Sensors and Actuators B: Chemical最新文献_第5页

Ultrasensitive dual-signal electrochemical aptasensor for multiplexed detection of HER2 and EGFR toward breast cancer subtyping 超灵敏双信号电化学传感器多重检测HER2和EGFR对乳腺癌亚型的影响

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-07 DOI: 10.1016/j.snb.2026.139445

Mingang Liao , Yulu Zhang , Ying Ma , Kaibin Xie , Yudan Hao , Zhaocheng Wen , Jing Lu , Peng Duan , Duanping Sun , Shengen Wu

Breast cancer is a leading cause of cancer-related mortality in women, and the expression profiles of specific tumor biomarkers are strongly associated with unfavorable prognosis. Herein, we engineered a dual-signal electrochemical aptasensor for the simultaneous quantification of clinically critical cancer biomarkers EGFR and HER2, enabling precise tumor phenotype characterization. The platform integrates two innovative probes: Fe₃O₄@UiO-66/Au functionalized with methylene blue/ferrocene-labeled aptamers (Probe1) for magnetic capture and signal amplification; aptamer-functionalized DNA nanotetrahedron (Probe2)-modified screen-printed carbon electrode for enhanced target recognition. The “Probe1-target-Probe2” sandwich architecture generates distinct electrochemical signals, achieving ultrahigh sensitivity (limit of detection: EGFR: 0.035 pg/mL; HER2: 0.043 pg/mL) with robust serum recovery. The electrochemical biosensor directly quantified EGFR/HER2 expression on breast cancer cells with results perfectly correlated with the highly expensive and experience-dependent flow cytometry for subtype stratification (MCF-7 cell: low EGFR/high HER2; MDA-MB-231 cell: high EGFR/low HER2; SK-BR-3 cell: moderate EGFR/HER2). Remarkably, the aptasensor achieved an ultra-low detection limit of 3 cells/mL for MCF-7, MDA-MB-231, and SK-BR-3 cells. This platform offers transformative advantages over conventional methods, including cost-effectiveness, rapid analysis, eliminating fluorescent tags, and a wide linear dynamic range. This work establishes a highly practical and sensitive platform for tumor marker profiling and electrochemical characterization of breast cancer subtypes.

乳腺癌是女性癌症相关死亡的主要原因，特定肿瘤生物标志物的表达谱与不良预后密切相关。在此，我们设计了一种双信号电化学配体传感器，用于同时定量临床关键癌症生物标志物EGFR和HER2，从而实现精确的肿瘤表型表征。该平台集成了两种创新探针：Fe3O4@UiO-66/Au功能化亚甲基蓝/二茂铁标记适配体（Probe1），用于磁捕获和信号放大；适配体功能化DNA纳米四面体（Probe2）修饰的丝网印刷碳电极用于增强目标识别。“Probe1-target-Probe2”夹层结构产生不同的电化学信号，实现超高灵敏度（检测限：EGFR: 0.035 pg/mL; HER2: 0.043 pg/mL），血清回收率高。电化学生物传感器直接定量乳腺癌细胞的EGFR/HER2表达，结果与昂贵且依赖经验的流式细胞术进行亚型分层（MCF-7细胞：低EGFR/高HER2； MDA-MB-231细胞：高EGFR/低HER2； SK-BR-3细胞：中等EGFR/HER2）完全相关。值得注意的是，该适配体传感器对MCF-7、MDA-MB-231和SK-BR-3细胞的检测限达到了3个细胞/mL的超低检测限。与传统方法相比，该平台具有变革性优势，包括成本效益、快速分析、消除荧光标记和宽线性动态范围。这项工作为乳腺癌亚型的肿瘤标志物分析和电化学表征建立了一个高度实用和敏感的平台。

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引用次数: 0

Defective porphyrin Zr metal–organic framework cube decorated with platinum: Synthesization and application for colorimetry/smartphone sensing of tetracycline 铂修饰的缺陷卟啉Zr金属-有机框架立方体：合成及其在四环素比色/智能手机传感中的应用

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-07 DOI: 10.1016/j.snb.2026.139449

Yinhui Yi , Jing Li , Chenxu Wang , Guanghua Mao , Gangbing Zhu

Noble metal-based oxidase-like (MOD) nanozymes show great applications referring to the common peroxidase-like (POD) nanozymes, while their insufficient activity and stability need to be addressed urgently. Herein, by introducing defective porphyrin Zr metal–organic framework (PCN-224) cube as metal carrier and in-situ growth method, a novel MOD nanozyme of defective PCN-224 decorated with platinum nanoparticles (PCN-224@Pt NPs) was prepared and characterized firstly, which possesses extremely high loading amount of Pt (72.82 wt%) and prominent catalytical activity (K_m, 0.18 mM; V_max, 10⁻⁸ M s⁻¹). In addition, based on the specifical bonding of PCN-224 towards antibiotic tetracycline (TC), we revealed that TC could inhibit the catalytical activity of PCN-224@Pt NPs nanozyme, and then a sensitive colorimetric sensing platform was thus designed to detect TC, which could effectively avoid the utilization of H₂O₂ coupled with superior analytical performances compared to the reported POD nanozyme-based sensing platforms. Meanwhile, by integrating this nanozyme to medical cotton swab and coupling with the help of smartphone, simple, low-cost and onsite analysis for TC could be achieved successfully. This work not only proposed an innovative way via defect engineering to design MOD nanozymes with excellent catalytical activity, but also developed a highly effective colorimetric and smartphone dual-mode sensing method for TC, thus showing great potential applications.

贵金属类氧化酶（MOD）纳米酶是一类常见的类过氧化物酶（POD）纳米酶，具有广泛的应用前景，但其活性和稳定性不足的问题亟待解决。本文通过引入缺陷卟啉Zr金属有机骨架（PCN-224）方作为金属载体，采用原位生长的方法，首次制备了一种以铂纳米粒子（PCN-224@Pt NPs）修饰的新型MOD纳米酶，并对其进行了表征，该酶具有极高的Pt负载量（72.82 wt%）和显著的催化活性（Km, 0.18 mM; Vmax, 10−8 M s−1）。此外，基于PCN-224对抗生素四环素（TC）的特异性结合，我们发现TC可以抑制PCN-224@Pt NPs纳米酶的催化活性，从而设计了一个灵敏的比色检测平台来检测TC，该平台可以有效地避免对h2o2的利用，并且与已有的基于POD纳米酶的检测平台相比，具有更好的分析性能。同时，将该纳米酶整合到医用棉签上，结合智能手机，可以成功实现简单、低成本的TC现场分析。本工作不仅提出了一种通过缺陷工程设计具有优异催化活性的MOD纳米酶的创新方法，而且还开发了一种高效的TC比色和智能手机双模传感方法，具有很大的应用潜力。

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引用次数: 0

Switchable hydrogen bond network regulates aggregation and dispersion of metal–organic frameworks for multimodal anticounterfeiting 可切换氢键网络调节多模态防伪金属有机框架的聚集和分散

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-07 DOI: 10.1016/j.snb.2026.139448

Li Yang , Zhaofeng Ran , Zhihui Xu , Weidong Zhao , Zhiyu Wang , Zilin Zhang , Yanjun Zhang , Yanzhen Yin , Wen Sun

A single-material system capable of exhibiting tunable multimodal luminescence integrates various luminescence modes, including fluorescence (FL) and chemiluminescence (CL), within a cohesive framework. This integration not only enhances the complexity and security of information encoding but also significantly improves the selectivity and sensitivity of sensing. Therefore, this system is especially well-suited for applications in sensing, anti-counterfeiting, and information encryption. However, achieving such versatility using a single-material system remains considerably challenging due to the potential incompatibility of different luminescence modes under external stimuli. In this study, we developed a dual-mode sensing method that enables the control of FL and CL through a dynamic hydrogen bond network. This method allows for the rapid detection of hydrogen peroxide (H₂O₂) in aqueous solutions and is applicable to anticounterfeiting applications. The synthesized UiO-66-(Zr)-NH₂ (Zr-MOF-NH₂) exhibits FL quenching due to energy transfer between NH₂-BDC ligands and Zr ions, resulting in negligible FL in aqueous solutions and in solid state. However, upon forming hydrogen bonds with H₂O₂, this energy transfer is suppressed, thereby activating the material’s luminescent response. This property enables Zr-MOF-NH₂ to selectively detect H₂O₂ with a linear FL response. In addition, aqueous solutions of Zr-MOF-NH₂ can serve as secure ink for printing encrypted information and patterns on paper. Notably, the encrypted information becomes visible only when exposed to specific external stimuli applied in the correct sequence, ensuring high anticounterfeiting security and selectivity. These results highlight the considerable potential of Zr-MOF-NH₂ for applications in information anticounterfeiting and encryption.

一个能够显示可调谐多模态发光的单材料系统集成了各种发光模式，包括荧光（FL）和化学发光（CL），在一个内聚框架内。这种集成不仅提高了信息编码的复杂性和安全性，而且显著提高了感知的选择性和灵敏度。因此，该系统特别适合于传感、防伪和信息加密方面的应用。然而，由于外部刺激下不同发光模式的潜在不兼容性，使用单一材料系统实现这种多功能性仍然具有相当大的挑战性。在这项研究中，我们开发了一种双模传感方法，可以通过动态氢键网络控制FL和CL。该方法可以快速检测水溶液中的过氧化氢（H2O2），适用于防伪应用。合成的uo2 -66-(Zr)- nh2 （Zr- mof - nh2）由于NH2-BDC配体和Zr离子之间的能量转移而发生FL猝灭，导致其在水溶液和固态中可忽略FL。然而，当与H2O2形成氢键时，这种能量传递被抑制，从而激活材料的发光响应。这种特性使Zr-MOF-NH2能够选择性地检测H2O2，并具有线性FL响应。此外，Zr-MOF-NH2水溶液可以作为安全油墨在纸上打印加密信息和图案。值得注意的是，加密的信息只有在暴露于以正确顺序施加的特定外部刺激时才可见，从而确保了高防伪安全性和选择性。这些结果突出了Zr-MOF-NH2在信息防伪和加密方面的巨大应用潜力。

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引用次数: 0

iSEA-in situ: A submersible flow-based nutrient sensor for autonomous measurement of ammonium in marine waters iSEA-in situ：一种可潜水的基于流量的营养传感器，用于自主测量海水中的铵

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-07 DOI: 10.1016/j.snb.2026.139441

Xiangyu Zhu , Ming Zhu , Tengyue Fang , Shikun Huang , Jianping Li , Jian Ma

Ammonium is a central component of the marine nitrogen cycle and a critical indicator of water quality. However, its susceptibility to environmental changes during sampling and analysis poses significant challenges for accurate measurement in marine waters. Here, we present iSEA-in situ, a submersible wet-chemistry sensor readily adapted from our previously developed programmable syringe pump-based flow analyzer. The sensor features a newly designed optical detection module, filtration module, and auto-calibration program tailored for in situ deployment. This sensor design offers a practical complement to microfluidics- and electrochemistry-based in situ sensors, circumventing the technical barrier of sophisticated microfabrication and sample matrix interference. Using a modified indophenol blue spectrophotometric method, the iSEA-in situ sensor demonstrates salinity-independent performance with high measurement frequency (6 h⁻¹), precision (relative standard deviation of 2.7% for underwater analysis of 5 μM standard solutions over 14 days), sensitivity (detection limit of 0.12 μM), wide dynamic range (up to 20 μM), and low reagent consumption (a total of 140 μL per run). Field deployment in a fishing tank and coral tank in a nearshore marine station yielded a total of 795 measurements with only 4.2% outliers (≥3σ residuals). Regular auto-calibration via measuring quality control samples confirmed sustained accuracy of the sensor (relative standard deviation ≤3.8%) over a week-long deployment. The iSEA-in situ sensor is capable of providing robust, high-temporal-resolution data to capture the dynamic interplay between marine biological activity and environmental fluctuations.

铵是海洋氮循环的核心组成部分，也是水质的重要指标。然而，在采样和分析过程中，其对环境变化的敏感性给海水的准确测量带来了重大挑战。在这里，我们展示了iSEA-in situ，这是一种潜水湿化学传感器，很容易适应于我们之前开发的基于可编程注射泵的流量分析仪。该传感器具有新设计的光学检测模块、过滤模块和为现场部署量身定制的自动校准程序。该传感器设计为基于微流体和电化学的原位传感器提供了实用的补充，绕过了复杂微加工和样品基质干扰的技术障碍。采用改进的吲哚酚蓝分光光度法，该传感器具有测量频率高（6 h−1）、精度高（对5个 μM标准溶液进行14天的水下分析，相对标准偏差为2.7%）、灵敏度高（检测限为0.12 μM）、动态范围宽（最高可达20 μM）、试剂消耗低（每次运行共140 μL）等特点。在近岸海洋站的渔池和珊瑚池中进行现场部署，共获得795个测量值，只有4.2%的异常值（残差≥3σ）。通过测量质量控制样本进行定期自动校准，确认传感器在长达一周的部署中持续保持精度（相对标准偏差≤3.8%）。isea原位传感器能够提供可靠的高时间分辨率数据，以捕获海洋生物活动与环境波动之间的动态相互作用。

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引用次数: 0

Wearable microfluidic chip with a highly selective lock-and-key fluorescent probe for rapid and noninvasive β-hydroxybutyrate diagnostics 可穿戴微流控芯片，具有高选择性锁键荧光探针，用于快速无创β-羟基丁酸盐诊断

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-07 DOI: 10.1016/j.snb.2026.139446

Qinglin Mo, Zhenlong Huang, Ziyi Luo, Fangrui Lin, Binjun Li, Hao Xu, Yiwen Sun, Jun Song, Xiaoyu Weng, Liwei Liu, Yu Chen, Junle Qu

β-Hydroxybutyrate (β-HB), a major physiological ketone body and key metabolic biomarker, is closely linked to metabolic health and disease management, yet highly sensitive, noninvasive analytical methods for β-HB detection via sweat remain scarce. Herein, we report a wearable microfluidic platform integrating a newly designed fluorescent probe (BHI) for rapid, sensitive and quantitative β-HB diagnostics in sweat. The BHI probe incorporates a thiourea donor group that forms specific hydrogen bonds with aldehyde or hydroxyl groups in β-HB, enabling a highly selective “lock-and-key” molecular recognition mechanism. This design delivers an ultralow detection limit (2.684 μM), a rapid response time (<5 s), and excellent selectivity against potential interferents. In ketogenic diet validation studies, the platform’s results showed a strong correlation (r = 0.99) with those obtained by the gold-standard clinical assay, confirming its quantitative accuracy. This wearable sensing strategy offers a practical, user-friendly tool for rapid and noninvasive assessment of ketosis, with potential applications in personalized metabolic health management.

β-羟基丁酸（β-HB）是一种主要的生理酮体和关键的代谢生物标志物，与代谢健康和疾病管理密切相关，但通过汗液检测β-HB的高灵敏度、无创分析方法仍然很少。在此，我们报道了一种可穿戴微流控平台，集成了一种新设计的荧光探针（BHI），用于快速、灵敏和定量地诊断汗液中的β-HB。BHI探针包含一个硫脲供体基团，可以与β-HB中的醛或羟基形成特定的氢键，从而实现高度选择性的“锁与钥匙”分子识别机制。该设计具有超低检测限（2.684 μM）、快速响应时间（<5 s）和对潜在干扰的优异选择性。在生酮饮食验证研究中，该平台的结果与金标准临床检测结果显示出很强的相关性（r = 0.99），证实了其定量准确性。这种可穿戴式传感策略为快速无创评估酮症提供了一种实用、用户友好的工具，在个性化代谢健康管理中具有潜在的应用前景。

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引用次数: 0

Protonation-driven nanobowl-confined dye assembly for boosting the ultrasensitive colorimetric immunochromatographic pathogen detection 质子驱动的纳米碗限制染料组件促进超灵敏比色免疫层析病原体检测

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-07 DOI: 10.1016/j.snb.2026.139443

Chenxin Ji , Zhaowen Cui , Chenjie Nie , Yuechun Li , Guo Hao , Yiya Ping , Bingzhi Li , Yuanyuan Cheng , Longhua Shi , Jianlong Wang , Leina Dou

Revealing the mechanism of dye molecules assembly within nanocavities and engineer corresponding ultrabright nanoassemblies represents a crucial advancement that not only pushes the boundaries of colorimetric immunochromatographic assay (ICA) technology, but also fundamentally enhances the detection performance of point-of-care diagnostics. Herein, protonation-powered m-aminophenol formaldehyde resin nanobowls-confined dye molecules assembly is proposed for the development of ultrabright nanoassemblies, which is used to enhance the purely colorimetric ICAs for the detection of pathogens in food-related matrices. The assembled process can be attributed to four synergistic effects: protonation-induced charge reversal, electrostatic-driven dye loading, concentration gradient-powered diffusion, and nanoconfinement and pore blocking, which significantly amplify the collective color signal per nanoparticle. When deployed as labels in immunochromatographic assays, these nanoassemblies enable naked-eye detection of Salmonella typhimurium at 500 CFU mL⁻¹, improving nearly 100-fold over conventional gold nanoparticle-based ICA strips. The method maintains excellent specificity and works in complex food matrices without instrumentation. This work establishes a general paradigm for engineering intense colorimetric signals through controlled molecular assembly in designed nanoreactors, bridging supramolecular chemistry with point-of-care diagnostics.

揭示染料分子在纳米腔内组装的机制并设计相应的超亮纳米组装是一项重要的进步，不仅推动了比色免疫层析分析（ICA）技术的发展，而且从根本上提高了即时诊断的检测性能。本文提出了质子动力间氨基酚甲醛树脂纳米碗限制染料分子组装，用于开发超亮纳米组装，用于增强纯比色ica，用于检测食品相关基质中的病原体。组装过程可归因于四种协同效应：质子诱导的电荷反转、静电驱动的染料负载、浓度梯度驱动的扩散、纳米限制和孔阻塞，这些协同效应显著放大了每个纳米颗粒的集体颜色信号。当作为免疫层析分析的标签时，这些纳米组件能够在500 CFU mL-1的浓度下裸眼检测鼠伤寒沙门氏菌，比传统的基于金纳米颗粒的ICA条提高近100倍。该方法保持了良好的特异性，并在复杂的食品基质中工作，无需仪器。这项工作建立了通过在设计的纳米反应器中控制分子组装来设计强比色信号的一般范例，将超分子化学与即时诊断联系起来。

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引用次数: 0

A handheld fully automated rotating magnetic field-driven integrated tube system with sample-in–answer-out capability 一种手持式全自动旋转磁场驱动集成管系统，具有样品输入应答能力

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-07 DOI: 10.1016/j.snb.2026.139442

Juxin Yin , Jizhi Tong , Guangye Shao , Jianda Shao , Mengda Liang , Wen Zong , Ningkang Xie , Yu Wang , Shaowu Lv , Ying Mu

Point-of-Care testing (POCT) has gained widespread attention for nucleic acid testing. However, existing magnetic bead-based POCT systems often face trade-offs between automation, structural complexity, and scalability, limiting portability and large-scale deployment. Here, this research proposed a magnetic bead-based all-in-one integrated tube driven by rotating magnetic field and developed a handheld and fully automated device for POCT. The tube performs sample lysis, purification, elution, PCR amplification, and detection in a compact and low-cost format. The handheld device controls magnetic fields, temperature, and fluorescence, enabling sample-in–answer-out within 1 h. In this way, HBV detection achieved high sensitivity (5 copies/μL), specificity, and a linear range of 5 × 10¹–5 × 10⁵ copies per reaction. This platform is portable, low-cost, and fully automated, offering a promising solution for POCT.

即时检测（POCT）在核酸检测中得到了广泛关注。然而，现有的基于磁珠的POCT系统经常面临自动化、结构复杂性和可扩展性之间的权衡，限制了可移植性和大规模部署。本研究提出了一种基于磁珠的旋转磁场驱动一体化集成管，开发了一种手持式全自动POCT装置。该管执行样品裂解，纯化，洗脱，PCR扩增，并在一个紧凑和低成本的格式检测。手持设备控制磁场，温度和荧光，使样品在1 h内输出。通过这种方法，HBV检测获得了高灵敏度（5个拷贝/μL）和特异性，并且每个反应的线性范围为5 × 101-5 × 10个拷贝。该平台具有便携、低成本和全自动化的特点，为POCT提供了一个很有前途的解决方案。

引用次数: 0

Field-ready detection of antineoplastic drugs in workplaces using a wearable flexible surface-enhanced Raman spectroscopy platform 使用可穿戴柔性表面增强拉曼光谱平台在工作场所现场检测抗肿瘤药物

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-07 DOI: 10.1016/j.snb.2026.139447

Xinying Ni , Hong Wang , Wenwen Chen , Xiao Meng , Mengping Zhang , Hua Shao , Fang Zhang , Cuijuan Wang

Occupational exposure to icotinib and gefitinib poses a significant health risk, underscoring the necessity for effective monitoring to safeguard environmental and occupational health. The present study introduces a wearable SERS platform designed for field-ready detection of antineoplastic drugs’ contamination. The SERS substrate can be assembled on gloves within medical settings exposed to antineoplastic drugs. HOF-101 nanorods coated with gold nanoflowers were loaded on a piece of cellulose fiber membrane (HOF-101@AuNFs/CF). Benefitting from the triple synergistic effects of efficient enrichment of porous HOF-101, abundant SERS hotspots of AuNFs, and adsorption of CF, a simple, field-ready sample-processing workflow and SERS detection of drug residues on workbench’s surface and pharmaceutical packaging was realized. This platform demonstrates a clear logarithmic correlation between SERS intensity and the concentration of icotinib and gefitinib within 10 ppb to 2 ppm, achieving satisfactory recovery and reproducibility. Rapid fingerprint identification and quantitative analysis of icotinib and gefitinib in different samples (workbench’s surface and pharmaceutical packaging) were achieved within 5 min, with the detection limits as low as 2.26–3.00 ppb. This in-field sampling and detecting method shows promise for mitigating risks from exposure to antineoplastic agents in the medical workplace.

职业接触伊可替尼和吉非替尼对健康构成重大风险，强调必须进行有效监测，以保障环境和职业健康。本研究介绍了一种用于现场检测抗肿瘤药物污染的可穿戴SERS平台。SERS底物可以在暴露于抗肿瘤药物的医疗环境中的手套上组装。将涂有金纳米花的HOF-101纳米棒装载在一片纤维素纤维膜上（HOF-101@AuNFs/CF）。利用多孔HOF-101的高效富集、aunf丰富的SERS热点和CF吸附的三重协同效应，实现了简单的现场样品处理工作流程，并对工作台表面和药品包装上的药物残留进行了SERS检测。该平台显示，在10 ppb ~ 2 ppm范围内，伊可替尼和吉非替尼的SERS强度与浓度之间存在明显的对数相关关系，具有满意的回收率和重现性。不同样品（工作台表面和药品包装）中伊可替尼和吉非替尼的快速指纹识别和定量分析在5 min内完成，检出限低至2.26 ~ 3.00 ppb。这种现场采样和检测方法有望减轻医疗工作场所暴露于抗肿瘤药物的风险。

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引用次数: 0

A colorimetric and surface-enhanced Raman scattering dual-readout lateral flow assay improves the accuracy of early diagnosis of Alzheimer's disease by simultaneously detecting Aβ42 oligomers and phosphorylated tau 一种比色和表面增强拉曼散射双读数横向流动试验通过同时检测Aβ42寡聚物和磷酸化tau提高了阿尔茨海默病早期诊断的准确性

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-06 DOI: 10.1016/j.snb.2025.139417

Liding Zhang , Xuewei Du , Taoping Zhang , Qingshi Chen , Qian Zhang , Ying Su , Min Zhang , Haiming Luo

Due to the low abundance of biomarkers, reliance on expensive instruments, and low reliability of single-biomarker detection, it is necessary to develop economical, accurate, and user-friendly blood-based Alzheimer's disease (AD) diagnostic methods. In this study, a highly sensitive colorimetric and surface-enhanced Raman scattering (SERS) dual-readout lateral flow immunoassay (LFIA) was developed to simultaneously detect two core AD biomarkers in plasma samples within 25 min, including Aβ₄₂ oligomers (Aβ₄₂O) and tau phosphorylated at Ser396 and Ser404 (p-tau^396,404). The assay leverages conformation-selective antibody pairs and high signal-to-noise ratio SERS nanotags to achieve femtomolar sensitivity, long signal lifetime, and strong anti-interference performance. Clinical evaluations demonstrated that the dual-target LFIA method effectively differentiated AD patients from cognitively normal elderly individuals, with a sensitivity and specificity of 86.7 % and an area under the receiver operating characteristic (ROC) curve (AUC) of 0.933, which is superior to the single-target assay. The economical and portable design of the dual-target LFIA enables rapid, accurate, and scalable detection, highlighting its potential as a practical tool for point-of-care diagnostics and large-scale community self-screening of AD.

由于生物标志物丰度低，依赖昂贵的仪器，单一生物标志物检测的可靠性低，有必要开发经济、准确、用户友好的基于血液的阿尔茨海默病（AD）诊断方法。在本研究中，开发了一种高灵敏度比色法和表面增强拉曼散射（SERS）双读侧流免疫分析法（LFIA），可在25 min内同时检测血浆样品中的两种核心AD生物标志物，包括a β42低聚物（a β 42o）和Ser396和Ser404磷酸化的tau （p-tau396,404）。该检测利用构象选择性抗体对和高信噪比的SERS纳米标签来实现飞摩尔灵敏度、长信号寿命和强抗干扰性能。临床评价表明，双靶点LFIA方法能有效区分AD患者与认知正常的老年人，灵敏度和特异度为86.7 %，受试者工作特征（ROC）曲线下面积（AUC）为0.933，优于单靶点法。经济、便携的双靶点LFIA设计能够实现快速、准确和可扩展的检测，突出了其作为即时诊断和大规模社区AD自我筛查的实用工具的潜力。

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引用次数: 0

Femtosecond laser micromachining triple-modality optical fiber sensor with multiband optical decoupling for simultaneous dual-biomarkers and temperature monitoring 飞秒激光微加工多波段光去耦三模光纤传感器，用于同时监测双生物标志物和温度

IF 3.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2026-01-06 DOI: 10.1016/j.snb.2026.139429

Pengqi Gong , Xuegang Li , Xue Zhou , Yanan Zhang , Tonglei Cheng , Linh Viet Nguyen , Stephen C. Warren-Smith , Yong Zhao

To address the insufficient information from single-parameter biological detection, this study proposes a multi-parameter optical biosensing scheme based on femtosecond laser micromachining (FLM), enabling simultaneous detection of creatinine, urea and temperature monitoring. Without damaging the main structure of the optical fiber, FLM modifies its internal structure to achieve precise waveguide fabrication, refractive index (RI) regulation and cladding mode coupling. On this basis, Mach-Zehnder interferometry (MZI), surface plasmon resonance (SPR, for biological signal detection) and fiber Bragg grating (FBG, for temperature detection) are integrated into a single microstructured optical fiber (MOF). The system is analyzed via detailed theoretical simulation: the RI sensitivities of SPR and MZI are 1842 nm/RIU and 516 nm/RIU, respectively, while their temperature sensitivities (along with FBG) are −0.242 nm/℃, −0.054 nm/℃ and 0.012 nm/℃. By combining specific sensitive materials with microfluidic chip channels, an MZI urea detection channel, an SPR creatinine detection channel and an FBG temperature monitoring mechanism are constructed. Experimental results show the structure can monitor temperature and has good selective detection capability for creatinine and urea, with sensitivities of 7.2 nm/mM and 3.8 nm/mM, and limits of detection (LOD) of 0.07 mM and 0.09 mM, confirming its excellent dual biological indicator detection performance.

为了解决单参数生物检测信息不足的问题，本研究提出了一种基于飞秒激光微加工（FLM）的多参数光学生物传感方案，可以同时检测肌酐、尿素和温度监测。在不破坏光纤主体结构的前提下，通过改变光纤内部结构，实现波导的精密制造、折射率（RI）调节和包层模式耦合。在此基础上，将Mach-Zehnder干涉测量（MZI）、表面等离子体共振（SPR，用于生物信号检测）和光纤布拉格光栅（FBG，用于温度检测）集成到单个微结构光纤（MOF）中。通过详细的理论模拟对系统进行了分析：SPR和MZI的RI灵敏度分别为1842 nm/RIU和516 nm/RIU，温度灵敏度（与FBG一起）分别为- 0.242 nm/℃、- 0.054 nm/℃和0.012 nm/℃。将特定敏感材料与微流控芯片通道相结合，构建了MZI尿素检测通道、SPR肌酐检测通道和FBG温度监测机制。实验结果表明，该结构具有良好的温度监测功能，对肌酐和尿素具有良好的选择性检测能力，灵敏度分别为7.2 nm/mM和3.8 nm/mM，检出限（LOD）分别为0.07 mM和0.09 mM，证实了其良好的双生物指标检测性能。

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