Sensors and Actuators B: Chemical最新文献_第6页

A comprehensive diagnostic platform leveraging voice-control feature for rapid SARS-CoV-2 detection using reverse transcription loop-mediated isothermal amplification

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-25 DOI: 10.1016/j.snb.2025.137690

Phuc Hoang Dinh, Tae Seok Seo

The COVID-19 pandemic has significantly spurred the development and innovation of the point-of-care testing (POCT) field, highlighting the urgent need for rapid, accurate, and accessible diagnostic tools. Integration of contemporary technologies, such as the Internet of Things (IoT) and speech recognition, can greatly enhance the functionality and usability of POCT devices. Herein, we constructed a voice-controllable molecular diagnostic platform for rapid SARS-CoV-2 diagnosis using reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. The platform is a complete system comprised of a POCT device, a microfluidic chip, and a web app developed for smartphones. The microfluidic chip, designed with pneumatic microvalves, enables automatic dispensing of the RT-LAMP mixture into four reaction chambers for amplifying and analyzing different target genes of SARS-CoV-2, ensuring accurate diagnostic results. The POCT device has a compact design including modules for valve control, thermal regulation, fluid manipulation, and real-time fluorescence detection. The smartphone web app integrates the speech recognition feature for voice-controllable operations and provides an intuitive interface for displaying and interpreting results in real time. Featuring sophisticated algorithms for real-time dynamic C_t calculation and advanced image processing, the platform detects SARS-CoV-2 with a low limit-of-detection (LOD) of 10⁰ genomic copies/μL within 35 min with high specificity. By leveraging the IoT for wireless data control and transfer, coupled with a speech-recognition feature, the platform offers hands-free operation to streamline the diagnostic process and minimize potential contamination risks while enhancing user convenience.

{"title":"A comprehensive diagnostic platform leveraging voice-control feature for rapid SARS-CoV-2 detection using reverse transcription loop-mediated isothermal amplification","authors":"Phuc Hoang Dinh, Tae Seok Seo","doi":"10.1016/j.snb.2025.137690","DOIUrl":"10.1016/j.snb.2025.137690","url":null,"abstract":"<div><div>The COVID-19 pandemic has significantly spurred the development and innovation of the point-of-care testing (POCT) field, highlighting the urgent need for rapid, accurate, and accessible diagnostic tools. Integration of contemporary technologies, such as the Internet of Things (IoT) and speech recognition, can greatly enhance the functionality and usability of POCT devices. Herein, we constructed a voice-controllable molecular diagnostic platform for rapid SARS-CoV-2 diagnosis using reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. The platform is a complete system comprised of a POCT device, a microfluidic chip, and a web app developed for smartphones. The microfluidic chip, designed with pneumatic microvalves, enables automatic dispensing of the RT-LAMP mixture into four reaction chambers for amplifying and analyzing different target genes of SARS-CoV-2, ensuring accurate diagnostic results. The POCT device has a compact design including modules for valve control, thermal regulation, fluid manipulation, and real-time fluorescence detection. The smartphone web app integrates the speech recognition feature for voice-controllable operations and provides an intuitive interface for displaying and interpreting results in real time. Featuring sophisticated algorithms for real-time dynamic C<sub>t</sub> calculation and advanced image processing, the platform detects SARS-CoV-2 with a low limit-of-detection (LOD) of 10<sup>0</sup> genomic copies/μL within 35min with high specificity. By leveraging the IoT for wireless data control and transfer, coupled with a speech-recognition feature, the platform offers hands-free operation to streamline the diagnostic process and minimize potential contamination risks while enhancing user convenience.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"436 ","pages":"Article 137690"},"PeriodicalIF":8.0,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143703484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

"Molecular Velcro": Design of coupled AuNPs with streptavidin-biotin immobilized nanobody in lateral flow immunoassay for sensitive Salmonella typhimurium detection

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-24 DOI: 10.1016/j.snb.2025.137604

Jiamin Wang, Longhua Shi, Yinnan Jing, Xiatong Wang, Xuan Liu, Shurong Li, Qinkai Yang, Jianlong Wang, Yanru Wang

The synthesis of nanoparticle-nanobody (Nb) conjugates exhibiting high stability and affinity remains challenging in the development of lateral flow immunoassay (LFIA). Herein, we utilized the streptavidin-biotin system as a “Molecular Velcro” to enhance the bioactivity of nanogold-Nb probes through oriented conjugation of biotinylated nanobodies with streptavidin-coated gold nanoparticles. The Au/SA@Bio-Nb probe presented higher stability and affinity. On this basis, an improved LFIA was established for rapid and sensitive detection of S. typhimurium in food. The visual detection limit of the Au/SA@Bio-Nb-LFIA is 10³ CFU mL⁻¹, and the linear detection range is 10³–10⁷ CFU mL⁻¹. In addition, the recovery rate of the portable immunosensor for detecting S. typhimurium in food samples is 81.23 %–105.01 %. Overall, this study provides great promise for the design and preparation of Nb-based probes to enhance the detection performance of LFIA methods.

合成具有高稳定性和高亲和力的纳米颗粒-纳米抗体（Nb）共轭物仍然是横向流动免疫分析（LFIA）开发过程中的一项挑战。在此，我们利用链霉亲和素-生物素系统作为 "分子魔术贴"，通过生物素化纳米抗体与链霉亲和素包覆金纳米粒子的定向共轭，增强了纳米金-纳米抗体探针的生物活性。Au/SA@Bio-Nb 探针具有更高的稳定性和亲和力。在此基础上，建立了一种改进的 LFIA，用于快速灵敏地检测食品中的鼠伤寒杆菌。Au/SA@Bio-Nb-LFIA 的肉眼检测限为 103 CFU mL-1，线性检测范围为 103-107 CFU mL-1。此外，便携式免疫传感器检测食品样品中伤寒杆菌的回收率为 81.23%-105.01%。总之，这项研究为设计和制备基于 Nb 的探针以提高 LFIA 方法的检测性能提供了广阔的前景。

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引用次数: 0

Ratiometric fluorometry on microfluidic paper-based analytical device for simultaneous glucose and cholesterol detection using MnFe-layered double hydroxides as peroxidase mimic

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-24 DOI: 10.1016/j.snb.2025.137671

Nattasa Kitchawengkul , Akarapong Prakobkij , Rattaporn Saenmuangchin , Daniel Citterio , Duangjai Nacapricha , Purim Jarujamrus

A highly sensitive ratiometric fluorescence sensing system was developed for simultaneous glucose and total cholesterol (TC) detection in whole blood using MnFe-layered double hydroxides (MnFe-LDHs) as a peroxidase mimic, combined with an o-phenylenediamine (OPD) substrate and nitrogen-doped graphene quantum dots (N-GQDs). The detection platform, an X-shaped laminated microfluidic paper-based analytical device (XL-μPAD), was fabricated via laser printing and cutting. The MnFe-LDHs' large surface area and layered structure provide a high affinity for OPD, with a Michaelis–Menten constant (K_M) of 0.0127 mmol L⁻¹. Upon placing a drop of blood on the XL-μPAD sample pad, the enzymatic reactions of glucose and TC produce H₂O₂, which MnFe-LDHs convert to hydroxyl radicals (^•OH). These radicals oxidize OPD into fluorescent 2,3-diamino phenazine (DAP) with emission at 560 nm. Meanwhile, the N-GQDs emit fluorescence at 415 nm, which is quenched by DAP through the inner filter effect (IFE) and dynamic quenching, enabling ratiometric sensing via the intensity ratio (I₅₆₀/I₄₁₅). As H₂O₂ levels increase, a visible green emission appears, correlating with glucose and TC levels. This XL-μPAD system demonstrates promising potential as a portable device for multiplex biomarker detection and diagnostic applications.

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引用次数: 0

Rapid and ultra-sensitive detection of foodborne pathogens by deep learning-enhanced microfluidic biosensing

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-24 DOI: 10.1016/j.snb.2025.137646

Haiyan Ma , Guojie Li , Huihui Zhang , Xinyu Wang , Fengyun Li , Jing Yan , Liu Hong , Yuewen Zhang , Qiaosheng Pu

Rapid and sensitive detection of foodborne pathogens is essential for ensuring food safety and protecting public health. In this study, we developed an innovative microfluidic fluorescence digital analysis platform enhanced by deep learning to detect pathogens at ultra-low concentrations. The biosensor features a staggered herringbone double-spiral (SHDS) microfluidic design, seamlessly integrating bacteria capture, detection, and release processes using Quantum dot (QD)-Aptamer conjugates for precise identification. Fluorescence image analysis, powered by a Resnet-18-based convolutional neural networks (CNN), directly quantifies Escherichia coli (E. coli) concentrations from fluorescence images, streamlining data processing and increasing sensitivity. The platform offers a linear detection range from 10 to 3 × 10⁶ CFU/mL (R² = 0.990), achieves capture efficiencies of up to 100 % at low bacterial concentrations (4 × 10² CFU/mL), and offers an ultra-low detection limit of 2 CFU/mL within just 1.5 hours. The CNN model effectively filters out background noise and interferences, achieving over 99 % predictive accuracy. Validation using milk and chicken samples resulted in high recovery rates (96.7 % to 104.0 %). This biosensor presents a rapid, reliable, and practical solution for pathogen detection in complex food matrices, significantly improving food safety and security.

快速灵敏地检测食源性病原体对于确保食品安全和保护公众健康至关重要。在这项研究中，我们开发了一种创新的微流控荧光数字分析平台，该平台通过深度学习增强了对超低浓度病原体的检测能力。该生物传感器采用交错人字形双螺旋（SHDS）微流体设计，无缝集成了细菌捕获、检测和释放过程，使用量子点（QD）-色聚体共轭物进行精确识别。荧光图像分析由基于 Resnet-18 的卷积神经网络 (CNN) 支持，可直接量化荧光图像中的大肠杆菌浓度，从而简化数据处理并提高灵敏度。该平台的线性检测范围从 10 到 3 × 10⁶ CFU/mL（R² = 0.990），在细菌浓度较低（4 × 10² CFU/mL）时捕获效率高达 100%，并在 1.5 小时内达到 2 CFU/mL 的超低检测限。CNN 模型能有效过滤背景噪声和干扰，预测准确率超过 99%。使用牛奶和鸡肉样品进行验证后，回收率高达 96.7% 至 104.0%。这种生物传感器为复杂食品基质中的病原体检测提供了一种快速、可靠和实用的解决方案，大大提高了食品安全和保障水平。

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引用次数: 0

UV enhanced SnO2/TiO2 nanorods-based flexible room temperature sensor by tuning interfacial chemistry and microstructure

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-24 DOI: 10.1016/j.snb.2025.137680

X.L. Xu , M.Y. Wang , H.T. Jiang , W.W. Liu, G.R. Sun, W. Ma

The high working temperature of metal oxide semiconductors hinders its application in flexible sensing devices. In this paper, a UV-activated flexible sensor operating at room temperature (RT, 25℃) is first reported based on SnO₂ nanoparticles embedded in photo-catalytic TiO₂ nanorods matrix. The UV-irradiated SnO₂/TiO₂ sensor exhibits excellent selectivity (109 ppm, 37 %) to CH₃COOH and reduced response/recovery time (12 s/41 s). The excellent RT flexible sensing properties are attributed to the synergistic action of UV-365 nm irradiation, the hetero-embedded microstructure and innovative terpineol binder. UV irradiation generates substantial

e_{h υ}^{-}

-

h_{h υ}^{+}

pairs, which serve as active sites to promote the chemisorption and redox reactions. Additional activation energy helps to improve adsorption/desorption kinetics. The heterojunction provides abundant channels, facilitating the separation and transfer of photogenerated carriers. The effect of relative humidity (RH) and temperature variations around RT on the sensor response towards CH₃COOH are investigated. Under low-RH (35 %-45 %) and high-RH conditions (above 45 %), 1 % RH change has the same effect on the sensor response than 7.2 ppm and 59.4 ppm CH₃COOH, respectively. 1°C temperature change exhibits the same effect than 17.9 ppm CH₃COOH. Such a noise effect is by far not suitable for real-world applications. In addition, the response to 109 ppm CO reaches up to 89.5. Therefore, the sensor is seriously disturbed by CO and further research is needed. Above all, UV assistants, hetero-composites combining with innovative microstructure design are expected to be a collaborative enhancement strategy for MOSs-based RT flexible sensors.

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引用次数: 0

Self-powered photoelectrochemical immunosensor using MIL-88A-derived NiFe LDH double-shell nanocages/TiO2/P-doped graphitic carbon nitride heterostructure for hCG detection in urine samples of pregnant and non-pregnant women 利用 MIL-88A 衍生的 NiFe LDH 双壳纳米笼/TiO2/P掺杂石墨氮化碳异质结构自供电光电化学免疫传感器检测孕妇和非孕妇尿样中的 hCG

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-23 DOI: 10.1016/j.snb.2025.137670

Rajalakshmi Sakthivel , Yu-Ting Liao , Subbiramaniyan Kubendhiran , Yu-Chien Lin , Lu-Yin Lin , Yeh-Fang Duann , Da-Hua Wei , Ren-Jei Chung

Human chorionic gonadotropin (hCG) is an essential biomarker for pregnancy and seminal system disorders, with elevated levels linked to tumor progression. Early and accurate detection of hCG is critical for disease diagnosis, necessitating the development of highly sensitive and selective assays. Self-powered photoelectrochemical (PEC) biosensors, which function without an external power source at zero bias voltage, offer superior anti-interference properties and have emerged as a promising approach for biomarker detection. This study presents the synthesis of MIL-88A-derived NiFe LDH double-shell nanocages integrated with titanium dioxide (TiO₂) and phosphorus-doped graphitic carbon nitride (PCN). The resulting Ni-Fe LDH DSNCs/TiO₂/PCN heterostructure was immobilized on a fluorine-doped tin oxide (FTO) substrate for efficient hCG detection under zero-bias conditions (0 V vs. Ag/AgCl). Ascorbic acid (AA) acts as an electron donor to scavenge photogenerated holes, thereby improving the sensitivity of the PEC immunosensor. Compared to bare FTO, the Ni-Fe LDH DSNCs/TiO₂/PCN heterostructure exhibited a significantly higher PEC signal, attributed to its enhanced photoelectric conversion efficiency, lower charge recombination, and effective charge separation. The PEC immunosensor shows a linear response to hCG (0.0001–100 ng/mL) with a detection limit of 0.087 pg/mL. The superior performance of the Ni-Fe LDH DSNCs/TiO₂/PCN-based PEC immunosensor can be attributed to its optimized bandgap, efficient charge transport, broad visible-light absorption, and large surface area. Furthermore, the PEC sensor exhibited excellent stability, selectivity, and reproducibility, with high recovery rates in real urine sample analysis, demonstrating its potential for reliable hCG detection.

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引用次数: 0

Millimeter-wave measurements in high finesse cavity of nitro-derivatives traces: A new insight in the explosive vapor sensing

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-23 DOI: 10.1016/j.snb.2025.137629

Mhamad Chrayteh , Fabien Simon , Francis Hindle , Gaël Mouret , Anthony Roucou , Manuel Goubet , Julien Mory , Christelle Nicollet , Arnaud Cuisset

Cavity-Enhanced Absorption Spectroscopy (CEAS) and Cavity Ring-Down Spectroscopy (CRDS) are well established for sensitive infrared measurements of gas-phase compounds at trace levels using their rovibrational signatures. The recent successful development of a THz Fabry–Perot spectrometer by Hindle et al. (2019) shows that the adaptation of such techniques to submillimeter wavelengths allows to probe rotational transitions of light polar compounds. Here we report on the development of a new millimeter-wave resonator, covering the 150–215 GHz frequency range, and based on a low-loss corrugated waveguide with homemade highly reflective photonic mirrors obtaining a finesse above 3000 at around 164 GHz. With an effective path length of two kilometers, a significant sensitivity has been evaluated, and the detection of semi-volatile organic vapors at a trace level may be now envisaged at room temperature. We applied this technology to detect gas-phase explosive taggants and precursors, confirming a detection limit of 2 ppmv for nitromethane (NM). Leveraging the unique characteristics of the millimeter wave frequency band, we showcase highly selective detection in quasi-realistic environments of complex chemical mixtures involving explosive taggants with close chemical structures such as nitrotoluene isomers. Furthermore, we successfully address the challenge of detecting these nitro-derivative compounds vaporized from model matrices: KCl matrices, granular and plastic (NP91) explosives respectively in conventional and pyrotechnic laboratories. Our findings underscore this approach as a potent tool for practical explosive detection applications.

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引用次数: 0

A portable all-in-one microfluidic platform integrated with CRISPR-based extraction-free assay for rapid and on-site detection of monkeypox and lumpy skin disease

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-22 DOI: 10.1016/j.snb.2025.137612

Yizheng Huang , Yuhan Lu , Xiaofei Liu , Menghao Chai , Ling Yang , Kun Yin , Jiayao He , Zhijie Wang , Yajun Zhang , Yude Yu , Songyin Qiu , Yiqiang Fan , Zhao Li

Rapid and accurate identification of infectious disease pathogens plays a key role in emergency preparedness and respond to a wide range of biosecurity threats. However, current strategies require dedicated laboratories, expensive instruments and skilled operators, which are not suitable for point-of-care testing. Here, we developed an integrated CRISPR-based microfluidic platform that consisted of 3D printed bioreactors and a homemade miniaturized heater for rapid and sensitive nucleic acids detection. The all-in-one, containment-free bioreactors combined recombinase polymerase amplification, CRISPR and lateral flow dipsticks (RPA-CRISPR-LFD), allowing user-friendly operation and eliminating the need for complex laboratory equipment. The RPA-CRISPR-LFD assay was able to efficiently detect plasmids of monkeypox virus (MPXV) and lumpy skin disease virus (LSDV) down to 1 copy

μ L^{- 1}

. The platform can identify 5 extraction-free samples in parallel within 30 min, significantly reducing time consumption. In addition, we validated its clinical utility and versatility by testing 67 simulated blood or swab samples of MPXV and LSDV, yielding results consistent with real-time PCR. Thus, the platform enables rapid and convenient detection of diverse pathogens, and with the future integration of lyophilized reagents, it offers a promising tool for point-of-care testing of major biosecurity threats, especially in source-limited areas.

快速准确地识别传染病病原体在应急准备和应对各种生物安全威胁方面发挥着关键作用。然而，目前的策略需要专门的实验室、昂贵的仪器和熟练的操作人员，不适合进行床旁检测。在这里，我们开发了一种基于CRISPR技术的集成微流控平台，该平台由3D打印生物反应器和自制微型加热器组成，用于快速灵敏地检测核酸。这种一体化、无封闭性的生物反应器将重组酶聚合酶扩增、CRISPR 和侧流浸渍棒（RPA-CRISPR-LFD）结合在一起，操作方便，无需复杂的实验室设备。RPA-CRISPR-LFD 分析法能够有效地检测猴痘病毒（MPXV）和块状皮肤病病毒（LSDV）的质粒，最低可达到 1 拷贝 μL-1μL-1。该平台可在 30 分钟内同时鉴定 5 份免提取样本，大大减少了时间消耗。此外，我们还对 67 份 MPXV 和 LSDV 的模拟血液或拭子样本进行了检测，结果与实时 PCR 一致，从而验证了该平台的临床实用性和多功能性。因此，该平台能快速、方便地检测各种病原体，未来还将整合冻干试剂，为主要生物安全威胁的床旁检测提供了一种前景广阔的工具，尤其是在来源有限的地区。

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引用次数: 0

Expand detection windows for identifying single nucleotide polymorphisms using a competitive toehold-mediated strand displacement ratiometric sensing platform

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-22 DOI: 10.1016/j.snb.2025.137666

Yunshan Zhang , Sisi Bu , Fang Yang , Tuo Huang , Hao Dong , Jing Ye , Wenlin Xie , Xianzhong Feng , Diming Zhang

The subtle free energy difference introduced by a single nucleotide mutation results in poor specificity of almost all DNA hybridization probe-based single nucleotide polymorphism (SNP) detection techniques. The development of SNP biosensing strategies with both specificity and sensitivity is a hot and difficult issue in the current field. In this study, we creatively constructed a competitive toehold-mediated strand displacement sensing platform (CTMSD) based on the traditional TMSD reaction, which increased the energy barrier through the intrinsic competition mechanism and expanded the detection window of SNPs. Furthermore, based on the characteristics of the CTMSD platform, the dual-signal detection mode was introduced to change the function model of the detection curve through reporting internal reference ratio signal. The new detection curve model not only compensated for sensitivity, significantly enhanced the discrimination factor, but also greatly expanded the detection window with infinite robustness factor over the detection range. The expansion of the detection window and the improvement of specificity of CTMSD for SNP recognition based on the ratiometric signal output model were verified by computer simulations and experiments. In addition, as a deformation of the strand displacement reaction, the CTMSD was readily adaptable to commonly used signal amplification techniques, such as catalytic hairpin assembly (CHA). Through the CTMSD-CHA performance analysis and real testing of cell genomic samples, the practical application value of CTMSD with the ratiometric signal output model was confirmed. This study provides an important reference for the design and improvement of SNP biosensors and even for all nucleic acid biosensors.

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引用次数: 0

Screening and diagnosis of colorectal cancer using nucleic acid aptamers targeting Solobacterium moorei: Development of electrochemical sensors for clinical application 利用以莫雷溶杆菌为靶标的核酸适配体筛查和诊断结直肠癌：开发用于临床应用的电化学传感器

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-22 DOI: 10.1016/j.snb.2025.137669

Decai Yuan , Cheng Qiu , Hui Chen , Feng Liu , Shanshan Feng , Peiyi Zhang , Ying Qin , Tingting Fan , Yan Chen , Yuyang Jiang

Colorectal cancer (CRC) represents a significant global health issue, necessitating innovative approaches for early screening and diagnosis. Recent advances in molecular diagnostics have highlighted the potential of aptamers for use as highly specific and sensitive probes for detecting cancer biomarkers. In this study, we focus on the identification of aptamers that selectively bind to Solobacterium moorei (S. moorei), a bacterium associated with CRC. By integrating these aptamers into electrochemical sensor platforms, a reliable diagnostic tool is created for facile implementation in the clinical setting. More specifically, nucleic acid aptamers for S. moorei were obtained through whole-cell SELEX, and the affinity and specificity of these aptamers were validated. Subsequently, two types of electrochemical sensors were developed. Firstly, an electrochemical impedance spectroscopy sensor was developed to detect impedance changes on the electrode surface, which were caused by the binding of S. moorei to the aptamer. Secondly, a CRISPR/Cas12a-based electrochemical aptasensor was developed based on the ability of the Cas12a enzyme to be activated by specific single-stranded DNA, triggering its trans-cleavage activity. The limits of detection of these sensors for S. moorei were 30 and 6 CFU/mL, respectively. Clinical validation was performed using patient samples to assess the sensor efficacy in a real-world setting. The obtained results suggested that the abundance of S. moorei in the feces of CRC patients was significantly greater compared to that of healthy individuals. This integration of S. moorei aptamers into electrochemical sensors offers a non-invasive and cost-effective alternative to current screening methods available for CRC.

{"title":"Screening and diagnosis of colorectal cancer using nucleic acid aptamers targeting Solobacterium moorei: Development of electrochemical sensors for clinical application","authors":"Decai Yuan , Cheng Qiu , Hui Chen , Feng Liu , Shanshan Feng , Peiyi Zhang , Ying Qin , Tingting Fan , Yan Chen , Yuyang Jiang","doi":"10.1016/j.snb.2025.137669","DOIUrl":"10.1016/j.snb.2025.137669","url":null,"abstract":"<div><div>Colorectal cancer (CRC) represents a significant global health issue, necessitating innovative approaches for early screening and diagnosis. Recent advances in molecular diagnostics have highlighted the potential of aptamers for use as highly specific and sensitive probes for detecting cancer biomarkers. In this study, we focus on the identification of aptamers that selectively bind to <em>Solobacterium moorei (S. moorei)</em>, a bacterium associated with CRC. By integrating these aptamers into electrochemical sensor platforms, a reliable diagnostic tool is created for facile implementation in the clinical setting. More specifically, nucleic acid aptamers for <em>S. moorei</em> were obtained through whole-cell SELEX, and the affinity and specificity of these aptamers were validated. Subsequently, two types of electrochemical sensors were developed. Firstly, an electrochemical impedance spectroscopy sensor was developed to detect impedance changes on the electrode surface, which were caused by the binding of <em>S. moorei</em> to the aptamer. Secondly, a CRISPR/Cas12a-based electrochemical aptasensor was developed based on the ability of the Cas12a enzyme to be activated by specific single-stranded DNA, triggering its trans-cleavage activity. The limits of detection of these sensors for <em>S. moorei</em> were 30 and 6 CFU/mL, respectively. Clinical validation was performed using patient samples to assess the sensor efficacy in a real-world setting. The obtained results suggested that the abundance of <em>S. moorei</em> in the feces of CRC patients was significantly greater compared to that of healthy individuals. This integration of <em>S. moorei</em> aptamers into electrochemical sensors offers a non-invasive and cost-effective alternative to current screening methods available for CRC.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"436 ","pages":"Article 137669"},"PeriodicalIF":8.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0