Sensors and Actuators B: Chemical最新文献_第8页

Global DNA methylation level sensing using methyl-CpG binding domain-fused luciferase and fluorescent protein

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-12 DOI: 10.1016/j.snb.2025.137797

Yuto Shoji , Yuno Ikeda , Rie Rin , Koichiro Doi , Masayoshi Tanaka , Wataru Yoshida

Methylation at the 5-position of cytosine in CpG dinucleotides is involved in the regulation of gene expression. In cancer cells, aberrant CpG methylation patterns are observed, characterized by promoter hypermethylation on tumor suppressor genes and global hypomethylation inducing genomic instability. We previously reported that global DNA methylation levels were quantified by bioluminescence resonance energy transfer (BRET) between methyl-CpG binding domain (MBD)-fused firefly luciferase and DNA intercalating dye. The assay simply and accurately detects global DNA methylation levels, but requires a 30-min incubation period for the DNA intercalating dye to bind to genomic DNA. In this study, a rapid system for measuring global DNA methylation levels was developed using MBD-fused NanoLuc luciferase (Nluc) and MBD-fused fluorescent protein, monomeric Venus (mVenus). When the MBD-fused Nluc and MBD-fused mVenus simultaneously bind to adjacent methyl-CpGs, the amount of which is associated with the global DNA methylation level, BRET occurs between two. We demonstrated that the BRET signal between MBD-fused Nluc and MBD-fused mVenus was detected on double-stranded DNA containing two methyl-CpG sites, and the efficiency depended on the distance between two methyl-CpG sites. In addition, global DNA methylation levels were quantified within 3 min by the BRET assay (R² = 0.99). These results indicate that the global DNA methylation levels can be easily, accurately, and rapidly quantified by the BRET assay using MBD-fused Nluc and MBD-fused mVenus.

{"title":"Global DNA methylation level sensing using methyl-CpG binding domain-fused luciferase and fluorescent protein","authors":"Yuto Shoji , Yuno Ikeda , Rie Rin , Koichiro Doi , Masayoshi Tanaka , Wataru Yoshida","doi":"10.1016/j.snb.2025.137797","DOIUrl":"10.1016/j.snb.2025.137797","url":null,"abstract":"<div><div>Methylation at the 5-position of cytosine in CpG dinucleotides is involved in the regulation of gene expression. In cancer cells, aberrant CpG methylation patterns are observed, characterized by promoter hypermethylation on tumor suppressor genes and global hypomethylation inducing genomic instability. We previously reported that global DNA methylation levels were quantified by bioluminescence resonance energy transfer (BRET) between methyl-CpG binding domain (MBD)-fused firefly luciferase and DNA intercalating dye. The assay simply and accurately detects global DNA methylation levels, but requires a 30-min incubation period for the DNA intercalating dye to bind to genomic DNA. In this study, a rapid system for measuring global DNA methylation levels was developed using MBD-fused NanoLuc luciferase (Nluc) and MBD-fused fluorescent protein, monomeric Venus (mVenus). When the MBD-fused Nluc and MBD-fused mVenus simultaneously bind to adjacent methyl-CpGs, the amount of which is associated with the global DNA methylation level, BRET occurs between two. We demonstrated that the BRET signal between MBD-fused Nluc and MBD-fused mVenus was detected on double-stranded DNA containing two methyl-CpG sites, and the efficiency depended on the distance between two methyl-CpG sites. In addition, global DNA methylation levels were quantified within 3min by the BRET assay (<em>R</em><sup>2</sup> = 0.99). These results indicate that the global DNA methylation levels can be easily, accurately, and rapidly quantified by the BRET assay using MBD-fused Nluc and MBD-fused mVenus.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"438 ","pages":"Article 137797"},"PeriodicalIF":8.0,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Kresling origami-enabled soft robot toward autonomous obstacle avoidance and wall-climbing

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-12 DOI: 10.1016/j.snb.2025.137792

Sicheng Chen , Zhilin Yu , Alin Duan , Amèvi Tongne , Yahui Li , Wenling Zhang

In recent years, soft robotics has rapidly emerged as a prominent research topic, unlocking new possibilities for addressing real-world challenges. However, enabling soft robots to achieve autonomous motion in confined spaces remains a significant hurdle. To address this, we propose a highly integrated soft robot capable of wall-climbing transitional motion and tactile perception in narrow spaces, equipped with closed-loop sensing and control capabilities. The robot's mechanical structure leverages the ingenious design of the Kresling origami architecture combined with flexible materials, enabling smooth transitional movements from flat-surface crawling to various angles ranging from 0° to 90° within a confined 2 cm space. Furthermore, by in-situ integrating a highly sensitive and flexible planar capacitive contact sensor at the robot's tip, it can rapidly detect and respond to obstacles. This customized sensor exhibits an impressive response time and maintains durability over 1000 cycles. The real-time sensing data is fed back to the controller, facilitating closed-loop obstacle avoidance and autonomous motion. This innovative design, combining advanced mechanical architecture with a closed-loop sensing strategy, provides a promising pathway for developing soft robots capable of autonomous operation in constrained and dynamic environments.

{"title":"A Kresling origami-enabled soft robot toward autonomous obstacle avoidance and wall-climbing","authors":"Sicheng Chen , Zhilin Yu , Alin Duan , Amèvi Tongne , Yahui Li , Wenling Zhang","doi":"10.1016/j.snb.2025.137792","DOIUrl":"10.1016/j.snb.2025.137792","url":null,"abstract":"<div><div>In recent years, soft robotics has rapidly emerged as a prominent research topic, unlocking new possibilities for addressing real-world challenges. However, enabling soft robots to achieve autonomous motion in confined spaces remains a significant hurdle. To address this, we propose a highly integrated soft robot capable of wall-climbing transitional motion and tactile perception in narrow spaces, equipped with closed-loop sensing and control capabilities. The robot's mechanical structure leverages the ingenious design of the Kresling origami architecture combined with flexible materials, enabling smooth transitional movements from flat-surface crawling to various angles ranging from 0° to 90° within a confined 2 cm space. Furthermore, by in-situ integrating a highly sensitive and flexible planar capacitive contact sensor at the robot's tip, it can rapidly detect and respond to obstacles. This customized sensor exhibits an impressive response time and maintains durability over 1000 cycles. The real-time sensing data is fed back to the controller, facilitating closed-loop obstacle avoidance and autonomous motion. This innovative design, combining advanced mechanical architecture with a closed-loop sensing strategy, provides a promising pathway for developing soft robots capable of autonomous operation in constrained and dynamic environments.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"438 ","pages":"Article 137792"},"PeriodicalIF":8.0,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143822924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Piezoelectric micro diaphragm based high performance humidity sensor

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-11 DOI: 10.1016/j.snb.2025.137760

Jihang Liu , Doris Keh Ting Ng , Yul Koh , Subhranu Samanta , Linfang Xu , Md Hazwani Khairy Md Husni , Merugu Srinivas , Alberto Leotti , Young Jik Hur , Qingxin Zhang , Yao Zhu

This paper presents a novel humidity sensor based on a piezoelectric micro diaphragm (PMD) resonator integrated with a zinc oxide (ZnO) sensing layer, demonstrating exceptional performance through stress-based sensing mechanisms. Unlike conventional mass-loading based sensors, our design uniquely leverages the PMD's flexible vibration modes combined with a ZnO sensing layer to achieve unprecedented sensitivity. The sensor exhibits dual-mode operation at low frequencies of 0.17 MHz and 0.68 MHz, where the second mode demonstrates remarkable humidity sensitivity reaching 638–4110 Hz/%RH and achieves outstanding figure of merit (FOM) values of 943–6080 ppm/%RH from low relative humidity range (0.6 % RH∼80 % RH) to high relative humidity range (80 % RH∼90 % RH), surpassing current state-of-the-art technologies by more than an order of magnitude. Systematic characterization reveals fast response characteristics with T₆₃ absorption/desorption times of 5/11 s and excellent selectivity against common gases. The enhanced performance of the second mode is attributed to its complex modal pattern enabling more effective stress distribution, as verified through finite element analysis and experimental validation. This work establishes PMD architecture as a promising platform for high-performance humidity sensing applications, offering an optimal balance between sensitivity, response time, and system integration capabilities. Additionally, the versatile stress-based sensing mechanism can be adapted for detecting various gas molecules of societal importance by simply modifying the sensing layer, enabling broader environmental monitoring applications.

{"title":"Piezoelectric micro diaphragm based high performance humidity sensor","authors":"Jihang Liu , Doris Keh Ting Ng , Yul Koh , Subhranu Samanta , Linfang Xu , Md Hazwani Khairy Md Husni , Merugu Srinivas , Alberto Leotti , Young Jik Hur , Qingxin Zhang , Yao Zhu","doi":"10.1016/j.snb.2025.137760","DOIUrl":"10.1016/j.snb.2025.137760","url":null,"abstract":"<div><div>This paper presents a novel humidity sensor based on a piezoelectric micro diaphragm (PMD) resonator integrated with a zinc oxide (ZnO) sensing layer, demonstrating exceptional performance through stress-based sensing mechanisms. Unlike conventional mass-loading based sensors, our design uniquely leverages the PMD's flexible vibration modes combined with a ZnO sensing layer to achieve unprecedented sensitivity. The sensor exhibits dual-mode operation at low frequencies of 0.17 MHz and 0.68 MHz, where the second mode demonstrates remarkable humidity sensitivity reaching 638–4110 Hz/%RH and achieves outstanding figure of merit (FOM) values of 943–6080 ppm/%RH from low relative humidity range (0.6 % RH∼80 % RH) to high relative humidity range (80 % RH∼90 % RH), surpassing current state-of-the-art technologies by more than an order of magnitude. Systematic characterization reveals fast response characteristics with T<sub>63</sub> absorption/desorption times of 5/11 s and excellent selectivity against common gases. The enhanced performance of the second mode is attributed to its complex modal pattern enabling more effective stress distribution, as verified through finite element analysis and experimental validation. This work establishes PMD architecture as a promising platform for high-performance humidity sensing applications, offering an optimal balance between sensitivity, response time, and system integration capabilities. Additionally, the versatile stress-based sensing mechanism can be adapted for detecting various gas molecules of societal importance by simply modifying the sensing layer, enabling broader environmental monitoring applications.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"438 ","pages":"Article 137760"},"PeriodicalIF":8.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Balancing the p- and n-type conductivities of the ZnO/graft-poly(3-hexylthiophene) copolymer nanocomposite to achieve sub-ppb NO2 detection in chemiresistive sensors at room temperature

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-11 DOI: 10.1016/j.snb.2025.137726

Piotr Kałużyński , Marcin Procek , Agnieszka Stolarczyk , Karolina Głosz , Tomasz Jarosz

Hybrid organic/inorganic composites are a frequent material used in chemoresistive sensors. These composites typically contain components that exhibit p- and n-type electrical conductivity, so as to enable the formation of p–n junctions and increase the sensitivity of the sensors. The specific mechanism behind the operation of such junctions appears to be well-understood and is rarely examined on a case-by-case basis. The interactions between p- and n-type conductors, however, need not be beneficial to the performance of the sensor. In this work, we provide evidence of the competition between p- and n-type conductivities of a hybrid nanocomposite activated by UV light, subjected to ageing and provide a mechanistic description of the underlying processes, as well as indicate potential for such phenomena to be harnessed in development of future sensor generations. By using nanocomposite consist of nanostructured ZnO and poly(3-hexylthiophene) based graft-comb-copolymers we obtained sensor material that can achieve sub-ppb detection limit of NO₂ (as low as 50 ppt) capabilities at room temperature. It is experimentally demonstrated that proper choice of polymer material used in the nanocomposite provide stabilisation of baseline drift and lowers limit of the detection while ensuring good adhesion of the receptor layer to the substrate.

{"title":"Balancing the p- and n-type conductivities of the ZnO/graft-poly(3-hexylthiophene) copolymer nanocomposite to achieve sub-ppb NO2 detection in chemiresistive sensors at room temperature","authors":"Piotr Kałużyński , Marcin Procek , Agnieszka Stolarczyk , Karolina Głosz , Tomasz Jarosz","doi":"10.1016/j.snb.2025.137726","DOIUrl":"10.1016/j.snb.2025.137726","url":null,"abstract":"<div><div>Hybrid organic/inorganic composites are a frequent material used in chemoresistive sensors. These composites typically contain components that exhibit p- and n-type electrical conductivity, so as to enable the formation of p–n junctions and increase the sensitivity of the sensors. The specific mechanism behind the operation of such junctions appears to be well-understood and is rarely examined on a case-by-case basis. The interactions between p- and n-type conductors, however, need not be beneficial to the performance of the sensor. In this work, we provide evidence of the competition between p- and n-type conductivities of a hybrid nanocomposite activated by UV light, subjected to ageing and provide a mechanistic description of the underlying processes, as well as indicate potential for such phenomena to be harnessed in development of future sensor generations. By using nanocomposite consist of nanostructured ZnO and poly(3-hexylthiophene) based graft-comb-copolymers we obtained sensor material that can achieve sub-ppb detection limit of NO<sub>2</sub> (as low as 50 ppt) capabilities at room temperature. It is experimentally demonstrated that proper choice of polymer material used in the nanocomposite provide stabilisation of baseline drift and lowers limit of the detection while ensuring good adhesion of the receptor layer to the substrate.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"438 ","pages":"Article 137726"},"PeriodicalIF":8.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143822944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrowetting on swollen dielectric-hydrophobic composite PDMS film for digital microfluidics

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-11 DOI: 10.1016/j.snb.2025.137793

Xiaodong He, Qingyuan Huang, Jingsong Xu

Polydimethylsiloxane (PDMS), a commonly used material, serves as both a dielectric layer and a hydrophobic surface in digital microfluidics (DMF) systems. However, its low dielectric constant results in suboptimal electrowetting-on-dielectric (EWOD) performance, limiting its application in DMF systems. In this study, a novel dielectric-hydrophobic integrated film, termed swollen composite PDMS (S-CPDMS), was developed. This film was fabricated by combining the ferroelectric polymer Poly(vinylidene fluoride-co-trifluoroethylene) (P(VDF-TrFE)) with PDMS and enhancing surface hydrophobicity and smoothness through the application of silicone oil as a liquid lubricant. Experimental results demonstrated that the S-CPDMS film exhibits excellent hydrophobicity, high optical transparency, anti-biofouling properties, and smooth sliding performance. The effects of film composition, applied voltage frequency, and vertical placement time after silicone oil injection on its EWOD behavior were systematically investigated. The findings revealed that the S-CPDMS film achieves optimal EWOD performance when the P(VDF-TrFE) to PDMS ratio is 3:1. Furthermore, under a 1 kHz square wave alternating current (AC), the droplets on the film exhibited strong EWOD responsiveness and high reversibility. Using the S-CPDMS film, a DMF device was constructed, successfully enabling droplet manipulation in both open and closed environments. Finally, glucose colorimetric reactions were successfully performed in the DMF device utilizing the S-CPDMS film. This dielectric-hydrophobic integrated film offers a simple and cost-effective solution for integrating dielectric and hydrophobic layers in DMF systems, opening new possibilities for developing lab-on-a-chip platforms.

{"title":"Electrowetting on swollen dielectric-hydrophobic composite PDMS film for digital microfluidics","authors":"Xiaodong He, Qingyuan Huang, Jingsong Xu","doi":"10.1016/j.snb.2025.137793","DOIUrl":"10.1016/j.snb.2025.137793","url":null,"abstract":"<div><div>Polydimethylsiloxane (PDMS), a commonly used material, serves as both a dielectric layer and a hydrophobic surface in digital microfluidics (DMF) systems. However, its low dielectric constant results in suboptimal electrowetting-on-dielectric (EWOD) performance, limiting its application in DMF systems. In this study, a novel dielectric-hydrophobic integrated film, termed swollen composite PDMS (S-CPDMS), was developed. This film was fabricated by combining the ferroelectric polymer Poly(vinylidene fluoride-co-trifluoroethylene) (P(VDF-TrFE)) with PDMS and enhancing surface hydrophobicity and smoothness through the application of silicone oil as a liquid lubricant. Experimental results demonstrated that the S-CPDMS film exhibits excellent hydrophobicity, high optical transparency, anti-biofouling properties, and smooth sliding performance. The effects of film composition, applied voltage frequency, and vertical placement time after silicone oil injection on its EWOD behavior were systematically investigated. The findings revealed that the S-CPDMS film achieves optimal EWOD performance when the P(VDF-TrFE) to PDMS ratio is 3:1. Furthermore, under a 1 kHz square wave alternating current (AC), the droplets on the film exhibited strong EWOD responsiveness and high reversibility. Using the S-CPDMS film, a DMF device was constructed, successfully enabling droplet manipulation in both open and closed environments. Finally, glucose colorimetric reactions were successfully performed in the DMF device utilizing the S-CPDMS film. This dielectric-hydrophobic integrated film offers a simple and cost-effective solution for integrating dielectric and hydrophobic layers in DMF systems, opening new possibilities for developing lab-on-a-chip platforms.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"438 ","pages":"Article 137793"},"PeriodicalIF":8.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemiresistive triethylamine sensor based on Rh2O3-loaded core-shell LaFeO3 porous spheres and DFT study to explain its behavior

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-11 DOI: 10.1016/j.snb.2025.137791

Junkai Shao , Shangyan Wang , Yuhang Qi , Guofeng Pan , Xueli Yang , Lianjun Hu , Meiyan Qiu

In this work, LaFeO₃ nanopowders with a core-shell structure were successfully synthesized using a facile hydrothermal method combined with wet impregnation to load varying amounts of Rh₂O₃ nanoparticles on the surface of core-shell spheres. Characterization results confirm that the synthesized Rh₂O₃-Fe₂O₃/LaFeO₃ samples have a core-shell structure with uniformly distributed Rh₂O₃ nanoparticles on the surface. In gas sensing studies, the sensor based on 2 at% Rh₂O₃-Fe₂O₃/LaFeO₃ core-shell spheres exhibited significantly enhanced performance (more than four times higher) for detecting triethylamine (TEA) compared to pristine Fe₂O₃/LaFeO₃ core-shell microspheres. At an operating temperature of 200°C, the sensor showed a response value of 237 for 100 ppm TEA with a very low detection limit (50 ppb, 1.2) and much faster response/recovery time (47 s/12 s). The performance improvement is due to changes in the potential barrier at the Rh₂O₃ and Fe₂O₃/LaFeO₃ interface, enhancing charge transfer. Additionally, based on first principles calculations, this work examines the adsorption energy and possible adsorption configurations of adsorbates. The Rh element acts as a negative charge center, which facilitates gas adsorption and reactions on the surface via a spillover effect. This study offers a promising candidate for TEA sensing and theoretical insights into high-performance sensor development.

{"title":"Chemiresistive triethylamine sensor based on Rh2O3-loaded core-shell LaFeO3 porous spheres and DFT study to explain its behavior","authors":"Junkai Shao , Shangyan Wang , Yuhang Qi , Guofeng Pan , Xueli Yang , Lianjun Hu , Meiyan Qiu","doi":"10.1016/j.snb.2025.137791","DOIUrl":"10.1016/j.snb.2025.137791","url":null,"abstract":"<div><div>In this work, LaFeO<sub>3</sub> nanopowders with a core-shell structure were successfully synthesized using a facile hydrothermal method combined with wet impregnation to load varying amounts of Rh<sub>2</sub>O<sub>3</sub> nanoparticles on the surface of core-shell spheres. Characterization results confirm that the synthesized Rh<sub>2</sub>O<sub>3</sub>-Fe<sub>2</sub>O<sub>3</sub>/LaFeO<sub>3</sub> samples have a core-shell structure with uniformly distributed Rh<sub>2</sub>O<sub>3</sub> nanoparticles on the surface. In gas sensing studies, the sensor based on 2 at% Rh<sub>2</sub>O<sub>3</sub>-Fe<sub>2</sub>O<sub>3</sub>/LaFeO<sub>3</sub> core-shell spheres exhibited significantly enhanced performance (more than four times higher) for detecting triethylamine (TEA) compared to pristine Fe<sub>2</sub>O<sub>3</sub>/LaFeO<sub>3</sub> core-shell microspheres. At an operating temperature of 200°C, the sensor showed a response value of 237 for 100 ppm TEA with a very low detection limit (50 ppb, 1.2) and much faster response/recovery time (47 s/12 s). The performance improvement is due to changes in the potential barrier at the Rh<sub>2</sub>O<sub>3</sub> and Fe<sub>2</sub>O<sub>3</sub>/LaFeO<sub>3</sub> interface, enhancing charge transfer. Additionally, based on first principles calculations, this work examines the adsorption energy and possible adsorption configurations of adsorbates. The Rh element acts as a negative charge center, which facilitates gas adsorption and reactions on the surface via a spillover effect. This study offers a promising candidate for TEA sensing and theoretical insights into high-performance sensor development.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"438 ","pages":"Article 137791"},"PeriodicalIF":8.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasensitive electrochemical sensor for monitoring uric acid in sweat based on hexagonally-structured Co2CrMnFeNi high-entropy alloy nanosheets with exceptional catalytic performance

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-11 DOI: 10.1016/j.snb.2025.137790

Zhang Qingqing , Li Ruiyi , Yang Yongqiang , Li Zaijun

The limited sensitivity and selectivity of electrochemical sensors restrict their practical applications in uric acid analysis in human sweat. This study introduces an electrochemical sensor based on hexagonal Co₂CrMnFeNi high-entropy alloy nanosheets (Co₂CrMnFeNi) for uric acid detection. The Co₂CrMnFeNi was synthesized using a strong coordination method with arginine and serine-functionalized and boron-doped graphene quantum dots (RSB-GQD). The introduction of RSB-GQD significantly improves the homogenization, atomization, morphology control and alloying of metal elements. The resulting Co₂CrMnFeNi exhibits a hexagonal sheet structure with homogeneous elemental distribution, small size of 252 ± 1.4 nm, double FCC phase and RSB-GQD surface modification. The combination of Cr, Mn, Fe, Co with Ni elements facilitates the reconstruction of electronic structure, leading to a cobalt-rich phase, while RSB-GQD modification improves the affinity for polar electrolytes. This integration results in an electrocatalytic activity over 3.98 times greater than that of gold nanoparticles. The sensor demonstrates a sensitive response to uric acid, with current at 0.416 V increasing linearly from 0.05 to 500 μM, achieving a detection limit of 8.5 × 10⁻⁹ M. The proposed method offers significant advantages in sensitivity, selectivity, wide linear range, and repeatability for detecting uric acid in human sweat.

{"title":"Ultrasensitive electrochemical sensor for monitoring uric acid in sweat based on hexagonally-structured Co2CrMnFeNi high-entropy alloy nanosheets with exceptional catalytic performance","authors":"Zhang Qingqing , Li Ruiyi , Yang Yongqiang , Li Zaijun","doi":"10.1016/j.snb.2025.137790","DOIUrl":"10.1016/j.snb.2025.137790","url":null,"abstract":"<div><div>The limited sensitivity and selectivity of electrochemical sensors restrict their practical applications in uric acid analysis in human sweat. This study introduces an electrochemical sensor based on hexagonal Co₂CrMnFeNi high-entropy alloy nanosheets (Co₂CrMnFeNi) for uric acid detection. The Co₂CrMnFeNi was synthesized using a strong coordination method with arginine and serine-functionalized and boron-doped graphene quantum dots (RSB-GQD). The introduction of RSB-GQD significantly improves the homogenization, atomization, morphology control and alloying of metal elements. The resulting Co₂CrMnFeNi exhibits a hexagonal sheet structure with homogeneous elemental distribution, small size of 252 ± 1.4 nm, double FCC phase and RSB-GQD surface modification. The combination of Cr, Mn, Fe, Co with Ni elements facilitates the reconstruction of electronic structure, leading to a cobalt-rich phase, while RSB-GQD modification improves the affinity for polar electrolytes. This integration results in an electrocatalytic activity over 3.98 times greater than that of gold nanoparticles. The sensor demonstrates a sensitive response to uric acid, with current at 0.416 V increasing linearly from 0.05 to 500 μM, achieving a detection limit of 8.5 × 10⁻⁹ M. The proposed method offers significant advantages in sensitivity, selectivity, wide linear range, and repeatability for detecting uric acid in human sweat.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"438 ","pages":"Article 137790"},"PeriodicalIF":8.0,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143822925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An ultrasensitive electrochemical sensor based on nanoporous molecularly imprinted polymer film for triphenyl phosphate detection

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-10 DOI: 10.1016/j.snb.2025.137776

Mengqi Li , Rui Jiang , Ya Sun , Yongxin Song

As a representative of widely used flame retardant, triphenyl phosphate (TPhP) has been regarded as an emerging environmental contaminant of health concern. In this study, an ultrasensitive disposable screen-printed electrode (SPE) electrochemical sensor has been developed based on covering the electrode with a nanoporous molecularly imprinted polymer (NPMIP) film for detecting TPhP. The mechanism of the sensor was revealed by analyzing the electrokinetic motion of the redox probe inside the nanochannel of the NPMIP film. Following optimization, the sensor was found to be rapid with the detection time of approximately 35 min, showing a linear relationship to the logarithmic concentration of TPhP between 1 fM and 1 µM (R² = 0.99) and low limits of detection and quantification (LOD = 0.3 fM, LOQ = 0.9 fM). Moreover, the sensor presented more than four times better recognition of TPhP than its non-imprinted recognition. In addition, an excellent adaptability of the sensor in analyzing real samples was indicated, with recovery of 100–106 % for lake water and 92–109 % for sea water. The presented analytical approach possesses the advantages of high sensitivity, easy fabrication and low cost, which could be further developed as a portable device for onsite environmental monitoring.

{"title":"An ultrasensitive electrochemical sensor based on nanoporous molecularly imprinted polymer film for triphenyl phosphate detection","authors":"Mengqi Li , Rui Jiang , Ya Sun , Yongxin Song","doi":"10.1016/j.snb.2025.137776","DOIUrl":"10.1016/j.snb.2025.137776","url":null,"abstract":"<div><div>As a representative of widely used flame retardant, triphenyl phosphate (TPhP) has been regarded as an emerging environmental contaminant of health concern. In this study, an ultrasensitive disposable screen-printed electrode (SPE) electrochemical sensor has been developed based on covering the electrode with a nanoporous molecularly imprinted polymer (NPMIP) film for detecting TPhP. The mechanism of the sensor was revealed by analyzing the electrokinetic motion of the redox probe inside the nanochannel of the NPMIP film. Following optimization, the sensor was found to be rapid with the detection time of approximately 35 min, showing a linear relationship to the logarithmic concentration of TPhP between 1 fM and 1 µM (R<sup>2</sup> = 0.99) and low limits of detection and quantification (LOD = 0.3 fM, LOQ = 0.9 fM). Moreover, the sensor presented more than four times better recognition of TPhP than its non-imprinted recognition. In addition, an excellent adaptability of the sensor in analyzing real samples was indicated, with recovery of 100–106 % for lake water and 92–109 % for sea water. The presented analytical approach possesses the advantages of high sensitivity, easy fabrication and low cost, which could be further developed as a portable device for onsite environmental monitoring.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"438 ","pages":"Article 137776"},"PeriodicalIF":8.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accurate voltammetric analysis of As(III) in cooked rice: A true reflection of actual human intake

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-10 DOI: 10.1016/j.snb.2025.137783

Yuan-Fan Yang , Zi-Hao Liu , Li-Jun Tang , Zhi-Wei Gao , Meng Yang , Zong-Yin Song , Pei-Hua Li , Shi-Hua Chen , Xing-Jiu Huang

Rice is one of the significant sources through which the human body absorbs arsenic(As) from the environment over extended periods in Asia, in addition to drinking water. In addition, owing to its distinct biological characteristics, rice has a notable capacity to absorb As from contaminated soil and water. Current detection methods often necessitate strong acid and high-temperature pretreatment of rice, which may lead to the oxidation of As(III) to As(V), thereby failing to accurately represent the actual intake of As(III) by humans. In this study, we employed a commonly used cooking method in daily life and directly analyzed rice soup via electrochemical techniques to most accurately reflect the actual intake of As(III) by humans. We developed a sensor based on P doped Fe-Co nanobox modified glassy carbon electrodes for precise and highly sensitive detection of As(III) in rice. This sensor demonstrated a sensitivity of 2.39 µA/ppb and achieved a detection limit as low as 0.06 ppb. Furthermore, it exhibited excellent stability and reproducibility. Additionally, through X-ray photoelectron spectroscopy (XPS) and X-ray absorption fine structure (XAFS) studies, we have demonstrated that electrons are transferred from Fe/Co sites to As(III) via the "electron bridge" formed by P atoms, enabling highly sensitive detection of As(III) in rice. Our findings present a novel approach for the development of a sensor at the accurate electrochemical detection of heavy metal ions in agricultural products and hold significant potential for future applications in engineering detection.

{"title":"Accurate voltammetric analysis of As(III) in cooked rice: A true reflection of actual human intake","authors":"Yuan-Fan Yang , Zi-Hao Liu , Li-Jun Tang , Zhi-Wei Gao , Meng Yang , Zong-Yin Song , Pei-Hua Li , Shi-Hua Chen , Xing-Jiu Huang","doi":"10.1016/j.snb.2025.137783","DOIUrl":"10.1016/j.snb.2025.137783","url":null,"abstract":"<div><div>Rice is one of the significant sources through which the human body absorbs arsenic(As) from the environment over extended periods in Asia, in addition to drinking water. In addition, owing to its distinct biological characteristics, rice has a notable capacity to absorb As from contaminated soil and water. Current detection methods often necessitate strong acid and high-temperature pretreatment of rice, which may lead to the oxidation of As(III) to As(V), thereby failing to accurately represent the actual intake of As(III) by humans. In this study, we employed a commonly used cooking method in daily life and directly analyzed rice soup via electrochemical techniques to most accurately reflect the actual intake of As(III) by humans. We developed a sensor based on P doped Fe-Co nanobox modified glassy carbon electrodes for precise and highly sensitive detection of As(III) in rice. This sensor demonstrated a sensitivity of 2.39 µA/ppb and achieved a detection limit as low as 0.06 ppb. Furthermore, it exhibited excellent stability and reproducibility. Additionally, through X-ray photoelectron spectroscopy (XPS) and X-ray absorption fine structure (XAFS) studies, we have demonstrated that electrons are transferred from Fe/Co sites to As(III) via the \"electron bridge\" formed by P atoms, enabling highly sensitive detection of As(III) in rice. Our findings present a novel approach for the development of a sensor at the accurate electrochemical detection of heavy metal ions in agricultural products and hold significant potential for future applications in engineering detection.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"438 ","pages":"Article 137783"},"PeriodicalIF":8.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical aptasensor based on highly conductive rod-shaped Ce-MOF@AuPd NFs and high-efficiency 3D DNA walker for the detection of acrylamide

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2025-04-10 DOI: 10.1016/j.snb.2025.137780

Linjing Duan , Huali Jin , Yang Wen , Min Wei , Rui Guo , Zhiguang Suo , Keshavan Niranjan

As a food and environmental contaminant having harmful qualities like neurotoxicity and carcinogenicity, acrylamide (AA) is a risk to human health. In this study, Ce-MOF@AuPd NFs were utilized as electrode modification materials to develop a signal-off electrochemical aptasensor in combination with Mg²⁺-dependent DNAzyme-driven 3D DNA walker and triplex molecular switch (THMS) for the sensitive detection of AA. Ce-MOF@AuPd NFs exhibit an expansive surface area and enhanced conductive properties, enabling the binding of substantial DNA sequences and speeding up electron transfer. The Mg²⁺-dependent DNAzyme-driven 3D DNA walker improves the nucleic acid shearing efficiency with excellent signal amplification performance, and combines with the DNA-specific structure THMS to further boost sensor sensitivity. The sensor also demonstrated excellent specificity, reproducibility, repeatability, and stability, along with achieving satisfactory recovery rates when tested on potato chips and coffee samples. The electrochemical aptasensor offers strong application prospects for AA detection in food, according to the results.

{"title":"Electrochemical aptasensor based on highly conductive rod-shaped Ce-MOF@AuPd NFs and high-efficiency 3D DNA walker for the detection of acrylamide","authors":"Linjing Duan , Huali Jin , Yang Wen , Min Wei , Rui Guo , Zhiguang Suo , Keshavan Niranjan","doi":"10.1016/j.snb.2025.137780","DOIUrl":"10.1016/j.snb.2025.137780","url":null,"abstract":"<div><div>As a food and environmental contaminant having harmful qualities like neurotoxicity and carcinogenicity, acrylamide (AA) is a risk to human health. In this study, Ce-MOF@AuPd NFs were utilized as electrode modification materials to develop a signal-off electrochemical aptasensor in combination with Mg<sup>2</sup><sup>+</sup>-dependent DNAzyme-driven 3D DNA walker and triplex molecular switch (THMS) for the sensitive detection of AA. Ce-MOF@AuPd NFs exhibit an expansive surface area and enhanced conductive properties, enabling the binding of substantial DNA sequences and speeding up electron transfer. The Mg<sup>2+</sup>-dependent DNAzyme-driven 3D DNA walker improves the nucleic acid shearing efficiency with excellent signal amplification performance, and combines with the DNA-specific structure THMS to further boost sensor sensitivity. The sensor also demonstrated excellent specificity, reproducibility, repeatability, and stability, along with achieving satisfactory recovery rates when tested on potato chips and coffee samples. The electrochemical aptasensor offers strong application prospects for AA detection in food, according to the results.</div></div>","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"438 ","pages":"Article 137780"},"PeriodicalIF":8.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0