Kamer Gur, Saime Erol, E. Gunes, Serap Çi̇fçi̇li̇, Burcu K CALIK, A. Ozer, I. Demirbuken, M. Polat, Çiğdem Apaydın Kaya
Objective: The aim of this study is to evaluate the health behavior and health needs of medical and health sciences students in order to identify areas that need intervention. Materials and Methods: This descriptive study was conducted with 770 first-year university students. The data were collected with a sociodemographic questionnaire, the Youth Risk Behavior Survey and the Eating Attitudes Test. Body mass index was calculated. Results: Although, only 12.7% of the students were overweight or obese, 25.6% of the students perceived themselves to be overweight or obese. The students said that in the last week, 20.9% had breakfast 1 or 3 times, 5.7% ate no fruits at all, and 11.6% ate no vegetables at all. 48.1% of the students did not engage in any physical activity, and 83.2% spent more than 2 hours inactively in front of a screen. It was observed that 22.3% were current smokers. A propensity for eating behaviors disorder was found in 9.0%. Drinking energyboosting sports drinks 1-6 times in the last week (β: 3.286), smoking (β: 1.875) and eating few vegetable dishes in the last week (β: 0.484) were identified as factors that could be associated with a “tendency for eating behavior disorder.” Conclusion: We can conclude that nutritional issues, negative body weight perception, use of tobacco, and sedentary lifestyle are the main intervention and counselling areas for our study group.
{"title":"Health behavior and health needs of first-year medical and health sciences students","authors":"Kamer Gur, Saime Erol, E. Gunes, Serap Çi̇fçi̇li̇, Burcu K CALIK, A. Ozer, I. Demirbuken, M. Polat, Çiğdem Apaydın Kaya","doi":"10.5472/marumj.1244398","DOIUrl":"https://doi.org/10.5472/marumj.1244398","url":null,"abstract":"Objective: The aim of this study is to evaluate the health behavior and health needs of medical and health sciences students in order \u0000to identify areas that need intervention. \u0000Materials and Methods: This descriptive study was conducted with 770 first-year university students. The data were collected with \u0000a sociodemographic questionnaire, the Youth Risk Behavior Survey and the Eating Attitudes Test. Body mass index was calculated. \u0000Results: Although, only 12.7% of the students were overweight or obese, 25.6% of the students perceived themselves to be overweight \u0000or obese. The students said that in the last week, 20.9% had breakfast 1 or 3 times, 5.7% ate no fruits at all, and 11.6% ate no vegetables \u0000at all. 48.1% of the students did not engage in any physical activity, and 83.2% spent more than 2 hours inactively in front of a screen. \u0000It was observed that 22.3% were current smokers. A propensity for eating behaviors disorder was found in 9.0%. Drinking energyboosting \u0000sports drinks 1-6 times in the last week (β: 3.286), smoking (β: 1.875) and eating few vegetable dishes in the last week (β: \u00000.484) were identified as factors that could be associated with a “tendency for eating behavior disorder.” \u0000Conclusion: We can conclude that nutritional issues, negative body weight perception, use of tobacco, and sedentary lifestyle are the \u0000main intervention and counselling areas for our study group.","PeriodicalId":43341,"journal":{"name":"Marmara Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43208219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The role of health professionals working in primary care in providing services to all segments of society and in protecting and improving the health of all individuals is extremely important. The aim of this study is to determine the healthy lifestyle behaviors of primary health care workers in Karabük city center and to examine some factors that may affect the choice of a healthy lifestyle. Materials and Methods: This cross-sectional study was conducted between October-December 2019 with the participation of 334 healthcare professionals in Karabük. The questionnaire with 27 questions and the Healthy Lifestyle Behaviors Scale II (HLBS-II) were administered. Results: The median age of the health care workers participating in the study was 38.2 ± 10.6. Of the participants 53.8% were women and approximately three-quarters of the participants (70.8%) were married. In terms of occupational groups, 10.9% of the participants were physicians, 25.0% were nurses and midwives, 16.7% were health officers/technicians and 47.4% were composed of other employees. The HLBS-II total score was found to be 126.8±21.3. The HLBS-II total score were higher in women, those who defined their health status as good, university graduates those who did not smoke, had good nutrition, exercised, and were satisfied with their body appearance. Conclusion: In our study, it was found that the healthy life behavior scores of health workers were at a good level. Especially men, smokers, high school graduates, obese, those who do not pay attention to their nutrition and physical activity should be informed and be supported by healthy living behavior programs.
{"title":"Healthy life-style behaviors and related factors among Turkish primary health care professionals","authors":"B. Oral, N. Sevinc, B. Korkut","doi":"10.5472/marumj.1244431","DOIUrl":"https://doi.org/10.5472/marumj.1244431","url":null,"abstract":"Objective: The role of health professionals working in primary care in providing services to all segments of society and in protecting \u0000and improving the health of all individuals is extremely important. The aim of this study is to determine the healthy lifestyle behaviors \u0000of primary health care workers in Karabük city center and to examine some factors that may affect the choice of a healthy lifestyle. \u0000Materials and Methods: This cross-sectional study was conducted between October-December 2019 with the participation of 334 \u0000healthcare professionals in Karabük. The questionnaire with 27 questions and the Healthy Lifestyle Behaviors Scale II (HLBS-II) were \u0000administered. \u0000Results: The median age of the health care workers participating in the study was 38.2 ± 10.6. Of the participants 53.8% were women \u0000and approximately three-quarters of the participants (70.8%) were married. In terms of occupational groups, 10.9% of the participants \u0000were physicians, 25.0% were nurses and midwives, 16.7% were health officers/technicians and 47.4% were composed of other \u0000employees. The HLBS-II total score was found to be 126.8±21.3. The HLBS-II total score were higher in women, those who defined \u0000their health status as good, university graduates those who did not smoke, had good nutrition, exercised, and were satisfied with their \u0000body appearance. \u0000Conclusion: In our study, it was found that the healthy life behavior scores of health workers were at a good level. Especially men, \u0000smokers, high school graduates, obese, those who do not pay attention to their nutrition and physical activity should be informed and \u0000be supported by healthy living behavior programs.","PeriodicalId":43341,"journal":{"name":"Marmara Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48148150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: The aim of this research was to determine the changes in relationships between healthcare professional mothers and their preschool-aged children during the coronavirus disease 2019 (COVID-19) pandemic. In addition, the second objective of this study was to propose a new phenomenon that explains “being a mother as a healthcare professional” during the COVID-19 pandemic. Materials and Methods: The participants of the research were 16 healthcare professional mothers (8 doctors and 8 nurses) who had worked in intensive care units during the COVID-19 pandemic and had a preschool-aged child. The research was conducted in accordance with the phenomenological approach, one of the qualitative research designs. The research data were obtained through face-to-face interviews between the researchers and the participants using half-structured interview forms prepared by the researchers. Colaizzi’s 7-step method was used for evaluation of the data. Results: According to the research findings, the phenomenon of “being a mother as a healthcare professional” was gathered under four main themes: emotional reactions, new normal in life, difficulties that pandemic brought in life and coping strategies with these difficulties. Conclusion: The findings showed that the COVID-19 pandemic caused many changes in the lives of healthcare professional mothers and their children; these mothers and children built some emotional reactions, and they developed various strategies to overcome these emotional reactions.
{"title":"Being a mother as a healthcare professional in the COVID-19 pandemic: A qualitative study","authors":"Nesibe Gunay Molu, Sadiye Sert, Neslihan Durmuşoğlu Saltali","doi":"10.5472/marumj.1244379","DOIUrl":"https://doi.org/10.5472/marumj.1244379","url":null,"abstract":"Objective: The aim of this research was to determine the changes in relationships between healthcare professional mothers and their\u0000preschool-aged children during the coronavirus disease 2019 (COVID-19) pandemic. In addition, the second objective of this study\u0000was to propose a new phenomenon that explains “being a mother as a healthcare professional” during the COVID-19 pandemic.\u0000Materials and Methods: The participants of the research were 16 healthcare professional mothers (8 doctors and 8 nurses) who had\u0000worked in intensive care units during the COVID-19 pandemic and had a preschool-aged child. The research was conducted in\u0000accordance with the phenomenological approach, one of the qualitative research designs. The research data were obtained through\u0000face-to-face interviews between the researchers and the participants using half-structured interview forms prepared by the researchers.\u0000Colaizzi’s 7-step method was used for evaluation of the data.\u0000Results: According to the research findings, the phenomenon of “being a mother as a healthcare professional” was gathered under\u0000four main themes: emotional reactions, new normal in life, difficulties that pandemic brought in life and coping strategies with these\u0000difficulties.\u0000Conclusion: The findings showed that the COVID-19 pandemic caused many changes in the lives of healthcare professional mothers\u0000and their children; these mothers and children built some emotional reactions, and they developed various strategies to overcome\u0000these emotional reactions.","PeriodicalId":43341,"journal":{"name":"Marmara Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48767276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined their effects on MCF-7 cells. Materials and Methods: Whey proteins obtained from 6% whey powder were treated with hazelnut oil (HO) and olive oil (OO) at a protein to lipid ratio of 1:10 at 60 ̊C for 120 minutes. The protein derivatives formed with whey protein and HO or OO were applied to MCF-7 cancer cells and healthy fibroblasts. The effects of protein derivatives on cell viability, apoptosis, reactive oxygen species (ROS) production, wound healing, cell cycle phase distribution and cell cycle related proteins Akt and p21(Waf1/Cip1) expressions were investigated. Results: Cell viability decreased significantly after 24 h of incubation with WP:OO. The percentage of apoptotic or necrotic cells varied between 5-10% and no statistically significant effect was observed. There was no statistically significant difference in ROS production and colony formation between controls and WP:HO or WP:OO groups. Treatment of cells with WP:OO for 24 h significantly decreased cell migration compared to the control group. G2/M phase was significantly suppressed in WP:OO group compared to the control group. WP:OO also increased the expression of p21(Waf1/Cip1) significantly when compared with the control group. Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer treatment.
{"title":"Effect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells","authors":"F. Aksoy, A. Yılmaz, Gökhan Biçim, A. Yalçın","doi":"10.5472/marumj.1244676","DOIUrl":"https://doi.org/10.5472/marumj.1244676","url":null,"abstract":"Objective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined \u0000their effects on MCF-7 cells. \u0000Materials and Methods: Whey proteins obtained from 6% whey powder were treated with hazelnut oil (HO) and olive oil (OO) at a \u0000protein to lipid ratio of 1:10 at 60 ̊C for 120 minutes. The protein derivatives formed with whey protein and HO or OO were applied \u0000to MCF-7 cancer cells and healthy fibroblasts. The effects of protein derivatives on cell viability, apoptosis, reactive oxygen species \u0000(ROS) production, wound healing, cell cycle phase distribution and cell cycle related proteins Akt and p21(Waf1/Cip1) expressions \u0000were investigated. \u0000Results: Cell viability decreased significantly after 24 h of incubation with WP:OO. The percentage of apoptotic or necrotic cells varied \u0000between 5-10% and no statistically significant effect was observed. There was no statistically significant difference in ROS production \u0000and colony formation between controls and WP:HO or WP:OO groups. Treatment of cells with WP:OO for 24 h significantly decreased \u0000cell migration compared to the control group. G2/M phase was significantly suppressed in WP:OO group compared to the control \u0000group. WP:OO also increased the expression of p21(Waf1/Cip1) significantly when compared with the control group. \u0000Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer \u0000treatment.","PeriodicalId":43341,"journal":{"name":"Marmara Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49032803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: Our aim is to determine the levels of troponin-I and some coagulation markers (D-dimer, fibrinogen and International Normalized Ratio (INR)) in coronavirus disease 2019 (COVID-19) patients and to investigate the effects of these markers on mortality. Patients and Methods: It is planned as a descriptive, cross-sectional and analytical study. The study was conducted by retrospectively scanning the files of COVID-19 patients who applied to Inonu University Turgut Ozal Medical Center between 01.03.2020 and 31.12.2020. Levels of cardiac troponin I markers and coagulation parameters (D-dimer, fibrinogen and INR) were detected. Results: The results of a total of 1858 patients were obtained. One thousand, three hundred and twenty-six patients with only troponin I and D-dimer results (Group 1), 606 patients with only troponin I and fibrinogen results (Group 2), and 1308 patients with only troponin I and INR results (Group 3) were included. Troponin I levels were significantly higher in all patients who died. 96.6% of the patients with high D-dimer levels died in Group 1, 85.5% of the patients with high fibrinogen levels died in Group 2 and 77.3 % of the patients with high INR levels died in Group 3. Conclusion: Measurements of troponin-I and coagulation markers such as D-dimer, fibrinogen and INR can help predict clinical severity and mortality in COVID-19 patients.
{"title":"Effect of troponin I and coagulation parameters on mortality in COVID-19 patients","authors":"M. Dağ, N. Bulut, M. Taskapan","doi":"10.5472/marumj.1235703","DOIUrl":"https://doi.org/10.5472/marumj.1235703","url":null,"abstract":"Objective: Our aim is to determine the levels of troponin-I and some coagulation markers (D-dimer, fibrinogen and International Normalized Ratio (INR)) in coronavirus disease 2019 (COVID-19) patients and to investigate the effects of these markers on mortality. \u0000Patients and Methods: It is planned as a descriptive, cross-sectional and analytical study. The study was conducted by retrospectively scanning the files of COVID-19 patients who applied to Inonu University Turgut Ozal Medical Center between 01.03.2020 and 31.12.2020. Levels of cardiac troponin I markers and coagulation parameters (D-dimer, fibrinogen and INR) were detected. \u0000Results: The results of a total of 1858 patients were obtained. One thousand, three hundred and twenty-six patients with only troponin I and D-dimer results (Group 1), 606 patients with only troponin I and fibrinogen results (Group 2), and 1308 patients with only troponin I and INR results (Group 3) were included. Troponin I levels were significantly higher in all patients who died. 96.6% of the patients with high D-dimer levels died in Group 1, 85.5% of the patients with high fibrinogen levels died in Group 2 and 77.3 % of the patients with high INR levels died in Group 3. \u0000Conclusion: Measurements of troponin-I and coagulation markers such as D-dimer, fibrinogen and INR can help predict clinical severity and mortality in COVID-19 patients.","PeriodicalId":43341,"journal":{"name":"Marmara Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43327432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Miray SANCAKTAR DEMIROZ, S. Hıdıroğlu, Dilajla Oraovcanin, Merve Akbas, Annisha Condace Skinner, Sumeyye Karapinar, Ayse Sari, M. Karavuş
This study aims to evaluate the glaucoma knowledge and awareness in individuals who visit to two Family Health Centers in a district of Istanbul. Patients and Method: This descriptive study, using a three-part questionnaire consisting of 20 questions, was carried out on individuals who applied to two Family Health Centers in a district of Istanbul between May and June 2019. Data from 260 were collected through face to face interviews. Results: A total of 44 (16.9%) participants had heard the word glaucoma before, while 179 (68.8%) said that glaucoma was treatable, 78 (30%) knew about asymptomatic course glaucoma. In addition, 47(18.1%) believed that eyes with glaucoma could not be operated, 152 (58.5%) thought that blindness resulting from glaucoma was reversible. Interestingly, 167(68.7%) participants thought that routine ophthalmologic visits should be done at least once a year, whereas 222 (86.9%) consulted an ophthalmologist less than 10 times in their lifetime. Conclusion: There was no statistically significant difference between individuals with family members that have glaucoma and those that do not, when asked about glaucoma being asymptomatic, the preventability of blindness and whether or not they have heard of glaucoma before. The study findings stress the need to spread awareness about glaucoma for prevention of glaucoma related blindness.
{"title":"Glaucoma awareness in Family Health Centers","authors":"Miray SANCAKTAR DEMIROZ, S. Hıdıroğlu, Dilajla Oraovcanin, Merve Akbas, Annisha Condace Skinner, Sumeyye Karapinar, Ayse Sari, M. Karavuş","doi":"10.5472/marumj.1244706","DOIUrl":"https://doi.org/10.5472/marumj.1244706","url":null,"abstract":"This study aims to evaluate the glaucoma knowledge and awareness in individuals who visit to two Family Health Centers \u0000in a district of Istanbul. \u0000Patients and Method: This descriptive study, using a three-part questionnaire consisting of 20 questions, was carried out on individuals \u0000who applied to two Family Health Centers in a district of Istanbul between May and June 2019. Data from 260 were collected through \u0000face to face interviews. \u0000Results: A total of 44 (16.9%) participants had heard the word glaucoma before, while 179 (68.8%) said that glaucoma was treatable, \u000078 (30%) knew about asymptomatic course glaucoma. In addition, 47(18.1%) believed that eyes with glaucoma could not be operated, \u0000152 (58.5%) thought that blindness resulting from glaucoma was reversible. Interestingly, 167(68.7%) participants thought that routine \u0000ophthalmologic visits should be done at least once a year, whereas 222 (86.9%) consulted an ophthalmologist less than 10 times in \u0000their lifetime. \u0000Conclusion: There was no statistically significant difference between individuals with family members that have glaucoma and those \u0000that do not, when asked about glaucoma being asymptomatic, the preventability of blindness and whether or not they have heard of \u0000glaucoma before. The study findings stress the need to spread awareness about glaucoma for prevention of glaucoma related blindness.","PeriodicalId":43341,"journal":{"name":"Marmara Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44366073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kadriye Erdogan, N. Sanlier, Emine UTLU OZEN, S. Dilbaz, I. Kahyaoğlu, Y. Ustun
Objective: The aim of our study was to evaluate sonographic endometrial thickness succeeding the estrogen-only stage and on the day when embryo transfer (ET) occurred in artificial frozen embryo transfer (FET) cycles to delve into the effect of endometrial compaction (EC) on clinical pregnancy rate (CPR). Patients and Methods: In the first group endometrial thickness diminished when ET occurred when compared to the end of the estrogen-only phase (n:37). Endometrial thickness increased/did not alter for the second group (n:70). Demographic characteristics were recorded and the following were studied: in vitro fertilization (IVF) treatment indications, hormone levels, total antral follicle count, duration of infertility, embryo quality, embryo-fundus distance, endometrial thickness at the end of estrogen-only phase and on ET day, luteal support, CPR. Results: No significant difference occurred in CPRs (n:107). ET, on day 5 was higher in the first group (p
{"title":"Investigating the impact of endometrial compaction on clinical pregnancy rate in artificial frozen-thawed embryo transfer cycles","authors":"Kadriye Erdogan, N. Sanlier, Emine UTLU OZEN, S. Dilbaz, I. Kahyaoğlu, Y. Ustun","doi":"10.5472/marumj.1244652","DOIUrl":"https://doi.org/10.5472/marumj.1244652","url":null,"abstract":"Objective: The aim of our study was to evaluate sonographic endometrial thickness succeeding the estrogen-only stage and on the \u0000day when embryo transfer (ET) occurred in artificial frozen embryo transfer (FET) cycles to delve into the effect of endometrial \u0000compaction (EC) on clinical pregnancy rate (CPR). \u0000Patients and Methods: In the first group endometrial thickness diminished when ET occurred when compared to the end of the \u0000estrogen-only phase (n:37). Endometrial thickness increased/did not alter for the second group (n:70). \u0000Demographic characteristics were recorded and the following were studied: in vitro fertilization (IVF) treatment indications, hormone \u0000levels, total antral follicle count, duration of infertility, embryo quality, embryo-fundus distance, endometrial thickness at the end of \u0000estrogen-only phase and on ET day, luteal support, CPR. \u0000Results: No significant difference occurred in CPRs (n:107). ET, on day 5 was higher in the first group (p","PeriodicalId":43341,"journal":{"name":"Marmara Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42017915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Coplu, Ç. Kılınç, Aysegul Gozalan, B. Çalışır, C. Sonmez, Mustafa Muhammet Gul, Zeynep AYGUN AHLATCIOGLU
Objective: The reverse transcription–polymerase chain reaction test (RT-PCR) is the gold standard for the diagnosis of coronavirus disease 2019 (COVID-19), and antibody tests are useful as supplemental tools for diagnosis, for measuring the population’s immunity levels, and for checking infection in asymptomatic contacts. This study aimed to evaluate the reliability of five commercial antibody detection test kits. Materials and Methods: The reliability of the Colloidal Gold COVID-19 IgG/IgM Rapid Test Kit, Antibody Rapid Test Hotgen, Beijing Hotgen Biotech Co., Ltd., China), Abbott Chemiluminescent Microparticle Immunoassay (Illinois, USA), Roche Electrochemiluminescence Immunoassay (Roche Diagnostics, Switzerland), Siemens Chemiluminescence (Munich, Germany), and Euroimmun ELISA (Lübeck, Germany) for COVID-19 diagnosis was studied. The antibody-negative group included 50 sera from 2018, and the antibody-positive group included 98 patients with positive RT-PCR results from whom blood samples had been collected 3–9 weeks after hospital discharge. Statistical analysis was performed using SPSS version 23.0 (IBM Corporation, Armonk, NY, USA). The antibody tests’ validity and intra-assay reproducibility were examined, and the Cohen’s kappa coefficients were obtained. The disease prevalence was pegged at 10%. Results: The antibody tests’ sensitivity (69.12–72.46%) and positive predictive values (42.44–100.0%) were low, and their specificity (89.58–100%) and negative predictive values (96.31–97.03%) were high. Their accuracy rates varied from 87.54% to 97.25%, and their intra-assay coefficients of variation varied from 1% to 10%. Conclusion: The agreement between the results of the antibody detection test kits was higher when the kits were classified according to the targeted antigens. The time of blood sample collection, targeted antigens, and antibody types affected the results. Serological tests were found to be useful, and the commercial kits were found to be largely reliable, although, some parameters need to be improved.
{"title":"Reliability of antibody tests for COVID-19 diagnosis","authors":"N. Coplu, Ç. Kılınç, Aysegul Gozalan, B. Çalışır, C. Sonmez, Mustafa Muhammet Gul, Zeynep AYGUN AHLATCIOGLU","doi":"10.5472/marumj.1245068","DOIUrl":"https://doi.org/10.5472/marumj.1245068","url":null,"abstract":"Objective: The reverse transcription–polymerase chain reaction test (RT-PCR) is the gold standard for the diagnosis of coronavirus \u0000disease 2019 (COVID-19), and antibody tests are useful as supplemental tools for diagnosis, for measuring the population’s immunity \u0000levels, and for checking infection in asymptomatic contacts. This study aimed to evaluate the reliability of five commercial antibody \u0000detection test kits. \u0000Materials and Methods: The reliability of the Colloidal Gold COVID-19 IgG/IgM Rapid Test Kit, Antibody Rapid Test Hotgen, \u0000Beijing Hotgen Biotech Co., Ltd., China), Abbott Chemiluminescent Microparticle Immunoassay (Illinois, USA), Roche \u0000Electrochemiluminescence Immunoassay (Roche Diagnostics, Switzerland), Siemens Chemiluminescence (Munich, Germany), and \u0000Euroimmun ELISA (Lübeck, Germany) for COVID-19 diagnosis was studied. The antibody-negative group included 50 sera from \u00002018, and the antibody-positive group included 98 patients with positive RT-PCR results from whom blood samples had been collected \u00003–9 weeks after hospital discharge. Statistical analysis was performed using SPSS version 23.0 (IBM Corporation, Armonk, NY, USA). \u0000The antibody tests’ validity and intra-assay reproducibility were examined, and the Cohen’s kappa coefficients were obtained. The \u0000disease prevalence was pegged at 10%. \u0000Results: The antibody tests’ sensitivity (69.12–72.46%) and positive predictive values (42.44–100.0%) were low, and their specificity \u0000(89.58–100%) and negative predictive values (96.31–97.03%) were high. Their accuracy rates varied from 87.54% to 97.25%, and their \u0000intra-assay coefficients of variation varied from 1% to 10%. \u0000Conclusion: The agreement between the results of the antibody detection test kits was higher when the kits were classified according to \u0000the targeted antigens. The time of blood sample collection, targeted antigens, and antibody types affected the results. Serological tests \u0000were found to be useful, and the commercial kits were found to be largely reliable, although, some parameters need to be improved.","PeriodicalId":43341,"journal":{"name":"Marmara Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47691582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study investigated the relationship between parents’ emotional states and the anger and aggression levels of their children between the ages of 6-12 who were in social isolation during the coronavirus disease 2019 (COVID-19) pandemic in Turkey. Materials and Methods: The study adopted a cross-sectional and descriptive design. The sample consisted of 261 parents. Data were collected online during lockdowns between May and September 2020 due to the COVID-19 pandemic. Results: More than half of the participants reported that they were unhappy (53.2%), sad (63.2%), and worried about the future due to the COVID-19 pandemic (70.8%). Less than a quarter of the participants were angry (17.6%). Children living in districts had significantly higher verbal aggression scores than those living in city centers (p
{"title":"Anger and aggression in children aged 6-12 in lockdowns during the COVID-19 pandemic in Turkey","authors":"Hilal KURT SEZER, Nilay Bektas Akpinar, Merve Aşkin Ceran, Gozdenur Tanrikulu","doi":"10.5472/marumj.1244642","DOIUrl":"https://doi.org/10.5472/marumj.1244642","url":null,"abstract":"Objective: This study investigated the relationship between parents’ emotional states and the anger and aggression levels of their \u0000children between the ages of 6-12 who were in social isolation during the coronavirus disease 2019 (COVID-19) pandemic in Turkey. \u0000Materials and Methods: The study adopted a cross-sectional and descriptive design. The sample consisted of 261 parents. Data were \u0000collected online during lockdowns between May and September 2020 due to the COVID-19 pandemic. \u0000Results: More than half of the participants reported that they were unhappy (53.2%), sad (63.2%), and worried about the future due \u0000to the COVID-19 pandemic (70.8%). Less than a quarter of the participants were angry (17.6%). Children living in districts had \u0000significantly higher verbal aggression scores than those living in city centers (p","PeriodicalId":43341,"journal":{"name":"Marmara Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44785856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: We aimed to assess the possible relationship between plasma fibrinogen level and bone marrow fibrosis (BMF) grades in patients who had undergone bone marrow (BM) biopsy for any reason. Patients and Method: This retrospective cohort study included 106 participants aged 18 years and over who had undergone simultaneous BM biopsy and circulatory fibrinogen level measurement during 2020 and 2021 at our center. BMF grade was measured by the modified Bauermeister grading system (MBGS). Participants were divided into two groups according to MBGS as those without BMF and those with BMF. Results: Fifty-eight male were included in our study, and the median age of the patients was 63 (range: 19-97) years. Fibrinogen (p=0.004) and lactate dehydrogenase (LDH) (p=0.030) levels were significantly higher in the fibrosis group. Multiple regression revealed that high fibrinogen (≥359) and high LDH (≥238) were independently associated with a higher likelihood of fibrosis presence (adjusted for age and sex); however, diagnostic analyses revealed low accuracy. Conclusion: High plasma fibrinogen and LDH levels were found to be independently associated with the presence of BMF. However, it was also evident that neither of these parameters could be used for diagnostic purposes.
{"title":"Can plasma fibrinogen level predict bone marrow fibrosis?","authors":"Y. Ipek, Ayse N Kul","doi":"10.5472/marumj.1244611","DOIUrl":"https://doi.org/10.5472/marumj.1244611","url":null,"abstract":"Objective: We aimed to assess the possible relationship between plasma fibrinogen level and bone marrow fibrosis (BMF) grades in \u0000patients who had undergone bone marrow (BM) biopsy for any reason. \u0000Patients and Method: This retrospective cohort study included 106 participants aged 18 years and over who had undergone \u0000simultaneous BM biopsy and circulatory fibrinogen level measurement during 2020 and 2021 at our center. BMF grade was measured \u0000by the modified Bauermeister grading system (MBGS). Participants were divided into two groups according to MBGS as those without \u0000BMF and those with BMF. \u0000Results: Fifty-eight male were included in our study, and the median age of the patients was 63 (range: 19-97) years. Fibrinogen \u0000(p=0.004) and lactate dehydrogenase (LDH) (p=0.030) levels were significantly higher in the fibrosis group. Multiple regression \u0000revealed that high fibrinogen (≥359) and high LDH (≥238) were independently associated with a higher likelihood of fibrosis presence \u0000(adjusted for age and sex); however, diagnostic analyses revealed low accuracy. \u0000Conclusion: High plasma fibrinogen and LDH levels were found to be independently associated with the presence of BMF. However, \u0000it was also evident that neither of these parameters could be used for diagnostic purposes.","PeriodicalId":43341,"journal":{"name":"Marmara Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49162602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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