Sensors and Actuators B: Chemical最新文献

英文中文

The surface plasmon resonance aptasensor based on the dual-interface Mott−Schottky heterojunction of Zr-doped MnB4 nanosheets decorated with gold nanoparticles for sensitively detecting nucleolin

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-05 DOI: 10.1016/j.snb.2025.137721

Chuanpan Guo , Yihan He , Kaige Chen , Shuai Zhang , Minghua Wang , Linghao He , Zhihong Zhang

In this work, we have designed and developed a novel surface plasmon resonance (SPR) aptasensor based on the two-dimensional (2D) Zr-doped MnB₄ nanosheets decorated with Au nanoparticles (NPs) (denoted as AuNPs@Zr/MnB₄) for efficiently detecting nucleolin (NCL).The established AuNPs@Zr/MnB₄ dual-interface Schottky heterojunction benefitted from the modulatory effect of the density state of Mn sites in MnB₄ through Zr doping and the incorporation of Au NPs, endowing it with high optical absorption efficiency, fast charge carrier mobility, and improved work function. Thus, it exhibited enhanced interface electric field energy of the surface plasmon effect, amplifying SPR response. Moreover, the rich Zr sites in AuNPs@Zr/MnB₄ considerably promoted the immobilization of large amounts of AS1411 aptamer strands through the Zr–O–P covalent bond, coordination effect, and van der Waal force, rendering the high detection sensitivity to NCL. The AuNPs@Zr/MnB₄-based SPR aptasensor demonstrated a wide dynamic range (1.0 ag mL⁻¹ to 100 ng mL⁻¹) and an ultralow detection limit (0.44 ag mL⁻¹). This work introduced a new strategy for the construction of SPR aptasensors to enable the sensitive inspection of biomarkers and extended the application of 2D MBene nanomaterials in biosensing and biological diagnosis.

{"title":"The surface plasmon resonance aptasensor based on the dual-interface Mott−Schottky heterojunction of Zr-doped MnB4 nanosheets decorated with gold nanoparticles for sensitively detecting nucleolin","authors":"Chuanpan Guo , Yihan He , Kaige Chen , Shuai Zhang , Minghua Wang , Linghao He , Zhihong Zhang","doi":"10.1016/j.snb.2025.137721","DOIUrl":"10.1016/j.snb.2025.137721","url":null,"abstract":"<div><div>In this work, we have designed and developed a novel surface plasmon resonance (SPR) aptasensor based on the two-dimensional (2D) Zr-doped MnB<sub>4</sub> nanosheets decorated with Au nanoparticles (NPs) (denoted as AuNPs@Zr/MnB<sub>4</sub>) for efficiently detecting nucleolin (NCL).The established AuNPs@Zr/MnB<sub>4</sub> dual-interface Schottky heterojunction benefitted from the modulatory effect of the density state of Mn sites in MnB<sub>4</sub> through Zr doping and the incorporation of Au NPs, endowing it with high optical absorption efficiency, fast charge carrier mobility, and improved work function. Thus, it exhibited enhanced interface electric field energy of the surface plasmon effect, amplifying SPR response. Moreover, the rich Zr sites in AuNPs@Zr/MnB<sub>4</sub> considerably promoted the immobilization of large amounts of AS1411 aptamer strands through the Zr–O–P covalent bond, coordination effect, and van der Waal force, rendering the high detection sensitivity to NCL. The AuNPs@Zr/MnB<sub>4</sub>-based SPR aptasensor demonstrated a wide dynamic range (1.0 ag mL<sup>−1</sup> to 100 ng mL<sup>−1</sup>) and an ultralow detection limit (0.44 ag mL<sup>−1</sup>). This work introduced a new strategy for the construction of SPR aptasensors to enable the sensitive inspection of biomarkers and extended the application of 2D MBene nanomaterials in biosensing and biological diagnosis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"437 ","pages":"Article 137721"},"PeriodicalIF":8.0,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly sensitive and fast response/recovery ammonia sensor based on PANI/LIG at room temperature 室温下基于 PANI/LIG 的高灵敏度、快速响应/回收氨气传感器

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-04 DOI: 10.1016/j.snb.2025.137710

Li Yang , Longbiao Mao , Shuaijie Du , Zihan Wang , Wenyuan Fu , Chengpeng Yao , Luxiang Xu , Hui Zhang , Huanyu Cheng

Real-time and accurate detection of ammonia in the environment provides timely alarm for excessive exposure or food spoilage. However, metal oxide-based ammonia gas sensors often need to operate at high temperatures (200–400℃) and conductive polymer-based ammonia gas sensors suffer from long response/recovery time, which are challenging for real-time wearable applications. Meanwhile, three-dimensional porous laser-induced graphene (LIG) features simple preparation, high carrier mobility, good conductivity, and large specific surface area, presenting opportunities to further combine with conductive polymers for enhanced performance. This work reports a highly sensitive and selective, room-temperature ammonia sensor with fast response/recovery based on polyaniline (PANI)/laser-induced graphene (LIG) composite fabricated by one-step laser direct writing with spraying. The sensor with optimized composite ratio and operating conditions exhibits a large response of 68 % to 60 ppm ammonia with a rapid response/recovery time of 53/167 s, together with a demonstrated detection limit of 5 ppm. Together with the miniaturized data acquisition module and integrated APP, the integrated wireless ammonia detection system demonstrates the capability to accurately detect the ammonia concentration in real time for meat preservation, health protection, and smart agriculture.

{"title":"Highly sensitive and fast response/recovery ammonia sensor based on PANI/LIG at room temperature","authors":"Li Yang , Longbiao Mao , Shuaijie Du , Zihan Wang , Wenyuan Fu , Chengpeng Yao , Luxiang Xu , Hui Zhang , Huanyu Cheng","doi":"10.1016/j.snb.2025.137710","DOIUrl":"10.1016/j.snb.2025.137710","url":null,"abstract":"<div><div>Real-time and accurate detection of ammonia in the environment provides timely alarm for excessive exposure or food spoilage. However, metal oxide-based ammonia gas sensors often need to operate at high temperatures (200–400℃) and conductive polymer-based ammonia gas sensors suffer from long response/recovery time, which are challenging for real-time wearable applications. Meanwhile, three-dimensional porous laser-induced graphene (LIG) features simple preparation, high carrier mobility, good conductivity, and large specific surface area, presenting opportunities to further combine with conductive polymers for enhanced performance. This work reports a highly sensitive and selective, room-temperature ammonia sensor with fast response/recovery based on polyaniline (PANI)/laser-induced graphene (LIG) composite fabricated by one-step laser direct writing with spraying. The sensor with optimized composite ratio and operating conditions exhibits a large response of 68 % to 60 ppm ammonia with a rapid response/recovery time of 53/167 s, together with a demonstrated detection limit of 5 ppm. Together with the miniaturized data acquisition module and integrated APP, the integrated wireless ammonia detection system demonstrates the capability to accurately detect the ammonia concentration in real time for meat preservation, health protection, and smart agriculture.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"437 ","pages":"Article 137710"},"PeriodicalIF":8.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing machine vision and image processing in multicolor lateral flow assays for cutting-edge liquid biopsy applications 在多色横向流动检测中利用机器视觉和图像处理技术，实现前沿的液体活检应用

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-04 DOI: 10.1016/j.snb.2025.137729

Eleni Lamprou , Athanasios Kokkinis , Panagiota M. Kalligosfyri , Panagiotis N. Koustoumpardis , Despina P. Kalogianni

Lateral flow assays (LFAs) are important diagnostic tools with numerous applications in various scientific fields, including diagnostics, medicine, analytical chemistry, biochemistry, environmental and food science. Artificial Intelligence (AI) and Image Processing tools are the state-of-the-art technology in analytical tools, especially in Point-of-Care (POC) devices that improve the detection efficiency without the need for highly qualified personnel. In this context, we have developed novel multicolor LFAs exploiting machine vision and image analysis tools for the automated "reading" of the visual result of LFAs using beads of different colors as reporters to distinguish between multiple targets. The system consists of a multicolor test integrated with a mobile/smartphone and a web application for the automatic interpretation of the results. The use of multicolor beads, relating each color to a specific target, enhanced image analysis-based discrimination of the tests between different targets. The developed diagnostic tool has been applied to cutting-edge liquid biopsy applications which include the detection of three different microRNA molecules spiked in urine samples. The developed integrated system has been successfully applied to a series of real samples, advancing the field of LFAs diagnostics. The system showed 99.3 % accuracy, 99.1 % sensitivity and 100 % specificity.

{"title":"Harnessing machine vision and image processing in multicolor lateral flow assays for cutting-edge liquid biopsy applications","authors":"Eleni Lamprou , Athanasios Kokkinis , Panagiota M. Kalligosfyri , Panagiotis N. Koustoumpardis , Despina P. Kalogianni","doi":"10.1016/j.snb.2025.137729","DOIUrl":"10.1016/j.snb.2025.137729","url":null,"abstract":"<div><div>Lateral flow assays (LFAs) are important diagnostic tools with numerous applications in various scientific fields, including diagnostics, medicine, analytical chemistry, biochemistry, environmental and food science. Artificial Intelligence (AI) and Image Processing tools are the state-of-the-art technology in analytical tools, especially in Point-of-Care (POC) devices that improve the detection efficiency without the need for highly qualified personnel. In this context, we have developed novel multicolor LFAs exploiting machine vision and image analysis tools for the automated \"reading\" of the visual result of LFAs using beads of different colors as reporters to distinguish between multiple targets. The system consists of a multicolor test integrated with a mobile/smartphone and a web application for the automatic interpretation of the results. The use of multicolor beads, relating each color to a specific target, enhanced image analysis-based discrimination of the tests between different targets. The developed diagnostic tool has been applied to cutting-edge liquid biopsy applications which include the detection of three different microRNA molecules spiked in urine samples. The developed integrated system has been successfully applied to a series of real samples, advancing the field of LFAs diagnostics. The system showed 99.3 % accuracy, 99.1 % sensitivity and 100 % specificity.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"437 ","pages":"Article 137729"},"PeriodicalIF":8.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microwave-based label-free immunosensor to detect anti-p53 autoantibodies 基于微波的无标记免疫传感器检测抗 p53 自身抗体

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-04 DOI: 10.1016/j.snb.2025.137702

Sebastian Montoya-Villada , Jahir Orozco , Erick Reyes-Vera

Anti-p53 autoantibody concentrations in the bloodstream have been linked to early tumor formation, making this molecule a promising cancer biomarker, including early colorectal cancer (CRC) diagnosis. Herein, a microwave-based label-free biosensor was designed for the first time by microstrip transmission line technology to detect anti-p53 autoantibodies (anti-p53 Aabs). The biosensor integrates a p53 antigen-functionalized copper surface that interacts with anti-p53 Aabs from samples and utilizes a 50-Ω microstrip line with stepped impedance resonators (SIRs) optimized to resonate at 5.2 GHz to transduce the p53-anti p53 Aabs interaction. Experimental evaluations showed that the biosensor could detect binding events with a linear response from 250 up to 1250 pg/mL, a sensitivity of 0.104 MHz*mL/pg, and a limit of detection (LOD) of 316.5 pg/mL. Leveraging the inherent advantages of microstrip-based biosensors of high sensitivity, miniaturization, and real-time monitoring could provide a transformative approach to non-invasive and label-free detection of not only anti-p53 Aabs but other cancer-related biomarkers, helping fill a significant gap in current biosensor technology and addressing an unmet need in oncology diagnostics.

{"title":"Microwave-based label-free immunosensor to detect anti-p53 autoantibodies","authors":"Sebastian Montoya-Villada , Jahir Orozco , Erick Reyes-Vera","doi":"10.1016/j.snb.2025.137702","DOIUrl":"10.1016/j.snb.2025.137702","url":null,"abstract":"<div><div>Anti-p53 autoantibody concentrations in the bloodstream have been linked to early tumor formation, making this molecule a promising cancer biomarker, including early colorectal cancer (CRC) diagnosis. Herein, a microwave-based label-free biosensor was designed for the first time by microstrip transmission line technology to detect anti-p53 autoantibodies (anti-p53 Aabs). The biosensor integrates a p53 antigen-functionalized copper surface that interacts with anti-p53 Aabs from samples and utilizes a 50-Ω microstrip line with stepped impedance resonators (SIRs) optimized to resonate at 5.2 GHz to transduce the p53-anti p53 Aabs interaction. Experimental evaluations showed that the biosensor could detect binding events with a linear response from 250 up to 1250 pg/mL, a sensitivity of 0.104 MHz*mL/pg, and a limit of detection (LOD) of 316.5 pg/mL. Leveraging the inherent advantages of microstrip-based biosensors of high sensitivity, miniaturization, and real-time monitoring could provide a transformative approach to non-invasive and label-free detection of not only anti-p53 Aabs but other cancer-related biomarkers, helping fill a significant gap in current biosensor technology and addressing an unmet need in oncology diagnostics.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"437 ","pages":"Article 137702"},"PeriodicalIF":8.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A water-stable phosphorescent probe for doxorubicin detection by phosphorescence resonance energy transfer 通过磷光共振能量转移检测多柔比星的水稳定磷光探针

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-04 DOI: 10.1016/j.snb.2025.137739

Da Jun Wu , Li Ya Liang , Yichen Shi , Ya Ting Gao , Shuai Chang , Jian Lv , Ruo Can Qian , Da Wei Li , Bin Bin Chen

Phosphorescent sensing probes have attracted considerable attention due to their ability to eliminate autofluorescence interference in biological samples. However, constructing water-stable phosphorescent materials remains a significant challenge because of water-quenched phosphorescence (phos.) property. Herein, a facile one-step thermal polymerization is proposed to synthesize aluminium/4-chlorobenzoicacid phosphorescent materials (Al/PCBA-PMs). The obtained Al/PCBA-PMs have two phos. emissions at 438 nm and 515 nm, respectively, with a lifetime of 83.67 ms. Importantly, the Al/PCBA-PMs show good water-stable phosphorescent property, and the introduction of water can cause a change in phosphorescent color of Al/PCBA-PMs from blue to green, but which has only a small impact on phosphorescent intensity and lifetime. Furthermore, a system of phos. resonance energy transfer (PhRET) can be constructed where Al/PCBA-PMs act as phosphorescent donors and doxorubicin (DOX) serves as fluorescent acceptors. This PhRET system has a high efficiency of 44.25 %, which can achieve the direct and precise determination of DOX in biological samples with strong autofluorescence, with a linear range of 0 −100 μM. This work proposes a powerful strategy for constructing water-stable phosphorescent probes and develops a new type of PhRET system to achieve precise phosphorescent detection in biological samples.

{"title":"A water-stable phosphorescent probe for doxorubicin detection by phosphorescence resonance energy transfer","authors":"Da Jun Wu , Li Ya Liang , Yichen Shi , Ya Ting Gao , Shuai Chang , Jian Lv , Ruo Can Qian , Da Wei Li , Bin Bin Chen","doi":"10.1016/j.snb.2025.137739","DOIUrl":"10.1016/j.snb.2025.137739","url":null,"abstract":"<div><div>Phosphorescent sensing probes have attracted considerable attention due to their ability to eliminate autofluorescence interference in biological samples. However, constructing water-stable phosphorescent materials remains a significant challenge because of water-quenched phosphorescence (phos.) property. Herein, a facile one-step thermal polymerization is proposed to synthesize aluminium/4-chlorobenzoicacid phosphorescent materials (Al/PCBA-PMs). The obtained Al/PCBA-PMs have two phos. emissions at 438 nm and 515 nm, respectively, with a lifetime of 83.67 ms. Importantly, the Al/PCBA-PMs show good water-stable phosphorescent property, and the introduction of water can cause a change in phosphorescent color of Al/PCBA-PMs from blue to green, but which has only a small impact on phosphorescent intensity and lifetime. Furthermore, a system of phos. resonance energy transfer (PhRET) can be constructed where Al/PCBA-PMs act as phosphorescent donors and doxorubicin (DOX) serves as fluorescent acceptors. This PhRET system has a high efficiency of 44.25 %, which can achieve the direct and precise determination of DOX in biological samples with strong autofluorescence, with a linear range of 0 −100 μM. This work proposes a powerful strategy for constructing water-stable phosphorescent probes and develops a new type of PhRET system to achieve precise phosphorescent detection in biological samples.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"437 ","pages":"Article 137739"},"PeriodicalIF":8.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Target-triggered core-satellite assemblies with fluorescence-SERS cascade amplification for intracellular microRNA detection 利用荧光-SERS 级联放大技术进行细胞内 microRNA 检测的目标触发核心-卫星组件

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-04 DOI: 10.1016/j.snb.2025.137759

Conghui Liu, Juejiao Huang, Ruixiang Liu, Yan Zhu, Tailin Xu

Controllable DNA-assembled plasmonic nanosensing platforms represent a promising approach for intracellular nucleic acid detection based on the significant optical signal changes induced by target recognition. In this work, we engineered a target-triggered self-assembly strategy for intracellular microRNA-21 (miRNA-21) detection with fluorescence-SERS cascade amplification. This strategy employs telomerase (TE) as the catalytic driver, gold nanoparticles and gold nanorods as the assembled components, and a strand displacement amplification (SDA) reaction as the linking tool. In the presence of both miRNA-21 and TE, the platform undergoes target-triggered core-satellite assemblies, enabling fluorescence recovery and SERS signal enhancement via plasmonic coupling-induced "hot spots". By this sensing strategy, the target miRNA-21 can be detected in a wide linear range (10 fM to 100 pM) with a limit of detection of 0.165 fM in the SERS mode and 0.196 fM in the fluorescence mode. This highly specific dual-signal response minimizes false positives, addressing cancer biomarker heterogeneity and improving the reliability of early cancer diagnosis. By integrating sensitivity, specificity, and a multi-dimensional optical readout, this strategy represents a potential tool in live-cell sensing and biomarker-driven cancer diagnostics.

{"title":"Target-triggered core-satellite assemblies with fluorescence-SERS cascade amplification for intracellular microRNA detection","authors":"Conghui Liu, Juejiao Huang, Ruixiang Liu, Yan Zhu, Tailin Xu","doi":"10.1016/j.snb.2025.137759","DOIUrl":"10.1016/j.snb.2025.137759","url":null,"abstract":"<div><div>Controllable DNA-assembled plasmonic nanosensing platforms represent a promising approach for intracellular nucleic acid detection based on the significant optical signal changes induced by target recognition. In this work, we engineered a target-triggered self-assembly strategy for intracellular microRNA-21 (miRNA-21) detection with fluorescence-SERS cascade amplification. This strategy employs telomerase (TE) as the catalytic driver, gold nanoparticles and gold nanorods as the assembled components, and a strand displacement amplification (SDA) reaction as the linking tool. In the presence of both miRNA-21 and TE, the platform undergoes target-triggered core-satellite assemblies, enabling fluorescence recovery and SERS signal enhancement via plasmonic coupling-induced \"hot spots\". By this sensing strategy, the target miRNA-21 can be detected in a wide linear range (10 fM to 100 pM) with a limit of detection of 0.165 fM in the SERS mode and 0.196 fM in the fluorescence mode. This highly specific dual-signal response minimizes false positives, addressing cancer biomarker heterogeneity and improving the reliability of early cancer diagnosis. By integrating sensitivity, specificity, and a multi-dimensional optical readout, this strategy represents a potential tool in live-cell sensing and biomarker-driven cancer diagnostics.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"437 ","pages":"Article 137759"},"PeriodicalIF":8.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TEA gas sensor based on MoV2O8 nanorods for seafood spoilage detection

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-03 DOI: 10.1016/j.snb.2025.137752

Yao Wang, Zhihua Zhao

Amine gases have become essential indicators for assessing seafood spoilage, with triethylamine (TEA) standing out due to its close association with the decay process. Effective detection of TEA in complex environments is therefore crucial. However, current TEA sensors often suffer from limitations such as low sensitivity, poor selectivity, or the need for high operating temperatures. In this work, a highly efficient TEA gas sensor which based on MoV₂O₈ was successfully fabricated through hydrothermal reaction and assisted calcination. Gas sensing tests demonstrated a response of 2.63 towards 100 ppm TEA at the optimal working temperature of 160℃, exhibited good linearity at low TEA concentrations. Additionally, experimental results confirmed that the sensor possesses excellent selectivity, reproducibility, and stability. The gas sensing mechanism was further analyzed in depth. Finally, a TEA detection device based on MoV₂O₈ was designed to detect TEA emissions from decayed marine fish.

引用次数: 0

Rapid detection of Staphylococcus aureus using a CRISPR/Cas14a-assisted electrochemical aptasensor and PCN-222@AuPt nanozyme-induced amplification strategy

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-03 DOI: 10.1016/j.snb.2025.137751

Yuanyuan Hui , Xiaoxia Chen , Lusha Wei , Ding Yang , Meixue Pu , Yazhou Mao , Zhanmin Wang , Bini Wang

The rapid determination of Staphylococcus aureus (S.auerus) is essential for advancing food safety monitoring and control. In this study, we developed a novel electrochemical aptasensor leveraging the trans-cleavage activity of CRISPR/Cas14a, augmented by nanomaterial modification and nanozyme-catalyzed signal amplification, to enable ultrasensitive and selective detection of S. aureus. The glassy carbon electrode (GCE) was modified with chromium-based metal–organic framework/polypyrrole/gold nanoparticle composites (Cr-MOF/PPy@Au), significantly improving electrical conductivity and biocompatibility. The aptamer-mediated specific recognition of S. aureus was coupled with CRISPR/Cas14a trans-cleavage activity, ensuring high detection specificity. Simultaneously, the peroxidase (POD)-like catalytic activity of the zirconium-porphyrin metal-organic framework hybridized with AuPt nanoparticles (PCN-222@AuPt nanozyme) facilitated the efficient conversion of hydrogen peroxide into hydroxyl radicals, thereby amplifying the electrochemical signal. Under optimized experimental parameters, the proposed sensor achieved a broad linear detection range from 5 × 10 ¹ to 5 × 10⁷ CFU mL⁻¹ and a low detection limit of 10 CFU mL⁻¹ . This study work presents a reproducible and highly selective platform for S. aureus detection, highlighting its potential for future applications in food safety monitoring.

{"title":"Rapid detection of Staphylococcus aureus using a CRISPR/Cas14a-assisted electrochemical aptasensor and PCN-222@AuPt nanozyme-induced amplification strategy","authors":"Yuanyuan Hui , Xiaoxia Chen , Lusha Wei , Ding Yang , Meixue Pu , Yazhou Mao , Zhanmin Wang , Bini Wang","doi":"10.1016/j.snb.2025.137751","DOIUrl":"10.1016/j.snb.2025.137751","url":null,"abstract":"<div><div>The rapid determination of <em>Staphylococcus aureus</em> (<em>S.auerus</em>) is essential for advancing food safety monitoring and control. In this study, we developed a novel electrochemical aptasensor leveraging the trans-cleavage activity of CRISPR/Cas14a, augmented by nanomaterial modification and nanozyme-catalyzed signal amplification, to enable ultrasensitive and selective detection of <em>S. aureus</em>. The glassy carbon electrode (GCE) was modified with chromium-based metal–organic framework/polypyrrole/gold nanoparticle composites (Cr-MOF/PPy@Au), significantly improving electrical conductivity and biocompatibility. The aptamer-mediated specific recognition of <em>S. aureus</em> was coupled with CRISPR/Cas14a trans-cleavage activity, ensuring high detection specificity. Simultaneously, the peroxidase (POD)-like catalytic activity of the zirconium-porphyrin metal-organic framework hybridized with AuPt nanoparticles (PCN-222@AuPt nanozyme) facilitated the efficient conversion of hydrogen peroxide into hydroxyl radicals, thereby amplifying the electrochemical signal. Under optimized experimental parameters, the proposed sensor achieved a broad linear detection range from 5 × 10 ¹ to 5 × 10⁷ CFU mL⁻¹ and a low detection limit of 10 CFU mL⁻¹ . This study work presents a reproducible and highly selective platform for <em>S. aureus</em> detection, highlighting its potential for future applications in food safety monitoring.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"437 ","pages":"Article 137751"},"PeriodicalIF":8.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced low-concentration CO2 sensing performance for Sr- and Co-doped LaFeO3 thick film sensors by oxygen-enriched atmosphere after vacuum annealing 真空退火后在富氧环境下提高掺杂硒和钴的氧化钴厚膜传感器的低浓度二氧化碳传感性能

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-03 DOI: 10.1016/j.snb.2025.137736

Xiaohong Su, Haotian Shan, Yanting Tian, Wei Guo, Peng Zhao, Lin Xue, Yongjia Zhang

This study investigates the enhancement of low-concentration CO₂ sensing performance in Sr- and Co-doped LaFeO₃ (LFO) thick film sensors through a combined treatment involving vacuum annealing and oxygen-rich atmosphere exposure. La_0.8Sr_0.2FeO₃ (LSFO) and LaFe_0.8Co_0.2O₃ (LFCO) porous microspheres were synthesized via hydrothermal method and characterized using XRD, SEM, XPS, and Raman spectroscopy. The optimized LFCO sensor demonstrated a high response value of 1.158 at 110°C, with rapid response/recovery times and excellent moisture resistance. The improved sensitivity was attributed to increased surface oxygen vacancies from vacuum annealing, promoting oxygen adsorption and electron transfer with CO₂. First-principles calculations confirmed the role of O₂ adsorption in enhancing the number of transferred electrons for CO₂ molecules, thus improving gas sensing performance. This study presents a practical approach for developing cost-effective, high-performance CO₂ sensors with enhanced sensitivity and stability.

{"title":"Enhanced low-concentration CO2 sensing performance for Sr- and Co-doped LaFeO3 thick film sensors by oxygen-enriched atmosphere after vacuum annealing","authors":"Xiaohong Su, Haotian Shan, Yanting Tian, Wei Guo, Peng Zhao, Lin Xue, Yongjia Zhang","doi":"10.1016/j.snb.2025.137736","DOIUrl":"10.1016/j.snb.2025.137736","url":null,"abstract":"<div><div>This study investigates the enhancement of low-concentration CO<sub>2</sub> sensing performance in Sr- and Co-doped LaFeO<sub>3</sub> (LFO) thick film sensors through a combined treatment involving vacuum annealing and oxygen-rich atmosphere exposure. La<sub>0.8</sub>Sr<sub>0.2</sub>FeO<sub>3</sub> (LSFO) and LaFe<sub>0.8</sub>Co<sub>0.2</sub>O<sub>3</sub> (LFCO) porous microspheres were synthesized via hydrothermal method and characterized using XRD, SEM, XPS, and Raman spectroscopy. The optimized LFCO sensor demonstrated a high response value of 1.158 at 110°C, with rapid response/recovery times and excellent moisture resistance. The improved sensitivity was attributed to increased surface oxygen vacancies from vacuum annealing, promoting oxygen adsorption and electron transfer with CO<sub>2</sub>. First-principles calculations confirmed the role of O<sub>2</sub> adsorption in enhancing the number of transferred electrons for CO<sub>2</sub> molecules, thus improving gas sensing performance. This study presents a practical approach for developing cost-effective, high-performance CO<sub>2</sub> sensors with enhanced sensitivity and stability.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"437 ","pages":"Article 137736"},"PeriodicalIF":8.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143766373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas12a-calibrated biosensor for accurate detection of low-frequency single nucleotide variants via loop-assisted adjustable entropy-driven catalytic reaction

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-03 DOI: 10.1016/j.snb.2025.137755

Fang Zhu , Zinian Cai , Jiahui Dai , Fengxiang Ai , Yibin Deng , Beibei Wu , Zhen Zhang

Accurate determination of single nucleotide variants (SNVs) with low variant-allele frequencies (VAFs) in clinical samples is urgently needed but challenging, since subtle Gibbs free energy differences of mutation-type (MT) sequences cannot overwhelm the interferences caused by high concentrations of wild-type (WT) sequences. Herein, a novel biosensor was constructed for accurately identifying SNVs with low VAFs (< 0.1 %) by a loop-assisted adjustable entropy-driven catalytic reaction (EDR), benefiting from the adjusted Gibbs free energy for these interferences elimination. At the detection system, the binding energy between WT sequences and EDR induced is restricted through the insertion of a loop structure in the toehold region, reducing the interferences generated by high concentrations of WT sequences. Furthermore, the CRISPR/Cas12a system functions as a synergistic signal calibrator, thereby markedly enhancing the sensitivity and specificity of the EDR through its precise RNA-guided nucleic acid recognition and signal amplification capability. Moreover, our proposed method was applied for drug-resistant mutations of Mycobacterium tuberculosis analysis from synthetic DNA and clinical samples, and excellent accuracy indicated its great potential as next-generation sequencing (NGS). This work provides some new insights about highly specific bioassays construction in disease diagnosis.

{"title":"CRISPR/Cas12a-calibrated biosensor for accurate detection of low-frequency single nucleotide variants via loop-assisted adjustable entropy-driven catalytic reaction","authors":"Fang Zhu , Zinian Cai , Jiahui Dai , Fengxiang Ai , Yibin Deng , Beibei Wu , Zhen Zhang","doi":"10.1016/j.snb.2025.137755","DOIUrl":"10.1016/j.snb.2025.137755","url":null,"abstract":"<div><div>Accurate determination of single nucleotide variants (SNVs) with low variant-allele frequencies (VAFs) in clinical samples is urgently needed but challenging, since subtle Gibbs free energy differences of mutation-type (MT) sequences cannot overwhelm the interferences caused by high concentrations of wild-type (WT) sequences. Herein, a novel biosensor was constructed for accurately identifying SNVs with low VAFs (< 0.1 %) by a loop-assisted adjustable entropy-driven catalytic reaction (EDR), benefiting from the adjusted Gibbs free energy for these interferences elimination. At the detection system, the binding energy between WT sequences and EDR induced is restricted through the insertion of a loop structure in the toehold region, reducing the interferences generated by high concentrations of WT sequences. Furthermore, the CRISPR/Cas12a system functions as a synergistic signal calibrator, thereby markedly enhancing the sensitivity and specificity of the EDR through its precise RNA-guided nucleic acid recognition and signal amplification capability. Moreover, our proposed method was applied for drug-resistant mutations of <em>Mycobacterium tuberculosis</em> analysis from synthetic DNA and clinical samples, and excellent accuracy indicated its great potential as next-generation sequencing (NGS). This work provides some new insights about highly specific bioassays construction in disease diagnosis.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"437 ","pages":"Article 137755"},"PeriodicalIF":8.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143775827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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