Sensors and Actuators B: Chemical最新文献_第9页

Millimeter-wave measurements in high finesse cavity of nitro-derivatives traces: A new insight in the explosive vapor sensing

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-23 DOI: 10.1016/j.snb.2025.137629

Mhamad Chrayteh , Fabien Simon , Francis Hindle , Gaël Mouret , Anthony Roucou , Manuel Goubet , Julien Mory , Christelle Nicollet , Arnaud Cuisset

Cavity-Enhanced Absorption Spectroscopy (CEAS) and Cavity Ring-Down Spectroscopy (CRDS) are well established for sensitive infrared measurements of gas-phase compounds at trace levels using their rovibrational signatures. The recent successful development of a THz Fabry–Perot spectrometer by Hindle et al. (2019) shows that the adaptation of such techniques to submillimeter wavelengths allows to probe rotational transitions of light polar compounds. Here we report on the development of a new millimeter-wave resonator, covering the 150–215 GHz frequency range, and based on a low-loss corrugated waveguide with homemade highly reflective photonic mirrors obtaining a finesse above 3000 at around 164 GHz. With an effective path length of two kilometers, a significant sensitivity has been evaluated, and the detection of semi-volatile organic vapors at a trace level may be now envisaged at room temperature. We applied this technology to detect gas-phase explosive taggants and precursors, confirming a detection limit of 2 ppmv for nitromethane (NM). Leveraging the unique characteristics of the millimeter wave frequency band, we showcase highly selective detection in quasi-realistic environments of complex chemical mixtures involving explosive taggants with close chemical structures such as nitrotoluene isomers. Furthermore, we successfully address the challenge of detecting these nitro-derivative compounds vaporized from model matrices: KCl matrices, granular and plastic (NP91) explosives respectively in conventional and pyrotechnic laboratories. Our findings underscore this approach as a potent tool for practical explosive detection applications.

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引用次数: 0

A portable all-in-one microfluidic platform integrated with CRISPR-based extraction-free assay for rapid and on-site detection of monkeypox and lumpy skin disease

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-22 DOI: 10.1016/j.snb.2025.137612

Yizheng Huang , Yuhan Lu , Xiaofei Liu , Menghao Chai , Ling Yang , Kun Yin , Jiayao He , Zhijie Wang , Yajun Zhang , Yude Yu , Songyin Qiu , Yiqiang Fan , Zhao Li

Rapid and accurate identification of infectious disease pathogens plays a key role in emergency preparedness and respond to a wide range of biosecurity threats. However, current strategies require dedicated laboratories, expensive instruments and skilled operators, which are not suitable for point-of-care testing. Here, we developed an integrated CRISPR-based microfluidic platform that consisted of 3D printed bioreactors and a homemade miniaturized heater for rapid and sensitive nucleic acids detection. The all-in-one, containment-free bioreactors combined recombinase polymerase amplification, CRISPR and lateral flow dipsticks (RPA-CRISPR-LFD), allowing user-friendly operation and eliminating the need for complex laboratory equipment. The RPA-CRISPR-LFD assay was able to efficiently detect plasmids of monkeypox virus (MPXV) and lumpy skin disease virus (LSDV) down to 1 copy

μ L^{- 1}

. The platform can identify 5 extraction-free samples in parallel within 30 min, significantly reducing time consumption. In addition, we validated its clinical utility and versatility by testing 67 simulated blood or swab samples of MPXV and LSDV, yielding results consistent with real-time PCR. Thus, the platform enables rapid and convenient detection of diverse pathogens, and with the future integration of lyophilized reagents, it offers a promising tool for point-of-care testing of major biosecurity threats, especially in source-limited areas.

快速准确地识别传染病病原体在应急准备和应对各种生物安全威胁方面发挥着关键作用。然而，目前的策略需要专门的实验室、昂贵的仪器和熟练的操作人员，不适合进行床旁检测。在这里，我们开发了一种基于CRISPR技术的集成微流控平台，该平台由3D打印生物反应器和自制微型加热器组成，用于快速灵敏地检测核酸。这种一体化、无封闭性的生物反应器将重组酶聚合酶扩增、CRISPR 和侧流浸渍棒（RPA-CRISPR-LFD）结合在一起，操作方便，无需复杂的实验室设备。RPA-CRISPR-LFD 分析法能够有效地检测猴痘病毒（MPXV）和块状皮肤病病毒（LSDV）的质粒，最低可达到 1 拷贝 μL-1μL-1。该平台可在 30 分钟内同时鉴定 5 份免提取样本，大大减少了时间消耗。此外，我们还对 67 份 MPXV 和 LSDV 的模拟血液或拭子样本进行了检测，结果与实时 PCR 一致，从而验证了该平台的临床实用性和多功能性。因此，该平台能快速、方便地检测各种病原体，未来还将整合冻干试剂，为主要生物安全威胁的床旁检测提供了一种前景广阔的工具，尤其是在来源有限的地区。

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引用次数: 0

Expand detection windows for identifying single nucleotide polymorphisms using a competitive toehold-mediated strand displacement ratiometric sensing platform

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-22 DOI: 10.1016/j.snb.2025.137666

Yunshan Zhang , Sisi Bu , Fang Yang , Tuo Huang , Hao Dong , Jing Ye , Wenlin Xie , Xianzhong Feng , Diming Zhang

The subtle free energy difference introduced by a single nucleotide mutation results in poor specificity of almost all DNA hybridization probe-based single nucleotide polymorphism (SNP) detection techniques. The development of SNP biosensing strategies with both specificity and sensitivity is a hot and difficult issue in the current field. In this study, we creatively constructed a competitive toehold-mediated strand displacement sensing platform (CTMSD) based on the traditional TMSD reaction, which increased the energy barrier through the intrinsic competition mechanism and expanded the detection window of SNPs. Furthermore, based on the characteristics of the CTMSD platform, the dual-signal detection mode was introduced to change the function model of the detection curve through reporting internal reference ratio signal. The new detection curve model not only compensated for sensitivity, significantly enhanced the discrimination factor, but also greatly expanded the detection window with infinite robustness factor over the detection range. The expansion of the detection window and the improvement of specificity of CTMSD for SNP recognition based on the ratiometric signal output model were verified by computer simulations and experiments. In addition, as a deformation of the strand displacement reaction, the CTMSD was readily adaptable to commonly used signal amplification techniques, such as catalytic hairpin assembly (CHA). Through the CTMSD-CHA performance analysis and real testing of cell genomic samples, the practical application value of CTMSD with the ratiometric signal output model was confirmed. This study provides an important reference for the design and improvement of SNP biosensors and even for all nucleic acid biosensors.

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引用次数: 0

Screening and diagnosis of colorectal cancer using nucleic acid aptamers targeting Solobacterium moorei: Development of electrochemical sensors for clinical application 利用以莫雷溶杆菌为靶标的核酸适配体筛查和诊断结直肠癌：开发用于临床应用的电化学传感器

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-22 DOI: 10.1016/j.snb.2025.137669

Decai Yuan , Cheng Qiu , Hui Chen , Feng Liu , Shanshan Feng , Peiyi Zhang , Ying Qin , Tingting Fan , Yan Chen , Yuyang Jiang

Colorectal cancer (CRC) represents a significant global health issue, necessitating innovative approaches for early screening and diagnosis. Recent advances in molecular diagnostics have highlighted the potential of aptamers for use as highly specific and sensitive probes for detecting cancer biomarkers. In this study, we focus on the identification of aptamers that selectively bind to Solobacterium moorei (S. moorei), a bacterium associated with CRC. By integrating these aptamers into electrochemical sensor platforms, a reliable diagnostic tool is created for facile implementation in the clinical setting. More specifically, nucleic acid aptamers for S. moorei were obtained through whole-cell SELEX, and the affinity and specificity of these aptamers were validated. Subsequently, two types of electrochemical sensors were developed. Firstly, an electrochemical impedance spectroscopy sensor was developed to detect impedance changes on the electrode surface, which were caused by the binding of S. moorei to the aptamer. Secondly, a CRISPR/Cas12a-based electrochemical aptasensor was developed based on the ability of the Cas12a enzyme to be activated by specific single-stranded DNA, triggering its trans-cleavage activity. The limits of detection of these sensors for S. moorei were 30 and 6 CFU/mL, respectively. Clinical validation was performed using patient samples to assess the sensor efficacy in a real-world setting. The obtained results suggested that the abundance of S. moorei in the feces of CRC patients was significantly greater compared to that of healthy individuals. This integration of S. moorei aptamers into electrochemical sensors offers a non-invasive and cost-effective alternative to current screening methods available for CRC.

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引用次数: 0

A membrane-protein targeted DNA multitasking processor for precise tumor cell imaging 用于肿瘤细胞精确成像的膜蛋白靶向 DNA 多任务处理器

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-22 DOI: 10.1016/j.snb.2025.137667

Zhihao Li, Xue Wang, Xiaojuan Wang, Yang Shu

DNA nanodevices based on nucleic acid aptamers have become powerful tools for tumor imaging. However, the problem of “on-target, off-tumor” has always been a challenge for intelligent molecular probes to identify tumors. In order to achieve precise identification and tumor microenvironment (TEM) localization of cancer cells, we integrated membrane protein aptamers with two reporter molecules, imotif (pH reporter) and ATP aptamer (ATP reporter), which can respond to specific biomarkers in TME, and developed a Y-shaped multitasking processor (Y-smp). This Y-shaped multitasking processor can target membrane protein overexpressed on the surface of cancer cells, allowing them to remain on the cell surface. At the same time, the two reporter probes work independently in response to acidity and ATP, undergo conformational changes and detach from Y-smp, carrying away quenching groups to produce green fluorescence signals corresponding to ATP molecules and red fluorescence signals corresponding to acidity. The Y-shaped multitasking processor can generate different fluorescent signals corresponding to two microenvironment markers, and does not interfere with each other in a simulated tumor microenvironment characterized by weak acidity and abundant ATP. The simple and intuitive molecular probe recognition strategy provides the possibility for precise localization and recognition of cancer cells.

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引用次数: 0

Illuminating potassium dynamics in endocytic pathways using FRET biosensors and computational tools

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-22 DOI: 10.1016/j.snb.2025.137668

Xiaoqi Hu , Kiseok Han , Gyuho Choi , Myungeun Suk , Tae-Jin Kim

Fluorescence resonance energy transfer (FRET)-based live-cell imaging and genetically encoded biosensors are widely used to study biochemical processes in living cells. However, there remains a gap in robust analytical methods for examining complex processes like endosomal trafficking. In this study, we introduce two advanced tools—EasyFRET and EndoAna—specifically designed for FRET image analysis, along with two novel potassium biosensors, GalT-KIRIN and KIRIN-Lamp1, targeted to endosomes and lysosomes, respectively. Together, these tools establish a comprehensive workflow for investigating potassium signaling, which plays a critical role in cellular homeostasis and various biological functions. Our live-cell imaging results demonstrate that EasyFRET and EndoAna provide efficient and reliable FRET image analysis, while the potassium indicators offer valuable insights into potassium dynamics within the endosomal trafficking system.

引用次数: 0

In-situ detection of dissolved C2H2/CH4 with frequency-division-multiplexed fiber-optic photoacoustic sensor

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-21 DOI: 10.1016/j.snb.2025.137651

Chenxi Li , Fengxiang Ma , Chun Sun , Hongchao Qi , Xiao Han , Min Guo , Ke Chen

An in-situ detection system of dissolved C₂H₂/CH₄ with a frequency division-multiplexed fiber-optic photoacoustic (PA) sensor (FOPAS) is designed for diagnosing failures of large power transformers. The system relies on a fluorinated ethylene-propylene (FEP) membrane to extract gases and an all-optical gas sensing element, which has the advantages of non-consumption of oil, anti-electromagnetic interference and passive operation. The oil-gas separation unit and the PA excitation-detection unit are closely integrated into an independent system, communicating with a dual-frequency demodulator through two optical fibers. Two lasers emitting at 1532.83 nm and 1650.91 nm operate simultaneously, exciting PA signals of C₂H₂ and CH₄, respectively. The modulation frequencies of the two lasers are 1486 Hz and 1490 Hz, and twice the frequencies fall within the resonant frequency band of the fiber-optic microphone. A custom-designed dual-channel digital lock-in amplifier is embedded in the demodulator to avoid crosstalk between frequencies, which achieves dual-component synchronous detection. The experimental results show that the temperature increase can promote oil-gas separation. The system can reach equilibrium within 2 h at 60 ℃. The minimum detectable concentrations of dissolved C₂H₂ and CH₄ are both about 0.1 μL·L⁻¹, which meet the detection requirements of dissolved gases in transformer oil. The system has the potential for real-time monitoring of dissolved C₂H₂/CH₄. The excellent detection performance provides technical support for the more accurate real-time condition monitoring and early fault warning of large power transformers.

利用频分复用光纤光声（PA）传感器（FOPAS）设计了一种溶解的 C2H2/CH4 原位检测系统，用于诊断大型电力变压器的故障。该系统依靠氟化乙烯-丙烯（FEP）膜和全光气体传感元件提取气体，具有不耗油、抗电磁干扰和无源操作等优点。油气分离单元和 PA 激发-检测单元紧密结合成一个独立的系统，通过两根光纤与双频解调器通信。两个波长分别为 1532.83 纳米和 1650.91 纳米的激光器同时工作，分别激发 C2H2 和 CH4 的 PA 信号。两束激光的调制频率分别为 1486 Hz 和 1490 Hz，其中两束频率位于光纤传声器的谐振频带内。解调器中嵌入了一个定制设计的双通道数字锁相放大器，以避免频率之间的串扰，从而实现双分量同步检测。实验结果表明，温度升高可促进油气分离。在 60 ℃ 下，系统可在 2 小时内达到平衡。溶解的 C2H2 和 CH4 的最低检测浓度均约为 0.1 μL-L-1，满足变压器油中溶解气体的检测要求。该系统具有实时监测溶解的 C2H2/CH4 的潜力。优异的检测性能为大型电力变压器更精确的实时状态监测和早期故障预警提供了技术支持。

{"title":"In-situ detection of dissolved C2H2/CH4 with frequency-division-multiplexed fiber-optic photoacoustic sensor","authors":"Chenxi Li , Fengxiang Ma , Chun Sun , Hongchao Qi , Xiao Han , Min Guo , Ke Chen","doi":"10.1016/j.snb.2025.137651","DOIUrl":"10.1016/j.snb.2025.137651","url":null,"abstract":"<div><div>An <em>in-situ</em> detection system of dissolved C<sub>2</sub>H<sub>2</sub>/CH<sub>4</sub> with a frequency division-multiplexed fiber-optic photoacoustic (PA) sensor (FOPAS) is designed for diagnosing failures of large power transformers. The system relies on a fluorinated ethylene-propylene (FEP) membrane to extract gases and an all-optical gas sensing element, which has the advantages of non-consumption of oil, anti-electromagnetic interference and passive operation. The oil-gas separation unit and the PA excitation-detection unit are closely integrated into an independent system, communicating with a dual-frequency demodulator through two optical fibers. Two lasers emitting at 1532.83 nm and 1650.91 nm operate simultaneously, exciting PA signals of C<sub>2</sub>H<sub>2</sub> and CH<sub>4</sub>, respectively. The modulation frequencies of the two lasers are 1486 Hz and 1490 Hz, and twice the frequencies fall within the resonant frequency band of the fiber-optic microphone. A custom-designed dual-channel digital lock-in amplifier is embedded in the demodulator to avoid crosstalk between frequencies, which achieves dual-component synchronous detection. The experimental results show that the temperature increase can promote oil-gas separation. The system can reach equilibrium within 2 h at 60 ℃. The minimum detectable concentrations of dissolved C<sub>2</sub>H<sub>2</sub> and CH<sub>4</sub> are both about 0.1 μL·L<sup>−1</sup>, which meet the detection requirements of dissolved gases in transformer oil. The system has the potential for real-time monitoring of dissolved C<sub>2</sub>H<sub>2</sub>/CH<sub>4</sub>. The excellent detection performance provides technical support for the more accurate real-time condition monitoring and early fault warning of large power transformers.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137651"},"PeriodicalIF":8.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Au atomic clusters engineered on sea urchin-like In6WO12 nanospheres for high-performance ppb-level NO2 sensing at room temperature

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-21 DOI: 10.1016/j.snb.2025.137653

Fei Liu , Jiurong Liu , Jinbo Zhao , Zhidong Jin , Shiqiang Li , Lin Liu , Zhou Wang , Lili Wu

Conventional metal oxide semiconductor sensors frequently face challenges in detecting trace harmful gases at room temperature (RT) due to their limited sensing capabilities. Addressing this challenge, we construct a novel binary metal oxide sensors based on In₆WO₁₂ for the detection of NO₂ at RT according to the synergistic effect of effective volume depletion and electron scattering. The sea urchin-like In₆WO₁₂ nanospheres with large surface area (124.5 cm²g⁻¹) and multiple diffusion paths are assembled from 7.5 nm nanoparticles via an ethylenediamine-assisted coprecipitation method. Additionally, the decorating of Au atomic cluster with ∼4.5 nm further optimizes the surface reaction path of NO₂. The 1 wt%Au-In₆WO₁₂ sensor demonstrates a remarkably high response value (203) to 2 ppm NO₂ at RT, which was 54.8 times greater than that of the pristine In₆WO₁₂. Significantly, the sensor also shows exceptional selectivity, with a selectivity coefficient exceeding 98 %, and it can detect NO₂ at concentrations as low as 1.73 ppb, outperforming state-of-the-art conventional MOS-based NO₂ sensors. In-situ DRIFTS and energy band structures analyses confirm surface reaction processes of NO₂ and elucidate the reasons for the optimization of surface reactions. Reaction kinetics calculations indicate that the reaction process is accelerated due to the anchoring of Au atomic clusters. The unprecedented NO₂ sensing performances of the 1 wt%Au-In₆WO₁₂ sensor renders it an exceptional choice for precise real-time detection of ppb-level NO₂ at RT, and offers a novel strategy to enhance NO₂ sensing properties.

{"title":"Au atomic clusters engineered on sea urchin-like In6WO12 nanospheres for high-performance ppb-level NO2 sensing at room temperature","authors":"Fei Liu , Jiurong Liu , Jinbo Zhao , Zhidong Jin , Shiqiang Li , Lin Liu , Zhou Wang , Lili Wu","doi":"10.1016/j.snb.2025.137653","DOIUrl":"10.1016/j.snb.2025.137653","url":null,"abstract":"<div><div>Conventional metal oxide semiconductor sensors frequently face challenges in detecting trace harmful gases at room temperature (RT) due to their limited sensing capabilities. Addressing this challenge, we construct a novel binary metal oxide sensors based on In<sub>6</sub>WO<sub>12</sub> for the detection of NO<sub>2</sub> at RT according to the synergistic effect of effective volume depletion and electron scattering. The sea urchin-like In<sub>6</sub>WO<sub>12</sub> nanospheres with large surface area (124.5 cm<sup>2</sup>g<sup>−1</sup>) and multiple diffusion paths are assembled from 7.5 nm nanoparticles via an ethylenediamine-assisted coprecipitation method. Additionally, the decorating of Au atomic cluster with ∼4.5 nm further optimizes the surface reaction path of NO<sub>2</sub>. The 1 wt%Au-In<sub>6</sub>WO<sub>12</sub> sensor demonstrates a remarkably high response value (203) to 2 ppm NO<sub>2</sub> at RT, which was 54.8 times greater than that of the pristine In<sub>6</sub>WO<sub>12</sub>. Significantly, the sensor also shows exceptional selectivity, with a selectivity coefficient exceeding 98 %, and it can detect NO<sub>2</sub> at concentrations as low as 1.73 ppb, outperforming state-of-the-art conventional MOS-based NO<sub>2</sub> sensors. In-situ DRIFTS and energy band structures analyses confirm surface reaction processes of NO<sub>2</sub> and elucidate the reasons for the optimization of surface reactions. Reaction kinetics calculations indicate that the reaction process is accelerated due to the anchoring of Au atomic clusters. The unprecedented NO<sub>2</sub> sensing performances of the 1 wt%Au-In<sub>6</sub>WO<sub>12</sub> sensor renders it an exceptional choice for precise real-time detection of ppb-level NO<sub>2</sub> at RT, and offers a novel strategy to enhance NO<sub>2</sub> sensing properties.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137653"},"PeriodicalIF":8.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on Response Characteristic of Ionized Particle Sensor Array in Flue Gas Emission 电离粒子传感器阵列在烟气排放中的响应特性研究

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-21 DOI: 10.1016/j.snb.2025.137608

Muhammad Waqas , Yong Zhang , Xiaoyu Li , Saif Aldeen Saad Obayes Al-Kadhim

Power plant emissions, including PM₁, PM_2.5, PM₄, PM₁₀, SO₂, and NO, pose significant health risks. Precise sensing is crucial for identifying these sources; however, conventional detection methods are often complex and bulky, making them inadequate for detecting particulate matter in flue gases. Here, we purposed an array of eight silicon micron-column, three-electrode ionization particle sensors designed for simultaneous measurement of particulate concentrations, gas components, and temperature. In response to exhaust contaminants, high-frequency impulse voltage induces gas discharge between the electrodes, resulting in substantial positive ion production via field-induced and diffusion charging modes. The concentration gradient drives positively charged particles toward the extracting electrode, with opposing forces E₁ and E₂ accelerating ions from the ionization to the collecting region, generating collecting currents that directly measure the concentrations of both particles, and gases simultaneously. The array utilizes the nonlinear relationship between discharge current and electrode separation to measure multiple parameters, enabling the selective detection of eight contaminants. A support vector machine algorithm analyzes particle concentration values, demonstrating high sensitivity to PM_2.5 and PM₁₀, with a reference error below 10%. This cost-effective sensor array directly measures exhaust contaminants, demonstrating its potential for addressing environmental challenges.

{"title":"Study on Response Characteristic of Ionized Particle Sensor Array in Flue Gas Emission","authors":"Muhammad Waqas , Yong Zhang , Xiaoyu Li , Saif Aldeen Saad Obayes Al-Kadhim","doi":"10.1016/j.snb.2025.137608","DOIUrl":"10.1016/j.snb.2025.137608","url":null,"abstract":"<div><div>Power plant emissions, including PM<sub>1</sub>, PM<sub>2.5</sub>, PM<sub>4</sub>, PM<sub>10</sub>, SO<sub>2</sub>, and NO, pose significant health risks. Precise sensing is crucial for identifying these sources; however, conventional detection methods are often complex and bulky, making them inadequate for detecting particulate matter in flue gases. Here, we purposed an array of eight silicon micron-column, three-electrode ionization particle sensors designed for simultaneous measurement of particulate concentrations, gas components, and temperature. In response to exhaust contaminants, high-frequency impulse voltage induces gas discharge between the electrodes, resulting in substantial positive ion production via field-induced and diffusion charging modes. The concentration gradient drives positively charged particles toward the extracting electrode, with opposing forces <em>E</em><sub>1</sub> and <em>E</em><sub>2</sub> accelerating ions from the ionization to the collecting region, generating collecting currents that directly measure the concentrations of both particles, and gases simultaneously. The array utilizes the nonlinear relationship between discharge current and electrode separation to measure multiple parameters, enabling the selective detection of eight contaminants. A support vector machine algorithm analyzes particle concentration values, demonstrating high sensitivity to PM<sub>2.5</sub> and PM<sub>10</sub>, with a reference error below 10%. This cost-effective sensor array directly measures exhaust contaminants, demonstrating its potential for addressing environmental challenges.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"436 ","pages":"Article 137608"},"PeriodicalIF":8.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143672634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated Papanicolaou staining system (PapDisc) based on centrifugal microfluidics using cut-off valves

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-03-21 DOI: 10.1016/j.snb.2025.137626

Ahamdreza Jahanian , Esmail Pishbin , Shahriar Dabiri , Amid Rahi , Mahdi Navidbakhsh

Cervical cancer, primarily caused by the human papillomavirus (HPV), remains a significant global health issue, especially in underserved regions. Despite the availability of screening methods, barriers such as limited access and resource constraints contribute to late-stage diagnoses and high mortality rates. This study introduces a novel platform utilizing centrifugal microfluidics to automate the labor-intensive Pap smear staining process, providing a cost-effective and accessible solution. The system features a disc-shaped cartridge preloaded with staining dyes and fixation liquids, following the Papanicolaou (Pap) smear staining protocol. The sample preparation is executed by applying a specific rotational speed profile via a rotor. A novel valving mechanism (Cut-off valve), driven by centrifugal and magnetic forces, acts as the primary fluidic unit to control the precise and sequential staining protocol. The functionality of these valves is demonstrated through numerical simulations for obtaining the rotational speed, using the intensity of the magnetic flux density

\overset{̅}{B}

around several different dimension magnets along with experimental validations. This platform presents a practical and scalable approach to cervical cancer screening, with the potential to enhance diagnostic accuracy and accessibility, particularly in low-resource settings.

{"title":"Automated Papanicolaou staining system (PapDisc) based on centrifugal microfluidics using cut-off valves","authors":"Ahamdreza Jahanian , Esmail Pishbin , Shahriar Dabiri , Amid Rahi , Mahdi Navidbakhsh","doi":"10.1016/j.snb.2025.137626","DOIUrl":"10.1016/j.snb.2025.137626","url":null,"abstract":"<div><div>Cervical cancer, primarily caused by the human papillomavirus (HPV), remains a significant global health issue, especially in underserved regions. Despite the availability of screening methods, barriers such as limited access and resource constraints contribute to late-stage diagnoses and high mortality rates. This study introduces a novel platform utilizing centrifugal microfluidics to automate the labor-intensive Pap smear staining process, providing a cost-effective and accessible solution. The system features a disc-shaped cartridge preloaded with staining dyes and fixation liquids, following the Papanicolaou (Pap) smear staining protocol. The sample preparation is executed by applying a specific rotational speed profile via a rotor. A novel valving mechanism (Cut-off valve), driven by centrifugal and magnetic forces, acts as the primary fluidic unit to control the precise and sequential staining protocol. The functionality of these valves is demonstrated through numerical simulations for obtaining the rotational speed, using the intensity of the magnetic flux density <span><math><mover><mrow><mi>B</mi></mrow><mo>̅</mo></mover></math></span> around several different dimension magnets along with experimental validations. This platform presents a practical and scalable approach to cervical cancer screening, with the potential to enhance diagnostic accuracy and accessibility, particularly in low-resource settings.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"435 ","pages":"Article 137626"},"PeriodicalIF":8.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0