Nano Today最新文献_第3页

Nanoengineered living macrophages as ultrasound imaging-trackable cell extinguishers inhibit pyroptosis in atherosclerotic plaque 纳米工程活巨噬细胞作为超声成像可追踪细胞灭火剂抑制动脉粥样硬化斑块的焦亡

IF 10.9 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Today

Pub Date : 2026-01-05 DOI: 10.1016/j.nantod.2025.102972

Zheng Zhang , Jiahuan Xu , Meiqi Chang , Xinran Song , Wei Feng , Li Ding , Yu Chen , Bo Zhang

Pyroptosis is a form of programmed cell death (PCD) that bolsters local and systemic inflammatory responses, which is closely associated with atherosclerosis (AS) progression and plaque vulnerability. Although current treatments have achieved satisfactory progress in suppressing pyroptosis, there are significant challenges remaining in effectively alleviating plaque burden due to issues including poor site specificity, extensive systemic distribution, and undesirable side effects. Herein, intelligent living cell-based drug delivery systems (LC-DDSs) targeting AS intervention with distinct functions was designed by loading disulfiramand (DSF) and the phase-transition perfluorohexane (PFH) onto the bovine serum albumin (BSA)-modified Co₂Mo₃O₈ nanosheets, followed by phagocytosis into macrophages to construct PDCos@Ms. Upon ultrasonic irradiation, these nanoengineered PDCos@Ms can generate microbubbles, enabling controlled release of their therapeutic payloads. This mechanism effectively ameliorates AS progression and enhances plaque stability by suppressing programmed cell pyroptosis and promoting an anti-inflammatory macrophage phenotype through scavenging reactive oxygen species, reducing the expression of pyroptosis-associated proteins and diminishing the leakage of inflammatory factors. Meanwhile, the PFH integrated within the system facilitate real-time ultrasonic imaging, allowing for image-guided atherosclerotic therapy. The proposed biomimetic therapeutic strategy holds significant potential for efficient plaque diminishing and provides a distinct paradigm of LC-DDSs for AS management.

焦亡是程序性细胞死亡（PCD）的一种形式，可增强局部和全身炎症反应，与动脉粥样硬化（AS）进展和斑块易感性密切相关。尽管目前的治疗方法在抑制焦下垂方面取得了令人满意的进展，但由于部位特异性差、广泛的全身分布和不良副作用等问题，在有效减轻斑块负担方面仍存在重大挑战。本文通过在牛血清白蛋白（BSA）修饰的Co2Mo3O8纳米片上加载二硫酰胺（DSF）和相变全氟己烷（PFH），然后吞噬巨噬细胞构建PDCos@Ms，设计了具有不同功能的靶向AS干预的智能活细胞药物递送系统（lc - dss）。在超声波照射下，这些纳米工程PDCos@Ms可以产生微泡，从而控制其治疗有效载荷的释放。该机制通过清除活性氧抑制程序性细胞热亡，促进抗炎巨噬细胞表型，减少热亡相关蛋白的表达，减少炎症因子的泄漏，有效改善AS的进展，增强斑块的稳定性。同时，系统内集成的PFH有助于实时超声成像，从而实现图像引导的动脉粥样硬化治疗。提出的仿生治疗策略具有有效减少斑块的巨大潜力，并为AS管理提供了lc - dds的独特范例。

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引用次数: 0

Inflammatory environment-responsive nanomicelles targeting CD44 alleviate allergic asthma by inhibiting the TLR4/MyD88-NFκB pathway 靶向CD44的炎症环境响应性纳米胶束通过抑制TLR4/MyD88-NFκB通路减轻过敏性哮喘

IF 10.9 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Today

Pub Date : 2026-01-02 DOI: 10.1016/j.nantod.2025.102973

Tianwei Lan , Xiaoping Guo , Chang Liu , Pai Wang , Shichen Zhang , Lei Guo , Siyuan Wang , Lu Wang , Rui Zhou , Xinran Yan , Baiqiao Chen , Mujie Kan , Fang Wang , Caina Xu

Allergic asthma (AAS) is a chronic inflammatory airway disease. While zileuton (ZIL) has the efficient ability to relieve AAS symptoms, its systemic, untargeted administration could lead to adverse neurological effects. Thus, we constructed a drug combination nanomicelle (NMs) platform, H(BZP)-NMs, to reach synergistic effect therapy. It was comprised of a hydrophilic end formed from methoxy poly(ethylene glycol) (mPEG) and poly(L-glutamic acid) (PGlu), and a ROS/pH dual-sensitive hydrophobic core. The core was created by coupling 4-(hydroxymethyl)phenylboronic acid (HPBA) to betamethasone (BMZ). Zileuton (ZIL) was strategically loaded in the core. Hyaluronic acid (HA) functionalization further enabled CD44-targeting ability. In pathological microenvironments, the boronate ester bonds undergo responsive cleavage, causing the parallel delivery of the drugs. The slow-release profile addressed the need for rapid early-stage control and prolonged late-stage relief. In vivo, H(BZP)-NMs significantly decreased Penh values, lowered the IL-4, IL-5, IL-13, and LTB4 levels, and ameliorated airway pathological injuries. Given the molecular docking and verification of network pharmacology, these effects were associated with the dual-drug synergistic inhibition of the TLR4-MyD88-NF-κB p65 signals. Collectively, this study shows that H(BZP)-NMs are an inventive nanomedicine approach that unifies targeted delivery and extended co-release, offering promise for accurate and synergistic treatment of AAS.

过敏性哮喘（AAS）是一种慢性炎症性气道疾病。虽然zileuton （ZIL）具有有效缓解AAS症状的能力，但其系统性、非靶向给药可能导致不良的神经系统反应。因此，我们构建了药物联合纳米微球（NMs）平台H(BZP)-NMs，以达到协同效应治疗。它由甲氧基聚乙二醇（mPEG）和聚l-谷氨酸（PGlu）形成的亲水端和ROS/pH双敏感疏水核组成。4-（羟甲基）苯基硼酸（HPBA）与倍他米松（BMZ）偶联形成核心。Zileuton （ZIL）战略性地装载在核心位置。透明质酸（HA）功能化进一步激活了cd44靶向能力。在病理微环境中，硼酸酯键发生反应性裂解，导致药物平行递送。缓释型解决了快速早期控制和延长晚期缓解的需要。在体内，H(BZP)-NMs显著降低Penh值，降低IL-4、IL-5、IL-13和LTB4水平，改善气道病理损伤。基于网络药理学的分子对接和验证，这些作用可能与双药协同抑制TLR4-MyD88-NF-κB p65信号有关。总的来说，本研究表明H(BZP)-NMs是一种创造性的纳米医学方法，它将靶向给药和延长共释放结合起来，为精确和协同治疗AAS提供了希望。

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引用次数: 0

Biomineralization-inspired nanosheet transformation potentiates cancer therapy through precise embolization and antitumor immunity restoration 生物矿化激发的纳米片转化通过精确栓塞和抗肿瘤免疫恢复来增强癌症治疗

IF 10.9 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Today

Pub Date : 2025-12-31 DOI: 10.1016/j.nantod.2025.102971

Kangli Guo , Ling Liang , Ying Liu , Xiaoyi Zhao , Bo Zhi Chen , Dandan Sui , Xin Dong Guo , Fu-Jian Xu , Nana Zhao

Tumor vasculature targeting therapy represents a promising approach to inhibit tumor growth by disrupting the supply of nutrients and oxygen. However, achieving tumor-specific embolization remains a great challenge while the severe disruption of the vascular supply is supposed to aggravate tumor immunosuppression. Inspired by natural biomineralization process of magnesium phosphate, we developed a novel approach combining Mg(OH)₂ nanosheets with microneedles (MNs) for precise tumor embolization and enhanced immunotherapy. Triggered by elevated concentrations of hydrogen ions and phosphate in the tumor microenvironment (TME), Mg(OH)₂ nanosheets undergo a transformation into biomineralized magnesium phosphate precipitates for tumor-specific embolization. Furthermore, these nanosheets exhibit intrinsic immunomodulatory properties, capable of reversing immunosuppression by neutralizing the acidic TME. Additionally, they induce dendritic cell maturation and oxidative stress in tumor cells, triggering immunogenic cell death and eliciting robust antitumor immune responses. To further enhance the efficiency, the MN platform enables co-delivery of the antiangiogenic drug sunitinib with Mg(OH)₂ nanosheets. The satisfactory antitumor effects of the Mg(OH)₂-MNs platform in vivo were validated in a 4T1 tumor-bearing mice model. Transcriptomics analysis confirmed the activation of strong antitumor immunity and successful immunosuppression reversal. This study proposes a promising biomineralization-inspired strategy to construct immunomodulatory nanomaterials for tumor-specific embolization and enhanced immunotherapy.

肿瘤血管靶向治疗是一种很有前途的方法，通过破坏营养和氧气的供应来抑制肿瘤的生长。然而，实现肿瘤特异性栓塞仍然是一个巨大的挑战，而血管供应的严重中断可能会加重肿瘤的免疫抑制。受天然磷酸镁生物矿化过程的启发，我们开发了一种将Mg(OH)2纳米片与微针（MNs）结合的新方法，用于精确肿瘤栓塞和增强免疫治疗。在肿瘤微环境（TME）中氢离子和磷酸盐浓度升高的触发下，Mg(OH)2纳米片转化为生物矿化磷酸镁沉淀，用于肿瘤特异性栓塞。此外，这些纳米片表现出固有的免疫调节特性，能够通过中和酸性TME来逆转免疫抑制。此外，它们诱导肿瘤细胞中的树突状细胞成熟和氧化应激，引发免疫原性细胞死亡并引发强大的抗肿瘤免疫反应。为了进一步提高效率，MN平台可以将抗血管生成药物舒尼替尼与Mg(OH)2纳米片共同递送。在4T1荷瘤小鼠模型中验证了Mg(OH)2-MNs平台的体内抗肿瘤效果。转录组学分析证实了强大的抗肿瘤免疫激活和成功的免疫抑制逆转。本研究提出了一种有前途的生物矿化启发策略，构建用于肿瘤特异性栓塞和增强免疫治疗的免疫调节纳米材料。

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引用次数: 0

Metal-polyphenol networks-mediated Au-Pt nanoplatform for dual-mode LDI-MS and colorimetric diagnosis of urinary stones 金属-多酚网络介导的Au-Pt纳米平台用于双模LDI-MS和尿路结石的比色诊断

IF 10.9 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Today

Pub Date : 2025-12-29 DOI: 10.1016/j.nantod.2025.102965

Qi Sang , Yinpeng Liu , Yanyan Li , Wanshan Liu , Tong Hu , Ruimin Wang , Zhebin Du , Yuning Wang , Kun Qian

Urinary stones (US) are common urological disease with a high incidence and a risk of comorbidities. An accurate and high-throughput strategy is greatly needed for US diagnosis. Herein, we designed metal-polyphenol networks mediated Au-Pt hybrid nanoparticles (AuMPN-Pt HNPs) and fabricated a dual-functional nanoplatform integrating LDI-MS analysis and colorimetric assay for US diagnosis and subtype classification. The AuMPN-Pt HNPs exhibited superior LDI-MS performance with high sensitivity (limit of detection (LOD) < 2 µM) and good MS signal reproducibility (coefficients of variation (CVs) < 10 %) for accurate detection of metabolites. Meanwhile, AuMPN-Pt HNPs presented excellent peroxidase (POD)-like activity, allowing for direct and rapid detection of uric acid (UA) assisted by uricase with a LOD of 9.43 µM. Using the AuMPN-Pt HNPs assisted LDI-MS platform, we successfully achieved non-invasive US diagnosis by metabolic profiling of urine samples from US patients and the healthy controls (HCs), as well as subtype discrimination of calcium oxalate stones (CaOx stones) from UA stones by combining analysis of metabolic fingerprints from serum and urine samples. The subtype discrimination results were further verified by colorimetric platform for serum UA level analysis. The dual-functional nanoplatform exhibited outstanding sensitivity and specificity, providing innovative insights into disease diagnosis and subtype differentiation.

尿路结石（US）是一种常见的泌尿系统疾病，具有高发病率和合并症风险。美国迫切需要一种准确、高通量的诊断策略。在此，我们设计了金属-多酚网络介导的Au-Pt杂化纳米粒子（AuMPN-Pt HNPs），并构建了一个双功能纳米平台，集成了LDI-MS分析和比色分析，用于美国诊断和亚型分类。AuMPN-Pt HNPs具有较高的灵敏度（检出限（LOD） <； 2 µM）和良好的质谱信号重现性（变异系数（cv） <； 10 %），可准确检测代谢物。同时，AuMPN-Pt HNPs表现出优异的过氧化物酶（POD）样活性，允许在尿酸酶的辅助下直接快速检测尿酸（UA）， LOD为9.43 µM。利用AuMPN-Pt HNPs辅助LDI-MS平台，我们通过对美国患者和健康对照（hc）的尿液样本进行代谢谱分析，成功地实现了无创US诊断，并通过结合血清和尿液样本的代谢指纹分析，成功地区分了草酸钙结石（CaOx结石）和UA结石的亚型。血清UA水平分析比色平台进一步验证亚型区分结果。双功能纳米平台表现出出色的敏感性和特异性，为疾病诊断和亚型分化提供了创新的见解。

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引用次数: 0

Development of early diagnostic strategies by targeting CXCR3 following heart transplantation 心脏移植后CXCR3靶向早期诊断策略的发展

IF 10.9 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Today

Pub Date : 2025-12-29 DOI: 10.1016/j.nantod.2025.102966

Yuan Chang , Peiyuan Li , Zhe Sun , Weiteng Wang , Xiao Chen , Xiumeng Hua , Yixuan Sheng , Ningning Zhang , Qian Zhao , Kai Xing , Xingchao Du , Mengda Xu , Jiangping Song

Early diagnosis and treatment of acute rejection are crucial for prolonging graft survival in heart transplantation, yet no highly effective method exists for its timely detection. In this study, we identified CXCR3 as a T cell-specific biomarker via single-cell RNA sequencing of cardiac allografts and developed a nanoparticle-based fluorescent probe (CXCR3-NP) targeting CXCR3 to evaluate its diagnostic efficacy using in vivo imaging. Results demonstrated upregulated CXCR3 expression in T cells within acutely rejected tissues, validated in murine models and clinical samples, and the probe effectively bound to CXCR3 + T cells with fluorescence intensity increasing progressively during rejection. Furthermore, CXCR3-blocking antibody treatment alleviated acute rejection and reduced CXCR3 + T cell infiltration, leading to a significant decrease in CXCR3-NP fluorescence in grafts, thereby confirming the probe's high sensitivity in detecting diminished immune cell activity. This study developed a CXCR3-specific fluorescent probe for diagnosing acute rejection, with its sensitivity validated in a murine heart transplantation model showing alleviated acute rejection. Our findings provide novel insights into diagnostic strategies for cardiac allograft rejection.

心脏移植急性排斥反应的早期诊断和治疗对于延长移植物存活至关重要，但目前尚无有效的方法及时发现。在这项研究中，我们通过心脏同种异体移植的单细胞RNA测序确定了CXCR3是一种T细胞特异性生物标志物，并开发了一种靶向CXCR3的纳米颗粒荧光探针（CXCR3- np），通过体内成像评估其诊断效果。结果显示，急性排斥组织内的T细胞中CXCR3表达上调，在小鼠模型和临床样本中得到验证，并且探针有效结合CXCR3 + T细胞，荧光强度在排斥过程中逐渐增加。此外，CXCR3阻断抗体治疗减轻了急性排斥反应，减少了CXCR3 + T细胞浸润，导致移植物中CXCR3- np荧光显著降低，从而证实了探针在检测免疫细胞活性降低方面的高灵敏度。本研究开发了一种诊断急性排斥反应的cxcr3特异性荧光探针，其敏感性在小鼠心脏移植模型中得到验证，显示急性排斥反应减轻。我们的发现为心脏异体移植排斥反应的诊断策略提供了新的见解。

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引用次数: 0

Biohybrid decellularized peritumoral tissue hydrogel for liver failure treatment 生物杂化肿瘤周围组织水凝胶用于肝衰竭治疗

IF 10.9 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Today

Pub Date : 2025-12-24 DOI: 10.1016/j.nantod.2025.102968

Dongxue Ge , Zhongxia Wang , Dayu Chen , Feng Li , Haozhen Ren

Tissue engineering is considered a promising therapeutic strategy for acute liver failure (ALF), yet it is currently limited by the lack of natural scaffold materials and effective regeneration promoting approaches. Here, we propose a therapeutic strategy using a biohybrid decellularized peritumoral tissue hydrogel loaded with stem cell exosomes (exos) are applied to treat liver failure. Specifically, tissue samples obtained during clinical procedures are typically discarded as medical waste but can serve as natural scaffolds for therapeutic applications. Benefiting from a mild decellularization process, the decellularized peritumoral tissue retains amounts of bioactive substances while maintaining low immunogenicity. Moreover, induced pluripotent stem cell-derived hepatocytes (iPS-Heps) cultured within the decellularized extracellular matrix (dECM) hydrogel exhibit robust metabolic activity. More importantly, the incorporation of mesenchymal stem cells (MSCs) exos enables the effective integration of their inherent regenerative cytokines into the hydrogel. In vitro studies demonstrate that the hydrogel expresses high levels of liver functions, such as albumin (ALB) and the Cytochrome P450 proteins (CYP) family. In a D-galactosamine-induced liver failure rat model, the exos-loaded dECM hydrogel considerably improves survival rates, alleviates liver necrosis, and suppresses local inflammatory responses. This approach not only preserves bioactive substances from the tissue but also enhances metabolic activity in hepatocytes, thereby offering potential for improved clinical outcomes.

组织工程被认为是一种很有前途的治疗急性肝衰竭（ALF）的策略，但目前由于缺乏天然支架材料和有效的再生促进方法而受到限制。在这里，我们提出了一种治疗策略，使用装载干细胞外泌体（exos）的生物杂交脱细胞肿瘤周围组织水凝胶来治疗肝衰竭。具体来说，在临床过程中获得的组织样本通常作为医疗废物丢弃，但可以作为治疗应用的天然支架。得益于温和的脱细胞过程，脱细胞的肿瘤周围组织在保持低免疫原性的同时保留了大量的生物活性物质。此外，在脱细胞细胞外基质（dECM）水凝胶中培养的诱导多能干细胞源性肝细胞（iPS-Heps）表现出强大的代谢活性。更重要的是，间充质干细胞（MSCs）外显子的掺入使其固有的再生细胞因子有效地整合到水凝胶中。体外研究表明，水凝胶表达高水平的肝功能，如白蛋白（ALB）和细胞色素P450蛋白（CYP）家族。在d -半乳糖胺诱导的肝衰竭大鼠模型中，外源性负载的dECM水凝胶显著提高了存活率，减轻了肝坏死，并抑制了局部炎症反应。这种方法不仅保留了组织中的生物活性物质，而且增强了肝细胞的代谢活性，从而为改善临床结果提供了潜力。

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引用次数: 0

A self-delivering antimicrobial peptide hydrogel for treatment of staphylococcal infections in orthopedic implants 一种用于治疗骨科植入物中葡萄球菌感染的自传递抗菌肽水凝胶

IF 10.9 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Today

Pub Date : 2025-12-24 DOI: 10.1016/j.nantod.2025.102963

Tingting Li , Qipeng Yan , Yu Chen , Binwen Yuan , HongKun Hu , Dan Yuan , Weijun Tang , Wenbin Liu , Junfeng Shi

Implant-associated infections (IAIs) remain a major cause of implant failure, necessitating the development of effective anti-infection coatings for orthopedic implants. Here, we discovered a penetratin derived antimicrobial peptide (AMP) that spontaneously self-assembles into a mechanically rigid hydrogel with elongated fibrils by forming a triple helix structure. Molecular simulations revealed that intermolecular cation-π and π-π interactions, along with interfibrillar hydrogen bonds and electrostatic salt bridges, are essential for hydrogelation. The resulting hydrogel exhibited high biocompatibility, sustained AMP release, and potent broad-spectrum antimicrobial activity. Titanium sheets coated with resultant hydrogel effectively inhibited Staphylococcus aureus (S. aureus) growth for 7 days in vitro. Furthermore, the hydrogel demonstrated superior anti-infection efficacy as an implant coating, promoting osseointegration and mineralization in a rat model of osteomyelitis induced by implant infection. This work highlights the potential of self-delivery antibacterial peptide hydrogel as a promising platform for prevention of implant infections and advancing orthopedic implant technologies.

种植体相关感染（IAIs）仍然是导致种植体失败的主要原因，因此需要开发有效的骨科种植体抗感染涂层。在这里，我们发现了一种穿透素衍生的抗菌肽（AMP），它通过形成三螺旋结构，自发地自组装成具有细长原纤维的机械刚性水凝胶。分子模拟表明，分子间阳离子-π和π-π相互作用，以及纤维间氢键和静电盐桥对氢凝胶化至关重要。所得水凝胶具有高的生物相容性、持久的AMP释放和有效的广谱抗菌活性。制备的水凝胶包覆钛片可有效抑制金黄色葡萄球菌（S. aureus）体外生长7天。此外，水凝胶作为种植体涂层表现出优异的抗感染功效，促进种植体感染引起的骨髓炎大鼠模型的骨整合和矿化。这项工作强调了自递送抗菌肽水凝胶作为预防种植体感染和推进骨科种植体技术的有前途的平台的潜力。

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引用次数: 0

Dynamic hydration layer and ion-release synergy in coatings: Unraveling the key to sustainable anti-scaling during boiling and energy conservation 涂料中的动态水合层和离子释放协同作用：揭示沸煮过程中可持续防结垢和节能的关键

IF 10.9 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Today

Pub Date : 2025-12-22 DOI: 10.1016/j.nantod.2025.102964

Ran Zhao , Jianmin Gu , Zhihao Shang , Xiaopeng Cheng , Chunxiao Liang , Desong Wang , Shutao Wang , Jingxin Meng

Scale deposition severely degrades the heat-transfer efficiency of industrial equipment, leading to substantial energy wastage. While several advanced scalephobic surfaces have been developed to mitigate scaling, their poor stability under harsh operating conditions hinders practical implementation. Herein, we report a dynamic hydration layer-synergistic ion-releasing (DHLIR) coating that exhibits exceptional energy-saving potential, enabled by ultrahigh scale resistance under boiling conditions. The DHLIR coating was facilely fabricated via a combined spraying and heat-treatment process, utilizing aluminum phosphate (AP) as the inorganic binder, titanium dioxide (TiO₂) nanoparticles as the structural reinforcement phase, and hexagonal boron nitride (hBN) nanosheets as the thermally conductive filler. Notably, the superior scalephobic performance of the DHLIR coating stems from two key mechanisms: the surface hydration layer minimizes mineral ion adsorption, while the released scale inhibitors reduce scale adhesion. Furthermore, the DHLIR coating demonstrates robust durability under extreme environments, including high temperatures, abrasion, and corrosive liquids. In a simulated industrial boiler setup, the DHLIR coating conserved approximately 15.3 % of energy compared to stainless steel. This work thus presents a promising strategy for anti-scaling and energy conservation in boiling environments.

结垢严重降低了工业设备的传热效率，造成了大量的能源浪费。虽然已经开发了几种先进的防垢表面来减轻结垢，但它们在恶劣操作条件下的稳定性差阻碍了实际实施。在此，我们报道了一种动态水化层协同离子释放（DHLIR）涂层，该涂层在沸腾条件下具有超高的抗垢性，具有卓越的节能潜力。以磷酸铝（AP）为无机粘结剂，二氧化钛（tio2）纳米颗粒为结构增强相，六方氮化硼（hBN）纳米片为导热填料，采用喷涂与热处理相结合的方法制备了DHLIR涂层。值得注意的是，DHLIR涂层优越的阻垢性能源于两个关键机制：表面水化层最大限度地减少了矿物离子的吸附，而释放的阻垢剂减少了垢的粘附。此外，DHLIR涂层在极端环境下（包括高温、磨损和腐蚀性液体）表现出强大的耐久性。在模拟工业锅炉设置中，与不锈钢相比，DHLIR涂层节省了大约15.3% %的能量。因此，这项工作提出了一种在沸腾环境中抗结垢和节能的有前途的策略。

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引用次数: 0

A glucose-activated cascade oxygen release hydrogel wound dressing for microenvironment regulation of MRSA-infected diabetic wounds 葡萄糖激活级联释氧水凝胶创面敷料对mrsa感染糖尿病创面微环境的调节作用

IF 10.9 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Today

Pub Date : 2025-12-18 DOI: 10.1016/j.nantod.2025.102962

Huiru Xu , Xin Zhao , Jiaxin Wang , Yutong Yang , Shengfei Huang , Meng Li , Baolin Guo

Diabetic wounds are faced with a severe microenvironment of hyperglycemia, excessive ROS, and hypoxia. Developing targeted diabetic wound dressings that can improve this microenvironment to achieve efficient wound healing is highly anticipated. Here, a glucose-activated oxygen-release hydrogel loaded with cascaded nanozymes is designed to regulate wound microenvironment and promote MRSA-infected diabetic wound healing. Specifically, GOx@Zn-MOF-74 and MnO₂ are loaded into a hydrogel (HP-LT/G/M) formed by phenyl borate-grafted hyaluronic acid (HP) and 3,4,5-trihydroxybenzoic acid-grafted lysozyme (LT) via phenyl borate-ester bonds. In response to the hyperglycemia and excessive ROS wound microenvironment, the hydrogel releases these two nanozymes. GOx@Zn-MOF-74 can oxidize glucose into gluconic acid and H₂O₂ under the action of O₂, while MnO₂ converts endogenous and generated H₂O₂ into O₂. The cascade reaction can provide oxygen for subsequent glucose oxidation, accelerate the reaction process, improve wound hypoxia, consume glucose, and scavenge excessive ROS. In MRSA-infected full-skin defect model of diabetic mice, HP-LT/G/M hydrogel significantly accelerated wound closure, reduced inflammation, alleviated hypoxia, and promoted collagen deposition and angiogenesis. In summary, the glucose-activated oxygen release hydrogel with nanozyme cascade reaction responds to the wound microenvironment to achieve glucose decomposition, ROS scavenge, and oxygen release, showing great potential in diabetic wound management.

糖尿病创面面临着高血糖、活性氧过多、缺氧等严重的微环境。开发有针对性的糖尿病伤口敷料，可以改善这种微环境，以实现有效的伤口愈合是备受期待的。本研究设计了一种葡萄糖活化的氧释放水凝胶，负载级联纳米酶来调节伤口微环境，促进mrsa感染的糖尿病伤口愈合。具体来说，GOx@Zn-MOF-74和MnO2通过硼酸苯酯键加载到由接枝的透明质酸（HP）和接枝的3,4,5-三羟基苯甲酸溶菌酶（LT）形成的水凝胶（HP-LT/G/M）中。在高血糖和ROS过多的微环境下，水凝胶释放这两种纳米酶。GOx@Zn-MOF-74可以在O2的作用下将葡萄糖氧化为葡萄糖酸和H2O2，而MnO2将内源性和生成的H2O2转化为O2。级联反应可以为后续的葡萄糖氧化提供氧气，加速反应过程，改善创面缺氧，消耗葡萄糖，清除过量的ROS。在mrsa感染的糖尿病小鼠全皮肤缺损模型中，HP-LT/G/M水凝胶显著加速创面闭合，减轻炎症，缓解缺氧，促进胶原沉积和血管生成。综上所述，葡萄糖-活性氧释放水凝胶通过纳米酶级联反应响应伤口微环境，实现葡萄糖分解、活性氧清除和氧气释放，在糖尿病伤口管理中具有很大的潜力。

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引用次数: 0

A dual-targeting nanozyme platform integrating macrophage hitchhiking and TfR transcytosis for precision therapy of ischemic stroke 结合巨噬细胞搭便车和TfR胞吞的双靶向纳米酶平台用于缺血性卒中的精准治疗

IF 10.9 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Today

Pub Date : 2025-12-16 DOI: 10.1016/j.nantod.2025.102961

Weili Han , Feng Zeng , Kaikai Fu , Wenjie Lin , Zheng Cheng , Chang Li , Jun Chen , Yuanman Yu , Qiyong Mei

Ischemic stroke, a critical neurological disorder resulting from abrupt cerebral blood flow interruption, remains a leading cause of global mortality and chronic disability. Despite advancements in reperfusion therapies, many survivors still suffer significant neurological deficits, primarily attributed to ischemia-reperfusion injury-induced oxidative stress and neuroinflammation. To address these challenges, we designed cerium-curcumin hybrid nanoparticles (Ce-Cur NPs) by leveraging the coordination interaction between redox-active cerium ions and curcumin’s β-diketone moiety. These Ce-Cur NPs were engineered to alleviate ROS-mediated secondary injuries and promote neural repair by leveraging their remarkable ROS scavenging capabilities, which mimic enzymatic activities to effectively neutralize superoxide and hydroxyl radicals. Furthermore, M1 polarized macrophage-derived membranes were employed to enhance the targeted delivery of Ce-Cur NPs to ischemic regions, while transferrin receptor (TfR)-activated peptides enabled efficient receptor-mediated transcytosis across the blood-brain barrier. In vivo studies using a middle cerebral artery occlusion (MCAO) model demonstrated that TfR-M₁-Ce-Cur NPs successfully localized to the ischemic brain, significantly reduced infarct volume, and preserved neuronal integrity. Enhanced neurogenesis and improved functional recovery were observed, underscoring the therapeutic potential of Ce-Cur NPs as a versatile platform for ischemic stroke treatment. This approach provides a robust and evidence-based solution to enhance clinical outcomes for patients with ischemic stroke.

缺血性中风是一种由脑血流突然中断引起的严重神经系统疾病，仍然是全球死亡和慢性残疾的主要原因。尽管再灌注治疗取得了进展，但许多幸存者仍然遭受严重的神经功能障碍，主要归因于缺血-再灌注损伤引起的氧化应激和神经炎症。为了解决这些问题，我们设计了铈-姜黄素混合纳米粒子（Ce-Cur NPs），利用氧化还原活性的铈离子与姜黄素的β-二酮段之间的配位相互作用。这些Ce-Cur NPs被设计用于减轻ROS介导的继发性损伤，并通过利用其卓越的ROS清除能力促进神经修复，其模拟酶活性，有效中和超氧化物和羟基自由基。此外，M1极化巨噬细胞来源的膜被用于增强Ce-Cur NPs对缺血区域的靶向递送，而转铁蛋白受体（TfR）激活的肽能够有效地通过血脑屏障实现受体介导的胞质转运。使用大脑中动脉闭塞（MCAO）模型的体内研究表明，TfR-M1-Ce-Cur NPs成功定位于缺血脑，显着减少梗死体积，并保持神经元完整性。观察到增强的神经发生和改善的功能恢复，强调Ce-Cur NPs作为缺血性卒中治疗的多功能平台的治疗潜力。这种方法提供了一个强大的和基于证据的解决方案，以提高缺血性卒中患者的临床结果。

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