Sensors and Actuators B: Chemical最新文献_第8页

Gold nanoprism enhanced SERS aptasensor for simultaneous detection of thrombin and VEGF 同时检测凝血酶和血管内皮生长因子的金纳米光栅增强型 SERS 光传感器

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2024-10-19 DOI: 10.1016/j.snb.2024.136811

Sensitive detection of disease-specific biomarkers with high accuracy is crucial for early diagnosis, therapeutic monitoring, and understanding underlying pathological mechanisms. Traditional methods, such as immunohistochemistry and enzyme-linked immunosorbent assays (ELISA), face limitations due to the complex and expensive production of antibodies. In this context, aptamers, short oligonucleotides with advantages like easy synthesis, low cost, high specificity, and stability, have emerged as promising alternatives for biomolecular sensing. In this study, we introduce a surface-enhanced Raman spectroscopy (SERS) aptasensor for the multiplexed detection of human α-thrombin and vascular endothelial growth factor (VEGF-165). By leveraging aptamer-based biorecognition elements, our approach capitalizes on the small gaps created by aptamers, generating intense electromagnetic hotspots that significantly amplify the SERS signal. This enables simultaneous detection of human α-thrombin and VEGF-165 with remarkable sensitivity (100 fM and 1 pM, respectively). Notably, we also employ a digital protocol for analyzing the obtained vibrational spectra, marking the first-time utilization of this method for such aptasensors and offering precise quantification even at ultralow concentration regimes. We envision the reported aptasensor provides a roadmap for developing superior aptamer-based spectroscopical tools for a wide range of applications in biology and medicine.

高灵敏度地检测疾病特异性生物标志物对于早期诊断、治疗监测和了解潜在病理机制至关重要。传统的方法，如免疫组织化学和酶联免疫吸附试验（ELISA），由于抗体的生产复杂且昂贵而受到限制。在这种情况下，具有易合成、低成本、高特异性和稳定性等优点的短寡核苷酸--aptamers--已成为生物分子传感的有前途的替代品。在本研究中，我们介绍了一种表面增强拉曼光谱（SERS）适配体传感器，用于多重检测人α-凝血酶原和血管内皮生长因子（VEGF-165）。通过利用基于适配体的生物识别元件，我们的方法利用了适配体产生的微小间隙，产生了显著放大 SERS 信号的强电磁热点。这样就能同时检测人α-凝血酶原和血管内皮生长因子-165，灵敏度极高（分别为 100 fM 和 1 pM）。值得注意的是，我们还采用了数字协议来分析所获得的振动光谱，这标志着这种方法首次用于此类灵敏传感器，即使在超低浓度范围内也能精确定量。我们认为，报告中的适配体传感器为开发基于适配体的卓越光谱工具提供了路线图，可广泛应用于生物学和医学领域。

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引用次数: 0

ONOO- regulating ICT of mitochondria-targeted photosensitizers to enhance Type-I photochemical reactions 线粒体靶向光敏剂的 ONOO- 调节 ICT，以增强 I 型光化学反应

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2024-10-19 DOI: 10.1016/j.snb.2024.136810

The photochemical reaction efficiency of ICT-based Type-I photosensitizers can be effectively enhanced by the regulation of electron-donating and electron-withdrawing groups in the lesion area. Based on this strategy, a mitochondria-targeted photosensitizer (TPA-NH₂-B) was designed and synthesized in this study, using benzeneboronic acid pinacol ester group as recognition group for ONOO^-. Such molecular structure exhibits significant ICT change before and after responding to ONOO^-. TPA-NH₂-B with weaker ICT effect rapidly responds to ONOO^- within 15 s and form the reaction product TPA-NH₂ with strong ICT effect. The response process for ONOO^- is accompanied by the implementation of advanced ICT. Electron paramagnetic resonance experiments and spectrum assay with DHR123 showed an increase in ROS production after TPA-NH₂-B reacted with ONOO^-. Meanwhile, TCNQ experiment results show that the quenching constant increased by 5.7-fold after responding to ONOO^-, indicating that the efficiency of electron transfer during Type-I PDT process has been enhanced. In live cells, the response process of TPA-NH₂-B to ONOO^- has also been confirmed. TPA-NH₂-B showed high mitochondria targeting, good phototoxicity and low dark toxicity. Finally, tumor experiments validated that TPA-NH₂-B efficiently generates ROS under light irradiation, leading to cell death and inhibiting tumor growth. The above experiments prove that photosensitizers based on ICT mechanisms can adjust the efficiency of photochemical reactions by regulating the efficiency of ICT.

通过调节病变区的供电子基团和吸电子基团，可以有效提高基于ICT的I型光敏剂的光化学反应效率。基于这一策略，本研究以苯硼酸频哪醇酯基团作为ONOO-的识别基团，设计合成了一种线粒体靶向光敏剂（TPA-NH2-B）。这种分子结构在响应 ONOO- 前后表现出明显的 ICT 变化。ICT 作用较弱的 TPA-NH2-B 能在 15 秒内迅速与 ONOO- 发生反应，生成 ICT 作用较强的反应产物 TPA-NH2。对 ONOO- 的反应过程伴随着高级 ICT 的实施。用 DHR123 进行的电子顺磁共振实验和光谱分析显示，TPA-NH2-B 与 ONOO- 反应后，ROS 生成增加。同时，TCNQ 实验结果表明，与 ONOO- 反应后，淬灭常数增加了 5.7 倍，表明 I 型 PDT 过程中的电子传递效率得到了提高。在活细胞中，TPA-NH2-B 对 ONOO- 的反应过程也得到了证实。TPA-NH2-B 的线粒体靶向性强，光毒性好，暗毒性低。最后，肿瘤实验验证了 TPA-NH2-B 在光照射下能有效产生 ROS，导致细胞死亡并抑制肿瘤生长。上述实验证明，基于 ICT 机制的光敏剂可以通过调节 ICT 的效率来调节光化学反应的效率。

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引用次数: 0

Enhancing sensitivity in fluorescence measurements across an ultra-wide concentration range through optimizing combined-segments strategy 通过优化组合段策略提高超宽浓度范围荧光测量的灵敏度

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2024-10-18 DOI: 10.1016/j.snb.2024.136788

Fluorescence measurement technology is crucial in analytical instrumentation. However, when applied in fields such as environmental science and medical research for quantification purposes, it requires prior estimation of sample concentration and sample dilution to ensure measurement precision. This requirement presents challenge for its broader application in online fluorescence sensing. This study addresses the challenge of achieving high-precision measurements across an ultra-wide concentration range, which is critical for applications ranging from environmental monitoring to clinical diagnostics. The binary segmentation method can initially achieve the goal of ultra-wide range fluorescence measurement, but it fails to maintain sensitivity and precision across the entire range, with relative errors exceeding

\pm 15 %

. Therefore, we propose an optimizing combined-segments strategy, which examines the effects of adjusting the fluorescence reception position, range, and region interval parameters of the fluorescence reception probes on measurement sensitivity limits. This strategy aims to find the optimal measurement sensitivity limit (

{l m}_{opt}

) and the best combined-segments configuration based on more than one quantitative relationship curves. Experimental results demonstrate that within a concentration range

20

times broader than the linear range, it can effectively maintain relative errors within

\pm 5 % .

This enhances the applicability of fluorescence sensing techniques in various practical settings and advances online and direct fluorescence measurement technologies across an ultra-wide concentration range.

荧光测量技术在分析仪器中至关重要。然而，当应用于环境科学和医学研究等领域进行定量时，需要事先估计样品浓度和稀释样品，以确保测量精度。这一要求为在线荧光传感技术的广泛应用带来了挑战。本研究解决了在超宽浓度范围内实现高精度测量的难题，这对于从环境监测到临床诊断的各种应用都至关重要。二元分割方法最初可以实现超宽范围荧光测量的目标，但它无法在整个范围内保持灵敏度和精度，相对误差超过 ±15%。因此，我们提出了优化组合分段策略，研究调整荧光接收探头的荧光接收位置、范围和区域间隔参数对测量灵敏度限制的影响。该策略旨在根据多条定量关系曲线找到最佳测量灵敏度极限（lmopt）和最佳组合分段配置。实验结果表明，在比线性范围宽 20 倍的浓度范围内，它能有效地将相对误差保持在 ±5% 以内。这提高了荧光传感技术在各种实际环境中的适用性，并推动了超宽浓度范围内在线和直接荧光测量技术的发展。

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引用次数: 0

Tailored controllable bifunctional fluorescent-magnetic nanobeads for new generation fast-response immunosensor with dual-signal amplification 量身定制的可控双功能荧光磁性纳米吸附剂，用于新一代双信号放大快速反应免疫传感器

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2024-10-18 DOI: 10.1016/j.snb.2024.136804

Immunosensors based on the fluorescence-linked immunosorbent assay (FLISA) platform are now widely used; however, the long-term response and lower sensitivity limited their development in clinical applications. In this study, we first tailored bifunctional magnetic nanoparticle (MNP)-quantum dot (QD) nanobeads (MQBs) with rapid magnetic response and strong fluorescence intensity for the FLISA platform to construct a fast-response immunosensor. Under optimal conditions, we constructed a dual-signal amplification immunosensor based on an MQB-driven FLISA platform for a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen. We achieved a 29-fold increase in fluorescence intensity over the QD nanobeads (SiO₂/QD/SiO₂, QBs). Compared with the conventional FLISA platform-based QBs, the sensitivity of the developed immunosensor increased 63-fold to 7.50 pg/mL in half the detection time of common FLISA (only 30 min). More important, the test results of this dual-signal amplification immunosensor showed good specificity and accuracy. This novel MQB magnetic-driven FLISA platform with high throughput, as a proof of concept, effectively improved sensitivity and shortened reaction time. As a result, it is expected to have significant potential in the field of point-of-care testing.

基于荧光联免疫吸附测定（FLISA）平台的免疫传感器目前已得到广泛应用，但其长期响应和较低的灵敏度限制了其在临床应用中的发展。在本研究中，我们首先为 FLISA 平台定制了具有快速磁响应和强荧光强度的双功能磁性纳米颗粒（MNP）-量子点（QD）纳米吸附珠（MQBs），构建了一种快速响应免疫传感器。在最佳条件下，我们构建了一种基于 MQB 驱动 FLISA 平台的双信号放大免疫传感器，用于检测严重急性呼吸系统综合征冠状病毒 2（SARS-CoV-2）抗原。与 QD 纳米吸附珠（SiO2/QD/SiO2，QBs）相比，我们的荧光强度提高了 29 倍。与基于传统 FLISA 平台的 QBs 相比，所开发的免疫传感器的灵敏度提高了 63 倍，达到 7.50 pg/mL，而检测时间仅为普通 FLISA 的一半（30 分钟）。更重要的是，这种双信号放大免疫传感器的检测结果显示出良好的特异性和准确性。作为概念验证，这种新型 MQB 磁驱动 FLISA 高通量平台有效提高了灵敏度，缩短了反应时间。因此，它有望在护理点检测领域大显身手。

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引用次数: 0

A high-performance, flexible, and dual-modal humidity-piezoelectric sensor without mutual interference 一种高性能、灵活、无相互干扰的双模式湿度压电传感器

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2024-10-18 DOI: 10.1016/j.snb.2024.136778

High-performance, flexible, and multifunctional humidity-piezoelectric sensors are applicable to respiratory pattern detection, man-machine motion distinction, and dual-modal speech recognition. However, signal disturbances between the humidity and pressure signals remain, and interference from the external environment such as temperature further results in signal vibrations. In this study, a high-performance, flexible, and dual-modal humidity-piezoelectric sensor is introduced to achieve accurate and stable sensing signals by fully prohibiting mutual interference towards simultaneous humidity and pressure stimuli. It is first designed using a high-performance humidity-sensing unit constructed by polyacrylate sodium (PAAS)/carbon nanotube (CNT) film and a piezoelectric sensing unit formed by polyvinylidene fluoride (PVDF)/CNT nanofiber film. The patterned PAAS/CNT unit exhibits a resistance change of 1100 % in relative humidity of 23 %–98 %, minor hysteresis, favorable durability, and negligible pressure interference. The PVDF/CNT piezoelectric unit demonstrates a sensitivity of 0.11 V/kPa in a wide pressure range of 3–115 kPa, good linearity, high durability, negligible humidity and temperature interference, and fully prohibited signal noise. Moreover, the high-performance, flexible, dual-modal humidity-piezoelectric sensors display significant applications for man-machine motion detection, human gestures and object distinction, and dual-modal speech recognition.

高性能、灵活、多功能的湿压电传感器适用于呼吸模式检测、人机运动区分和双模态语音识别。然而，湿度信号和压力信号之间仍然存在信号干扰，外部环境（如温度）的干扰会进一步导致信号振动。本研究介绍了一种高性能、灵活的双模态湿度压电传感器，通过完全避免同时受到湿度和压力刺激时的相互干扰，实现精确稳定的传感信号。首先设计了由聚丙烯酸钠（PAAS）/碳纳米管（CNT）薄膜构成的高性能湿度传感单元和由聚偏氟乙烯（PVDF）/CNT 纳米纤维薄膜构成的压电传感单元。图案化 PAAS/CNT 单元在相对湿度为 23%-98% 的条件下电阻变化率为 1100%，滞后小，耐用性好，压力干扰可忽略不计。PVDF/CNT 压电单元在 3-115 kPa 的宽压力范围内具有 0.11 V/kPa 的灵敏度、良好的线性度、高耐用性、可忽略的湿度和温度干扰以及完全禁止的信号噪声。此外，这种高性能、灵活的双模态湿度压电传感器在人机运动检测、人的手势和物体区分以及双模态语音识别方面具有重要的应用前景。

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引用次数: 0

Primer exchange reaction activation of CRISPR/Cas12a system to construct a versatile and label-free electrochemical sensing platform 激活 CRISPR/Cas12a 系统的引物交换反应，构建多功能无标记电化学传感平台

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2024-10-18 DOI: 10.1016/j.snb.2024.136791

The detection of antibiotics and pesticide residues is extremely important to maintain food safety. In this work, a versatile and label-free electrochemical biosensing platform was developed to sensitively detect antibiotics and pesticide residues using primer exchange reaction (PER) signal amplification technology integrated with CRISPR/Cas12a system. Initially, single-stranded DNA (ssDNA, S1) rich in guanine was pre-modified on the electrode surface to capture hemin and form G-quadruplex/hemin complexes, achieving strong electrochemical signal output. When the target was available, it could bind to the aptamer and release the hairpin template of PER, and with the help of DNA polymerase, the short primers pre-modified on the electrode surface were extended into long ssDNA through two PER cycles. The long ssDNA generated by PER circuit contained multiple binding sites of CRISPR/Cas12a, which activated CRISPR/Cas12a system and rapidly cleaved S1 on the electrode surface, preventing the formation of G-quadruplex/hemin complexes, resulting in significantly weakened electrochemical signal and achieving label-free and sensitive detection of the target. Benefiting from the programmability of DNA, universal detection of ampicillin, kanamycin, and acetamiprid was achieved by simply changing the hairpin substrate of PER, with detection limits of 0.33 pM, 0.38 pM, and 0.78 pM (S/N = 3), respectively. Meaningfully, the sensing platform successfully performed the detection of ampicillin in milk and livestock wastewater samples, supplying a new electrochemical analysis method with respect to accurate and reliable detection of actual samples.

抗生素和农药残留的检测对维护食品安全极为重要。本研究开发了一种多功能、无标记的电化学生物传感平台，利用引物交换反应（PER）信号放大技术与 CRISPR/Cas12a 系统集成，灵敏地检测抗生素和农药残留。最初，富含鸟嘌呤的单链 DNA（ssDNA，S1）被预先修饰在电极表面，以捕获海明并形成 G-四链/海明复合物，从而实现强大的电化学信号输出。当目标物可用时，它可以与适配体结合并释放出 PER 的发夹模板，在 DNA 聚合酶的帮助下，电极表面预修饰的短引物通过两个 PER 循环延伸成长 ssDNA。PER回路产生的长ssDNA含有多个CRISPR/Cas12a的结合位点，激活了CRISPR/Cas12a系统，迅速裂解电极表面的S1，阻止了G-四链体/hemin复合物的形成，导致电化学信号明显减弱，实现了目标物的无标记灵敏检测。得益于 DNA 的可编程性，只需改变 PER 的发夹底物，就能实现对氨苄西林、卡那霉素和啶虫脒的通用检测，检测限分别为 0.33 pM、0.38 pM 和 0.78 pM（S/N = 3）。该传感平台成功检测了牛奶和畜禽废水样品中的氨苄西林，为准确可靠地检测实际样品提供了一种新的电化学分析方法。

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引用次数: 0

Fabrication and Application of ZIF-8 Derived In2O3-ZnO Nanocomposites for Ultra-Sensitive NO2 Sensing under UV Irradiation at Room Temperature 室温紫外线照射下用于超灵敏二氧化氮传感的 ZIF-8 衍生 In2O3-ZnO 纳米复合材料的制备与应用

IF 8.4 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2024-10-18 DOI: 10.1016/j.snb.2024.136802

Xingyu Kang, Jianhui Lv, Lei Han, Bin Huang, Huizhang Zhao, Dong Wang, Hairui Fang

Nitrogen oxide (NO₂) is common by-products of industrial production, posing significant health risks to workers. Therefore, real-time monitoring of NO₂ is crucial. In this study, innovative In₂O₃-ZnO nano-materials were prepared by combining In₂O₃ with ZIF-8 via electrospinning, for detecting trace amounts of NO₂ at room temperature (RT). Compared to pure In₂O₃, the In₂O₃-ZnO nano-materials (V2) with a 10% weight ratio of ZIF-8 exhibited a significantly higher response (389.99) and a faster response time (16.9 s) to NO₂ under ultraviolet (UV) irradiation at RT. The superior gas sensing performance of the V2 nano-materials is attributed to the formation of n-n heterojunctions, enhanced optical absorption, increased oxygen vacancy ratio, and excellent dispersion among the nano-materials. The results demonstrate that V2 nano-materials can detect NO₂ at parts-per-billion (ppb) levels at RT, making them suitable for monitoring trace amounts of NO₂ in open environments. Furthermore, a portable real-time NO₂ monitoring device was constructed using V2 as the sensing material, an LM358-based amplifier circuit for data acquisition, an STM32F103C8T6 microcontroller as the main control unit, and a buzzer as an alarm device. Compared to devices using a helical Ni-Cr wire as an indirect heat source, this integrated NO₂ detection device offers real-time alarms with lower energy consumption. This application not only enhances worker health and safety in industrial environments but also demonstrates higher energy efficiency in practical application.

氮氧化物（NO2）是工业生产中常见的副产品，对工人的健康构成重大威胁。因此，对二氧化氮进行实时监测至关重要。本研究通过电纺丝将 In2O3 与 ZIF-8 结合在一起，制备了创新的 In2O3-ZnO 纳米材料，用于在室温（RT）下检测痕量 NO2。与纯 In2O3 相比，含有 10% 重量比 ZIF-8 的 In2O3-ZnO 纳米材料（V2）在室温紫外线（UV）照射下对 NO2 的响应明显更高（389.99），响应时间更快（16.9 秒）。V2 纳米材料优异的气体传感性能归功于 n-n 异质结的形成、光吸收的增强、氧空位率的提高以及纳米材料之间良好的分散性。研究结果表明，V2 纳米材料能在实时环境中检测到十亿分之一（ppb）水平的二氧化氮，因此适用于监测开放环境中的痕量二氧化氮。此外，还利用 V2 作为传感材料、基于 LM358 的放大电路（用于数据采集）、STM32F103C8T6 微控制器作为主控单元以及蜂鸣器作为报警装置，构建了一个便携式 NO2 实时监测装置。与使用螺旋镍铬丝作为间接热源的设备相比，这种集成式二氧化氮检测设备能以更低的能耗提供实时报警。这一应用不仅提高了工业环境中工人的健康和安全，而且在实际应用中体现了更高的能效。

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引用次数: 0

Enzymatic reaction and competitive prereduction enable label-free and separation-free inductively coupled plasma mass spectrometry detection of leukemia cells 酶促反应和竞争性预还原实现了对白血病细胞的无标记、无分离电感耦合等离子体质谱检测

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2024-10-18 DOI: 10.1016/j.snb.2024.136772

The enzyme terminal deoxynucleotidyl transferase (TdT) exhibits significant activity in various leukemia cells, including human acute lymphoblastic leukemia cells (CCRF). It demonstrates substantial potential as a diagnostic biomarker for acute lymphoblastic leukemia (ALL). Herein, a highly sensitive method for the homogeneous quantification of TdT and CCRF cells, without the need for labeling or separation, was established based on inductively coupled plasma mass spectrometry (ICP-MS). The simple sensor relied on the TdT-induced polymerization process, lengthening the DNA strand and producing large amounts of the by-product phosphates of pyrophosphate (PPi). The PPi formed complexes with Cu²⁺, altering its morphology in homogeneous systems. The remaining Cu²⁺ competed with Hg²⁺ in consuming ascorbic acid (AA) through the pre-reduction of Hg²⁺, affecting the ICP-MS signal of Hg²⁺. Under optimal conditions, this approach exhibited excellent specificity and ultrahigh sensitivity, with limits of detection (LODs) as low as 0.05 U/L for TdT and 5 cells/mL for CCRF cells, respectively. The proposed method offers significant advantages, including high sensitivity and precision, operation at room temperature without the involvement of proteases, and the ability to accurately quantify in complex biological matrices. Consequently, the proposed method shows great promise as a valuable tool for TdT-related biological research and leukemia diagnosis.

末端脱氧核苷酸转移酶（TdT）在包括人类急性淋巴细胞白血病细胞（CCRF）在内的各种白血病细胞中表现出显著的活性。它作为急性淋巴细胞白血病（ALL）诊断生物标记物的潜力巨大。在此，我们建立了一种基于电感耦合等离子体质谱法（ICP-MS）的高灵敏度方法，无需标记或分离，即可对 TdT 和 CCRF 细胞进行均匀定量。这种简单的传感器依赖于 TdT 诱导的聚合过程，该过程会延长 DNA 链并产生大量焦磷酸磷酸盐（PPi）副产品。PPi 与 Cu²⁺ 形成复合物，改变了其在均相系统中的形态。剩余的 Cu²⁺ 通过预先还原 Hg²⁺ 与 Hg²⁺ 竞争消耗抗坏血酸（AA），从而影响 Hg²⁺ 的 ICP-MS 信号。在最佳条件下，该方法具有极佳的特异性和超高灵敏度，对 TdT 和 CCRF 细胞的检测限分别低至 0.05 U/L 和 5 cells/mL。所提出的方法具有显著的优势，包括灵敏度高、精确度高、可在室温下操作而无需蛋白酶的参与，以及能够在复杂的生物基质中准确定量。因此，该方法有望成为 TdT 相关生物学研究和白血病诊断的重要工具。

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引用次数: 0

A reaction-based fluorescent probe for MGO visualization and its application for in vivo imaging and drought stress monitoring in Arabidopsis thaliana 基于反应的 MGO 可视化荧光探针及其在拟南芥体内成像和干旱胁迫监测中的应用

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2024-10-18 DOI: 10.1016/j.snb.2024.136806

Methylglyoxal (MGO) is a highly reactive metabolic byproduct present in both plants and animals, closely linked to various disease developments. And fluorescent probes, with their high sensitivity, non-invasiveness, and real-time visualization, are ideal for MGO monitoring. While most existing MGO fluorescence probes are designed for applications in cells and animals, none have been applied to studying MGO levels in plants. To address this gap, we herein present a coumarin derivative fluorescent probe, CNDS, with exceptional selectivity and sensitivity to MGO. Our research demonstrated that CNDS can sensitively and selectively detect MGO in solution and has been successfully employed for imaging endogenous and exogenous MGO levels in living cells, zebrafish, and Arabidopsis thaliana. Most importantly, CNDS has successfully tracked MGO levels in Arabidopsis thaliana, indicating that high MGO levels can inhibit the root growth of Arabidopsis thaliana. Additionally, we observed that MGO levels in Arabidopsis thaliana increased when subjected to drought stress, contributing to inhibited root development and shorter root lengths. The results reveal the direct impact of MGO levels on plant growth and its potential as a key marker in drought stress studies. And our findings highlight the capability of probe CNDS as a versatile tool for monitoring MGO dynamics in plant growth and development, providing significant potential for advancing the understanding of plant growth mechanisms.

甲基乙二酸（MGO）是一种存在于动植物体内的高活性代谢副产物，与各种疾病的发展密切相关。而荧光探针具有高灵敏度、非侵入性和实时可视化的特点，是监测 MGO 的理想选择。现有的大多数 MGO 荧光探针都是为细胞和动物的应用而设计的，但还没有一种用于研究植物中的 MGO 水平。为了填补这一空白，我们在本文中介绍了一种香豆素衍生物荧光探针 CNDS，它对 MGO 具有极高的选择性和灵敏度。我们的研究表明，CNDS 可以灵敏地选择性检测溶液中的 MGO，并已成功用于活细胞、斑马鱼和拟南芥中内源性和外源性 MGO 水平的成像。最重要的是，CNDS 成功跟踪了拟南芥中的 MGO 水平，表明高水平的 MGO 会抑制拟南芥根的生长。此外，我们还观察到，拟南芥在遭受干旱胁迫时，其 MGO 含量会增加，从而导致根系发育受抑制和根系长度缩短。这些结果揭示了 MGO 水平对植物生长的直接影响，以及其作为干旱胁迫研究关键标记的潜力。我们的研究结果突显了探针 CNDS 作为监测植物生长和发育过程中 MGO 动态的多功能工具的能力，为促进对植物生长机制的了解提供了巨大的潜力。

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引用次数: 0

Low Temperature Ammonia Sensor with Giant Sensitivity and Selectivity based on W@WO2.92 Internal Ohmic Contact Core-Shell Nanostructures 基于 W@WO2.92 内部欧姆接触核壳纳米结构的具有高灵敏度和选择性的低温氨气传感器

IF 8.4 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical

Pub Date : 2024-10-18 DOI: 10.1016/j.snb.2024.136792

You Wu, Weilong Chen, Xiang Long, Rui Zhai, Fangrong Qin, Zhuan Zhao, Bin Wang, Shaolin Zhang, Shusheng Pan

Ammonia (NH₃) detection is crucial for both harnessing hydrogen energy and safeguarding public health. However, the NH₃ sensors based on semiconductors is currently constrained by their necessity to operate at elevated temperatures and their limited ability to distinguish NH₃ from other gases. In this study, the high oxygen vacancy W@WO_2.92 core-shell nanostructures have been synthesized by pulsed laser ablation in water. The gas sensors based on W@WO_2.92 nanoparticles response to NH₃ with high sensitivity (|R_a-R_g|/R_a=72.6%@100 ppm, R_a and R_g represent the resistance of the material when exposed to air and the test gas, respectively.) at room temperature with detection limit of 730 ppb. Additionally, the response to NH₃ can achieve 55% even at 5℃, which plays a great role in timely detection of NH₃ during low temperature transportation. Furthermore, the sensors reveal outstanding selectivity toward NH₃ in the presence of 11 other potential interfering gases. This research presents a groundbreaking strategy, holding significant potential for the development of high-performance NH₃ sensors that operate efficiently at low temperatures.

氨气（NH3）检测对于利用氢能和保障公众健康都至关重要。然而，基于半导体的 NH3 传感器目前受限于其必须在高温下工作，以及区分 NH3 和其他气体的能力有限。本研究通过脉冲激光烧蚀法在水中合成了高氧空位的 W@WO2.92 核壳纳米结构。基于 W@WO2.92 纳米粒子的气体传感器在室温下对 NH3 具有高灵敏度（|Ra-Rg|/Ra=72.6%@100 ppm，Ra 和 Rg 分别代表材料暴露于空气和测试气体时的电阻），检测限为 730 ppb。此外，即使在 5℃，对 NH3 的响应也能达到 55%，这对于在低温运输过程中及时检测 NH3 发挥了重要作用。此外，该传感器在存在其他 11 种潜在干扰气体的情况下，对 NH3 具有出色的选择性。这项研究提出了一种开创性的策略，为开发在低温条件下高效运行的高性能 NH3 传感器提供了巨大潜力。

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