Sensing and Bio-Sensing Research最新文献_第3页

Adaptive photonic and microfluidic chip packaging via 3D alignment structures 基于三维排列结构的自适应光子和微流控芯片封装

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-12-18 DOI: 10.1016/j.sbsr.2025.100944

Rouven H. Klenk , Syeda Rameen , Christian Schweikert , Wolfgang Vogel , Markus Greul , Mathias Kaschel , Manfred Berroth , Georg Rademacher , Michael Heymann

Combining photonic integrated circuits (PICs) with microfluidic devices offers new possibilities for optofluidic lab-on-a-chip applications. Their accurate assembly and packaging, particularly with precise alignment, remain challenging. This work presents a versatile optofluidic integration of photonic and microfluidic chips, designed for seamless implementation in diverse laboratory environments. A 3D-printed holder for stable, cost-effective, and convenient fiber-to-chip bonding is used to configure optical in- and outputs. It can be readily adapted to accommodate various grating coupler designs. The microfluidic chip side is fabricated via soft-lithography in polydimethylsiloxane (PDMS), and positioned onto the photonic chip via cone-shaped alignment guides that were printed directly onto it. For this, the conventional PDMS plasma bonding process was adapted for passive self-alignment with the PIC chip between the 100 μm-wide microfluidic channel and two grating couplers, each measuring 15 μm in length and 50 μm in width. In a proof-of-principle experiment, we detect the fluorescence of a fluorescein solution inside the microfluidic channels by delivering excitation and emission light through dedicated grating couplers. This configuration can address multiple sampling points along an optofluidic system to multiplex assays in a way that would be challenging to realize with conventional optical fibers.

光子集成电路（PICs）与微流控器件的结合为光电流控芯片实验室的应用提供了新的可能性。它们的精确组装和包装，特别是精确对准，仍然具有挑战性。这项工作提出了光子和微流控芯片的多功能光流集成，设计用于在不同的实验室环境中无缝实现。3d打印支架稳定，经济高效，方便的光纤到芯片键合用于配置光学输入和输出。它可以很容易地适应各种光栅耦合器的设计。微流控芯片侧通过软光刻技术在聚二甲基硅氧烷（PDMS）中制造，并通过直接印刷在光子芯片上的锥形对齐导轨定位到光子芯片上。为此，采用传统的PDMS等离子体键合工艺，使PIC芯片在100 μm宽的微流控通道和两个长15 μm、宽50 μm的光栅耦合器之间被动自对准。在一个原理验证实验中，我们通过专用光栅耦合器提供激发和发射光来检测微流体通道内荧光素溶液的荧光。这种配置可以沿着光流体系统处理多个采样点，以一种传统光纤难以实现的方式进行多路分析。

{"title":"Adaptive photonic and microfluidic chip packaging via 3D alignment structures","authors":"Rouven H. Klenk , Syeda Rameen , Christian Schweikert , Wolfgang Vogel , Markus Greul , Mathias Kaschel , Manfred Berroth , Georg Rademacher , Michael Heymann","doi":"10.1016/j.sbsr.2025.100944","DOIUrl":"10.1016/j.sbsr.2025.100944","url":null,"abstract":"<div><div>Combining photonic integrated circuits (PICs) with microfluidic devices offers new possibilities for optofluidic lab-on-a-chip applications. Their accurate assembly and packaging, particularly with precise alignment, remain challenging. This work presents a versatile optofluidic integration of photonic and microfluidic chips, designed for seamless implementation in diverse laboratory environments. A 3D-printed holder for stable, cost-effective, and convenient fiber-to-chip bonding is used to configure optical in- and outputs. It can be readily adapted to accommodate various grating coupler designs. The microfluidic chip side is fabricated via soft-lithography in polydimethylsiloxane (PDMS), and positioned onto the photonic chip via cone-shaped alignment guides that were printed directly onto it. For this, the conventional PDMS plasma bonding process was adapted for passive self-alignment with the PIC chip between the 100 μm-wide microfluidic channel and two grating couplers, each measuring 15 μm in length and 50 μm in width. In a proof-of-principle experiment, we detect the fluorescence of a fluorescein solution inside the microfluidic channels by delivering excitation and emission light through dedicated grating couplers. This configuration can address multiple sampling points along an optofluidic system to multiplex assays in a way that would be challenging to realize with conventional optical fibers.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100944"},"PeriodicalIF":4.9,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic platform combined with a dark field imaging system for quantification of E. coli contamination in water 微流控平台结合暗场成像系统定量测定水中大肠杆菌污染

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-12-16 DOI: 10.1016/j.sbsr.2025.100945

Anna Malec , Christoph Haiden , Georgios Kokkinis , Ioanna Giouroudi

In this paper, we present a method for detecting and quantifying pathogens in water samples. The method proposes a portable dark field imaging and analysis system for quantifying E. coli concentrations in water after being labeled with magnetic particles. The system utilizes the tracking of moving micro/nano objects close to or below the optical resolution limit confined in small sample volumes (∼ 10 μl). In particular, the system analyzes the effect of volumetric changes due to bacteria conjugation to magnetic microparticles (MP) on their Brownian motion while being suspended in liquid buffer solution. The method allows for a simple inexpensive implementation and the possibility to be used as point-of-need testing system. Indeed, a working prototype is demonstrated with the capacity of quantifying E. coli colony forming units (CFU) at a range of 1 × 10³ - 6 × 10³ CFU/mL.

本文提出了一种检测和定量水样中病原菌的方法。该方法提出了一种便携式暗场成像分析系统，用于磁性颗粒标记后定量水中大肠杆菌浓度。该系统利用跟踪接近或低于光学分辨率限制的移动微/纳米物体，限制在小样品体积（~ 10 μl）中。特别是，该系统分析了细菌与磁性微粒（MP）偶联引起的体积变化对悬浮在液体缓冲溶液中的布朗运动的影响。该方法允许一个简单廉价的实现，并有可能被用作需求点测试系统。事实上，一个工作原型被证明具有在1 × 103 - 6 × 103 CFU/mL范围内定量大肠杆菌菌落形成单位（CFU）的能力。

引用次数: 0

Engineering an olfactory biosensor for preferential monitoring medium-chain fatty acids in wastewater 设计一种优先监测废水中中链脂肪酸的嗅觉生物传感器

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-12-11 DOI: 10.1016/j.sbsr.2025.100943

Yingjian Liu , Jiahui Sun , Fei Wang , Yu Zheng , Junhong He , Weihong Liu

Monitoring medium-chain fatty acids (MCFAs) in wastewater is essential for environmental protection and resource recovery, as these compounds contribute to malodor issues while holding significant value as precursors to biofuels and fine chemicals. However, conventional techniques such as GC–MS and HPLC are limited by operational complexity and high cost, restricting their applicability for real-time monitoring. To overcome these challenges, we developed a biomimetic biosensor based on engineered human olfactory receptor OR51L1, a member of the OR51 subfamily known for its affinity toward short- and medium-chain fatty acids. Through systematic functional screening, we found that the wild-type OR51L1 receptor responds primarily to pentanoic acid (C5) and hexanoic acid (C6). Using structure-guided mutagenesis, we generated the OR51L1(T161A) mutant, which exhibits enhanced sensitivity toward these MCFAs and, importantly, gains the ability to detect heptanoic acid (C7) — a ligand not recognized by the wild-type receptor. Molecular dynamics (MD) simulations indicated that the T161A substitution optimizes the ligand-binding pocket, promoting stronger interactions with MCFAs. The engineered receptor was incorporated into a cell-based sensing platform, enabling sensitive and selective detection of MCFAs at the micromolar level, and additionally demonstrated robustness through high reproducibility and strong anti-interference capability against common wastewater constituents. This work demonstrates the promise of protein-engineered olfactory receptors as versatile biosensing tools for monitoring environmentally relevant MCFAs, offering scalable strategies for wastewater management and resource recovery.

监测废水中的中链脂肪酸（MCFAs）对于环境保护和资源回收至关重要，因为这些化合物有助于产生恶臭问题，同时作为生物燃料和精细化学品的前体具有重要价值。然而，传统的气相色谱-质谱、高效液相色谱等技术由于操作复杂、成本高，限制了其实时监测的适用性。为了克服这些挑战，我们开发了一种基于工程人类嗅觉受体OR51L1的仿生生物传感器，OR51L1是OR51亚家族的成员，以其对短链和中链脂肪酸的亲和力而闻名。通过系统功能筛选，我们发现野生型OR51L1受体主要对戊酸（C5）和己酸（C6）有应答。利用结构导向诱变技术，我们产生了OR51L1（T161A）突变体，该突变体对这些mcfa的敏感性增强，重要的是，它获得了检测七烷酸（C7）的能力，这是一种野生型受体无法识别的配体。分子动力学（MD）模拟表明，T161A取代优化了配体结合袋，促进了与MCFAs更强的相互作用。该工程受体被整合到一个基于细胞的传感平台中，能够在微摩尔水平上对mcfa进行敏感和选择性检测，并且通过高重复性和对常见废水成分的强抗干扰能力显示出鲁棒性。这项工作证明了蛋白质工程嗅觉受体作为监测环境相关mcfa的多功能生物传感工具的前景，为废水管理和资源回收提供了可扩展的策略。

{"title":"Engineering an olfactory biosensor for preferential monitoring medium-chain fatty acids in wastewater","authors":"Yingjian Liu , Jiahui Sun , Fei Wang , Yu Zheng , Junhong He , Weihong Liu","doi":"10.1016/j.sbsr.2025.100943","DOIUrl":"10.1016/j.sbsr.2025.100943","url":null,"abstract":"<div><div>Monitoring medium-chain fatty acids (MCFAs) in wastewater is essential for environmental protection and resource recovery, as these compounds contribute to malodor issues while holding significant value as precursors to biofuels and fine chemicals. However, conventional techniques such as GC–MS and HPLC are limited by operational complexity and high cost, restricting their applicability for real-time monitoring. To overcome these challenges, we developed a biomimetic biosensor based on engineered human olfactory receptor OR51L1, a member of the OR51 subfamily known for its affinity toward short- and medium-chain fatty acids. Through systematic functional screening, we found that the wild-type OR51L1 receptor responds primarily to pentanoic acid (C5) and hexanoic acid (C6). Using structure-guided mutagenesis, we generated the OR51L1(T161A) mutant, which exhibits enhanced sensitivity toward these MCFAs and, importantly, gains the ability to detect heptanoic acid (C7) — a ligand not recognized by the wild-type receptor. Molecular dynamics (MD) simulations indicated that the T161A substitution optimizes the ligand-binding pocket, promoting stronger interactions with MCFAs. The engineered receptor was incorporated into a cell-based sensing platform, enabling sensitive and selective detection of MCFAs at the micromolar level, and additionally demonstrated robustness through high reproducibility and strong anti-interference capability against common wastewater constituents. This work demonstrates the promise of protein-engineered olfactory receptors as versatile biosensing tools for monitoring environmentally relevant MCFAs, offering scalable strategies for wastewater management and resource recovery.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100943"},"PeriodicalIF":4.9,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic Padlock Probe-based Rolling Circle Amplification for sensitive detection of mecA resistance gene in Staphylococcus aureus 基于微流控挂锁探针滚动圈扩增的金黄色葡萄球菌耐药基因检测

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-12-11 DOI: 10.1016/j.sbsr.2025.100941

Léo Baldenweck , Catarina Caneira , Jasmina Vidic

Antimicrobial resistance and the dissemination of resistance genes remain critical global health challenges, demanding efficient monitoring and detection tools. Methicillin-resistant Staphylococcus aureus (MRSA) is a prominent example, posing serious clinical and epidemiological concerns. Here, we developed a sensitive detection platform combining isothermal Padlock Probe-based Rolling Circle Amplification with a bead-based microfluidic biosensor and fluorescent readout for the detection of the mecA gene in S. aureus. The assay design was first validated using a complementary synthetic ssDNA target, then optimized with genomic dsDNA extracted from a laboratory strain. The optimized system was subsequently applied to clinical isolates from sepsis cases and complex matrices simulating natural habitats, including milk, serum, and salad. The biosensor achieved a detection limit of 10⁴ cells with 100 % specificity compared to PCR at an analysis time of two hour. All reaction steps were carried out sequentially within the microfluidic device, highlighting the strong potential of the device for full automation and for extension to additional bacterial genes.

抗微生物药物耐药性和耐药性基因的传播仍然是严重的全球卫生挑战，需要有效的监测和检测工具。耐甲氧西林金黄色葡萄球菌（MRSA）是一个突出的例子，引起了严重的临床和流行病学关注。在此，我们开发了一种灵敏的检测平台，将等温挂锁探针滚动圈扩增与基于微流控生物传感器和荧光读数相结合，用于检测金黄色葡萄球菌的mecA基因。实验设计首先使用互补的合成ssDNA靶标进行验证，然后使用从实验室菌株中提取的基因组dsDNA进行优化。优化后的系统随后应用于脓毒症病例的临床分离株和模拟自然环境的复杂基质，包括牛奶、血清和沙拉。与PCR相比，该生物传感器在两小时的分析时间内实现了104个细胞的检测限，特异性为100% %。所有的反应步骤都在微流控装置内依次进行，突出了该装置完全自动化和扩展到其他细菌基因的强大潜力。

{"title":"Microfluidic Padlock Probe-based Rolling Circle Amplification for sensitive detection of mecA resistance gene in Staphylococcus aureus","authors":"Léo Baldenweck , Catarina Caneira , Jasmina Vidic","doi":"10.1016/j.sbsr.2025.100941","DOIUrl":"10.1016/j.sbsr.2025.100941","url":null,"abstract":"<div><div>Antimicrobial resistance and the dissemination of resistance genes remain critical global health challenges, demanding efficient monitoring and detection tools. Methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) is a prominent example, posing serious clinical and epidemiological concerns. Here, we developed a sensitive detection platform combining isothermal Padlock Probe-based Rolling Circle Amplification with a bead-based microfluidic biosensor and fluorescent readout for the detection of the <em>mecA</em> gene in <em>S. aureus</em>. The assay design was first validated using a complementary synthetic ssDNA target, then optimized with genomic dsDNA extracted from a laboratory strain. The optimized system was subsequently applied to clinical isolates from sepsis cases and complex matrices simulating natural habitats, including milk, serum, and salad. The biosensor achieved a detection limit of 10<sup>4</sup> cells with 100 % specificity compared to PCR at an analysis time of two hour. All reaction steps were carried out sequentially within the microfluidic device, highlighting the strong potential of the device for full automation and for extension to additional bacterial genes.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100941"},"PeriodicalIF":4.9,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step Pd-NPs/Tyrosinase/Nafion biosensor for dopamine detection in the low μM range: ORR interference, defect-rich surface chemistry effects, and sensing pathway differentiation 用于低μM范围多巴胺检测的pd - np /酪氨酸酶/Nafion一步生物传感器：ORR干扰、富缺陷表面化学效应和传感途径分化

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-12-11 DOI: 10.1016/j.sbsr.2025.100942

M. Koch , H. Caparrotti , C. Pauly , O. Janka , M. Erzina , Y.E. Silina

A one-step (OS) biosensor for low micromolar (μM) dopamine (DA) detection was developed via co-electrodeposition from multiple palladium-based electrolyte containing tyrosinase (Tyr) and Nafion (Naf). The resulting OS biosensor modified with hybrid Pd-NPs/Tyr/Naf layer enabled DA detection in synthetic urine below 10 μM, with recovery rates of ∼98–112 %.

A key challenge was signal interference caused by indirect redox reactions between Pd-oxides (present on the surface of the electrodeposited Pd-NPs) and free DA, as well as from the oxygen reduction reaction (ORR). This issue was addressed by promoting the formation of non-stoichiometric PdOₓ (x < 1) through mild heating of the electrolyte during electrodeposition. This defect-rich surface suppressed ORR and non-enzymatic, ORR-mediated DA interactions, while retaining the catalytic activity of Pd-NPs toward dopamine-quinone as an enzymatic product.

Beyond its practical value, this work provides a framework for the controlled synthesis of OS-designed biosensing layers for enzymatic neurotransmitter detection at low μM levels, while minimizing interference from the intrinsic catalytic activity of electrodeposited Pd-NPs.

采用含有酪氨酸酶（Tyr）和萘酸（Naf）的多种钯基电解质共电沉积的方法，研制了一种用于低微摩尔（μM）多巴胺（DA）检测的一步式（OS）生物传感器。用混合Pd-NPs/Tyr/Naf层修饰的OS生物传感器可以在10 μM以下的合成尿液中检测DA，回收率为98 - 112%。一个关键的挑战是pd -氧化物（存在于电沉积Pd-NPs表面）与游离DA之间的间接氧化还原反应以及氧还原反应（ORR）引起的信号干扰。这个问题是通过在电沉积过程中通过轻微加热电解质来促进非化学计量PdOₓ（x < 1）的形成来解决的。这种富含缺陷的表面抑制ORR和非酶、ORR介导的DA相互作用，同时保留Pd-NPs对酶促产物多巴胺-醌的催化活性。除了它的实用价值之外，这项工作为在低μM水平下控制合成os设计的生物传感层提供了一个框架，用于酶促神经递质检测，同时最大限度地减少了电沉积pd - np的内在催化活性的干扰。

{"title":"One-step Pd-NPs/Tyrosinase/Nafion biosensor for dopamine detection in the low μM range: ORR interference, defect-rich surface chemistry effects, and sensing pathway differentiation","authors":"M. Koch , H. Caparrotti , C. Pauly , O. Janka , M. Erzina , Y.E. Silina","doi":"10.1016/j.sbsr.2025.100942","DOIUrl":"10.1016/j.sbsr.2025.100942","url":null,"abstract":"<div><div>A one-step (OS) biosensor for low micromolar (μM) dopamine (DA) detection was developed <em>via</em> co-electrodeposition from multiple palladium-based electrolyte containing tyrosinase (Tyr) and Nafion (Naf). The resulting OS biosensor modified with hybrid Pd-NPs/Tyr/Naf layer enabled DA detection in synthetic urine below 10 μM, with recovery rates of ∼98–112 %.</div><div>A key challenge was signal interference caused by indirect redox reactions between Pd-oxides (present on the surface of the electrodeposited Pd-NPs) and free DA, as well as from the oxygen reduction reaction (ORR). This issue was addressed by promoting the formation of non-stoichiometric PdOₓ (x < 1) through mild heating of the electrolyte during electrodeposition. This defect-rich surface suppressed ORR and non-enzymatic, ORR-mediated DA interactions, while retaining the catalytic activity of Pd-NPs toward dopamine-quinone as an enzymatic product.</div><div>Beyond its practical value, this work provides a framework for the controlled synthesis of OS-designed biosensing layers for enzymatic neurotransmitter detection at low μM levels, while minimizing interference from the intrinsic catalytic activity of electrodeposited Pd-NPs.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100942"},"PeriodicalIF":4.9,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric and time-resolved fluorescence lateral flow immunoassays for rapid screening of thrombosis and accurate quantification of D-dimer in human plasma 比色法和时间分辨荧光侧流免疫分析法用于快速筛选血栓形成和准确定量人血浆中d -二聚体

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-12-10 DOI: 10.1016/j.sbsr.2025.100940

Zhenyu Zhou , Xin'er Hu , Xinyi Xia , Meixiang Yu , Ningqi Xia , Jiayu Gu , Yan Cao , Hai Zhang

D-dimer is a soluble fibrin degradation product, and usually serves as a biomarker for screening and diagnosis of venous thromboembolism (VTE). Immunoturbidimetry is the most commonly used detection method in clinical practice, but it cannot achieve point-of-care testing (POCT) and on-site detection. In this study, two lateral flow immunochromatographic assay (LFIA), colloidal gold lateral flow immunoassay (CG-LFIA) and time-resolved fluorescence microsphere lateral flow immunoassay (TRFM-LFIA), were established for rapid qualification and accurate quantification of D-dimer in human plasma. Both analytical methods were rapid, simple, and capable of on-site monitoring without complex sample pretreatment. The CG-LFIA achieved rapid qualitative assessment based on the visible color intensity of the strips and on-site quantitative detection through strip image scanning. Compared with CG-LFIA, the TRFM-LFIA can provide a wider dynamic range and superior sensitivity for D-dimer detection, although it does not allow for rapid qualification. After systematic optimization and rigorous methodological validation, the detection limit of CG-LFIA was 40 ng/mL with a dynamic range of 40–1280 ng/mL, while TRFM-LFIA had a lower detection limit of 6.1 ng/mL and a wider dynamic range of 10–2000 ng/mL. Both methods demonstrated excellent consistency in detecting D-dimer in human plasma in comparison with immunoturbidimetry. Therefore, CG-LFIA is more suitable for rapid on-site screening of clinical thrombosis patients preliminarily, while TRFM-LFIA is more appropriate for rapid and accurate POCT quantification of D-dimer in human plasma. Both methods will provide more convenient testing services for clinical thrombus screening and thrombus progression assessment.

d -二聚体是一种可溶性纤维蛋白降解产物，通常作为筛选和诊断静脉血栓栓塞（VTE）的生物标志物。免疫比浊法是临床最常用的检测方法，但不能实现POCT和现场检测。本研究建立了胶体金侧流免疫分析法（CG-LFIA）和时间分辨荧光微球侧流免疫分析法（TRFM-LFIA）两种侧流免疫色谱法（LFIA），用于快速鉴定和准确定量人血浆中d -二聚体。两种分析方法快速、简便，无需复杂的样品前处理即可进行现场监测。CG-LFIA基于条带的可见色彩强度实现了快速定性评估，并通过条带图像扫描实现了现场定量检测。与CG-LFIA相比，TRFM-LFIA可以为d -二聚体检测提供更宽的动态范围和更高的灵敏度，尽管它不允许快速鉴定。经过系统优化和严格的方法学验证，CG-LFIA的检出限为40 ng/mL，动态范围为40 ~ 1280 ng/mL； TRFM-LFIA的检出限较低，为6.1 ng/mL，动态范围较宽，为10 ~ 2000 ng/mL。与免疫比浊法相比，两种方法在检测人血浆中d -二聚体方面表现出良好的一致性。因此，CG-LFIA更适合于临床血栓患者的初步快速现场筛查，而TRFM-LFIA更适合于人体血浆中d -二聚体POCT的快速准确定量。这两种方法都将为临床血栓筛查和血栓进展评估提供更便捷的检测服务。

{"title":"Colorimetric and time-resolved fluorescence lateral flow immunoassays for rapid screening of thrombosis and accurate quantification of D-dimer in human plasma","authors":"Zhenyu Zhou , Xin'er Hu , Xinyi Xia , Meixiang Yu , Ningqi Xia , Jiayu Gu , Yan Cao , Hai Zhang","doi":"10.1016/j.sbsr.2025.100940","DOIUrl":"10.1016/j.sbsr.2025.100940","url":null,"abstract":"<div><div>D-dimer is a soluble fibrin degradation product, and usually serves as a biomarker for screening and diagnosis of venous thromboembolism (VTE). Immunoturbidimetry is the most commonly used detection method in clinical practice, but it cannot achieve point-of-care testing (POCT) and on-site detection. In this study, two lateral flow immunochromatographic assay (LFIA), colloidal gold lateral flow immunoassay (CG-LFIA) and time-resolved fluorescence microsphere lateral flow immunoassay (TRFM-LFIA), were established for rapid qualification and accurate quantification of D-dimer in human plasma. Both analytical methods were rapid, simple, and capable of on-site monitoring without complex sample pretreatment. The CG-LFIA achieved rapid qualitative assessment based on the visible color intensity of the strips and on-site quantitative detection through strip image scanning. Compared with CG-LFIA, the TRFM-LFIA can provide a wider dynamic range and superior sensitivity for D-dimer detection, although it does not allow for rapid qualification. After systematic optimization and rigorous methodological validation, the detection limit of CG-LFIA was 40 ng/mL with a dynamic range of 40–1280 ng/mL, while TRFM-LFIA had a lower detection limit of 6.1 ng/mL and a wider dynamic range of 10–2000 ng/mL. Both methods demonstrated excellent consistency in detecting D-dimer in human plasma in comparison with immunoturbidimetry. Therefore, CG-LFIA is more suitable for rapid on-site screening of clinical thrombosis patients preliminarily, while TRFM-LFIA is more appropriate for rapid and accurate POCT quantification of D-dimer in human plasma. Both methods will provide more convenient testing services for clinical thrombus screening and thrombus progression assessment.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100940"},"PeriodicalIF":4.9,"publicationDate":"2025-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical determination of Pb(II) in paint factory effluent and hot spring water samples using chitosan-based Schiff base modified carbon paste electrode 壳聚糖基希夫碱改性碳糊电极电化学测定油漆厂废水和温泉水样品中的铅

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-12-08 DOI: 10.1016/j.sbsr.2025.100928

Kidus Ayalew Alemu , Genet Nurga Ansa , Tolera Marga Goshu , Mamo Gebrezgiabher , Siraye Esubalew Debebe , Tesfu Hailu Halefom

Pb(II) is a hazardous and non-biodegradable metal widely used in paint manufacturing and mining industries, posing health risks such as neurological disorders, kidney dysfunction, and increased blood pressure. Therefore, developing an easy, selective, and sensitive sensor for its detection is crucial. In this study, a chitosan-vanillin Schiff base (CVSB) was synthesized, and its successful formation was confirmed using spectroscopic and electrochemical techniques. Both bare and 10 % CVSB-modified carbon paste electrodes (CVSB-CPE) were employed to detect Pb(II), but the CVSB-CPE demonstrate significant electrocatalytic activity. Using an square wave anodic stripping voltammetry (SWASV) the sensor exhibited a linear response to Pb(II) ranging from 0.001 to 100 μM, with a limit of detection (LOD) of 0.57 nM. Additionally, repeatability tests showed a relative standard deviation (RSD) of 2.6 % over 17 consecutive measurements, while reproducibility across four independently prepared electrodes yielded a RSD of 1.05 %. The sensor also displayed high selectivity in the presence of potential interfering species, including Hg²⁺, K⁺, Cd²⁺, Na⁺, Ni²⁺, Fe³⁺, and Cu²⁺. The recovery tests conducted on factory effluent and hot spring water samples demonstrated a recovery ranging from 97.15 % to 113.49 % and 93.05 % to 108.01 %, respectively.

铅（II）是一种危险的、不可生物降解的金属，广泛用于油漆制造和采矿业，对健康构成风险，如神经系统疾病、肾功能障碍和血压升高。因此，开发一种简单、选择性强、灵敏度高的检测传感器至关重要。本研究合成了壳聚糖-香兰素席夫碱（CVSB），并通过波谱和电化学技术对其成功生成进行了验证。采用裸电极和10% cvsb修饰的碳糊电极（CVSB-CPE）检测Pb(II)，但CVSB-CPE表现出显著的电催化活性。采用方波阳极溶出伏安法（SWASV），传感器对Pb（II）的线性响应范围为0.001 ~ 100 μM，检出限为0.57 nM。此外，重复性测试显示，17次连续测量的相对标准偏差（RSD）为2.6%，而四个独立制备的电极的重复性RSD为1.05%。该传感器在Hg2+、K+、Cd2+、Na+、Ni2+、Fe3+和Cu2+等潜在干扰物质存在下也表现出较高的选择性。对工厂废水和温泉水样品进行了回收试验，回收率分别为97.15% ~ 113.49%和93.05% ~ 108.01%。

{"title":"Electrochemical determination of Pb(II) in paint factory effluent and hot spring water samples using chitosan-based Schiff base modified carbon paste electrode","authors":"Kidus Ayalew Alemu , Genet Nurga Ansa , Tolera Marga Goshu , Mamo Gebrezgiabher , Siraye Esubalew Debebe , Tesfu Hailu Halefom","doi":"10.1016/j.sbsr.2025.100928","DOIUrl":"10.1016/j.sbsr.2025.100928","url":null,"abstract":"<div><div>Pb(II) is a hazardous and non-biodegradable metal widely used in paint manufacturing and mining industries, posing health risks such as neurological disorders, kidney dysfunction, and increased blood pressure. Therefore, developing an easy, selective, and sensitive sensor for its detection is crucial. In this study, a chitosan-vanillin Schiff base (CVSB) was synthesized, and its successful formation was confirmed using spectroscopic and electrochemical techniques. Both bare and 10 % CVSB-modified carbon paste electrodes (CVSB-CPE) were employed to detect Pb(II), but the CVSB-CPE demonstrate significant electrocatalytic activity. Using an square wave anodic stripping voltammetry (SWASV) the sensor exhibited a linear response to Pb(II) ranging from 0.001 to 100 μM, with a limit of detection (LOD) of 0.57 nM. Additionally, repeatability tests showed a relative standard deviation (RSD) of 2.6 % over 17 consecutive measurements, while reproducibility across four independently prepared electrodes yielded a RSD of 1.05 %. The sensor also displayed high selectivity in the presence of potential interfering species, including Hg<sup>2+</sup>, K<sup>+</sup>, Cd<sup>2+</sup>, Na<sup>+</sup>, Ni<sup>2+</sup>, Fe<sup>3+</sup>, and Cu<sup>2+</sup>. The recovery tests conducted on factory effluent and hot spring water samples demonstrated a recovery ranging from 97.15 % to 113.49 % and 93.05 % to 108.01 %, respectively.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100928"},"PeriodicalIF":4.9,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic effect of silver, reduced graphene oxide and metal-organic framework-5 nanocomposite for the simultaneous voltammetric sensing of darunavir and naproxen pharmaceutical analytes using chemometric methods 银、还原氧化石墨烯和金属-有机框架-5纳米复合材料在达那韦和萘普生药物分析物同时伏安检测中的协同效应

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-12-06 DOI: 10.1016/j.sbsr.2025.100933

Maryam Lak, Seyed Karim Hassaninejad-Darzi

Darunavir (DRV), a protease inhibitor widely prescribed for antiretroviral therapy, and naproxen (NAP), a nonsteroidal anti-inflammatory drug, are frequently co-administered in patients with HIV-related inflammatory conditions, necessitating their accurate and simultaneous quantification in biological matrices. This study addresses the critical need for a sensitive and reliable analytical platform for the concurrent detection of DRV and NAP in complex biological samples. Conventional methods often suffer from poor selectivity due to overlapping oxidation peaks and limited sensitivity. To overcome these challenges, we developed a novel electrochemical nanosensor based on a silver-decorated reduced graphene oxide/metal–organic framework-5 nanocomposite–modified carbon paste electrode (Ag@RGO@MOF-5/CPE). The hybrid nanomaterial synergistically combines the high electrical conductivity of RGO, the large surface area of MOF-5, and the strong electrocatalytic activity of silver nanoparticles. Under optimized conditions via response surface methodology, the Ag@RGO@MOF-5/CPE exhibited enhanced current response and reduced oxidation overpotential for DRV, and NAP. However, due to overlapping oxidation potentials of drugs, partial least squares-1 (PLS-1) regression was implemented to enable accurate simultaneous determination, yielding detection limits of 0.20 μM for DRV and 0.40 μM for NAP in Britton-Robinson buffer (pH 7.0). The overlapping peaks were efficiently resolved via PLS-1 regression, and this approach was applied for detection of DRV, and NAP in human plasma and urine samples with satisfactory recovery. Overall, the integration of PLS-1 effectively eliminates signal overlap and offering a rapid, cost-effective, and robust alternative to chromatographic techniques for therapeutic drug monitoring in HIV/inflammation co-treatments, ultimately enhancing clinical safety and pharmacological efficiency.

Darunavir (DRV)，一种广泛用于抗逆转录病毒治疗的蛋白酶抑制剂，和萘普生（NAP），一种非甾体抗炎药，经常在hiv相关炎症患者中联合使用，需要在生物基质中准确和同时定量。该研究解决了对复杂生物样品中DRV和NAP同时检测的敏感可靠的分析平台的迫切需求。传统方法由于氧化峰重叠和灵敏度有限，往往存在选择性差的问题。为了克服这些挑战，我们开发了一种基于银装饰还原氧化石墨烯/金属有机框架-5纳米复合材料修饰碳糊电极（Ag@RGO@MOF-5/CPE）的新型电化学纳米传感器。该杂化纳米材料协同结合了还原氧化石墨烯的高导电性、MOF-5的大表面积和银纳米粒子的强电催化活性。在响应面法优化条件下，Ag@RGO@MOF-5/CPE对DRV和NAP的电流响应增强，氧化过电位降低。然而，由于药物的氧化电位重叠，采用偏最小二乘-1 （PLS-1）回归实现准确的同时测定，在Britton-Robinson缓冲液（pH 7.0）中，DRV的检出限为0.20 μM， NAP的检出限为0.40 μM。PLS-1回归有效地分离了重叠峰，并将该方法应用于人血浆和尿液中DRV和NAP的检测，回收率令人满意。总体而言，PLS-1的集成有效地消除了信号重叠，为HIV/炎症联合治疗的治疗药物监测提供了一种快速、经济、强大的色谱技术替代方案，最终提高了临床安全性和药理学效率。

{"title":"Synergistic effect of silver, reduced graphene oxide and metal-organic framework-5 nanocomposite for the simultaneous voltammetric sensing of darunavir and naproxen pharmaceutical analytes using chemometric methods","authors":"Maryam Lak, Seyed Karim Hassaninejad-Darzi","doi":"10.1016/j.sbsr.2025.100933","DOIUrl":"10.1016/j.sbsr.2025.100933","url":null,"abstract":"<div><div>Darunavir (DRV), a protease inhibitor widely prescribed for antiretroviral therapy, and naproxen (NAP), a nonsteroidal anti-inflammatory drug, are frequently co-administered in patients with HIV-related inflammatory conditions, necessitating their accurate and simultaneous quantification in biological matrices. This study addresses the critical need for a sensitive and reliable analytical platform for the concurrent detection of DRV and NAP in complex biological samples. Conventional methods often suffer from poor selectivity due to overlapping oxidation peaks and limited sensitivity. To overcome these challenges, we developed a novel electrochemical nanosensor based on a silver-decorated reduced graphene oxide/metal–organic framework-5 nanocomposite–modified carbon paste electrode (Ag@RGO@MOF-5/CPE). The hybrid nanomaterial synergistically combines the high electrical conductivity of RGO, the large surface area of MOF-5, and the strong electrocatalytic activity of silver nanoparticles. Under optimized conditions via response surface methodology, the Ag@RGO@MOF-5/CPE exhibited enhanced current response and reduced oxidation overpotential for DRV, and NAP. However, due to overlapping oxidation potentials of drugs, partial least squares-1 (PLS-1) regression was implemented to enable accurate simultaneous determination, yielding detection limits of 0.20 μM for DRV and 0.40 μM for NAP in Britton-Robinson buffer (pH 7.0). The overlapping peaks were efficiently resolved via PLS-1 regression, and this approach was applied for detection of DRV, and NAP in human plasma and urine samples with satisfactory recovery. Overall, the integration of PLS-1 effectively eliminates signal overlap and offering a rapid, cost-effective, and robust alternative to chromatographic techniques for therapeutic drug monitoring in HIV/inflammation co-treatments, ultimately enhancing clinical safety and pharmacological efficiency.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100933"},"PeriodicalIF":4.9,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Numerical simulation plasmonic biosensor based on metal-insulator-metal waveguide for label-free and non-invasive detection of prostate-specific antigen 基于金属-绝缘体-金属波导的无标签无创前列腺特异性抗原数值模拟等离子体生物传感器

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-12-06 DOI: 10.1016/j.sbsr.2025.100937

Asghar Molaei-Yeznabad , Hamid Bahador , Ghazal Abdi , Azadeh Nilghaz

In this work, a high-performance metal–insulator–metal plasmonic biosensor is presented and numerically investigated for label-free detection of prostate-specific antigen, which is an important biomarker for early-stage prostate cancer diagnosis. The device architecture utilizes a coupled semi-rectangular resonator side-coupled with the waveguide, in order to achieve a strong waveguide–cavity interaction. This structural configuration enables enhanced confinement of surface plasmon polaritons, and thereby improves light–matter interaction. The finite element method solution in commercial software COMSOL Multiphysics was used to do numerical simulations, where the structural parameters were optimized to maximize sensitivity, spectral sharpness, and achieve a narrower transmittance spectrum. After optimization, the designed sensor was systematically examined for its performance in prostate-specific antigen detection. The proposed sensor achieves a sensitivity of 1872.3 nm/RIU, a narrow full width at half maximum of 24 nm, a figure of merit of 76.6 RIU⁻¹, a Q-factor of 60.3, and a limit of detection of 0.0108 RIU. Furthermore, the simple geometry and compatibility with scalable nanofabrication techniques emphasize its potential application in real-time, portable, and point-of-care cancer diagnostics.

在这项工作中，提出了一种高性能金属-绝缘体-金属等离子体生物传感器，并对其进行了数值研究，用于无标记检测前列腺特异性抗原，这是早期前列腺癌诊断的重要生物标志物。该器件结构利用耦合半矩形谐振腔与波导侧耦合，以实现强波导-腔相互作用。这种结构配置可以增强表面等离子激元的极化约束，从而改善光-物质相互作用。利用商业软件COMSOL Multiphysics中的有限元解进行数值模拟，优化结构参数，最大限度地提高灵敏度和光谱清晰度，并实现更窄的透射光谱。优化后，对设计的传感器在前列腺特异性抗原检测中的性能进行了系统检验。该传感器的灵敏度为1872.3 nm/RIU，半宽窄为24 nm，品质系数为76.6 RIU−1，q因子为60.3，检测限为0.0108 RIU。此外，简单的几何结构和与可扩展纳米制造技术的兼容性强调了其在实时、便携式和即时癌症诊断方面的潜在应用。

{"title":"Numerical simulation plasmonic biosensor based on metal-insulator-metal waveguide for label-free and non-invasive detection of prostate-specific antigen","authors":"Asghar Molaei-Yeznabad , Hamid Bahador , Ghazal Abdi , Azadeh Nilghaz","doi":"10.1016/j.sbsr.2025.100937","DOIUrl":"10.1016/j.sbsr.2025.100937","url":null,"abstract":"<div><div>In this work, a high-performance metal–insulator–metal plasmonic biosensor is presented and numerically investigated for label-free detection of prostate-specific antigen, which is an important biomarker for early-stage prostate cancer diagnosis. The device architecture utilizes a coupled semi-rectangular resonator side-coupled with the waveguide, in order to achieve a strong waveguide–cavity interaction. This structural configuration enables enhanced confinement of surface plasmon polaritons, and thereby improves light–matter interaction. The finite element method solution in commercial software COMSOL Multiphysics was used to do numerical simulations, where the structural parameters were optimized to maximize sensitivity, spectral sharpness, and achieve a narrower transmittance spectrum. After optimization, the designed sensor was systematically examined for its performance in prostate-specific antigen detection. The proposed sensor achieves a sensitivity of 1872.3 nm/RIU, a narrow full width at half maximum of 24 nm, a figure of merit of 76.6 RIU<sup>−1</sup>, a Q-factor of 60.3, and a limit of detection of 0.0108 RIU. Furthermore, the simple geometry and compatibility with scalable nanofabrication techniques emphasize its potential application in real-time, portable, and point-of-care cancer diagnostics.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100937"},"PeriodicalIF":4.9,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a poly (alizarin red S)/EDTA/GCE sensor for rapid and reliable monitoring of methyl parathion pesticide 聚茜素红S /EDTA/GCE传感器快速可靠监测农药中甲基对硫磷的研制

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-12-05 DOI: 10.1016/j.sbsr.2025.100938

Andualem Ejigu , Molla Tefera , Adisie Kassa , Atnafu Guadie

Methyl parathion (MP) is a powerful organophosphate insecticide commonly used in agriculture, but it poses serious risks to both human health and environmental safety. This study presents a highly sensitive electrochemical sensor development based on a glassy carbon electrode (GCE) that has been modified in sequence with Ethylenediaminetetraacetic acid (EDTA) and electropolymerized with poly(Alizarin Red S) (poly(ARS)) for detecting MP. The poly(ARS)/EDTA/GCE was characterized with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The EDTA improves the surface functionality and adhesion, while the redox-active poly(ARS) film aids in electron transfer and offers selective sites for MP interaction. The sensor showed significantly better stability, catalytic performance, and electroactive surface area compared to the unmodified electrode.

Under optimized pH of 7.0, the sensor reached a limit of detection (LOD) of 0.5527 μM, a limit of quantification (LOQ) of 1.8425 μM, and a linear range from 0.05 to 140 μM, with a sensitivity of 0.1265 μA/μM. It exhibited excellent analytical capabilities, such as repeatability with a relative standard deviation (RSD) of 2.01 %, reproducibility across five independently crafted electrodes with an RSD of 3.13 %, and operational stability over five days with an RSD of 1.76 %. The practical utility of the sensor was validated through MP recovery studies on fresh vegetable samples, which achieved recoveries ranging from 98 % to 113 %.

In summary, the developed poly(ARS)/EDTA/GCE sensor provides an effective and reliable method for monitoring MP in both environmental and food safety contexts.

甲基对硫磷（Methyl parathion， MP）是一种农业常用的强效有机磷杀虫剂，但对人体健康和环境安全造成严重危害。本研究提出了一种基于玻璃碳电极（GCE）的高灵敏度电化学传感器，该电极经乙二胺四乙酸（EDTA）顺序修饰，并与聚茜素红S（聚（ARS））电聚合，用于检测MP。用循环伏安法（CV）和电化学阻抗谱（EIS）对聚(ARS)/EDTA/GCE进行了表征。EDTA改善了表面功能和粘附性，而氧化还原活性聚（ARS）膜有助于电子转移，并为MP相互作用提供了选择性位点。与未修饰的电极相比，该传感器表现出更好的稳定性、催化性能和电活性表面积。优化后的pH为7.0时，传感器的检出限为0.5527 μM，定量限为1.8425 μM，线性范围为0.05 ~ 140 μM，灵敏度为0.1265 μA/μM。它具有出色的分析能力，如重复性，相对标准偏差（RSD）为2.01%，重复性在5个独立制作的电极上，RSD为3.13%，5天内的操作稳定性，RSD为1.76%。通过对新鲜蔬菜样品的MP回收率研究，验证了该传感器的实用性，其回收率为98% ~ 113%。综上所述，所开发的聚(ARS)/EDTA/GCE传感器为环境和食品安全背景下的MP监测提供了一种有效可靠的方法。

{"title":"Development of a poly (alizarin red S)/EDTA/GCE sensor for rapid and reliable monitoring of methyl parathion pesticide","authors":"Andualem Ejigu , Molla Tefera , Adisie Kassa , Atnafu Guadie","doi":"10.1016/j.sbsr.2025.100938","DOIUrl":"10.1016/j.sbsr.2025.100938","url":null,"abstract":"<div><div>Methyl parathion (MP) is a powerful organophosphate insecticide commonly used in agriculture, but it poses serious risks to both human health and environmental safety. This study presents a highly sensitive electrochemical sensor development based on a glassy carbon electrode (GCE) that has been modified in sequence with Ethylenediaminetetraacetic acid (EDTA) and electropolymerized with poly(Alizarin Red S) (poly(ARS)) for detecting MP. The poly(ARS)/EDTA/GCE was characterized with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The EDTA improves the surface functionality and adhesion, while the redox-active poly(ARS) film aids in electron transfer and offers selective sites for MP interaction. The sensor showed significantly better stability, catalytic performance, and electroactive surface area compared to the unmodified electrode.</div><div>Under optimized pH of 7.0, the sensor reached a limit of detection (LOD) of 0.5527 μM, a limit of quantification (LOQ) of 1.8425 μM, and a linear range from 0.05 to 140 μM, with a sensitivity of 0.1265 μA/μM. It exhibited excellent analytical capabilities, such as repeatability with a relative standard deviation (RSD) of 2.01 %, reproducibility across five independently crafted electrodes with an RSD of 3.13 %, and operational stability over five days with an RSD of 1.76 %. The practical utility of the sensor was validated through MP recovery studies on fresh vegetable samples, which achieved recoveries ranging from 98 % to 113 %.</div><div>In summary, the developed poly(ARS)/EDTA/GCE sensor provides an effective and reliable method for monitoring MP in both environmental and food safety contexts.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"51 ","pages":"Article 100938"},"PeriodicalIF":4.9,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145734166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0