Accurate field monitoring of cadmium is hindered by electrode fouling and matrix interferences that plague conventional stripping voltammetry (SV). Here we introduce a differential-SV strategy that pairs two screen-printed working electrodes on the same chip (2WEs-SPCE) to decouple pre-concentration from signal read-out. The first electrode (WE1) accumulates Cd2+, while the second (WE2) records a background-free, non- pre-concentrated trace; subtracting the two currents suppresses drift and eliminates false positives. Both surfaces are nanostructured with a 1:1 graphene-oxide/carbon-nanohorn (GO/CNH) film that combines GO’s affinity for heavy metals with CNH’s high conductivity, boosting adsorption kinetics and signal stability. The sensor delivers dual linear ranges, 20–300 µg. L−1 (R2 = 0.994; 0.0415 µA. µg−1. L) and 300–1000 µg. L−1 (R2 = 0.986; 0.0241 µA. µg−1. L), with limit of detection and limit of quantification of 4.6 and 15.20 µg. L−1, respectively, appropriate for qualification of Cd2+ contamination in water sample. This differential 2WEs-SPCE platform marries low-cost printing with nanocarbon synergy, delivering interference-resistant, ppb-level cadmium sensing that is ready for on-site environmental and food-safety deployment.
{"title":"Stripping voltammetric analysis of cadmium on a dual working screen-printed carbon electrode modified with graphene-oxide/carbon-nanohorn composite","authors":"Watcharin Permwong , Jaroon Jakmunee , Somkid Pencharee","doi":"10.1016/j.talo.2026.100608","DOIUrl":"10.1016/j.talo.2026.100608","url":null,"abstract":"<div><div>Accurate field monitoring of cadmium is hindered by electrode fouling and matrix interferences that plague conventional stripping voltammetry (SV). Here we introduce a differential-SV strategy that pairs two screen-printed working electrodes on the same chip (2WEs-SPCE) to decouple pre-concentration from signal read-out. The first electrode (WE1) accumulates Cd<sup>2+</sup>, while the second (WE2) records a background-free, non- pre-concentrated trace; subtracting the two currents suppresses drift and eliminates false positives. Both surfaces are nanostructured with a 1:1 graphene-oxide/carbon-nanohorn (GO/CNH) film that combines GO’s affinity for heavy metals with CNH’s high conductivity, boosting adsorption kinetics and signal stability. The sensor delivers dual linear ranges, 20–300 µg. L<sup>−1</sup> (R<sup>2</sup> = 0.994; 0.0415 µA. µg<sup>−1</sup>. L) and 300–1000 µg. L<sup>−1</sup> (R<sup>2</sup> = 0.986; 0.0241 µA. µg<sup>−1</sup>. L), with limit of detection and limit of quantification of 4.6 and 15.20 µg. L<sup>−1</sup>, respectively, appropriate for qualification of Cd<sup>2+</sup> contamination in water sample. This differential 2WEs-SPCE platform marries low-cost printing with nanocarbon synergy, delivering interference-resistant, ppb-level cadmium sensing that is ready for on-site environmental and food-safety deployment.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100608"},"PeriodicalIF":3.7,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-05DOI: 10.1016/j.talo.2026.100611
Luca Gherardi , Francesca Viti , Salvatore Demartis , Marie McNulty , Giovanni Monteleone , Lorenzo Di Bari
Synthetic oligonucleotides often contain chemically modified backbones to prevent degradation and improve bioactivity. Phosphorothioate (PS) linkage in place of phosphodiester is the most used one. Substituting an oxygen for a sulfur atom introduces a stereogenic element on each PS, which leads to an exponentially large number of diastereomers, if the synthetic process is not stereoselective. Variable conditions during synthesis may lead to unpredictable variation in the manifold of diastereomers, with heavy fallouts in bioactivity. We developed an analytical protocol based on 31P-NMR followed by principal components analysis (PCA) to fingerprint each manifold. We assess the precision of this method by investigating repeatability and reproducibility. By working on two instruments at different magnetic fields, we also assess the problem of robustness and ultimately the possibility of exchanging data between laboratories.
{"title":"The use of 31P NMR and principal components analysis to determine sameness or differences between diastereomeric composition of phosphorothioate oligonucleotides","authors":"Luca Gherardi , Francesca Viti , Salvatore Demartis , Marie McNulty , Giovanni Monteleone , Lorenzo Di Bari","doi":"10.1016/j.talo.2026.100611","DOIUrl":"10.1016/j.talo.2026.100611","url":null,"abstract":"<div><div>Synthetic oligonucleotides often contain chemically modified backbones to prevent degradation and improve bioactivity. Phosphorothioate (PS) linkage in place of phosphodiester is the most used one. Substituting an oxygen for a sulfur atom introduces a stereogenic element on each PS, which leads to an exponentially large number of diastereomers, if the synthetic process is not stereoselective. Variable conditions during synthesis may lead to unpredictable variation in the manifold of diastereomers, with heavy fallouts in bioactivity. We developed an analytical protocol based on <sup>31</sup>P-NMR followed by principal components analysis (PCA) to fingerprint each manifold. We assess the precision of this method by investigating repeatability and reproducibility. By working on two instruments at different magnetic fields, we also assess the problem of robustness and ultimately the possibility of exchanging data between laboratories.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100611"},"PeriodicalIF":3.7,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-05DOI: 10.1016/j.talo.2026.100613
Xiaoyue Shan, Ji Shao, Ling Zhang, Yan Jin, Jiayi Qiu , Siwei Tan, Haipeng Ye, Luoxian Yang
In this study, a novel magnetic adsorbent (AC/β-CD/Fe3O4) was prepared for the magnetic solid phase extraction (MSPE) of hippuric acid (HA) from urine. The adsorbent combined the high surface area of activated carbon (AC), the host-guest recognition of β-cyclodextrin (β-CD), and the magnetic responsiveness of Fe3O4. Under the optimal conditions, the method demonstrated excellent performance for HA detection, with a linearity range of 0.0064-1.0 µg/mL, low limit of detection of 1.9 µg/L, and high precision (RSDs < 5%). Recoveries from spiked urine matrices ranged from 95% to 99%, confirming the method's accuracy and practicality for monitoring HA in real biological matrices.
{"title":"Preparation of activated carbon and β-cyclodextrin modified magnetic nanoparticles for extraction and determination of hippuric acid in urine samples","authors":"Xiaoyue Shan, Ji Shao, Ling Zhang, Yan Jin, Jiayi Qiu , Siwei Tan, Haipeng Ye, Luoxian Yang","doi":"10.1016/j.talo.2026.100613","DOIUrl":"10.1016/j.talo.2026.100613","url":null,"abstract":"<div><div>In this study, a novel magnetic adsorbent (AC/β-CD/Fe<sub>3</sub>O<sub>4</sub>) was prepared for the magnetic solid phase extraction (MSPE) of hippuric acid (HA) from urine. The adsorbent combined the high surface area of activated carbon (AC), the host-guest recognition of β-cyclodextrin (β-CD), and the magnetic responsiveness of Fe<sub>3</sub>O<sub>4</sub>. Under the optimal conditions, the method demonstrated excellent performance for HA detection, with a linearity range of 0.0064-1.0 µg/mL, low limit of detection of 1.9 µg/L, and high precision (RSDs < 5%). Recoveries from spiked urine matrices ranged from 95% to 99%, confirming the method's accuracy and practicality for monitoring HA in real biological matrices.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100613"},"PeriodicalIF":3.7,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-05DOI: 10.1016/j.talo.2026.100612
Sana Ahmed , Park Tusan
The persistent threat of foodborne pathogens, despite safety measures, underscores the urgent need for highly sensitive and rapid sensing technologies to prevent outbreaks and mitigate their devastating health and economic impacts. The evolution of sensing technologies, from enzyme-based biosensors to advanced immuno-based methods, highlights a continuous drive to improve sensitivity, speed, and feasibility in pathogen detection. Immuno-based biosensors have revolutionized pathogen detection by advancements in miniaturization, offering swift, sensitive, and cost-effective alternatives to traditional methods. Mainly, two categories of immuno-based biosensors have become largely demanding for POC; signal transduction-based, such as electrochemical, optical, and quartz crystal microbalance technologies, have significantly advanced pathogen detection by offering real-time, hyper-sensitive, and prompt monitoring capabilities, making them promising tools for field-deployable diagnostics. The second category, platform design-based immuno-based biosensors, such as lateral flow assays, microfluidic paper-based devices, and microchip-based devices, offer cost-effective, facile, and precise pathogen detection for use in food safety and outbreak prediction analysis. Nevertheless, numerous other biosensor types exist, but this review will focus on a few selective ones to enhance clarity and readability. The review shall summarize the state-of-the-art advancements in food pathogen sensing by immuno-based biosensors, their effectiveness, progress, and categories. Racing from qualification to quantification, the discussion will cover the challenges encountered and loopholes in the developed immuno-biosensing methodologies, including material-related issues (e.g., batch variability of nanomaterials, single-use paper substrates) and assay design (e.g., complex microfluidic architectures and multi-step protocols) that affect reproducibility, waste generation, and dependence on trained operators. It will reflect on a comparative study between the most recent emerging works based on good linear range, limit of detections (LODs), types of real samples utilized and short detection time for addressing their impact as POC devices. Unlike previous reviews that typically focus on isolated sensing mechanisms, this article provides a comparative analysis of both signal-transduction-based and platform-design-based immuno-biosensors, coupled with emphasis on recent advances relevant to point-of-care food pathogen detection.
{"title":"Next-generation immuno-biosensors: Transforming foodborne pathogen detection","authors":"Sana Ahmed , Park Tusan","doi":"10.1016/j.talo.2026.100612","DOIUrl":"10.1016/j.talo.2026.100612","url":null,"abstract":"<div><div>The persistent threat of foodborne pathogens, despite safety measures, underscores the urgent need for highly sensitive and rapid sensing technologies to prevent outbreaks and mitigate their devastating health and economic impacts. The evolution of sensing technologies, from enzyme-based biosensors to advanced immuno-based methods, highlights a continuous drive to improve sensitivity, speed, and feasibility in pathogen detection. Immuno-based biosensors have revolutionized pathogen detection by advancements in miniaturization, offering swift, sensitive, and cost-effective alternatives to traditional methods. Mainly, two categories of immuno-based biosensors have become largely demanding for POC; signal transduction-based, such as electrochemical, optical, and quartz crystal microbalance technologies, have significantly advanced pathogen detection by offering real-time, hyper-sensitive, and prompt monitoring capabilities, making them promising tools for field-deployable diagnostics. The second category, platform design-based immuno-based biosensors, such as lateral flow assays, microfluidic paper-based devices, and microchip-based devices, offer cost-effective, facile, and precise pathogen detection for use in food safety and outbreak prediction analysis. Nevertheless, numerous other biosensor types exist, but this review will focus on a few selective ones to enhance clarity and readability. The review shall summarize the state-of-the-art advancements in food pathogen sensing by immuno-based biosensors, their effectiveness, progress, and categories. Racing from qualification to quantification, the discussion will cover the challenges encountered and loopholes in the developed immuno-biosensing methodologies, including material-related issues (e.g., batch variability of nanomaterials, single-use paper substrates) and assay design (e.g., complex microfluidic architectures and multi-step protocols) that affect reproducibility, waste generation, and dependence on trained operators. It will reflect on a comparative study between the most recent emerging works based on good linear range, limit of detections (LODs), types of real samples utilized and short detection time for addressing their impact as POC devices. Unlike previous reviews that typically focus on isolated sensing mechanisms, this article provides a comparative analysis of both signal-transduction-based and platform-design-based immuno-biosensors, coupled with emphasis on recent advances relevant to point-of-care food pathogen detection.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100612"},"PeriodicalIF":3.7,"publicationDate":"2026-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-24DOI: 10.1016/j.talo.2025.100606
Xin Li , Kaigang An , Shuang Zhang , Jing He , Huifeng Liu , Hongdeng Qiu , Yiqun Wan , Jia Chen
Rapid detection of antibiotic residues in food is essential for public health. This study aims to develop a highly sensitive colorimetric sensing platform based on a novel nanozyme. To this end, we used a green, tunable, and safe choline chloride/urea-based deep eutectic solvent (DES) to synthesize a metal organic framework, Cu-BTC (BTC: 1,3,5-benzenetricarboxylate), with many highly dispersed copper active sites. As a result, the prepared Cu-BTC showed strong peroxidase-like activity. In the presence of hydrogen peroxide (H2O2), it efficiently catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue product TMBox. Importantly, we found that tetracycline (TC) specifically and significantly inhibits this color reaction. The inhibition occurred because TC can directly reduce the blue TMBox, suppressing color development. Based on this effect, we developed a new colorimetric method for sensitive TC analysis. The method had a wide linear range (0.025–25 μM) and a very low detection limit (0.0024 μM). Moreover, it was successfully applied to accurately detect TC in complex food matrices such as milk and honey. Thus, this work not only provides a green route to synthesize high-performance nanozymes, but also establishes a simple, fast, and sensitive colorimetric platform for on-site detection of antibiotic residues in food.
{"title":"Green nanozyme, rapid sensing: A DES-synthesized Cu-BTC platform for rapid tetracycline detection in food","authors":"Xin Li , Kaigang An , Shuang Zhang , Jing He , Huifeng Liu , Hongdeng Qiu , Yiqun Wan , Jia Chen","doi":"10.1016/j.talo.2025.100606","DOIUrl":"10.1016/j.talo.2025.100606","url":null,"abstract":"<div><div>Rapid detection of antibiotic residues in food is essential for public health. This study aims to develop a highly sensitive colorimetric sensing platform based on a novel nanozyme. To this end, we used a green, tunable, and safe choline chloride/urea-based deep eutectic solvent (DES) to synthesize a metal organic framework, Cu-BTC (BTC: 1,3,5-benzenetricarboxylate), with many highly dispersed copper active sites. As a result, the prepared Cu-BTC showed strong peroxidase-like activity. In the presence of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), it efficiently catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue product TMBox. Importantly, we found that tetracycline (TC) specifically and significantly inhibits this color reaction. The inhibition occurred because TC can directly reduce the blue TMBox, suppressing color development. Based on this effect, we developed a new colorimetric method for sensitive TC analysis. The method had a wide linear range (0.025–25 μM) and a very low detection limit (0.0024 μM). Moreover, it was successfully applied to accurately detect TC in complex food matrices such as milk and honey. Thus, this work not only provides a green route to synthesize high-performance nanozymes, but also establishes a simple, fast, and sensitive colorimetric platform for on-site detection of antibiotic residues in food.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100606"},"PeriodicalIF":3.7,"publicationDate":"2025-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-11DOI: 10.1016/j.talo.2025.100605
Yahui Song , Yifei Wang , Honghui Tang
Objective
This study aimed to establish a detection method for serum interleukin 12p70 (IL-12p70) using digested Fab' antibody fragments and acridine esterification chemiluminescence immunoquantitative analysis.
Methods
The double-antibody sandwich method was used with the complete IL-12p70 antibody labeled with acridine ester and an enzymatically digested antibody fragment of p40 labeled with biotin. A sandwich complex then formed with the analyte IL-12p70, in which biotin was captured by magnetic solid-phase particles coated with streptavidin, which glowed under alkaline hydrogen peroxide excitation. The luminescent signal was quantitatively analyzed. The stability of the labeled antibodies and the performance of the new quantitative method was evaluated. The clinical analysis results were compared with flow cytometry fluorescence to verify the clinical performance.
Results
Acridine ester sulfonamide and liposoluble biotin showed good stability as antibody labels. The limit of blank was 0.1 pg/mL and the limit of detection was 0.5 pg/mL, determined according to CLSI EP17-A2 with n = 60 replicates per batch with two independent batches. The linearity was 0.5–5000 pg/mL, and the precision was < 5.5 %. The results of the new method and flow cytometry fluorescence were highly correlated (P < 0.05). Interfering substances, including triglyceride, hemoglobin, bilirubin, rheumatoid factor, heterophilic human anti-mouse antibodies, and the structural analogs IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, and IL-23 had no significant effect on the results.
Conclusion
A method for detecting IL-12p70 in serum was established based on immunoquantitative analysis using Fab' antibody fragments, biotin and streptavidin amplification effects, and acridine ester chemiluminescence. The method exhibited high sensitivity, strong specificity, and a wide detection range, indicating its strong clinical application prospects.
{"title":"Establishment and preliminary clinical evaluation of a high-sensitivity chemiluminescence immunoassay for serum IL-12p70 using fab' antibody fragments and acridinium ester","authors":"Yahui Song , Yifei Wang , Honghui Tang","doi":"10.1016/j.talo.2025.100605","DOIUrl":"10.1016/j.talo.2025.100605","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to establish a detection method for serum interleukin 12p70 (IL-12p70) using digested Fab' antibody fragments and acridine esterification chemiluminescence immunoquantitative analysis.</div></div><div><h3>Methods</h3><div>The double-antibody sandwich method was used with the complete IL-12p70 antibody labeled with acridine ester and an enzymatically digested antibody fragment of p40 labeled with biotin. A sandwich complex then formed with the analyte IL-12p70, in which biotin was captured by magnetic solid-phase particles coated with streptavidin, which glowed under alkaline hydrogen peroxide excitation. The luminescent signal was quantitatively analyzed. The stability of the labeled antibodies and the performance of the new quantitative method was evaluated. The clinical analysis results were compared with flow cytometry fluorescence to verify the clinical performance.</div></div><div><h3>Results</h3><div>Acridine ester sulfonamide and liposoluble biotin showed good stability as antibody labels. The limit of blank was 0.1 pg/mL and the limit of detection was 0.5 pg/mL, determined according to CLSI EP17-A2 with <em>n</em> = 60 replicates per batch with two independent batches. The linearity was 0.5–5000 pg/mL, and the precision was < 5.5 %. The results of the new method and flow cytometry fluorescence were highly correlated (<em>P</em> < 0.05). Interfering substances, including triglyceride, hemoglobin, bilirubin, rheumatoid factor, heterophilic human anti-mouse antibodies, and the structural analogs IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, and IL-23 had no significant effect on the results.</div></div><div><h3>Conclusion</h3><div>A method for detecting IL-12p70 in serum was established based on immunoquantitative analysis using Fab' antibody fragments, biotin and streptavidin amplification effects, and acridine ester chemiluminescence. The method exhibited high sensitivity, strong specificity, and a wide detection range, indicating its strong clinical application prospects.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100605"},"PeriodicalIF":3.7,"publicationDate":"2025-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marine and freshwater biotoxins threaten public health and seafood safety due to their tendency to accumulate in fish and shellfish. This systematic literature review summarises findings from 51 peer-reviewed studies published between 2004 and 2025 that focus on nanoparticle-enhanced lateral flow immunoassays (LFIAs) for toxin detection. Gold nanoparticles (AuNPs) were by far the most used label, employed in ∼75 % of studies, which consistently delivered the lowest limits of detection (LODs) as low as 0.03 ng/ml for okadaic acid detection. Despite increasing concerns over the co-occurrence of multiple toxins, ∼88 % of the studies were based on single-analyte detection, only ∼12 % employing multiplexed approaches. Statistical analyses of the studies, namely Kruskal–Wallis, Dunn’s post hoc, correlation, and regression analysis, revealed meaningful trends. LODs have significantly improved over time, a marked reduction being observed after 2016. Kruskal–Wallis and Dunn’s post hoc tests showed significant differences across variables such as nanoparticle size, assay duration, and toxin recovery. Spearman correlation results identified a moderately significant negative correlation of LOD with publication year (ρ = –0.458, p < 0.001), and a weaker significant correlation of LOD with assay time (ρ = –0.301, p = 0.018), while linear regression analysis identified publication year (R² = 0.2441, p < 0.0001) and nanoparticle size (R² = 0.1072, p = 0.0264) as significant predictors of assay sensitivity. Overall, this review underscores the central role of nanoparticle engineering, simplified assay workflows, and optimized design strategies in improving LFIA performance. The findings provide a valuable roadmap for advancing the development of portable, sensitive, and scalable biosensing platforms for marine toxin monitoring and seafood safety surveillance.
海洋和淡水生物毒素由于易于在鱼类和贝类中积累，威胁着公众健康和海产品安全。本系统文献综述总结了2004年至2025年间发表的51项同行评审研究的结果，这些研究的重点是用于毒素检测的纳米颗粒增强侧流免疫测定法（LFIAs）。金纳米颗粒（AuNPs）是迄今为止使用最多的标签，在约75%的研究中使用，其始终提供最低检测限（lod）低至0.03 ng/ml的冈田酸检测。尽管对多种毒素共存的担忧日益增加，但约88%的研究是基于单一分析物检测，只有约12%采用多重方法。这些研究的统计分析，即Kruskal-Wallis、Dunn的事后分析、相关分析和回归分析，揭示了有意义的趋势。随着时间的推移，lod显着改善，2016年之后观察到明显减少。Kruskal-Wallis和Dunn的事后测试显示，在纳米颗粒大小、测定时间和毒素回收率等变量之间存在显著差异。Spearman相关结果显示，LOD与发表年份呈中显著负相关（ρ = -0.458, p < 0.001），与检测时间呈弱显著相关（ρ = -0.301, p = 0.018），而线性回归分析发现，发表年份（R²= 0.2441,p < 0.0001）和纳米颗粒大小（R²= 0.1072,p = 0.0264）是检测灵敏度的显著预测因子。总之，这篇综述强调了纳米颗粒工程、简化分析工作流程和优化设计策略在提高LFIA性能方面的核心作用。研究结果为推进便携式、灵敏和可扩展的海洋毒素监测和海产品安全监测生物传感平台的发展提供了有价值的路线图。
{"title":"Nanoparticle-based lateral flow immunoassays for detecting marine biotoxins in seafood: a systematic review of trends in development over two decades","authors":"Perveen Akhtar , Gayathree Thenuwara , Hugh J. Byrne , Furong Tian , Bilal Javed","doi":"10.1016/j.talo.2025.100604","DOIUrl":"10.1016/j.talo.2025.100604","url":null,"abstract":"<div><div>Marine and freshwater biotoxins threaten public health and seafood safety due to their tendency to accumulate in fish and shellfish. This systematic literature review summarises findings from 51 peer-reviewed studies published between 2004 and 2025 that focus on nanoparticle-enhanced lateral flow immunoassays (LFIAs) for toxin detection. Gold nanoparticles (AuNPs) were by far the most used label, employed in ∼75 % of studies, which consistently delivered the lowest limits of detection (LODs) as low as 0.03 ng/ml for okadaic acid detection. Despite increasing concerns over the co-occurrence of multiple toxins, ∼88 % of the studies were based on single-analyte detection, only ∼12 % employing multiplexed approaches. Statistical analyses of the studies, namely Kruskal–Wallis, Dunn’s post hoc, correlation, and regression analysis, revealed meaningful trends. LODs have significantly improved over time, a marked reduction being observed after 2016. Kruskal–Wallis and Dunn’s post hoc tests showed significant differences across variables such as nanoparticle size, assay duration, and toxin recovery. Spearman correlation results identified a moderately significant negative correlation of LOD with publication year (ρ = –0.458, <em>p</em> < 0.001), and a weaker significant correlation of LOD with assay time (ρ = –0.301, <em>p</em> = 0.018), while linear regression analysis identified publication year (R² = 0.2441, <em>p</em> < 0.0001) and nanoparticle size (R² = 0.1072, <em>p</em> = 0.0264) as significant predictors of assay sensitivity. Overall, this review underscores the central role of nanoparticle engineering, simplified assay workflows, and optimized design strategies in improving LFIA performance. The findings provide a valuable roadmap for advancing the development of portable, sensitive, and scalable biosensing platforms for marine toxin monitoring and seafood safety surveillance.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100604"},"PeriodicalIF":3.7,"publicationDate":"2025-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-05DOI: 10.1016/j.talo.2025.100602
Suvarna Yenduri, Ramya P, Naga Prashant K
Natural antioxidants, primarily produced from plants, have significant roles in avoiding oxidative stress related issues, enhancing shelf life of food, and improving human health. Because of the existence of wide range of antioxidants like flavonoids, phenolic acids, tannins, carotenoids, vitamins, it is difficult for characterization and evaluation of antioxidants. Conventional antioxidant assays are commonly employed till today; however they provide limited structural and biological insights. These constraints can be overcome by the application of advanced analytical techniques like chromatography, spectroscopy etc. which provide greater sensitivity and more accurate structural characterization. Recent research has highlighted the use of these approaches for qualitative and quantitative analysis, also for evaluating antioxidant activity in various matrices such as plant materials, food, nutraceuticals etc. The present study summarizes recent developments, comparison of various analytical methods and also highlighted the advantages and limitations of those methods. Emerging analytical techniques that combine green extraction methods for sample preparation, hyphenated analytical technique for analysis and chemometrics for data interpretation hold promise for reliable, rapid and sustainable natural antioxidant analysis.
{"title":"Recent advances in analytical methods and instrumentation for natural antioxidants analysis","authors":"Suvarna Yenduri, Ramya P, Naga Prashant K","doi":"10.1016/j.talo.2025.100602","DOIUrl":"10.1016/j.talo.2025.100602","url":null,"abstract":"<div><div>Natural antioxidants, primarily produced from plants, have significant roles in avoiding oxidative stress related issues, enhancing shelf life of food, and improving human health. Because of the existence of wide range of antioxidants like flavonoids, phenolic acids, tannins, carotenoids, vitamins, it is difficult for characterization and evaluation of antioxidants. Conventional antioxidant assays are commonly employed till today; however they provide limited structural and biological insights. These constraints can be overcome by the application of advanced analytical techniques like chromatography, spectroscopy etc. which provide greater sensitivity and more accurate structural characterization. Recent research has highlighted the use of these approaches for qualitative and quantitative analysis, also for evaluating antioxidant activity in various matrices such as plant materials, food, nutraceuticals etc. The present study summarizes recent developments, comparison of various analytical methods and also highlighted the advantages and limitations of those methods. Emerging analytical techniques that combine green extraction methods for sample preparation, hyphenated analytical technique for analysis and chemometrics for data interpretation hold promise for reliable, rapid and sustainable natural antioxidant analysis.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100602"},"PeriodicalIF":3.7,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-05DOI: 10.1016/j.talo.2025.100603
Francesca R. Pennecchi , Tamar Gadrich , Ilya Kuselman , D. Brynn Hibbert , Angelique Botha , Anastasia A. Semenova
Decision-making risks caused by human errors in performing a chemical analysis are assessed using laboratory expert judgments (responses) on a specified ordinal scale. In the present paper, a new approach to assessment of risk is described based on implementation of the recently developed two-way ordinal analysis of variation – ORDANOVA. This approach calculates the number of expert responses related to the same category for each ordinal characteristic and then analyzes their relative frequencies as fractions of the total number of responses (of all categories) obtained for this characteristic. It does not violate the properties of ordinal data and allows for the correct interpretation of expert responses. Previously published expert responses on the risks in pH measurements of groundwater, in gas chromatography–mass spectrometry multi-residue pesticide analysis of fruits and vegetables, and in inductively coupled plasma–mass spectrometry analysis of geological samples, are analysed as examples. The datasets prepared for ORDANOVA calculations with the freely available software tool are provided in supplementary materials to the paper. The reduction of risk by different components of the laboratory quality system (QS) are estimated under several error scenarios. New multinomial scores characterizing risk reduction by the laboratory QS as a whole are proposed.
{"title":"Human errors in an analytical chemistry laboratory - Implementation of ordinal analysis of variation for risk assessment","authors":"Francesca R. Pennecchi , Tamar Gadrich , Ilya Kuselman , D. Brynn Hibbert , Angelique Botha , Anastasia A. Semenova","doi":"10.1016/j.talo.2025.100603","DOIUrl":"10.1016/j.talo.2025.100603","url":null,"abstract":"<div><div>Decision-making risks caused by human errors in performing a chemical analysis are assessed using laboratory expert judgments (responses) on a specified ordinal scale. In the present paper, a new approach to assessment of risk is described based on implementation of the recently developed two-way ordinal analysis of variation – ORDANOVA. This approach calculates the number of expert responses related to the same category for each ordinal characteristic and then analyzes their relative frequencies as fractions of the total number of responses (of all categories) obtained for this characteristic. It does not violate the properties of ordinal data and allows for the correct interpretation of expert responses. Previously published expert responses on the risks in pH measurements of groundwater, in gas chromatography–mass spectrometry multi-residue pesticide analysis of fruits and vegetables, and in inductively coupled plasma–mass spectrometry analysis of geological samples, are analysed as examples. The datasets prepared for ORDANOVA calculations with the freely available software tool are provided in supplementary materials to the paper. The reduction of risk by different components of the laboratory quality system (QS) are estimated under several error scenarios. New multinomial scores characterizing risk reduction by the laboratory QS as a whole are proposed.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100603"},"PeriodicalIF":3.7,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We report a metal-free perylene-based polyimide framework (PDI-PB) as an efficient electrocatalyst for oxygen reduction reaction (ORR)-enhanced cathodic electrochemiluminescence (ECL). PDI-PB enhances reactive oxygen species (ROS) generation via oxygen reduction that significantly amplify the luminol–dissolved oxygen ECL signal. The resulting biosensor enables ultrasensitive detection of dopamine (DA) over a wide linear range (5 × 10–5 to 100 μM) with a record-low detection limit of 9.76 pM (S/N = 3). The sensor exhibits excellent selectivity, stability, and reproducibility, and was successfully applied to detect DA in human sweat, serum, and urine, yielding recoveries of 97.0–101.1 %. Density functional theory (DFT) calculations reveal that PDI-PB’s superior electronic structure facilitates efficient charge transfer and oxygen activation. This work presents a new strategy for metal-free organic electrocatalyst design, combining mechanistic insight with high-performance ECL biosensing for non-invasive health monitoring and clinical diagnostics.
{"title":"Metal-free perylene-based polyimide frameworks for oxygen reduction-enhanced electrochemiluminescence biosensing","authors":"Qiao Chen , Xiaoran Zhang , Xinyue Zhou , Peng Huang , Daming Feng , Xu Xu , Chunhua Ge","doi":"10.1016/j.talo.2025.100600","DOIUrl":"10.1016/j.talo.2025.100600","url":null,"abstract":"<div><div>We report a metal-free perylene-based polyimide framework (PDI-PB) as an efficient electrocatalyst for oxygen reduction reaction (ORR)-enhanced cathodic electrochemiluminescence (ECL). PDI-PB enhances reactive oxygen species (ROS) generation via oxygen reduction that significantly amplify the luminol–dissolved oxygen ECL signal. The resulting biosensor enables ultrasensitive detection of dopamine (DA) over a wide linear range (5 × 10<sup>–5</sup> to 100 μM) with a record-low detection limit of 9.76 pM (S/<em>N</em> = 3). The sensor exhibits excellent selectivity, stability, and reproducibility, and was successfully applied to detect DA in human sweat, serum, and urine, yielding recoveries of 97.0–101.1 %. Density functional theory (DFT) calculations reveal that PDI-PB’s superior electronic structure facilitates efficient charge transfer and oxygen activation. This work presents a new strategy for metal-free organic electrocatalyst design, combining mechanistic insight with high-performance ECL biosensing for non-invasive health monitoring and clinical diagnostics.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100600"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号