Sensing and Bio-Sensing Research最新文献_第3页

One-pot green synthesis of BSA-capped O-CQDs as an effective fluorescent sensing platform for sensitive and selective detection of promethazine drug

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100756

S. Viji , A. Dinesh , K. Radhakrishnan , L. Srimathi Priya , C. Sivasankari , Madhappan Santhamoorthy , Manikandan Ayyar , V. Mohanavel , Mohamed Hashem , Hassan Fouad , G. Ramachandran , S. Santhoshkumar

Reliability in biosensing technologies is crucial for the accurate detection and measurement of target molecules, prompting strategies to minimise non-specific binding events that may result in inaccurate findings. This work reports on bovine serum albumin (BSA)-capped oxygen rich carbon quantum dots (O-CQDs) to improve the accuracy of promethazine sensing. The CQDs were produced utilizing a hydrothermal technique with apple juice as a sustainable carbon source. BSA-Caped O-CQDs improve their biocompatibility and stability in physiological conditions while also reducing non-specific interactions with other biomolecules. Several spectroscopic methods, such as fluorescence, X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR), were used to evaluate this enhanced specificity and sensitivity. Obtained results show that BSA-O-CQDs had higher specificity for promethazine Furthermore, their ability to resolve non-specific binding was assessed under simulated physiological backgrounds, demonstrating their promise for robust biosensing applications. This sensor method achieved a high selectivity with LOD of 0.2 μM from range of 10–90 μM indicating its suitability for precise promethazine measurement in environmental samples. The primary advantages of this technique include simplified sample preparation together with economic advantages which make it a critical instrument for public health monitoring as well as cutting-edge pharmaceutical research. Sustainable O-CQDs demonstrate the potential for creating environmentally friendly biosensors.

{"title":"One-pot green synthesis of BSA-capped O-CQDs as an effective fluorescent sensing platform for sensitive and selective detection of promethazine drug","authors":"S. Viji , A. Dinesh , K. Radhakrishnan , L. Srimathi Priya , C. Sivasankari , Madhappan Santhamoorthy , Manikandan Ayyar , V. Mohanavel , Mohamed Hashem , Hassan Fouad , G. Ramachandran , S. Santhoshkumar","doi":"10.1016/j.sbsr.2025.100756","DOIUrl":"10.1016/j.sbsr.2025.100756","url":null,"abstract":"<div><div>Reliability in biosensing technologies is crucial for the accurate detection and measurement of target molecules, prompting strategies to minimise non-specific binding events that may result in inaccurate findings. This work reports on bovine serum albumin (BSA)-capped oxygen rich carbon quantum dots (O-CQDs) to improve the accuracy of promethazine sensing. The CQDs were produced utilizing a hydrothermal technique with apple juice as a sustainable carbon source. BSA-Caped O-CQDs improve their biocompatibility and stability in physiological conditions while also reducing non-specific interactions with other biomolecules. Several spectroscopic methods, such as fluorescence, X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR), were used to evaluate this enhanced specificity and sensitivity. Obtained results show that BSA-O-CQDs had higher specificity for promethazine Furthermore, their ability to resolve non-specific binding was assessed under simulated physiological backgrounds, demonstrating their promise for robust biosensing applications. This sensor method achieved a high selectivity with LOD of 0.2 μM from range of 10–90 μM indicating its suitability for precise promethazine measurement in environmental samples. The primary advantages of this technique include simplified sample preparation together with economic advantages which make it a critical instrument for public health monitoring as well as cutting-edge pharmaceutical research. Sustainable O-CQDs demonstrate the potential for creating environmentally friendly biosensors.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100756"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress and application prospects of flexible wearable sensor in spacesuit

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100750

Aiming Bu , Wanxin Zhang , Qian Ran , Dongyue Liu , Jialu Ma , Rongqing Wang , Kun Shang , Hongrui Yang , Zhiqiang Mei

Spacesuit provide the basic life support for astronauts in the space environment. Astronauts in extravehicular operations need spacesuit for pressure to form a vacuum protection. However the suit pressure caused greater joint activity resistance impact on the operation of astronauts. The wearable sensors have drawn more and more attention because its operations are simple and real-time detections are available. It can realize the benefits of heart rate, breathing, sweat, action recognition and date acquisition, etc. The joint resistance characteristics of spacesuit is revealed under pressure were studied by wearing flexible wearable sensors, which provides a theoretical basis for improving the performance of spacesuit. Based on the above problems, this paper summarizes the latest research progress of wearable flexible sensor category and application of different types of flexible sensors. Finally, the challenges in the application of flexible wearable sensors in the spacesuit were discussed. It provides a theoretical basis for the research in related fields.

{"title":"Research progress and application prospects of flexible wearable sensor in spacesuit","authors":"Aiming Bu , Wanxin Zhang , Qian Ran , Dongyue Liu , Jialu Ma , Rongqing Wang , Kun Shang , Hongrui Yang , Zhiqiang Mei","doi":"10.1016/j.sbsr.2025.100750","DOIUrl":"10.1016/j.sbsr.2025.100750","url":null,"abstract":"<div><div>Spacesuit provide the basic life support for astronauts in the space environment. Astronauts in extravehicular operations need spacesuit for pressure to form a vacuum protection. However the suit pressure caused greater joint activity resistance impact on the operation of astronauts. The wearable sensors have drawn more and more attention because its operations are simple and real-time detections are available. It can realize the benefits of heart rate, breathing, sweat, action recognition and date acquisition, etc. The joint resistance characteristics of spacesuit is revealed under pressure were studied by wearing flexible wearable sensors, which provides a theoretical basis for improving the performance of spacesuit. Based on the above problems, this paper summarizes the latest research progress of wearable flexible sensor category and application of different types of flexible sensors. Finally, the challenges in the application of flexible wearable sensors in the spacesuit were discussed. It provides a theoretical basis for the research in related fields.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100750"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143349032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecularly imprinted polymers-based electrochemical sensors for tracking vitamin B12 released from spray-dried microcapsules during in vitro simulated gastrointestinal digestion

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100759

Seyed Mohammad Taghi Gharibzahedi , Gauri Kishore Hasabnis , Eda Akin , Zeynep Altintas

Highly sensitive detection of vitamin B₁₂ (VB₁₂) is crucial for assessing its bioavailability and diagnosing deficiency-related disorders. VB₁₂ with 87.69 % efficiency was microencapsulated by spray drying complex coacervation-based emulsions of Arabic gum and sonicated insect protein, forming 5.4 μm semi-spherical microcapsules with smooth or wrinkled surfaces. The optimized VB₁₂-specific o-aminophenol (AP)-based molecularly imprinted polymer (MIP) film demonstrated efficient molecular recognition and significant electrochemical responsiveness, enabling VB₁₂ detection in PBS buffer with an LOD of 0.5 μM within a linear range of 0.5–10 μM (R² = 0.991). Surface characterization revealed remarkable property changes during sensor fabrication. A significant reduction in the hydrophilicity of the VB₁₂-AP-MIP film after template removal (TR) was observed due to the increased contact angle from 33.1° to 55.82° (p < 0.05). Morphological analysis using atomic force microscopy (AFM) showed the highest roughness (90.57 nm) for the VB₁₂-AP-MIP film, decreasing after TR (30.56 nm) and increasing upon VB₁₂ capture (46.04 nm). Scanning electron microscopy (SEM) revealed smoother, more uniform surfaces for MIP films than non-imprinted polymer (NIP) films, indicating the template's critical role in polymerization. The developed electro-MIP sensor exhibited good template-selectivity and a 15-day storage stability at 4 °C. The electrochemical VB₁₂-AP-MIP sensor successfully detected VB₁₂ in digested microcapsules under simulated salivary (3.30 ± 0.46 μM), gastric (64.87 ± 2.66 μM), and intestinal (76.68 ± 2.16 μM) conditions. The VB₁₂ analysis using HPLC and UV–Vis spectrophotometry showed that the electro-MIP sensor outcomes were comparable to those obtained with HPLC. The developed electro-MIP sensor would be a promising tool for determining VB₁₂ levels in complex biological samples.

{"title":"Molecularly imprinted polymers-based electrochemical sensors for tracking vitamin B12 released from spray-dried microcapsules during in vitro simulated gastrointestinal digestion","authors":"Seyed Mohammad Taghi Gharibzahedi , Gauri Kishore Hasabnis , Eda Akin , Zeynep Altintas","doi":"10.1016/j.sbsr.2025.100759","DOIUrl":"10.1016/j.sbsr.2025.100759","url":null,"abstract":"<div><div>Highly sensitive detection of vitamin B<sub>12</sub> (VB<sub>12</sub>) is crucial for assessing its bioavailability and diagnosing deficiency-related disorders. VB<sub>12</sub> with 87.69 % efficiency was microencapsulated by spray drying complex coacervation-based emulsions of Arabic gum and sonicated insect protein, forming 5.4 μm semi-spherical microcapsules with smooth or wrinkled surfaces. The optimized VB<sub>12</sub>-specific o-aminophenol (AP)-based molecularly imprinted polymer (MIP) film demonstrated efficient molecular recognition and significant electrochemical responsiveness, enabling VB<sub>12</sub> detection in PBS buffer with an LOD of 0.5 μM within a linear range of 0.5–10 μM (R<sup>2</sup> = 0.991). Surface characterization revealed remarkable property changes during sensor fabrication. A significant reduction in the hydrophilicity of the VB<sub>12</sub>-AP-MIP film after template removal (TR) was observed due to the increased contact angle from 33.1° to 55.82° (<em>p</em> < 0.05). Morphological analysis using atomic force microscopy (AFM) showed the highest roughness (90.57 nm) for the VB<sub>12</sub>-AP-MIP film, decreasing after TR (30.56 nm) and increasing upon VB<sub>12</sub> capture (46.04 nm). Scanning electron microscopy (SEM) revealed smoother, more uniform surfaces for MIP films than non-imprinted polymer (NIP) films, indicating the template's critical role in polymerization. The developed electro-MIP sensor exhibited good template-selectivity and a 15-day storage stability at 4 °C. The electrochemical VB<sub>12</sub>-AP-MIP sensor successfully detected VB<sub>12</sub> in digested microcapsules under simulated salivary (3.30 ± 0.46 μM), gastric (64.87 ± 2.66 μM), and intestinal (76.68 ± 2.16 μM) conditions. The VB<sub>12</sub> analysis using HPLC and UV–Vis spectrophotometry showed that the electro-MIP sensor outcomes were comparable to those obtained with HPLC. The developed electro-MIP sensor would be a promising tool for determining VB<sub>12</sub> levels in complex biological samples.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100759"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A label-free gold nanoparticles functionalized peptide dendrimer biosensor for visual detection of breakthrough infections in COVID-19 vaccinated patients

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2024.100718

Naveen Kumar , Ashutosh Singh , Preeti Dhaka , Ankur Singh , Pragya Agarwala , Kuldeep Sharma , Anudita Bhargava , Sandeep Bhatia , Thomas Launey , Rahul Kaushik , Shailly Tomar , Aniket Sanyal

Given the global implementation of effective COVID-19 vaccines, which do not confer complete immunity, it is crucial to monitor the occurrence of breakthrough infections, particularly against newly emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. Hence, we developed a label-free colorimetric assay using gold nanoparticles (GNPs) functionalized with a peptide dendrimer incorporating highly reactive epitopes of the nucleocapsid (N) protein. This assay relies on the tween-20 induced colorimetric changes caused by the aggregation of peptide dendrimer-coated GNPs in the absence of anti- SARS-CoV-2 N antibodies, and vice versa. Transmission electron microscopy, dynamic light scattering, and circular dichroism spectroscopy analyses all showed the formation of a uniform and highly stable coating of the peptide dendrimer over GNPs. Surface plasmon resonance experiments have demonstrated a strong binding affinity for the peptide dendrimer and anti- SARS-CoV-2 N antibodies, with a K_D value of 525 nM. To validate the proof-of-concept, we have tested this assay on seventy human serum samples, and receiver operating characteristic curve analysis demonstrated high diagnostic sensitivity (88.89 %) and specificity (100 %). This approach opens up new avenues for the development of simple and rapid diagnostic assays for identifying antibodies against viral infections and other pathogens.

{"title":"A label-free gold nanoparticles functionalized peptide dendrimer biosensor for visual detection of breakthrough infections in COVID-19 vaccinated patients","authors":"Naveen Kumar , Ashutosh Singh , Preeti Dhaka , Ankur Singh , Pragya Agarwala , Kuldeep Sharma , Anudita Bhargava , Sandeep Bhatia , Thomas Launey , Rahul Kaushik , Shailly Tomar , Aniket Sanyal","doi":"10.1016/j.sbsr.2024.100718","DOIUrl":"10.1016/j.sbsr.2024.100718","url":null,"abstract":"<div><div>Given the global implementation of effective COVID-19 vaccines, which do not confer complete immunity, it is crucial to monitor the occurrence of breakthrough infections, particularly against newly emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants. Hence, we developed a label-free colorimetric assay using gold nanoparticles (GNPs) functionalized with a peptide dendrimer incorporating highly reactive epitopes of the nucleocapsid (N) protein. This assay relies on the tween-20 induced colorimetric changes caused by the aggregation of peptide dendrimer-coated GNPs in the absence of anti- SARS-CoV-2 N antibodies, and <em>vice versa</em>. Transmission electron microscopy, dynamic light scattering, and circular dichroism spectroscopy analyses all showed the formation of a uniform and highly stable coating of the peptide dendrimer over GNPs. Surface plasmon resonance experiments have demonstrated a strong binding affinity for the peptide dendrimer and anti- SARS-CoV-2 N antibodies, with a <em>K</em><sub>D</sub> value of 525 nM. To validate the proof-of-concept, we have tested this assay on seventy human serum samples, and receiver operating characteristic curve analysis demonstrated high diagnostic sensitivity (88.89 %) and specificity (100 %). This approach opens up new avenues for the development of simple and rapid diagnostic assays for identifying antibodies against viral infections and other pathogens.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100718"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing sensitivity with laser-scribed graphene interdigitated electrodes in water quality monitoring

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2024.100731

Ceren Durmus , Abdullah Bukhamsin , José Ilton de Oliveira Filho , Khaled Nabil Salama

Conventional methods for monitoring water quality are often time-consuming, expensive, and lack sensitivity, making it difficult to detect contaminants before they enter the environment. Therefore, it is essential to develop sensing platforms that address these issues and that are capable of performing on-site detection. As such, in this study, we developed an electrochemical sensing platform for detecting pharmaceutical pollutants in water, particularly paracetamol (PCM) and acetylsalicylic acid (ASP). By minimizing the gap distance between the working and auxiliary electrodes of laser-scribed graphene interdigitated electrodes (LSG-IDEs), the sensitivity of the sensors was improved. The developed platform was compared to a standard LSGE design, and the LSG-IDEs achieved an 18.6-fold and 70-fold improvement in detection limits for PCM and ASP, respectively. The system was tested with real wastewater samples spiked with ASP and PCM, demonstrating its effectiveness in practical scenarios. Additionally, the system was successfully integrated with an on-site detection device, demonstrating its potential for real-time, portable water quality monitoring. The high sensitivity and low-cost of LSG-IDEs make them a suitable option for the monitoring of water quality and protecting public health.

{"title":"Advancing sensitivity with laser-scribed graphene interdigitated electrodes in water quality monitoring","authors":"Ceren Durmus , Abdullah Bukhamsin , José Ilton de Oliveira Filho , Khaled Nabil Salama","doi":"10.1016/j.sbsr.2024.100731","DOIUrl":"10.1016/j.sbsr.2024.100731","url":null,"abstract":"<div><div>Conventional methods for monitoring water quality are often time-consuming, expensive, and lack sensitivity, making it difficult to detect contaminants before they enter the environment. Therefore, it is essential to develop sensing platforms that address these issues and that are capable of performing on-site detection. As such, in this study, we developed an electrochemical sensing platform for detecting pharmaceutical pollutants in water, particularly paracetamol (PCM) and acetylsalicylic acid (ASP). By minimizing the gap distance between the working and auxiliary electrodes of laser-scribed graphene interdigitated electrodes (LSG-IDEs), the sensitivity of the sensors was improved. The developed platform was compared to a standard LSGE design, and the LSG-IDEs achieved an 18.6-fold and 70-fold improvement in detection limits for PCM and ASP, respectively. The system was tested with real wastewater samples spiked with ASP and PCM, demonstrating its effectiveness in practical scenarios. Additionally, the system was successfully integrated with an on-site detection device, demonstrating its potential for real-time, portable water quality monitoring. The high sensitivity and low-cost of LSG-IDEs make them a suitable option for the monitoring of water quality and protecting public health.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100731"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review on roles of electrochemical approaches in drug delivery

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100742

Ali R. Jalalvand, Mohammad Mehdi Karami

The advancement of drug delivery systems (DDS) has significantly enhanced the efficacy and safety of therapeutic agents addressing limitations such as poor bioavailability and non-specific distribution associated with traditional methods. This article provides a comprehensive review of the role of electrochemical approaches in DDS, highlighting their precision, minimally invasive nature and ability to provide targeted and localized therapy. Electrochemical methods including voltammetry, amperometry and potentiometry offer unparalleled control over drug release kinetics and distribution, presenting significant advantages for the treatment of chronic and complex diseases like cancer, cardiovascular disorders, and neurological conditions. The integration of biotechnology, materials science and nanotechnology has led to the development of sophisticated DDS that respond to specific biological signals. This review discusses the fundamental principles of electrochemistry, various electrochemical techniques, and their applications in DDS alongside current innovations and future research directions in the field. The potential of electrochemical approaches to revolutionize drug delivery by providing precise, controlled, and targeted therapeutic interventions is emphasized, underscoring their growing importance in personalized medicine and future biomedical applications.

{"title":"A comprehensive review on roles of electrochemical approaches in drug delivery","authors":"Ali R. Jalalvand, Mohammad Mehdi Karami","doi":"10.1016/j.sbsr.2025.100742","DOIUrl":"10.1016/j.sbsr.2025.100742","url":null,"abstract":"<div><div>The advancement of drug delivery systems (DDS) has significantly enhanced the efficacy and safety of therapeutic agents addressing limitations such as poor bioavailability and non-specific distribution associated with traditional methods. This article provides a comprehensive review of the role of electrochemical approaches in DDS, highlighting their precision, minimally invasive nature and ability to provide targeted and localized therapy. Electrochemical methods including voltammetry, amperometry and potentiometry offer unparalleled control over drug release kinetics and distribution, presenting significant advantages for the treatment of chronic and complex diseases like cancer, cardiovascular disorders, and neurological conditions. The integration of biotechnology, materials science and nanotechnology has led to the development of sophisticated DDS that respond to specific biological signals. This review discusses the fundamental principles of electrochemistry, various electrochemical techniques, and their applications in DDS alongside current innovations and future research directions in the field. The potential of electrochemical approaches to revolutionize drug delivery by providing precise, controlled, and targeted therapeutic interventions is emphasized, underscoring their growing importance in personalized medicine and future biomedical applications.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100742"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical aptasensor for ultrasensitive detection of glycated hemoglobin (HbA1c) using gold-modified SPCE

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100765

Serly Zuliska , Irkham , Salma Nur Zakiyyah , Yeni Wahyuni Hartati , Yasuaki Einaga , Iman Permana Maksum

Accurate monitoring of diabetes mellitus (DM) biomarkers, particularly glycated hemoglobin (HbA1c), is essential for assessing long-term blood glucose control, optimizing therapeutic strategies, and preventing diabetes-related complications. To enhance the efficiency of this monitoring, electrochemical aptasensors based on screen-printed carbon electrodes (SPCE) modified with gold nanoparticles (AuNP) have been developed. The results demonstrate that the electrodeposition technique produces a uniform AuNP distribution and significantly increases the current responses of K₃[Fe(CN)₆] compared to bare SPCE. Aptamer immobilization using a streptavidin-biotin system via linker-mediated attachment exhibits high affinity for HbA1c, as evidenced by a significant decrease in current upon target binding.The analytical performance of the aptasensor shows a low detection limit of 8.34 pg/mL, with a linear detection range of 1 to 10⁴ pg/mL. Moreover, the aptasensor exhibits good selectivity for HbA1c over hemoglobin, glucose, and palmitic acid, with a selectivity value of 82.56 %. Stability tests indicate that the sensor retains 90.38 % of its activity after 70 days of storage, confirming its reliability for HbA1c detection. This study contributes to the advancement of effective electrochemical biosensors for diabetes monitoring, enabling early diagnosis and improved disease management.

{"title":"Electrochemical aptasensor for ultrasensitive detection of glycated hemoglobin (HbA1c) using gold-modified SPCE","authors":"Serly Zuliska , Irkham , Salma Nur Zakiyyah , Yeni Wahyuni Hartati , Yasuaki Einaga , Iman Permana Maksum","doi":"10.1016/j.sbsr.2025.100765","DOIUrl":"10.1016/j.sbsr.2025.100765","url":null,"abstract":"<div><div>Accurate monitoring of diabetes mellitus (DM) biomarkers, particularly glycated hemoglobin (HbA1c), is essential for assessing long-term blood glucose control, optimizing therapeutic strategies, and preventing diabetes-related complications. To enhance the efficiency of this monitoring, electrochemical aptasensors based on screen-printed carbon electrodes (SPCE) modified with gold nanoparticles (AuNP) have been developed. The results demonstrate that the electrodeposition technique produces a uniform AuNP distribution and significantly increases the current responses of K₃[Fe(CN)₆] compared to bare SPCE. Aptamer immobilization using a streptavidin-biotin system via linker-mediated attachment exhibits high affinity for HbA1c, as evidenced by a significant decrease in current upon target binding.The analytical performance of the aptasensor shows a low detection limit of 8.34 pg/mL, with a linear detection range of 1 to 10<sup>4</sup> pg/mL. Moreover, the aptasensor exhibits good selectivity for HbA1c over hemoglobin, glucose, and palmitic acid, with a selectivity value of 82.56 %. Stability tests indicate that the sensor retains 90.38 % of its activity after 70 days of storage, confirming its reliability for HbA1c detection. This study contributes to the advancement of effective electrochemical biosensors for diabetes monitoring, enabling early diagnosis and improved disease management.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100765"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143421910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CRISPR/Cas12a electrochemical biosensing to detect pig mtDNA D-loop for ensuring food authenticity

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100755

Muhammad Ihda H.L. Zein , Clianta Yudin Kharismasari , Ari Hardianto , Salma Nur Zakiyyah , Riezki Amalia , Mehmet Ozsoz , Mara Mirasoli , Irkham , Yeni Wahyuni Hartati

Ensuring safe and accurately labeled food products is crucial for communities worldwide. One significant concern in food safety is the unintended inclusion of pork in products, which impacts food labeling, religious dietary practices, legal standards, and public health. Detecting such contamination is challenging due to complex food processing, requiring advanced methods for accurate identification. A CRISPR/Cas12a-based electrochemical biosensor has garnered attention for its rapidity, portability, high sensitivity, and specificity, leveraging trans-cleavage activity for precise detection. This study utilized a modified electrode, SPCE/Ceria/STV/Biotin-ssDNA-Methylene Blue (MB), characterized by SEM/EDX and voltammetry methods to examine morphology and electrochemical behavior. Optimization using the Box-Behnken Design (BBD) yielded ideal conditions: 30 min STV incubation, 1.5 μM probe concentration, 240 min probe incubation, 0.1 % BSA concentration, 30 min target incubation, and 2 μL target volume. These parameters enabled the development of an ultrasensitive biosensor with detection and quantification limits of 4 fM and 71 fM, respectively. Operating within a range of 10 nM to 100 fM, the biosensor achieved 1.1 % RSD. This CRISPR-based biosensor successfully detected pig mtDNA in raw and processed meat samples, achieving a 100.82 % recovery rate for corned samples and maintaining stability for 14 days. These findings highlight the biosensor's potential as a rapid, stable, ultrasensitive, and ultraspecific alternative for food monitoring. Its robustness makes it particularly suitable for ensuring the authenticity of animal-derived food products susceptible to counterfeiting.

{"title":"A CRISPR/Cas12a electrochemical biosensing to detect pig mtDNA D-loop for ensuring food authenticity","authors":"Muhammad Ihda H.L. Zein , Clianta Yudin Kharismasari , Ari Hardianto , Salma Nur Zakiyyah , Riezki Amalia , Mehmet Ozsoz , Mara Mirasoli , Irkham , Yeni Wahyuni Hartati","doi":"10.1016/j.sbsr.2025.100755","DOIUrl":"10.1016/j.sbsr.2025.100755","url":null,"abstract":"<div><div>Ensuring safe and accurately labeled food products is crucial for communities worldwide. One significant concern in food safety is the unintended inclusion of pork in products, which impacts food labeling, religious dietary practices, legal standards, and public health. Detecting such contamination is challenging due to complex food processing, requiring advanced methods for accurate identification. A CRISPR/Cas12a-based electrochemical biosensor has garnered attention for its rapidity, portability, high sensitivity, and specificity, leveraging trans-cleavage activity for precise detection. This study utilized a modified electrode, SPCE/Ceria/STV/Biotin-ssDNA-Methylene Blue (MB), characterized by SEM/EDX and voltammetry methods to examine morphology and electrochemical behavior. Optimization using the Box-Behnken Design (BBD) yielded ideal conditions: 30 min STV incubation, 1.5 μM probe concentration, 240 min probe incubation, 0.1 % BSA concentration, 30 min target incubation, and 2 μL target volume. These parameters enabled the development of an ultrasensitive biosensor with detection and quantification limits of 4 fM and 71 fM, respectively. Operating within a range of 10 nM to 100 fM, the biosensor achieved 1.1 % RSD. This CRISPR-based biosensor successfully detected pig mtDNA in raw and processed meat samples, achieving a 100.82 % recovery rate for corned samples and maintaining stability for 14 days. These findings highlight the biosensor's potential as a rapid, stable, ultrasensitive, and ultraspecific alternative for food monitoring. Its robustness makes it particularly suitable for ensuring the authenticity of animal-derived food products susceptible to counterfeiting.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100755"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesis of biocompatible fluorescent carbon dots from bitter gourd for effective metal sensing and biological applications

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100751

V. Renuga , X. Vinoliya , A. Dinesh , S. Suthakaran , Manikandan Ayyar , V. Mohanavel , Lalitha Gnanasekaran , Prabhu Paramasivam , R. Bousbih , M. Khalid Hossain , A. Rajendran , S. Santhoshkumar

Highly fluorescent Carbon dots (C-dots) were synthesized from the Bitter Gourd by simple hydrothermal method without the addition of any surfactant or additives. The optical properties, luminescence, phase formation and crystal structure were measured using UV–Visible spectroscopy (UV–Vis), Photoluminescence (PL), X-ray diffraction (XRD) and Transmission electron microscopy (TEM) by varying the medium from acidic to basic by changing its pH (2−12) and temperature range (180-300^οC) to assess the exact nature and properties of the synthesized C-dots. The synthesized C-dots have wide range of applications from metal sensing to bioimaging and optoelectronics. The antimicrobial properties of the prepared C-dots were also analyzed using Klebsiellapneumoniae and Staphylococcus aureus microbes and found that the prepared C-dots have better antimicrobial activity in both microbes with better efficiency due to its smaller size. The challenges and perspectives of the synthesized C-dots from Bitter Gourd were also discussed.

{"title":"Green synthesis of biocompatible fluorescent carbon dots from bitter gourd for effective metal sensing and biological applications","authors":"V. Renuga , X. Vinoliya , A. Dinesh , S. Suthakaran , Manikandan Ayyar , V. Mohanavel , Lalitha Gnanasekaran , Prabhu Paramasivam , R. Bousbih , M. Khalid Hossain , A. Rajendran , S. Santhoshkumar","doi":"10.1016/j.sbsr.2025.100751","DOIUrl":"10.1016/j.sbsr.2025.100751","url":null,"abstract":"<div><div>Highly fluorescent Carbon dots (C-dots) were synthesized from the Bitter Gourd by simple hydrothermal method without the addition of any surfactant or additives. The optical properties, luminescence, phase formation and crystal structure were measured using UV–Visible spectroscopy (UV–Vis), Photoluminescence (PL), X-ray diffraction (XRD) and Transmission electron microscopy (TEM) by varying the medium from acidic to basic by changing its pH (2−12) and temperature range (180-300<sup>ο</sup>C) to assess the exact nature and properties of the synthesized C-dots. The synthesized C-dots have wide range of applications from metal sensing to bioimaging and optoelectronics. The antimicrobial properties of the prepared C-dots were also analyzed using <em>Klebsiellapneumoniae</em> and <em>Staphylococcus aureus</em> microbes and found that the prepared C-dots have better antimicrobial activity in both microbes with better efficiency due to its smaller size. The challenges and perspectives of the synthesized C-dots from Bitter Gourd were also discussed.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100751"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Smart sensing of creatinine in urine samples: Leveraging Cu-nanowires/MoS2 quantum dots and machine learning

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2024.100727

Geethukrishnan , Paresh Prakash Bagde , Sammishra KH , Chandranath Adak , Rajendra P. Shukla , Kiran Kumar Tadi

Serum creatinine (CRT) levels are key biomarkers for diagnosing, staging, and monitoring renal disease in clinical practice. In this work, copper nanowires (CuNW), and Molybdenum disulfide quantum dots (MSQD) modified glassy carbon electrode (GCE) were chosen to demonstrate the electrochemical detection of CRT in complex mixture and urine samples. The materials were characterized using various physical characterizations such as FESEM, XRD, UV, PL, and FT-Raman. The electrocatalytic activity of the sensor was investigated using cyclic voltammetry (CV), and differential pulse voltammetry (DPVs). Despite the elevated sensitivity and cost-effectiveness of electrochemical sensors, the performance of the sensors is constrained by the existence of interfering species that generate conflicting and overlapping electrochemical signatures. In order to address this issue, we implemented a machine learning (ML) approach to accurately quantify CRT levels in complex mixtures, as well as in urine samples. The ML algorithms employed are trained and tested on a large dataset, allowing them to effectively capture and analyze the variance in the electrochemical signatures, demonstrating the application of artificial intelligence. The proposed sensor exhibits linearity from 1.96 μM to 966.0 μM and shows the best performance in terms of limit-of-detection (LOD) of 2.3 μM in a complex mixture and 0.001 μM in real urine samples, with RMSE of 0.2 and 0.017 μM using artificial neural network and random forest ML models respectively. We anticipate that by further miniaturization of these sensors into point-of-care testing devices, renal diseases can be managed effectively.

{"title":"Smart sensing of creatinine in urine samples: Leveraging Cu-nanowires/MoS2 quantum dots and machine learning","authors":"Geethukrishnan , Paresh Prakash Bagde , Sammishra KH , Chandranath Adak , Rajendra P. Shukla , Kiran Kumar Tadi","doi":"10.1016/j.sbsr.2024.100727","DOIUrl":"10.1016/j.sbsr.2024.100727","url":null,"abstract":"<div><div>Serum creatinine (CRT) levels are key biomarkers for diagnosing, staging, and monitoring renal disease in clinical practice. In this work, copper nanowires (CuNW), and Molybdenum disulfide quantum dots (MSQD) modified glassy carbon electrode (GCE) were chosen to demonstrate the electrochemical detection of CRT in complex mixture and urine samples. The materials were characterized using various physical characterizations such as FESEM, XRD, UV, PL, and FT-Raman. The electrocatalytic activity of the sensor was investigated using cyclic voltammetry (CV), and differential pulse voltammetry (DPVs). Despite the elevated sensitivity and cost-effectiveness of electrochemical sensors, the performance of the sensors is constrained by the existence of interfering species that generate conflicting and overlapping electrochemical signatures. In order to address this issue, we implemented a machine learning (ML) approach to accurately quantify CRT levels in complex mixtures, as well as in urine samples. The ML algorithms employed are trained and tested on a large dataset, allowing them to effectively capture and analyze the variance in the electrochemical signatures, demonstrating the application of artificial intelligence. The proposed sensor exhibits linearity from 1.96 μM to 966.0 μM and shows the best performance in terms of limit-of-detection (LOD) of 2.3 μM in a complex mixture and 0.001 μM in real urine samples, with RMSE of 0.2 and 0.017 μM using artificial neural network and random forest ML models respectively. We anticipate that by further miniaturization of these sensors into point-of-care testing devices, renal diseases can be managed effectively.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100727"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0