Pub Date : 2025-01-31DOI: 10.1016/j.snb.2025.137370
Zi Yi Xu , Jia Shan Xia , Rong Wang , Qi Xiao , Hong Qun Luo , Tao Deng , Nian Bing Li
Fluorescence imaging has provided abundant information in biological researches, which unveils the microscopic nexuses in physiological and pathological processes. Nevertheless, this main-stream solution is always impeded by intractable drawbacks of imaging tools including small Stokes shift, short-wavelength emission, inferior photostability, and insufficient fluorescence quantum yield (ΦF). In this contribution, an ingenious molecular platform constituted of three novel dicyanocoumarin analogues is rationally engineered. With large Stokes shift, its fluorescence is located in the far-red region. Serendipitously, an unexpected phenomenon is uncovered that emission of these novel compounds enhances along with the increase of polarity, which confers them considerable ΦF. Due to such merits, this multifunctional fluorescence platform is applied to cellular imaging due to its multivariate spectral responsiveness toward pH, polarity, and hypochlorous acid (HClO). Besides, in vivo imaging of ferroptosis and wound inflammation mouse models has also revealed the great potential of this fluorescence platform. Performing eminent bioimaging results, we conceive that this fluorescence platform would be a robust imaging toolbox in the future, which provides designing inspiration for development of high-performance fluorophores.
{"title":"Harnessing a multifunctional far-red fluorescence platform for high-performance biological sensing and imaging","authors":"Zi Yi Xu , Jia Shan Xia , Rong Wang , Qi Xiao , Hong Qun Luo , Tao Deng , Nian Bing Li","doi":"10.1016/j.snb.2025.137370","DOIUrl":"10.1016/j.snb.2025.137370","url":null,"abstract":"<div><div>Fluorescence imaging has provided abundant information in biological researches, which unveils the microscopic nexuses in physiological and pathological processes. Nevertheless, this main-stream solution is always impeded by intractable drawbacks of imaging tools including small Stokes shift, short-wavelength emission, inferior photostability, and insufficient fluorescence quantum yield (<em>Φ</em><sub>F</sub>). In this contribution, an ingenious molecular platform constituted of three novel dicyanocoumarin analogues is rationally engineered. With large Stokes shift, its fluorescence is located in the far-red region. Serendipitously, an unexpected phenomenon is uncovered that emission of these novel compounds enhances along with the increase of polarity, which confers them considerable <em>Φ</em><sub>F</sub>. Due to such merits, this multifunctional fluorescence platform is applied to cellular imaging due to its multivariate spectral responsiveness toward pH, polarity, and hypochlorous acid (HClO). Besides, in vivo imaging of ferroptosis and wound inflammation mouse models has also revealed the great potential of this fluorescence platform. Performing eminent bioimaging results, we conceive that this fluorescence platform would be a robust imaging toolbox in the future, which provides designing inspiration for development of high-performance fluorophores.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137370"},"PeriodicalIF":8.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-31DOI: 10.1016/j.snb.2024.137147
Laura Belcastro , Paolo Antonacci , Gaia Rocchitta , Christoph M. Sprecher , Andrea J. Vernengo , Pier Andrea Serra , Fabiana Arduini , Sibylle Grad , Valentina Basoli
Nitric oxide (NO), a key metabolite released during inflammation, can be a reliable biomarker for conditions such as trauma, infection or during inflammation. Standard NO detection methods, including electron paramagnetic resonance (EPR) and indirect detection of nitrites as NO derivatives, using Griess assay, are limited in their ability to provide real-time monitoring, a critical feature for studying drug responses and enabling clinical applications. Electrochemical sensors can be used for continuous real-time NO measurement but require careful design to ensure selectivity in complex biological environments. In this study, platinum wire electrodes were used, either unmodified or coated with poly-o-phenylenediamine (PPD) polymer, to improve selectivity. These sensors were calibrated using S-nitroso-N-acetylpenicillamine (SNAP) molecule, an NO-donor, and tested against common biological interferents, such as ascorbic acid, fetal bovine serum, antibiotics, and several electrolytes. For real time NO detection, a tissue culture plate was customized to allow sensor connection guaranteeing a closed and sterile environment for cell culture. Amperometry was performed over 48 h in 2D chondrocyte inflammation model cultures with and without IL-1β stimulation. Results demonstrated that the PPD coating was homogeneously distributed on the sensor surface and significantly reduced background noise from ascorbic acid. Furthermore, PPD-modified sensors showed enhanced selectivity and reproducibility during real-time NO monitoring, particularly in distinguishing NO levels in inflamed versus non-inflamed chondrocyte cultures. The findings highlight the importance of surface modifications to mitigate interference from non-relevant analytes in complex biological environments. These results suggest that real-time NO monitoring with electrochemical sensors could offer a valuable tool for assessing inflammation, with potential future applications in both in vitro and in vivo settings.
{"title":"Real-time nitric oxide and inflammation sensing in 2D osteoarthritis models: microsensor design and application","authors":"Laura Belcastro , Paolo Antonacci , Gaia Rocchitta , Christoph M. Sprecher , Andrea J. Vernengo , Pier Andrea Serra , Fabiana Arduini , Sibylle Grad , Valentina Basoli","doi":"10.1016/j.snb.2024.137147","DOIUrl":"10.1016/j.snb.2024.137147","url":null,"abstract":"<div><div>Nitric oxide (NO), a key metabolite released during inflammation, can be a reliable biomarker for conditions such as trauma, infection or during inflammation. Standard NO detection methods, including electron paramagnetic resonance (EPR) and indirect detection of nitrites as NO derivatives, using Griess assay, are limited in their ability to provide real-time monitoring, a critical feature for studying drug responses and enabling clinical applications. Electrochemical sensors can be used for continuous real-time NO measurement but require careful design to ensure selectivity in complex biological environments. In this study, platinum wire electrodes were used, either unmodified or coated with poly-o-phenylenediamine (PPD) polymer, to improve selectivity. These sensors were calibrated using S-nitroso-N-acetylpenicillamine (SNAP) molecule, an NO-donor, and tested against common biological interferents, such as ascorbic acid, fetal bovine serum, antibiotics, and several electrolytes. For real time NO detection, a tissue culture plate was customized to allow sensor connection guaranteeing a closed and sterile environment for cell culture. Amperometry was performed over 48 h in 2D chondrocyte inflammation model cultures with and without IL-1β stimulation. Results demonstrated that the PPD coating was homogeneously distributed on the sensor surface and significantly reduced background noise from ascorbic acid. Furthermore, PPD-modified sensors showed enhanced selectivity and reproducibility during real-time NO monitoring, particularly in distinguishing NO levels in inflamed versus non-inflamed chondrocyte cultures. The findings highlight the importance of surface modifications to mitigate interference from non-relevant analytes in complex biological environments. These results suggest that real-time NO monitoring with electrochemical sensors could offer a valuable tool for assessing inflammation, with potential future applications in both in vitro and in vivo settings.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137147"},"PeriodicalIF":8.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-30DOI: 10.1016/j.snb.2025.137363
Wiktoria Korona , Barbara Orzechowska , Kacper Siąkała , Anna Maria Nowakowska , Anna Pieczara , Szymon Buda , Robert Pawlowski , Jacek Mlynarski , Malgorzata Baranska
Vibrational techniques using bioorthogonal Raman and infrared (IR) probes that emit unique spectral signals in the silent region (2800–1800 cm⁻¹) have gained considerable attention across various fields of biomedicine. Labeled Raman and infrared microscopies are proving to be highly beneficial in studying the dynamics of metabolic processes within single living cells. Disruption or deregulation of these systems leads to a range of diseases and makes understanding the biochemistry of metabolism one of the greatest challenges in biology. In this study, we built a library of Raman and IR probes to assess metabolic changes, categorizing them into four groups related to glucose and lipid metabolism, as well as the activity of the cell nucleus and mitochondria. We highlight the effectiveness of vibrational labeling in three key areas: tracking specific metabolic pathways, multiplex imaging, and multimodal imaging. Single-pathway analysis was achieved using selected molecular probes (e.g., azido-palmitic acid, EdU, d7-glucose, MitoBADY), each targeting different metabolic pathways. Multiplex imaging allowed for the simultaneous tracking of lipid and mitochondrial metabolism, providing a more comprehensive view of the cell's biomolecular state. Furthermore, a multimodal approach, which combined Raman and optical photothermal infrared (O-PTIR) techniques, enabled the complementary characterization of endothelial cells labeled with azide- and deuterium-palmitic acid. Both high-resolution imaging techniques and microscopy facilitated subcellular analysis in living and fixed cells, also in aqueous environments. These findings underscore the value of integrating vibrational labeling with advanced cell imaging to expand the horizons of metabolomics research.
{"title":"Bioorthogonal Raman and IR probes for live cell metabolomics: A library","authors":"Wiktoria Korona , Barbara Orzechowska , Kacper Siąkała , Anna Maria Nowakowska , Anna Pieczara , Szymon Buda , Robert Pawlowski , Jacek Mlynarski , Malgorzata Baranska","doi":"10.1016/j.snb.2025.137363","DOIUrl":"10.1016/j.snb.2025.137363","url":null,"abstract":"<div><div>Vibrational techniques using bioorthogonal Raman and infrared (IR) probes that emit unique spectral signals in the silent region (2800–1800 cm⁻¹) have gained considerable attention across various fields of biomedicine. Labeled Raman and infrared microscopies are proving to be highly beneficial in studying the dynamics of metabolic processes within single living cells. Disruption or deregulation of these systems leads to a range of diseases and makes understanding the biochemistry of metabolism one of the greatest challenges in biology. In this study, we built a library of Raman and IR probes to assess metabolic changes, categorizing them into four groups related to glucose and lipid metabolism, as well as the activity of the cell nucleus and mitochondria. We highlight the effectiveness of vibrational labeling in three key areas: tracking specific metabolic pathways, multiplex imaging, and multimodal imaging. Single-pathway analysis was achieved using selected molecular probes (e.g., azido-palmitic acid, EdU, d<sub>7</sub>-glucose, MitoBADY), each targeting different metabolic pathways. Multiplex imaging allowed for the simultaneous tracking of lipid and mitochondrial metabolism, providing a more comprehensive view of the cell's biomolecular state. Furthermore, a multimodal approach, which combined Raman and optical photothermal infrared (O-PTIR) techniques, enabled the complementary characterization of endothelial cells labeled with azide- and deuterium-palmitic acid. Both high-resolution imaging techniques and microscopy facilitated subcellular analysis in living and fixed cells, also in aqueous environments. These findings underscore the value of integrating vibrational labeling with advanced cell imaging to expand the horizons of metabolomics research.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137363"},"PeriodicalIF":8.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-30DOI: 10.1016/j.snb.2025.137365
Guochao Chen , Lingzhi Wang , Huan Liu , Lei Wang , Changyuan Yu , Hao Li
Abuse of kanamycin is still present in livestock and poultry farming, thereby causing serious damage to the human body through food chain. Therefore, it is necessary to effectively detect kanamycin in animal sourced food. Here, four different versions of electrochemical aptasensors were developed for efficient detection of kanamycin; especially, a target recycling-based enzyme-free signal dual amplification electrochemical aptasensor (i.e., named as Ver. 4 aptasensor) targeting kanamycin was developed. Under the optimized conditions, the Ver. 4 aptasensor exhibited a high sensitivity toward kanamycin with a limit of detection (LOD) of 0.10 ng/mL (linear range from 0.48 to 4844.99 ng/mL), and a high selectivity against the kanamycin’s structural analogs. Meanwhile, good reusability and excellent detection repeatability further indicated the application feasibility of the Ver. 4 aptasensor. Moreover, the recovery rate of kanamycin from spiked milk samples ranged from 93.24 % to 103.80 %. Results presented here suggested the application potential of the Ver. 4 aptasensor for effectively detecting kanamycin in animal sourced foods.
{"title":"Effective detection of kanamycin residues by target recycling-based enzyme-free signal dual amplification electrochemical aptasensor","authors":"Guochao Chen , Lingzhi Wang , Huan Liu , Lei Wang , Changyuan Yu , Hao Li","doi":"10.1016/j.snb.2025.137365","DOIUrl":"10.1016/j.snb.2025.137365","url":null,"abstract":"<div><div>Abuse of kanamycin is still present in livestock and poultry farming, thereby causing serious damage to the human body through food chain. Therefore, it is necessary to effectively detect kanamycin in animal sourced food. Here, four different versions of electrochemical aptasensors were developed for efficient detection of kanamycin; especially, a target recycling-based enzyme-free signal dual amplification electrochemical aptasensor (<em>i.e.</em>, named as Ver. 4 aptasensor) targeting kanamycin was developed. Under the optimized conditions, the Ver. 4 aptasensor exhibited a high sensitivity toward kanamycin with a limit of detection (LOD) of 0.10 ng/mL (linear range from 0.48 to 4844.99 ng/mL), and a high selectivity against the kanamycin’s structural analogs. Meanwhile, good reusability and excellent detection repeatability further indicated the application feasibility of the Ver. 4 aptasensor. Moreover, the recovery rate of kanamycin from spiked milk samples ranged from 93.24 % to 103.80 %. Results presented here suggested the application potential of the Ver. 4 aptasensor for effectively detecting kanamycin in animal sourced foods.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137365"},"PeriodicalIF":8.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-30DOI: 10.1016/j.snb.2025.137319
Mu Liang , Xuechao Du , Mengya Lu, Shen Tian, Yingying Qiao, Yang Gao, Pengbo Chen, Mingqi Jiao, Lei Li, Chongxin Shan
In this paper, we present for the first time a photoacoustic sensor that uses a fuzzy c-means algorithm based on baseline correction to detect HF gas in the presence of an SF background. This algorithm was proposed to address the issue of unstable amplitude of the second harmonic signal detected by photoacoustic sensors in the near-infrared region due to the weak absorption intensity of gases. The fuzzy c-means algorithm based on baseline correction mainly consists of two parts: firstly, to address the issue of baseline drift in the second harmonic signal, an adaptive iterative reweighted penalty least squares (airPLS) algorithm is employed to remove the drifting baseline, thus stabilizing the signal; secondly, instead of solely relying on the peak value of second harmonic signal for gas concentration calibration, multiple characteristic values of the signal are extracted to form a vector, a fuzzy c-means (FCM) algorithm is then utilized for clustering analysis in a three-dimensional space, using cluster centroids to calibrate gas concentration. The experimental results demonstrate that following algorithmic processing, the R value of the calibration line increases from 0.984 to 0.997, and the minimum detection limit at 1 noise level for HF decreases from 295 ppb to 88 ppb.
{"title":"Ppb-level photoacoustic sensor system for SF6 decomposition component HF using fuzzy c-means algorithm based on baseline correction","authors":"Mu Liang , Xuechao Du , Mengya Lu, Shen Tian, Yingying Qiao, Yang Gao, Pengbo Chen, Mingqi Jiao, Lei Li, Chongxin Shan","doi":"10.1016/j.snb.2025.137319","DOIUrl":"10.1016/j.snb.2025.137319","url":null,"abstract":"<div><div>In this paper, we present for the first time a photoacoustic sensor that uses a fuzzy c-means algorithm based on baseline correction to detect HF gas in the presence of an SF<span><math><msub><mrow></mrow><mrow><mn>6</mn></mrow></msub></math></span> background. This algorithm was proposed to address the issue of unstable amplitude of the second harmonic signal detected by photoacoustic sensors in the near-infrared region due to the weak absorption intensity of gases. The fuzzy c-means algorithm based on baseline correction mainly consists of two parts: firstly, to address the issue of baseline drift in the second harmonic signal, an adaptive iterative reweighted penalty least squares (airPLS) algorithm is employed to remove the drifting baseline, thus stabilizing the signal; secondly, instead of solely relying on the peak value of second harmonic signal for gas concentration calibration, multiple characteristic values of the signal are extracted to form a vector, a fuzzy c-means (FCM) algorithm is then utilized for clustering analysis in a three-dimensional space, using cluster centroids to calibrate gas concentration. The experimental results demonstrate that following algorithmic processing, the R<span><math><msup><mrow></mrow><mrow><mn>2</mn></mrow></msup></math></span> value of the calibration line increases from 0.984 to 0.997, and the minimum detection limit at 1<span><math><mi>σ</mi></math></span> noise level for HF decreases from 295 ppb to 88 ppb.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137319"},"PeriodicalIF":8.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143056389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-30DOI: 10.1016/j.snb.2025.137362
Tingting Liu , Lina Dong , Jing Lv , Bin Ji , Susu Xia , Jiaxin Li , Jing Wang , Jinglin Wang , Jian Zhang , Lihua Tan , Han Jin , Shan Gao , Lin Kang , Wenwen Xin
Abrin, a potent ribosome-inactivating protein, is a serious threat as a biological warfare agent. Developing rapid and accurate assays for detecting abrin are crucial for early prevention of related attacks. In this study, we systematically demonstrate that abrin exhibits apurinic/apyrimidinic (AP) lyase activity, with optimal enzymatic function at 63 °C and pH 4.4. Based on this discovery, we create a fluorescence signaling assay (FSA) for swift detecting abrin and further integrate it with a lateral flow assay (LFA), resulting in a novel, portable detection platform (FSA-LFA). This combined assay allows for sensitive detection of abrin at the picogram level, surpassing the limitations of traditional N-glycosidase-based assays. The FSA-LFA platform is cost-effective, highly sensitive, and amenable to field use. Furthermore, it can be readily adapted for the detection of other ribosome-inactivating proteins, offering broad potential for rapid identification of biosecurity threats.
{"title":"Enhanced detection of abrin: Unraveling AP lyase activity and pioneering innovative assays for advanced biosecurity applications","authors":"Tingting Liu , Lina Dong , Jing Lv , Bin Ji , Susu Xia , Jiaxin Li , Jing Wang , Jinglin Wang , Jian Zhang , Lihua Tan , Han Jin , Shan Gao , Lin Kang , Wenwen Xin","doi":"10.1016/j.snb.2025.137362","DOIUrl":"10.1016/j.snb.2025.137362","url":null,"abstract":"<div><div>Abrin, a potent ribosome-inactivating protein, is a serious threat as a biological warfare agent. Developing rapid and accurate assays for detecting abrin are crucial for early prevention of related attacks. In this study, we systematically demonstrate that abrin exhibits apurinic/apyrimidinic (AP) lyase activity, with optimal enzymatic function at 63 °C and pH 4.4. Based on this discovery, we create a fluorescence signaling assay (FSA) for swift detecting abrin and further integrate it with a lateral flow assay (LFA), resulting in a novel, portable detection platform (FSA-LFA). This combined assay allows for sensitive detection of abrin at the picogram level, surpassing the limitations of traditional <em>N</em>-glycosidase-based assays. The FSA-LFA platform is cost-effective, highly sensitive, and amenable to field use. Furthermore, it can be readily adapted for the detection of other ribosome-inactivating proteins, offering broad potential for rapid identification of biosecurity threats.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137362"},"PeriodicalIF":8.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143056678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-30DOI: 10.1016/j.snb.2025.137364
I‑Ting Peng , Chih-Chia Cheng , Hui-Ling Hsu , Feng-Ping Lin , Chien-Hsing Lu , Jem-Kun Chen
When body fluids come into contact with a fibrous mat, proteins spontaneously adsorb onto the surface, forming a protein corona that masks the original antibody on the surface and significantly reduces the targeting performance. Nylon6 (N6) containing curcumin and a cross-linked protein corona hydrogel were used as the shell and core, respectively, to prepare protein corona-camouflaged immunofiber mats (PIFMs) using coaxial electrospinning for the enrichment of circulating tumor cells (CTCs). A low dosage (1.2 wt%) of curcumin within the N6 reduces the outspread pseudopodia area and enhances the capture capacity of CTCs. The fibrous mat was adhered within a disposable microfluidic chip to investigate the capture capacities of CTCs. The maximum adsorption capacity of CTCs on the PIFMs loaded with curcumin is approximately 26 cells/mm², about 2-fold higher than that on the blank PIFMs (12 cells/mm²). The outer protein corona hydrogel coating not only effectively enhances the antibiofouling properties and cell viability but also significantly decreases hemolysis. In spiked blood samples, our PIFMs loaded with curcumin exhibited a 90.1 % isolation efficiency in a continuous system at a flow rate of 8 mL/hour from the 10 mL blood samples containing 100 CTCs. Our proposed method provides an ideal fibrous mat to embed within a microfluidic chip to analyze CTCs attachment behavior for precise diagnosis in cancer treatment.
{"title":"Disposable microfluidic chip embedded core/shell nylon6/protein corona immunofiber mats for analysis of circulating tumor cell attachment behavior in preclinical trials","authors":"I‑Ting Peng , Chih-Chia Cheng , Hui-Ling Hsu , Feng-Ping Lin , Chien-Hsing Lu , Jem-Kun Chen","doi":"10.1016/j.snb.2025.137364","DOIUrl":"10.1016/j.snb.2025.137364","url":null,"abstract":"<div><div>When body fluids come into contact with a fibrous mat, proteins spontaneously adsorb onto the surface, forming a protein corona that masks the original antibody on the surface and significantly reduces the targeting performance. Nylon6 (N6) containing curcumin and a cross-linked protein corona hydrogel were used as the shell and core, respectively, to prepare protein corona-camouflaged immunofiber mats (PIFMs) using coaxial electrospinning for the enrichment of circulating tumor cells (CTCs). A low dosage (1.2 wt%) of curcumin within the N6 reduces the outspread pseudopodia area and enhances the capture capacity of CTCs. The fibrous mat was adhered within a disposable microfluidic chip to investigate the capture capacities of CTCs. The maximum adsorption capacity of CTCs on the PIFMs loaded with curcumin is approximately 26 cells/mm², about 2-fold higher than that on the blank PIFMs (12 cells/mm²). The outer protein corona hydrogel coating not only effectively enhances the antibiofouling properties and cell viability but also significantly decreases hemolysis. In spiked blood samples, our PIFMs loaded with curcumin exhibited a 90.1 % isolation efficiency in a continuous system at a flow rate of 8 mL/hour from the 10 mL blood samples containing 100 CTCs. Our proposed method provides an ideal fibrous mat to embed within a microfluidic chip to analyze CTCs attachment behavior for precise diagnosis in cancer treatment.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137364"},"PeriodicalIF":8.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143071739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-29DOI: 10.1016/j.snb.2025.137352
Michele Setti , Eoghan Vaughan , Richard Murray , Labrini Sygellou , Aidan J. Quinn , Mauro Riccò , Daniele Pontiroli , Daniela Iacopino
Laser Induced Graphene (LIG) is a highly versatile material with exceptional electrical conductivity and large surface area obtained through the laser pyrolysis of aromatic plastics like polyimides. The recent remarkable discovery that LIG can also be synthesized from environmentally friendly materials like cork, extends application to the manufacture of sustainable, biocompatible, and eco-friendly devices such as biosensors. Here we present the fabrication of a novel “green” non-enzymatic glucose sensor obtained by direct laser writing of flexible cork sheets. To enable glucose detection, the cork sheets were wetted with an aqueous CuSO4 solution. Laser graphitization promoted the conversion of CuSO4 into CuO nanoparticles and resulted in formation of copper-cork Laser Induced Graphene (Cu-cLIG) materials displaying high surface area and high density of CuO NPs embedded in the cLIG matrix. The sensor showed excellent glucose sensing performance in buffer and good selectivity over interfering molecules. A fully laser written sensor was also fabricated and tested for detection of glucose in artificial urine. The sensor exhibited high stability and reproducibility, allowing glucose detection in artificial urine with a high sensitivity of and a LOD of . This easy and eco-friendly fabrication method, combined with the use of renewable and abundant precursor materials, paves the way for the development of truly sustainable sensing platforms for future green electronics.
{"title":"Sustainable electrochemical sensors from cork-derived laser induced graphene: Non-enzymatic glucose detection in urine","authors":"Michele Setti , Eoghan Vaughan , Richard Murray , Labrini Sygellou , Aidan J. Quinn , Mauro Riccò , Daniele Pontiroli , Daniela Iacopino","doi":"10.1016/j.snb.2025.137352","DOIUrl":"10.1016/j.snb.2025.137352","url":null,"abstract":"<div><div>Laser Induced Graphene (LIG) is a highly versatile material with exceptional electrical conductivity and large surface area obtained through the laser pyrolysis of aromatic plastics like polyimides. The recent remarkable discovery that LIG can also be synthesized from environmentally friendly materials like cork, extends application to the manufacture of sustainable, biocompatible, and eco-friendly devices such as biosensors. Here we present the fabrication of a novel “green” non-enzymatic glucose sensor obtained by direct laser writing of flexible cork sheets. To enable glucose detection, the cork sheets were wetted with an aqueous CuSO<sub>4</sub> solution. Laser graphitization promoted the conversion of CuSO<sub>4</sub> into CuO nanoparticles and resulted in formation of copper-cork Laser Induced Graphene (Cu-cLIG) materials displaying high surface area and high density of CuO NPs embedded in the cLIG matrix. The sensor showed excellent glucose sensing performance in buffer and good selectivity over interfering molecules. A fully laser written sensor was also fabricated and tested for detection of glucose in artificial urine. The sensor exhibited high stability and reproducibility, allowing glucose detection in artificial urine with a high sensitivity of <span><math><mrow><mn>223</mn><mspace></mspace><mi>μ</mi><mi>A</mi><mo>/</mo><mrow><mfenced><mrow><mi>mM</mi><mo>⋅</mo><mi>c</mi><msup><mrow><mi>m</mi></mrow><mrow><mn>2</mn></mrow></msup></mrow></mfenced></mrow></mrow></math></span> and a LOD of <span><math><mrow><mn>9.7</mn><mspace></mspace><mi>μ</mi><mi>M</mi></mrow></math></span>. This easy and eco-friendly fabrication method, combined with the use of renewable and abundant precursor materials, paves the way for the development of truly sustainable sensing platforms for future green electronics.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137352"},"PeriodicalIF":8.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143056281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-29DOI: 10.1016/j.snb.2025.137360
PeiJiang Cao , Xin Luo , Fang Jia , YuXiang Zeng , WenJun Liu , DeLiang Zhu , ChunFeng Wang , Shun Han , Ming Fang , XinKe Liu , Dnyandeo Pawar
Transition metal dichalcogenides (TMDCs)-based materials have attracted considerable attention in gas sensing due to their mesmerizing chemical, electrical, optical, and physical properties. In this work, solvothermal synthesized SnSe2 material is well-tuned by thermal treatment to obtain the series of materials such as SnO2/SnSe2, SnO2, & Au-SnO2/SnSe2, and explored for NO2 gas sensing. The SnSe2-36 sensor exhibits a response (Rg/Ra) of 5.5 under 4 ppm NO2 at 30 °C. The sensing performance is significantly enhanced for SnO2/SnSe2-1 heterojunction with a response of 15.07, response/recovery time of 63 s /122 s, and limit of detection (LOD) of 60.61 ppb to 4 ppm NO2 at 90 °C. The remarkable sensing performance is observed for Au-SnO2/SnSe2-1 heterojunction with a response of 25.31, response/recovery time of 156 s/56 s, and LOD of 13.67 ppb to 4 ppm NO2 at 80 °C. The improvement in the gas sensing properties of the materials is attributed to the good hoping of electron exchange between the depletion layer and NO2 gas, increase in specific surface area at the interface, lower adsorption energy of NO2 with heterojunction, and spill-over effect. The remarkable results could attract key attention in materials synthesis and its wide tunability towards gas sensing applications.
{"title":"Highly sensitive, selective, and ppb level detection of NO2 gas using SnSe2 micro-flower, SnO2/SnSe2, and Au-SnO2/SnSe2 heterojunctions","authors":"PeiJiang Cao , Xin Luo , Fang Jia , YuXiang Zeng , WenJun Liu , DeLiang Zhu , ChunFeng Wang , Shun Han , Ming Fang , XinKe Liu , Dnyandeo Pawar","doi":"10.1016/j.snb.2025.137360","DOIUrl":"10.1016/j.snb.2025.137360","url":null,"abstract":"<div><div>Transition metal dichalcogenides (TMDCs)-based materials have attracted considerable attention in gas sensing due to their mesmerizing chemical, electrical, optical, and physical properties. In this work, solvothermal synthesized SnSe<sub>2</sub> material is well-tuned by thermal treatment to obtain the series of materials such as SnO<sub>2</sub>/SnSe<sub>2</sub>, SnO<sub>2</sub>, & Au-SnO<sub>2</sub>/SnSe<sub>2</sub>, and explored for NO<sub>2</sub> gas sensing. The SnSe<sub>2</sub>-36 sensor exhibits a response (R<sub>g</sub>/R<sub>a</sub>) of 5.5 under 4 ppm NO<sub>2</sub> at 30 °C. The sensing performance is significantly enhanced for SnO<sub>2</sub>/SnSe<sub>2</sub>-1 heterojunction with a response of 15.07, response/recovery time of 63 s /122 s, and limit of detection (LOD) of 60.61 ppb to 4 ppm NO<sub>2</sub> at 90 °C. The remarkable sensing performance is observed for Au-SnO<sub>2</sub>/SnSe<sub>2</sub>-1 heterojunction with a response of 25.31, response/recovery time of 156 s/56 s, and LOD of 13.67 ppb to 4 ppm NO<sub>2</sub> at 80 °C. The improvement in the gas sensing properties of the materials is attributed to the good hoping of electron exchange between the depletion layer and NO<sub>2</sub> gas, increase in specific surface area at the interface, lower adsorption energy of NO<sub>2</sub> with heterojunction, and spill-over effect. The remarkable results could attract key attention in materials synthesis and its wide tunability towards gas sensing applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137360"},"PeriodicalIF":8.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143055266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lanthanide metal-organic frameworks (Ln-MOFs) have emerged as promising electrochemiluminescence (ECL) luminophores on account of their distinctive luminescence performances. However, currently reported Ln-MOFs-based ECL luminophores are mainly limited to terbium-based MOFs (Tb-MOFs) and europium-based MOFs (Eu-MOFs). This work innovatively developed samarium-based MOFs (Sm-MOFs) as ECL luminophores. Sm-MOFs were prepared via hydrothermal method with Sm3 + as metal node and 1,3,5-benzenetricarboxylic acid (H3BTC) as organic ligand. H3BTC as “antenna” transferred energy to Sm3+ through antenna effect, enabling Sm-MOFs to emit brilliant cathode ECL signal with persulfate (S2O82-) as coreactant. Bismuth sulfide (Bi2S3) acted as signal regulator to remarkably quench the ECL emission from Sm-MOFs through ECL resonance energy transfer (ECL-RET). Using acetamiprid (ACE) as the target detection model, the ECL luminophores Sm-MOFs cleverly coupled signal regulator Bi2S3 and enzyme-free triple amplification strategy of hybridization chain reaction (HCR), catalytic hairpin assembly (CHA), and DNAzyme walker to fabricate an ECL biosensor. The limit of detection (LOD) was as low as 4.58 fM. This work not only exploited novel Ln-MOFs-based ECL luminophores and their application in ECL field, but also built an enzyme-free multiple amplification strategy for highly sensitive assay of ACE.
{"title":"Novel samarium-based metal-organic frameworks with antenna effect-induced electrochemiluminescence for acetamiprid assay","authors":"Xiaoge Peng , Ying He , Kejun Tan, Ruo Yuan, Shihong Chen","doi":"10.1016/j.snb.2025.137343","DOIUrl":"10.1016/j.snb.2025.137343","url":null,"abstract":"<div><div>Lanthanide metal-organic frameworks (Ln-MOFs) have emerged as promising electrochemiluminescence (ECL) luminophores on account of their distinctive luminescence performances. However, currently reported Ln-MOFs-based ECL luminophores are mainly limited to terbium-based MOFs (Tb-MOFs) and europium-based MOFs (Eu-MOFs). This work innovatively developed samarium-based MOFs (Sm-MOFs) as ECL luminophores. Sm-MOFs were prepared via hydrothermal method with Sm<sup>3 +</sup> as metal node and 1,3,5-benzenetricarboxylic acid (H<sub>3</sub>BTC) as organic ligand. H<sub>3</sub>BTC as “antenna” transferred energy to Sm<sup>3+</sup> through antenna effect, enabling Sm-MOFs to emit brilliant cathode ECL signal with persulfate (S<sub>2</sub>O<sub>8</sub><sup>2-</sup>) as coreactant. Bismuth sulfide (Bi<sub>2</sub>S<sub>3</sub>) acted as signal regulator to remarkably quench the ECL emission from Sm-MOFs through ECL resonance energy transfer (ECL-RET). Using acetamiprid (ACE) as the target detection model, the ECL luminophores Sm-MOFs cleverly coupled signal regulator Bi<sub>2</sub>S<sub>3</sub> and enzyme-free triple amplification strategy of hybridization chain reaction (HCR), catalytic hairpin assembly (CHA), and DNAzyme walker to fabricate an ECL biosensor. The limit of detection (LOD) was as low as 4.58 fM. This work not only exploited novel Ln-MOFs-based ECL luminophores and their application in ECL field, but also built an enzyme-free multiple amplification strategy for highly sensitive assay of ACE.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"430 ","pages":"Article 137343"},"PeriodicalIF":8.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143056391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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