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MVSP-SACFormer: A mixed gas identification method under low- resource conditions MVSP-SACFormer:一种低资源条件下的混合气体识别方法
IF 3.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2026-01-26 DOI: 10.1016/j.snb.2026.139554
Renwei Guan , Kailin Zhang , Hanzhe Liu , Jianbo Li , Ziwen Wang , Xinxin Sun , Jingli Feng , Yan Li , Xuyang Zhu , Ju Zhang
Inspired by the mammalian olfactory system, electronic nose (e-nose) technology has been widely applied in environmental monitoring, food safety, and medical diagnostics. However, mixed gas identification still faces two challenges. Firstly, the gas sensor array exhibits significant cross-sensitivity and low selectivity, resulting in highly overlapped responses among different gases, with redundant features masking key discriminative information. This leads to a sparse and undefined decision space. Secondly, in practical applications, e-nose systems often suffer from limited training samples, making existing methods unstable under low-resource conditions. To solve these problems, this paper proposes MVSP-SACFormer, a mixed gas identification framework tailored for low-resource situations. In the feature extraction phase, we design a Multi-View Subspace Projection Encoding (MVSP) mechanism, which maps raw sensor responses into multiple low-dimensional subspaces via independent random observation matrices, generating compressed features that are structurally diverse and information-complementary. This enhances feature expressiveness under low resource. In the modeling phase, we construct a Spectral-Aware Convolutional Transformer (SACFormer) that fuses spectral, dynamic temporal, and spatial structural features to deeply model the key patterns within complex mixed gas responses. Experiments on two public mixed gas datasets demonstrate that MVSP-SACFormer achieves outstanding recognition performance even when using only 40 % of the training data: achieving 98.03 % classification accuracy on a methane–ethylene mixture dataset, and 97.06 % on an ethylene–carbon monoxide mixture dataset. These results validate that the proposed method serves as a universal pattern recognition framework for e-nose systems under low-resource conditions.
受哺乳动物嗅觉系统的启发,电子鼻技术在环境监测、食品安全、医疗诊断等领域得到了广泛的应用。然而,混合气的识别仍然面临着两个挑战。首先,气体传感器阵列具有显著的交叉灵敏度和低选择性,导致不同气体之间的响应高度重叠,冗余特征掩盖了关键的判别信息。这将导致稀疏且未定义的决策空间。其次,在实际应用中,电子鼻系统经常受到训练样本有限的影响,使得现有方法在低资源条件下不稳定。为了解决这些问题,本文提出了MVSP-SACFormer,这是一种针对低资源情况量身定制的混合气体识别框架。在特征提取阶段,我们设计了一种多视图子空间投影编码(MVSP)机制,该机制通过独立的随机观测矩阵将原始传感器响应映射到多个低维子空间中,生成结构多样且信息互补的压缩特征。这增强了低资源下的特性表现力。在建模阶段,我们构建了一个光谱感知卷积变压器(SACFormer),它融合了光谱、动态时间和空间结构特征,以深入模拟复杂混合气体响应中的关键模式。在两个公共混合气体数据集上的实验表明,即使只使用40%的训练数据,MVSP-SACFormer也取得了出色的识别性能:在甲烷-乙烯混合物数据集上实现了98.03%的分类准确率,在乙烯-一氧化碳混合物数据集上实现了97.06%的分类准确率。结果表明,该方法可作为低资源条件下电子鼻系统的通用模式识别框架。
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引用次数: 0
A cyclic feedback DNA circuit for high-performance detection of aflatoxin B1 用于黄曲霉毒素B1高效检测的循环反馈DNA电路
IF 3.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2026-01-25 DOI: 10.1016/j.snb.2026.139543
Zhigang Li, Xiaojiao Dai, Ran Li, Yi Xu, Qingmin Chen, Xiaomei Chen, Quansheng Chen, Jie Wei
Isothermal DNA amplifiers have emerged as powerful toolkits for the precise determination of contaminants in complex food matrices. Nevertheless, current DNA circuits suffer from the limitation of low amplification capacity. Here a cyclic feedback amplification (CFA) system with exponential signal gain was proposed for aflatoxin B1 (AFB1) analysis through synergistic cross-activation of catalytic hairpin assembly (CHA) and exonuclease III (Exo III)-powered amplification. The initiator activates CHA to generate numerous fuel strands. These fuels then initiate Exo III-powered amplification to generate numerous initiators that feed back into CHA, undergoing a mutually reinforcing cyclic amplification process. The CFA system realizes successive regeneration of the initiator and fuel strands, achieving exponential signal amplification for highly sensitive analyte detection. The CFA system, as a versatile and robust amplification tool, enables reliable detection of AFB1 in buffer, wheat, and maize samples by introducing an auxiliary recognition element, offering great potential for trace food contaminant analysis.
等温DNA放大器已经成为精确测定复杂食物基质中污染物的强大工具。然而,目前的DNA电路受到低扩增能力的限制。本文提出了一种具有指数信号增益的循环反馈扩增(CFA)系统,通过催化发夹组装(CHA)和外切酶III (Exo III)的协同交叉激活放大,用于黄曲霉毒素B1 (AFB1)的分析。发起者激活CHA产生大量的燃料链。然后,这些燃料启动Exo iii驱动的放大,产生大量的启动物,这些启动物反馈给CHA,经历一个相互加强的循环放大过程。CFA系统实现了引发剂和燃料链的连续再生,实现了高灵敏度分析物检测的指数级信号放大。CFA系统作为一种多功能和强大的扩增工具,通过引入辅助识别元件,可以可靠地检测缓冲液,小麦和玉米样品中的AFB1,为痕量食品污染物分析提供了巨大的潜力。
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引用次数: 0
A chemiluminescent system for in vivo tumor imaging via the simultaneous response of H2O2 and pH under different wavelength 一种利用H2O2和pH在不同波长下同时响应的体内肿瘤化学发光系统
IF 3.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2026-01-24 DOI: 10.1016/j.snb.2026.139548
Qihang Sun , Jingyu Huang , Lixia Bai , Yu Zhou , Wen Wu , Xiaoyang Chen , Yawen Hong , Shangjun Jiang , Shaoqing Zhuang , Chunya Li , Zhong-Hua Ma
Chemiluminescence (CL), an optical technique requiring no external excitation source, has become an efficient tool in bioimaging. However, traditional CL imaging systems are predominantly constrained by the use of hydrogen peroxide (H2O2) as both the coreactant and the primary recognition target, which limits their versatility and range of applications. To overcome this limitation, we present a novel chemiluminescent imaging system that integrates a near-infrared (NIR)-emitting pH probe as a secondary emitter alongside H2O2 recognition, facilitating the simultaneous response of H2O2 and pH value. This dual-emitter system was rigorously validated in a murine tumor model, demonstrating successful dual-signal in vivo imaging at distinct emission wavelengths of 540 nm (H2O2) and 840 nm (pH). Under the characteristic of a tumor microenvironment (elevated H2O2 levels and slightly acidic pH), a significant enhancement in signal-to-noise ratio was observed with the CL system, showing increases of 18.57-fold and 11.67-fold over fluorescence imaging at 540 nm and 840 nm, thus offering significant improvements in sensitivity, specificity and resolution.
化学发光技术作为一种不需要外界激励的光学技术,已成为生物成像的有效工具。然而,传统的CL成像系统主要受到过氧化氢(H2O2)作为共反应物和主要识别目标的限制,这限制了它们的通用性和应用范围。为了克服这一限制,我们提出了一种新的化学发光成像系统,该系统将近红外(NIR)发射pH探针作为辅助发射器与H2O2识别集成在一起,促进H2O2和pH值的同时响应。该双发射器系统在小鼠肿瘤模型中得到了严格的验证,在540nm (H2O2)和840nm (pH)的不同发射波长下成功实现了双信号的体内成像。在肿瘤微环境(H2O2水平升高,pH值微酸性)的特点下,CL系统的信噪比明显增强,比荧光成像在540nm和840nm分别提高了18.57倍和11.67倍,灵敏度、特异性和分辨率均有显著提高。
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引用次数: 0
From raw signals to reliable electrochemical sensing: Data preprocessing strategies for machine learning supported energetic compound identification 从原始信号到可靠的电化学传感:机器学习支持的高能化合物识别的数据预处理策略
IF 3.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2026-01-24 DOI: 10.1016/j.snb.2026.139542
Daan Vangerven , Julia Mazurków , Bart Simoens , Karolien De Wael
The widespread use of energetic compounds in armed conflicts, terrorism, and criminal activities highlights the need for rapid, accurate, and field-deployable detection and identification methods. Electrochemical sensing offers a promising solution, as many energetic compounds contain nitro groups that undergo electrochemical reduction, generating compound-specific electrochemical fingerprints. However, overlapping signals and concentration variability complicate robust identification. In this study, we present a Random Forest-based machine learning algorithm to identify ten nitro-containing energetic compounds and one binary mixture using square wave voltammetry. Voltammetric responses were collected over a concentration range of 50 – 200 µg/mL using bare in-house screen-printed electrodes. Six Random Forest models were developed based on different input data: (1) extracted peak parameters, (2) raw voltammetric data, and (3) Discrete Wavelet Transform (DWT)-processed data. Models trained on raw voltammetric and DWT-processed data using default hyperparameters achieved the highest overall classification accuracy in tests with samples representative for real-life scenarios. Confidence scores enabled quantitative evaluation of model predictions, with the raw voltammetric data model delivering the most confident outcomes. This study demonstrates a novel concentration-independent, machine learning-based electrochemical strategy for the accurate energetic compound identification in field applications.
高能化合物在武装冲突、恐怖主义和犯罪活动中的广泛使用凸显了对快速、准确和可实地部署的探测和识别方法的需求。电化学传感提供了一个很有前途的解决方案,因为许多含能化合物含有硝基,经过电化学还原,产生化合物特定的电化学指纹。然而,重叠信号和浓度变异性使鲁棒性识别复杂化。在这项研究中,我们提出了一种基于随机森林的机器学习算法,使用方波伏安法识别十种含硝基含能化合物和一种二元混合物。在50 - 200 µg/mL的浓度范围内,使用裸露的内部丝网印刷电极收集伏安响应。基于不同的输入数据建立了6个随机森林模型:(1)提取的峰值参数,(2)原始伏安数据,(3)离散小波变换(DWT)处理的数据。使用默认超参数对原始伏安和dwt处理的数据进行训练的模型在具有实际场景代表性的样本的测试中获得了最高的总体分类精度。置信度评分能够对模型预测进行定量评估,原始伏安数据模型提供了最可靠的结果。本研究展示了一种新的、与浓度无关的、基于机器学习的电化学策略,用于在现场应用中准确识别含能化合物。
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引用次数: 0
One-step construction of portable CuNCs hydrogel probes for multicolor visual detection of norfloxacin 一步法构建便携式cncs水凝胶多色肉眼检测诺氟沙星
IF 3.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2026-01-24 DOI: 10.1016/j.snb.2026.139547
Junjie Guo , Mengxi Miao , Ninghan Tang , Xiandeng Hou , Shan Pu , Lan Wu
In this work, we reported a simple one-pot strategy to construct a fluorescent hydrogel probe of Tb-CuNCs-SA based on glutathione-modified copper nanoclusters (CuNCs), sodium alginate (SA), and Tb3 + ions. Tb3+ played multiple roles including a crosslinker to form a 3D hydrogel network, inducing aggregation-induced red emission of CuNCs, and specifically coordinating with NOR to emit characteristic green fluorescence through the “antenna effect”. The results showed that this probe could be used for multicolor visual detection of NOR. As NOR concentration increased, the hydrogel fluorescence changed from orange-red to yellow to green, enabling intuitive visual discrimination. By extracting RGB values using a smartphone application, a linear relationship between G/R ratio and NOR concentration was established, achieving a limit of detection as low as 7.1 nM. This value was much lower than the maximum residue limits for fluoroquinolone antibiotics in food of animal origin set by both European Union and China. Furthermore, the probe demonstrated excellent stability under harsh conditions and allowed rapid, pretreatment-free NOR detection in milk, honey, and serum within 30 min. This work provides a facile and sensitive platform for on-site antibiotic monitoring, offering great potential for practical environmental and food safety analysis.
在这项工作中,我们报道了一种简单的单锅策略,以谷胱甘肽修饰的铜纳米团簇(CuNCs)、海藻酸钠(SA)和Tb3+离子为基础构建了tb - cccs -SA荧光水凝胶探针。Tb3+发挥了多种作用,包括作为交联剂形成三维水凝胶网络,诱导聚集体诱导的ccc红色发射,以及通过“天线效应”特异性地与NOR协同发出特征绿色荧光。结果表明,该探针可用于NOR的多色视觉检测。随着NOR浓度的增加,水凝胶荧光由橙红色变为黄色再变为绿色,可以进行直观的视觉辨别。通过智能手机应用程序提取RGB值,建立了G/R比与NOR浓度之间的线性关系,实现了低至7.1 nM的检测限。这一数值远低于欧盟和中国规定的动物源性食品中氟喹诺酮类抗生素的最大残留限量。此外,该探针在恶劣条件下表现出优异的稳定性,可以在30分钟内快速,无需预处理地检测牛奶,蜂蜜和血清中的NOR。这项工作为抗生素现场监测提供了一个方便、灵敏的平台,为实际环境和食品安全分析提供了巨大的潜力。
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引用次数: 0
Dual-beam re-injection OA-ICOS for high sensitivity oxygen detection at 760 nm 双光束回注OA-ICOS用于760 nm高灵敏度氧检测
IF 3.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2026-01-24 DOI: 10.1016/j.snb.2026.139506
Hongzhe Lu , Qixin He , Zhangtai Chen , Qinghua Song , Jiakun Li , Qibo Feng
Re-injection off-axis integrated cavity output spectroscopy (RE-OA-ICOS) significantly enhances the signal power efficiency of the conventional OA-ICOS by incorporating a re-injection mirror in front of the cavity. However, the dense spatial overlap of re-injected beams leads to strong interference, which reduces the signal quality. We proposed a novel dual-beam re-injection scheme to realize the synergy of optical power enhancement and noise suppression through a dual-aperture re-injection mirror. By optimizing the injection position of the dual beams, spatially separated and more evenly distributed light spots are formed on the cavity mirror. This configuration not only further enhances the cavity mode density and mitigates cavity mode noise but also more effectively averages out spatially correlated noise. Comparative experiments show that the dual-beam re-injection approach achieves a detection limit of 89 ppm at 1 s integration time, which is improved by a factor of 1.5 compared to the single-beam re-injection approach. This work provides a new off-axis integrating cavity re-injection scheme, which is of great significance for the development of OA-ICOS sensors with higher signal-to-noise ratio and sensitivity.
再注入离轴集成腔输出光谱(RE-OA-ICOS)通过在腔前加入一个再注入反射镜,显著提高了传统的OA-ICOS的信号功率效率。然而,重注入波束的密集空间重叠导致了强烈的干扰,从而降低了信号质量。提出了一种新的双光束回射方案,通过双孔径回射镜实现光功率增强和噪声抑制的协同作用。通过优化双光束注入位置,在腔镜上形成空间分离且分布更均匀的光斑。这种结构不仅进一步提高了腔模密度,减轻了腔模噪声,而且更有效地平均了空间相关噪声。对比实验表明,双光束回注方法在1 s积分时间内的检出限为89 ppm,比单光束回注方法提高了1.5倍。该工作提供了一种新的离轴积分腔回注方案,对开发具有更高信噪比和灵敏度的OA-ICOS传感器具有重要意义。
{"title":"Dual-beam re-injection OA-ICOS for high sensitivity oxygen detection at 760 nm","authors":"Hongzhe Lu ,&nbsp;Qixin He ,&nbsp;Zhangtai Chen ,&nbsp;Qinghua Song ,&nbsp;Jiakun Li ,&nbsp;Qibo Feng","doi":"10.1016/j.snb.2026.139506","DOIUrl":"10.1016/j.snb.2026.139506","url":null,"abstract":"<div><div>Re-injection off-axis integrated cavity output spectroscopy (RE-OA-ICOS) significantly enhances the signal power efficiency of the conventional OA-ICOS by incorporating a re-injection mirror in front of the cavity. However, the dense spatial overlap of re-injected beams leads to strong interference, which reduces the signal quality. We proposed a novel dual-beam re-injection scheme to realize the synergy of optical power enhancement and noise suppression through a dual-aperture re-injection mirror. By optimizing the injection position of the dual beams, spatially separated and more evenly distributed light spots are formed on the cavity mirror. This configuration not only further enhances the cavity mode density and mitigates cavity mode noise but also more effectively averages out spatially correlated noise. Comparative experiments show that the dual-beam re-injection approach achieves a detection limit of 89 ppm at 1 s integration time, which is improved by a factor of 1.5 compared to the single-beam re-injection approach. This work provides a new off-axis integrating cavity re-injection scheme, which is of great significance for the development of OA-ICOS sensors with higher signal-to-noise ratio and sensitivity.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"453 ","pages":"Article 139506"},"PeriodicalIF":3.7,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vertically aligned CuO nanowire arrays on silicon substrates for high-performance hydrogen sensing 硅衬底上垂直排列的CuO纳米线阵列用于高性能氢传感
IF 3.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2026-01-24 DOI: 10.1016/j.snb.2026.139519
Mingzeng Sheng, Haibin Ni, Jiasheng Han, Sheng Ye, Zichen Chen, Yajie Wang, Bo Ni, Jianhua Chang
This work reports a CMOS-compatible and controllable route for fabricating vertically aligned CuO nanowire (NW) arrays directly on silicon substrates via AAO-assisted electrodeposition, followed by wet etching and annealing. The resulting monoclinic CuO NW arrays exhibit highly ordered alignment, uniform morphology, and enlarged active surface area, which collectively enhance gas diffusion and charge transport. The hydrogen sensor based on this architecture demonstrates an outstanding response of 625 % to 1 v/v% H2 at 200°C, with rapid response/recovery times of 30 s and 52 s, respectively. The device also maintains stable performance over 10 cycles and four weeks, confirming long-term durability. Owing to its wafer-level compatibility, low power consumption, and superior response, this approach provides a scalable pathway for integrating high-performance CuO-based hydrogen microsensors into modern microelectronic and industrial safety systems.
这项工作报告了一种cmos兼容和可控的方法,通过aao辅助电沉积,然后湿法蚀刻和退火,直接在硅衬底上制造垂直排列的CuO纳米线(NW)阵列。由此得到的单斜CuO NW阵列具有高度有序的排列,均匀的形貌和扩大的活性表面积,共同增强了气体扩散和电荷输运。基于该结构的氢气传感器在200°C下对1 v/v% H2的响应率为625%,快速响应/恢复时间分别为30秒和52秒。该设备在10个周期和4周内保持稳定的性能,证实了长期耐用性。由于其晶圆级兼容性,低功耗和卓越的响应,该方法为将高性能铜基氢微传感器集成到现代微电子和工业安全系统中提供了可扩展的途径。
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引用次数: 0
Catalytic hairpin assembly-driven DNAzyme for sensitive point-of-care colorimetric detection of Escherichia coli O157:H7 催化发夹组件驱动的DNAzyme用于敏感的护理点比色法检测大肠杆菌O157:H7
IF 3.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2026-01-24 DOI: 10.1016/j.snb.2026.139498
Xinli Shi , Yan Zhang , Yujun Sun , Chunping Ge , Rui Zhang , Ruobing Jia , Ziyu Liu , Shusheng Zhang , Xinyue Song
Foodborne pathogens such as Escherichia coli O157:H7 (E. coli O157:H7) pose a serious global health threat, thus, the high cost and operational complexity of the conventional detection methods have driven the development of rapid, sensitive, and portable diagnostic platforms. In this study, we developed a colorimetric biosensor for the rapid detection of E. coli O157:H7. The biosensor utilized a dual-aptamers recognition probe that specifically bound to E. coli O157:H7, triggering a non-enzyme signal amplification strategy that generated G-quadruplex (G4) fragments. These G4 fragments then facilitated the formation of a DNAzyme complex in the presence of Hemin which exhibited horseradish peroxidase (HRP)-mimicking activity, catalyzing the oxidation of a chromogen substrate and producing a visible colorimetric signal. The assay demonstrated a broad dynamic range from 2 to 107 CFU/mL with an ultra low limit of detection (LOD) of 11 CFU in 10 mL, ensuring highly sensitive and selective detection, even in complex sample matrices. Notably, the colorimetric signal could be easily analyzed using a smartphone, displaying excellent linearity and enabling point-of-care testing (POCT). Overall, the developed assay demonstrated high specificity, cost-effectiveness, and portability, making it a promising tool for on-site food safety monitoring and public health surveillance.
大肠杆菌O157:H7 (E. coli O157:H7)等食源性病原体对全球健康构成严重威胁,因此,传统检测方法的高成本和操作复杂性推动了快速、敏感和便携式诊断平台的发展。在本研究中,我们开发了一种快速检测大肠杆菌O157:H7的比色生物传感器。该生物传感器利用双适体识别探针特异性结合大肠杆菌O157:H7,触发非酶信号扩增策略,产生g -四重体(G4)片段。这些G4片段随后在Hemin存在的情况下促进了DNAzyme复合物的形成,该复合物表现出辣根过氧化物酶(HRP)模拟活性,催化显色底物的氧化并产生可见的比色信号。该分析具有2 ~ 107 CFU/mL的宽动态范围,超低检出限(LOD)为11cfu / 10mL,即使在复杂的样品基质中也能确保高灵敏度和选择性检测。值得注意的是,比色信号可以使用智能手机轻松分析,显示出色的线性并实现点护理测试(POCT)。总的来说,开发的检测方法具有高特异性、成本效益和便携性,使其成为现场食品安全监测和公共卫生监测的有前途的工具。
{"title":"Catalytic hairpin assembly-driven DNAzyme for sensitive point-of-care colorimetric detection of Escherichia coli O157:H7","authors":"Xinli Shi ,&nbsp;Yan Zhang ,&nbsp;Yujun Sun ,&nbsp;Chunping Ge ,&nbsp;Rui Zhang ,&nbsp;Ruobing Jia ,&nbsp;Ziyu Liu ,&nbsp;Shusheng Zhang ,&nbsp;Xinyue Song","doi":"10.1016/j.snb.2026.139498","DOIUrl":"10.1016/j.snb.2026.139498","url":null,"abstract":"<div><div>Foodborne pathogens such as <em>Escherichia coli</em> O157:H7 (<em>E. coli</em> O157:H7) pose a serious global health threat, thus, the high cost and operational complexity of the conventional detection methods have driven the development of rapid, sensitive, and portable diagnostic platforms. In this study, we developed a colorimetric biosensor for the rapid detection of <em>E. coli</em> O157:H7. The biosensor utilized a dual-aptamers recognition probe that specifically bound to <em>E. coli</em> O157:H7, triggering a non-enzyme signal amplification strategy that generated G-quadruplex (G4) fragments. These G4 fragments then facilitated the formation of a DNAzyme complex in the presence of Hemin which exhibited horseradish peroxidase (HRP)-mimicking activity, catalyzing the oxidation of a chromogen substrate and producing a visible colorimetric signal. The assay demonstrated a broad dynamic range from 2 to 10<sup>7</sup> CFU/mL with an ultra low limit of detection (LOD) of 11 CFU in 10 mL, ensuring highly sensitive and selective detection, even in complex sample matrices. Notably, the colorimetric signal could be easily analyzed using a smartphone, displaying excellent linearity and enabling point-of-care testing (POCT). Overall, the developed assay demonstrated high specificity, cost-effectiveness, and portability, making it a promising tool for on-site food safety monitoring and public health surveillance.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"453 ","pages":"Article 139498"},"PeriodicalIF":3.7,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146047807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Field-deployable PAM/PFS-independent RspCas13d platform for rapid and extraction-free detection of ASFV and its gene-deleted strains 可现场部署的不依赖于PAM/ pfs的RspCas13d平台,用于快速和无提取检测ASFV及其基因缺失菌株
IF 3.7 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2026-01-24 DOI: 10.1016/j.snb.2026.139544
Tong Xu , Yangming Dai , Lei Zhao , Dishi Chen , Si-yuan Lai , Ling Zhu , Zhi-wen Xu
ASFV causes high mortality and economic losses, and the emergence of gene-deleted strains highlights the need for rapid, sensitive, field-deployable diagnostics. Here, we present an integrated diagnostic workflow termed LUCID–FLASH, which combines a rapid extraction-free lysis protocol (LUCID, Lysis Using Chemical Integration for Direct detection) with a single-step RAA–CRISPR/RspCas13d assay (FLASH, FLASH-deployable Lyophilized All-in-one Single-tube Hybrid-detection). Together, these modules enable direct, instrument-free, and visual ASFV detection from swab samples within 30 min. We optimized and, for the first time, defined a chemical buffer formulation that supports high activities of both RAA and RspCas13d in a unified reaction. Meanwhile, LUCID facilitates room-temperature nucleic acid release without extraction kits or heating, ensuring full compatibility with FLASH for POCT. The PAM- and PFS-independent nature of RspCas13d enables flexible and unconstrained target design, enhancing assay versatility. Lyophilized reagents enhance stability and enable long-term storage and transportation. The LUCID–FLASH system supports dual instrument-free visual readouts (fluorescence and lateral flow assay), simplifying field deployment. Clinical validation demonstrated 100 % sensitivity and specificity relative to qPCR, and the system reliably differentiated ASFV wild-type and gene-deleted strains. Furthermore, its robust performance under both physiological (37 °C) and ambient (25 °C) conditions eliminates dependence on temperature-controlled equipment.
非洲猪瘟造成高死亡率和经济损失,基因缺失毒株的出现突出表明需要快速、敏感、可在现场部署的诊断方法。在这里,我们提出了一个称为LUCID - FLASH的集成诊断工作流程,它结合了快速无提取裂解协议(LUCID, lysis Using Chemical Integration for Direct detection)和单步RAA-CRISPR /RspCas13d测定(FLASH,可部署FLASH的冻干All-in-one单管混合检测)。总之,这些模块可以在30分钟内从拭子样本中直接、无仪器、直观地检测ASFV。我们优化并首次定义了一种化学缓冲配方,该配方在统一反应中支持RAA和RspCas13d的高活性。同时,LUCID无需提取试剂盒或加热即可实现室温核酸释放,确保与FLASH完全兼容。RspCas13d与PAM和pfs无关的特性使其能够灵活和不受约束地设计靶标,从而增强了检测的通用性。冻干试剂增强稳定性,便于长期储存和运输。LUCID-FLASH系统支持双仪器可视化读数(荧光和横向流分析),简化了现场部署。临床验证表明,该系统相对于qPCR具有100%的敏感性和特异性,能够可靠地区分ASFV野生型和基因缺失型菌株。此外,它在生理(37°C)和环境(25°C)条件下的强大性能消除了对温控设备的依赖。
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引用次数: 0
LAMFIA: A Modular, Lego®-like Assembled Plastic Microfluidic Platform for Advanced Lateral Flow Immunoassays LAMFIA:模块化,乐高®样组装塑料微流控平台,用于先进的横向流动免疫分析
IF 8.4 1区 化学 Q1 CHEMISTRY, ANALYTICAL Pub Date : 2026-01-24 DOI: 10.1016/j.snb.2026.139546
Juyeong Kim, Wonhyung Lee, Hye Jin An, Hojin Kim
The COVID-19 pandemic has underscored the need for rapid and reliable platforms capable of quantitative antibody detection. Lateral flow immunoassays (LFIAs) are widely adopted because of their simplicity and low cost. However, they typically lack the sensitivity and quantitative accuracy necessary for reliable clinical decision-making. In this paper, we present the Lego®-like assembled microfluidic flow immunoassay (LAMFIA), a modular and scalable plastic LFIA platform for quantitatively detecting anti–SARS-CoV-2 IgG antibodies. LAMFIA uses an open-channel architecture with bonding-free assembly, enabling spontaneous capillary flow, accurate fluid handling, and versatile bead-based assay integration. The system achieves excellent linearity across a clinically relevant concentration range (7.8–1000µg/mL) within 15min. Additionally, we present an in-flow signal amplification strategy that reduces the limit of detection from ~980pg/mL to <10pg/mL without extending assay time. By integrating all reagents into a single, hands-free workflow, LAMFIA offers a rapid, sensitive, and scalable solution for next-generation point-of-care diagnostics.
2019冠状病毒病大流行凸显了对能够定量检测抗体的快速可靠平台的需求。侧流免疫分析法(LFIAs)因其简单、成本低而被广泛采用。然而,它们通常缺乏可靠的临床决策所需的敏感性和定量准确性。在本文中,我们提出了Lego®样组装微流控流免疫分析(LAMFIA),这是一种模块化和可扩展的塑料LFIA平台,用于定量检测抗sars - cov -2 IgG抗体。LAMFIA采用开放通道架构,无粘合组装,实现自发毛细管流动,精确的流体处理和多功能的基于珠的分析集成。该系统在15分钟内实现了临床相关浓度范围(7.8-1000µg/mL)的良好线性。此外,我们提出了一种流动信号放大策略,在不延长检测时间的情况下,将检测限从~980pg/mL降低到<;10pg/mL。通过将所有试剂集成到一个单一的、免提的工作流程中,LAMFIA为下一代即时诊断提供了快速、敏感和可扩展的解决方案。
{"title":"LAMFIA: A Modular, Lego®-like Assembled Plastic Microfluidic Platform for Advanced Lateral Flow Immunoassays","authors":"Juyeong Kim, Wonhyung Lee, Hye Jin An, Hojin Kim","doi":"10.1016/j.snb.2026.139546","DOIUrl":"https://doi.org/10.1016/j.snb.2026.139546","url":null,"abstract":"The COVID-19 pandemic has underscored the need for rapid and reliable platforms capable of quantitative antibody detection. Lateral flow immunoassays (LFIAs) are widely adopted because of their simplicity and low cost. However, they typically lack the sensitivity and quantitative accuracy necessary for reliable clinical decision-making. In this paper, we present the Lego®-like assembled microfluidic flow immunoassay (LAMFIA), a modular and scalable plastic LFIA platform for quantitatively detecting anti–SARS-CoV-2 IgG antibodies. LAMFIA uses an open-channel architecture with bonding-free assembly, enabling spontaneous capillary flow, accurate fluid handling, and versatile bead-based assay integration. The system achieves excellent linearity across a clinically relevant concentration range (7.8–1000<ce:hsp sp=\"0.25\"></ce:hsp>µg/mL) within 15<ce:hsp sp=\"0.25\"></ce:hsp>min. Additionally, we present an in-flow signal amplification strategy that reduces the limit of detection from ~980<ce:hsp sp=\"0.25\"></ce:hsp>pg/mL to &lt;10<ce:hsp sp=\"0.25\"></ce:hsp>pg/mL without extending assay time. By integrating all reagents into a single, hands-free workflow, LAMFIA offers a rapid, sensitive, and scalable solution for next-generation point-of-care diagnostics.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"100 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2026-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146047805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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