Pub Date : 2025-03-30DOI: 10.1016/j.nantod.2025.102745
Ali Akbar Ashkarran
Protein/biomolecular corona (PC) is a layer of biomolecules (mainly proteins) that forms around the nanoparticles (NPs) after exposure to biological environments (e.g., blood). The dynamic nature of the PC formation allows enrichment of specific proteins particularly low abundance proteins and enables capturing disease-specific proteins on the surface of the NPs for biomarker discovery. However, identification of crucial proteins with high diagnostic values heavily depends on liquid chromatography mass spectrometry (LC-MS/MS) approach due to its sensitivity and specificity. Despite the widespread use of MS, there exist potential pitfalls in NPs’ PC analysis that may lead to misconduct in biomarker discovery studies. This opinion considers these pitfalls, providing insights into the challenges and strategies to mitigate misconduct in biomarker discovery using mass spectrometry techniques.
{"title":"Decentralized nanoparticle protein corona analysis may misconduct biomarker discovery","authors":"Ali Akbar Ashkarran","doi":"10.1016/j.nantod.2025.102745","DOIUrl":"10.1016/j.nantod.2025.102745","url":null,"abstract":"<div><div>Protein/biomolecular corona (PC) is a layer of biomolecules (mainly proteins) that forms around the nanoparticles (NPs) after exposure to biological environments (e.g., blood). The dynamic nature of the PC formation allows enrichment of specific proteins particularly low abundance proteins and enables capturing disease-specific proteins on the surface of the NPs for biomarker discovery. However, identification of crucial proteins with high diagnostic values heavily depends on liquid chromatography mass spectrometry (LC-MS/MS) approach due to its sensitivity and specificity. Despite the widespread use of MS, there exist potential pitfalls in NPs’ PC analysis that may lead to misconduct in biomarker discovery studies. This opinion considers these pitfalls, providing insights into the challenges and strategies to mitigate misconduct in biomarker discovery using mass spectrometry techniques.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"62 ","pages":"Article 102745"},"PeriodicalIF":13.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-29DOI: 10.1016/j.nantod.2025.102739
Xiaoru Zhang , Shuiling Wen , Qin Liu , Wenli Cai , Keke Ning , Han Liu , Ergang Liu , Yongzhuo Huang , Feng Zeng
Diabetic wounds (DW) are characterized by excessive oxidative stress, chronic inflammation, hypoxia, impaired angiogenesis, weakened antioxidant defenses, and disrupted collagen remodeling, all of which delay healing and compromise tissue integrity. To address these challenges, we developed a biodegradable multifunctional hydrogel dressing (Fe/Ce@APS Gel) comprised of astragalus polysaccharide (APS), polyvinyl alcohol (PVA), and borax, functionalized with multi-enzyme mimetic nanozyme iron-modified ceria nanoparticles (Fe/CeNP-PEG). This Fe/Ce@APS Gel demonstrates potent anti-inflammatory, antioxidant, oxygenation, and pro-angiogenic properties, supporting wound healing across all stages. In the initial bleeding phase, the dressing accelerates blood clotting, promoting rapid wound stabilization. During the inflammatory phage, Fe/CeNP-PEG and APS effectively reduces excess reactive oxygen species (ROS) generates oxygen, modulates macrophage polarization, and mitigates inflammatory responses. In the proliferative phase, APS enhances cell proliferation, stimulates angiogenesis, and accelerates granulation tissue formation, supporting tissue repair. Finally, in the remodeling phase, Fe/Ce@APS Gel aids in tissue architecture reconstruction, strengthening wound integrity. Mechanistically, Fe/Ce@APS Gel facilitates DW healing by inhibiting the NLRP3/NF-κB signaling pathway, thereby reducing inflammation. The synergistic effects of APS and Fe/CeNP-PEG underscore the potential of Fe/Ce@APS Gel as a promising therapeutic dressing for DW treatment.
{"title":"Multi-functional nanozyme-integrated astragalus polysaccharide hydrogel for targeted phased therapy in diabetic wound healing","authors":"Xiaoru Zhang , Shuiling Wen , Qin Liu , Wenli Cai , Keke Ning , Han Liu , Ergang Liu , Yongzhuo Huang , Feng Zeng","doi":"10.1016/j.nantod.2025.102739","DOIUrl":"10.1016/j.nantod.2025.102739","url":null,"abstract":"<div><div>Diabetic wounds (DW) are characterized by excessive oxidative stress, chronic inflammation, hypoxia, impaired angiogenesis, weakened antioxidant defenses, and disrupted collagen remodeling, all of which delay healing and compromise tissue integrity. To address these challenges, we developed a biodegradable multifunctional hydrogel dressing (Fe/Ce@APS Gel) comprised of astragalus polysaccharide (APS), polyvinyl alcohol (PVA), and borax, functionalized with multi-enzyme mimetic nanozyme iron-modified ceria nanoparticles (Fe/CeNP-PEG). This Fe/Ce@APS Gel demonstrates potent anti-inflammatory, antioxidant, oxygenation, and pro-angiogenic properties, supporting wound healing across all stages. In the initial bleeding phase, the dressing accelerates blood clotting, promoting rapid wound stabilization. During the inflammatory phage, Fe/CeNP-PEG and APS effectively reduces excess reactive oxygen species (ROS) generates oxygen, modulates macrophage polarization, and mitigates inflammatory responses. In the proliferative phase, APS enhances cell proliferation, stimulates angiogenesis, and accelerates granulation tissue formation, supporting tissue repair. Finally, in the remodeling phase, Fe/Ce@APS Gel aids in tissue architecture reconstruction, strengthening wound integrity. Mechanistically, Fe/Ce@APS Gel facilitates DW healing by inhibiting the NLRP3/NF-κB signaling pathway, thereby reducing inflammation. The synergistic effects of APS and Fe/CeNP-PEG underscore the potential of Fe/Ce@APS Gel as a promising therapeutic dressing for DW treatment.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"62 ","pages":"Article 102739"},"PeriodicalIF":13.2,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-28DOI: 10.1016/j.nantod.2025.102742
Shengke Zhao , Yunjian Yu , Youtao Xin , Hegang Lu , Xiaohui Li , Shuyu Wang , Feihe Ma , Hui Gao
The gut microbiota plays a crucial role in host immune modulation and maintaining homeostasis. An abnormal increase in certain pathogens such as Fusobacterium nucleatum (Fn) can break homeostasis and drive the progression of various intestinal diseases. Supplementing probiotics can partially counteract these effects without flora disturbance. However, broad-spectrum antibacterial treatments are not compatible with probiotic therapy in a therapeutic system due to their non-selective damage on both probiotics and the overall gut microbiota. Herein, we screen and identify lauric acid (LA)-derived lipid, S12, from a combinatorial library of 12 chemically diverse lipids for its selective antibacterial activity against Fn over probiotic Clostridium butyricum (Cb). This lipid is then utilized as a single-cell carrier to orally deliver Cb (Cb@S12) for enhanced treatment of Fn-associated intestinal diseases. The surface arming of S12 effectively protects Cb from simulated gastric and intestinal fluids, thus significantly prolonging its intestinal retention in mice. Oral administration of Cb@S12 has demonstrated impressive therapeutic outcomes against Fn-aggravated inflammatory bowel disease and orthotopic colorectal cancer by selectively eliminating Fn while preserving the probiotic activity of Cb. This study introduces a robust approach using selectively antibacterial lipids for probiotic encapsulation, offering an antibiotic-free “probiotic-antagonistic” combination therapeutic strategy for intestinal diseases.
{"title":"Oral delivery of Clostridium butyricum using selective antibacterial lipids for enhanced treatment of Fusobacterium nucleatum-associated intestinal diseases","authors":"Shengke Zhao , Yunjian Yu , Youtao Xin , Hegang Lu , Xiaohui Li , Shuyu Wang , Feihe Ma , Hui Gao","doi":"10.1016/j.nantod.2025.102742","DOIUrl":"10.1016/j.nantod.2025.102742","url":null,"abstract":"<div><div>The gut microbiota plays a crucial role in host immune modulation and maintaining homeostasis. An abnormal increase in certain pathogens such as <em>Fusobacterium nucleatum</em> (<em>Fn</em>) can break homeostasis and drive the progression of various intestinal diseases. Supplementing probiotics can partially counteract these effects without flora disturbance. However, broad-spectrum antibacterial treatments are not compatible with probiotic therapy in a therapeutic system due to their non-selective damage on both probiotics and the overall gut microbiota. Herein, we screen and identify lauric acid (LA)-derived lipid, S12, from a combinatorial library of 12 chemically diverse lipids for its selective antibacterial activity against <em>Fn</em> over probiotic <em>Clostridium butyricum</em> (<em>Cb</em>). This lipid is then utilized as a single-cell carrier to orally deliver <em>Cb</em> (<em>Cb</em>@S12) for enhanced treatment of <em>Fn</em>-associated intestinal diseases. The surface arming of S12 effectively protects <em>Cb</em> from simulated gastric and intestinal fluids, thus significantly prolonging its intestinal retention in mice. Oral administration of <em>Cb</em>@S12 has demonstrated impressive therapeutic outcomes against <em>Fn</em>-aggravated inflammatory bowel disease and orthotopic colorectal cancer by selectively eliminating <em>Fn</em> while preserving the probiotic activity of <em>Cb</em>. This study introduces a robust approach using selectively antibacterial lipids for probiotic encapsulation, offering an antibiotic-free “probiotic-antagonistic” combination therapeutic strategy for intestinal diseases.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"62 ","pages":"Article 102742"},"PeriodicalIF":13.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-28DOI: 10.1016/j.nantod.2025.102730
Yang Cao , Yan Liu , Xiaoying Shang , Yao Lin , Lushan Lin , Ni Zhang , Hang Gao , Xueyuan Chen
The exploration of circularly polarized luminescence (CPL) materials based on perovskite quantum dots (PeQDs) has garnered significant interest across various disciplines owing to their extensive potential in optical applications. However, conventional perovskite-based CPL materials frequently encounter formidable challenges, including complex fabrication processes, limited emission bandwidths, inevitable anion exchange, and aggregation-induced quenching. To address these challenges, we proposed a unique approach to develop solid-state CPL nanohybrids with superior full-color CPL by integrating CsPbX3 (X = Cl, Br, I) PeQDs into amino acid co-assembled chiral metal-organic frameworks (CMOFs). By in situ generating PeQDs within L/D-ZIF-8 CMOFs, we achieved solid-state CPL nanohybrids (L/D-ZIF-8⊃PeQDs) that exhibited enhanced CPL properties and stability. The chiral microenvironment provided by the CMOFs not only boosts CPL performance but also effectively mitigates issues such as anion exchange and aggregation-induced quenching. More intriguingly, such nanohybrids displayed tunable CPL emissions across the entire visible spectrum, achieving a maximum dissymmetry factor (glum) value of 1.41 × 10−3 and a photoluminescence quantum yield of up to 13 %. Furthermore, we showcased their proof-of-concept application by fabricating circularly polarized red-green-blue and white light-emitting diodes with an impressive color gamut exceeding 137 % NTSC, thereby unveiling the significance of our approach in promoting CPL functionalities of perovskite-based materials.
{"title":"Full-color circularly polarized luminescence from perovskite quantum dots embedded within Chiral ZIF-8 matrix","authors":"Yang Cao , Yan Liu , Xiaoying Shang , Yao Lin , Lushan Lin , Ni Zhang , Hang Gao , Xueyuan Chen","doi":"10.1016/j.nantod.2025.102730","DOIUrl":"10.1016/j.nantod.2025.102730","url":null,"abstract":"<div><div>The exploration of circularly polarized luminescence (CPL) materials based on perovskite quantum dots (PeQDs) has garnered significant interest across various disciplines owing to their extensive potential in optical applications. However, conventional perovskite-based CPL materials frequently encounter formidable challenges, including complex fabrication processes, limited emission bandwidths, inevitable anion exchange, and aggregation-induced quenching. To address these challenges, we proposed a unique approach to develop solid-state CPL nanohybrids with superior full-color CPL by integrating CsPbX<sub>3</sub> (X = Cl, Br, I) PeQDs into amino acid co-assembled chiral metal-organic frameworks (CMOFs). By in situ generating PeQDs within L/D-ZIF-8 CMOFs, we achieved solid-state CPL nanohybrids (L/D-ZIF-8⊃PeQDs) that exhibited enhanced CPL properties and stability. The chiral microenvironment provided by the CMOFs not only boosts CPL performance but also effectively mitigates issues such as anion exchange and aggregation-induced quenching. More intriguingly, such nanohybrids displayed tunable CPL emissions across the entire visible spectrum, achieving a maximum dissymmetry factor (<em>g</em><sub>lum</sub>) value of 1.41 × 10<sup>−3</sup> and a photoluminescence quantum yield of up to 13 %. Furthermore, we showcased their proof-of-concept application by fabricating circularly polarized red-green-blue and white light-emitting diodes with an impressive color gamut exceeding 137 % NTSC, thereby unveiling the significance of our approach in promoting CPL functionalities of perovskite-based materials.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"62 ","pages":"Article 102730"},"PeriodicalIF":13.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143714656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Deubiquitinase (DUB)-based upstream regulation of inflammatory pathway has emerged as a promising strategy for inflammatory bowel disease (IBD) compared to clinical biological therapies that block downstream inflammatory mediators. Unfortunately, excessive reactive oxygen species (ROS) in the inflammatory microenvironment usually induces irreversible oxidation of the cysteine active-site of therapeutic DUB, resulting in the rapid fading of deubiquitylation activity and inferior anti-inflammatory efficacy. Herein, a redox active nanozyme boosted deubiquitylation strategy based on the natural-artificial dual catalytic nanodrug (NADCN) is designed for protecting the vulnerable catalytic active center of anti-inflammatory DUB. Benefiting from the nanozyme-enabled protection of DUB’s cysteine active-site, the NADCN exhibits far superior anti-inflammatory activity to that of its counterparts based on the conventional delivery system. Moreover, the redox nanozyme in NADCN confers efficient modulation of the oxidative and hypoxia inflammatory microenvironment, further unleashing the potential of DUB for treatment of IBD. Therefore, the NADCN opens a new avenue to protect the cysteine active-site of therapeutic DUB, providing an attractive opportunity for developing ROS-tolerant deubiquitylation therapy.
{"title":"Nanozyme engineered ROS-tolerant cysteine active-site for upstream deubiquitylation therapy of inflammatory bowel disease","authors":"Haibin Wu , Shuhan Shi , Lenan Xu , Ziying Zheng , Ziyan Huang , Yi Qiu , Jixiang Zhang , Keyun Yang , Yuting Xie , Cheng Xu , Tianyang Xu , Guohuan Zeng , Lingfeng Chen , Mincong Huang , Qian Chen , Daishun Ling , Guang Liang","doi":"10.1016/j.nantod.2025.102735","DOIUrl":"10.1016/j.nantod.2025.102735","url":null,"abstract":"<div><div>Deubiquitinase (DUB)-based upstream regulation of inflammatory pathway has emerged as a promising strategy for inflammatory bowel disease (IBD) compared to clinical biological therapies that block downstream inflammatory mediators. Unfortunately, excessive reactive oxygen species (ROS) in the inflammatory microenvironment usually induces irreversible oxidation of the cysteine active-site of therapeutic DUB, resulting in the rapid fading of deubiquitylation activity and inferior anti-inflammatory efficacy. Herein, a redox active nanozyme boosted deubiquitylation strategy based on the natural-artificial dual catalytic nanodrug (NADCN) is designed for protecting the vulnerable catalytic active center of anti-inflammatory DUB. Benefiting from the nanozyme-enabled protection of DUB’s cysteine active-site, the NADCN exhibits far superior anti-inflammatory activity to that of its counterparts based on the conventional delivery system. Moreover, the redox nanozyme in NADCN confers efficient modulation of the oxidative and hypoxia inflammatory microenvironment, further unleashing the potential of DUB for treatment of IBD. Therefore, the NADCN opens a new avenue to protect the cysteine active-site of therapeutic DUB, providing an attractive opportunity for developing ROS-tolerant deubiquitylation therapy.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"62 ","pages":"Article 102735"},"PeriodicalIF":13.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143714657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-26DOI: 10.1016/j.nantod.2025.102734
Hong Wang , Xuehe Lu , Jing Fan , Changping Yang , Hanyin Zhu , Jianbing Liu , Baoquan Ding
Drug delivery systems, based on chemical modification and controlled self-assembly of nucleic acid, have played an important role in the treatment of various diseases. Herein, we report a chemically conjugated DNA nanoplatform for efficient three-in-one tumor therapy in vivo. In our design, five copies of hydrophobic chemo-drug (camptothecin, CPT) are conjugated together by a branched organic molecule with an additional arm to introduce a DNA aptamer for targeting (Apt-5CPT). Meanwhile, a photosensitizer (HPPH, for photodynamic therapy) is efficiently organized at the terminal of an antisense oligonucleotide (AS, for gene therapy) to obtain another amphiphilic monomer (HPPH-AS). After co-assembly, a carrier-free DNA nanostructure, with the combination of chemotherapy, photodynamic therapy, and gene therapy, can be efficiently constructed for drug delivery. Under laser irradiation, the generated reactive oxygen species (ROS) can further facilitate their lysosomal escape to achieve subsequent glutathione (GSH)-based drug release for three-in-one tumor therapy in vivo. This rationally developed DNA nanoplatform-based drug delivery system presents a new avenue for the development of tumor therapy.
{"title":"A carrier-free DNA nanoplatform for efficient three-in-one tumor therapy in vivo","authors":"Hong Wang , Xuehe Lu , Jing Fan , Changping Yang , Hanyin Zhu , Jianbing Liu , Baoquan Ding","doi":"10.1016/j.nantod.2025.102734","DOIUrl":"10.1016/j.nantod.2025.102734","url":null,"abstract":"<div><div>Drug delivery systems, based on chemical modification and controlled self-assembly of nucleic acid, have played an important role in the treatment of various diseases. Herein, we report a chemically conjugated DNA nanoplatform for efficient three-in-one tumor therapy <em>in vivo</em>. In our design, five copies of hydrophobic chemo-drug (camptothecin, CPT) are conjugated together by a branched organic molecule with an additional arm to introduce a DNA aptamer for targeting (Apt-5CPT). Meanwhile, a photosensitizer (HPPH, for photodynamic therapy) is efficiently organized at the terminal of an antisense oligonucleotide (AS, for gene therapy) to obtain another amphiphilic monomer (HPPH-AS). After co-assembly, a carrier-free DNA nanostructure, with the combination of chemotherapy, photodynamic therapy, and gene therapy, can be efficiently constructed for drug delivery. Under laser irradiation, the generated reactive oxygen species (ROS) can further facilitate their lysosomal escape to achieve subsequent glutathione (GSH)-based drug release for three-in-one tumor therapy <em>in vivo</em>. This rationally developed DNA nanoplatform-based drug delivery system presents a new avenue for the development of tumor therapy.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"62 ","pages":"Article 102734"},"PeriodicalIF":13.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143714654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-26DOI: 10.1016/j.nantod.2025.102720
Shaowei Liu , Xue Li , Weifeng Wei , NaiJian Li , Ligeng Xu , Wei Huang , Yunxiang Zeng , Tianfeng Chen , Jinlin Wang
Malignant pleural mesothelioma (MPM) constitutes a rare classification of malignant tumors that originate within pleural tissue, with epithelioid tumors predominantly comprising its primary pathological subtype. Currently, the combination of pemetrexed and platinum remains the frontline therapeutic strategy for the treatment of MPM. However, recent advancements in the field of immune checkpoint inhibitors have redirected the research spotlight towards the intricate immune microenvironment of MPM. Selenium (Se), a vital trace element, plays pivotal roles in both antitumor and immunoregulation. This study delves into Se nanoparticles (SeNPs) and their functionalized derivatives, specifically lentinan-functionalized SeNPs (LET-SeNPs), with the aim of exploring their potential applications in the treatment of MPM. To tackle the challenge posed by pleural effusion (PE) in malignant pleural mesothelioma (MPM-PE), PE and peripheral blood samples were meticulously collected from MPM patients and subjected to processing utilizing LET-SeNPs. By evaluating the influence of low-energy transfer LET-SeNPs on lymphocytes, we observed an enhanced sensitivity of MPM to natural killer (NK) cells that were pretreated with LET-SeNPs. LET-SeNPs could activate NK92 cells in advance through the TrxR1-pSTAT3 pathway, and further enhance the toxic effect on MPM cells through the interaction of NKG2D-NKG2DL. This process has shown a powerful effect in reducing the invasiveness of MPM and enhancing its penetration and killing efficiency. This finding provides novel therapeutic insights and potential strategies for the treatment of patients with MPM.
{"title":"Translational selenium nanoparticles enhance NKG2D-mediated cytotoxicity of NK cells against malignant pleural mesothelioma cells through the TrxR1-pSTAT3 pathway","authors":"Shaowei Liu , Xue Li , Weifeng Wei , NaiJian Li , Ligeng Xu , Wei Huang , Yunxiang Zeng , Tianfeng Chen , Jinlin Wang","doi":"10.1016/j.nantod.2025.102720","DOIUrl":"10.1016/j.nantod.2025.102720","url":null,"abstract":"<div><div>Malignant pleural mesothelioma (MPM) constitutes a rare classification of malignant tumors that originate within pleural tissue, with epithelioid tumors predominantly comprising its primary pathological subtype. Currently, the combination of pemetrexed and platinum remains the frontline therapeutic strategy for the treatment of MPM. However, recent advancements in the field of immune checkpoint inhibitors have redirected the research spotlight towards the intricate immune microenvironment of MPM. Selenium (Se), a vital trace element, plays pivotal roles in both antitumor and immunoregulation. This study delves into Se nanoparticles (SeNPs) and their functionalized derivatives, specifically lentinan-functionalized SeNPs (LET-SeNPs), with the aim of exploring their potential applications in the treatment of MPM. To tackle the challenge posed by pleural effusion (PE) in malignant pleural mesothelioma (MPM-PE), PE and peripheral blood samples were meticulously collected from MPM patients and subjected to processing utilizing LET-SeNPs. By evaluating the influence of low-energy transfer LET-SeNPs on lymphocytes, we observed an enhanced sensitivity of MPM to natural killer (NK) cells that were pretreated with LET-SeNPs. LET-SeNPs could activate NK92 cells in advance through the TrxR1-pSTAT3 pathway, and further enhance the toxic effect on MPM cells through the interaction of NKG2D-NKG2DL. This process has shown a powerful effect in reducing the invasiveness of MPM and enhancing its penetration and killing efficiency. This finding provides novel therapeutic insights and potential strategies for the treatment of patients with MPM.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"62 ","pages":"Article 102720"},"PeriodicalIF":13.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143714655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-25DOI: 10.1016/j.nantod.2025.102733
Qianliao Zhou , Hongsu Wang , Lu Liu , Biao Dong , Liheng Sun , Xiaodi Niu
Antibacterial photodynamic therapy (aPDT) is an emerging and promising approach for addressing microbial contamination and antibiotic resistance. However, achieving efficient aPDT at reduced doses and under low light-intensity light remains a significant challenge. In this study, an aPDT strategy using solar irradiance intensity was proposed by designing a multifunctional antibacterial nanoplatform, denoted as YVO4:Bi3 +,Eu3+/TiO2-Ce6 (YTiC), based on rare-earth nanomaterials (YVO4:Bi3+,Eu3+) loaded with Ce6 and TiO2. The YVO4:Bi3+,Eu3+ serves as a carrier for Ce6 and TiO2, modulating aggregation state, optimizing light absorption, and suppressing electron-hole recombination in TiO2. The important innovation in this design is that YVO4:Bi3+,Eu3+ can convert UV light to red light to activate Ce6 and directly sensitize its triplet state via the 5D0–7F2 transition of Eu3+, thereby significantly boosting reactive oxygen species (ROS) production. This strategy reduces the required Ce6 dose by 46 % and light power density by 50 %, substantially enhancing ROS generation efficiency. Furthermore, combining aPDT with sonodynamic therapy (SDT) achieved near-complete bactericidal efficacy against Staphylococcus aureus, Salmonella typhimurium, and Botrytis cinerea, by inhibiting bacterial glycogen metabolism and disrupting the arginine and proline metabolism pathways. Based on this platform, the multifunctional antibacterial film YTiC-CMC is developed, enabling UV irradiation and sunlight to extend the shelf life of strawberries and chicken by 4 and 3 days, respectively, achieving outdoor antibacterial action and food preservation. This rare-earth nanomaterial-enhanced synergistic strategy offers a novel and powerful pathway for combating microbial contamination in both clinical applications and food preservation.
{"title":"Broad-spectrum utilization and direct energy transfer from lanthanide nanoparticles for sunlight-triggered low-dose, highly efficient photodynamic therapy","authors":"Qianliao Zhou , Hongsu Wang , Lu Liu , Biao Dong , Liheng Sun , Xiaodi Niu","doi":"10.1016/j.nantod.2025.102733","DOIUrl":"10.1016/j.nantod.2025.102733","url":null,"abstract":"<div><div>Antibacterial photodynamic therapy (aPDT) is an emerging and promising approach for addressing microbial contamination and antibiotic resistance. However, achieving efficient aPDT at reduced doses and under low light-intensity light remains a significant challenge. In this study, an aPDT strategy using solar irradiance intensity was proposed by designing a multifunctional antibacterial nanoplatform, denoted as YVO<sub>4</sub>:Bi<sup>3 +</sup>,Eu<sup>3+</sup>/TiO<sub>2</sub>-Ce6 (YTiC), based on rare-earth nanomaterials (YVO<sub>4</sub>:Bi<sup>3+</sup>,Eu<sup>3+</sup>) loaded with Ce6 and TiO<sub>2</sub>. The YVO<sub>4</sub>:Bi<sup>3+</sup>,Eu<sup>3+</sup> serves as a carrier for Ce6 and TiO<sub>2</sub>, modulating aggregation state, optimizing light absorption, and suppressing electron-hole recombination in TiO<sub>2</sub>. The important innovation in this design is that YVO<sub>4</sub>:Bi<sup>3+</sup>,Eu<sup>3+</sup> can convert UV light to red light to activate Ce6 and directly sensitize its triplet state via the <sup>5</sup>D<sub>0</sub>–<sup>7</sup>F<sub>2</sub> transition of Eu<sup>3+</sup>, thereby significantly boosting reactive oxygen species (ROS) production. This strategy reduces the required Ce6 dose by 46 % and light power density by 50 %, substantially enhancing ROS generation efficiency. Furthermore, combining aPDT with sonodynamic therapy (SDT) achieved near-complete bactericidal efficacy against <em>Staphylococcus aureus</em>, <em>Salmonella typhimurium</em>, and <em>Botrytis cinerea</em>, by inhibiting bacterial glycogen metabolism and disrupting the arginine and proline metabolism pathways. Based on this platform, the multifunctional antibacterial film YTiC-CMC is developed, enabling UV irradiation and sunlight to extend the shelf life of strawberries and chicken by 4 and 3 days, respectively, achieving outdoor antibacterial action and food preservation. This rare-earth nanomaterial-enhanced synergistic strategy offers a novel and powerful pathway for combating microbial contamination in both clinical applications and food preservation.</div></div>","PeriodicalId":395,"journal":{"name":"Nano Today","volume":"62 ","pages":"Article 102733"},"PeriodicalIF":13.2,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-25DOI: 10.1016/j.nantod.2025.102726
Zhaochu Xu , Jiang Yu , Yingxi Zhang , Yue Wang , Wenxiao Li , Baoyue Zhang , Wenwen Cui , Yicheng Li , Yue Wang , Zimeng Yang , Yubo Liu , Xin Li , Yongjun Wang , Zhonggui He , Hongzhuo Liu
Cancer vaccine has emerged as a promising therapeutic paradigm for cancer therapy. However, the lack of tumor-associated antigens and abundant immunosuppressive factors seriously diminish the efficacy of immunotherapy, resulting in poor clinical benefits. In this report, we engineered a coordinated immunostimulatory nanoplatform, termed MP@PPS NPs, by physically combining reactive oxygen species (ROS)-responsive poly (propylene sulfide) nanoparticles loaded with the photosensitizer pyropheophorbide a (PPa) and stimulator of interferon genes (STING) agonist (MSA-2), to function as an in situ cancer vaccine to amplify immunotherapeutic outcomes. Excellent stability of MP@PPS NPs endowed prolonged drug circulation time and improved tumor accumulation, while their small size boosted deeper drug penetration within tumors. Crucially, upon laser irradiation, the MP@PPS NPs could generate abundant ROS, which induced tumor ablation, triggered immunogenic cell death to initiate an adaptive antitumor immune response and facilitated the local release of MSA-2, thereby promoting innate antitumor immunity through the cGAS-STING pathway. MP@PPS NPs markedly suppressed both primary and distant tumor progression, promoted dendritic cell maturation and increased cytotoxic T lymphocyte infiltration, elicited robust antitumor immunity. Meanwhile, MP@PPS NPs treatment impeded the lung metastatic in conjunction with anti-PD-L1 treatment. This work holds significant promise for the synergistic photodynamic immunotherapy, and offers a crucial inspiration for addressing the problems of insufficient antitumor immunity and ineffective cancer treatments.
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