Sensors and Actuators B: Chemical最新文献_第2页

Photocurrent-polarity-switching photoelectrochemical and electrochemical dual-mode immunosensor for staphylococcal enterotoxin B assay based on multifunctional COF@hemin probe 基于多功能COF@hemin探针的葡萄球菌肠毒素B检测的光电-极性开关光电-电化学双模免疫传感器

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-05-15 Epub Date: 2026-02-09 DOI: 10.1016/j.snb.2026.139636

Guizhen Luo , Ke Xiao , Cuicui Du , Xiaohua Zhang , Jinhua Chen

Staphylococcus enterotoxin B (SEB), a superantigen secreted by Staphylococcus aureus, is a significant pathogenic factor responsible for foodborne infections and food poisoning. Therefore, the sensitive detection of SEB is crucial and remains challenging. Herein, a dual-mode immunosensor that integrated photoelectrochemical (PEC) and electrochemical (EC) techniques was developed for the highly sensitive analysis of SEB, utilizing multifunctional hemin-loaded covalent organic frameworks (COF@hemin) as a signaling probe capable of both switching photocurrent polarity and generating electrochemical signals. The COF@hemin composite was synthesized by functionalization of COF-V with sulfhydryl groups, enabling efficient immobilization of hemin via a sulfhydryl-ene click reaction. After that, SEB antibody (Ab2) was connected to the COF@hemin composite through amide bonding to form Ab2-COF@hemin. Based on the SEB-mediated specific immune sandwich reaction, Ab2-COF@hemin was captured by a photoactive zinc-based metal-organic framework (Zn-MOF)-modified ITO electrode which was pre-immobilized with primary antibody (Ab1). The introduced COF@hemin not only switched the anodic photocurrent of Zn-MOF/ITO electrode to cathodic photocurrent, but also generated high electrochemical response due to the redox ability of hemin. Thus, the constructed PEC-EC dual-mode sensor enabled sensitive detection of SEB, demonstrating a linear range from 0.1 pg mL⁻¹ to 10 ng mL⁻¹ and a detection limit of 0.03 pg mL⁻¹ in PEC mode, and a linear range from 1 pg mL⁻¹ to 5 ng mL⁻¹ with a detection limit of 0.2 pg mL⁻¹ in EC mode. In addition, the proposed immunosensor demonstrated satisfactory performance in SEB detection in complex samples, indicating its potential applicability in food safety monitoring.

葡萄球菌肠毒素B （SEB）是金黄色葡萄球菌分泌的一种超抗原，是食源性感染和食物中毒的重要致病因子。因此，SEB的敏感检测至关重要，但仍然具有挑战性。本文开发了一种集成光电化学（PEC）和电化学（EC）技术的双模免疫传感器，用于高灵敏度分析SEB，利用多功能血红素负载共价有机框架（COF@hemin）作为能够切换光电流极性和产生电化学信号的信号探针。通过巯基功能化COF-V合成了COF@hemin复合材料，通过巯基-烯咔嗒反应实现了hemin的高效固定化。然后将SEB抗体（Ab2）通过酰胺键与COF@hemin复合物连接，形成Ab2-COF@hemin。基于seb介导的特异性免疫夹心反应，采用一抗（Ab1）预固定的光活性锌基金属有机框架（Zn-MOF）修饰ITO电极捕获Ab2-COF@hemin。所引入的COF@hemin不仅将Zn-MOF/ITO电极的阳极光电流转换为阴极光电流，而且由于hemin的氧化还原能力，产生了较高的电化学响应。因此，构建的PEC-EC双模传感器实现了对SEB的灵敏检测，在PEC模式下线性范围为0.1 pg mL-1至10 ng mL-1，检测限为0.03 pg mL-1，在EC模式下线性范围为1 pg mL-1至5 ng mL-1，检测限为0.2 pg mL-1。此外，该免疫传感器在复杂样品的SEB检测中表现出令人满意的性能，表明其在食品安全监测中的潜在适用性。

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引用次数: 0

A high-sensitivity aptamer-phage dual-recognition strategy for electrochemical detection of Salmonella typhimurium in poultry 电化学检测家禽鼠伤寒沙门菌的高灵敏度适配体-噬菌体双识别策略

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-05-15 Epub Date: 2026-02-08 DOI: 10.1016/j.snb.2026.139632

Yihang Yang , Xinyu Fang , Cong Liu , Shulei Zhang , Zhiyu Yang , Xu Jin , Liling Hao , Fei Xu

Salmonella typhimurium (S. typhimurium) is a prominent foodborne pathogen frequently detected in poultry and egg products, posing persistent challenges to food safety. Herein, we present a dual-recognition electrochemical biosensor that integrates aptamers and bacteriophages (phages) for highly sensitive detection of S. typhimurium. Tetrahedral DNA nanostructures (TDNs) incorporating S. typhimurium aptamers were employed to spatially assemble aptamers on the electrode surface, thereby improving capture efficiency. Meanwhile, phages were directionally immobilized on calcined zeolitic imidazolate framework (QZIF-67) nanostructures loaded with methylene blue (MB) via electrostatic adsorption, serving as both biorecognition and signal amplification elements. Upon target recognition, an “aptamer-bacterium-phage” sandwich complex was formed, which brought MB molecules closer to the electrode and significantly enhanced the electrochemical response. The biosensor achieved an ultralow detection limit of 2.6 CFU/mL and demonstrated excellent recovery rates (90.09–108.70 %) in spiked chicken and egg samples, confirming its reliability and applicability. This work provides a practical and integrated approach for rapid and ultrasensitive bacteria detection in food safety monitoring.

鼠伤寒沙门氏菌（S. typhimurium）是一种重要的食源性病原体，经常在家禽和蛋类产品中检测到，对食品安全构成了持续的挑战。在此，我们提出了一种双识别电化学生物传感器，该传感器集成了适体和噬菌体，用于高灵敏度检测鼠伤寒沙门氏菌。采用含有鼠伤寒沙门氏菌适配体的四面体DNA纳米结构（TDNs）在电极表面对适配体进行空间组装，从而提高了捕获效率。同时，通过静电吸附将噬菌体定向固定在负载亚甲基蓝（MB）的煅烧沸石咪唑酸骨架（QZIF-67）纳米结构上，作为生物识别元件和信号放大元件。靶识别后，形成“适体-细菌-噬菌体”夹心复合物，使MB分子更靠近电极，显著增强了电化学响应。该传感器在加标鸡肉和鸡蛋样品中的检出限为2.6 CFU/mL，回收率为90.09 ~ 108.70%，验证了该传感器的可靠性和适用性。本研究为食品安全监测中快速、超灵敏的细菌检测提供了一种实用的综合方法。

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引用次数: 0

A rapid, sample-to-answer strategy for the multiplex detection of intact respiratory pathogens 一种快速、从样本到答案的策略，用于完整呼吸道病原体的多重检测

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-05-15 Epub Date: 2026-02-12 DOI: 10.1016/j.snb.2026.139658

Shijue Gao , Liang Bai , Yujie Xiang , Hong Liu , Zifan Yang , Fengyu Tian , Xiaoping Chen , Xinxin Shen , Xuejun Ma

Respiratory pathogens have long represented a significant cause of global pandemics, highlighting the critical need for reliable tools to evaluate their infectivity. However, conventional nucleic acid- or antigen-based detection methods often fail to accurately reflect the actual infection risk posed by the intact pathogens present in clinical samples. To address this limitation, this study developed a detection strategy for six-plex intact respiratory pathogens in swabs, coupling a broad-spectrum capture and viability dye treatment with an automated microfluidic platform. Specifically, recombinant human mannan-binding lectin-functionalized magnetic beads (rhMBL-MBs) are used to capture intact pathogens, while improved propidium monoazide (PMAxx) prevents amplification of compromised ones. Following this pretreatment, the microfluidic platform automates the processes of nucleic acid extraction, recombinase aided amplification (RAA), fluorescence detection, and wireless data transmission. This sample-to-answer workflow delivers results within 45 min, achieving the lowest detection limits of 5 50 % tissue culture infectious dose per milliliter (TCID₅₀/mL) for intact pathogens. The clinical utility of this strategy was validated using 40 swabs collected at multiple time points post-diagnosis (confirmed by RT-qPCR/qPCR) and 10 healthy controls. Results showed a 100 % detection rate for samples collected 2–3 days post-diagnosis and 40 % for those collected 7–10 days post-diagnosis, demonstrating its effective differentiation of intact, potentially infectious pathogens in clinical specimens. With high specificity, rapid turnaround, and design flexibility, this approach shows strong potential for assessing transmission risks and informing evidence-based infection control strategies, including decisions on isolation duration.

呼吸道病原体长期以来一直是全球流行病的一个重要原因，因此迫切需要可靠的工具来评估其传染性。然而，传统的基于核酸或抗原的检测方法往往不能准确反映临床样本中完整病原体所带来的实际感染风险。为了解决这一限制，本研究开发了一种针对拭子中六重完整呼吸道病原体的检测策略，将广谱捕获和活力染料处理与自动化微流控平台相结合。具体来说，重组人甘露聚糖结合凝集素功能化磁珠（rhmbl - mb）用于捕获完整的病原体，而改进的单叠氮丙啶（PMAxx）可防止受损病原体的扩增。预处理后，微流控平台自动完成核酸提取、重组酶辅助扩增（RAA）、荧光检测和无线数据传输等过程。这种从样品到答案的工作流程在45 min内提供结果，对于完整的病原体，达到最低检测限为5 50 %组织培养感染剂量每毫升（TCID50/mL）。通过在诊断后多个时间点收集的40份拭子（经RT-qPCR/qPCR证实）和10名健康对照，验证了该策略的临床实用性。结果显示，诊断后2-3天的标本检出率为100 %，7-10天的标本检出率为40 %，表明该方法可有效鉴别临床标本中完整的、具有潜在传染性的病原体。该方法具有高特异性、快速周转和设计灵活性，在评估传播风险和告知基于证据的感染控制策略（包括决定隔离时间）方面显示出强大的潜力。

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引用次数: 0

Highly sensitive and selective determination of nuclear matrix protein 22 based on an epitope imprinted electrochemical biosensor 基于表位印迹电化学生物传感器的核基质蛋白22的高灵敏度和选择性测定

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-05-15 Epub Date: 2026-02-07 DOI: 10.1016/j.snb.2026.139623

Xia He , Yuankun Zheng , Yuting Zhang , Jia He , Mengting Li , Jinqi Li , Zhenping Liu , Huikai Shao

Nuclear matrix protein 22 (NMP22) is a well-recognized biomarker for bladder cancer; however, conventional detection methods often suffer from insufficient sensitivity, high-cost and time-consuming. In this work, a novel epitope molecularly imprinted polymer (EMIP)-based electrochemical biosensor was constructed for highly selective and sensitive determination of NMP22. The EMIP biosensor was fabricated by electro-polymerization with C-terminal dodecapeptide (GATPRAKGKAKH, named as epitope) of NMP22 as template and o-phenylenediamine (o-PD) as functional monomer on the gold electrode (AuE) which was previously self-assembled with thiolated graphene (G-SH) and gold nanoparticles (AuNPs). The use of G-SH and AuNPs can supply synergistic effects for enhancing detection sensitivity and epitope imprinting of C-terminal dodecapeptide of NMP22 can provide high selectivity for NMP22 detection. To obtain the superior sensitivity, several experimental parameters affecting the performance of the EMIP electrochemical sensor were investigated, including the molar ratio of template to functional monomer, electropolymerization cycle, elution time and incubation time. Under the optimal experiment, this EMIP electrochemical biosensor exhibited a good linear relationship for NMP22 in the concentration range from 0.1 pg mL⁻¹ to 1.0 μg mL⁻¹, with a limit of detection (LOD) of 0.077 pg mL⁻¹. Finally, the EMIP electrochemical biosensor was successfully used for the determination of NMP22 in human urine with the recovery rates ranging from 96.92 % to 100.88 %. Compared with conventional ELISA, the proposed EMIP biosensor displayed shorter detection time, improved reproducibility and comparable analytical reliability. These findings highlight that the developed EMIP biosensor holds strong potentials for early bladder cancer diagnosis and broader clinical applications.

核基质蛋白22 （NMP22）是膀胱癌公认的生物标志物；然而，传统的检测方法往往存在灵敏度不足、成本高、耗时长的问题。本研究构建了一种基于表位分子印迹聚合物（EMIP）的电化学生物传感器，用于NMP22的高选择性和高灵敏度检测。EMIP生物传感器以NMP22的c端十二肽（GATPRAKGKAKH，命名为表位）为模板，邻苯二胺（o-PD）为功能单体，通过电聚合制备在金电极（AuE）上，该电极由硫代石墨烯（G-SH）和金纳米颗粒（AuNPs）自组装而成。G-SH和AuNPs的协同作用可提高检测灵敏度，而NMP22 c端十二肽表位印迹可为NMP22的检测提供高选择性。为了获得优异的灵敏度，研究了影响EMIP电化学传感器性能的几个实验参数，包括模板与功能单体的摩尔比、电聚合周期、洗脱时间和孵育时间。在最佳实验条件下，EMIP电化学生物传感器对NMP22在0.1 pg mL−1 ~ 1.0 μg mL−1范围内具有良好的线性关系，检出限（LOD）为0.077 pg mL−1。最后，EMIP电化学生物传感器成功地用于人尿中NMP22的测定，回收率为96.92 % ~ 100.88 %。与传统ELISA相比，EMIP生物传感器具有检测时间短、重现性好、分析可靠性高的特点。这些发现表明，EMIP生物传感器在膀胱癌早期诊断和更广泛的临床应用方面具有很强的潜力。

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引用次数: 0

A DNA logic gate-regulated ECL biosensor for detecting cfDNA from multidrug-resistant Mycobacterium tuberculosis 用于检测耐多药结核分枝杆菌cfDNA的DNA逻辑门调控ECL生物传感器

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-05-15 Epub Date: 2026-02-12 DOI: 10.1016/j.snb.2026.139655

Shihua Sun , Wenyan Li , Weiyao Zhong , Fangyan Ji , Peilin Wang , Jingwei Shi , Qiang Ma

Multidrug-resistant tuberculosis (MDR-TB) is a form of tuberculosis caused by Mycobacterium tuberculosis strains that are resistant to at least rifampicin (RIF) and isoniazid (INH) simultaneously. The most common mutations causing RIF and INH resistance are rpoB531 and katG315, respectively. This study innovatively develops a DNA logic gate-regulated electrochemiluminescence–resonance energy transfer (ECL-RET) sensor to achieve specific identification of cell-free DNA (cfDNA) from drug-resistant Mycobacterium tuberculosis. Zn-metal organic framework (Zn-MOF) with excellent anodic ECL performance is prepared as the ECL emitter and energy donor. Meanwhile, Au NPs-MXene heterojunction is synthesized as the ECL-RET acceptor. In this DNA logic gate-regulated sensing system, two cfDNA strands with rpoB531 and katG315 mutations function as inputs for an AND logic gate, which further activates toehold-mediated strand displacement (TMSD) to release the connecting chain. As a result, the ECL-RET between Zn-MOF and Au NPs-MXene heterojunction is achieved. This DNA logic gate-regulated ECL sensor is used to detect rpoB531 and katG315 mutation cfDNA in urine samples successfully. The sensitive detection for rpoB531 and katG315 is achieved with a linear range of 10 fM to 100 nM and a limit of detection (LOD) of 0.9 fM. This strategy offers a novel method for the non-invasive and rapid diagnosis of MDR-TB.

耐多药结核病（MDR-TB）是由结核分枝杆菌菌株引起的一种结核病，至少同时对利福平（RIF）和异烟肼（INH）具有耐药性。引起RIF和INH耐药的最常见突变分别是rpoB531和katG315。本研究创新性地开发了一种DNA逻辑门调控的电化学发光共振能量转移（ECL-RET）传感器，以实现耐药结核分枝杆菌无细胞DNA （cfDNA）的特异性鉴定。制备了具有优异阳极ECL性能的锌金属有机骨架（Zn-MOF）作为ECL发射极和能量供体。同时，合成了Au NPs-MXene异质结作为ECL-RET受体。在这个DNA逻辑门调控的传感系统中，两条具有rpoB531和katG315突变的cfDNA链作为and逻辑门的输入，进一步激活脚位介导的链位移（TMSD）以释放连接链。实现了Zn-MOF和Au NPs-MXene异质结之间的ECL-RET。该DNA逻辑门调控ECL传感器成功地检测了尿样中rpoB531和katG315突变cfDNA。rpoB531和katG315的灵敏度检测范围为10 fM ~ 100 nM，检测限（LOD）为0.9 fM。这一策略为耐多药结核病的非侵入性快速诊断提供了一种新方法。

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引用次数: 0

Quasi-bound states in the continuum empowered terahertz metamaterial sensor for imidacloprid detection in fruits 连续介质准束缚态授权太赫兹超材料传感器用于水果中吡虫啉的检测

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-05-15 Epub Date: 2026-02-08 DOI: 10.1016/j.snb.2026.139633

Lixin Ma , Xiaonan Yang , Chen Wang , Ruiyun Zhou , Zhikun Yang , Li Sun , Zhiming Guo , Jianrong Cai , Xiaobo Zou

Imidacloprid, a neonicotinoid pesticide commonly found in fruits and vegetables, poses a health hazard when present in excessive residues. This paper designs a terahertz metamaterial sensor based on quasi-bound state in the continuum (QBIC) resonances, achieved by a structure consisting of an external square open-ring and an internal dual-rod structure. Compared with individual components, the coupled meta-atomic array formed by the dual-rod and the open-ring has a stronger electric field intensity. Besides, the effective sensing area of this coupled structure is more than 2.5 times that of the two individual components combined, demonstrating a synergistic “1 + 1 > 2” effect. By adjusting the rod lengths of the dual-rod, a metamaterial exhibiting QBIC resonance at 0.89 THz was obtained, aligning with the absorption peak of imidacloprid and thereby enhancing detection sensitivity. Experimental results show that the QBIC metamaterial sensor exhibits excellent performance, with a linear range of 0.1 mg/kg–1000 mg/kg and a calculated limit of detection of 0.072 mg/kg. Additionally, in spiked orange samples, recovery rates ranged from 92.2 % to 104.19 %, with no significant difference from high-performance liquid chromatography results. These findings provide a novel strategy for sensitive terahertz detection of imidacloprid pesticide in real samples.

吡虫啉是一种常见于水果和蔬菜中的新烟碱类杀虫剂，如果过量残留，会对健康造成危害。本文设计了一种基于准束缚态连续共振（QBIC）的太赫兹超材料传感器，该传感器由外部方形开环和内部双棒结构组成。与单个元件相比，双棒和开环形成的耦合元原子阵列具有更强的电场强度。此外，该耦合结构的有效传感面积是两个单独元件组合的2.5倍以上，呈现出“1 + 1 > 2”的协同效应。通过调整双棒的杆长，获得了在0.89 THz处表现出QBIC共振的超材料，与吡虫啉的吸收峰对齐，从而提高了检测灵敏度。实验结果表明，QBIC超材料传感器具有良好的性能，线性范围为0.1 mg/kg ~ 1000 mg/kg，计算检出限为0.072 mg/kg。加样回收率在92.2% ~ 104.19%之间，与高效液相色谱法无显著差异。这些发现为实际样品中吡虫啉农药的太赫兹灵敏检测提供了一种新的策略。

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引用次数: 0

Deep learning enabled magnetic/rare earth hybrid nanorobots for multi-modal bioimaging and temperature sensing with surgical boundary determination 基于深度学习的磁性/稀土混合纳米机器人，可用于多模态生物成像和具有手术边界确定的温度传感

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-05-15 Epub Date: 2026-02-14 DOI: 10.1016/j.snb.2026.139673

Wenjing Li , Bi Lin , Bixiao Li , Peifu Zhang , Ziyue Ju , Anees A. Ansari , Ruichan Lv

Nanorobots, with miniature size, wireless operation, and flexible mobility, can access narrow spaces and are widely used in biomedicine. High-precision transport is vital for targeted drug delivery, disease diagnosis, and treatment, yet current research has limitations like insufficient single-imaging diagnosis and active tumor targeting. In this research, a magnetic nanorobot platform is proposed, combining magnetic ferric oxide substrate with rare earth nanoparticles (RENPs). This magnetic nanorobot features multi-modal imaging including NIR II fluorescence, MRI, and PA with high moving target effect (<5 s). After conjugated with antibodies against MET and VEGF, this platform enables multi-molecular targeting. Under an external magnetic field, the dual-targeting (active and physical magnetic) allows in vitro and in vivo precise tumor cell targeting with tumor imaging. Moreover, its NIR II luminescence enables enhanced real-time temperature detection (Region: 24.6–41.5℃). Especially, the deep learning allows NIR II imaging to simultaneously perform increased fluorescence imaging for surgical boundary definition and temperature sensing display (R²= 0.973, RMSE = 1.50℃). With high penetration and spatial resolution, this proposed magnetic/rare earth hybrid nanorobots holds great promise for precision biomedicine.

纳米机器人具有体积小、无线操作、移动灵活等特点，可以进入狭窄的空间，在生物医学领域有着广泛的应用。高精度运输对于靶向药物递送、疾病诊断和治疗至关重要，但目前的研究存在单一影像诊断不足、肿瘤靶向性不强等局限性。本研究提出了一种将磁性氧化铁衬底与稀土纳米粒子（RENPs）结合的磁性纳米机器人平台。该磁性纳米机器人具有多模态成像功能，包括近红外荧光，MRI和PA，具有高移动目标效应（<5 s）。在与MET和VEGF抗体结合后，该平台可实现多分子靶向。在外加磁场作用下，双靶向（主动磁和物理磁）可以通过肿瘤成像实现体外和体内的精确肿瘤细胞靶向。此外，其近红外发光增强了实时温度检测（区域：24.6-41.5℃）。特别是，深度学习可以使NIR II成像同时增强荧光成像，用于手术边界定义和温度传感显示（R2= 0.973, RMSE = 1.50℃）。磁性/稀土混合纳米机器人具有高穿透性和高空间分辨率，在精密生物医学领域具有广阔的应用前景。

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引用次数: 0

A highly sensitive NIR-II ratiometric photoacoustic probe activated rapidly by nitric oxide for accurate in vivo imaging of liver injury 一种高灵敏度的NIR-II比率光声探针，由一氧化氮快速激活，用于准确的肝损伤体内成像

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-05-15 Epub Date: 2026-02-12 DOI: 10.1016/j.snb.2026.139654

Yidong Bin, Zongyi He, Lixian Huang, Caiying Li, Fanggui Ye, Shulin Zhao, Yong Huang, Liangliang Zhang

Nitric oxide (NO) plays key roles as a signaling molecule and an inflammatory mediator in the pathophysiology of diseases. Real-time, precise visualization of NO dynamics in deep tissues is essential for understanding disease mechanisms and evaluating therapeutic efficacy of drugs. However, existing NO probes, particularly those operating in the first near-infrared (NIR-I) window or single-wavelength “turn-on”, have limitations such as shallow penetration depth, tissue scattering, slow response and low sensitivity. To address these limitations, we used an electron cloud density enhancement strategy to develop NO-activated second near-infrared (NIR-II) window ratiometric photoacoustic (PA) probe (TCF-BA) by replacing the cationic heptamethine cyanine scaffold with an anionic counterpart. This anion-modulated strategy significantly increases electron cloud density, thereby improving the reactivity and sensitivity toward NO. The TCF-BA probe exhibits a significant ratiometric PA response upon NO activation, enabling high-contrast, deep-tissue imaging. Leveraging the high spatiotemporal resolution of PA imaging, the TCF-BA probe was used to quantitatively monitor NO fluctuations in a mouse model of liver injury. Overall, this study provides a reliable molecular imaging tool to investigate NO-related pathological processes and offers a promising strategy for the design of activatable NIR-II ratiometric probes.

一氧化氮（NO）作为一种信号分子和炎症介质在疾病的病理生理中起着关键作用。深层组织一氧化氮动力学的实时、精确可视化对于了解疾病机制和评估药物治疗效果至关重要。然而，现有的NO探针，特别是在第一个近红外（NIR-I）窗口或单波长“开启”时工作的探针，存在穿透深度浅、组织散射、响应慢和灵敏度低等局限性。为了解决这些限制，我们使用电子云密度增强策略开发了no激活的第二近红外（NIR-II）窗口比率光声（PA）探针（TCF-BA），方法是用阴离子替代阳离子七甲基菁氨酸支架。这种阴离子调制策略显著增加了电子云密度，从而提高了对NO的反应性和灵敏度。TCF-BA探针在NO激活时表现出显著的比例PA响应，从而实现高对比度的深层组织成像。利用PA成像的高时空分辨率，利用TCF-BA探针定量监测肝损伤小鼠模型中的NO波动。总之，本研究提供了一种可靠的分子成像工具来研究no相关的病理过程，并为设计可活化的NIR-II比率探针提供了一种有希望的策略。

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引用次数: 0

Lighting up POLRMT: Computational discovery of a selective and " Off–On " fluorescent probe for cancer theranostics 点亮POLRMT：用于癌症治疗的选择性和“Off-On”荧光探针的计算发现

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-05-15 Epub Date: 2026-02-12 DOI: 10.1016/j.snb.2026.139660

Weiheng Zhang , Yingli Zhu , Yanpeng Fang , Ying Yin , Qiangchao Meng , Qianhui Liu , Pian Yu , Fei Chen , Wenbin Zeng , Jie Dong

Human mitochondrial RNA polymerase (POLRMT), a critical enzyme for mitochondrial DNA transcription, is frequently overexpressed in various tumors, including colorectal cancer, where it drives cancer progression. Specific targeting of POLRMT not only holds promise for suppressing cancer cell growth but also provides a novel molecular tool for cancer diagnosis through direct visualization. However, imaging strategies for POLRMT are currently underdeveloped, which restricts its translational potential. To address this gap, we present the rational design and characterization of a novel fluorescent probe based on the Folding-Photoinduced Electron Transfer (PET) mechanism. Our approach integrated structure-driven drug design with molecular dynamics simulations. The resulting probe, BMXan–ImPy, was constructed by combining the pharmacophore recognition unit BMXan with the optical module ImPy, and its fluorescence mechanism was validated through quantum chemical calculations. In the free state, BMXan–ImPy adopts a folded conformation that facilitates PET, leading to quenched fluorescence. Upon binding to POLRMT, the interaction induces conformational unfolding, which blocks the PET process and restores fluorescence, enabling highly specific “Off–On” imaging. Experimental studies in colorectal cancer cells demonstrated that BMXan–ImPy exhibits strong biological activity and favorable optical properties. This work introduces the first POLRMT-targeted theranostic small-molecule probe, underscoring its potential as a promising strategy and technical platform for the early diagnosis and precision therapy of colorectal cancer.

人类线粒体RNA聚合酶（POLRMT）是线粒体DNA转录的关键酶，在包括结直肠癌在内的各种肿瘤中经常过度表达，在那里它驱动癌症的进展。特异性靶向POLRMT不仅有望抑制癌细胞的生长，而且为通过直接可视化诊断癌症提供了一种新的分子工具。然而，POLRMT的成像策略目前尚不发达，这限制了其转化潜力。为了解决这一空白，我们提出了一种基于折叠光致电子转移（PET）机制的新型荧光探针的合理设计和表征。我们的方法将结构驱动药物设计与分子动力学模拟相结合。将药效团识别单元BMXan与光学模块ImPy结合构建探针BMXan - ImPy，并通过量子化学计算验证其荧光机理。在自由状态下，BMXan-ImPy采用折叠构象，有利于PET，导致荧光猝灭。在与POLRMT结合后，相互作用诱导构象展开，从而阻断PET过程并恢复荧光，从而实现高度特异性的“Off-On”成像。在结直肠癌细胞中的实验研究表明，BMXan-ImPy具有很强的生物活性和良好的光学特性。本工作介绍了首个polrmt靶向治疗性小分子探针，强调了其作为结直肠癌早期诊断和精准治疗的有前途的策略和技术平台的潜力。

{"title":"Lighting up POLRMT: Computational discovery of a selective and \" Off–On \" fluorescent probe for cancer theranostics","authors":"Weiheng Zhang , Yingli Zhu , Yanpeng Fang , Ying Yin , Qiangchao Meng , Qianhui Liu , Pian Yu , Fei Chen , Wenbin Zeng , Jie Dong","doi":"10.1016/j.snb.2026.139660","DOIUrl":"10.1016/j.snb.2026.139660","url":null,"abstract":"<div><div>Human mitochondrial RNA polymerase (POLRMT), a critical enzyme for mitochondrial DNA transcription, is frequently overexpressed in various tumors, including colorectal cancer, where it drives cancer progression. Specific targeting of POLRMT not only holds promise for suppressing cancer cell growth but also provides a novel molecular tool for cancer diagnosis through direct visualization. However, imaging strategies for POLRMT are currently underdeveloped, which restricts its translational potential. To address this gap, we present the rational design and characterization of a novel fluorescent probe based on the Folding-Photoinduced Electron Transfer (PET) mechanism. Our approach integrated structure-driven drug design with molecular dynamics simulations. The resulting probe, <strong>BMXan–ImPy</strong>, was constructed by combining the pharmacophore recognition unit <strong>BMXan</strong> with the optical module <strong>ImPy</strong>, and its fluorescence mechanism was validated through quantum chemical calculations. In the free state, <strong>BMXan–ImPy</strong> adopts a folded conformation that facilitates PET, leading to quenched fluorescence. Upon binding to POLRMT, the interaction induces conformational unfolding, which blocks the PET process and restores fluorescence, enabling highly specific “Off–On” imaging. Experimental studies in colorectal cancer cells demonstrated that <strong>BMXan–ImPy</strong> exhibits strong biological activity and favorable optical properties. This work introduces the first POLRMT-targeted theranostic small-molecule probe, underscoring its potential as a promising strategy and technical platform for the early diagnosis and precision therapy of colorectal cancer.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"455 ","pages":"Article 139660"},"PeriodicalIF":3.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146169861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-mode PEF/SERS detection of Riboflavin based on TA-AuNSF/AgNS@PVA plasmonic hybrid substrate 基于TA-AuNSF/AgNS@PVA等离子体杂交底物的核黄素双模PEF/SERS检测

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-05-15 Epub Date: 2026-02-10 DOI: 10.1016/j.snb.2026.139647

Ziheng Song , Wenxuan Liu , Wenjie Hu , Yingying Deng , Meiqin Ni , Yingdong Han , Qingru Wang , Dong Zhang

Riboflavin (RF), a photosensitive and water-soluble vitamin, is critical for cellular redox metabolism and serves as an indicator for food quality monitoring. Herein, we present a thermally annealed self-assembled gold nanosphere film/silver nanosphere@polyvinyl alcohol (TA-AuNSF/AgNS@PVA) hybrid substrate that integrates plasmon-enhanced fluorescence (PEF) and surface-enhanced Raman scattering (SERS) for ultrasensitive RF detection. The substrate exploits localized surface plasmon resonance (LSPR) coupling between gold nanosphere arrays and silver nanospheres, generating high-density electromagnetic hot spots that markedly amplify local electric fields. It reveals a PEF detection limit of 7 nM with an approximately 300-fold fluorescence enhancement, as well as a SERS detection limit of 32 nM with a 0.8 × 10⁶ fold enhancement at 1344 cm⁻¹ under 633 nm excitation. In addition, the substrate demonstrates excellent uniformity, high selectivity against common interferents, and thermal stability. Its successful application to the analysis of commercial vitamin B₂ tablets further confirms the reliability and practical applicability. This dual-mode sensing platform provides a powerful and versatile tool for pharmaceutical quality control and the analysis of complex biological samples.

核黄素（Riboflavin， RF）是一种光敏水溶性维生素，对细胞氧化还原代谢至关重要，是食品质量监测的重要指标。在此，我们提出了一种热退火自组装金纳米球膜/银nanosphere@polyvinyl醇（ta - unsf /AgNS@PVA）混合衬底，该衬底集成了等离子体增强荧光（PEF）和表面增强拉曼散射（SERS），用于超灵敏射频检测。该衬底利用金纳米球阵列和银纳米球之间的局部表面等离子体共振（LSPR）耦合，产生高密度的电磁热点，显著放大局部电场。PEF检测限为7 nM，荧光增强约300倍；SERS检测限为32 nM，在633 nM激发下，1344 cm⁻¹ 增强0.8 × 10 26倍。此外，该衬底具有优异的均匀性、对常见干扰的高选择性和热稳定性。在市售维生素b2片分析中的成功应用进一步证实了该方法的可靠性和实用性。这种双模传感平台为药物质量控制和复杂生物样品的分析提供了强大而通用的工具。

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引用次数: 0