Talanta Open最新文献_第2页

Electrochemical immunosensor based on screen-printed electrodes modified with gold nanoparticles for highly sensitive detection of TGF-α 基于金纳米粒子修饰的丝网印刷电极的电化学免疫传感器用于TGF-α的高灵敏度检测

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-11-05 DOI: 10.1016/j.talo.2025.100590

Smriti Gaba, Utkarsh Jain

Transforming Growth Factor Alpha (TGF-α) is a critical biomarker associated with various malignancies and pathological conditions, including cancer and other diseases. For efficient diagnosis and therapeutic monitoring, TGF-α in clinical serum must be detected accurately. In this study, we present a highly sensitive and selective electrochemical immunosensor for the detection of TGF-α, leveraging the principles of immunorecognition and advanced signal transduction. The immunosensor was constructed by immobilizing monoclonal anti-TGF-α antibodies via EDCNHS chemistry onto a screen-printed electrode surface modified with gold nanoparticles to enhance electron transfer and provide a high surface area for antibody binding. Gold nanomaterials were characterized by UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS), and Transmission electron microscopy (TEM) to confirm their morphology and size. Cyclic voltammetry (CV) and X-ray photoelectron spectroscopy (XPS) were employed to characterize the electrode modifications, ensuring stability and functional integrity. The developed sensor demonstrated excellent analytical performance, with a detection limit of 0.35 pg mL⁻¹, a linear range of 1–1000 pg mL⁻¹, and a high sensitivity of 0.051 mA. The immunosensor demonstrated high specificity, with negligible interference from other serum proteins, and robust repeatability. The sensor retained about 98% of its original response after storage at 4°C for 1 week, demonstrating acceptable long-term stability of the functionalized platform. This study underscores the potential of electrochemical immunosensors as a promising platform for the point-of-care diagnostics of TGF-α, enabling timely and accurate disease management. Future advancements may integrate multiplex detection capabilities and real serum analysis for broader clinical applicability and real-world applications.

转化生长因子α (TGF-α)是一种重要的生物标志物，与各种恶性肿瘤和病理状况相关，包括癌症和其他疾病。为了有效的诊断和治疗监测，必须准确检测临床血清TGF-α。在本研究中，我们利用免疫识别和高级信号转导的原理，提出了一种高灵敏度和选择性的检测TGF-α的电化学免疫传感器。该免疫传感器通过EDCNHS化学将单克隆抗tgf -α抗体固定在用金纳米颗粒修饰的丝网印刷电极表面上，以增强电子传递并为抗体结合提供高表面积。利用紫外可见光谱（UV-Vis）、傅里叶变换红外光谱（FTIR）、动态光散射（DLS）和透射电子显微镜（TEM）对金纳米材料进行了表征，以确定其形貌和尺寸。利用循环伏安法（CV）和x射线光电子能谱法（XPS）对电极修饰进行了表征，确保了电极的稳定性和功能完整性。该传感器具有良好的分析性能，检测限为0.35 pg mL - 1，线性范围为1-1000 pg mL - 1，灵敏度为0.051 mA。该免疫传感器具有高特异性，可忽略其他血清蛋白的干扰，并且具有良好的重复性。在4°C下储存1周后，传感器保留了约98%的原始响应，表明功能化平台具有可接受的长期稳定性。这项研究强调了电化学免疫传感器作为TGF-α即时诊断平台的潜力，从而实现及时准确的疾病管理。未来的进展可能会整合多重检测能力和真实血清分析，以获得更广泛的临床适用性和现实应用。

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引用次数: 0

Simultaneous determination of 11 haloacetic acids and oxyhalides by UPLC-HRMS in Human Serum, Breast Milk, and Urine UPLC-HRMS同时测定人血清、母乳和尿液中的11种卤乙酸和氧卤化物

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-11-04 DOI: 10.1016/j.talo.2025.100589

Zelin Wang, Xiaoyu Hu, Qiaozhen Guo, Mengdie Fan, Yang Xing, Sai Fan, Yunjia Yang, Jing Zhang, Bing Shao

Disinfection byproducts (DBPs), particularly haloacetic acids (HAAs) and oxyhalides, are of global concern due to their carcinogenicity and occurrence beyond drinking water. Biomonitoring data in human biological matrices remain scarce, especially for emerging iodinated DBPs. This study presents a validated analytical method for simultaneously quantifying 11 HAAs and oxyhalides in serum, urine, and breast milk. This method combines a tailored solid-phase clean-up with ultra-performance liquid chromatography–high-resolution mass spectrometry (UPLCHRMS) and achieves excellent recoveries of 77–117 %, low method detection limits of 0.01–0.20 μg l^-1, and acceptable matrix effects across all matrices. Applied to 122 biological samples collected in Beijing, this approach revealed several unprecedented findings: (i) mono-iodoacetic acid (MIAA), a highly toxic iodinated DBP, was consistently detected in all three matrices, marking the first such report in human biomonitoring; (ii) chlorate was detected in human urine for the first time, with a mean concentration of 117 μg l^-1, indicating overlooked exposure pathways; and (iii) significant correlations between HAAs and oxyhalides in biological samples suggest shared exposure sources or metabolic processes not previously documented. Serum exhibited the highest HAA concentrations, suggesting its high body burden. Chlorate and perchlorate were frequently detected at high concentrations in urine and breast milk, highlighting possible risks to vulnerable populations. This study addresses critical gaps in methodology and exposure data, providing a robust tool for DBP biomonitoring and key evidence for cumulative risk assessment beyond drinking water.

消毒副产物（DBPs），特别是卤代乙酸（HAAs）和氧卤化物，由于其致癌性和在饮用水之外的存在而受到全球关注。人类生物基质的生物监测数据仍然很少，特别是对于新出现的碘化dbp。本研究提出了一种有效的分析方法，用于同时定量血清、尿液和母乳中的11 HAAs和氧卤化物。该方法结合了定制的固相净化和超高效液相色谱-高分辨率质谱（UPLCHRMS），回收率为77 - 117%，方法检出限为0.01-0.20 μg -1，所有基质的基质效应均可接受。将该方法应用于北京采集的122个生物样本，揭示了几个前所未有的发现：(i)在所有三种基质中均检测到高毒性碘化DBP -单碘乙酸（MIAA），这是人类生物监测中首次报道；（ii）首次在人尿中检出氯酸盐，平均浓度为117 μg l-1，暴露途径被忽视；（iii）生物样品中HAAs和氧化卤化物之间的显著相关性表明，以前没有文献记载的共同暴露源或代谢过程。血清中HAA浓度最高，表明其机体负荷高。尿液和母乳中经常检测到高浓度氯酸盐和高氯酸盐，这突出了弱势群体可能面临的风险。本研究解决了方法和暴露数据方面的关键空白，为DBP生物监测提供了强有力的工具，并为饮用水以外的累积风险评估提供了关键证据。

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引用次数: 0

From carob to carbon dots: Sustainable synthesis of fluorescent nanosensor for sensitive secnidazole determination in pharmaceutical, biological, and environmental samples 从角豆到碳点：可持续合成的荧光纳米传感器用于制药、生物和环境样品中塞克尼唑的灵敏测定

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-11-04 DOI: 10.1016/j.talo.2025.100588

Raneem Hesham , Heba Abd El-Aziz , Rania El-Shaheny

A fast, one pot, eco-friendly, and affordable fluorescent nanoprobe for the antimicrobial drug secnidazole was created. Highly luminescent N-doped carbon nanoparticles (N-CNPs) were produced for the first time from carob fruit extract as a readily available precursor via microwave-assisted synthesis in about six minutes. The developed N-CNPs exhibited good stability and water solubility. EDX, TEM, XPS, XRD, Zeta potential analysis, UV-visible spectroscopy, Fourier transform infrared, and fluorescence spectroscopy were used to characterize the N-CNPs comprehensively. The furnished N-CNPs demonstrated outstanding capabilities as a fluorescence nanosensor for secnidazole detection at λ_ex/λ_em of 330/425 nm within a concentration range from 10.0 to 200.0 μM and a limit of detection of 2.8 μM. The fluorescence of the proposed N-CNPs was successfully quenched by secnidazole via the inner filter effect. The nanoprobe exhibits excellent secnidazole selectivity even when a variety of potentially interfering drugs, excipients, or ions are present. This approach was used to analyze secnidazole in pharmaceutical dosage forms with a recovery percentage of 99.52 ± 1.37 % for Fladazole tablets® and 101.6 ± 1.92 % for Cipazole tablets® and in tap and irrigation water with recovery percentages of 101.1 ± 3.2 % and 99.23 ± 1.11 %, respectively. Furthermore, the N-CNPs have been employed to monitor the urinary elimination of SNZ with a limit of detection of 6.95 μM. The use of an economical and sustainable plant as a substrate through microwave-assisted synthesis shows excellent greenness and blueness characteristics of the suggested probe as assessed by AGREE, ComplexGAPI, and BAGI, respectively.

研制了一种快速、一锅、环保、价格合理的抗微生物药物塞克硝唑荧光纳米探针。本文首次以角豆果提取物为原料，通过微波辅助合成，在6分钟内制备了高发光n掺杂碳纳米颗粒（N-CNPs）。制备的N-CNPs具有良好的稳定性和水溶性。利用EDX、TEM、XPS、XRD、Zeta电位分析、紫外-可见光谱、傅里叶变换红外光谱和荧光光谱对N-CNPs进行了全面表征。所制备的N-CNPs作为一种荧光纳米传感器，在λex/λem范围为330/425 nm，检测浓度为10.0 ~ 200.0 μM，检测限为2.8 μM。所提出的N-CNPs的荧光被塞克硝唑通过内过滤效应成功猝灭。即使存在多种潜在干扰药物、赋形剂或离子，纳米探针也表现出优异的塞硝唑选择性。采用该方法对药用剂型中的塞克硝唑进行分析，弗拉唑片®的回收率为99.52±1.37%，西帕唑片®的回收率为101.6±1.92%，自来水和灌溉水的回收率分别为101.1±3.2%和99.23±1.11%。此外，N-CNPs还被用于监测尿中SNZ的消除，检测限为6.95 μM。利用经济和可持续的植物作为底物，通过微波辅助合成表明，所建议的探针具有优异的绿色和蓝色特性，分别通过AGREE， ComplexGAPI和BAGI进行了评估。

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引用次数: 0

Accuracy assessment of a micro-Raman spectroscopy method for small microplastic particles in infant milk formula 微拉曼光谱法测定婴儿配方奶粉中塑料微粒的准确性评价

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-31 DOI: 10.1016/j.talo.2025.100586

Mara Putzu , Marta Barbaresi , Marta Fadda , Alessio Sacco , Maurizio Piergiovanni , Matteo Masino , Federica Bianchi , Korinna Altmann , Nizar Benismail , Laureen Coïc , Ivana Fenoglio , Monica Mattarozzi , Andrea Mario Rossi , Maria Careri , Andrea Mario Giovannozzi

The presence of microplastics (MPs) in the food chain is increasingly documented, raising concerns over potential risks to human health. Despite growing efforts, standardized methods for MPs detection in food matrices remain limited. This study presents an interlaboratory comparison (ILC) aimed at assessing the accuracy and comparability of an analytical approach for the identification and quantification of small MPs (5–100 µm) in infant milk powder using µ-Raman spectroscopy and a representative polyethylene terephthalate (PET) reference material (RM). The RM, formulated as water-soluble tablets, was designed to replicate the morphology, size distribution, and polymer composition of environmentally relevant MPs, and was previously assessed for homogeneity and stability for mass fraction and particle numbers.

The approach was assessed using two PET RM batches with different MPs particle numbers (high load batch: 1759 ± 141 MPs; low load batch: 160 ± 22 MPs), subjected to an enzymatic–chemical digestion, followed by µ-Raman analysis performed independently in two laboratories with different instruments and operators. Results are reported as absolute particle counts per analyzed sample and demonstrated excellent recovery across all size classes, including the smallest particles (down to 5 µm), with recovery rates ranging from 82 % to 88 %, in good agreement with the RM reference values.

The analytical approach proved to be robust, reproducible, and suitable for low-level MPs quantification in complex food matrices, supporting ongoing efforts toward method harmonization and standardization for reliable MPs monitoring in the food sector.

微塑料（MPs）在食物链中的存在被越来越多地记录下来，引发了人们对人类健康潜在风险的担忧。尽管越来越多的努力，在食品基质中检测MPs的标准化方法仍然有限。本研究提出了一种实验室间比较（ILC），旨在评估使用微拉曼光谱和具有代表性的聚对苯二甲酸乙二醇酯（PET）标准物质（RM）鉴定和定量婴儿奶粉中小MPs （5-100 μ m）的分析方法的准确性和可比性。制剂为水溶性片剂，旨在复制环境相关MPs的形态、大小分布和聚合物组成，并预先评估了质量分数和颗粒数的均匀性和稳定性。采用两个具有不同MPs颗粒数的PET RM批次（高负载批次：1759±141 MPs；低负载批次：160±22 MPs）进行酶化学消化，然后在两个实验室使用不同的仪器和操作人员独立进行µ拉曼分析。结果报告为每个分析样品的绝对颗粒计数，并且在所有尺寸类别中都表现出出色的回收率，包括最小的颗粒（低至5 μ m），回收率从82%到88%，与RM参考值很好地一致。该分析方法被证明是稳健的、可重复的，并且适用于复杂食品基质中的低水平多磺酸盐定量，为食品部门可靠的多磺酸盐监测方法的统一和标准化提供了支持。

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引用次数: 0

Nanozyme activity of silver nanowires for colorimetric detection of bisphenol A following salting-out assisted liquid-liquid extraction 盐析辅助液-液萃取比色法检测双酚A的银纳米线纳米酶活性研究

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-30 DOI: 10.1016/j.talo.2025.100587

Huda Salem AlSalem , Sara Naif Alharbi , Mohamed A Abdel-Lateef , Rabeea D. Abdel-Rahim , Wesam M. El-Koussi

The increasing use of industrial chemicals has raised major concerns about their environmental and health impacts, with bisphenol A (BPA) being one of the most widely studied contaminants. In this work, we introduce a proof-of-concept approach that combines the peroxidase-like catalytic activity of silver nanowires (AgNWs) with a salting-out assisted liquid–liquid extraction (SALLE) strategy for BPA detection. AgNWs catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in an acidic environment, producing a measurable color change that is effectively suppressed in the presence of BPA. This inhibition enables a simple colorimetric quantification of BPA with a detection limit of 31.62 ng/mL (LOQ = 95.83 ng/mL). The method demonstrated a practical linear response within the range of 200–700 ng/mL, making it suitable for screening moderately contaminated water samples. Furthermore, the work offers good recovery values for the extraction of BPA from spiked environmental water samples. Integrating AgNWs nanoenzyme activity with an eco-friendly pre-concentration step provides a promising low-cost, straightforward, and rapid alternative to conventional chromatographic methods, particularly suited for preliminary screening of contaminated waters.

越来越多的工业化学品的使用引起了人们对其环境和健康影响的关注，双酚A （BPA）是研究最广泛的污染物之一。在这项工作中，我们介绍了一种概念验证方法，该方法将银纳米线（AgNWs）的过氧化物酶样催化活性与盐析辅助液液萃取（SALLE）策略相结合，用于BPA检测。AgNWs在酸性环境中催化3,3 ',5,5 ' -四甲基联苯胺（TMB）的氧化，产生可测量的颜色变化，这种变化在BPA存在下被有效抑制。该抑制作用可实现双酚a的简单比色定量，检出限为31.62 ng/mL （LOQ = 95.83 ng/mL）。该方法在200 ~ 700 ng/mL范围内具有良好的线性响应，适用于中度污染水样的筛选。此外，该工作为从加标环境水样中提取BPA提供了良好的回收率值。将AgNWs纳米酶活性与生态友好的预浓缩步骤相结合，为传统色谱方法提供了一种低成本、简单、快速的替代方法，特别适合于污染水的初步筛选。

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引用次数: 0

Application of a cadmium-free continuous flow analysis system to industrial wastewater: Interference evaluation and environmental impact assessment 无镉连续流分析系统在工业废水中的应用：干扰评价与环境影响评价

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-27 DOI: 10.1016/j.talo.2025.100585

Satoshi Morikubo , Nodoka Takahashi , Takashi Nishimura , Yasuhiko Takuma , Daisuke Enomoto , Yorihiro Kumazawa , Ryosei Kanno , Suguru Okunishi , Hiroto Maeda

Building on our previous work with natural waters, this study evaluates a cadmium-free copper–zinc reduction continuous flow analysis (CFA) system for the determination of nitrogen species—nitrate-nitrogen, ammonia-nitrogen, and total nitrogen—in industrial wastewater. A key novelty is the systematic investigation of interference from common coexisting substances in industrial effluents, such as heavy metals and organic compounds, which often compromise colorimetric detection in complex matrices. We conducted controlled spike-recovery experiments and validated results with reference materials to assess analytical robustness. The cadmium-free CFA system demonstrated analytical accuracy of ≥95 % when tested with reference materials and maintained recovery rates above 90 % in actual industrial wastewater samples across all nitrogen species, even under high-interference conditions, matching the performance of conventional cadmium-based methods. Additionally, a life cycle assessment (LCA) revealed substantial reductions in environmental and human health impacts by replacing cadmium with zinc and phenol with salicylic acid, with significant improvements in toxicity and resource-related categories. These findings demonstrate that the proposed CFA method provides both analytical reliability and environmental sustainability, confirming its suitability as a robust and eco-friendly alternative for accurate nitrogen monitoring in complex industrial matrices.

基于我们之前对自然水体的研究，本研究评估了一种无镉铜锌还原连续流分析（CFA）系统，用于测定工业废水中的氮种类——硝酸盐氮、氨氮和总氮。一个关键的新颖之处在于系统地研究工业废水中常见共存物质的干扰，如重金属和有机化合物，这些物质通常会损害复杂基质中的比色检测。我们进行了对照峰回收率实验，并用参考物质验证了结果，以评估分析的稳健性。无镉CFA系统在使用标准物质进行测试时，分析精度≥95%，在实际工业废水样品中，即使在高干扰条件下，也能保持90%以上的回收率，与传统的基于镉的方法的性能相匹配。此外，生命周期评估（LCA）显示，通过用锌代替镉和用水杨酸代替苯酚，环境和人类健康影响大幅减少，毒性和与资源有关的类别也有显著改善。这些发现表明，所提出的CFA方法提供了分析可靠性和环境可持续性，证实了其作为复杂工业基质中精确氮监测的稳健和环保替代方案的适用性。

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引用次数: 0

Design and fabrication of microfluidic chip and integrated nucleic acid rapid diagnosis device 微流控芯片及集成核酸快速诊断装置的设计与制造

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-24 DOI: 10.1016/j.talo.2025.100582

Wenju Ren , Chenyang Qi , Rui Yang , Jie Zhou , Liu Yang , Yuandi Yu , Taixiong Zheng ([email protected]) , Lizhi Fu

African Swine Fever (ASF) poses a severe threat to the global pig husbandry. In the absence of an effective vaccine, developing rapid and accurate diagnostic methods is crucial for epidemic control. This study designed a low-cost microfluidic chip and integrated nucleic acid rapid detection device (INARDD) based on colorimetric principles combined with magnetic bead based nucleic acid extraction technology. Manufactured using micro computer numerical control machine tools (CNC) and 3D printing, the device fully automates the entire process include nucleic acid extraction, transfer, loop-mediated isothermal amplification (LAMP), and detection without requiring external equipment or manual intervention. Experimental results demonstrate high sensitivity (94.1 %, 32/34), specificity (97.7 %, 42/43), and accuracy (96.1 %, 74/77), with a detection limit of 10² copies/μL. The device features low production costs (approximately $69.1), low per-test expenses, and a portable design (2 kg), facilitating field deployment. This study provides an economical and efficient solution for rapid on-site diagnosis of African swine fever, holding significant implications for enhancing epidemic control and ensuring meat safety. It also offers technical reference for developing portable diagnostic systems for other infectious diseases.

非洲猪瘟（ASF）对全球养猪业构成严重威胁。在缺乏有效疫苗的情况下，开发快速和准确的诊断方法对于流行病控制至关重要。本研究设计了一种基于比色法原理结合磁珠核酸提取技术的低成本微流控芯片及集成核酸快速检测装置（INARDD）。该设备采用微型计算机数控机床（CNC）和3D打印制造，完全自动化了整个过程，包括核酸提取、转移、环介导等温扩增（LAMP）和检测，无需外部设备或人工干预。实验结果表明，该方法灵敏度为94.1%(32/34)，特异度为97.7%(42/43)，准确度为96.1%(74/77)，检出限为10²copies/μL。该设备的特点是生产成本低（约69.1美元），每次测试费用低，便携式设计（2公斤），便于现场部署。本研究为非洲猪瘟现场快速诊断提供了一种经济高效的解决方案，对加强疫情控制和确保肉类安全具有重要意义。为开发其他传染病便携式诊断系统提供了技术参考。

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引用次数: 0

Lateral flow biosensor-assisted loop-mediated isothermal amplification assay for ultrafast, sensitive detection of African swine fever virus 横向流动生物传感器辅助环介导等温扩增法超快速、灵敏检测非洲猪瘟病毒

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-21 DOI: 10.1016/j.talo.2025.100581

Fengming Chen , Sha Mao , Renjun Zhang , Xinggui Yang , Junfei Huang , Yingqian Kang , Feng Hong , Hong Chen , Shijun Li , Yi Wang

African swine fever (ASF) is an acute and highly lethal infectious disease caused by African swine fever virus (ASFV). ASF has caused huge economic losses in many countries, and there is still a lack of effective vaccines and treatments. Therefore, the development of rapid and accurate detection methods is crucial for the effective prevention and control of ASF. Here, we developed a novel loop-mediated isothermal amplification (LAMP) combined with nanoparticle-based lateral flow biosensor (LFB) for rapid and specific ASFV detection (ASFV-LAMP-LFB). The ASFV-LAMP-LFB assay was optimized to allow LAMP-LFB results to be visually verified by LFB within 2–5 min, with the entire detection process completed in 35 min and achieving a sensitivity of 0.04 fg/µL. Concurrently, the specificity of the ASFV-LAMP-LFB assay was evaluated using a range of pathogens, yielding 100 % specificity and no cross-reactivity with other porcine pathogens. In addition, the ASFV-LAMP-LFB assay exhibited a 100 % degree of sensitivity and specificity in simulated samples. In conclusion, the ASFV-LAMP-LFB assay established in this study is highly sensitive and specific, and can rapidly and accurately identify ASFV. This study offers a technical complement and support for the detection of ASFV in slaughterhouses, farms, and other grassroots units.

非洲猪瘟（ASF）是由非洲猪瘟病毒（ASFV）引起的一种急性、高致命性传染病。非洲猪瘟在许多国家造成了巨大的经济损失，目前仍缺乏有效的疫苗和治疗方法。因此，发展快速、准确的检测方法对有效防控非洲猪瘟至关重要。在这里，我们开发了一种新的环介导等温扩增（LAMP）结合纳米颗粒为基础的横向流动生物传感器（LFB）快速和特异性检测ASFV （ASFV-LAMP-LFB）。优化ASFV-LAMP-LFB实验，使LAMP-LFB结果在2-5 min内通过LFB直观验证，整个检测过程在35 min内完成，灵敏度达到0.04 fg/µL。同时，使用一系列病原体对ASFV-LAMP-LFB检测的特异性进行了评估，其特异性为100%，且与其他猪病原体无交叉反应。此外，ASFV-LAMP-LFB检测在模拟样品中表现出100%的灵敏度和特异性。综上所述，本研究建立的ASFV- lamp - lfb检测具有较高的敏感性和特异性，能够快速准确地鉴定ASFV。本研究为屠宰场、农场和其他基层单位的非洲猪瘟病毒检测提供了技术补充和支持。

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引用次数: 0

Development of molecularly imprinted polymers via dual polymerisation strategies for targeted isolation of Ethyl p-Methoxycinnamate from Kaempferia galanga L. extract 双聚合技术在山柰提取物中对甲氧基肉桂酸乙酯分子印迹聚合物中的应用

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-18 DOI: 10.1016/j.talo.2025.100580

Marisa Dwi Ariani , Ade Zuhrotun , Panagiotis Manesiotis , Aliya Nur Hasanah

The rhizome of Kaempferia galanga L. contained ethyl p-methoxycinnamate (EPMC) as a major component of its essential oil. Despite the abundance of EPMC in the plant, conventional isolation methods yielded only 0.5 – 2.5 %. This study developed molecularly imprinted polymers (MIPs) via bulk and suspension polymerisation to enhance the selective isolation of EPMC from K. galanga extracts. Six functional monomers were screened for their binding affinity with EPMC. 2-hydroxyethyl methacrylate (HEMA) in chloroform and methacrylic acid (MAA) in n-hexane were selected for further investigation. Stoichiometric analysis established optimal template-to-monomer ratios of 1:6 for HEMA and 1:7 for MAA. Eight MIP formulations and their corresponding non-imprinted polymers (NIPs), were synthesised using these monomers via both polymerisation methods. Characterisation using Fourie Transform Infra-Red (FTIR), Scanning Electron Microscope (SEM), Brunauer-Emmett-Teller (BET), and Particle Size Analysis (PSA) revealed that bulk polymers exhibited larger, irregular, and non-uniform particles compared to those produced by suspension polymerisation. Adsorption studies confirmed that the MIPs follow Freundlich isotherms, with MIP B2 (bulk, MAA, 1:7 ratio) exhibiting the highest binding affinity (KF = 0.081 mg/g). MIP B2 also demonstrated superior performance in the solid-phase extraction of EPMC from extracts, achieving recoveries of up to 82.4 % ± 5.52 and imprinting factors above 1.3. Selectivity tests confirmed strong discrimination of EPMC over structural analogues. In conclusion, MIP B2 offers a selective, efficient, and scalable method for EPMC isolation. These findings supported the continued development of tailored MIPs for natural product purification and provided a foundation for future optimisation of monomer-initiator systems and polymerisation parameters.

山柰根茎精油的主要成分是对甲氧基肉桂酸乙酯（EPMC）。尽管EPMC在植物中含量丰富，但传统的分离方法只能得到0.5 - 2.5%。为了提高高良姜提取物中EPMC的选择性分离，研究了分子印迹聚合物（MIPs）的制备方法。筛选了6个功能单体与EPMC的结合亲和力。以氯仿中的2-甲基丙烯酸羟乙酯（HEMA）和正己烷中的甲基丙烯酸（MAA）为研究对象。化学计量学分析确定HEMA和MAA的最佳模板-单体比分别为1:6和1:7。利用这些单体通过两种聚合方法合成了8种MIP配方及其相应的非印迹聚合物（NIPs）。利用傅里变换红外（FTIR）、扫描电镜（SEM）、布鲁诺尔-埃米特-泰勒（BET）和粒度分析（PSA）进行表征表明，与悬浮聚合产生的聚合物相比，大块聚合物表现出更大、不规则和不均匀的颗粒。吸附研究证实，MIP符合Freundlich等温线，其中MIP B2（体积，MAA， 1:7比）具有最高的结合亲和力（KF = 0.081 mg/g）。MIP B2固相萃取EPMC的回收率高达82.4%±5.52，印迹因子在1.3以上。选择性试验证实了EPMC对结构类似物的强烈区分。总之，MIP B2提供了一种选择性、高效和可扩展的EPMC分离方法。这些发现支持了用于天然产物纯化的定制mip的持续发展，并为未来优化单体引发剂系统和聚合参数提供了基础。

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引用次数: 0

The surface plasmon resonance biosensor capability for detection avian influenza H5N1 in poultry using immunoglobulin yolk as bioreceptor 以免疫球蛋白蛋黄为生物受体的表面等离子体共振生物传感器检测家禽H5N1禽流感的能力

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-14 DOI: 10.1016/j.talo.2025.100577

Armanda Dwi Prayugo , Wahyu Widayat , Toto Subroto , Wyanda Arnafia , Muhammad Yusuf , Gilang Gumilar , Yusuf Farid Achmad , Betty Sundari , Aulia Khoirun Nissa , Denniswara Sibit

Avian influenza is a viral infectious disease that causes high mortality and morbidity in poultry. The highly pathogenic avian influenza (HPAI) threat causes considerable losses to broiler and laying hens. Early detection of avian influenza cases on farms is now difficult, especially in laying hens with symptoms of decreased egg production, which could be caused by various other diseases. The detection of avian influenza, according to WOAH recommendations, utilizes real-time RT-PCR. The RT-PCR methods are costly, require sample extraction and laboratory expertise. Not only are sensitive and specific alternative diagnostic methods required to address the future threat of avian influenza in poultry with large populations, but also producing biomaterial as a bioreceptor on a large scale is required. Immunoglobulin yolk (IgY) is an antibody produced by the hen and found in egg yolk. Producing IgY requires hyperimmune chickens obtained through the vaccination process. The ability of IgY to bind to antigens has the potential to serve as a bioreceptor for disease detection methods in biosensor devices. A surface plasmon resonance (SPR) biosensor can detect interactions between two molecules, such as antibody-antigens, indicating a change in the resonance angle as a response unit (r.u.). The purpose of this study is to investigate the capability of the SPR biosensor for the diagnosis of AI H5N1 through its limit of detection and sensitivity using IgY anti-avian influenza H5N1, as well as the SPR specificity response in detecting avian influenza in field sample simulations. SPR development using the IgY anti-avian influenza H5N1 bioreceptor with a concentration of 50 µg/ml has a detection limit value of virus titer AI H5N1 10^4,4 ELD50/ml and a response sensitivity of 3.90 ΔRU/Log HAU with an R² value of the linear plot from antigen concentration 1 - 16 HAU is 0.99052. IgY anti-avian influenza H5N1 with a concentration of 50 µg/ml in nanoSPR8 device has a reasonable specificity of 89 % and selectivity in capturing H5N1 analyte targets. The SPR results are also promising for AI H5N1 rapid detection without extraction due to its positive response in field samples that confirmed AI H5N1 by RT-PCR.

禽流感是一种病毒性传染病，在家禽中引起高死亡率和发病率。高致病性禽流感（HPAI）的威胁给肉鸡和蛋鸡造成相当大的损失。现在很难在农场早期发现禽流感病例，特别是在产蛋量减少的蛋鸡中，这可能是由各种其他疾病引起的。根据世界卫生组织的建议，禽流感的检测利用实时RT-PCR。RT-PCR方法成本高昂，需要样品提取和实验室专业知识。不仅需要灵敏和特异的替代诊断方法来应对未来在大量家禽中出现的禽流感威胁，而且还需要大规模生产作为生物受体的生物材料。卵黄免疫球蛋白（IgY）是母鸡产生的一种抗体，存在于蛋黄中。生产IgY需要通过疫苗接种过程获得的高免疫鸡。IgY与抗原结合的能力有可能作为生物传感器设备中疾病检测方法的生物受体。表面等离子体共振（SPR）生物传感器可以检测两个分子之间的相互作用，如抗体-抗原，表明共振角的变化作为响应单位（r.u.）。本研究的目的是通过IgY抗禽流感H5N1的检测限和敏感性，探讨SPR生物传感器诊断AI H5N1的能力，以及SPR在现场样本模拟中检测禽流感的特异性反应。使用浓度为50µg/ml的IgY抗禽流感H5N1生物受体开发SPR，病毒滴度为AI H5N1 104,4 ELD50/ml，反应灵敏度为3.90 ΔRU/Log HAU，抗原浓度1 ~ 16 HAU线性图的R2值为0.99052。在nanoSPR8装置中，浓度为50µg/ml的IgY抗禽流感H5N1具有89%的合理特异性和捕获H5N1分析物目标的选择性。SPR结果也有望用于无需提取的禽流感H5N1快速检测，因为它在通过RT-PCR确认禽流感H5N1的现场样本中具有阳性反应。

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