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Treatment of obliterated root canals using various guided endodontic techniques: a case series 使用各种引导根管技术治疗闭塞的根管:一个病例系列
IF 0.4 Q4 Dentistry Pub Date : 2020-06-04 DOI: 10.32067/GIE.2020.34.01.06
Antonietta Bordone, Cauris Cauvrechel
Objective: This article reports on 4 endodontic treatments of obliterated teeth using a guided endodontic (GE) technique. The aim is to demonstrate the benefits and limits of guided endodontics. Cases: The 4 patients consulted for endodontic treatment of an obliterated tooth. The teeth did not respond to pulp vitality tests. Retroalveolar X-rays and conebeam (CBCT) revealed the presence of a periapical lesion and of root canal obliteration. Patients’ permission was obtained to perform GE orthograde endodontic treatment. The first case was treated by GE using a closed guide and a metal sleeve. The second presented a more complex situation with the canal obliterated to the apical third of the tooth. Treatment of the third case involved the use of a closed guide without a metal sleeve. The fourth case was done using an open guide technique and the 2ingis system guiding the head of the contra-angle.     Treatment planning and guide manufacture were achieved by means of software programs initially designed for implantologists but which can also be used by endodontists. Root canal patency was restored in all patients. In each of the 4 cases, drilling was done using a small diameter (0.75 mm) cylindrical drill (FFDM Pneumat Tivoly). Once canal patency was restored using a C+ file, classic endodontic treatment could be performed.      Conclusion: GE assists endodontists in the management of complex cases by enabling centered drilling of the canal with no risk of deviating significantly from the virtually planned path. The novel choice of a small-diameter drill (0.75 mm) helps preserve the dental tissues.     Obiettivo: Questo articolo riporta 4 trattamenti endodontici di denti obliterati con una tecnica di endodonzia guidata (EG) L’obiettivo e di mettere in evidenza l’interesse e i limiti dell’endodonzia guidata. Casi: I 4 pazienti si sono presentati in appuntamento per il trattamento endodontico di un dente obliterato. I denti non rispondono ai test di vitalita. Le radiografie periapicali e la cbct mostrano la presenza di una lesione periapicale et di un’obliterazione canalare. Con l’accordo dei pazienti, e stato deciso di realizzare un trattamento endodontico ortogrado con EG. Il primo caso e stato trattato con EG con una dima “chiusa” e una boccola metallica. Il secondo caso e una situazione piu complessa di un canale obliterato fino al terzo apicale del dente. Il trattamento del terzo caso e stato realizzato con una dima “chiusa” senza boccola metallica. Il quarto caso e stato trattato con una tecnica a dima “aperta”. E stato  utilizzato un sistema 2Ingis che guida la testa del contrangolo. Le pianificazioni e la creazione delle dime sono state realizzate con dei programmi inizialmente dedicati all’implantologia ma che sono trasferibili all’endodonzia. La pervieta canalare e stata trovata in ciascuno dei casi. In ognuno dei casi la foratura e stata realizzata con una fresa cilindrica di piccolo diametro (0.75mm) (FFDM Pneumat Tivoly). Una
目的:报道4种牙根管引导治疗牙根管闭塞的方法。目的是证明引导牙髓学的优点和局限性。病例:4例患者就诊于牙髓治疗闭塞牙。牙齿对牙髓活力测试没有反应。牙槽后x线和锥束(CBCT)显示根尖周围病变和根管闭塞。在患者同意的情况下进行GE正畸根管治疗。第一个病例由GE使用封闭导管和金属套管进行治疗。第二个提出了一个更复杂的情况与根管湮没到尖牙的三分之一。第三个病例的治疗涉及使用没有金属套管的封闭导向器。第四例采用开放导向技术和2ingis系统引导对角头部。治疗计划和指南制作是通过最初为种植医生设计的软件程序来实现的,但也可以被牙髓医生使用。所有患者均恢复根管通畅。在4个案例中,每一个都使用小直径(0.75 mm)圆柱形钻头(FFDM Pneumat Tivoly)进行钻孔。一旦使用C+锉恢复根管通畅,就可以进行经典的根管治疗。结论:GE帮助牙髓医生管理复杂病例,使根管中心钻孔没有明显偏离虚拟计划路径的风险。新选择的小直径钻头(0.75毫米)有助于保护牙齿组织。目的:探讨牙髓病根治术治疗牙髓病的临床意义,探讨牙髓病治疗的局限性。目的:探讨牙髓病治疗中牙髓病的临床特点。我不认为我是在测试我的生命。根尖周围放射线摄影:根尖周围放射线摄影:根尖周围放射线摄影:根尖周围放射线摄影:根尖周围放射线摄影:根尖周围放射线摄影:根尖周围放射线摄影:根尖周围放射线摄影:根尖周围放射线摄影根据患者的病情,我们决定是否对牙根病进行治疗。首先,我想说的是,我们可以把它看作是一种金属,而不是一种金属。其次,我还将讨论一种情况,一种完全不可能的情况,一种完全不可能的情况,一种完全不可能的情况。我将对这一问题进行处理,以实现我对“chiusa”和“bococola metallica”的理解。我将四分之一的时间花在“技术上”和“自由”上。我们的国家利用了一个系统2,这是我们的指导方针。“植物化”、“植物化”、“自然化”、“自然化”、“自然化”、“自然化”、“自然化”、“自然化”、“自然化”、“自然化”、“自然化”。在casascuno dei casi中,La pervieta cananalare e stata trovata。在液压传动系统中,采用液压传动系统实现了直径为0.75mm (FFDM pneumatvoly)的液压传动系统。治疗牙髓病的方法:1 .牙髓病的治疗方法;2 .牙髓病的治疗方法。结论:牙周炎与牙周炎的关系为牙周炎与牙周炎的关系。在这一过程中,我们发现了一种特殊的现象,那就是,我们可以通过一种特殊的方式来实现对虚拟世界的理解。直径0.75 mm的牙尖牙尖牙是一种新型的牙尖牙保护装置。
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引用次数: 2
Effect of composite thickness over the fiber post on fracture resistance of endodontically treated teeth 纤维桩复合厚度对根管治疗后牙齿抗折性的影响
IF 0.4 Q4 Dentistry Pub Date : 2020-06-04 DOI: 10.32067/GIE.2020.34.01.11
Kasra Rahimipour, N. Panahandeh, S. Sheikh-Al-Eslamian, R. Mousavi, H. Torabzadeh
Aim: This study aimed to assess the effect of composite thickness over the fiber post on fracture resistance of endodontically treated teeth. Methods: This in vitro, experimental study was performed on 50 sound human premolars, which were randomly divided into 5 groups (n=10). Teeth in Group 1 remained intact while other specimens underwent root canal treatment with a Mesio-occluso-distal (MOD) cavity preparation, restored with fiber posts with a distance of 1.5 mm to the occlusal surface in groups 2 and 4 and 0.5 mm in groups 3 and 5. 24 hours of incubation was applied for groups 1-3 and 1 year of incubation for groups 4 and 5. Thereafter fracture strength measured using a Universal Testing Machine at a crosshead speed of 1 mm/minute. Data were analyzed using Shapiro-Wilk, Two-Way ANOVA and Tukey HSD. Results: The results showed a significant difference in terms of fracture resistance (P 0.05). Also, Tukey HSD revealed no statistically significant differences between Group 1 and 4 at one year. However, Group 1 (1255.25±280.61N) exhibited significantly higher fracture resistance than that of Group 5 (855.72±300.20N) at one year (P=0.027). The difference between other groups was not significant at any time point (P>0.05). Conclusions: By covering the fiber post with 1.5 mm thickness of composite resin and cuspal reduction of 2 mm, the fracture resistance of endodontically treated teeth can be increased to the level of sound teeth.
目的:研究纤维桩复合厚度对根管治疗后牙齿抗折性的影响。方法:选取50颗健康的人前磨牙进行体外实验研究,随机分为5组(n=10)。第1组牙齿保持完整,其余标本采用中牙合远端(meseo -occluso-distal, MOD)空腔制备根管治疗,第2、4组和第3、5组分别用距咬合面1.5 mm和0.5 mm的纤维桩修复。1 ~ 3组孵育24小时,4、5组孵育1年。然后用万能试验机以1毫米/分钟的十字速度测量断裂强度。采用Shapiro-Wilk、双向方差分析和Tukey HSD对数据进行分析。结果:两组在抗骨折性方面差异有统计学意义(p0.05)。此外,Tukey HSD在1年组1和4之间没有统计学上的显著差异。组1(1255.25±280.61N)的抗骨折能力明显高于组5(855.72±300.20N) (P=0.027)。各组间各时间点差异均无统计学意义(P>0.05)。结论:在纤维桩上覆盖1.5 mm厚度的复合树脂,牙尖复位2mm,可使根管治疗牙的抗折能力提高到健全牙的水平。
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引用次数: 1
Comparison of effectiveness of Reciproc, Wave One, Protaper, and One Shape rotary instruments in reduction of bacterial load in root canals Reciproc、Wave One、Protaper和One Shape旋转器械减少根管内细菌负荷的效果比较
IF 0.4 Q4 Dentistry Pub Date : 2020-06-04 DOI: 10.32067/GIE.2020.34.01.10
A. Azizi, Reza Hatam, P. Soltani, S. Miri, R. Abiri
Aim: To compare the effectiveness of Reciproc, Wave One, Protaper, and One Shape rotary instruments in reduction of E. faecalis in root canals. Methodology: In this in-vitro study, after initial stages of canal enlargement and irrigation, a suspension containing Entrococcus faecalis was inoculated into the root canals of 84 extracted single-canal premolars. The samples (apart from 2 positive and 2 negative controls) were randomly assigned into 4 groups according to rotary instruments used: Reciproc, Wave One, One Shape, Protaper. Each group was then subdivided to 2 groups based on irrigating solutions of normal saline and NaOCl. After instrumentation, the root canals were filled with brain-heart infusion (BHI) broth. Finally bacterial colony forming units (CFU) were counted. Results: Reduction in number of bacterial colonies before and after instrumentation and irrigation was not significantly different in different rotary instrument systems (P=0.128, F=1.955). However, NaOCl was more effective in reduction of bacterial load compared to normal saline (P<0.001, F=15.528). Conclusions: All rotary instruments used in the study are effective in reduction of the bacterial load.
目的:比较Reciproc、Wave One、Protaper和One Shape旋转器械在根管内减少粪肠杆菌的效果。方法:在这项体外研究中,在初始阶段的根管扩大和冲洗后,将含有粪Entrococcus faecalis的悬浮液接种到84颗拔出的单管前磨牙的根管中。样本(除2例阳性对照和2例阴性对照)根据使用的旋转器械随机分为4组:Reciproc、Wave One、One Shape、Protaper。各组再以生理盐水和NaOCl灌洗液为基础再分为2组。预备后,用脑-心灌注(BHI)肉汤填充根管。最后统计菌落形成单位(CFU)。结果:不同旋转式仪器系统对仪器冲洗前后菌落数量的减少差异无统计学意义(P=0.128, F=1.955)。然而,与生理盐水相比,NaOCl在减少细菌负荷方面更有效(P<0.001, F=15.528)。结论:本研究中使用的所有旋转器械均能有效降低细菌负荷。
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引用次数: 0
Diagnostic accuracy of two cone-beam computed tomography systems for detection of strip perforation in the mesial root of mandibular molars 两种锥束计算机断层扫描系统检测下颌磨牙近中根条状穿孔的诊断准确性
IF 0.4 Q4 Dentistry Pub Date : 2020-06-04 DOI: 10.32067/GIE.2020.34.01.01
Vida Maseratt, Heshmatallah Shahraki Ebrahimi, E. Saberi, Arezoo Pirhaji, Niloofar Khosravil
Aim: This study aimed to compare the diagnostic accuracy of two cone-beam computed tomography (CBCT) systems for detection of strip perforation in the mesiobuccal canal of mandibular molars after root canal treatment. Methodology: The curved mesiobuccal canals of mandibular first and second molars were instrumented as part of endodontic treatment. The canals were strip-perforated using #2 and #3 Gates-Glidden drills in distoaxial direction at 1 to 3 mm distance from the furcation. The canals were filled with gutta-percha and AH26 sealer with lateral compaction technique. The teeth were then mounted in dry bovine mandible and underwent CBCT using Acteon and NewTom CBCT systems. The CBCT scans were evaluated by two observers, and the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy of the two CBCT systems for detection of strip perforation were determined and compared. Results: The sensitivity and specificity for detection of strip perforation were 51.3% and 46.3% for Acteon, and 55% and 38.8% for NewTom CBCT system, respectively. The difference between the two CBCT systems for detection of strip perforation was not significant (Chi-square test, P>0.05). Conclusions: The accuracy of Acteon and NewTom CBCT systems for detection of strip perforation is low, and no significant difference was noted between the two systems in this respect.
目的:比较两种锥束计算机断层扫描(CBCT)系统对根管治疗后下颌磨牙中颊管带状穿孔的诊断准确性。方法:对下颌第一磨牙和第二磨牙弯曲的中颊根管进行根管治疗。使用2号和3号Gates-Glidden钻头在离分叉1 - 3mm处沿双轴方向进行条形穿孔。采用侧边压实技术,用杜仲胶和AH26密封胶填充根管。用Acteon和NewTom两种CBCT系统将牙固定在牛的干下颌骨上。两名观察员对CBCT扫描结果进行评估,确定并比较两种CBCT系统检测带状穿孔的敏感性、特异性、阳性预测值(PPV)、阴性预测值(NPV)和准确性。结果:Acteon检测条状穿孔的灵敏度和特异性分别为51.3%和46.3%,NewTom CBCT检测条状穿孔的灵敏度和特异性分别为55%和38.8%。两种CBCT系统对条带穿孔的检测差异无统计学意义(χ 2检验,P>0.05)。结论:Acteon和NewTom CBCT系统对条带穿孔的检测准确率较低,两种系统在这方面没有显著差异。
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引用次数: 1
Effects of mineral trioxide aggregate and platelet-rich fibrin on histological results of direct pulp capping in dogs 三氧化二矿聚集体和富血小板纤维蛋白对狗直接髓盖的组织学结果的影响
IF 0.4 Q4 Dentistry Pub Date : 2020-01-01 DOI: 10.32067/10.32067/GIE.2021.35.01.01
Bahareh Aghamohammadi Ameghani, A. Tavakoli, M. H. Tabatabaei, S. Valizadeh
Aim: This study aimed to compare the effects of mineral trioxide aggregate (MTA) and platelet-rich fibrin (PRF) on histological results of direct pulp capping (DPC) in dogs. Methodology: In this animal study, 36 class V cavities were prepared in the incisors of adult healthy mixed-breed dogs. The teeth were then randomly divided into three groups. No material was placed in the control teeth. MTA and PRF were placed on the exposure site in groups 2 and 3, respectively. After 2 months, the teeth were extracted under general anesthesia and were histological analyzed regarding inflammation, calcified bridge formation and necrosis. Data were analyzed using non-parametric Kruskal-Wallis test. Pairwise comparisons were made using the Mann-Whitney U test. Results: There are no statistically significant differences in terms of pulp inflammation, dentinal bridge formation and necrosis among the treatment groups capping with MTA and PRF. (P Value >0.05), however, PRF and MTA were the same in all parameters, these groups were both significantly superior to the control group. Conclusion: Within the limitations of this study, PRF can be used for DPC as an alternative to MTA.
目的:比较三氧化矿物聚集体(MTA)和富血小板纤维蛋白(PRF)对狗直接髓盖(DPC)组织学结果的影响。方法:本动物实验在健康成年杂交犬的门牙上制备了36个V类空腔。然后这些牙齿被随机分成三组。对照牙内未放置任何材料。2组和3组分别在暴露部位放置MTA和PRF。2个月后全麻拔牙,观察组织炎症、钙化桥形成及坏死情况。数据分析采用非参数Kruskal-Wallis检验。两两比较采用Mann-Whitney U检验。结果:MTA盖顶组与PRF盖顶组在牙髓炎症、牙本质桥形成和坏死方面差异无统计学意义。(P值>0.05),但各参数PRF、MTA均无显著差异,且均显著优于对照组。结论:在本研究的局限性内,PRF可以作为MTA的替代方法用于DPC。
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引用次数: 1
Current applications of lasers in endodontics 激光在牙髓学中的应用现状
IF 0.4 Q4 Dentistry Pub Date : 2019-10-31 DOI: 10.32067/GIE.2019.33.02.01
Maryam Kuzekanani, G. Plotino, J. Gutmann
The use of lasers within the scope of endodontic practice and research has increased significantly in the past few years. Lasers are generally classified according to their physical constructions and special wavelengths, which have impacts on their enhanced clinical usage. Lasers according to their physical constructions are divided into three groups. The first type is the solid state laser, so named because the medium, undergoing lasing, is in a solid form. Ruby laser is the prototype of all solid state lasers. By forming crystalline materials that are doped with rare earth elements a wide range of solid state lasers can be produced. Some of the most common types of solid state lasers use the YAG (Yttrium Aluminium Garnet) crystal (Holmium:YAG, Thulium:YAG Neodymium:YAG and Erbium:YAG) and the YSGG (Yttrium Scandium Gallium Garnet) crystal (Er,Cr:YSGG) as their base. The second major family of lasers are the gas lasers. In this group the lasing material that is ionized can be Argon gas, Carbon dioxide gas, Nitrogen gas or a Helium-Neon (He:Ne) gas mixture. The third family of lasers are the Diode lasers, which produce wavelengths in the visible spectrum. The most frequently used lasers in endodontics are: Neodymium:YAG (Nd:YAG), Diode Laser, Erbium:YAG (Er:YAG), Erbium Chromium:YSGG (Er,Cr:YSGG) and He:Ne laser. This paper reviews the most common applications of lasers in endodontics that include Laser Doppler Flowmetry (LDF), treatment of dentinal hypersensitivity, pulpotomy and pulp capping and root canal disinfection through laser activated irrigation and photo-activated root canal disinfection (PAD).
在过去的几年里,在牙髓治疗的实践和研究中,激光的使用显著增加。激光通常根据其物理结构和特殊波长进行分类,这对其增强临床应用有影响。激光器根据其物理结构分为三组。第一种是固态激光器,之所以这样命名是因为进行激光的介质是固态的。红宝石激光器是所有固态激光器的原型。通过形成掺杂稀土元素的晶体材料,可以生产各种固态激光器。一些最常见的固态激光器使用YAG(钇铝石榴石)晶体(钬:YAG,铥:YAG钕:YAG和铒:YAG)和YSGG(钇钪镓石榴石)晶体(Er,Cr:YSGG)作为它们的基础。第二大类激光器是气体激光器。在这一组中,被电离的激光材料可以是氩气、二氧化碳气体、氮气或氦氖(He:Ne)气体混合物。第三类激光器是二极管激光器,它产生可见光谱中的波长。牙髓治疗中最常用的激光有:钕钇铝石榴石(Nd:YAG)、二极管激光、铒钇石榴石(Er:YAG)、铒铬钇石榴石(Er,Cr:YSGG)和He:Ne激光。本文综述了激光在牙髓学中最常见的应用,包括激光多普勒血流测量(LDF)、牙本质过敏的治疗、牙髓切开术和牙髓盖盖以及通过激光激活冲洗和光激活根管消毒(PAD)进行根管消毒。
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引用次数: 5
A mandibular molar with four independent mesial roots: a case report 下颌磨牙具有四个独立的近中根:1例报告
IF 0.4 Q4 Dentistry Pub Date : 2019-10-31 DOI: 10.32067/GIE.2019.33.02.02
C. Maniglia-Ferreira, F. Almeida-Gomes, Eduardo Diogo Gurgel Filho, Natalia Santiago, F. G. Pappen
Aim: The most common anatomical configuration of the mandibular molar is to present two roots and three or four canals, but it’s possible to find many different configurations. A case of unusual mandibular molar is presented to illustrate the anatomic root canal variation. Summary: Endodontic treatment was performed in a mandibular third molar with five roots, four in the mesial portion and one in the distal portion. The x-ray examination showed an abnormal root canal anatomy, suggesting the presence of extra mesial roots. Cone-beam computed tomography (CBCT) imaging revealed five roots and five root canals, with four independent roots in the mesial portion and one in the distal portion, indicating a rare anatomic configuration. This case report presents the importance of searching for extra canals and the unusual canal morphology, because the knowledge of the most common anatomic characteristics and their possible variations is fundamental to the endodontic treatment success. Key learning points: (A) The major cause of failure in root canal therapy is the inability to localize and treat all of the canals of the root canal system. (B) Mandibular molars may have complex canal systems and variations. (C) The operating microscope and CBCT interpretation are fundamental in confirming and preventing mistakes about the configuration of root canals.
目的:下颌磨牙最常见的解剖结构是有两个根和三个或四个管,但也有可能发现许多不同的结构。一个不寻常的下颌磨牙的情况提出,以说明解剖根管变异。摘要:对下颌第三磨牙进行了根管治疗,其中有五个根,四个在中端,一个在远端。x线检查显示根管解剖异常,提示存在外内侧根。锥形束计算机断层扫描(CBCT)成像显示5根和5根管,其中4根在中端,1根在远端,显示一种罕见的解剖构型。本病例报告提出了寻找额外的根管和不寻常的根管形态的重要性,因为了解最常见的解剖特征及其可能的变化是根管治疗成功的基础。主要学习要点:(A)根管治疗失败的主要原因是无法定位和治疗根管系统的所有管。(B)下颌磨牙可能有复杂的管系和变异。(C)手术显微镜和CBCT解释是确认和防止根管形态错误的基础。
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引用次数: 0
Ni-Ti alloy remnants after root canal preparation with Ni-Ti engine-driven files: a preliminary report 镍钛发动机驱动锉预备根管后镍钛合金残余:初步报告
IF 0.4 Q4 Dentistry Pub Date : 2019-10-31 DOI: 10.32067/GIE.2019.33.02.05
S. Stefanescu, M. Popescu, L. Tudoran, Haider Al-Saffar
Aim: Aim of the present study was to assess any signs of Ni-Ti alloy remnants in the root canal after preparation and investigate if this aspect may be influenced by the steam sterilization of the files. Materials and Methods: 20 extracted upper first premolars with complete root formation and apical foramina and three kits of BioRace instruments (FKG, Le Chaux-of-Fonds, Switzerland) were used in the present study. The first BioRace kit was used to prepare 10 teeth (20 canals), without sterilization between the root canal preparations, while the second sample group was subjected to steam sterilization after each tooth (two canals) prepared. The third kit of instruments was used as a control group. Files were then analyzed by means of scanning electron microscope (SEM) using magnifications between 500 and 4.000x. Images were taken on sections D1-D8 of each file. Files weight was measured using a precision weight scale with readability of 0,001g to quantify the possible alloy loss and file degradation occurred during their use. Teeth were then sectioned on their length and submitted to EDX analysis in search of possible alloy particles within the root canals, using magnifications between 30 and 300x. In the same manner, the irrigant used during the root shaping was collected and analyzed. Differences in weight were statistically analysed using the independent sample Student t-test (p<0.05). Results: No file separation was registered in both groups analyzed. Weight measurements and SEM micrographs showed signs of higher degradation for samples from group 2. EDX analysis showed some Ni-Ti particles in roots prepared in group 2. Conclusions: Metal strips, weight loss and micro-fractures appeared on the files subjected to steam sterilization between the uses. Ni-Ti particles detached from files, during their intra-canal use were found on the root canal walls.
目的:本研究的目的是评估制备后根管中是否有镍钛合金残留的迹象,并探讨蒸汽灭菌对这方面的影响。材料与方法:本研究使用20颗拔牙的上颌第一前磨牙,牙根形成完整,有尖孔,3套BioRace仪器(FKG, Le Chaux-of-Fonds,瑞士)。第一组使用BioRace试剂盒制备10颗牙(20根管),两次根管制备之间不进行灭菌,第二组在每颗牙(2根管)制备后进行蒸汽灭菌。第三套仪器作为对照组。然后通过扫描电子显微镜(SEM)分析文件,使用500到4.000倍的放大倍数。在每个文件的D1-D8切片上拍照。锉刀的重量是用精度重量秤测量的,可读性为0.001 g,以量化在使用过程中可能发生的合金损失和锉刀退化。然后根据牙齿的长度对其进行切片,并提交给EDX分析,使用30到300倍的放大倍数来寻找根管内可能的合金颗粒。以同样的方式,收集和分析了根成型过程中使用的灌溉剂。体重差异采用独立样本Student t检验进行统计学分析(p<0.05)。结果:两组均未见文件分离。重量测量和SEM显微照片显示,2组样品的降解程度更高。EDX分析显示,2组制备的根中有Ni-Ti颗粒。结论:蒸汽灭菌锉在使用间隙出现金属条、重量减轻和微断裂现象。在根管内使用时,在根管壁上发现镍钛颗粒脱落。
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引用次数: 1
Effect of Emdogain coated endodontic materials on viability of human dental pulp stem cells (HDPSCs) Emdogain包被根管材料对人牙髓干细胞(HDPSCs)活力的影响
IF 0.4 Q4 Dentistry Pub Date : 2019-10-31 DOI: 10.32067/GIE.2019.33.02.04
Hamed Karkehabadi, Shahriar Shahriari, R. Najafi, E. Khoshbin, H. Abbaspourrokni, Z. Pakseresht
Aim: Biocompatibility is an important characteristic of dental pulp capping agents. This study aimed to assess the effect of mineral trioxide aggregate (MTA), calcium enriched mixture (CEM) cement and Biodentine with/without Emdogain (EMD) on the viability of human dental pulp stem cells (HDPSCs). Methodology: In this in vitro study, HDPSCs were isolated from the root canal of an extracted impacted third molar tooth and cultured. The cells were exposed to freshly prepared endodontic cements in six groups of MTA, MTA plus EMD, CEM cement, CEM cement plus EMD, Biodentine and Biodentine plus EMD in 24-well plates for 24, 48 and 168 hours (6 wells/repetitions for each material group at each time point). Cell viability was evaluated at each time point using the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. Results: Cell viability was not significantly different at different time points in any endodontic cement group (p>0.05) except for CEM cement/EMD group (p=0.00). At 24 hours, MTA/EMD and MTA showed the highest cell viability (p=0.001). Similar results were obtained at 48 hours (p=0.000). At 168 hours, MTA/EMD and CEM cement/ EMD showed the highest cell viability (p=0.000). Addition of EMD had no significant effect on cell viability in any cement group at 24 or 48 hours. However, addition of EMD to MTA and CEM cement increased the viability of HDPSCs at 168 hours. Conclusions: Addition of EMD to MTA and CEM cement can increase the viability of HDPSCs at 7 days. © 2019 Masson SpA. All rights reserved.
目的:生物相容性是牙髓封盖剂的重要特性。本研究旨在评估三氧化矿物骨料(MTA)、富钙合剂(CEM)和生物牙乳清(EMD)对人牙髓干细胞(HDPSCs)活力的影响。方法:在这项体外研究中,从拔出的阻生第三磨牙的根管中分离出hdpsc并进行培养。将细胞分为MTA、MTA + EMD、CEM水泥、CEM水泥+ EMD、Biodentine和Biodentine + EMD六组,分别暴露于24孔板中24、48和168小时(每个时间点每个材料组6孔/重复)。使用甲基噻唑四氮唑(MTT)法评估每个时间点的细胞活力。使用Kruskal-Wallis和Mann-Whitney检验分析数据。结果:除CEM + EMD组外,各组间细胞活力在不同时间点差异均无统计学意义(p>0.05)。24小时时,MTA/EMD和MTA的细胞活力最高(p=0.001)。在48小时时获得了类似的结果(p=0.000)。168 h时,MTA/EMD和CEM水泥/EMD的细胞活力最高(p=0.000)。添加EMD对任何水泥组24或48小时的细胞活力均无显著影响。然而,在MTA和CEM水泥中加入EMD可以提高hdpsc在168小时的存活率。结论:在MTA和CEM水泥中添加EMD可提高hdpsc在7天的存活率。©2019 Masson SpA。版权所有。
{"title":"Effect of Emdogain coated endodontic materials on viability of human dental pulp stem cells (HDPSCs)","authors":"Hamed Karkehabadi, Shahriar Shahriari, R. Najafi, E. Khoshbin, H. Abbaspourrokni, Z. Pakseresht","doi":"10.32067/GIE.2019.33.02.04","DOIUrl":"https://doi.org/10.32067/GIE.2019.33.02.04","url":null,"abstract":"Aim: Biocompatibility is an important characteristic of dental pulp capping agents. This study aimed to assess the effect of mineral trioxide aggregate (MTA), calcium enriched mixture (CEM) cement and Biodentine with/without Emdogain (EMD) on the viability of human dental pulp stem cells (HDPSCs). Methodology: In this in vitro study, HDPSCs were isolated from the root canal of an extracted impacted third molar tooth and cultured. The cells were exposed to freshly prepared endodontic cements in six groups of MTA, MTA plus EMD, CEM cement, CEM cement plus EMD, Biodentine and Biodentine plus EMD in 24-well plates for 24, 48 and 168 hours (6 wells/repetitions for each material group at each time point). Cell viability was evaluated at each time point using the methyl thiazolyl tetrazolium (MTT) assay. Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. Results: Cell viability was not significantly different at different time points in any endodontic cement group (p>0.05) except for CEM cement/EMD group (p=0.00). At 24 hours, MTA/EMD and MTA showed the highest cell viability (p=0.001). Similar results were obtained at 48 hours (p=0.000). At 168 hours, MTA/EMD and CEM cement/ EMD showed the highest cell viability (p=0.000). Addition of EMD had no significant effect on cell viability in any cement group at 24 or 48 hours. However, addition of EMD to MTA and CEM cement increased the viability of HDPSCs at 168 hours. Conclusions: Addition of EMD to MTA and CEM cement can increase the viability of HDPSCs at 7 days. © 2019 Masson SpA. All rights reserved.","PeriodicalId":42221,"journal":{"name":"Giornale Italiano di Endodonzia","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73779248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Effect of chlorhexidine and isopropyl alcohol on immediate and delayed bond strength of glass fiber posts 氯己定和异丙醇对玻璃纤维桩的即时和延迟粘结强度的影响
IF 0.4 Q4 Dentistry Pub Date : 2019-10-31 DOI: 10.32067/GIE.2019.33.02.06
Ellizandra Anater Lecardelli, V. Poletto-Neto, F. G. Pappen, T. Pereira-Cenci, R. D. Morgental
Aim: To investigate the effects of dentinal treatment with chlorhexidine and isopropyl alcohol, alone or associated, on the bond strength and bond stability of glass fiber posts cemented with a self-adhesive resin cement. Materials and Methods: Forty bovine teeth were endodontically treated and randomly distributed into four groups, according to the dentinal treatment after post space preparation: distilled water (Control); 2% chlorhexidine (CHX); isopropyl alcohol (ISO); 2% chlorhexidine + isopropyl alcohol (CHX+ISO). Glass fiber posts were cemented with a self-adhesive resin cement and after 24 h specimens were cut into six 1.5 mm-thick slices. One slice of each root third (i.e. apical, middle and coronal) was subjected to immediate push-out test and the other was stored in distilled water at 37 °C for six months for delayed push-out test. Bond strength was registered in megapascal (MPa). Failure mode was evaluated by a stereomicroscope, with 40x magnification. Data analysis was performed using Kruskal-Wallis and Friedman tests, with 5% significance level. Results: No significant differences were detected between experimental groups or between root thirds in the same group in the immediate evaluation. After six months of aging, all groups showed a significant decrease in bond strength values, but ISO group presented higher bond strength than Control and CHX groups (p=0.0001). The most common type of failure for all groups was adhesive between resin cement and root dentine. Conclusions: Dentinal treatment with CHX and ISO, isolated or combined, did not affect the immediate bond strength of glass fiber posts, but the ISO group presented better bond stability after six-month aging.
目的:探讨氯己定和异丙醇单独或联合治疗对自粘树脂水泥固接玻璃纤维桩的粘结强度和粘结稳定性的影响。材料与方法:对40颗牛牙进行根管治疗,根据牙槽位准备后的处理情况随机分为4组:蒸馏水组(对照组);2%氯己定(CHX);异丙醇;2%氯己定+异丙醇(CHX+ISO)。玻璃纤维桩用自粘树脂水泥胶结,24h后将试件切成6片1.5 mm厚的薄片。每三分之一根(即根尖、中间和冠状)的一片进行立即推出试验,另一片在37°C蒸馏水中保存6个月进行延迟推出试验。粘结强度单位为兆帕斯卡(MPa)。通过40倍放大的体视显微镜评估失效模式。数据分析采用Kruskal-Wallis和Friedman检验,显著性水平为5%。结果:实验组间、同一组三分根间即刻评价无显著差异。陈化6个月后,各组结合力值均显著降低,但ISO组结合力值高于对照组和CHX组(p=0.0001)。所有组中最常见的失败类型是树脂水泥与牙根本质之间的粘接。结论:CHX与ISO单独或联合处理牙本质均不影响玻璃纤维桩的即时粘结强度,但ISO组在6个月老化后具有更好的粘结稳定性。
{"title":"Effect of chlorhexidine and isopropyl alcohol on immediate and delayed bond strength of glass fiber posts","authors":"Ellizandra Anater Lecardelli, V. Poletto-Neto, F. G. Pappen, T. Pereira-Cenci, R. D. Morgental","doi":"10.32067/GIE.2019.33.02.06","DOIUrl":"https://doi.org/10.32067/GIE.2019.33.02.06","url":null,"abstract":"Aim: To investigate the effects of dentinal treatment with chlorhexidine and isopropyl alcohol, alone or associated, on the bond strength and bond stability of glass fiber posts cemented with a self-adhesive resin cement. \u0000Materials and Methods: Forty bovine teeth were endodontically treated and randomly distributed into four groups, according to the dentinal treatment after post space preparation: distilled water (Control); 2% chlorhexidine (CHX); isopropyl alcohol (ISO); 2% chlorhexidine + isopropyl alcohol (CHX+ISO). Glass fiber posts were cemented with a self-adhesive resin cement and after 24 h specimens were cut into six 1.5 mm-thick slices. One slice of each root third (i.e. apical, middle and coronal) was subjected to immediate push-out test and the other was stored in distilled water at 37 °C for six months for delayed push-out test. Bond strength was registered in megapascal (MPa). Failure mode was evaluated by a stereomicroscope, with 40x magnification. Data analysis was performed using Kruskal-Wallis and Friedman tests, with 5% significance level. Results: No significant differences were detected between experimental groups or between root thirds in the same group in the immediate evaluation. After six months of aging, all groups showed a significant decrease in bond strength values, but ISO group presented higher bond strength than Control and CHX groups (p=0.0001). The most common type of failure for all groups was adhesive between resin cement and root dentine. \u0000Conclusions: Dentinal treatment with CHX and ISO, isolated or combined, did not affect the immediate bond strength of glass fiber posts, but the ISO group presented better bond stability after six-month aging.","PeriodicalId":42221,"journal":{"name":"Giornale Italiano di Endodonzia","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2019-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78115178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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Giornale Italiano di Endodonzia
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