Sensing and Bio-Sensing Research最新文献_第10页

Stability insights into synthetic biology enabled biosensors: A case study with SARS-CoV2 toehold-based colorimetric sensor 对合成生物学生物传感器的稳定性见解：以SARS-CoV2为基础的比色传感器为例研究

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-08-01 DOI: 10.1016/j.sbsr.2025.100775

Swetha Mariam Stanley, Harvinder Khera

Improving medical and environmental diagnostics has become a pressing need. Synthetic biologists are steering biomolecular engineering efforts toward this objective, promising novel, cost-effective diagnostic solutions. While conventional antibody-based diagnostics are sensitive, they are slow, costly, and struggle with emerging pathogens or rare diseases. Synthetic biology's rapid design-to-production cycles offer a solution, introducing engineered gene circuits that diversify molecular detection, create dynamic sensors, and enable portable diagnostic tools. Toehold switch-based diagnostics emerge as a promising, inexpensive, rapid, and highly sensitive alternative to RT-qPCR, especially beneficial in resource-limited regions. These devices, adaptable to paper-based platforms, offer potential for widespread use in low-resource settings. Ensuring stability and functionality under varying environmental factors poses a challenge in their practical implementation for diagnostic purposes. To address this, our study focuses on preserving cell fee expression systems under extended temperature stress through lyophilization. Lyophilization emerges as a crucial method, potentially ensuring prolonged stability and convenient transportation of diagnostic components. We emphasize the significance of choosing the appropriate lyoprotectant, underscoring the necessity of exploring various lyoprotectants to ensure scalability and cost-effectiveness in these molecular tools. Our demonstration of dextran's practical utility in enhancing the stability of lyophilized cell-free expression system for colorimetric diagnostics, especially in detecting synthetic triggers for SARS-CoV-2, signifies a promising advancement in molecular diagnostics for resource-limited settings.

改善医疗和环境诊断已成为迫切需要。合成生物学家正在引导生物分子工程努力实现这一目标，有希望的新颖，具有成本效益的诊断解决方案。虽然传统的基于抗体的诊断方法很敏感，但它们速度慢、成本高，而且难以应对新出现的病原体或罕见疾病。合成生物学的快速设计到生产周期提供了一种解决方案，引入了工程基因电路，使分子检测多样化，创建动态传感器，并使便携式诊断工具成为可能。基于支点开关的诊断作为RT-qPCR的一种有前景、廉价、快速和高度敏感的替代方法出现，在资源有限的地区尤其有益。这些设备适用于纸质平台，有可能在资源匮乏的环境中广泛使用。确保在不同环境因素下的稳定性和功能性对其用于诊断目的的实际实施提出了挑战。为了解决这个问题，我们的研究重点是通过冻干来保存延长温度胁迫下的细胞费用表达系统。冻干成为一种重要的方法，有可能确保诊断成分的长期稳定性和方便运输。我们强调选择合适的lyo保护剂的重要性，强调探索各种lyo保护剂的必要性，以确保这些分子工具的可扩展性和成本效益。我们展示了葡聚糖在提高冻干无细胞表达系统的稳定性方面的实用性，特别是在检测SARS-CoV-2的合成触发物方面，这标志着在资源有限的环境下分子诊断方面的一个有希望的进步。

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引用次数: 0

Molybdenum diselenide-based optical fiber biosensors for targeted detection of lung cancer-associated DNA 基于二硒钼的光纤生物传感器用于肺癌相关DNA的靶向检测

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-08-01 DOI: 10.1016/j.sbsr.2025.100859

Hamed Taheri , Maryam Nayeri , Navid Nasirizadeh , Seyed Morteza Seifati , Fatemeh Ostovari

The emergence of molybdenum diselenide as a nanomaterial with unique electronic, mechanical, chemical, and optical properties has significantly advanced research in various biomedical sciences and the development of biosensors for biomolecule analysis. This research introduces a molybdenum diselenide-based biosensor to identify DNA molecules related to lung cancer. To stabilize single-stranded DNA containing a thiol group on the optical fiber surface, key parameters in the biosensor, such as stability time, solution concentration, and input power for single-stranded DNA molecules, were optimized. The hybridization process and the time required for it were evaluated, and the effect of the target molecule quantity on the limit of detection was analyzed. The results from the biosensor showed a significant increase in the optical refractive index in the optical fiber with two complementary strands compared to a single strand. In contrast, the hybridization process with non-complementary strands indicated minimal effect. The sensor demonstrated high effectiveness and selectivity in identifying single strands of DNA. Analytical properties such as linearity, detection limit, and repeatability were assessed for the designed sensor. The results indicate that it can detect concentrations from 1000 nM to 0.07 nM, with a detection limit of 88.58 pM. Its response speed is just one second, and it outperforms other sensors. Identification, early diagnosis, advanced awareness, and follow-up of lung cancer are crucial factors that can significantly facilitate treatment.

二硒化钼作为一种具有独特的电子、机械、化学和光学特性的纳米材料的出现，极大地推动了各种生物医学科学的研究和用于生物分子分析的生物传感器的发展。本研究介绍了一种基于二硒化钼的生物传感器，用于识别与肺癌相关的DNA分子。为了稳定光纤表面含有巯基的单链DNA，优化了生物传感器中的关键参数，如稳定时间、溶液浓度和单链DNA分子的输入功率。评价了杂交过程和所需时间，分析了目标分子数量对检出限的影响。生物传感器的结果表明，与单线相比，具有两条互补线的光纤的光学折射率显着增加。相反，与非互补链杂交过程显示最小的影响。该传感器在识别单链DNA方面具有很高的有效性和选择性。对所设计的传感器进行了线性度、检出限和重复性等分析性能评估。结果表明，该方法可检测浓度为1000 nM ~ 0.07 nM，检出限为88.58 pM。它的响应速度只有一秒，比其他传感器性能更好。肺癌的识别、早期诊断、提高意识和随访是显著促进治疗的关键因素。

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引用次数: 0

Tunable electric split-ring resonator with multi-functional modulation characteristics 具有多功能调制特性的可调谐电分裂环谐振器

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-08-01 DOI: 10.1016/j.sbsr.2025.100857

Wenqing Zheng, Daoye Zheng, Yunche Zhu, Tingwei Wang, Yu-Sheng Lin

This study presents an electrostatically tunable electric split-ring resonator (eSRR) design to investigate its applications in optical modulations and environmental monitors. The eSRR consists of four meta-atoms (MAs) that are centrally symmetric and free to move horizontally and vertically. Each MA is composed of a L-shaped metal structure on silicon nitride (Si₃N₄) layer. By changing the gap between MAs in x-axis (d_x) and y-axis directions (d_y), and height (h) of MAs, the active switching and dynamic modulating behaviors of seven distinct resonance modes are achieved in the eSRR design. The MAs exhibit translational displacement along individual x- and y-axes, as well as x-y planar motion simultaneously. The eSRR demonstrates broadband frequency tuning with 0.41 THz spectral shift via x-axis displacement in TE mode, while y-axis displacement enables dual-band optical switching at 0.54 THz and 0.88 THz in TM mode. Along the vertical direction, the optical properties of the eSRR are systematically investigated by lifting a single MA, two diagonally arranged MAs, and two adjacent MAs. Through changing the h value, eSRR exhibits the switching of single-, dual-, and tri-band resonance characteristics, and reaches the 0.69 resonant intensity in different resonant modes. As the environmental refractive index varies from 1.0 to 2.0, the eSRR demonstrates distinct electromagnetic responses depending on its geometrical parameters. When d_x = 20 μm, d_y = 36 μm, and h = 0 μm, the eSRR shows the maximum sensitivity of 126 GHz/RIU. These results indicate that the proposed eSRR configuration is promising for applications in optical filtering, switching, modulating, biosensing, and environmental monitoring fields.

本研究提出一种静电可调谐电分裂环谐振器（eSRR）设计，以探讨其在光调制和环境监测中的应用。eSRR由四个中心对称的元原子（MAs）组成，它们可以自由地水平和垂直移动。每个MA由氮化硅（Si3N4）层上的l形金属结构组成。通过改变MAs在x轴方向（dx）和y轴方向（dy）上的间隙以及MAs的高度(h)，在eSRR设计中实现了七种不同谐振模式的主动开关和动态调制行为。MAs沿单个x轴和y轴表现出平移位移，同时也表现出x-y平面运动。在TE模式下，eSRR通过x轴位移实现了0.41太赫兹的宽带频率调谐，而在TM模式下，y轴位移实现了0.54太赫兹和0.88太赫兹的双频光开关。在垂直方向上，通过提升单个MAs，两个对角线排列的MAs和两个相邻的MAs，系统地研究了eSRR的光学特性。通过改变h值，eSRR表现出单、双、三波段共振特性的切换，在不同的谐振模式下达到0.69的共振强度。当环境折射率从1.0到2.0变化时，eSRR表现出不同的电磁响应，这取决于其几何参数。当dx = 20 μm, dy = 36 μm, h = 0 μm时，eSRR的最大灵敏度为126 GHz/RIU。这些结果表明，所提出的eSRR结构在光滤波、开关、调制、生物传感和环境监测等领域具有广阔的应用前景。

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引用次数: 0

Corrigendum to “Graphene based Mo6 cluster hybrid for detecting simulant nerve agent DMMP” [Sensing and Bio-Sensing Research, Volume 43, February 2024, 100603] “基于石墨烯的Mo6簇混合检测模拟神经毒剂DMMP”的勘误表[传感和生物传感研究，第43卷，2024年2月，100603]

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-08-01 DOI: 10.1016/j.sbsr.2025.100863

Juan Casanova-Chafer , Xavier Blanch , Ana Moreno , Rocio Garcia-Aboal , Pedro Atienzar , Eduard Llobet , Marta Feliz

引用次数: 0

Corrigendum to “Surface acoustic waves (SAW) sensor for the active detection of Microcystin-LR (Cyanobacteria)” [Sensing and Bio-Sensing Research, Volume 47, February 2025, 100724] “用于主动检测微囊藻素- lr（蓝藻）的表面声波（SAW）传感器”的勘误表[传感和生物传感研究，第47卷，2025年2月，100724]

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-08-01 DOI: 10.1016/j.sbsr.2025.100868

Debdyuti Mandal , Tally Bovender , Robert D. Geil , Debabrata Sahoo , Sourav Banerjee

引用次数: 0

Corrigendum to “Electrochemical modulation of tau protein phosphorylation levels with biomimetic polyaniline membranes” [Sensing and Bio-Sensing Research, Volume 48, June 2025, 100805] “用仿生聚苯胺膜电化学调节tau蛋白磷酸化水平”的更正[传感和生物传感研究，第48卷，2025年6月，100805]

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-08-01 DOI: 10.1016/j.sbsr.2025.100832

Shang-Chi Chien , Chia-Hsuan Chang , Hung-Ju Wang , Chien-Chih Hsu , Yu-Lin Wang , Ching-Cheng Chuang , Jung-Chih Chen

引用次数: 0

Toward the development of a square wave voltammetry technique for in situ soil organic carbon quantification 方波伏安法在原位土壤有机碳定量中的应用

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-07-26 DOI: 10.1016/j.sbsr.2025.100856

Matthew Myers , Praveen , Stuart Watt , Wesley Moss , Joanne Wisdom

Many strategies are being developed and implemented to increase the soil carbon stock; however, demonstrating efficacy is often limited by the time and costs associated with soil sampling/analysis and a lack of real-time data to understand soil carbon spatiotemporal dynamics. As such, the development of real time in-ground sensors for monitoring changes in soil organic carbon over time is necessary to validate (or invalidate) these strategies in the real world. In situ near-IR spectroscopic techniques have been developed for quantifying and characterizing organic carbon in soil. This strategy is strongly affected by soil moisture content, relies on complex statistical and machine learning techniques and is relatively expensive. In this work, we examine the feasibility of developing a low-cost electrochemical technique for the quantification of soluble soil carbon species in soil. Given that these species are very diverse, commercially available fulvic/humic acid is used as a pathway for sensor development. Using a composite film consisting of a poly(methyl methacrylate) resin (to attract fulvic/humic acid to the electrode surface) and a hydrogel material (to bring moisture to the electrode surface), a linear response over a fulvic acid concentration range 0 to 0.05 wt% has been demonstrated. Multi-day measurements in a varying temperature environment have shown that there is a strong correlation between response and temperature. By implementing these correlations and optimizing the coating characteristics, we show that the sensor has a lifetime of over one month in aqueous solution.

正在制定和实施许多战略以增加土壤碳储量；然而，证明其有效性往往受到与土壤采样/分析相关的时间和成本的限制，并且缺乏了解土壤碳时空动态的实时数据。因此，开发用于监测土壤有机碳随时间变化的实时地下传感器对于在现实世界中验证（或无效）这些策略是必要的。原位近红外光谱技术已被用于土壤中有机碳的定量和表征。这种策略受到土壤含水量的强烈影响，依赖于复杂的统计和机器学习技术，并且相对昂贵。在这项工作中，我们研究了开发一种低成本的电化学技术来定量土壤中可溶性土壤碳的可行性。鉴于这些物种非常多样化，商用黄腐酸/腐植酸被用作传感器开发的途径。使用由聚（甲基丙烯酸甲酯）树脂（将富里酸/腐植酸吸引到电极表面）和水凝胶材料（将水分带到电极表面）组成的复合膜，在富里酸浓度范围0至0.05 wt%的线性响应已被证明。在不同温度环境中进行的多日测量表明，反应与温度之间存在很强的相关性。通过实现这些相关性并优化涂层特性，我们表明传感器在水溶液中的使用寿命超过一个月。

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引用次数: 0

Determination of copper ions at the sediment-seawater interface through ion-selective microelectrode based on current pulse 基于电流脉冲的离子选择微电极测定沉积物-海水界面的铜离子

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-07-25 DOI: 10.1016/j.sbsr.2025.100858

Jiali Zhai , Yixin Hu , Mingkang Li , Hongwei Liu , Xin Liang , Jie Rong , Guangtao Zhao

The chronopotentiometric measurements based on ion-selective microelectrode (ISμE) could be a promising tool for the detection of free copper ion in seawater at the sediment-water interface with high sensitivity. In this work, an all-solid-contact copper ion-selective microelectrode (Cu²⁺-ISμE) based on platinum wire was designed for chronopotentiometric determination of copper in seawater at the sediment-water interface. The lipophilic salt ETH 500 was added into the Cu²⁺-selective membrane instead of traditional ion-exchanger, and the extraction of copper could be galvanostatically controlled. The present chronopotentiometric method shows a linear activity range of 2.5 × 10⁻⁴ - 2.5 × 10⁻⁸ M with a slope of 34.75 ± 0.5 mV/decade under the optimized conditions, and the detection limit is 5.8 × 10⁻⁹ M. Moreover, the chronopotentiometric method has a good selectivity and reproductivity. Additionally, the feasibility of the Cu²⁺-ISμE has been investigated for the measurement of the content of Cu²⁺ in the seawater samples at the sediment-water interface with a small volume (e.g., 1 mL) without any pretreatment, and the spike recovery for this method is from 92.24 % to 106.51 %. Herein, the detection of copper through the pulse current based Cu²⁺-ISμE has satisfactory results. The galvanostatic controlled technique based on ISμE has potential application in the in-situ determination of trace heavy metals in seawater.

基于离子选择微电极（ISμE）的时间电位测量方法具有较高的灵敏度，有望成为沉积物-水界面海水中游离铜离子检测的一种有前景的工具。本文设计了一种基于铂丝的全固体接触铜离子选择性微电极（Cu2+-ISμE），用于沉积物-水界面处海水中铜的计时电位测定。在Cu2+选择性膜中加入亲脂盐ETH 500代替传统的离子交换剂，铜的萃取可以通过恒流控制。在优化条件下，时间电位法的线性活度范围为2.5 × 10−4 ~ 2.5 × 10−8 M，斜率为34.75±0.5 mV/ 10年，检出限为5.8 × 10−9 M，具有良好的选择性和重复性。此外，还研究了Cu2+-ISμE在沉积物-水界面小体积（如1 mL）海水样品中不经任何预处理的Cu2+含量测定的可行性，该方法的峰回收率为92.24% ~ 106.51%。基于脉冲电流的Cu2+-ISμE对铜的检测取得了满意的结果。基于ISμE的恒流控制技术在海水中痕量重金属的原位测定中具有潜在的应用前景。

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引用次数: 0

Triple-synergistic Mn-MOF electrochemical sensor for multiplex antibiotic detection: A robust and stable platform for food safety monitoring 用于多种抗生素检测的三协同Mn-MOF电化学传感器：一个鲁棒稳定的食品安全监测平台

IF 4.9 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-07-23 DOI: 10.1016/j.sbsr.2025.100854

Sumei Li , Bo Yan , Lulu Liu , Haiying Yang , Chengyu Zhang , Jianli Shi , Jing Su , Wenjuan Ji

The excessive use of nitrofurantoin (NFT), nitrofurazone (NFZ), tetracycline hydrochloride (TC), and chloramphenicol (CAP) poses significant health risks due to their toxicity. Existing detection methods are limited in sensitivity and portability, which hinders rapid on-site monitoring of antibiotic residues contemporary livestock production. This study introduces an electrochemical sensor based on {[Mn₂(BPTC)(DMA)₂(H₂O)₂]·DMA}_n (SXNU-2-Mn) (H₄BPTC = biphenyl-3,3′,5,5′-tetracarboxylic acid, DMA = N, N-Dimethylacetamide, SXNU = Shanxi Normal University). The Metal-organic frameworks (MOFs) SXNU-2-Mn features a three-dimensional PtS topology with a unique pore microenvironment containing hydroxyl oxygen groups and DMA molecules, along with its manganese (Mn) catalytic center. The rod-shaped nanometer array of pristine SXNU-2-Mn/GCE sensor demonstrates exceptional performance in detecting and differentiating multiple antibiotics, including NFT, NFZ, TC, and CAP, each with distinct redox potential. Notably, for CAP, the sensor exhibits an extensive linear range from 0.07 to 900.00 μM and an exceptionally much lower limit of detection (LOD) of 19.45 nM. Stability assessments indicate that the sensor retains over 94.6 % of its initial performance after seven days, with a variation of less than 2.4 % for CAP detection. The pore environment facilitates a triple-synergistic smart recognition mechanism for CAP, involving π-π stacking, hydrogen bonding, and Mn(II)-mediated catalytic interactions. The practical applicability of the SXNU-2-Mn/GCE sensor was validated through real-world food samples such as chicken and milk, achieving recovery rates ranging from 95.45 % to 108.10 %. This research presents a pristine MOF platform that integrates molecular recognition and redox catalysis, offering a portable solution for simultaneous antibiotic residue detection and advancing food safety monitoring technologies.

呋喃妥因（NFT）、呋喃酮（NFZ）、盐酸四环素（TC）和氯霉素（CAP）的过量使用因其毒性而构成重大健康风险。现有的检测方法在灵敏性和便携性方面存在局限性，阻碍了对当代畜牧生产中抗生素残留的快速现场监测。本研究介绍了一种基于{[Mn2(BPTC)(DMA)2(H2O)2]·DMA}n （SXNU-2- mn）的电化学传感器（H4BPTC =联苯-3,3 ',5,5 ' -四羧酸，DMA = n， n -二甲基乙酰胺，SXNU =山西师范大学）。金属有机框架（mof） SXNU-2-Mn具有三维PtS拓扑结构，具有独特的孔隙微环境，含有羟基氧基团和DMA分子，以及锰（Mn）催化中心。原始SXNU-2-Mn/GCE传感器的棒状纳米阵列在检测和区分多种抗生素方面表现出卓越的性能，包括NFT， NFZ， TC和CAP，每种抗生素都具有不同的氧化还原电位。值得注意的是，对于CAP，该传感器具有0.07至900.00 μM的广泛线性范围，并且检测限（LOD）非常低，为19.45 nM。稳定性评估表明，传感器在7天后保持了超过94.6%的初始性能，CAP检测的变化小于2.4%。孔隙环境促进了CAP的三协同智能识别机制，包括π-π堆叠、氢键和Mn（II）介导的催化相互作用。通过鸡肉和牛奶等实际食品样品验证了SXNU-2-Mn/GCE传感器的实用性，回收率为95.45% ~ 108.10%。本研究提出了一个集分子识别和氧化还原催化于一体的原始MOF平台，为同时检测抗生素残留提供了便携式解决方案，推动了食品安全监测技术的发展。

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引用次数: 0

Metal-organic framework-based aptamer sensors for early diagnosis of breast cancer 基于金属有机框架的适体传感器在乳腺癌早期诊断中的应用

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-07-23 DOI: 10.1016/j.sbsr.2025.100855

Xiaoxia Yao , Wei Chen , Chi Zhang , Weixuan Shao , Mohammadreza Shokouhimehr , Can Guo , Zhengchun Liu

Breast cancer is among the most common neoplasms in women worldwide, and timely detection is important to ensure the greatest chance of survival. Metal-organic frameworks (MOFs) possess specific advantages for application in biosensors because they possess a high specific surface area, tunable pore structure, and excellent biocompatibility. This paper summarizes the research progress of MOF-based aptamer sensors for the early detection of biomarkers of breast cancer, including progress in single-biomarker and multiplex sensing and the use of various detection methods in aptamer-based sensors. Furthermore, it discusses novel component design, optimization of detection methods, and the expansion of clinical applications with MOF-based aptamer sensors. Functionalization and adjustment of MOFs through the introduction of new aptamers and techniques have improved sensor efficiency and provided new paths for early cancer diagnosis. Despite advancements, sensitivity, selectivity, and clinical feasibility concerns are areas that require more efforts.

乳腺癌是全世界女性中最常见的肿瘤之一，及时发现对于确保最大的生存机会至关重要。金属有机骨架（mof）具有高比表面积、可调节的孔结构和优异的生物相容性，在生物传感器中具有特殊的应用优势。本文综述了基于mof的适体传感器用于乳腺癌生物标志物早期检测的研究进展，包括单生物标志物和多生物标志物检测的进展以及各种检测方法在适配体传感器中的应用。此外，它还讨论了新的组件设计，检测方法的优化以及基于mof的适体传感器的临床应用的扩展。通过引入新的适配体和技术对mof进行功能化和调整，提高了传感器效率，为早期癌症诊断提供了新的途径。尽管取得了进步，但敏感性、选择性和临床可行性方面的问题仍需要更多的努力。

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引用次数: 0