Pub Date : 2025-01-09DOI: 10.1016/j.talo.2025.100402
Ruchika Thayil, Saidi Reddy Parne
Nanomaterials, known for their unique chemical and physical properties at the nanoscale, are crucial in advancing various cutting-edge applications. The distinct size-dependent behaviors and surface characteristics set them apart. Among these materials, molybdenum disulfide (MoS2) has gained considerable interest for room-temperature gas detection due to its high surface area, improved reactivity from the surface, and excellent carrier mobility, which makes it effective in sensing applications. In this work, MoS2 with excess sodium molybdate dihydrate (MoS2(Mo)) and MoS2 with excess thioacetamide (MoS2(S)) were synthesized using the hydrothermal method and tested for toluene gas sensing at various concentrations. The results indicate that MoS2(S) showed ΔR/Rair% of 343 % compared to MoS2(Mo), which is 197 % for 20 ppm toluene gas. In addition, MoS2(S) showed reduced response/recovery time. The results suggest that these MoS2-based sensors can be used for the development of room temperature sensors for toluene sensing.
{"title":"Tuning MoS2 nanostructures for superior room-temperature toluene sensing","authors":"Ruchika Thayil, Saidi Reddy Parne","doi":"10.1016/j.talo.2025.100402","DOIUrl":"10.1016/j.talo.2025.100402","url":null,"abstract":"<div><div>Nanomaterials, known for their unique chemical and physical properties at the nanoscale, are crucial in advancing various cutting-edge applications. The distinct size-dependent behaviors and surface characteristics set them apart. Among these materials, molybdenum disulfide (MoS<sub>2</sub>) has gained considerable interest for room-temperature gas detection due to its high surface area, improved reactivity from the surface, and excellent carrier mobility, which makes it effective in sensing applications. In this work, MoS<sub>2</sub> with excess sodium molybdate dihydrate (MoS<sub>2</sub>(Mo)) and MoS<sub>2</sub> with excess thioacetamide (MoS<sub>2</sub>(S)) were synthesized using the hydrothermal method and tested for toluene gas sensing at various concentrations. The results indicate that MoS<sub>2</sub>(S) showed ΔR/R<sub>air</sub>% of 343 % compared to MoS<sub>2</sub>(Mo), which is 197 % for 20 ppm toluene gas. In addition, MoS<sub>2</sub>(S) showed reduced response/recovery time. The results suggest that these MoS<sub>2</sub>-based sensors can be used for the development of room temperature sensors for toluene sensing.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100402"},"PeriodicalIF":4.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-07DOI: 10.1016/j.talo.2025.100401
Momen Sahriar Shoshi , Md Abu Huraiya , Vinoth Raj R , Abror Jawad , Chang Yi Kong , Hitoshi Tabata , Sankar Ganesh Ramaraj , S.M. Abdur Razzak
This study represents a new design for an empathetic surface plasmon resonance (SPR) sensor that utilizes BlueP/TMDCs and BaTiO3 (barium titanate) and employs the angular interrogation technique to measure the glucose concentration level in urine samples. This design optimization involved extensive numerical analysis through the use of the Transfer Matrix Method (TMM) at a visible wavelength of λ=633 nm to enhance sensitivity, full width half maximum (FWHM), detection accuracy (DA), and quality factor (QF). Additionally, Finite Element Method (FEM) analysis was used to ensure the accuracy of the findings achieved through Transfer Matrix Method (TMM). The suggested biosensor configuration involves five layers: a BK-7 glass prism, 56 nm Ag layer, 11 nm BaTiO3 layer, BlueP/TMDCs with thickness of 0.68 nm, and a sensing medium (urine sample). Unlike earlier designs relying on conventional 2D materials or single dielectric layers, the proposed hybrid structure not only enhances light-matter interaction and optimizes electromagnetic field distribution but also outperforms other hybrid sensors by achieving superior sensitivity, detection accuracy, and quality factor. Through the use of novel combination of BlueP/TMDCs and BaTiO₃ in this hybrid sensor, the biosensor achieved significantly improved performance, with enhanced sensitivity of 435 deg/RIU at a concentration of glucose 10 g/dL, QF of 86.29442 RIU−1 as well as DA of 0.190114 deg−1 spanning a refractive index (RI) from 1.335 to 1.347.
{"title":"Revolutionary barium titanate-BlueP/TMDCs SPR sensor: Ultra-sensitive detection of urine glucose levels","authors":"Momen Sahriar Shoshi , Md Abu Huraiya , Vinoth Raj R , Abror Jawad , Chang Yi Kong , Hitoshi Tabata , Sankar Ganesh Ramaraj , S.M. Abdur Razzak","doi":"10.1016/j.talo.2025.100401","DOIUrl":"10.1016/j.talo.2025.100401","url":null,"abstract":"<div><div>This study represents a new design for an empathetic surface plasmon resonance (SPR) sensor that utilizes BlueP/TMDCs and BaTiO<sub>3</sub> (barium titanate) and employs the angular interrogation technique to measure the glucose concentration level in urine samples. This design optimization involved extensive numerical analysis through the use of the Transfer Matrix Method (TMM) at a visible wavelength of λ=633 nm to enhance sensitivity, full width half maximum (FWHM), detection accuracy (DA), and quality factor (QF). Additionally, Finite Element Method (FEM) analysis was used to ensure the accuracy of the findings achieved through Transfer Matrix Method (TMM). The suggested biosensor configuration involves five layers: a BK-7 glass prism, 56 nm Ag layer, 11 nm BaTiO<sub>3</sub> layer, BlueP/TMDCs with thickness of 0.68 nm, and a sensing medium (urine sample). Unlike earlier designs relying on conventional 2D materials or single dielectric layers, the proposed hybrid structure not only enhances light-matter interaction and optimizes electromagnetic field distribution but also outperforms other hybrid sensors by achieving superior sensitivity, detection accuracy, and quality factor. Through the use of novel combination of BlueP/TMDCs and BaTiO₃ in this hybrid sensor, the biosensor achieved significantly improved performance, with enhanced sensitivity of 435 deg/RIU at a concentration of glucose 10 g/dL, QF of 86.29442 RIU<sup>−1</sup> as well as DA of 0.190114 deg<sup>−1</sup> spanning a refractive index (RI) from 1.335 to 1.347.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100401"},"PeriodicalIF":4.1,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-04DOI: 10.1016/j.talo.2025.100400
Ting Liang , Min Luo , Ling Shi , Hongping Yang , Guangming Yang
In this work, a nanocomposite of molybdenum disulfide (MoS₂) and graphene (r-GO) was synthesized using hydrothermal method, and was subsequently employed as a dopant to fabricate a hybrid coating of polypyrrole (PPy)-r-GO-MoS₂ via cyclic voltammetry (CV). The resulting coating was applied to extract five phenolic compounds, and combined with gas chromatography-mass spectrometry (GC–MS) to establish an analytical method for the determination of 3‑chloro-4-fluorophenol, 2‑chloro-4-methoxyphenol, 2,6-dimethoxyphenol, 2,4,6-trichlorophenol, and 2,6-dichloro-4-nitrophenol in environmental samples. The results indicated that PPy-MoS₂-r-GO coating exhibited superior extraction efficiency in comparison with to PPy coating. To optimize testing conditions, MoS₂-r-GO concentration for the electrochemical preparation of the coating, extraction conditions, including time, temperature, and stirring speed, were also investigated. Consequently, the analytical method exhibited an excellent linear response, with a correlation coefficient ranging from 0.9950 to 0.9980 for the five phenolic compounds in the range of 0.01 μg L⁻¹ to 50 μg L⁻¹. The detection limits were 0.00550 μg L⁻¹ - 0.00850 μg L⁻¹. This method was employed to detect phenol in real samples, yielding recoveries ranging from 89.60 % to 103.30 %, thereby confirming its practical applicability.
{"title":"Electrochemical preparation of polypyrrole-molybdenum disulfide-graphene nanocomposite coating for the determination of phenols","authors":"Ting Liang , Min Luo , Ling Shi , Hongping Yang , Guangming Yang","doi":"10.1016/j.talo.2025.100400","DOIUrl":"10.1016/j.talo.2025.100400","url":null,"abstract":"<div><div>In this work, a nanocomposite of molybdenum disulfide (MoS₂) and graphene (r-GO) was synthesized using hydrothermal method, and was subsequently employed as a dopant to fabricate a hybrid coating of polypyrrole (PPy)-r-GO-MoS₂ via cyclic voltammetry (CV). The resulting coating was applied to extract five phenolic compounds, and combined with gas chromatography-mass spectrometry (GC–MS) to establish an analytical method for the determination of 3‑chloro-4-fluorophenol, 2‑chloro-4-methoxyphenol, 2,6-dimethoxyphenol, 2,4,6-trichlorophenol, and 2,6-dichloro-4-nitrophenol in environmental samples. The results indicated that PPy-MoS₂-r-GO coating exhibited superior extraction efficiency in comparison with to PPy coating. To optimize testing conditions, MoS₂-r-GO concentration for the electrochemical preparation of the coating, extraction conditions, including time, temperature, and stirring speed, were also investigated. Consequently, the analytical method exhibited an excellent linear response, with a correlation coefficient ranging from 0.9950 to 0.9980 for the five phenolic compounds in the range of 0.01 μg L⁻¹ to 50 μg L⁻¹. The detection limits were 0.00550 μg L⁻¹ - 0.00850 μg L⁻¹. This method was employed to detect phenol in real samples, yielding recoveries ranging from 89.60 % to 103.30 %, thereby confirming its practical applicability.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100400"},"PeriodicalIF":4.1,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-02DOI: 10.1016/j.talo.2025.100398
Yunxing Lu , Haihui Wang , Zhou Zeng , Jianan Hui , Jiangyu Ji , Hongju Mao , Qiang Shi , Xiaoyue Yang
Exosomes are nanoscale lipid-bound vesicles secreted by various types of parent cells into the extracellular environment. They carry a wide range of bioactive molecules and serve as a crucial role in intercellular communication and tumor progression. Here, we develop an integrated microfluidic system for on-chip exosome isolation and quantum dot-based tumor marker analysis. This system integrates exosome processing and marker abundance analysis within a centimeter-scaled microfluidic chip, eliminating the need for additional off-chip treatments. We also implement YOLO v8-based image identification for sensitive and automatic detection, reducing the limit of detection (LOD) to 8.65 per microliter while minimizing manual measurement errors. Using this system, two tumor markers among four cell lines were profiled in parallel, revealing unique tumor burdens and demonstrating strong consistency with approved serological marker testing. These results highlight the potential of this technique for sensitive, precise, and automatic exosome tumor detection, paving the way for early cancer diagnosis and analysis.
{"title":"A deep learning-based integrated analytical system for tumor exosome on-chip isolation and automated image identification","authors":"Yunxing Lu , Haihui Wang , Zhou Zeng , Jianan Hui , Jiangyu Ji , Hongju Mao , Qiang Shi , Xiaoyue Yang","doi":"10.1016/j.talo.2025.100398","DOIUrl":"10.1016/j.talo.2025.100398","url":null,"abstract":"<div><div>Exosomes are nanoscale lipid-bound vesicles secreted by various types of parent cells into the extracellular environment. They carry a wide range of bioactive molecules and serve as a crucial role in intercellular communication and tumor progression. Here, we develop an integrated microfluidic system for on-chip exosome isolation and quantum dot-based tumor marker analysis. This system integrates exosome processing and marker abundance analysis within a centimeter-scaled microfluidic chip, eliminating the need for additional off-chip treatments. We also implement YOLO v8-based image identification for sensitive and automatic detection, reducing the limit of detection (LOD) to 8.65 per microliter while minimizing manual measurement errors. Using this system, two tumor markers among four cell lines were profiled in parallel, revealing unique tumor burdens and demonstrating strong consistency with approved serological marker testing. These results highlight the potential of this technique for sensitive, precise, and automatic exosome tumor detection, paving the way for early cancer diagnosis and analysis.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100398"},"PeriodicalIF":4.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-02DOI: 10.1016/j.talo.2025.100399
Dong Wei , Hao Zhang , Mingyang Li , Liuxi Chu
Circulating extracellular vehicles (EVs) play a crucial role in intercellular communication by transporting a diverse array of biological signaling molecules. Growing evidence indicates that phosphoproteins within these EVs hold promise as biomarkers for diagnostic and prognostic assessments, particularly in oncology. In this study, EVtrap (extracellular vesicle total recovery and purification) was utilized to isolate urinary EVs. We then combined this with an advanced liquid chromatography-tandem mass spectrometry (LC−MS/MS) technique to perform neutral loss-information-dependent acquisition-enhanced product ion (NL-IDA-EPI) analysis. This technique is specifically tailored to quantify endogenous phosphopeptides in urinary EVs by monitoring a consistent mass loss of 49 Da (H3PO4, doubly charged state) in the MS2 spectra. In this study, over 2,000 putative phosphopeptide signals were detected in a 10-mL urine sample without the need for prior phosphopeptide enrichment, thus simplifying the process and potentially minimizing sample bias. Comparative analysis of samples from high- and low-risk prostate cancer patients and a healthy control group revealed six differentially expressed phosphopeptides. These phosphopeptides serve as a promising panel of potential biomarkers, highlighting the effectiveness of our analytical framework in tracking cancer-related signaling events via phosphorylation within EVs using NL-IDA-EPI.
{"title":"A novel strategy for biomarker discovery: Neutral loss-dependent acquisition of circulating extracellular vesicles for the detection of phosphopeptides","authors":"Dong Wei , Hao Zhang , Mingyang Li , Liuxi Chu","doi":"10.1016/j.talo.2025.100399","DOIUrl":"10.1016/j.talo.2025.100399","url":null,"abstract":"<div><div>Circulating extracellular vehicles (EVs) play a crucial role in intercellular communication by transporting a diverse array of biological signaling molecules. Growing evidence indicates that phosphoproteins within these EVs hold promise as biomarkers for diagnostic and prognostic assessments, particularly in oncology. In this study, EVtrap (extracellular vesicle total recovery and purification) was utilized to isolate urinary EVs. We then combined this with an advanced liquid chromatography-tandem mass spectrometry (LC−MS/MS) technique to perform neutral loss-information-dependent acquisition-enhanced product ion (NL-IDA-EPI) analysis. This technique is specifically tailored to quantify endogenous phosphopeptides in urinary EVs by monitoring a consistent mass loss of 49 Da (H<sub>3</sub>PO<sub>4</sub>, doubly charged state) in the MS2 spectra. In this study, over 2,000 putative phosphopeptide signals were detected in a 10-mL urine sample without the need for prior phosphopeptide enrichment, thus simplifying the process and potentially minimizing sample bias. Comparative analysis of samples from high- and low-risk prostate cancer patients and a healthy control group revealed six differentially expressed phosphopeptides. These phosphopeptides serve as a promising panel of potential biomarkers, highlighting the effectiveness of our analytical framework in tracking cancer-related signaling events via phosphorylation within EVs using NL-IDA-EPI.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100399"},"PeriodicalIF":4.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-26DOI: 10.1016/j.talo.2024.100395
Aashutosh Dube , Shweta J. Malode , Abdullah N. Alodhayb , Kunal Mondal , Nagaraj P. Shetti
Conducting polymers are promising due to their unique properties, such as excellent electrical and optical properties, physical and chemical stability, high conductivity, and effective redox properties with high-temperature stability and biocompatibility. Due to these properties, conducting polymers are useful in diverse applications like sensors, batteries, oil industries, biosensors, biomedicines, catalysis, cancer treatment, etc. This review article aims to discuss the recent trends and analysis of conducting polymer-based electrochemical sensors in diverse areas with all required sensor characteristics, such as the derived limit of detection, utilized techniques for the sensing analysis and derived linear dynamic range with the stability of the sensors. Conducting polymers and their nanocomposites-based electrochemical sensors have demonstrated exceptional capabilities towards detecting various biomolecules, heavy metals, pesticides, and viruses like SARS-COV-2. Incorporation of redox mediators, use of conducting hydrogels, and molecular imprinting are promising strategies for better performance of the derived sensor. The article has demonstrated the existing challenges and limitations and provided solutions in the field. In the future, conducting polymers-based electrochemical sensors can be utilized in wearable sensors and integrated with IoT devices for better reach in real-time applications. They can also be made more accessible with precise control and data output by following specific methodologies. Utilizing green and sustainable conducting polymers can be crucial in advancing eco-friendly practices in the future. Conducting polymer-based electrochemical sensors has affectivity in neurochemical and pathogen sensing, which is essential for brain function and mental health.
{"title":"Conducting polymer-based electrochemical sensors: Progress, challenges, and future perspectives","authors":"Aashutosh Dube , Shweta J. Malode , Abdullah N. Alodhayb , Kunal Mondal , Nagaraj P. Shetti","doi":"10.1016/j.talo.2024.100395","DOIUrl":"10.1016/j.talo.2024.100395","url":null,"abstract":"<div><div>Conducting polymers are promising due to their unique properties, such as excellent electrical and optical properties, physical and chemical stability, high conductivity, and effective redox properties with high-temperature stability and biocompatibility. Due to these properties, conducting polymers are useful in diverse applications like sensors, batteries, oil industries, biosensors, biomedicines, catalysis, cancer treatment, etc. This review article aims to discuss the recent trends and analysis of conducting polymer-based electrochemical sensors in diverse areas with all required sensor characteristics, such as the derived limit of detection, utilized techniques for the sensing analysis and derived linear dynamic range with the stability of the sensors. Conducting polymers and their nanocomposites-based electrochemical sensors have demonstrated exceptional capabilities towards detecting various biomolecules, heavy metals, pesticides, and viruses like SARS-COV-2. Incorporation of redox mediators, use of conducting hydrogels, and molecular imprinting are promising strategies for better performance of the derived sensor. The article has demonstrated the existing challenges and limitations and provided solutions in the field. In the future, conducting polymers-based electrochemical sensors can be utilized in wearable sensors and integrated with IoT devices for better reach in real-time applications. They can also be made more accessible with precise control and data output by following specific methodologies. Utilizing green and sustainable conducting polymers can be crucial in advancing eco-friendly practices in the future. Conducting polymer-based electrochemical sensors has affectivity in neurochemical and pathogen sensing, which is essential for brain function and mental health.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100395"},"PeriodicalIF":4.1,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Metal-organic frameworks (MOFs) have emerged as promising electrode modifiers in electrochemical sensing owing to their unique structural attributes, such as high surface area, tunable porosity, high catalytic activity, and abundant active sites. These properties make MOF-based systems highly effective for detecting a wide range of analytes, including heavy metals, antibiotics, environmental pollutants, and biomarkers. MOFs offer rapid, cost-effective analysis, yet challenges remain in optimizing their electrochemical properties to fully meet the demands of practical applications, particularly in energy conversion and storage (e.g., supercapacitors, batteries, and water-splitting catalysts) and in the fabrication of high-performance electrochemical sensors. This review critically examines the electrochemical properties of MOF-based materials for detecting various analytes, exploring their current limitations and potential for improvement. Particular focus is given to the design and synthesis strategies that enhance MOFs' structural and electrochemical properties, making them more suitable for real-world applications. Furthermore, this review highlights the challenges associated with MOF stability and conductivity and suggests pathways for overcoming these barriers. Reviewing recent advancements in MOF synthesis and functionality, this article provides a comprehensive overview of how MOFs can be developed as next-generation electrochemical sensing platforms. It also discusses future perspectives, including integrating MOFs into multifunctional sensors and their potential role in wearable and IoT-enabled devices. This review bridges the gap between theoretical research and practical applications, offering valuable insights into the future of MOF-based electrochemical technologies.
{"title":"Recent advances in metal-organic frameworks for electrochemical sensing applications","authors":"Magesh Kumar Muthukumaran , Muthukumar Govindaraj , Sakthivel Kogularasu , Balasubramanian Sriram , Bharathi Kannan Raja , Sea-Fue Wang , Guo-Ping Chang-Chien , Arockia Selvi J","doi":"10.1016/j.talo.2024.100396","DOIUrl":"10.1016/j.talo.2024.100396","url":null,"abstract":"<div><div>Metal-organic frameworks (MOFs) have emerged as promising electrode modifiers in electrochemical sensing owing to their unique structural attributes, such as high surface area, tunable porosity, high catalytic activity, and abundant active sites. These properties make MOF-based systems highly effective for detecting a wide range of analytes, including heavy metals, antibiotics, environmental pollutants, and biomarkers. MOFs offer rapid, cost-effective analysis, yet challenges remain in optimizing their electrochemical properties to fully meet the demands of practical applications, particularly in energy conversion and storage (e.g., supercapacitors, batteries, and water-splitting catalysts) and in the fabrication of high-performance electrochemical sensors. This review critically examines the electrochemical properties of MOF-based materials for detecting various analytes, exploring their current limitations and potential for improvement. Particular focus is given to the design and synthesis strategies that enhance MOFs' structural and electrochemical properties, making them more suitable for real-world applications. Furthermore, this review highlights the challenges associated with MOF stability and conductivity and suggests pathways for overcoming these barriers. Reviewing recent advancements in MOF synthesis and functionality, this article provides a comprehensive overview of how MOFs can be developed as next-generation electrochemical sensing platforms. It also discusses future perspectives, including integrating MOFs into multifunctional sensors and their potential role in wearable and IoT-enabled devices. This review bridges the gap between theoretical research and practical applications, offering valuable insights into the future of MOF-based electrochemical technologies.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100396"},"PeriodicalIF":4.1,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-25DOI: 10.1016/j.talo.2024.100397
Chen-Xi Zhao, Xiao-Xia Li, Yang Shu
Abnormal alkaline phosphatase levels have been associated with several diseases, including tumor growth, diabetes, liver dysfunction and so on. Generally, the occurrence and prognosis of these diseases can be reflected by the serum alkaline phosphatase level. In this work, Eu/DBM@SiO2@SiO2 is prepared to protect the internal Eu complex from the external environment by wrapping Eu/DBM inside the silica shell. Then, Eu/DBM@SiO2@SiO2/MnO2, a sensor consists of Eu/DBM@SiO2@SiO2 and MnO2, is prepared and applied for alkaline phosphatase detection. MnO2 is used as the response part of alkaline phosphatase, which can be degraded to Mn2+ by ascorbic acid. Ascorbic acid is the hydrolysate of alkaline phosphatase. In the process of this reaction, UV–vis absorption of Eu/DBM@SiO2@SiO2/MnO2 is reduced and fluorescence is turned on. Alkaline phosphatase concentration is linearly correlated with the logarithmic value of fluorescence enhancement in the range of 10.0–100.0 U/L with linear equation of Lg(F/F0)=0.008C-0.030 (R2=0.99). For fluorescence method, the LOQ is 10.0 U/L and the LOD is 4.4 U/L. For colorimetric method, the concentration of alkaline phosphatase is linearly correlated with the decrease of absorption intensity in the range of 20.0–90.0 U/L with the linear equation of A/A0=-0.012C+1.148 (R2=0.99). The LOQ is 20.0 U/L and the LOD is 7.2 U/L. The detection system has good selectivity and can detect alkaline phosphatase in human serum samples, the accuracy of the detection system is verified by the experiment of spike-in experiment. The RSD of alkaline phosphatase concentration in serum samples is in the range of 1.4–8.4 %. This method has potential application in the detection of ALP activity in biological samples.
{"title":"Europium-based core-shell materials for fluorescence and colorimetric dual-mode sensing of alkaline phosphatase activity","authors":"Chen-Xi Zhao, Xiao-Xia Li, Yang Shu","doi":"10.1016/j.talo.2024.100397","DOIUrl":"10.1016/j.talo.2024.100397","url":null,"abstract":"<div><div>Abnormal alkaline phosphatase levels have been associated with several diseases, including tumor growth, diabetes, liver dysfunction and so on. Generally, the occurrence and prognosis of these diseases can be reflected by the serum alkaline phosphatase level. In this work, Eu/DBM@SiO<sub>2</sub>@SiO<sub>2</sub> is prepared to protect the internal Eu complex from the external environment by wrapping Eu/DBM inside the silica shell. Then, Eu/DBM@SiO<sub>2</sub>@SiO<sub>2</sub>/MnO<sub>2</sub>, a sensor consists of Eu/DBM@SiO<sub>2</sub>@SiO<sub>2</sub> and MnO<sub>2</sub>, is prepared and applied for alkaline phosphatase detection. MnO<sub>2</sub> is used as the response part of alkaline phosphatase, which can be degraded to Mn<sup>2+</sup> by ascorbic acid. Ascorbic acid is the hydrolysate of alkaline phosphatase. In the process of this reaction, UV–vis absorption of Eu/DBM@SiO<sub>2</sub>@SiO<sub>2</sub>/MnO<sub>2</sub> is reduced and fluorescence is turned on. Alkaline phosphatase concentration is linearly correlated with the logarithmic value of fluorescence enhancement in the range of 10.0–100.0 U/L with linear equation of Lg(F/F<sub>0</sub>)=0.008C-0.030 (R<sup>2</sup>=0.99). For fluorescence method, the LOQ is 10.0 U/L and the LOD is 4.4 U/L. For colorimetric method, the concentration of alkaline phosphatase is linearly correlated with the decrease of absorption intensity in the range of 20.0–90.0 U/L with the linear equation of A/A<sub>0</sub>=-0.012C+1.148 (R<sup>2</sup>=0.99). The LOQ is 20.0 U/L and the LOD is 7.2 U/L. The detection system has good selectivity and can detect alkaline phosphatase in human serum samples, the accuracy of the detection system is verified by the experiment of spike-in experiment. The RSD of alkaline phosphatase concentration in serum samples is in the range of 1.4–8.4 %. This method has potential application in the detection of ALP activity in biological samples.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100397"},"PeriodicalIF":4.1,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-20DOI: 10.1016/j.talo.2024.100394
Harshita Rai , Kshitij RB Singh , Arunadevi Natarajan , Shyam S. Pandey
This review article embarks on an enlightening journey through the multifaceted realm of electronic devices and their applications in biosensing, emphasizing the role of Field effect transistor (FET) based biosensors and Complementary Metal Oxide Semiconductor (CMOS) processes in biosensing device development. It begins by elucidating the foundational principles of biosensing and underscoring the crucial contribution of transducers, establishing a robust understanding of the field. The article unravels the intricate interplay between electronic biosensors and CMOS processes, offering a concise yet insightful exploration of their operational intricacies, diverse practical applications, and recent advancements. Additionally, it spotlights the pivotal role of FET-based biosensors integrated with CMOS processes in miniaturizing biosensors and thus amplifying their real-world efficacy. Moreover, the role of modern technologies, such as the Internet of Things (IoT), in recent biosensor development has been discussed. By addressing inherent challenges like sensitivity, integration, cost, and accessibility, the article underscores the vital role of biosensing technologies driven by electronic devices in wearable technology development. In addition, integrating these devices to fit with the ongoing trend of VLSI technology faces significant challenges. To overcome this aspect, sensors based on molecularly imprinted polymers (MIPs) can be the best alternative, as they will avoid utilizing bioreceptors, as it simplifies integration by reducing complexity, enhancing stability, and improving compatibility with CMOS processes. Hence, this review's distinct contribution lies in its comprehensive approach, shedding light on how biosensing technologies, underpinned by electronic devices such as FETs and CMOS processes, offer solutions for realizing modern-day devices.
{"title":"Advances in field effect transistor based electronic devices integrated with CMOS technology for biosensing","authors":"Harshita Rai , Kshitij RB Singh , Arunadevi Natarajan , Shyam S. Pandey","doi":"10.1016/j.talo.2024.100394","DOIUrl":"10.1016/j.talo.2024.100394","url":null,"abstract":"<div><div>This review article embarks on an enlightening journey through the multifaceted realm of electronic devices and their applications in biosensing, emphasizing the role of Field effect transistor (FET) based biosensors and Complementary Metal Oxide Semiconductor (CMOS) processes in biosensing device development. It begins by elucidating the foundational principles of biosensing and underscoring the crucial contribution of transducers, establishing a robust understanding of the field. The article unravels the intricate interplay between electronic biosensors and CMOS processes, offering a concise yet insightful exploration of their operational intricacies, diverse practical applications, and recent advancements. Additionally, it spotlights the pivotal role of FET-based biosensors integrated with CMOS processes in miniaturizing biosensors and thus amplifying their real-world efficacy. Moreover, the role of modern technologies, such as the Internet of Things (IoT), in recent biosensor development has been discussed. By addressing inherent challenges like sensitivity, integration, cost, and accessibility, the article underscores the vital role of biosensing technologies driven by electronic devices in wearable technology development. In addition, integrating these devices to fit with the ongoing trend of VLSI technology faces significant challenges. To overcome this aspect, sensors based on molecularly imprinted polymers (MIPs) can be the best alternative, as they will avoid utilizing bioreceptors, as it simplifies integration by reducing complexity, enhancing stability, and improving compatibility with CMOS processes. Hence, this review's distinct contribution lies in its comprehensive approach, shedding light on how biosensing technologies, underpinned by electronic devices such as FETs and CMOS processes, offer solutions for realizing modern-day devices.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100394"},"PeriodicalIF":4.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-18DOI: 10.1016/j.talo.2024.100393
Abdullah H. Alluhayb , Alaa M. Younis , Alaa S. Amin , Naglaa Mohamed
An environmentally sustainable optode has been developed for the detection of copper ions in water-based solutions. The optode was created by immobilizing 2-[6-nitro-2-benzothiazolylazo]-4-hydroxybenzoic acid (NBTHB) onto a porous cellulosic polymer membrane. This immobilization technique is especially beneficial for dye molecules that tend to degrade in alkaline ethylenediamine solutions, which are frequently employed during optode fabrication. The optode demonstrated a linear response to copper(II) ions within the concentration range of 10 to 130 ng mL–1, achieving a high correlation coefficient of 0.9990. The quantification and detection limits were determined to be 10 and 3.0 ng mL–1, respectively. The precision results were reported as % RSD, with intra-day values of 2.30% and 1.65%, and inter-day values of 2.15% and 1.85%, respectively. The selectivity of the sensor membrane was rigorously tested for a various cations and anions, successfully establishing the tolerance limits for interfering species. It could be effectively regenerated by treatment with 0.1 M EDTA, restoring its functionality for repeated use. The optode was effectively employed to measure copper(II) ions in a range of food products, biological fluids, and environmental water samples. The results obtained were comparable to those achieved using the ICP-AES technique, demonstrating the optodes reliability and accuracy.
{"title":"Designing an innovative, eco-conscious optode for spectrophoto-metric detection of copper across various biological and environmental samples","authors":"Abdullah H. Alluhayb , Alaa M. Younis , Alaa S. Amin , Naglaa Mohamed","doi":"10.1016/j.talo.2024.100393","DOIUrl":"10.1016/j.talo.2024.100393","url":null,"abstract":"<div><div>An environmentally sustainable optode has been developed for the detection of copper ions in water-based solutions. The optode was created by immobilizing 2-[6-nitro-2-benzothiazolylazo]-4-hydroxybenzoic acid (NBTHB) onto a porous cellulosic polymer membrane. This immobilization technique is especially beneficial for dye molecules that tend to degrade in alkaline ethylenediamine solutions, which are frequently employed during optode fabrication. The optode demonstrated a linear response to copper(II) ions within the concentration range of 10 to 130 ng mL<sup>–1</sup>, achieving a high correlation coefficient of 0.9990. The quantification and detection limits were determined to be 10 and 3.0 ng mL<sup>–1</sup>, respectively. The precision results were reported as % RSD, with intra-day values of 2.30% and 1.65%, and inter-day values of 2.15% and 1.85%, respectively. The selectivity of the sensor membrane was rigorously tested for a various cations and anions, successfully establishing the tolerance limits for interfering species. It could be effectively regenerated by treatment with 0.1 M EDTA, restoring its functionality for repeated use. The optode was effectively employed to measure copper(II) ions in a range of food products, biological fluids, and environmental water samples. The results obtained were comparable to those achieved using the ICP-AES technique, demonstrating the optodes reliability and accuracy.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100393"},"PeriodicalIF":4.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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