an equal volume ratio of each EO-LNs) were also tested. The synergistic/additive/antagonistic antifungal effect of the EOs (under nanoform) in their combined formulations was determined using the SynergyFinder software with the Bliss independence model. Results revealed the synergistic effect of the combined binary and ternary formulations of garlic oil-encapsulated LNs (NaG), lemongrass oil-encapsulated LNs (NaL) and mustard oil-encapsulated LNs (NaM). Furthermore, the ternary combination, at the same concentration of each constituent EO, had higher antifungal activity than the binary combinations. Nonetheless, at 600 times dilution the NaLG (binary combination) inhibited 96% the mycelial growth of F. incarnatum, which was significantly higher than the efficacy of NaMLG (ternary combination) in the same dilution. This could be possibly attributed to the 1.5-time higher concentration of each constituent EO in the binary combination compared to that in the ternary formulation. In addition, NaLG, even at the high EO concentration of 0.4 g/L, did not show any phytotoxicity symptoms on lettuce plants.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45880090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Unraveling the role of endophytic fungi in barley salt-stress tolerance 内生真菌在大麦耐盐性中的作用
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.2478/hppj-2023-0002
R. Kouadria, M. Bouzouina, B. Lotmani, S. Soualem
Summary Salinity is an agricultural and eco-environmental problem worldwide that decreases crop production. Endophytic fungi have been shown to improve plant tolerance to stressful conditions. The purpose of the paper is to examine the efficiency of Embellisia phragmospora, Fusarium equiseti and Fusarium graminearum to improve tolerance of pot-grown barley in greenhouse under different levels of soil salinity (2.5, 8 and 14dS/m) by estimating growth, relative water content, mineral nutrition, photosynthetic pigments biosynthesis, proline and sugar levels. Results showed that E. phragmospora increased barley emergence rate to 66.7% compared to 60% recorded by non-colonized barley under 14dS/m soil salinity. The tested endophytes increased barley root length, shoot and root dry weights under salt stress. Endophytic fungi reduced Na+ accumulation and improved K+ uptake in barely under salinity. Fusarium equiseti and F. graminearum-inoculated barley increased proline content under salinity. Fusarium graminearum-colonized barley showed the highest sugar content under salt stress. Our findings demonstrate the feasibility of endophytic fungi bio-inoculation in improvement of barley tolerance to salt stress, which qualify them to be a potent tool to provide substantial benefits to crops for sustainable agriculture.
盐度是一个全球性的农业和生态环境问题,会降低作物产量。内生真菌已被证明可以提高植物对胁迫条件的耐受性。本文通过估算生长、相对含水量、矿质营养、光合色素生物合成、脯氨酸和糖水平,研究了在不同土壤盐度(2.5、8和14dS/m)条件下,黄柏、马齿镰刀菌和禾谷镰刀菌在提高温室盆栽大麦耐性方面的效率。结果表明,在14dS/m的土壤盐度下,E.phragmospora使大麦的出苗率从未定植大麦的60%提高到66.7%。所测试的内生菌在盐胁迫下增加了大麦的根长、地上部和根干重。内生真菌在盐度较低的条件下减少了Na+的积累,提高了K+的吸收。马齿镰刀菌和禾谷镰刀菌接种的大麦在盐度下增加了脯氨酸含量。在盐胁迫下,禾谷镰刀菌定植大麦的含糖量最高。我们的研究结果证明了内生真菌生物接种在提高大麦对盐胁迫的耐受性方面的可行性,这使它们成为为可持续农业作物提供实质性利益的有力工具。
{"title":"Unraveling the role of endophytic fungi in barley salt-stress tolerance","authors":"R. Kouadria, M. Bouzouina, B. Lotmani, S. Soualem","doi":"10.2478/hppj-2023-0002","DOIUrl":"https://doi.org/10.2478/hppj-2023-0002","url":null,"abstract":"Summary Salinity is an agricultural and eco-environmental problem worldwide that decreases crop production. Endophytic fungi have been shown to improve plant tolerance to stressful conditions. The purpose of the paper is to examine the efficiency of Embellisia phragmospora, Fusarium equiseti and Fusarium graminearum to improve tolerance of pot-grown barley in greenhouse under different levels of soil salinity (2.5, 8 and 14dS/m) by estimating growth, relative water content, mineral nutrition, photosynthetic pigments biosynthesis, proline and sugar levels. Results showed that E. phragmospora increased barley emergence rate to 66.7% compared to 60% recorded by non-colonized barley under 14dS/m soil salinity. The tested endophytes increased barley root length, shoot and root dry weights under salt stress. Endophytic fungi reduced Na+ accumulation and improved K+ uptake in barely under salinity. Fusarium equiseti and F. graminearum-inoculated barley increased proline content under salinity. Fusarium graminearum-colonized barley showed the highest sugar content under salt stress. Our findings demonstrate the feasibility of endophytic fungi bio-inoculation in improvement of barley tolerance to salt stress, which qualify them to be a potent tool to provide substantial benefits to crops for sustainable agriculture.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47818562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the antibacterial activity of essential oil of Laurus nobilis against Pseudomonas syringae pv. phaseolicola and potential biocidal action 月桂精油对丁香假单胞菌的抗菌活性评价。Pharolicola及其潜在的生物杀灭作用
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.2478/hppj-2023-0005
S. Mamoucha, A. Prombona, A. Galeou
Summary With a continuously growing human population on our planet, the chemical burden of our environment is also growing. In addition, the resistance of microorganisms, like bacteria, to widely used chemicals is evident. Therefore, the application of bactericidal products that reduce the risks for development of resistance as well as the environment and human safety is of great benefit. In this work, we have screened the essential oil extracted from plants of Laurus nobilis L. (laurel) grown at the base of the Greek mountain Olympus for its antimicrobial activity against two strains of the Phaseolus vulgaris pathogen, Pseudomonas syringae pv. phaseolicola as well as human pathogenic bacteria (biocidal use). Our results, obtained with established methods, like Well diffusion and Disc diffusion assay, reveal that laurel essential oil is a very effective bacteriostatic and bactericidal agent. Importantly, the activity of laurel essential oil as growth inhibitor of the plant pathogen Pseudomonas syringae pv. phaseolicola is reported for the first time. This opens the field for more extended investigations regarding its use in crop protection. Additionally, the laurel essential oil tested showed significant antibacterial properties against several human pathogenic bacteria, namely Micrococcus luteus, Escherichia coli, Staphylococcus aureus and Bacillus subtilis.
摘要随着地球上人口的不断增长,我们环境的化学负担也在增加。此外,微生物,如细菌,对广泛使用的化学物质的抵抗力是显而易见的。因此,应用杀菌产品可以降低耐药性发展的风险,并对环境和人类安全大有裨益。在这项工作中,我们筛选了从生长在希腊奥林巴斯山脚下的月桂属植物中提取的精油,以测定其对两株菜豆病原体丁香假单胞菌pv的抗菌活性。Pharolicola以及人类致病菌(生物杀灭用途)。我们的结果表明,月桂精油是一种非常有效的抑菌和杀菌剂。重要的是,月桂精油作为植物病原体丁香假单胞菌pv的生长抑制剂的活性。phaseolicola是首次报道。这为进一步研究其在作物保护中的应用开辟了道路。此外,测试的月桂精油对几种人类致病菌,即黄微球菌、大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌表现出显著的抗菌性能。
{"title":"Evaluation of the antibacterial activity of essential oil of Laurus nobilis against Pseudomonas syringae pv. phaseolicola and potential biocidal action","authors":"S. Mamoucha, A. Prombona, A. Galeou","doi":"10.2478/hppj-2023-0005","DOIUrl":"https://doi.org/10.2478/hppj-2023-0005","url":null,"abstract":"Summary With a continuously growing human population on our planet, the chemical burden of our environment is also growing. In addition, the resistance of microorganisms, like bacteria, to widely used chemicals is evident. Therefore, the application of bactericidal products that reduce the risks for development of resistance as well as the environment and human safety is of great benefit. In this work, we have screened the essential oil extracted from plants of Laurus nobilis L. (laurel) grown at the base of the Greek mountain Olympus for its antimicrobial activity against two strains of the Phaseolus vulgaris pathogen, Pseudomonas syringae pv. phaseolicola as well as human pathogenic bacteria (biocidal use). Our results, obtained with established methods, like Well diffusion and Disc diffusion assay, reveal that laurel essential oil is a very effective bacteriostatic and bactericidal agent. Importantly, the activity of laurel essential oil as growth inhibitor of the plant pathogen Pseudomonas syringae pv. phaseolicola is reported for the first time. This opens the field for more extended investigations regarding its use in crop protection. Additionally, the laurel essential oil tested showed significant antibacterial properties against several human pathogenic bacteria, namely Micrococcus luteus, Escherichia coli, Staphylococcus aureus and Bacillus subtilis.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47065551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
First record of the scale insect Stotzia ephedrae in Greece 希腊首次记录鳞虫麻黄
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.2478/hppj-2023-0003
G. Stathas, E. Kartsonas, A. Darras
Summary The scale insect Stotzia ephedrae (Newstead) (Hemiptera: Coccomorpha: Coccidae) was recorded for the first time in Greece οn 20 April 2021. It was found on Ephedra foeminea Forssk. (Ephedraceae: Gnetales) in Athens. From preliminary studies it was found that S. ephedrae is an oviparous, bi-parental species completing one generation per year.
摘要2021年4月20日,希腊首次记录到鳞片虫Stotzia ephedrae(Newstead)(半翅目:Coccomorpha:Coccidae)。它是在麻黄上发现的。(麻黄素科:Gnetales)在雅典。初步研究发现,麻黄是一种产卵的双亲物种,每年完成一代。
{"title":"First record of the scale insect Stotzia ephedrae in Greece","authors":"G. Stathas, E. Kartsonas, A. Darras","doi":"10.2478/hppj-2023-0003","DOIUrl":"https://doi.org/10.2478/hppj-2023-0003","url":null,"abstract":"Summary The scale insect Stotzia ephedrae (Newstead) (Hemiptera: Coccomorpha: Coccidae) was recorded for the first time in Greece οn 20 April 2021. It was found on Ephedra foeminea Forssk. (Ephedraceae: Gnetales) in Athens. From preliminary studies it was found that S. ephedrae is an oviparous, bi-parental species completing one generation per year.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46910237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First record of the scale insect Lineaspis striata on Picea glauca “Conica” 灰皮云杉“圆锥形”上鳞虫条纹纹纹皮虫的首次记录
Q3 Agricultural and Biological Sciences Pub Date : 2023-01-01 DOI: 10.2478/hppj-2023-0004
G. Stathas, A. Darras, E. Kartsonas
Summary The scale insect Lineaspis striata (Newstead) (Hemiptera: Coccomorpha: Diaspididae) was recorded for the first time infesting a species of the family Pinaceae. Heavily infested ornamental shrubs of Picea glauca “Conica” by pre-ovipostiting and oviposting female adults of L. striata, were found in March 2018 and by a minor population consisting of pre-ovipositing female adults in February 2021, in Attica, Greece. The scale was settled on the base of the needles of the host plant.
摘要首次记录到鳞虫Lineaspis striata(Newstead)(半翅目:蚧形目:双蛛科)侵扰松科一种。2018年3月,在希腊阿提卡发现了白皮云杉(Picea glauca“Conica”)的观赏灌木,其产卵前和产卵雌性成虫严重侵扰,2021年2月,由产卵前雌性成虫组成的小种群也发现了这种灌木。水垢沉淀在寄主植物的针叶上。
{"title":"First record of the scale insect Lineaspis striata on Picea glauca “Conica”","authors":"G. Stathas, A. Darras, E. Kartsonas","doi":"10.2478/hppj-2023-0004","DOIUrl":"https://doi.org/10.2478/hppj-2023-0004","url":null,"abstract":"Summary The scale insect Lineaspis striata (Newstead) (Hemiptera: Coccomorpha: Diaspididae) was recorded for the first time infesting a species of the family Pinaceae. Heavily infested ornamental shrubs of Picea glauca “Conica” by pre-ovipostiting and oviposting female adults of L. striata, were found in March 2018 and by a minor population consisting of pre-ovipositing female adults in February 2021, in Attica, Greece. The scale was settled on the base of the needles of the host plant.","PeriodicalId":39459,"journal":{"name":"Hellenic Plant Protection Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44892695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Hellenic Plant Protection Journal
全部 Acc. Chem. Res. ACS Applied Bio Materials ACS Appl. Electron. Mater. ACS Appl. Energy Mater. ACS Appl. Mater. Interfaces ACS Appl. Nano Mater. ACS Appl. Polym. Mater. ACS BIOMATER-SCI ENG ACS Catal. ACS Cent. Sci. ACS Chem. Biol. ACS Chemical Health & Safety ACS Chem. Neurosci. ACS Comb. Sci. ACS Earth Space Chem. ACS Energy Lett. ACS Infect. Dis. ACS Macro Lett. ACS Mater. Lett. ACS Med. Chem. Lett. ACS Nano ACS Omega ACS Photonics ACS Sens. ACS Sustainable Chem. Eng. ACS Synth. Biol. Anal. Chem. BIOCHEMISTRY-US Bioconjugate Chem. BIOMACROMOLECULES Chem. Res. Toxicol. Chem. Rev. Chem. Mater. CRYST GROWTH DES ENERG FUEL Environ. Sci. Technol. Environ. Sci. Technol. Lett. Eur. J. Inorg. Chem. IND ENG CHEM RES Inorg. Chem. J. Agric. Food. Chem. J. Chem. Eng. Data J. Chem. Educ. J. Chem. Inf. Model. J. Chem. Theory Comput. J. Med. Chem. J. Nat. Prod. J PROTEOME RES J. Am. Chem. Soc. LANGMUIR MACROMOLECULES Mol. Pharmaceutics Nano Lett. Org. Lett. ORG PROCESS RES DEV ORGANOMETALLICS J. Org. Chem. J. Phys. Chem. J. Phys. Chem. A J. Phys. Chem. B J. Phys. Chem. C J. Phys. Chem. Lett. Analyst Anal. Methods Biomater. Sci. Catal. Sci. Technol. Chem. Commun. Chem. Soc. Rev. CHEM EDUC RES PRACT CRYSTENGCOMM Dalton Trans. Energy Environ. Sci. ENVIRON SCI-NANO ENVIRON SCI-PROC IMP ENVIRON SCI-WAT RES Faraday Discuss. Food Funct. Green Chem. Inorg. Chem. Front. Integr. Biol. J. Anal. At. Spectrom. J. Mater. Chem. A J. Mater. Chem. B J. Mater. Chem. C Lab Chip Mater. Chem. Front. Mater. Horiz. MEDCHEMCOMM Metallomics Mol. Biosyst. Mol. Syst. Des. Eng. Nanoscale Nanoscale Horiz. Nat. Prod. Rep. New J. Chem. Org. Biomol. Chem. Org. Chem. Front. PHOTOCH PHOTOBIO SCI PCCP Polym. Chem.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1