Sensing and Bio-Sensing Research最新文献_第2页

On-Chip DO sensor based on phosphorescence lifetime spectroscopy for investigation of activation parameters in photodynamic therapy of cancers 基于磷光寿命光谱的片上溶解氧传感器，用于研究癌症光动力疗法中的活化参数

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-03-22 DOI: 10.1016/j.sbsr.2025.100779

E. Heydari , S. Delavari , K. Hajisharifi , S. Emadi , H. Radnaseri , M. Moeini , H. Mehdian , A. Erdem , G. Bai , M. Razzaghi

A 3D-printed optofluidic chip with an embedded dissolved oxygen sensor, empowered with a time-resolved phosphorescence lifetime spectroscopy platform, is developed for indirect analysis of reactive oxygen species (ROS) dynamics in photodynamic therapy (PDT). This platform is implemented for continuous evaluation of ROS production/elimination through real-time measurement of photoluminescence (PL) lifetime (

τ

) during PDT treatment, revealing the key contributions of the photosensitizer (PS), excitation laser, and the medium in ROS generation during this process. Rose Bengal (RB) is utilized as a PS to demonstrate this system's capability to analyze and tune the PS activation parameters such as PS concentration, laser exposure time, and power. In addition, the platform provides important information on the medium activation duration, the maximum changes in the PL lifetime (

∆ τ

_max), and the time to reach

∆ τ

_max. For the Dulbecco's modified eagle medium high glucose (DMEM HG) containing fetal bovine serum (FBS), RB, and A375 human melanoma cell line as a representative example, these parameters are 1070 s, 4.4 μs, and 780 s respectively. Two ROS scavengers of sodium pyruvate (SP) and terephthalic acid (TA) are used to demonstrate that more than 90 % of the change in the

τ

corresponds to OH• and H₂O₂ radicals, confirming the correlation between ROS generation/elimination and

τ

variations. Moreover, this system is compared with conventional absorption and photoluminescent methods based on 1,3-diphenylisobenzofuran (DPBF) indicator. Unlike DPBF and similar indicators, this on-chip system besides providing real-time data on the dynamics of activation and deactivation of the PSs, enables distinguishing the contribution of various parameters, and is not consumed during the measurement and can be reused multiple times. Therefore, the developed platform is potentially beneficial for on-chip drug analysis and development in PDT therapy, as well as other biomedical applications.

{"title":"On-Chip DO sensor based on phosphorescence lifetime spectroscopy for investigation of activation parameters in photodynamic therapy of cancers","authors":"E. Heydari , S. Delavari , K. Hajisharifi , S. Emadi , H. Radnaseri , M. Moeini , H. Mehdian , A. Erdem , G. Bai , M. Razzaghi","doi":"10.1016/j.sbsr.2025.100779","DOIUrl":"10.1016/j.sbsr.2025.100779","url":null,"abstract":"<div><div>A 3D-printed optofluidic chip with an embedded dissolved oxygen sensor, empowered with a time-resolved phosphorescence lifetime spectroscopy platform, is developed for indirect analysis of reactive oxygen species (ROS) dynamics in photodynamic therapy (PDT). This platform is implemented for continuous evaluation of ROS production/elimination through real-time measurement of photoluminescence (PL) lifetime (<span><math><mi>τ</mi></math></span>) during PDT treatment, revealing the key contributions of the photosensitizer (PS), excitation laser, and the medium in ROS generation during this process. Rose Bengal (RB) is utilized as a PS to demonstrate this system's capability to analyze and tune the PS activation parameters such as PS concentration, laser exposure time, and power. In addition, the platform provides important information on the medium activation duration, the maximum changes in the PL lifetime (<span><math><mo>∆</mo><mi>τ</mi></math></span><sub>max</sub>), and the time to reach <span><math><mo>∆</mo><mi>τ</mi></math></span><sub>max</sub>. For the Dulbecco's modified eagle medium high glucose (DMEM HG) containing fetal bovine serum (FBS), RB, and A375 human melanoma cell line as a representative example, these parameters are 1070 s, 4.4 μs, and 780 s respectively. Two ROS scavengers of sodium pyruvate (SP) and terephthalic acid (TA) are used to demonstrate that more than 90 % of the change in the <span><math><mi>τ</mi></math></span> corresponds to OH• and H<sub>2</sub>O<sub>2</sub> radicals, confirming the correlation between ROS generation/elimination and <span><math><mi>τ</mi></math></span> variations. Moreover, this system is compared with conventional absorption and photoluminescent methods based on 1,3-diphenylisobenzofuran (DPBF) indicator. Unlike DPBF and similar indicators, this on-chip system besides providing real-time data on the dynamics of activation and deactivation of the PSs, enables distinguishing the contribution of various parameters, and is not consumed during the measurement and can be reused multiple times. Therefore, the developed platform is potentially beneficial for on-chip drug analysis and development in PDT therapy, as well as other biomedical applications.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100779"},"PeriodicalIF":5.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative study of convolutional neural networks and traditional feature extraction techniques for adulteration detection in ground beef

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-03-21 DOI: 10.1016/j.sbsr.2025.100774

Leila Bahmani , Saied Minaei , Alireza Mahdavian , Ahmad Banakar , Mahmoud Soltani Firouz

The presence of adulteration in meat products, including minced meat, is a serious concern in many parts of the world. Therefore, notable efforts have been made to find fast, non-destructive and efficient methods to detect adulteration in minced meat. In this research, thermal imaging was investigated to detect adulteration of ground beef in two data sets that included sheep lung and chicken gizzard as impurities. In order to identify the most appropriate feature extraction algorithm and classify samples having various levels of adulteration, Local Binary Pattern (LBP), Gray Level Co-occurrence Matrixes (GLCM) and Gabor filter were compared. Convolutional Neural Network (CNN) was also used to extract features and classify images. In order to evaluate these algorithms, the following criteria were utilized: accuracy, precision, recall, specificity and F-score. Results showed that for both datasets, the best performance was obtained using the Gabor filter while the weakest performance was related to the LBP algorithm. However, CNN, with a total accuracy of over 99 % in both data sets, was found to surpass the other methods and is recommended as the best approach for analyzing thermal images of ground beef adulterated with avian and ovine offal. This shows the superiority of CNN algorithm over machine learning algorithms in identifying adulteration in minced meat. The experimental results and the associated data analysis presented here show the appropriate use of thermography in identifying meat fraud, which can be suitable for online applications.

{"title":"A comparative study of convolutional neural networks and traditional feature extraction techniques for adulteration detection in ground beef","authors":"Leila Bahmani , Saied Minaei , Alireza Mahdavian , Ahmad Banakar , Mahmoud Soltani Firouz","doi":"10.1016/j.sbsr.2025.100774","DOIUrl":"10.1016/j.sbsr.2025.100774","url":null,"abstract":"<div><div>The presence of adulteration in meat products, including minced meat, is a serious concern in many parts of the world. Therefore, notable efforts have been made to find fast, non-destructive and efficient methods to detect adulteration in minced meat. In this research, thermal imaging was investigated to detect adulteration of ground beef in two data sets that included sheep lung and chicken gizzard as impurities. In order to identify the most appropriate feature extraction algorithm and classify samples having various levels of adulteration, Local Binary Pattern (LBP), Gray Level Co-occurrence Matrixes <strong>(</strong>GLCM) and Gabor filter were compared. Convolutional Neural Network (CNN) was also used to extract features and classify images. In order to evaluate these algorithms, the following criteria were utilized: accuracy, precision, recall, specificity and F-score. Results showed that for both datasets, the best performance was obtained using the Gabor filter while the weakest performance was related to the LBP algorithm. However, CNN, with a total accuracy of over 99 % in both data sets, was found to surpass the other methods and is recommended as the best approach for analyzing thermal images of ground beef adulterated with avian and ovine offal. This shows the superiority of CNN algorithm over machine learning algorithms in identifying adulteration in minced meat. The experimental results and the associated data analysis presented here show the appropriate use of thermography in identifying meat fraud, which can be suitable for online applications.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100774"},"PeriodicalIF":5.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fiber laser generation of an ag/MoS2/PVP nanoparticle composite SERS sensor for benzene detection in water

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-03-21 DOI: 10.1016/j.sbsr.2025.100780

Rana M. Taha , Fatema H. Rajab , Alaa Al-Taie

One of the most widely used practices to detect chemical contaminants at ultra-low concentrations is Surface-Enhanced Raman Scattering (SERS), a highly sensitive technique that provides the unmatched sensitivity necessary for environmental and safety applications. In this work, we report the synthesis of a silver (Ag), molybdenum disulfide (MoS₂) and polyvinylpyrrolidone (PVP) nanoparticle composite by fiber laser ablation in water to produce a high-performance SERS sensor by adapting both electromagnetic (EM) and chemical mechanism (CM) in a composite. The CM enhancement originates from MoS₂ NPs charge transfer whereas the EM enhancement associated with localized surface plasmon resonance of Ag NP. Crystal violet (CV) was utilized as a probe molecule for evaluating the performance of the SERS sensor so produced. It was then utilized in the detection of trace amounts of benzene in DI, tape and river water with a maximum enhancement factor (EF) of 3 × 10⁷ and a detection limit up to 0.001 nM confirming its high sensitivity, reliability and long-time stability as well as its potential as an ideal monitoring platform for benzene pollution.

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引用次数: 0

Construction and optimization of a versatile bioFET platform for detecting nucleic acids and protein using the biotin–neutravidin affinity system

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-03-18 DOI: 10.1016/j.sbsr.2025.100778

Jiunn-Tyng Yeh , Lian-Chin Wang , Chi-Pei Weng , Chang-Fu Kuo

A biosensor based on a field-effect transistor (bioFET) is a promising system for point-of-care testing due to its portability and high sensitivity. A bioFET uses a linker platform to connect capture molecules to the FET, which detects electrical changes when targets bind to the capture molecules. This study presents the development and optimization of a versatile, highly sensitive bioFET platform utilizing a biotin–neutravidin affinity system capable of detecting both DNA and proteins. The bioFET was constructed using an extended-gate field-effect transistor (EGFET) with an aluminum gate functionalized with (3-aminopropyl)triethoxysilane (APTES)–biotin–neutravidin. We optimized the concentrations and molar ratio of biotin and neutravidin using fluorescence imaging and biosensing benchmarks. We found that 0.1 μg/mL biotin paired with 30 μg/mL neutravidin, approximately 1:1 in molarity, achieved optimal sensitivity. The optimized bioFET system demonstrated good sensitivity, with lower detection limits of 3.5 copies for Escherichia coli genomic DNA and 0.3 fg/mL for p-Tau217, an Alzheimer's disease biomarker. In summary, this study establishes the ideal conditions for constructing a bioFET system using an APTES–biotin–neutravidin linker capable of detecting DNA and proteins, with potential for convenient and sensitive point-of-care diagnostics.

{"title":"Construction and optimization of a versatile bioFET platform for detecting nucleic acids and protein using the biotin–neutravidin affinity system","authors":"Jiunn-Tyng Yeh , Lian-Chin Wang , Chi-Pei Weng , Chang-Fu Kuo","doi":"10.1016/j.sbsr.2025.100778","DOIUrl":"10.1016/j.sbsr.2025.100778","url":null,"abstract":"<div><div>A biosensor based on a field-effect transistor (bioFET) is a promising system for point-of-care testing due to its portability and high sensitivity. A bioFET uses a linker platform to connect capture molecules to the FET, which detects electrical changes when targets bind to the capture molecules. This study presents the development and optimization of a versatile, highly sensitive bioFET platform utilizing a biotin–neutravidin affinity system capable of detecting both DNA and proteins. The bioFET was constructed using an extended-gate field-effect transistor (EGFET) with an aluminum gate functionalized with (3-aminopropyl)triethoxysilane (APTES)–biotin–neutravidin. We optimized the concentrations and molar ratio of biotin and neutravidin using fluorescence imaging and biosensing benchmarks. We found that 0.1 μg/mL biotin paired with 30 μg/mL neutravidin, approximately 1:1 in molarity, achieved optimal sensitivity. The optimized bioFET system demonstrated good sensitivity, with lower detection limits of 3.5 copies for <em>Escherichia coli</em> genomic DNA and 0.3 fg/mL for p-Tau217, an Alzheimer's disease biomarker. In summary, this study establishes the ideal conditions for constructing a bioFET system using an APTES–biotin–neutravidin linker capable of detecting DNA and proteins, with potential for convenient and sensitive point-of-care diagnostics.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100778"},"PeriodicalIF":5.4,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143678951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microneedle-based electrochemical sensors for real-time pH and sodium monitoring in physiological environments

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-03-14 DOI: 10.1016/j.sbsr.2025.100777

Fahima Rahman , Adam Ryan , Andrea Bocchino , Paul Galvin , Sofia Rodrigues Teixeira

This study proposes developing microneedle (MN) sensors for pH and sodium detection. MNs are minimally invasive, miniaturized needles capable of piercing the stratum corneum to access dermal interstitial fluid (ISF). They can offer accessible, quick, and precise point-of-care diagnostics, potentially replacing centralized laboratory testing. The study uses electrochemical techniques for sensor modification, detection, and in-vitro characterizations. This work aimed to create and validate a polymer-based disposable microneedle patch for future transdermal electrochemical sensing. Successful potentiometric sensor development for pH detection using SiOx as passivation layers with IrOx functionalization was demonstrated. Additionally, voltametric sodium sensors were achieved with ARcare passivation and PEDOT functionalization. Both pH and Na⁺ sensors exhibited linear responses within normal physiological levels across various solutions. The pH sensors showed sensitivity of −60.5 mV/pH and an accuracy of 97.7 % alongside an error margin of 2.3 %, while sodium sensors achieved a sensitivity of 3.29 nA/mM/mm². Both sensors exhibit dynamic, rapid responses, along with good repeatability, stability, and selectivity. Over a twenty-one-day span for pH sensors and a fourteen-day period for sodium sensors, this study offers validation that microneedles serve as a viable foundation for wearable systems, enabling real-time, multiparameter biosensing of interstitial fluids.

{"title":"Microneedle-based electrochemical sensors for real-time pH and sodium monitoring in physiological environments","authors":"Fahima Rahman , Adam Ryan , Andrea Bocchino , Paul Galvin , Sofia Rodrigues Teixeira","doi":"10.1016/j.sbsr.2025.100777","DOIUrl":"10.1016/j.sbsr.2025.100777","url":null,"abstract":"<div><div>This study proposes developing microneedle (MN) sensors for pH and sodium detection. MNs are minimally invasive, miniaturized needles capable of piercing the stratum corneum to access dermal interstitial fluid (ISF). They can offer accessible, quick, and precise point-of-care diagnostics, potentially replacing centralized laboratory testing. The study uses electrochemical techniques for sensor modification, detection, and in-vitro characterizations. This work aimed to create and validate a polymer-based disposable microneedle patch for future transdermal electrochemical sensing. Successful potentiometric sensor development for pH detection using SiOx as passivation layers with IrOx functionalization was demonstrated. Additionally, voltametric sodium sensors were achieved with ARcare passivation and PEDOT functionalization. Both pH and Na<sup>+</sup> sensors exhibited linear responses within normal physiological levels across various solutions. The pH sensors showed sensitivity of −60.5 mV/pH and an accuracy of 97.7 % alongside an error margin of 2.3 %, while sodium sensors achieved a sensitivity of 3.29 nA/mM/mm<sup>2</sup>. Both sensors exhibit dynamic, rapid responses, along with good repeatability, stability, and selectivity. Over a twenty-one-day span for pH sensors and a fourteen-day period for sodium sensors, this study offers validation that microneedles serve as a viable foundation for wearable systems, enabling real-time, multiparameter biosensing of interstitial fluids.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"48 ","pages":"Article 100777"},"PeriodicalIF":5.4,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary glucose detection with spiral shape hollow core photonic crystal fiber: Towards improved diabetes management

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100748

Md. Safiul Islam , A.H.M. Iftekharul Ferdous , Md. Jakir Hossen , Abdullah Al Mamun , Khalid Sifulla Noor , Diponkar Kundu , Md. Galib Hasan

Currently, diabetes is a prevalent ailment worldwide. Insufficient insulin leads to an inability to transform glucose into energy, resulting in increased amounts of glucose in human body. Acute diabetes treatment and follow-up rely on urine glucose detection since it provides a non-invasive, reasonably priced means to assess glucose levels and regulate the progression of the disease. This article presents the development of a circular shape core and semi-circular trapezoid air holes biosensor PCF to assess the glucose concentration in urine inside the THz band. With matching RI's of 1.335, 1.336, 1.337, 1.338, 1.341, and 1.347, glucose quantity in bio-sample spans 0 to 15 mg/dl (for as usual people) and 0.625 g/dL, 1.25 g/dL, 2.5 g/dL, 5 g/dL, and 10 g/dL (for diabetic patients) and achieve highest sensitivity is 99.12 %, EML loss 0.0021 cm⁻¹, 2.24× 10⁻¹³ dB/m for 10 g/dl. The innovative design of this device guarantees precise and non-intrusive monitoring of the various stages of diabetes, allowing for timely and efficient control of the condition. This revolutionary sensor improves patient care and facilitates early intervention by offering a dependable and cost-efficient alternative to conventional approaches. Timely identification aids in averting complications and enhances patient prognosis.

{"title":"Urinary glucose detection with spiral shape hollow core photonic crystal fiber: Towards improved diabetes management","authors":"Md. Safiul Islam , A.H.M. Iftekharul Ferdous , Md. Jakir Hossen , Abdullah Al Mamun , Khalid Sifulla Noor , Diponkar Kundu , Md. Galib Hasan","doi":"10.1016/j.sbsr.2025.100748","DOIUrl":"10.1016/j.sbsr.2025.100748","url":null,"abstract":"<div><div>Currently, diabetes is a prevalent ailment worldwide. Insufficient insulin leads to an inability to transform glucose into energy, resulting in increased amounts of glucose in human body. Acute diabetes treatment and follow-up rely on urine glucose detection since it provides a non-invasive, reasonably priced means to assess glucose levels and regulate the progression of the disease. This article presents the development of a circular shape core and semi-circular trapezoid air holes biosensor PCF to assess the glucose concentration in urine inside the THz band. With matching RI's of 1.335, 1.336, 1.337, 1.338, 1.341, and 1.347, glucose quantity in bio-sample spans 0 to 15 mg/dl (for as usual people) and 0.625 g/dL, 1.25 g/dL, 2.5 g/dL, 5 g/dL, and 10 g/dL (for diabetic patients) and achieve highest sensitivity is 99.12 %, EML loss 0.0021 cm<sup>−1</sup>, 2.24× 10<sup>−13</sup> dB/m for 10 g/dl. The innovative design of this device guarantees precise and non-intrusive monitoring of the various stages of diabetes, allowing for timely and efficient control of the condition. This revolutionary sensor improves patient care and facilitates early intervention by offering a dependable and cost-efficient alternative to conventional approaches. Timely identification aids in averting complications and enhances patient prognosis.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100748"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A portable electrochemical immunosensor for detection of S. aureus based on polyelectrolyte-modified screen-printed carbon electrode

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100744

Aleksei A. Evdokimov, Anna A. Baldina, Anna A. Nikitina, Timur A. Aliev, Artemii M. Zenkin, Mikhail V. Zhukov, Igor S. Pantyukhin, Ekaterina V. Skorb , Anton A. Muravev

In this report, we present electrochemical immunosensors for the detection of S. aureus bacteria on the basis of SPCE/PEI/АB_SA/PSS layer-by-layer assembly as a recognition element. QCM measurements and AFM imaging ensure effective adhesion of S. aureus antibody to PEI surface and its strong interactions with analyte through the PSS polyelectrolyte layer. Impedimetric detection of S. aureus gives the LOD of 1000 CFU/mL and the linear range from 10⁴ to 10⁷ CFU/mL and features facile assembly of recognition element and easy sampling. Voltammetric detection of the formation of the sandwich immunocomplex with secondary antibody in the outermost layer (AB–AG–AB-HRP) not only decreases the detection limit to 230 CFU/mL and expands the linear range of detection to 10³–10⁸ CFU/mL, but also could detect S. aureus bacteria with a portable open-source custom potentiostat in voltammetric mode, which is promising for non-invasive point-of-care monitoring of pathogens and addresses issues of antibody-based sensors, such as high cost and difficult chemical modification.

{"title":"A portable electrochemical immunosensor for detection of S. aureus based on polyelectrolyte-modified screen-printed carbon electrode","authors":"Aleksei A. Evdokimov, Anna A. Baldina, Anna A. Nikitina, Timur A. Aliev, Artemii M. Zenkin, Mikhail V. Zhukov, Igor S. Pantyukhin, Ekaterina V. Skorb , Anton A. Muravev","doi":"10.1016/j.sbsr.2025.100744","DOIUrl":"10.1016/j.sbsr.2025.100744","url":null,"abstract":"<div><div>In this report, we present electrochemical immunosensors for the detection of <em>S. aureus</em> bacteria on the basis of SPCE/PEI/АB<sub><em>SA</em></sub>/PSS layer-by-layer assembly as a recognition element. QCM measurements and AFM imaging ensure effective adhesion of <em>S. aureus</em> antibody to PEI surface and its strong interactions with analyte through the PSS polyelectrolyte layer. Impedimetric detection of <em>S. aureus</em> gives the LOD of 1000 CFU/mL and the linear range from 10<sup>4</sup> to 10<sup>7</sup> CFU/mL and features facile assembly of recognition element and easy sampling. Voltammetric detection of the formation of the sandwich immunocomplex with secondary antibody in the outermost layer (AB–AG–AB-HRP) not only decreases the detection limit to 230 CFU/mL and expands the linear range of detection to 10<sup>3</sup>–10<sup>8</sup> CFU/mL, but also could detect <em>S. aureus</em> bacteria with a portable open-source custom potentiostat in voltammetric mode, which is promising for non-invasive point-of-care monitoring of pathogens and addresses issues of antibody-based sensors, such as high cost and difficult chemical modification.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100744"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemiluminescence ratio sensor for detecting MCP-1 based on s-PdNS

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2024.100723

Weiran Mao , Xiaoyan Zhang , Yuanyuan Yin , Xiaohua Tang , Qingqing Jiang , Xia Chen , Xiaoliang Chen

This study proposes a novel sandwich-based ratio electrochemiluminescence (ECL) immunosensor for detecting monocyte chemotactic protein-1 (MCP-1), a marker of cardiovascular disease. We synthesized sulfur-doped palladium nanosheets (S-PdNS), which possess a stable structure and uniform morphology, and can quench signals generated by the S₂O₈²⁻/O₂ ECL system. Using glutaraldehyde (GA), we conjugated luminol to these nanosheets and combined them with a secondary antibody. This modification enabled the nanomaterial to produce a new luminol signal as an internal reference for detection while quenching the S₂O₈²⁻/O₂ ECL signal. To date, no studies have utilized both mechanisms for detection. Furthermore, the deposition of gold nanoparticles (AuNPs) on the surface of the glassy carbon electrode (GCE) allowed for increased antibody binding and improved electron transmission in the ECL system. When trace analytes are present, the S₂O₈²⁻/O₂ ECL signal decreases, and the luminol ECL signal increases. This setup allowed us to obtain two signals from one measurement and use the ratio to construct a standard curve, significantly reducing the possibility of misjudgment. Under optimal conditions, the linear detection range for MCP-1 is 10^–2.5 pg mL⁻¹ to 10³ pg mL⁻¹, with a detection limit of 1.6 fg mL⁻¹ (S / N = 3). The immunosensor also exhibited excellent selectivity, repeatability, and stability, expanding the application of the dual luminescence mechanism and providing an effective method for early monitoring of cardiovascular disease.

{"title":"Electrochemiluminescence ratio sensor for detecting MCP-1 based on s-PdNS","authors":"Weiran Mao , Xiaoyan Zhang , Yuanyuan Yin , Xiaohua Tang , Qingqing Jiang , Xia Chen , Xiaoliang Chen","doi":"10.1016/j.sbsr.2024.100723","DOIUrl":"10.1016/j.sbsr.2024.100723","url":null,"abstract":"<div><div>This study proposes a novel sandwich-based ratio electrochemiluminescence (ECL) immunosensor for detecting monocyte chemotactic protein-1 (MCP-1), a marker of cardiovascular disease. We synthesized sulfur-doped palladium nanosheets (S-PdNS), which possess a stable structure and uniform morphology, and can quench signals generated by the S<sub>2</sub>O<sub>8</sub><sup>2−</sup>/O<sub>2</sub> ECL system. Using glutaraldehyde (GA), we conjugated luminol to these nanosheets and combined them with a secondary antibody. This modification enabled the nanomaterial to produce a new luminol signal as an internal reference for detection while quenching the S<sub>2</sub>O<sub>8</sub><sup>2−</sup>/O<sub>2</sub> ECL signal. To date, no studies have utilized both mechanisms for detection. Furthermore, the deposition of gold nanoparticles (AuNPs) on the surface of the glassy carbon electrode (GCE) allowed for increased antibody binding and improved electron transmission in the ECL system. When trace analytes are present, the S<sub>2</sub>O<sub>8</sub><sup>2−</sup>/O<sub>2</sub> ECL signal decreases, and the luminol ECL signal increases. This setup allowed us to obtain two signals from one measurement and use the ratio to construct a standard curve, significantly reducing the possibility of misjudgment. Under optimal conditions, the linear detection range for MCP-1 is 10<sup>–2.5</sup> pg mL<sup>−1</sup> to 10<sup>3</sup> pg mL<sup>−1</sup>, with a detection limit of 1.6 fg mL<sup>−1</sup> (S / <em>N</em> = 3). The immunosensor also exhibited excellent selectivity, repeatability, and stability, expanding the application of the dual luminescence mechanism and providing an effective method for early monitoring of cardiovascular disease.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100723"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly efficient assessment and selective determination of pancreatic cancer biomarker CA-19-9 using co-electrodeposited MXene-AgNPs nanocomposite

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100753

Mahsa Mirzayi , Balal Khalilzadeh , Sattar Akbari Nakhjavani , Rahim Mohammad-Rezaei

An ultrasensitive and label-free electrochemical immunosensor based on MXene-AgNPs nanocomposite is reported for pancreas cancer diagnostics. Ease of deposition, high speed, thickness of deposition and high efficiency of these biosensors are their special and fundamental advantages.

For this purpose, a pencil graphite electrode (PGE) was modified by silver and MXene (Ti3C2Tx (to increase efficiency and also the active surface area of PGE. Deposition of AgNPs-MXene nanocomposite then immobilization of streptavidin and biotinylated antibody on the electrode increases the sensitivity, conductivity and stability of the designed immunosensor. The electrochemical measurements in the attendance and absence of AgNPs-MXene can causing an increase in electrical currents. To examine the morphology of the developed immunosensor for the purpose of enhancing its optimal performance, the characterization methods employed include Field Emission Scanning Electron Microscopy (FE-SEM), Fourier-Transform Infrared Spectroscopy (FT-IR), and X-ray Diffraction (XRD). The results of the studies projected antibody (Ab) CA-19-9/AgNPs-MXene electrochemical immunosensor as a potential and affordable alternate of conventional methods like ELISA. The desirable LOD was recorded as 1 nU/mL. The repeatability and reproducibility were successfully inspected for ten repeated mensuration and the lowest RSD percentages were observed as about 5 % for the concentration of 1, 10 and 20 nU/mL. As a proof of the discussed results, the presented biosensor has a high sufficiency to be used in clinical laboratories to diagnose pancreatic cancer. We believe that our fabricated sensor can be the plan of diseases management program due to affordable rapid and label free detection of a targeted biomarkers.

{"title":"Highly efficient assessment and selective determination of pancreatic cancer biomarker CA-19-9 using co-electrodeposited MXene-AgNPs nanocomposite","authors":"Mahsa Mirzayi , Balal Khalilzadeh , Sattar Akbari Nakhjavani , Rahim Mohammad-Rezaei","doi":"10.1016/j.sbsr.2025.100753","DOIUrl":"10.1016/j.sbsr.2025.100753","url":null,"abstract":"<div><div>An ultrasensitive and label-free electrochemical immunosensor based on MXene-AgNPs nanocomposite is reported for pancreas cancer diagnostics. Ease of deposition, high speed, thickness of deposition and high efficiency of these biosensors are their special and fundamental advantages.</div><div>For this purpose, a pencil graphite electrode (PGE) was modified by silver and MXene (Ti3C2Tx (to increase efficiency and also the active surface area of PGE. Deposition of AgNPs-MXene nanocomposite then immobilization of streptavidin and biotinylated antibody on the electrode increases the sensitivity, conductivity and stability of the designed immunosensor. The electrochemical measurements in the attendance and absence of AgNPs-MXene can causing an increase in electrical currents. To examine the morphology of the developed immunosensor for the purpose of enhancing its optimal performance, the characterization methods employed include Field Emission Scanning Electron Microscopy (FE-SEM), Fourier-Transform Infrared Spectroscopy (FT-IR), and X-ray Diffraction (XRD). The results of the studies projected antibody (Ab) CA-19-9/AgNPs-MXene electrochemical immunosensor as a potential and affordable alternate of conventional methods like ELISA. The desirable LOD was recorded as 1 nU/mL. The repeatability and reproducibility were successfully inspected for ten repeated mensuration and the lowest RSD percentages were observed as about 5 % for the concentration of 1, 10 and 20 nU/mL. As a proof of the discussed results, the presented biosensor has a high sufficiency to be used in clinical laboratories to diagnose pancreatic cancer. We believe that our fabricated sensor can be the plan of diseases management program due to affordable rapid and label free detection of a targeted biomarkers.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100753"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tunable THz supper absorber based on graphene nano ribbons for bio-sensing applications

IF 5.4 Q1 CHEMISTRY, ANALYTICAL

Sensing and Bio-Sensing Research

Pub Date : 2025-02-01 DOI: 10.1016/j.sbsr.2025.100776

Mohamadreza Vatoor, Seyyed Sajjad Tabatabaee, Pejman Shabani

Leveraging periodic arrays of graphene ribbons and a flexible spacer, a highly adjustable THz wave absorber is discussed in the paper. The structure includes two stacked layers while a thick metallic plate covers the structure from beneath. The exploited periodic arrays of graphene ribbons with four different widths are placed on top of the Kapton spacer. The structure is modeled by passive circuit elements as an impedance while numerical full wave simulation is also performed to verify the validity and accuracy of the impedance matching concept. According to simulation results, an acceptable convergence is obtained between two separate simulations while the circuit model is developed by a MATLAB mfile, and full wave analysis is achieved via CST software. Both simulation paths verify multi-band absorption peaks in the THz spectrum with highly reliable and robust absorption peaks. According to the results, six absorption peaks with higher than 90 % adsorption are achieved. The response sensitivity is investigated versus design parameters that show appropriate robustness against geometrical parameters while the response is able to be fully controlled by changing the graphene patterns' chemical potentials. Such an adjustable wave absorber is in great demand for building larger optical systems including medical and security sensors.

{"title":"Tunable THz supper absorber based on graphene nano ribbons for bio-sensing applications","authors":"Mohamadreza Vatoor, Seyyed Sajjad Tabatabaee, Pejman Shabani","doi":"10.1016/j.sbsr.2025.100776","DOIUrl":"10.1016/j.sbsr.2025.100776","url":null,"abstract":"<div><div>Leveraging periodic arrays of graphene ribbons and a flexible spacer, a highly adjustable THz wave absorber is discussed in the paper. The structure includes two stacked layers while a thick metallic plate covers the structure from beneath. The exploited periodic arrays of graphene ribbons with four different widths are placed on top of the Kapton spacer. The structure is modeled by passive circuit elements as an impedance while numerical full wave simulation is also performed to verify the validity and accuracy of the impedance matching concept. According to simulation results, an acceptable convergence is obtained between two separate simulations while the circuit model is developed by a MATLAB mfile, and full wave analysis is achieved via CST software. Both simulation paths verify multi-band absorption peaks in the THz spectrum with highly reliable and robust absorption peaks. According to the results, six absorption peaks with higher than 90 % adsorption are achieved. The response sensitivity is investigated versus design parameters that show appropriate robustness against geometrical parameters while the response is able to be fully controlled by changing the graphene patterns' chemical potentials. Such an adjustable wave absorber is in great demand for building larger optical systems including medical and security sensors.</div></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":"47 ","pages":"Article 100776"},"PeriodicalIF":5.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143578929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0