A highly sensitive ratiometric fluorescence sensing system was developed for simultaneous glucose and total cholesterol (TC) detection in whole blood using MnFe-layered double hydroxides (MnFe-LDHs) as a peroxidase mimic, combined with an o-phenylenediamine (OPD) substrate and nitrogen-doped graphene quantum dots (N-GQDs). The detection platform, an X-shaped laminated microfluidic paper-based analytical device (XL-μPAD), was fabricated via laser printing and cutting. The MnFe-LDHs' large surface area and layered structure provide a high affinity for OPD, with a Michaelis–Menten constant (KM) of 0.0127 mmol L−1. Upon placing a drop of blood on the XL-μPAD sample pad, the enzymatic reactions of glucose and TC produce H2O2, which MnFe-LDHs convert to hydroxyl radicals (•OH). These radicals oxidize OPD into fluorescent 2,3-diamino phenazine (DAP) with emission at 560 nm. Meanwhile, the N-GQDs emit fluorescence at 415 nm, which is quenched by DAP through the inner filter effect (IFE) and dynamic quenching, enabling ratiometric sensing via the intensity ratio (I560/I415). As H2O2 levels increase, a visible green emission appears, correlating with glucose and TC levels. This XL-μPAD system demonstrates promising potential as a portable device for multiplex biomarker detection and diagnostic applications.
{"title":"Ratiometric fluorometry on microfluidic paper-based analytical device for simultaneous glucose and cholesterol detection using MnFe-layered double hydroxides as peroxidase mimic","authors":"Nattasa Kitchawengkul , Akarapong Prakobkij , Rattaporn Saenmuangchin , Daniel Citterio , Duangjai Nacapricha , Purim Jarujamrus","doi":"10.1016/j.snb.2025.137671","DOIUrl":"10.1016/j.snb.2025.137671","url":null,"abstract":"<div><div>A highly sensitive ratiometric fluorescence sensing system was developed for simultaneous glucose and total cholesterol (TC) detection in whole blood using MnFe-layered double hydroxides (MnFe-LDHs) as a peroxidase mimic, combined with an <em>o</em>-phenylenediamine (OPD) substrate and nitrogen-doped graphene quantum dots (N-GQDs). The detection platform, an X-shaped laminated microfluidic paper-based analytical device (XL-μPAD), was fabricated via laser printing and cutting. The MnFe-LDHs' large surface area and layered structure provide a high affinity for OPD, with a Michaelis–Menten constant (K<sub>M</sub>) of 0.0127 mmol L<sup>−1</sup>. Upon placing a drop of blood on the XL-μPAD sample pad, the enzymatic reactions of glucose and TC produce H<sub>2</sub>O<sub>2</sub>, which MnFe-LDHs convert to hydroxyl radicals (<sup>•</sup>OH). These radicals oxidize OPD into fluorescent 2,3-diamino phenazine (DAP) with emission at 560 nm. Meanwhile, the N-GQDs emit fluorescence at 415 nm, which is quenched by DAP through the inner filter effect (IFE) and dynamic quenching, enabling ratiometric sensing via the intensity ratio (I<sub>560</sub>/I<sub>415</sub>). As H<sub>2</sub>O<sub>2</sub> levels increase, a visible green emission appears, correlating with glucose and TC levels. This XL-μPAD system demonstrates promising potential as a portable device for multiplex biomarker detection and diagnostic applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137671"},"PeriodicalIF":8.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143695768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-24DOI: 10.1016/j.snb.2025.137680
X.L. Xu, M.Y. Wang, H.T. Jiang, W.W. Liu, G.R. Sun, W. Ma
The high working temperature of metal oxide semiconductors hinders its application in flexible sensing devices. In this paper, a UV-activated flexible sensor operating at room temperature (RT, 25℃) is first reported based on SnO2 nanoparticles embedded in photo-catalytic TiO2 nanorods matrix. The UV-irradiated SnO2/TiO2 sensor exhibits excellent selectivity (109 ppm, 37%) to CH3COOH and reduced response/recovery time (12 s/41 s). The excellent RT flexible sensing properties are attributed to the synergistic action of UV-365 nm irradiation, the hetero-embedded microstructure and innovative terpineol binder. UV irradiation generates substantial - pairs, which serve as active sites to promote the chemisorption and redox reactions. Additional activation energy helps to improve adsorption/desorption kinetics. The heterojunction provides abundant channels, facilitating the separation and transfer of photogenerated carriers. The effect of relative humidity (RH) and temperature variations around RT on the sensor response towards CH3COOH are investigated. Under low-RH (35%-45%) and high-RH conditions (above 45%), 1% RH change has the same effect on the sensor response than 7.2 ppm and 59.4 ppm CH3COOH, respectively. 1°C temperature change exhibits the same effect than 17.9 ppm CH3COOH. Such a noise effect is by far not suitable for real-world applications. In addition, the response to 109 ppm CO reaches up to 89.5. Therefore, the sensor is seriously disturbed by CO and further research is needed. Above all, UV assistants, hetero-composites combining with innovative microstructure design are expected to be a collaborative enhancement strategy for MOSs-based RT flexible sensors.
{"title":"UV Enhanced SnO2/TiO2 Nanorods-based Flexible Room Temperature Sensor by Tuning Interfacial Chemistry and Microstructure","authors":"X.L. Xu, M.Y. Wang, H.T. Jiang, W.W. Liu, G.R. Sun, W. Ma","doi":"10.1016/j.snb.2025.137680","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137680","url":null,"abstract":"The high working temperature of metal oxide semiconductors hinders its application in flexible sensing devices. In this paper, a UV-activated flexible sensor operating at room temperature (RT, 25℃) is first reported based on SnO<sub>2</sub> nanoparticles embedded in photo-catalytic TiO<sub>2</sub> nanorods matrix. The UV-irradiated SnO<sub>2</sub>/TiO<sub>2</sub> sensor exhibits excellent selectivity (109 ppm, 37%) to CH<sub>3</sub>COOH and reduced response/recovery time (12<!-- --> <!-- -->s/41<!-- --> <!-- -->s). The excellent RT flexible sensing properties are attributed to the synergistic action of UV-365 nm irradiation, the hetero-embedded microstructure and innovative terpineol binder. UV irradiation generates substantial <span><math><msubsup is=\"true\"><mrow is=\"true\"><mi is=\"true\" mathvariant=\"normal\">e</mi></mrow><mrow is=\"true\"><mi is=\"true\" mathvariant=\"normal\">h</mi><mi is=\"true\" mathvariant=\"normal\">υ</mi></mrow><mrow is=\"true\"><mo is=\"true\">−</mo></mrow></msubsup></math></span>-<span><math><msubsup is=\"true\"><mrow is=\"true\"><mi is=\"true\" mathvariant=\"normal\">h</mi></mrow><mrow is=\"true\"><mi is=\"true\" mathvariant=\"normal\">h</mi><mi is=\"true\" mathvariant=\"normal\">υ</mi></mrow><mrow is=\"true\"><mo is=\"true\">+</mo></mrow></msubsup></math></span> pairs, which serve as active sites to promote the chemisorption and redox reactions. Additional activation energy helps to improve adsorption/desorption kinetics. The heterojunction provides abundant channels, facilitating the separation and transfer of photogenerated carriers. The effect of relative humidity (RH) and temperature variations around RT on the sensor response towards CH<sub>3</sub>COOH are investigated. Under low-RH (35%-45%) and high-RH conditions (above 45%), 1% RH change has the same effect on the sensor response than 7.2 ppm and 59.4 ppm CH<sub>3</sub>COOH, respectively. 1°C temperature change exhibits the same effect than 17.9 ppm CH<sub>3</sub>COOH. Such a noise effect is by far not suitable for real-world applications. In addition, the response to 109 ppm CO reaches up to 89.5. Therefore, the sensor is seriously disturbed by CO and further research is needed. Above all, UV assistants, hetero-composites combining with innovative microstructure design are expected to be a collaborative enhancement strategy for MOSs-based RT flexible sensors.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"35 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143695766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-24DOI: 10.1016/j.snb.2025.137646
Haiyan Ma, Guojie Li, Huihui Zhang, Xinyu Wang, Fengyun Li, Jing Yan, Liu Hong, Yuewen Zhang, Qiaosheng Pu
Rapid and sensitive detection of foodborne pathogens is essential for ensuring food safety and protecting public health. In this study, we developed an innovative microfluidic fluorescence digital analysis platform enhanced by deep learning to detect pathogens at ultra-low concentrations. The biosensor features a staggered herringbone double-spiral (SHDS) microfluidic design, seamlessly integrating bacteria capture, detection, and release processes using Quantum dot (QD)-Aptamer conjugates for precise identification. Fluorescence image analysis, powered by a Resnet-18-based convolutional neural networks (CNN), directly quantifies Escherichia coli (E. coli) concentrations from fluorescence images, streamlining data processing and increasing sensitivity. The platform offers a linear detection range from 10 to 3 × 10⁶ CFU/mL (R² = 0.990), achieves capture efficiencies of up to 100% at low bacterial concentrations (4 × 10² CFU/mL), and offers an ultra-low detection limit of 2 CFU/mL within just 1.5 hours. The CNN model effectively filters out background noise and interferences, achieving over 99% predictive accuracy. Validation using milk and chicken samples resulted in high recovery rates (96.7% to 104.0%). This biosensor presents a rapid, reliable, and practical solution for pathogen detection in complex food matrices, significantly improving food safety and security.
{"title":"Rapid and Ultra-Sensitive Detection of Foodborne Pathogens by Deep Learning-Enhanced Microfluidic Biosensing","authors":"Haiyan Ma, Guojie Li, Huihui Zhang, Xinyu Wang, Fengyun Li, Jing Yan, Liu Hong, Yuewen Zhang, Qiaosheng Pu","doi":"10.1016/j.snb.2025.137646","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137646","url":null,"abstract":"Rapid and sensitive detection of foodborne pathogens is essential for ensuring food safety and protecting public health. In this study, we developed an innovative microfluidic fluorescence digital analysis platform enhanced by deep learning to detect pathogens at ultra-low concentrations. The biosensor features a staggered herringbone double-spiral (SHDS) microfluidic design, seamlessly integrating bacteria capture, detection, and release processes using Quantum dot (QD)-Aptamer conjugates for precise identification. Fluorescence image analysis, powered by a Resnet-18-based convolutional neural networks (CNN), directly quantifies <em>Escherichia coli (E. coli)</em> concentrations from fluorescence images, streamlining data processing and increasing sensitivity. The platform offers a linear detection range from 10 to 3 × 10⁶ CFU/mL (R² = 0.990), achieves capture efficiencies of up to 100% at low bacterial concentrations (4 × 10² CFU/mL), and offers an ultra-low detection limit of 2 CFU/mL within just 1.5<!-- --> <!-- -->hours. The CNN model effectively filters out background noise and interferences, achieving over 99% predictive accuracy. Validation using milk and chicken samples resulted in high recovery rates (96.7% to 104.0%). This biosensor presents a rapid, reliable, and practical solution for pathogen detection in complex food matrices, significantly improving food safety and security.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"57 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Human chorionic gonadotropin (hCG) is an essential biomarker for pregnancy and seminal system disorders, with elevated levels linked to tumor progression. Early and accurate detection of hCG is critical for disease diagnosis, necessitating the development of highly sensitive and selective assays. Self-powered photoelectrochemical (PEC) biosensors, which function without an external power source at zero bias voltage, offer superior anti-interference properties and have emerged as a promising approach for biomarker detection. This study presents the synthesis of MIL-88A-derived NiFe LDH double-shell nanocages integrated with titanium dioxide (TiO2) and phosphorus-doped graphitic carbon nitride (PCN). The resulting Ni-Fe LDH DSNC/TiO2/PCN heterostructure was immobilized on a fluorine-doped tin oxide (FTO) substrate for efficient hCG detection under zero-bias conditions (0 V vs. Ag/AgCl). Ascorbic acid (AA) acts as an electron donor to scavenge photogenerated holes, thereby improving the sensitivity of the PEC immunosensor. Compared to bare FTO, the Ni-Fe LDH DSNC/TiO2/PCN heterostructure exhibited a significantly higher PEC signal, attributed to its enhanced photoelectric conversion efficiency, lower charge recombination, and effective charge separation. The PEC immunosensor shows a linear response to hCG (0.0001–100 ng/mL) with a detection limit of 0.087 pg/mL. The superior performance of the Ni-Fe LDH DSNC/TiO2/PCN-based PEC immunosensor can be attributed to its optimized bandgap, efficient charge transport, broad visible-light absorption, and large surface area. Furthermore, the PEC sensor exhibited excellent stability, selectivity, and reproducibility, with high recovery rates in real urine sample analysis, demonstrating its potential for reliable hCG detection.
{"title":"Self-powered photoelectrochemical immunosensor using MIL-88A-derived NiFe LDH double-shell nanocages/TiO2/P-doped graphitic carbon nitride heterostructure for hCG detection in urine samples of pregnant and non-pregnant women","authors":"Rajalakshmi Sakthivel, Yu-Ting Liao, Subbiramaniyan Kubendhiran, Yu-Chien Lin, Lu-Yin Lin, Yeh-Fang Duann, Da-Hua Wei, Ren-Jei Chung","doi":"10.1016/j.snb.2025.137670","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137670","url":null,"abstract":"Human chorionic gonadotropin (hCG) is an essential biomarker for pregnancy and seminal system disorders, with elevated levels linked to tumor progression. Early and accurate detection of hCG is critical for disease diagnosis, necessitating the development of highly sensitive and selective assays. Self-powered photoelectrochemical (PEC) biosensors, which function without an external power source at zero bias voltage, offer superior anti-interference properties and have emerged as a promising approach for biomarker detection. This study presents the synthesis of MIL-88A-derived NiFe LDH double-shell nanocages integrated with titanium dioxide (TiO<sub>2</sub>) and phosphorus-doped graphitic carbon nitride (PCN). The resulting Ni-Fe LDH DSNC/TiO<sub>2</sub>/PCN heterostructure was immobilized on a fluorine-doped tin oxide (FTO) substrate for efficient hCG detection under <em>zero</em>-bias conditions (0<!-- --> <!-- -->V <em>vs. Ag/AgCl</em>). Ascorbic acid (AA) acts as an electron donor to scavenge photogenerated holes, thereby improving the sensitivity of the PEC immunosensor. Compared to bare FTO, the Ni-Fe LDH DSNC/TiO<sub>2</sub>/PCN heterostructure exhibited a significantly higher PEC signal, attributed to its enhanced photoelectric conversion efficiency, lower charge recombination, and effective charge separation. The PEC immunosensor shows a linear response to hCG (0.0001–100<!-- --> <!-- -->ng/mL) with a detection limit of 0.087<!-- --> <!-- -->pg/mL. The superior performance of the Ni-Fe LDH DSNC/TiO<sub>2</sub>/PCN-based PEC immunosensor can be attributed to its optimized bandgap, efficient charge transport, broad visible-light absorption, and large surface area. Furthermore, the PEC sensor exhibited excellent stability, selectivity, and reproducibility, with high recovery rates in real urine sample analysis, demonstrating its potential for reliable hCG detection.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"88 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-23DOI: 10.1016/j.snb.2025.137629
Mhamad Chrayteh, Fabien Simon, Francis Hindle, Gaël Mouret, Anthony Roucou, Manuel Goubet, Julien Mory, Christelle Nicollet, Arnaud Cuisset
Cavity-Enhanced Absorption Spectroscopy (CEAS) and Cavity Ring-Down Spectroscopy (CRDS) are well established for sensitive infrared measurements of gas-phase compounds at trace levels using their rovibrational signatures. The recent successful development of a THz Fabry–Perot spectrometer by Hindle et al. (2019) shows that the adaptation of such techniques to submillimeter wavelengths allows to probe rotational transitions of light polar compounds. Here we report on the development of a new millimeter-wave resonator, covering the 150-215 GHz frequency range, and based on a low-loss corrugated waveguide with homemade highly reflective photonic mirrors obtaining a finesse above 3000 at around 164 GHz. With an effective path length of two kilometers, a significant sensitivity has been evaluated, and the detection of semi-volatile organic vapors at a trace level may be now envisaged at room temperature. We applied this technology to detect gas-phase explosive taggants and precursors, confirming a detection limit of 2 ppmv for nitromethane (NM). Leveraging the unique characteristics of the millimeter wave frequency band, we showcase highly selective detection in quasi-realistic environments of complex chemical mixtures involving explosive taggants with close chemical structures such as nitrotoluene isomers. Furthermore, we successfully address the challenge of detecting these nitro-derivative compounds vaporized from model matrices: KCl matrices, granular and plastic (NP91) explosives respectively in conventional and pyrotechnic laboratories. Our findings underscore this approach as a potent tool for practical explosive detection applications.
{"title":"Millimeter-wave measurements in high finesse cavity of nitro-derivatives traces: A new insight in the explosive vapor sensing","authors":"Mhamad Chrayteh, Fabien Simon, Francis Hindle, Gaël Mouret, Anthony Roucou, Manuel Goubet, Julien Mory, Christelle Nicollet, Arnaud Cuisset","doi":"10.1016/j.snb.2025.137629","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137629","url":null,"abstract":"Cavity-Enhanced Absorption Spectroscopy (CEAS) and Cavity Ring-Down Spectroscopy (CRDS) are well established for sensitive infrared measurements of gas-phase compounds at trace levels using their rovibrational signatures. The recent successful development of a THz Fabry–Perot spectrometer by Hindle et al. (2019) shows that the adaptation of such techniques to submillimeter wavelengths allows to probe rotational transitions of light polar compounds. Here we report on the development of a new millimeter-wave resonator, covering the 150-215 GHz frequency range, and based on a low-loss corrugated waveguide with homemade highly reflective photonic mirrors obtaining a finesse above 3000 at around 164 GHz. With an effective path length of two kilometers, a significant sensitivity has been evaluated, and the detection of semi-volatile organic vapors at a trace level may be now envisaged at room temperature. We applied this technology to detect gas-phase explosive taggants and precursors, confirming a detection limit of 2 ppmv for nitromethane (NM). Leveraging the unique characteristics of the millimeter wave frequency band, we showcase highly selective detection in quasi-realistic environments of complex chemical mixtures involving explosive taggants with close chemical structures such as nitrotoluene isomers. Furthermore, we successfully address the challenge of detecting these nitro-derivative compounds vaporized from model matrices: KCl matrices, granular and plastic (NP91) explosives respectively in conventional and pyrotechnic laboratories. Our findings underscore this approach as a potent tool for practical explosive detection applications.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"90 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143677927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rapid and accurate identification of infectious disease pathogens plays a key role in emergency preparedness and respond to a wide range of biosecurity threats. However, current strategies require dedicated laboratories, expensive instruments and skilled operators, which are not suitable for point-of-care testing. Here, we developed an integrated CRISPR-based microfluidic platform that consisted of 3D printed bioreactors and a homemade miniaturized heater for rapid and sensitive nucleic acids detection. The all-in-one, containment-free bioreactors combined recombinase polymerase amplification, CRISPR and lateral flow dipsticks (RPA-CRISPR-LFD), allowing user-friendly operation and eliminating the need for complex laboratory equipment. The RPA-CRISPR-LFD assay was able to efficiently detect plasmids of monkeypox virus (MPXV) and lumpy skin disease virus (LSDV) down to 1 copy <span><span style=""></span><span data-mathml='<math xmlns="http://www.w3.org/1998/Math/MathML"><mrow is="true"><mi is="true">μ</mi><msup is="true"><mrow is="true"><mi is="true">L</mi></mrow><mrow is="true"><mo is="true">−</mo><mn is="true">1</mn></mrow></msup></mrow></math>' role="presentation" style="font-size: 90%; display: inline-block; position: relative;" tabindex="0"><svg aria-hidden="true" focusable="false" height="2.894ex" role="img" style="vertical-align: -0.697ex;" viewbox="0 -945.9 2289.4 1246" width="5.317ex" xmlns:xlink="http://www.w3.org/1999/xlink"><g fill="currentColor" stroke="currentColor" stroke-width="0" transform="matrix(1 0 0 -1 0 0)"><g is="true"><g is="true"><use xlink:href="#MJMATHI-3BC"></use></g><g is="true" transform="translate(603,0)"><g is="true"><g is="true"><use xlink:href="#MJMATHI-4C"></use></g></g><g is="true" transform="translate(681,410)"><g is="true"><use transform="scale(0.707)" xlink:href="#MJMAIN-2212"></use></g><g is="true" transform="translate(550,0)"><use transform="scale(0.707)" xlink:href="#MJMAIN-31"></use></g></g></g></g></g></svg><span role="presentation"><math xmlns="http://www.w3.org/1998/Math/MathML"><mrow is="true"><mi is="true">μ</mi><msup is="true"><mrow is="true"><mi is="true">L</mi></mrow><mrow is="true"><mo is="true">−</mo><mn is="true">1</mn></mrow></msup></mrow></math></span></span><script type="math/mml"><math><mrow is="true"><mi is="true">μ</mi><msup is="true"><mrow is="true"><mi is="true">L</mi></mrow><mrow is="true"><mo is="true">−</mo><mn is="true">1</mn></mrow></msup></mrow></math></script></span>. The platform can identify 5 extraction-free samples in parallel within 30 min, significantly reducing time consumption. In addition, we validated its clinical utility and versatility by testing 67 simulated blood or swab samples of MPXV and LSDV, yielding results consistent with real-time PCR. Thus, the platform enables rapid and convenient detection of diverse pathogens, and with the futur
{"title":"A portable all-in-one microfluidic platform integrated with CRISPR-based extraction-free assay for rapid and on-site detection of monkeypox and lumpy skin disease","authors":"Yizheng Huang, Yuhan Lu, Xiaofei Liu, Menghao Chai, Ling Yang, Kun Yin, Jiayao He, Zhijie Wang, Yajun Zhang, Yude Yu, Songyin Qiu, Yiqiang Fan, Zhao Li","doi":"10.1016/j.snb.2025.137612","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137612","url":null,"abstract":"Rapid and accurate identification of infectious disease pathogens plays a key role in emergency preparedness and respond to a wide range of biosecurity threats. However, current strategies require dedicated laboratories, expensive instruments and skilled operators, which are not suitable for point-of-care testing. Here, we developed an integrated CRISPR-based microfluidic platform that consisted of 3D printed bioreactors and a homemade miniaturized heater for rapid and sensitive nucleic acids detection. The all-in-one, containment-free bioreactors combined recombinase polymerase amplification, CRISPR and lateral flow dipsticks (RPA-CRISPR-LFD), allowing user-friendly operation and eliminating the need for complex laboratory equipment. The RPA-CRISPR-LFD assay was able to efficiently detect plasmids of monkeypox virus (MPXV) and lumpy skin disease virus (LSDV) down to 1 copy <span><span style=\"\"></span><span data-mathml='<math xmlns=\"http://www.w3.org/1998/Math/MathML\"><mrow is=\"true\"><mi is=\"true\">&#x3BC;</mi><msup is=\"true\"><mrow is=\"true\"><mi is=\"true\">L</mi></mrow><mrow is=\"true\"><mo is=\"true\">&#x2212;</mo><mn is=\"true\">1</mn></mrow></msup></mrow></math>' role=\"presentation\" style=\"font-size: 90%; display: inline-block; position: relative;\" tabindex=\"0\"><svg aria-hidden=\"true\" focusable=\"false\" height=\"2.894ex\" role=\"img\" style=\"vertical-align: -0.697ex;\" viewbox=\"0 -945.9 2289.4 1246\" width=\"5.317ex\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g fill=\"currentColor\" stroke=\"currentColor\" stroke-width=\"0\" transform=\"matrix(1 0 0 -1 0 0)\"><g is=\"true\"><g is=\"true\"><use xlink:href=\"#MJMATHI-3BC\"></use></g><g is=\"true\" transform=\"translate(603,0)\"><g is=\"true\"><g is=\"true\"><use xlink:href=\"#MJMATHI-4C\"></use></g></g><g is=\"true\" transform=\"translate(681,410)\"><g is=\"true\"><use transform=\"scale(0.707)\" xlink:href=\"#MJMAIN-2212\"></use></g><g is=\"true\" transform=\"translate(550,0)\"><use transform=\"scale(0.707)\" xlink:href=\"#MJMAIN-31\"></use></g></g></g></g></g></svg><span role=\"presentation\"><math xmlns=\"http://www.w3.org/1998/Math/MathML\"><mrow is=\"true\"><mi is=\"true\">μ</mi><msup is=\"true\"><mrow is=\"true\"><mi is=\"true\">L</mi></mrow><mrow is=\"true\"><mo is=\"true\">−</mo><mn is=\"true\">1</mn></mrow></msup></mrow></math></span></span><script type=\"math/mml\"><math><mrow is=\"true\"><mi is=\"true\">μ</mi><msup is=\"true\"><mrow is=\"true\"><mi is=\"true\">L</mi></mrow><mrow is=\"true\"><mo is=\"true\">−</mo><mn is=\"true\">1</mn></mrow></msup></mrow></math></script></span>. The platform can identify 5 extraction-free samples in parallel within 30 min, significantly reducing time consumption. In addition, we validated its clinical utility and versatility by testing 67 simulated blood or swab samples of MPXV and LSDV, yielding results consistent with real-time PCR. Thus, the platform enables rapid and convenient detection of diverse pathogens, and with the futur","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"12 5 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-22DOI: 10.1016/j.snb.2025.137666
Yunshan Zhang, Sisi Bu, Fang Yang, Tuo Huang, Hao Dong, Jing Ye, Wenlin Xie, Xianzhong Feng, Diming Zhang
The subtle free energy difference introduced by a single nucleotide mutation results in poor specificity of almost all DNA hybridization probe-based single nucleotide polymorphism (SNP) detection techniques. The development of SNP biosensing strategies with both specificity and sensitivity is a hot and difficult issue in the current field. In this study, we creatively constructed a competitive toehold-mediated strand displacement sensing platform (CTMSD) based on the traditional TMSD reaction, which increased the energy barrier through the intrinsic competition mechanism and expanded the detection window of SNPs. Furthermore, based on the characteristics of the CTMSD platform, the dual-signal detection mode was introduced to change the function model of the detection curve through reporting internal reference ratio signal. The new detection curve model not only compensated for sensitivity, significantly enhanced the discrimination factor, but also greatly expanded the detection window with infinite robustness factor over the detection range. The expansion of the detection window and the improvement of specificity of CTMSD for SNP recognition based on the ratiometric signal output model were verified by computer simulations and experiments. In addition, as a deformation of the strand displacement reaction, the CTMSD was readily adaptable to commonly used signal amplification techniques, such as catalytic hairpin assembly (CHA). Through the CTMSD-CHA performance analysis and real testing of cell genomic samples, the practical application value of CTMSD with the ratiometric signal output model was confirmed. This study provides an important reference for the design and improvement of SNP biosensors and even for all nucleic acid biosensors.
{"title":"Expand detection windows for identifying single nucleotide polymorphisms using a competitive toehold-mediated strand displacement ratiometric sensing platform","authors":"Yunshan Zhang, Sisi Bu, Fang Yang, Tuo Huang, Hao Dong, Jing Ye, Wenlin Xie, Xianzhong Feng, Diming Zhang","doi":"10.1016/j.snb.2025.137666","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137666","url":null,"abstract":"The subtle free energy difference introduced by a single nucleotide mutation results in poor specificity of almost all DNA hybridization probe-based single nucleotide polymorphism (SNP) detection techniques. The development of SNP biosensing strategies with both specificity and sensitivity is a hot and difficult issue in the current field. In this study, we creatively constructed a competitive toehold-mediated strand displacement sensing platform (CTMSD) based on the traditional TMSD reaction, which increased the energy barrier through the intrinsic competition mechanism and expanded the detection window of SNPs. Furthermore, based on the characteristics of the CTMSD platform, the dual-signal detection mode was introduced to change the function model of the detection curve through reporting internal reference ratio signal. The new detection curve model not only compensated for sensitivity, significantly enhanced the discrimination factor, but also greatly expanded the detection window with infinite robustness factor over the detection range. The expansion of the detection window and the improvement of specificity of CTMSD for SNP recognition based on the ratiometric signal output model were verified by computer simulations and experiments. In addition, as a deformation of the strand displacement reaction, the CTMSD was readily adaptable to commonly used signal amplification techniques, such as catalytic hairpin assembly (CHA). Through the CTMSD-CHA performance analysis and real testing of cell genomic samples, the practical application value of CTMSD with the ratiometric signal output model was confirmed. This study provides an important reference for the design and improvement of SNP biosensors and even for all nucleic acid biosensors.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"27 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Colorectal cancer (CRC) represents a significant global health issue, necessitating innovative approaches for early screening and diagnosis. Recent advances in molecular diagnostics have highlighted the potential of aptamers for use as highly specific and sensitive probes for detecting cancer biomarkers. In this study, we focus on the identification of aptamers that selectively bind to Solobacterium moorei (S. moorei), a bacterium associated with CRC. By integrating these aptamers into electrochemical sensor platforms, a reliable diagnostic tool is created for facile implementation in the clinical setting. More specifically, nucleic acid aptamers for S. moorei were obtained through whole-cell SELEX, and the affinity and specificity of these aptamers were validated. Subsequently, two types of electrochemical sensors were developed. Firstly, an electrochemical impedance spectroscopy sensor was developed to detect impedance changes on the electrode surface, which were caused by the binding of S. moorei to the aptamer. Secondly, a CRISPR/Cas12a-based electrochemical aptasensor was developed based on the ability of the Cas12a enzyme to be activated by specific single-stranded DNA, triggering its trans-cleavage activity. The limits of detection of these sensors for S. moorei were 30 and 6 CFU/mL, respectively. Clinical validation was performed using patient samples to assess the sensor efficacy in a real-world setting. The obtained results suggested that the abundance of S. moorei in the feces of CRC patients was significantly greater compared to that of healthy individuals. This integration of S. moorei aptamers into electrochemical sensors offers a non-invasive and cost-effective alternative to current screening methods available for CRC.
{"title":"Screening and Diagnosis of Colorectal Cancer Using Nucleic Acid Aptamers Targeting Solobacterium moorei: Development of Electrochemical Sensors for Clinical Application","authors":"Decai Yuan, Cheng Qiu, Hui Chen, Feng Liu, Shanshan Feng, Peiyi Zhang, Ying Qin, Tingting Fan, Yan Chen, Yuyang Jiang","doi":"10.1016/j.snb.2025.137669","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137669","url":null,"abstract":"Colorectal cancer (CRC) represents a significant global health issue, necessitating innovative approaches for early screening and diagnosis. Recent advances in molecular diagnostics have highlighted the potential of aptamers for use as highly specific and sensitive probes for detecting cancer biomarkers. In this study, we focus on the identification of aptamers that selectively bind to <em>Solobacterium moorei (S. moorei)</em>, a bacterium associated with CRC. By integrating these aptamers into electrochemical sensor platforms, a reliable diagnostic tool is created for facile implementation in the clinical setting. More specifically, nucleic acid aptamers for <em>S. moorei</em> were obtained through whole-cell SELEX, and the affinity and specificity of these aptamers were validated. Subsequently, two types of electrochemical sensors were developed. Firstly, an electrochemical impedance spectroscopy sensor was developed to detect impedance changes on the electrode surface, which were caused by the binding of <em>S. moorei</em> to the aptamer. Secondly, a CRISPR/Cas12a-based electrochemical aptasensor was developed based on the ability of the Cas12a enzyme to be activated by specific single-stranded DNA, triggering its trans-cleavage activity. The limits of detection of these sensors for <em>S. moorei</em> were 30 and 6 CFU/mL, respectively. Clinical validation was performed using patient samples to assess the sensor efficacy in a real-world setting. The obtained results suggested that the abundance of <em>S. moorei</em> in the feces of CRC patients was significantly greater compared to that of healthy individuals. This integration of <em>S. moorei</em> aptamers into electrochemical sensors offers a non-invasive and cost-effective alternative to current screening methods available for CRC.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"46 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-22DOI: 10.1016/j.snb.2025.137667
Zhihao Li, Xue Wang, Xiaojuan Wang, Yang Shu
DNA nanodevices based on nucleic acid aptamers have become powerful tools for tumor imaging. However, the problem of “on-target, off-tumor” has always been a challenge for intelligent molecular probes to identify tumors. In order to achieve precise identification and tumor microenvironment (TEM) localization of cancer cells, we integrated membrane protein aptamers with two reporter molecules, imotif (pH reporter) and ATP aptamer (ATP reporter), which can respond to specific biomarkers in TME, and developed a Y-shaped multitasking processor (Y-smp). This Y-shaped multitasking processor can target membrane protein overexpressed on the surface of cancer cells, allowing them to remain on the cell surface. At the same time, the two reporter probes work independently in response to acidity and ATP, undergo conformational changes and detach from Y-smp, carrying away quenching groups to produce green fluorescence signals corresponding to ATP molecules and red fluorescence signals corresponding to acidity. The Y-shaped multitasking processor can generate different fluorescent signals corresponding to two microenvironment markers, and does not interfere with each other in a simulated tumor microenvironment characterized by weak acidity and abundant ATP. The simple and intuitive molecular probe recognition strategy provides the possibility for precise localization and recognition of cancer cells.
{"title":"A membrane-protein targeted DNA multitasking processor for precise tumor cell imaging","authors":"Zhihao Li, Xue Wang, Xiaojuan Wang, Yang Shu","doi":"10.1016/j.snb.2025.137667","DOIUrl":"https://doi.org/10.1016/j.snb.2025.137667","url":null,"abstract":"DNA nanodevices based on nucleic acid aptamers have become powerful tools for tumor imaging. However, the problem of “on-target, off-tumor” has always been a challenge for intelligent molecular probes to identify tumors. In order to achieve precise identification and tumor microenvironment (TEM) localization of cancer cells, we integrated membrane protein aptamers with two reporter molecules, imotif (pH reporter) and ATP aptamer (ATP reporter), which can respond to specific biomarkers in TME, and developed a Y-shaped multitasking processor (Y-smp). This Y-shaped multitasking processor can target membrane protein overexpressed on the surface of cancer cells, allowing them to remain on the cell surface. At the same time, the two reporter probes work independently in response to acidity and ATP, undergo conformational changes and detach from Y-smp, carrying away quenching groups to produce green fluorescence signals corresponding to ATP molecules and red fluorescence signals corresponding to acidity. The Y-shaped multitasking processor can generate different fluorescent signals corresponding to two microenvironment markers, and does not interfere with each other in a simulated tumor microenvironment characterized by weak acidity and abundant ATP. The simple and intuitive molecular probe recognition strategy provides the possibility for precise localization and recognition of cancer cells.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"70 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-22DOI: 10.1016/j.snb.2025.137668
Xiaoqi Hu , Kiseok Han , Gyuho Choi , Myungeun Suk , Tae-Jin Kim
Fluorescence resonance energy transfer (FRET)-based live-cell imaging and genetically encoded biosensors are widely used to study biochemical processes in living cells. However, there remains a gap in robust analytical methods for examining complex processes like endosomal trafficking. In this study, we introduce two advanced tools—EasyFRET and EndoAna—specifically designed for FRET image analysis, along with two novel potassium biosensors, GalT-KIRIN and KIRIN-Lamp1, targeted to endosomes and lysosomes, respectively. Together, these tools establish a comprehensive workflow for investigating potassium signaling, which plays a critical role in cellular homeostasis and various biological functions. Our live-cell imaging results demonstrate that EasyFRET and EndoAna provide efficient and reliable FRET image analysis, while the potassium indicators offer valuable insights into potassium dynamics within the endosomal trafficking system.
{"title":"Illuminating potassium dynamics in endocytic pathways using FRET biosensors and computational tools","authors":"Xiaoqi Hu , Kiseok Han , Gyuho Choi , Myungeun Suk , Tae-Jin Kim","doi":"10.1016/j.snb.2025.137668","DOIUrl":"10.1016/j.snb.2025.137668","url":null,"abstract":"<div><div>Fluorescence resonance energy transfer (FRET)-based live-cell imaging and genetically encoded biosensors are widely used to study biochemical processes in living cells. However, there remains a gap in robust analytical methods for examining complex processes like endosomal trafficking. In this study, we introduce two advanced tools—EasyFRET and EndoAna—specifically designed for FRET image analysis, along with two novel potassium biosensors, GalT-KIRIN and KIRIN-Lamp1, targeted to endosomes and lysosomes, respectively. Together, these tools establish a comprehensive workflow for investigating potassium signaling, which plays a critical role in cellular homeostasis and various biological functions. Our live-cell imaging results demonstrate that EasyFRET and EndoAna provide efficient and reliable FRET image analysis, while the potassium indicators offer valuable insights into potassium dynamics within the endosomal trafficking system.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137668"},"PeriodicalIF":8.0,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143675279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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