Sensors and Actuators B: Chemical最新文献_第10页

Carbon paper stabilized SERS sensor with high excitation laser damage threshold 碳纸稳定高激发激光损伤阈值SERS传感器

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-12-26 DOI: 10.1016/j.snb.2025.139356

Guanwei Tao, Xinping Zhang

Surface-enhanced Raman scattering (SERS) sensors offer high-sensitivity, high-specificity, and high-speed detection of low-concentration molecules with low costs. High stability of the device is a key issue in the design and application of this category of sensors. However, SERS detection with strong and high-contrast Raman signals requires strong laser excitation of the target molecules, which may lead to the damage of the metallic nanostructures on the SERS substrates by photothermal effects, reducing largely the reliability of the detection data. In this study, we present a SERS substrate fabricated by depositing silver nanoparticles (AgNPs) onto the polytetrafluoroethylene (PTFE)-modified carbon paper (CP), which is defined as the CP-PTFE-AgNP sensor. The fabrication parameters were systematically adjusted to optimize the SERS-active nanostructures with significantly improved sensing performance. The fabricated sensor exhibits exceptional photostability, maintaining optimal performance even under high excitation laser intensity. Using R6G as a probe molecule, it demonstrates an ultrahigh enhancement factor of 2.66 × 10¹² and a remarkably low detection limit of 10⁻¹⁷ M. Furthermore, the high-sensitivity detection of ciprofloxacin in water and bilirubin in serum implies promising applications of such a design of SERS devices. These experimental results indicate that this highly stable SERS sensor holds great potential for point-of-care testing applications in fields such as environmental monitoring and biomedical diagnostics.

表面增强拉曼散射（SERS）传感器以低成本提供高灵敏度，高特异性和低浓度分子的高速检测。器件的高稳定性是这类传感器设计和应用的关键问题。然而，使用强、高对比度拉曼信号进行SERS检测需要对目标分子进行强激光激发，这可能导致SERS衬底上的金属纳米结构受到光热效应的破坏，大大降低了检测数据的可靠性。在这项研究中，我们提出了一种将银纳米粒子（AgNPs）沉积在聚四氟乙烯（PTFE）改性碳纸（CP）上的SERS衬底，该衬底被定义为CP-PTFE- agnp传感器。系统地调整了制备参数，优化了sers活性纳米结构，显著提高了传感性能。该传感器具有优异的光稳定性，即使在高激发激光强度下也能保持最佳性能。以R6G为探针分子，具有2.66 × 1012的超高增强因子和10 ~ 17 m的极低检出限。此外，对水中环丙沙星和血清中胆红素的高灵敏度检测表明，该设计的SERS装置具有广阔的应用前景。这些实验结果表明，这种高度稳定的SERS传感器在环境监测和生物医学诊断等领域的即时检测应用中具有巨大的潜力。

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引用次数: 0

Au NPs/CuTCPP(Cu) Schottky junction amplified photocathode aptasensor: Using Staphylococcus aureus as model of target analyte Au NPs/CuTCPP（Cu）肖特基结放大光电阴极感应传感器：以金黄色葡萄球菌为目标分析物模型

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-12-26 DOI: 10.1016/j.snb.2025.139395

Chaoyang Cai , Lilong He , Guanya Ji , Lan Li , Minhua Xiang , Xifeng Yin , Qijian Niu , Qian Liu , Xiuxiu Dong

The photocathode-based photoelectrochemical (PEC) aptasensor has the inherent advantage of high immunity to interference for the detection of target in real samples, however it has low photocurrent response resulting in less good sensitivity. Herein, the synthesized positively charged Au nanoparticles (NPs) were adsorbed onto negatively charged CuTCPP(Cu) nanosheets via electrostatic self-assembly to construct a Schottky junction. The photogenerated electrons of CuTCPP(Cu) are captured and transferred by Au NPs under the influence of the internal electric field of the Schottky junction to inhibit the photogenerated electron-hole pairs recombination of CuTCPP(Cu), which significantly enhance the photocurrent response of the PEC system, effectively improving the sensitivity of the system for detecting Staphylococcus aureus (S. aureus) in real samples. Au NPs can also attach aptamers against S. aureus to electrode surfaces via Au-S bonds, and aptamers can bind to the target which led to the decrease of the photocurrent response through the spatial potential resistance effect. Under the optimum conditions, the linear range of the proposed aptasensor is 1.0∼1.0 × 10⁷ CFU/mL, and the limit of detection is 0.6 CFU/mL. Meanwhile, excellent stability, selectivity and reproducibility and successfully used for the determination of S. aureus in milk was recorded. This work provides a new approach for highly sensitive detection of other kinds of PEC aptasensor.

光电阴极光电电化学（PEC）适体传感器在实际样品中检测目标时具有抗干扰能力强的固有优点，但其光电流响应较低，灵敏度不高。本文将合成的带正电的Au纳米粒子（NPs）通过静电自组装吸附在带负电的cucpp （Cu）纳米片上，构建了肖特基结。在肖特基结内部电场的影响下，cucpp （Cu）的光生电子被Au NPs捕获并转移，抑制cucpp （Cu）的光生电子-空穴对重组，从而显著增强了PEC系统的光电流响应，有效提高了系统检测真实样品中金黄色葡萄球菌（S. aureus）的灵敏度。Au NPs还可以通过Au- s键将抗金黄色葡萄球菌适配体附着在电极表面，适配体与靶标结合，通过空间电位电阻效应导致光电流响应降低。在最佳条件下，该传感器的线性范围为1.0 ~ 1.0 × 107 CFU/mL，检出限为0.6 CFU/mL。同时，该方法具有良好的稳定性、选择性和重复性，可成功用于牛奶中金黄色葡萄球菌的检测。本工作为其他类型PEC配体传感器的高灵敏度检测提供了新的途径。

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引用次数: 0

Precise and highly sensitive detection of single nucleotide variations with a PfAgo and individualized probe combined fluorescent assay PfAgo和个体化探针联合荧光分析的精确和高灵敏度的单核苷酸变异检测

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-12-26 DOI: 10.1016/j.snb.2025.139393

Pei Wang , Yan Lin , Huiling Wu , Chenjie Zhao , Bo Guo , Jian Hong , Wenlong Xia , Yixin Tang , Song Gao

Single nucleotide variations (SNVs) hold significant clinical value in the field of precision medicine. SNV detection is a high-resolution nucleic acid test, and it is always challenging to achieve a good balance between accuracy and convenience in developing SNV detection methods. In this study, a PfAgo and individualized probe combined fluorescent assay (PIPFA) has been established for precise and highly sensitive detection of SNVs. PIPFA combines the programmable nuclease PfAgo and the isothermal RPA technology to build a procedure that is user-friendly and feasible with common lab tools, which is suitable for POCT applications. The L452R/Q/M SNVs in the S gene of SARS-CoV-2 has been used as the model target. PIPFA shows a single-nucleotide resolution that can distinguish multiple variations at a single locus or at adjacent loci, and the sensitivity reaches 10⁰-10¹ copies/μL. The PIPFA procedure is convenient, using two constant temperatures for the reaction without thermocycling, and the results can be visually observed under a blue light source. Importantly, this study suggests a clear rule in PfAgo’s preference on single-nucleotide recognition, that the 2nd base from the 5’-phosphorylated end of the gDNA can be well-distinguished by PfAgo. The PIPFA system is an essential technology accumulation in fighting against future epidemic challenges, and is valuable in extending the accessibility of SNV detection in resource-limited regions.

单核苷酸变异（SNVs）在精准医学领域具有重要的临床价值。SNV检测是一种高分辨率的核酸检测，在发展SNV检测方法时，如何在准确性和便捷性之间取得良好的平衡一直是一个挑战。本研究建立了PfAgo和个体化探针联合荧光法（PIPFA），用于snv的精确、高灵敏度检测。PIPFA结合了可编程核酸酶PfAgo和等温RPA技术，构建了一个用户友好且可行的程序，适用于POCT应用。SARS-CoV-2 S基因中的L452R/Q/M snv被用作模型靶点。PIPFA具有单核苷酸分辨率，可以区分单个位点或相邻位点上的多个变异，灵敏度达到100-101拷贝/μL。PIPFA过程方便，使用两个恒定温度进行反应，不需要热循环，并且在蓝色光源下可以直观地观察结果。重要的是，这项研究表明PfAgo对单核苷酸识别的偏好有一个明确的规律，即gDNA 5 '磷酸化末端的第二个碱基可以被PfAgo很好地区分。PIPFA系统是应对未来流行病挑战的重要技术积累，对于在资源有限的地区扩大SNV检测的可及性具有重要价值。

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引用次数: 0

A suboptimal PAM-driven one-tube CRISPR/Cas12a assay for extraction-free and ultra-rapid detection of African swine fever virus 一种次优的pam驱动的单管CRISPR/Cas12a试验，用于无提取和超快速检测非洲猪瘟病毒

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-12-26 DOI: 10.1016/j.snb.2025.139394

Xiaohui Li , You Nie , Sihan Fei , Yinzhong Wen , Qi Lei , Bo Yang , Ding Zhang , Ruiyan Niu , Zilong Sun

African swine fever (ASF), caused by the African swine fever virus (ASFV), is a highly contagious porcine disease characterized by a near 100 % mortality rate. Given the absence of effective vaccines or therapeutics, current containment strategies critically depend on early detection and prompt culling of infected animals. Here, we establish sCRAM (suboptimal protospacer adjacent motif (PAM)-mediated CRISPR RNA and Cas12a nuclease combined with multienzyme isothermal rapid amplification), a rapid and extraction-free diagnostic platform that detects ASFV within 20 min. sCRAM integrates a rapid nucleic acid release step (40°C, 5 min) with a one-tube reaction combining multienzyme isothermal amplification (MIRA) and a suboptimal PAM-mediated CRISPR/Cas12a system (37°C, 15 min), enabling instrument-free visual readout via UV light or lateral flow strips (LFS). sCRAM demonstrates single-copy detection sensitivity and exhibits no cross-reactivity with five common swine pathogens (PRRSV, PPV, JEV, PCV, and PRV). The evaluation of 111 simulated and clinical samples (including blood, plasma, and swabs) revealed a 100 % concordance with qPCR for UV readout and 98.20 % for LFS readout, with both methods exhibiting 100 % specificity. The simplicity, speed, and field adaptability of sCRAM underscore its potential for decentralized ASFV surveillance in resource-limited settings, offering a powerful tool for outbreak containment.

由非洲猪瘟病毒（ASFV）引起的非洲猪瘟（ASF）是一种高度传染性的猪疾病，其特点是死亡率接近100% %。由于缺乏有效的疫苗或治疗方法，目前的遏制战略严重依赖于早期发现和迅速扑杀受感染的动物。在这里，我们建立了sCRAM（次优原间隔邻近基序（PAM）介导的CRISPR RNA和Cas12a核酸酶结合多酶等温快速扩增），这是一个快速且无提取的诊断平台，可在20 min内检测出ASFV。sCRAM集成了快速核酸释放步骤（40°C, 5 min），单管反应结合多酶等温扩增（MIRA）和次优pam介导的CRISPR/Cas12a系统（37°C, 15 min），通过紫外线或侧流条（LFS）实现无仪器的视觉读取。sCRAM具有单拷贝检测敏感性，与5种常见猪病原体（PRRSV、PPV、JEV、PCV和PRV）无交叉反应性。对111个模拟和临床样本（包括血液、血浆和拭子）的评估显示，紫外读数与qPCR的一致性为100 %，LFS读数为98.20 %，两种方法的特异性均为100% %。sCRAM的简单性、速度和现场适应性突出了其在资源有限的环境中分散监测非洲猪瘟的潜力，为疫情控制提供了强有力的工具。

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引用次数: 0

AIE fluorescent sensors with Zn delivery for bio-model imaging and inflammation relief AIE荧光传感器与锌递送生物模型成像和炎症缓解

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-12-26 DOI: 10.1016/j.snb.2025.139380

Juan Ning , Min Zhao , Lizheng Duan , Yibing Wang , Shi Yan , Yanmei Zhou

Zinc (Zn) is a vital trace element essential for plant and animal development, yet its dysregulation is closely linked to inflammatory and tumor microenvironments. Moderate Zn supplementation has emerged as a promising strategy to promote biological growth and alleviate inflammation. Here, we present TPAPy₃, an aggregation-induced emission (AIE) fluorescent sensor featuring a triphenylamine-thiophene fluorophore integrated with a Zn chelation site via a HClO-responsive schiff base. The chelation of Zn strengthened the intramolecular charge transfer effect and enhanced the sensor’s sensitivity to HClO in inflamed tissues. Upon exposure to HClO, oxidative cleavage of the CN bond triggers fluorescence quenching and Zn release to mitigate inflammation, with the fluorescence quantum yield dropping from 34.51 % to 2.08 %. This dual-function design allowed real-time monitoring of inflammation and targeted Zn delivery for therapeutic intervention. Experiments in mammalian cells and zebrafish demonstrated that TPAPy₃-Zn selectively accumulated and visualized inflammatory sites while alleviating inflammation. Arabidopsis studies further showed that TPAPy₃-Zn promoted plant growth and imaging. Together, these results introduced a new strategy for targeted Zn delivery using fluorescent probes, with potential applications in bioimaging, disease diagnosis, and therapy.

锌（Zn）是植物和动物发育所必需的重要微量元素，其失调与炎症和肿瘤微环境密切相关。适量补充锌已成为促进生物生长和减轻炎症的一种有希望的策略。在这里，我们提出了TPAPy3，一种聚集诱导发射（AIE）荧光传感器，其特征是三苯胺-噻吩荧光团通过hcl响应席夫碱与锌螯合位点集成。锌的螯合增强了分子内电荷转移效应，增强了传感器对炎症组织中HClO的敏感性。当暴露于HClO时，CN键的氧化裂解触发荧光猝灭和Zn释放以减轻炎症，荧光量子产率从34.51 %下降到2.08 %。这种双重功能设计允许实时监测炎症和靶向锌递送治疗干预。在哺乳动物细胞和斑马鱼中进行的实验表明，TPAPy3-Zn在减轻炎症的同时选择性地积聚和显示炎症部位。对拟南芥的进一步研究表明，TPAPy3-Zn促进了植物的生长和成像。总之，这些结果介绍了一种利用荧光探针靶向锌递送的新策略，在生物成像、疾病诊断和治疗方面具有潜在的应用前景。

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引用次数: 0

An acid-activated nanoreactor with self-amplified radical toxicity for augmenting chemodynamic-chemotherapy with NIR imaging guidance 一种具有自放大自由基毒性的酸活化纳米反应器，用于近红外成像指导下的化学动力学化疗

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-12-25 DOI: 10.1016/j.snb.2025.139392

Fanpeng Ran , Menglin Zhang , Qihan Wang , Yutong Hou , Liuyan Yu , TianHao Deng , Wei Huang , Jie Zhao , Xiaoli Zhang , Xiaoxiao Shi

Chemotherapy is a fundamental and effective strategy for clinical cancer therapy and has made significant advancements. However, unfathomed challenges such as low drug delivery efficiency and suboptimal therapeutic potency still remain. Herein, we developed a new acid-responsive metal copper ion-driven nanoreactor (denoted as BTCD) synthesized by supramolecular assembly strategy. In the acidic microenvironment of tumors, the nanoreactor enables controllable release of chemotherapeutic drug doxorubicin. Meanwhile, the chemodynamic effect that divalent copper ion converting reactive oxygen species into more toxic hydroxyl radicals make significant DNA damage and immunogenic cell death (ICD) effects are recorded under cellular level. Furthermore, through near-infrared (NIR) fluorescence imaging guide, the distribution level of nanoreactor was monitored in vivo, and its synergistic chemotherapy effect was verified in a breast cancer tumor mouse model. Overall, this work establishes a promising method for developing intelligent nanomedicine for synergetic chemodynamic-chemotherapy in tumor treatment.

化疗是临床肿瘤治疗的基础和有效策略，并取得了显著进展。然而，诸如低药物递送效率和次优治疗效力等未知的挑战仍然存在。在此，我们开发了一种新的酸反应金属铜离子驱动纳米反应器（简称BTCD），该反应器采用超分子组装策略合成。在肿瘤的酸性微环境中，纳米反应器可以实现化疗药物阿霉素的可控释放。同时，在细胞水平下，二价铜离子将活性氧转化为毒性更强的羟基自由基，产生显著的DNA损伤和免疫原性细胞死亡（ICD）效应。此外，通过近红外（NIR）荧光成像引导，监测纳米反应器在体内的分布水平，并在乳腺癌肿瘤小鼠模型中验证其协同化疗作用。本研究为智能纳米药物协同化疗在肿瘤治疗中的应用开辟了一条新的途径。

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引用次数: 0

Dual-mode biosensor using bifunctional methylene blue for self-validated pathogen detection via inner filter effect and electron transfer 双功能亚甲基蓝双模生物传感器通过内部过滤效应和电子转移进行自验证病原体检测

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-12-25 DOI: 10.1016/j.snb.2025.139388

Yi Wu , Ying Zhang , Wenqing Xia , Meiling Zhou , Xiao Yuan , Jingyu Ge , Longsheng Jin , Qinqiang Zhu , Xiaonan Yang , Xingjian Yu , Xiaolong Gu , Weijie Qu , Meisheng Wu

This study develops an electrochemiluminescence/electrochemical (ECL/EC) dual-mode biosensor for sensitive and self-validated detection of Staphylococcus aureus (S. aureus), a pathogenic bacterium that poses significant environmental contamination risks and public health threats. The biosensor employs Ru(bpy)₃^2 + -incorporated MOF nanoflowers as the ECL emitter and methylene blue (MB) as a dual-functional probe for both electrochemical readout and ECL quencher. Its working principle relies on a combined mechanism involving the inner filter effect (IFE) and electron transfer, which eliminates the strict distance constraints. Upon introduction of the target bacteria (S. aureus), they are specifically captured by the Fe₃O₄/aptamer:ps conjugates and trigger the release of the ps probes, which initiate the rolling circle amplification (RCA). The resulting long DNA product hybridizes with helper strands and then captured by the sensing interface, leading to dense MB assembly. Owing to its high quenching efficiency, the ECL signal is completely suppressed at high concentration of MB. The biosensor enables the simultaneous ECL suppression and DPV enhancement, providing self-validated detection across 10–10⁷ CFU/mL. Statistical analysis showed no significant difference between the dual-signal measurements and standard reference values (p > 0.05), confirming the high accuracy of the biosensor. This work presents a unique approach that integrates efficient bacterial capture, RCA-driven signal amplification, and MB-mediated dual-response signaling for self-validated detection of hazardous pathogens in environmentally relevant matrices.

本研究开发了一种电化学发光/电化学（ECL/EC）双模生物传感器，用于对金黄色葡萄球菌（S. aureus）的敏感和自我验证检测，金黄色葡萄球菌是一种具有重大环境污染风险和公共卫生威胁的致病菌。该生物传感器采用Ru(bpy)32+掺杂的MOF纳米花作为ECL发射器，亚甲基蓝（MB）作为电化学读出和ECL猝灭的双功能探针。它的工作原理依赖于涉及内部过滤效应（IFE）和电子转移的组合机制，从而消除了严格的距离限制。在引入目标细菌（金黄色葡萄球菌）后，它们被Fe3O4/适体：ps偶联物特异性捕获，并触发ps探针的释放，从而启动滚动圈扩增（RCA）。由此产生的长DNA产物与辅助链杂交，然后被传感界面捕获，导致密集的MB组装。由于其高猝灭效率，ECL信号在高浓度MB下被完全抑制。该生物传感器可以同时抑制ECL和增强DPV，提供10至107 CFU/mL的自验证检测。统计分析显示双信号测量值与标准参考值无显著差异（p > 0.05），证实了生物传感器的高精度。这项工作提出了一种独特的方法，集成了有效的细菌捕获，rca驱动的信号放大和mb介导的双反应信号，用于环境相关基质中危险病原体的自我验证检测。

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引用次数: 0

Double-locked ratiometric fluorescent probe for imaging and evaluation of the antioxidant-tyrosinase inhibition pathway 双锁定比例荧光探针对抗氧化剂-酪氨酸酶抑制途径的成像和评价

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-12-25 DOI: 10.1016/j.snb.2025.139391

Da Feng , Li Guo , Xinyuan Han, Yongle Xiao, Fang Yuan, Jianjian Zhang

Melanoma is one of the most aggressive and life-threatening malignancy, characterized by high invasiveness and limited median survival. Despite of the development of numerous diagnostic methods, accurately identifying melanoma remains a major clinical challenge. To minimize interference from external factors and enable in situ melanoma diagnosis, we designed a ratiometric double-locked fluorescent probe named DLN-Pro, specifically targeting melanosomes. DLN-Pro employs naphthimide as its dye and incorporates a morpholine moiety for precise organelle localization. Upon simultaneous recognition of H₂O₂ and TYR, the intramolecular charge transfer (ICT) process is activated, restoring green fluorescence. Moreover, DLN-Pro efficiently accumulates in melanosomes, allowing accurate melanoma identification through fluorescence intensity ratios while reducing false-positive signals. Collectively, DLN-Pro represents a promising tool for investigating H₂O₂ and TYR-related metabolic pathways and holds significant potential for advancing melanoma diagnosis and TYR inhibitor screening.

黑色素瘤是最具侵袭性和威胁生命的恶性肿瘤之一，其特点是高侵袭性和有限的中位生存期。尽管发展了许多诊断方法，但准确识别黑色素瘤仍然是一个主要的临床挑战。为了最大限度地减少外部因素的干扰，实现黑色素瘤的原位诊断，我们设计了一种名为DLN-Pro的比例双锁荧光探针，专门针对黑素小体。DLN-Pro采用萘酰亚胺作为染料，并结合了morpholine部分，用于精确的细胞器定位。当同时识别H2O2和TYR时，激活分子内电荷转移（ICT）过程，恢复绿色荧光。此外，DLN-Pro在黑素小体中有效积累，可以通过荧光强度比准确识别黑色素瘤，同时减少假阳性信号。总之，DLN-Pro代表了一种有前途的工具，用于研究H₂O₂和TYR相关的代谢途径，并具有推进黑色素瘤诊断和TYR抑制剂筛选的重大潜力。

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引用次数: 0

Dynamic visualization of the physiological regulation of H2O2 in plants using NIR fluorescent probe with large Stokes shift 利用大Stokes位移近红外荧光探针动态显示植物体内H2O2的生理调控

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-12-25 DOI: 10.1016/j.snb.2025.139389

Chenglin Su , Yining Sun , Chen Li, Shuang Cao, Xinyue Li, Qijun Sun, Na Niu, Ligang Chen

Hydrogen peroxide (H₂O₂) serves as a crucial signaling molecule and stress indicator in plants. However, due to its high spatiotemporal heterogeneity in vivo, conventional detection methods struggle to achieve accurate, in situ, and high-resolution monitoring. To address this, a near-infrared fluorescent probe (ODCI-PFS) featuring a large Stokes shift (190 nm) was developed to enable highly sensitive and spatiotemporally resolved imaging of H₂O₂ in plant systems. The probe exhibits a linear response within the concentration range of 0.1–100 μM, with a detection limit as low as 0.09 μM. Furthermore, ODCI-PFS demonstrates excellent photostability, low cytotoxicity, and a tissue penetration depth of up to 120 μm, allowing reliable visual tracking of H₂O₂ distribution in various plant species. The probe was successfully applied to monitor H₂O₂ fluctuations during leaf senescence, diurnal light-responsive changes, and tissue-specific accumulation patterns in roots, stems, and leaves. Under cadmium stress, ODCI-PFS revealed concentration-, time-, and species-dependent dynamics of H₂O₂ accumulation in wheat, rice, and maize, providing visual clues to the divergent defense responses across species. This study confirms that ODCI-PFS serves as an effective tool for elucidating H₂O₂-mediated signal transduction and abiotic stress responses in plants, holding significant value for fundamental research.

过氧化氢（H2O2）是植物体内重要的信号分子和胁迫指示因子。然而，由于其在体内的高度时空异质性，传统的检测方法难以实现准确、原位和高分辨率的监测。为了解决这个问题，研究人员开发了一种具有大Stokes位移（190 nm）的近红外荧光探针（ODCI-PFS），以实现植物系统中H2O2的高灵敏度和时空分辨率成像。探针在0.1 ~ 100 μM的浓度范围内呈线性响应，检出限低至0.09 μM。此外，ODCI-PFS具有优异的光稳定性、低细胞毒性和高达120 μm的组织穿透深度，可以可靠地视觉跟踪各种植物物种中的H2O2分布。该探针成功地应用于监测叶片衰老过程中H2O2的波动、昼夜光响应变化以及根、茎和叶的组织特异性积累模式。在镉胁迫下，ODCI-PFS揭示了小麦、水稻和玉米中H2O2积累的浓度、时间和物种依赖动态，为不同物种间不同的防御反应提供了视觉线索。本研究证实ODCI-PFS是阐明植物h2o2介导的信号转导和非生物胁迫反应的有效工具，具有重要的基础研究价值。

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引用次数: 0

From beacon to bonfire: A concentration-dependent fluorescent cascade for amplified reporting of peroxynitrite flux 从信标到篝火：浓度依赖的荧光级联放大报告过氧亚硝酸盐通量

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-12-25 DOI: 10.1016/j.snb.2025.139387

Wenda Zhong , Juan Zheng , Bing Li , Sajid Muhammad , Yang Lu , Xia Yan , Weiliang Kong , Jinhui Wang , Yufen Zhao

Peroxynitrite (ONOO⁻) is a pivotal reactive species involved in numerous pathological processes, yet its real-time tracking remains challenging due to its short half-life and low physiological concentration. Herein, we report a novel dual-wavelength fluorescent probe, CX-B, engineered for concentration-dependent ONOO⁻ sensing. It incorporates a xanthene-derived scaffold featuring two distinct responsive sites: a carbonate-linked benzylboronic acid pinacol ester for nucleophilic attack and an oxidizable CC bond. At lower ONOO⁻ concentrations (0–20 μM), the benzylboronic acid pinacol ester moiety undergoes hydrolysis, releasing the near-infrared (NIR) emissive product CX-OH (λₑₘ = 774/850 nm, LOD = 145 nM) rapidly (k = 1932 M^−1.s⁻¹). At higher ONOO⁻ levels (20–100 μM), oxidative cleavage of the CC bond in CX-OH yields a blue-shifted xanthone derivative (X-OH, λₑₘ = 555 nm), enabling ratiometric fluorescence detection. This cascade activation mechanism facilitates precise, dual-channel quantification of ONOO⁻ with minimal background interference. CX-B exhibits exceptional selectivity, fast response kinetics, and robust performance across biological settings—effectively visualizing dynamic ONOO⁻ fluctuations in models of breast cancer and acute lung injury. This work presents a powerful chemical tool for interrogating ONOO⁻-associated pathophysiology in real time.

过氧亚硝酸盐（ONOO）是参与许多病理过程的关键反应物质，但由于其半衰期短和生理浓度低，其实时追踪仍然具有挑战性。在这里，我们报告了一种新的双波长荧光探针，CX-B，用于浓度依赖性的ONOO毒血症检测。它结合了一个由杂蒽衍生的支架，具有两个不同的反应位点：一个碳酸酯连接的苯硼酸蒎醇酯亲核攻击和一个可氧化的CC键。在较低的ONOO⁻浓度（0-20 μM）下，苯硼酸蒎醇酯部分发生水解，迅速释放出近红外（NIR）发射产物CX-OH (λₑω = 774/850 nm, LOD = 145 nm) （k = 1932 M−1.s−1）。在较高的ONOO毒度（20-100 μM）下，CX-OH中CC键的氧化裂解产生蓝移的山酮衍生物（X-OH, λₑω = 555 nm），使比例荧光检测成为可能。这种级联激活机制有助于在最小的背景干扰下精确地，双通道地量化ONOO⁻。CX-B在生物环境中表现出卓越的选择性，快速的反应动力学和强大的性能-有效地观察乳腺癌和急性肺损伤模型的动态ONOO -波动。这项工作提供了一个强大的化学工具，用于实时询问ONOO毒血症相关的病理生理。

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