Sensors and Actuators B: Chemical最新文献_第10页

In Planta detection of phosphate uptake in chrysanthemum using a miniaturized electrochemical microprobe array 应用微型电化学探针阵列检测菊花对磷酸盐的吸收

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-23 DOI: 10.1016/j.snb.2026.139541

Zhe Yu , Rui Tang , Minyu Wu , Wenke Zou , Muxuan Chang , Hongqin Mao , Jinyuan Liang , Xiaohui Xu , Cheng Zhang , Fadi Chen

Phosphorus is an essential macronutrient governing key physiological traits in chrysanthemum. However, phosphate nutrition is typically evaluated based on fertilizer input rather than direct in planta uptake, limiting management accuracy. Here, we develop a miniaturized electrochemical microprobe array for direct in planta detection of phosphate in chrysanthemum stem sap. The array integrates a cobalt-based working electrode fabricated via an electroplating-then-etching technique and a titanium-based iridium dioxide reference electrode, all optimized for miniaturization and biocompatibility. The array demonstrated a wide linear detection range (10⁻⁵ to 10⁻¹mol/L), good sensitivity (41 mV/dec), and strong anti-interference performance in the presence of coexisting ions. Biocompatibility tests confirmed minimal disruption to plant growth after short- and long-term probe implantation. In planta measurements revealed spatial variation in phosphate distribution along the stem and dynamic uptake responses following fertilizer application. These findings highlight the potential of this microprobe array for precise nutrient uptake analysis and sustainable phosphorus management in horticultural systems.

磷是控制菊花关键生理性状的必需常量营养素。然而，磷酸盐营养通常是根据肥料投入而不是直接根据植物吸收来评估的，这限制了管理的准确性。在这里，我们开发了一种小型化的电化学微探针阵列，用于直接在植物中检测菊花茎液中的磷酸盐。该阵列集成了一个通过电镀-蚀刻技术制造的钴基工作电极和一个钛基二氧化铱参考电极，所有这些都经过了小型化和生物相容性优化。该阵列具有宽的线性检测范围（10−5 ~ 10−1mol/L），良好的灵敏度（41 mV/dec），在共存离子存在下具有较强的抗干扰性能。生物相容性试验证实，在短期和长期探针植入后，对植物生长的破坏最小。在植物测量中揭示了磷素沿茎的分布和施肥后的动态吸收响应的空间变化。这些发现突出了该微探针阵列在园艺系统中精确养分吸收分析和可持续磷管理方面的潜力。

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引用次数: 0

Novel G-quadruplex probe coupled with CRISPR/Cas12a for ultrasensitive and highly specific nucleic acid testing 新型g -四重体探针与CRISPR/Cas12a偶联，用于超灵敏和高特异性核酸检测

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-23 DOI: 10.1016/j.snb.2026.139508

Huan Jiang , Wenwen Wu , Shiyue Lu , Yuan Xu , Hong Zhang , Shu Shang , Xin Yang , Hongfei He , Qiang Wang , Hanfeng Yang , Guangcheng Luo

Integrating CRISPR/Cas12a with cost-effective probes is crucial for molecular diagnostics. However, a fluorophore-quencher-labeled ssDNA probe, a classic tool in this field, entails high production costs. Herein, we developed a novel quencher-free fluorescent G-quadruplex (G4) probe with excellent cost-effectiveness for CRISPR/Cas12a. We found that FAM fluorescein could be efficiently quenched by G4, while the incorporation of 3′-flanking sequences into the single FAM-labeled G4 could destabilize its topological structure. This enabled efficient trans-cleavage by CRISPR/Cas12a, thereby releasing the G4-quenched fluorescence. Compared with conventional turn-off CRISPR/Cas12a/G4 systems, this G4-assisted CRISPR/Cas12a Reporter (GCR) innovatively achieves cost-effective turn-on signal output. Further, we integrated GCR with previously developed dNTPαS-assisted RPA to establish an RPA/GCR assay. This assay achieved ultra-sensitivity (up to 16 aM) and single-base discrimination ability, enabling HBV DNA detection with 100 % concordance with the qPCR results. In summary, we developed a novel strategy for turn-on CRISPR/Cas12a/G4 signal output and established a practical RPA/GCR assay, highlighting their significant potential for molecular diagnostics.

将CRISPR/Cas12a与具有成本效益的探针相结合对于分子诊断至关重要。然而，荧光团猝灭剂标记的ssDNA探针是该领域的经典工具，其生产成本很高。在此，我们开发了一种新型的无猝灭剂荧光g -四重体（G4）探针，具有优异的成本效益。我们发现FAM荧光素可以被G4有效猝灭，而在单个FAM标记的G4中加入3 ' -侧翼序列会破坏其拓扑结构的稳定性。这使得CRISPR/Cas12a能够高效地进行反式切割，从而释放g4猝灭的荧光。与传统的关断CRISPR/Cas12a/G4系统相比，G4辅助CRISPR/Cas12a Reporter （GCR）创新性地实现了低成本的开断信号输出。此外，我们将GCR与先前开发的dntp α s辅助RPA结合，建立了RPA/GCR检测。该试验具有超灵敏度（高达16 aM）和单碱基识别能力，使HBV DNA检测与qPCR结果具有100% %的一致性。总之，我们开发了一种开启CRISPR/Cas12a/G4信号输出的新策略，并建立了一种实用的RPA/GCR检测方法，突出了它们在分子诊断方面的巨大潜力。

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引用次数: 0

Highly sensitive detection of epithelial markers on single tumor cell surface by an endonuclease-assisted assay on superwettable droplet microarrays (EASDM) 内切酶辅助超湿微滴阵列（EASDM）检测单个肿瘤细胞表面上皮标记物的研究

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-23 DOI: 10.1016/j.snb.2026.139524

Xinxing Miao , Shanzhou Duan , Xiang Xiao , Sidi Liu , Qinghua Cao , Chengcheng Tao , Jian Zhao , Mengdie Zhou , Yongbing Chen , Anna Zaczek , Jian Liu

Epithelial cell adhesion molecule (EpCAM) serves a key biomarker for epithelial cancers detection in clinical diagnostics. There is a great need to develop highly sensitive methods for EpCAM-positive cell detection with a high throughput performance. Here we report an aptamer-mediated signal amplification strategy, that is, Endonuclease-Assisted Assay on Superwettable Droplet Microarrays (EASDM), for ultrasensitive EpCAM detection on single tumor cell surface. We have demonstrated that EASDM achieves sensitive detection of EpCAM on live tumor cell surface, with a limit of detection (LOD) as low as a single cell. It has been validated to distinguish MCF-7 cells (high EpCAM expression), MDA-MB-231 cells (low EpCAM expression), and white blood cells (negative control of EpCAM expression), or the tumor cells under chemically-induced epithelial-mesenchymal transition. We have further verified the robust performance of EASDM by using human whole blood samples spiked with cancer cells. This pilot platform integrating aptamer-based enzymatic amplification and superwettable droplet microarrays promises advancing cancer diagnostics/prognostics with high throughput, sensitivity, and accuracy for personalized medicine.

上皮细胞粘附分子（Epithelial cell adhesion molecule, EpCAM）是临床诊断上皮性癌症的关键生物标志物。目前迫切需要开发高灵敏度、高通量的epcam阳性细胞检测方法。在这里，我们报告了一种适体介导的信号扩增策略，即内切酶辅助超湿滴微阵列（EASDM）检测，用于在单个肿瘤细胞表面进行超灵敏的EpCAM检测。我们已经证明EASDM在活肿瘤细胞表面实现了EpCAM的灵敏检测，检测限低至单个细胞。已证实可区分MCF-7细胞（EpCAM高表达）、MDA-MB-231细胞（EpCAM低表达）和白细胞（EpCAM阴性表达），或化学诱导的上皮-间质转化的肿瘤细胞。我们通过使用含有癌细胞的人全血样本进一步验证了EASDM的稳健性能。这个试点平台集成了基于适配体的酶扩增和超湿滴微阵列，有望以高通量、灵敏度和准确性推进癌症诊断/预后，用于个性化医疗。

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引用次数: 0

A colorimetric-photothermal-fluorescent triple-mode nanozyme sensor array: Toward machine learning driven detection and recognition of β-lactam antibiotics 比色-光热-荧光三模纳米酶传感器阵列：面向机器学习驱动的β-内酰胺类抗生素检测和识别

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-22 DOI: 10.1016/j.snb.2026.139536

Yu Wang, Zemin Ren, Wen Zhang, Fufeng Liu, Wenjie Jing

β-lactam antibiotics (β-LAs) are widely used as anti-infective drugs, but their residues exert a serious risk to public health and the environment. Consequently, the development of simple and efficient methods for β-LAs detection is particularly important. Here, we found that β-LAs notably hinder the peroxidase-like (POD) activity of copper hydroxide nitrate (Cu₂(OH)₃NO₃) nanozyme. Based on the unique physicochemical properties of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB), a colorimetric-photothermal-fluorescent triple-mode nanozyme array sensor was constructed and successfully used for the efficient detection and differentiation of eight β-LAs. Moreover, through the optimization of several machine learning (ML) algorithms, the accuracy of the concentration-independent classification model built on this array was enhanced from 57.29 % to 90.62 %, facilitating the recognition of blind samples. More notably, integrating sensor arrays with regression algorithms allows for accurate quantitative determination of various β-LAs. The research holds considerable importance for enhancing β-LAs recognition in complex matrices.

β-内酰胺类抗生素被广泛用作抗感染药物，但其残留对公众健康和环境造成严重威胁。因此，开发简单有效的β-LAs检测方法尤为重要。本研究发现，β-LAs明显抑制了氧化铜硝酸（Cu2(OH)3NO3）纳米酶的过氧化物酶样（POD）活性。基于显色底物3,3 ',5,5 ' -四甲基联苯胺（TMB）独特的理化性质，构建了比色-光热-荧光三模式纳米酶阵列传感器，并成功用于8种β-LAs的高效检测和鉴别。此外，通过对几种机器学习算法的优化，在该阵列上建立的浓度无关分类模型的准确率从57.29 %提高到90.62 %，有利于盲样本的识别。更值得注意的是，将传感器阵列与回归算法相结合，可以准确定量测定各种β-LAs。该研究对增强复杂矩阵中β-LAs的识别具有重要意义。

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引用次数: 0

Reusable RF sensor based on solution-processed MoS2 for liquid biopsy of single-stranded circulating cell-free DNA 基于溶液处理MoS2的可重复使用射频传感器用于单链循环无细胞DNA的液体活检

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-22 DOI: 10.1016/j.snb.2026.139540

Seungchan Lee , Eunho Choi , Jieun Kim , Sungmoon Park , Changwoo Pyo , Dohyun Lee , Joohoon Kang , Woojung Shin , Myungsoo Kim

Reliable and cost-effective detection of single-stranded circulating cell-free DNA (ss-cfDNA) is crucial for liquid biopsies, offering a non-invasive method for precise cancer diagnosis and therapeutic monitoring. This work reports a reusable, label-free radio frequency (RF) biosensor utilizing solution-processed molybdenum disulfide (MoS₂) thin films on an interdigitated capacitor. The spin-coating fabrication of the MoS₂ layer offers superior scalability and cost-effectiveness compared to traditional 2D material growth and transfer. The sensing mechanism is initiated by the adsorption of ss-cfDNA nucleobases onto the MoS₂ basal plane via van der Waals forces. This interaction concurrently induces n-type doping and alters the local dielectric environment, causing detectable impedance alterations manifested as resonant frequency shifts and time-domain reflection changes. The sensor achieves a limit of detection of 154.67 nM for a 20-base ss-cfDNA sequence from AluSx1, a key ALU element in cancer diagnostics, demonstrating high specificity for ssDNA over double-stranded DNA. Detecting ss-cfDNA of various lengths further validates the mechanism and establishes broad applicability. Moreover, the sensor demonstrates robust reusability (≥ 5 cycles) and 7-day stability using a complementary DNA-mediated regeneration process, which is comprehensively validated by a suite of analytical techniques. This solution-processable, label-free RF biosensor is a promising platform for cost-effective and scalable ss-cfDNA detection, distinguished by its simple fabrication, specificity, and reusability.

可靠且具有成本效益的单链循环无细胞DNA （ss-cfDNA）检测对于液体活检至关重要，为精确的癌症诊断和治疗监测提供了一种非侵入性方法。这项工作报告了一种可重复使用的，无标签的射频（RF）生物传感器，利用溶液处理的二硫化钼（MoS2）薄膜在交叉电容上。与传统的二维材料生长和转移相比，MoS2层的自旋涂层制造具有优越的可扩展性和成本效益。该传感机制是由ss-cfDNA核碱基通过范德华力吸附在MoS2基面上引起的。这种相互作用同时诱导n型掺杂并改变局部介电环境，引起可检测的阻抗变化，表现为谐振频移和时域反射变化。该传感器对来自AluSx1的20个碱基的ss-cfDNA序列（癌症诊断中的关键ALU元件）的检测限为154.67 nM，表明ssDNA比双链DNA具有高特异性。检测不同长度的ss-cfDNA进一步验证了该机制并建立了广泛的适用性。此外，该传感器具有强大的可重复使用性（≥5个循环）和7天的稳定性，使用互补的dna介导的再生过程，这是通过一套分析技术全面验证的。该解决方案可处理，无标签射频生物传感器是一个有前途的平台，具有成本效益和可扩展的ss-cfDNA检测，其特点是其简单的制造，特异性和可重用性。

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引用次数: 0

Ammonia-etched hollow Prussian blue@Au nanoframes performing ultrasensitive pyruvate monitoring in fermentation 氨蚀刻中空普鲁士blue@Au纳米框架在发酵中进行超灵敏丙酮酸监测

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-22 DOI: 10.1016/j.snb.2026.139538

Yixue Chen , Huaiyu Song , Yan Qin , Yu Liu , Ying Xie , Zhenyu Chu , Wanqin Jin

Pyruvate is one of the most important intermediate metabolites in the fermentation process, thereby making it a potential indicator for monitoring and regulating the fermentation process. Since the fermentation broth is usually complex, including microorganisms, various ions, organic compounds, etc., it is necessary to develop a highly selective method for detecting pyruvate. Therefore, in this work, we proposed an ultrasensitive electrochemical enzymatic biosensor for pyruvate determination at a low working potential through constructing Prussian blue@Au nanoframes (PB@Au NFs) with improved conductivity and high electrocatalytic ability. Due to the strong catalytic ability of PB nanoframes for the reduction of H₂O₂, the as-prepared sensor initially demonstrated extremely 2.7-fold sensitivity toward H₂O₂ than the non-frame structured PB nanocubes. Furthermore, after the conditions for pyruvate detection were systematically optimized, the fabricated sensor was able to effectively identify pyruvate ranging from 5 μM to 0.895 mM at −0.2 V which represented an orders-of-magnitude improvement in sensitivity over existing sensors. Meanwhile, the low working potential and the highly precise recognition ability of pyruvate oxidase ensured the high selectivity of the prepared sensor, and the sensor also showed excellent reproducibility and long-term stability. Finally, our biosensor was employed to detect pyruvate in the real microbial fermentation liquid, giving an excellent accuracy with RSD values of 1.44 % - 4.64 %. This work provides a new strategy approach for the design and establishment of stable pyruvate sensors and offered a usefulness approach for the pyruvate detection with high performance.

丙酮酸是发酵过程中最重要的中间代谢物之一，是监测和调控发酵过程的潜在指标。由于发酵液通常是复杂的，包括微生物、各种离子、有机化合物等，因此有必要开发一种高选择性的丙酮酸盐检测方法。因此，在这项工作中，我们提出了一个超灵敏的电化学酶生物传感器丙酮酸测定在低工作电位通过构建普鲁士blue@Au纳米框架（PB@Au NFs）具有提高电导率和高电催化能力。由于PB纳米框架对H2O2还原的催化能力较强，所制备的传感器对H2O2的灵敏度是非框架结构PB纳米立方体的2.7倍。此外，在系统优化丙酮酸检测条件后，该传感器能够在−0.2 V下有效识别5 μM至0.895 mM范围内的丙酮酸，灵敏度比现有传感器提高了几个数量级。同时，丙酮酸氧化酶的低工作电位和高精度识别能力保证了所制备传感器的高选择性，并表现出良好的重现性和长期稳定性。最后，将该传感器用于实际微生物发酵液中丙酮酸的检测，RSD值为1.44 % ~ 4.64 %，准确度较高。本研究为设计和建立稳定的丙酮酸盐传感器提供了新的策略途径，为丙酮酸盐的高效检测提供了有用的方法。

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引用次数: 0

Dual-mode sensor based on Co-doped Zn-based phosphide and triple multivalent aptamers within tetrahedron DNA for clothianidin detection 基于共掺杂锌基磷化物和四面体DNA内三重多价适体的双模传感器用于衣虫胺检测

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-22 DOI: 10.1016/j.snb.2026.139522

Peiyuan Wei , Wei Han , Ting Cheng , Lingling Xie , Hongjun Chen , Yuling Wang , Lei Wang , Limin Zhu , Baoshan He , Xiaoyu Cao

This paper aims to effectively detect clothianidin (CLD) residues, a neonicotinoid insecticide, to prevent food safety issues and mitigate the impact of environmental change. A novel Co-doped Zn-based phosphide hollow microsphere (CoZnP@C) was synthesized through a hydrothermal method followed by calcination. The rich carboxyl group, metal-C, metal-O, or metal-P chelates of CoZnP@C result in high peroxidase-like catalytic and electrochemical activity. Based on this, we creatively combined CoZnP@C with magnetic beads directly and then absorbed complementary DNA as a dual-mode signal probe. When CLD is present, CLD competitively binds with the designed tetrahedral DNA nanostructure (TDN) incorporating triple multivalent aptamers (t-TDN), resulting in the probe being released for colorimetric and electrochemical detection. Under optimal conditions, the dual-mode sensor exhibits a low detection limit of 2.37 pM in colorimetric mode and 68.88 pM in electrochemical mode, along with a wide linear range of 0.01 nM – 100 μM. The developed sensor is characterized by high selectivity, reproducibility, stability, and rapid response in colorimetric mode, with a response time of 6 min. It is exploited to monitor the residue of CLD in tap water and apples, offering a promising and wide applications in food quality control and environmental assessment.

本文旨在有效检测新烟碱类杀虫剂噻虫胺（clothianidin， CLD）残留，以预防食品安全问题，减轻环境变化的影响。采用水热煅烧法制备了一种新型共掺杂锌基磷化物空心微球（CoZnP@C）。CoZnP@C丰富的羧基、金属- c、金属- o或金属- p螯合物具有高的过氧化物酶样催化和电化学活性。在此基础上，我们创造性地将CoZnP@C与磁珠直接结合，然后吸收互补DNA作为双模信号探针。当CLD存在时，CLD与设计的包含三重多价适体（t-TDN）的四面体DNA纳米结构（TDN）竞争性结合，导致探针被释放用于比色和电化学检测。在最佳条件下，双模传感器的检测限在比色模式下为2.37 pM，在电化学模式下为68.88 pM，线性范围为0.01 nM - 100 μM。该传感器在比色模式下具有高选择性、重复性、稳定性和快速响应的特点，响应时间为6 min。该方法可用于自来水和苹果中CLD的残留监测，在食品质量控制和环境评价方面具有广阔的应用前景。

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引用次数: 0

Dopant-induced carrier mobility refinement in g-C3N4 and SnO2 composites for enhanced room temperature NO2 sensing 掺杂剂诱导的g-C3N4和SnO2复合材料载流子迁移率细化增强了室温NO2传感

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-22 DOI: 10.1016/j.snb.2026.139468

Xiao Wang , Yuli Zhao , Mingyuan Wang , Zhaokun Sun , Xinyu Li , Ce Guo

The incorporation of non-metal elements into the g-C₃N₄ framework introduces localized lattice distortion and electronic reorganization, which collectively modulate the carrier concentration and bandgap alignment, ultimately enhancing charge transfer and gas adsorption abilities. Herein, B, S, Cl and P elements were successfully doped into g-C₃N₄ to optimize the localized electronic structure and charge carrier density. After that, SnO₂ composites were decorated on the modified g-C₃N₄ support for gas sensing applications. A significant electron transfer from B doped g-C₃N₄ (B-CN) to SnO₂ can be confirmed, generating a thick electron accumulation layer. Consequently, more NO₂ gas molecules will be adsorbed on the sensing materials. Moreover, Bader charge analyses indicate that the SnO₂/B-CN composite exhibits significantly enhanced charge transfer (0.68 e) compared to SnO₂/g-C₃N₄ (0.22 e) upon NO₂ exposure, leading to enhanced sensing response. Gas sensing results show that the response of SnO₂/B-CN sensors to 10 ppm NO₂ reaches 247 at room temperature (RT, 25°C). Moreover, a high selectivity, fast sensing transient (6 s and 11 s) and low detection limit of 56 ppb at RT can be also reached. Finally, an intelligent wireless detection platform was developed incorporating a gas sensor, microcontroller and Wi-Fi communication module for real-time cloud-based data transmission and remote gas monitoring. Crucially, this work establishes a comprehensive mechanistic framework for non-metal dopant-induced electronic structure modulation in g-C₃N₄, while proposing an innovative protocol to optimize its gas-sensing functionality.

非金属元素加入到g-C3N4框架中引入了局域晶格畸变和电子重组，它们共同调节载流子浓度和带隙排列，最终增强电荷转移和气体吸附能力。本文成功地将B、S、Cl和P元素掺杂到g-C3N4中，优化了局域电子结构和载流子密度。然后，在改性的g-C3N4支架上装饰SnO2复合材料，用于气敏应用。可以证实，掺杂B的g-C3N4 （B- cn）向SnO2发生了明显的电子转移，形成了厚的电子积累层。因此，更多的NO2气体分子将被吸附在传感材料上。此外，Bader电荷分析表明，SnO2/B-CN复合材料在NO2暴露下的电荷转移（0.68 e）比SnO2/g-C3N4 （0.22 e）显著增强，从而增强了传感响应。气体传感结果表明，SnO2/B-CN传感器在室温（RT, 25℃）下对10 ppm NO2的响应达到247。此外，该方法还具有高选择性、快速检测瞬态（6 s和11 s）和低检出限（56 ppb）。最后，结合气体传感器、微控制器和Wi-Fi通信模块，开发了智能无线检测平台，实现基于云的实时数据传输和远程气体监测。至关重要的是，这项工作为g-C3N4中非金属掺杂诱导的电子结构调制建立了一个全面的机制框架，同时提出了一个创新的协议来优化其气敏功能。

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引用次数: 0

Programmable dual-fluorescence DNA aptasensor using cascade amplification and sequence-engineered AgNCs for ultrasensitive detection of Clostridioides difficile RNase H2 使用级联扩增和序列工程agnc的可编程双荧光DNA适体传感器用于艰难梭菌RNase H2的超灵敏检测

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-21 DOI: 10.1016/j.snb.2026.139533

Hu Zhang , Ying Zhao , Hui Meng , Xing Dong , Zhen Zhang , Jianxia Lv , Hongjun Zhao

Rapid and accurate detection of Clostridioides difficile remains challenging due to the limited sensitivity, complex protocols, and costly instrumentation required by current diagnostic assays. Here, we present a label-free, enzyme-free dual-fluorescence aptasensor that integrates entropy-driven catalysis (EDC) and catalytic hairpin assembly (CHA) with DNA-templated silver nanoclusters (DNA-AgNCs) for the sensitive and reliable detection of RNase H2, a highly specific biomarker of C. difficile. Aptamers immobilized on magnetic beads selectively bind RNase H2 and release complementary primers that trigger a cascaded EDC-CHA amplification network. The amplified primers modulate the conformation of AgNC-templating hairpins, enabling sequence-directed tuning of AgNC emission. By tuning the nucleation sequence, Two AgNC emitters respond inversely: red fluorescence is enhanced while yellow emission is simultaneously quenched. This ratiometric dual-signal mechanism provides intrinsic self-correction against environmental fluctuations and significantly improves quantitative accuracy. Under optimized conditions, the sensor exhibits a broad linear detection range (0.01–100 ng/mL) and an ultralow detection limit of 9.27 pg/mL, along with high specificity and accurate recovery in river water samples. This sequence-programmable DNA–AgNC platform establishes a robust, cost-effective strategy for rapid pathogen diagnostics.

由于目前的诊断检测方法灵敏度有限、方案复杂、仪器昂贵，快速准确地检测艰难梭菌仍然具有挑战性。在这里，我们提出了一种无标记、无酶的双荧光适配体传感器，它将熵驱动催化（EDC）和催化发夹组装（CHA）与dna模板化银纳米团簇（dna - agnc）结合在一起，用于敏感可靠地检测艰难梭菌高度特异性的生物标志物RNase H2。固定在磁珠上的适配体选择性地结合RNase H2并释放互补引物，从而触发级联的EDC-CHA扩增网络。扩增的引物可调节AgNC模板发卡的构象，从而实现AgNC发射的序列定向调节。通过调整成核序列，两个AgNC发射体的响应相反：红色荧光增强，而黄色荧光同时被淬灭。这种比率双信号机制对环境波动提供了内在的自我校正，并显著提高了定量准确性。在优化条件下，该传感器具有较宽的线性检测范围（0.01 ~ 100 ng/mL）和9.27 pg/mL的超低检出限，在河流水样中具有较高的特异性和准确的回收率。这个序列可编程DNA-AgNC平台为快速病原体诊断建立了一个强大的、具有成本效益的策略。

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引用次数: 0

Endogenous fluorescent metal-organic framework ratio fluorescence sensing strategy: Combining CRISPR/Cas12a for ultra-sensitive detection of tetracycline 内源性荧光金属-有机框架比荧光传感策略：结合CRISPR/Cas12a超灵敏检测四环素

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-21 DOI: 10.1016/j.snb.2026.139539

Huikai Lin , Caiyi Du , Ningyi Xu , Yiru Wang , Chunyan Sun

CRISPR/Cas12a can activate trans-cleavage activity upon single-stranded DNA binding, making it widely applicable in highly sensitive fluorescent biosensors. Sensors utilizing monochromatic fluorescence output modes are susceptible to interference from multiple factors, resulting in unstable detection methods. To address this, we designed a ratio-based fluorescence sensor for detecting tetracyclines (TCs) based on the synergistic signal amplification of nucleic acid aptamer (42TET) and zirconium-based metal-organic framework (PCN-224) with CRISPR/Cas12a. PCN-224 adsorbs single-stranded DNA, quenching its FAM-labeled fluorescence while simultaneously emitting its own fluorescent signal. This dual-fluorescence output reduces background interference and enhances detection accuracy. By designing an activator as a complementary sequence (cDNA) to the nucleic acid aptamer, the system binds to 42TET in the presence of tetracycline, releasing the cDNA to activate the CRISPR/Cas12a system. Through this system's signal amplification capability, highly sensitive detection of tetracycline is achieved, with a detection limit reaching 8.7 nM. It possesses the same detection capabilities as traditional HPLC detection methods. This method is simple, rapid, efficient, and highly quantitative, offering broad prospects for future applications.

CRISPR/Cas12a可以在单链DNA结合时激活反式裂解活性，广泛应用于高灵敏度的荧光生物传感器。利用单色荧光输出模式的传感器容易受到多种因素的干扰，导致检测方法不稳定。为了解决这一问题，我们设计了一种基于核酸适配体（42TET）和锆基金属有机框架（PCN-224）与CRISPR/Cas12a协同信号扩增的比例荧光传感器，用于检测四环素（TCs）。PCN-224吸附单链DNA，猝灭其fam标记的荧光，同时发出自己的荧光信号。这种双荧光输出减少了背景干扰，提高了检测精度。通过将激活子设计为核酸适体的互补序列（cDNA），该系统在四环素存在下与42TET结合，释放cDNA激活CRISPR/Cas12a系统。通过该系统的信号放大能力，实现了对四环素的高灵敏度检测，检测限达到8.7 nM。它具有与传统HPLC检测方法相同的检测能力。该方法简便、快速、高效、定量强，具有广阔的应用前景。

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