Talanta Open最新文献_第3页

Lemon zest-derived N,S-doped carbon quantum dots as a highly sensitive and sustainable fluorescent probe for cefoxitin sodium determination 柠檬皮衍生的N， s掺杂碳量子点作为高灵敏度和可持续荧光探针用于头孢西丁钠的测定

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 DOI: 10.1016/j.talo.2025.100601

Ahmed M. Abdel-Raoof , Ebrahim A. El-Desouky , Ahmed M. Abdelzaher , Reem H. Obaydo

Cefoxitin (CEF), classified as a semisynthetic beta-lactam second-generation cephalosporin antibiotic, can be used against many aerobic and anaerobic bacterial infections. Elevated aspartate aminotransferase levels and a higher prevalence of eosinophilia have been linked to higher CEF dosages, which alleviates the demand for ultra-sensitive determination and monitoring of its concentration level. Sulfur and nitrogen doped carbon quantum dots (N,S-CQDs) were synthesized by microwave techniques using lemon zest as a natural biomass source for green modified CQDs synthesis. N,S-CQDs used as a highly sensitive fluorescent probe, green and economic, for the determination of CEF owing to its excellent photoluminescence (PL) characters and exhibited higher quantum yield (QY) ∼37.65 % with high aqueous stability. The quenching mechanism is meticulously demonstrated to be a static quenching process through the utilization of fluorescence spectroscopy and Stern-Volmer analysis across various temperature conditions. This work developed a new method for rapid determination of CEF residues by static quenching mechanism with a detection limit (LOD) value of 0.023 µM within a linearity of 0.1–15.0 µM. Moreover, the proposed fluorescence probe has potential practical application for CEF determination in pharmaceutical dosage forms and biological fluids. Furthermore, four green evaluation tools, the Complex Modified Green Analytical Procedure Index (ComplexMoGAPI), the blue applicability grade index (BAGI), the carbon footprint reduction index (CaFRI), and a Modified Green Star Area (MoGSA), were evaluated to assess the proposed analytical method. The Evaluation and Performance of Practicality Index (EPPI, 2025) assessment demonstrated that the developed green N,S-CQDs-based fluorometric method for CEF determination is a more sustainable, efficient, and practical analytical approach compared to the reported derivatization method.

头孢西丁（CEF）是一种半合成β -内酰胺类第二代头孢菌素类抗生素，可用于治疗多种需氧和厌氧细菌感染。升高的天冬氨酸转氨酶水平和嗜酸性粒细胞增多与较高的CEF剂量有关，这减轻了对其浓度水平的超灵敏测定和监测的需求。以柠檬皮为原料，利用微波技术合成了硫氮掺杂碳量子点（N,S-CQDs）。由于N，S-CQDs具有优异的光致发光（PL）特性，具有较高的量子产率（QY） ~ 37.65%和较高的水稳定性，因此可作为一种绿色、经济的高灵敏度荧光探针用于测定CEF。通过利用荧光光谱和斯特恩-沃尔默分析，在各种温度条件下，精心证明了猝灭机制是一个静态猝灭过程。本文建立了静态猝灭法快速测定CEF残留量的新方法，在0.1 ~ 15.0µM的线性范围内，检出限为0.023µM。此外，所提出的荧光探针在药物剂型和生物液体中CEF的测定具有潜在的实际应用价值。此外，本文还对复杂修正绿色分析程序指数（ComplexMoGAPI）、蓝色适用性等级指数（BAGI）、碳足迹减少指数（CaFRI）和修正绿星面积（MoGSA） 4种绿色评价工具进行了评价。实用指数评估和性能（EPPI, 2025）评估表明，与已有的衍生化方法相比，所开发的基于绿色N， s- cqds的荧光法测定CEF是一种更可持续、更高效、更实用的分析方法。

{"title":"Lemon zest-derived N,S-doped carbon quantum dots as a highly sensitive and sustainable fluorescent probe for cefoxitin sodium determination","authors":"Ahmed M. Abdel-Raoof , Ebrahim A. El-Desouky , Ahmed M. Abdelzaher , Reem H. Obaydo","doi":"10.1016/j.talo.2025.100601","DOIUrl":"10.1016/j.talo.2025.100601","url":null,"abstract":"<div><div>Cefoxitin (CEF), classified as a semisynthetic beta-lactam second-generation cephalosporin antibiotic, can be used against many aerobic and anaerobic bacterial infections. Elevated aspartate aminotransferase levels and a higher prevalence of eosinophilia have been linked to higher CEF dosages, which alleviates the demand for ultra-sensitive determination and monitoring of its concentration level. Sulfur and nitrogen doped carbon quantum dots (N,S-CQDs) were synthesized by microwave techniques using lemon zest as a natural biomass source for green modified CQDs synthesis. N,S-CQDs used as a highly sensitive fluorescent probe, green and economic, for the determination of CEF owing to its excellent photoluminescence (PL) characters and exhibited higher quantum yield (QY) ∼37.65 % with high aqueous stability. The quenching mechanism is meticulously demonstrated to be a static quenching process through the utilization of fluorescence spectroscopy and Stern-Volmer analysis across various temperature conditions. This work developed a new method for rapid determination of CEF residues by static quenching mechanism with a detection limit (LOD) value of 0.023 µM within a linearity of 0.1–15.0 µM. Moreover, the proposed fluorescence probe has potential practical application for CEF determination in pharmaceutical dosage forms and biological fluids. Furthermore, four green evaluation tools, the Complex Modified Green Analytical Procedure Index (ComplexMoGAPI), the blue applicability grade index (BAGI), the carbon footprint reduction index (CaFRI), and a Modified Green Star Area (MoGSA), were evaluated to assess the proposed analytical method. The Evaluation and Performance of Practicality Index <em>(EPPI, 2025)</em> assessment demonstrated that the developed green N,S-CQDs-based fluorometric method for CEF determination is a more sustainable, efficient, and practical analytical approach compared to the reported derivatization method.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100601"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of veterinary antibiotics in German poultry manure by ultrasonic assisted extraction and QuEChERS coupled with LC-MS/MS 超声辅助提取- QuEChERS - LC-MS/MS联合测定德国家禽粪中兽用抗生素

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-12-01 DOI: 10.1016/j.talo.2025.100592

Svenja Nathalie Weber , Nadin Ulrich , Jana Mühlenberg , Nils Engler , Michael Nelles

A fast and efficient analytical method for the quantification of antibiotic residues in poultry manure based on ultrasonic assisted extraction (UAE) and QuEChERS cleanup followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. It was successfully validated for amoxicillin, cefaclor, ciprofloxacin, clarithromycin, erythromycin, lincomycin, metronidazole and ofloxacin in terms of linearity, detection and quantification limit, recovery, intra-day and inter-day precision and matrix effect. The overall method performance showed good linearity and recoveries ranging between 80.6 % and 138.1 % with sufficient limits of quantification for the targeted analytes. The method was applied to eleven poultry manure samples. Lincomycin and ciprofloxacin were detected in eight of eleven samples. The results show that the developed fast and efficient method can be employed to analyze poultry manure samples. In addition, antibiotic residues can be found regularly in poultry manure samples. When used in biogas plants or as fertilizer, they can pose a risk to human health and the environment. The method also includes cefaclor, an antibiotic that has, to the best of our knowledge, not yet been analysed in poultry manure.

建立了一种基于超声辅助提取（UAE）和QuEChERS清洗-液相色谱-串联质谱（LC-MS/MS）的快速高效家禽粪便中抗生素残留定量分析方法。对阿莫西林、头孢氯、环丙沙星、克拉霉素、红霉素、林可霉素、甲硝唑和氧氟沙星的线性度、检测定量限、回收率、日内、日间精密度和基质效应进行了验证。该方法线性良好，加样回收率在80.6% ~ 138.1%之间，具有足够的定量限。该方法应用于11份禽粪样品。11份样品中有8份检出林可霉素和环丙沙星。结果表明，该方法可用于禽粪样品的快速、高效分析。此外，在家禽粪便样本中经常可以发现抗生素残留。当用于沼气厂或用作肥料时，它们可能对人类健康和环境构成风险。这种方法还包括头孢克洛，据我们所知，这种抗生素还没有在家禽粪便中进行过分析。

{"title":"Determination of veterinary antibiotics in German poultry manure by ultrasonic assisted extraction and QuEChERS coupled with LC-MS/MS","authors":"Svenja Nathalie Weber , Nadin Ulrich , Jana Mühlenberg , Nils Engler , Michael Nelles","doi":"10.1016/j.talo.2025.100592","DOIUrl":"10.1016/j.talo.2025.100592","url":null,"abstract":"<div><div>A fast and efficient analytical method for the quantification of antibiotic residues in poultry manure based on ultrasonic assisted extraction (UAE) and QuEChERS cleanup followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed. It was successfully validated for amoxicillin, cefaclor, ciprofloxacin, clarithromycin, erythromycin, lincomycin, metronidazole and ofloxacin in terms of linearity, detection and quantification limit, recovery, intra-day and inter-day precision and matrix effect. The overall method performance showed good linearity and recoveries ranging between 80.6 % and 138.1 % with sufficient limits of quantification for the targeted analytes. The method was applied to eleven poultry manure samples. Lincomycin and ciprofloxacin were detected in eight of eleven samples. The results show that the developed fast and efficient method can be employed to analyze poultry manure samples. In addition, antibiotic residues can be found regularly in poultry manure samples. When used in biogas plants or as fertilizer, they can pose a risk to human health and the environment. The method also includes cefaclor, an antibiotic that has, to the best of our knowledge, not yet been analysed in poultry manure.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100592"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145614589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of mercury in urban dust: direct sample analysis and establishment of a geochemical baseline 城市尘埃中汞的测定：直接样品分析和地球化学基线的建立

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-11-27 DOI: 10.1016/j.talo.2025.100599

Rafael R. Francisco , Nicole E.J. Santos , Leonardo S.G. Teixeira , Maria Graças A. Korn , Leandro Kolling , Morgana B. Dessuy , Rennan G.O. Araujo

Airborne particulate matter, particularly urban dust, poses health and environmental risks by gathering toxic elements. Among these, mercury (Hg) is notable for its persistence, mobility, and tendency to bioaccumulate, with human-made emissions amplifying its harmful impacts on people and ecosystems. This study examined Hg levels in urban dust samples collected from 11 cities in Bahia, Brazil, using direct solid analysis. Quantification was carried out with an external calibration curve based on certified reference material of fly ash (CRM BCR 176R). The limits of detection and quantification were 0.2 ng g⁻¹ and 0.8 ng g⁻¹, respectively. Method accuracy was validated using CRM of Trace Elements in Multi-Nutrient Fertilizer (NIST 695), which yielded a agreement of 96.5 (± 3.3) %. Precision, expressed as the relative standard deviation, was better than 3.1 % (n = 4). Hg concentrations ranged from 14.8 (± 0.5) ng g⁻¹ to 488 (± 2) ng g⁻¹, with the highest levels observed in Salvador. The average concentration was 236 (± 149) ng g⁻¹, and the median was 262 ng g⁻¹. A geochemical baseline for Hg concentration was established through linear regression with Mn concentrations, supporting the calculation of the enrichment factor (EF) and the geoaccumulation index (Igeo). EF values indicated Hg enrichment in Salvador, Jaguaquara, Camaçari, and Mata de São João. The Igeo values revealed Hg contamination in Salvador, Jaguaquara, Camaçari, Mata de São João, Dias D’Ávila, and Caetité. These results highlight human-made mercury contributions, mainly linked to vehicular traffic and urban growth in these areas, and emphasize the need for continuous environmental monitoring and effective air quality management in cities.

空气中的颗粒物，特别是城市尘埃，聚集有毒元素，对健康和环境构成风险。其中，汞（Hg）因其持久性、流动性和生物积累倾向而引人注目，人为排放放大了其对人类和生态系统的有害影响。这项研究使用直接固体分析方法检测了从巴西巴伊亚州11个城市收集的城市尘埃样本中的汞含量。采用外标曲线法对经认证的粉煤灰标准物质（CRM BCR 176R）进行定量。检测限和定量限分别为0.2 ng g⁻¹和0.8 ng g⁻¹。采用多营养肥中微量元素的CRM （NIST 695）验证了方法的准确性，一致性为96.5（±3.3）%。精密度（以相对标准偏差表示）优于3.1% （n = 4）。汞的浓度从14.8（±0.5）ng g⁻-到488（±2）ng g⁻-不等，其中萨尔瓦多的汞浓度最高。平均浓度为236（±149）ng g⁻¹，中位数为262 ng g⁻¹。通过与Mn浓度的线性回归，建立了Hg浓度的地球化学基线，支持了富集因子（EF）和地球聚集指数（Igeo）的计算。EF值显示在Salvador、Jaguaquara、camaari和Mata de s o jo o等地区Hg富集。Igeo值显示，萨尔瓦多、雅瓜奎拉、卡马帕拉塔里、Mata de s o joo、Dias D ' Ávila和caetit受到汞污染。这些结果强调了人为汞贡献，主要与这些地区的车辆交通和城市增长有关，并强调需要在城市中进行持续的环境监测和有效的空气质量管理。

{"title":"Determination of mercury in urban dust: direct sample analysis and establishment of a geochemical baseline","authors":"Rafael R. Francisco , Nicole E.J. Santos , Leonardo S.G. Teixeira , Maria Graças A. Korn , Leandro Kolling , Morgana B. Dessuy , Rennan G.O. Araujo","doi":"10.1016/j.talo.2025.100599","DOIUrl":"10.1016/j.talo.2025.100599","url":null,"abstract":"<div><div>Airborne particulate matter, particularly urban dust, poses health and environmental risks by gathering toxic elements. Among these, mercury (Hg) is notable for its persistence, mobility, and tendency to bioaccumulate, with human-made emissions amplifying its harmful impacts on people and ecosystems. This study examined Hg levels in urban dust samples collected from 11 cities in Bahia, Brazil, using direct solid analysis. Quantification was carried out with an external calibration curve based on certified reference material of fly ash (CRM BCR 176R). The limits of detection and quantification were 0.2 ng g⁻¹ and 0.8 ng g⁻¹, respectively. Method accuracy was validated using CRM of Trace Elements in Multi-Nutrient Fertilizer (NIST 695), which yielded a agreement of 96.5 (± 3.3) %. Precision, expressed as the relative standard deviation, was better than 3.1 % (<em>n</em> = 4). Hg concentrations ranged from 14.8 (± 0.5) ng g⁻¹ to 488 (± 2) ng g⁻¹, with the highest levels observed in Salvador. The average concentration was 236 (± 149) ng g⁻¹, and the median was 262 ng g⁻¹. A geochemical baseline for Hg concentration was established through linear regression with Mn concentrations, supporting the calculation of the enrichment factor (EF) and the geoaccumulation index (Igeo). EF values indicated Hg enrichment in Salvador, Jaguaquara, Camaçari, and Mata de São João. The Igeo values revealed Hg contamination in Salvador, Jaguaquara, Camaçari, Mata de São João, Dias D’Ávila, and Caetité. These results highlight human-made mercury contributions, mainly linked to vehicular traffic and urban growth in these areas, and emphasize the need for continuous environmental monitoring and effective air quality management in cities.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100599"},"PeriodicalIF":3.7,"publicationDate":"2025-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145693122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating full scan and data-dependent acquisition (IFSDDA): advancing quantitative precision and metabolic coverage in untargeted metabolomics for discovering anti-methicillin-resistant Staphylococcus aureus (MRSA) compounds 整合全扫描和数据依赖采集（IFSDDA）：推进非靶向代谢组学的定量精度和代谢覆盖，以发现抗甲氧西林耐药金黄色葡萄球菌（MRSA）化合物

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-11-19 DOI: 10.1016/j.talo.2025.100597

Wun-Yu Lin , Han-Ju Chien , Yi-Feng Zheng , Cheng-Yu Kuo , Wei-Chen Wang , Nien-Jen Hu , Yu-Sheng Lin , Chien-Chen Lai

In untargeted metabolomics, high-resolution liquid chromatography–tandem mass spectrometry (HR-LC-MS/MS) is widely applied to achieve broad metabolite coverage. However, a single acquisition mode often presents trade-offs between coverage and quantification. To overcome this, we propose a dual-injection strategy combining full scan (FS) and data-dependent acquisition (DDA) modes in consecutive runs of the same sample. The resulting datasets are processed by an in-house developed algorithm that integrates feature lists from both FS and DDA, enhancing both the number of detected features and the quantification precision. This integrated approach, named Integrated Feature Set from DDA and FS (IFSDDA), improves peak picking robustness and captures features otherwise missed by conventional methods. A total of 32,830 metabolic features were detected using IFSDDA, markedly surpassing those detected by traditional approaches. Quantitative precision was ensured through QC-based support vector regression (SVR) normalization, with 94.2% of features achieving a relative standard deviation (RSD) below 20%. For metabolite annotation, 2845 features were structurally annotated, indicating superior annotation efficiency. Notably, the IFSDDA strategy facilitated the identification of six anti-MRSA metabolites from Pseudomonas aeruginosa, including 1-carboxamide (PCN), pyocyanin (PYO), 1-hydroxyphenazine (1-HP), PQS, and 2-heptyl-4-hydroxyquinoline N-oxide (HQNO). These metabolites were subsequently validated through multiple reaction monitoring (MRM) and biological activity assays. Overall, IFSDDA effectively addresses key limitations in untargeted metabolomics by enhancing data quality, reproducibility, and metabolite annotation. This strategy represents a significant advancement for comprehensive metabolic profiling and holds strong potential for biomarker discovery in complex biological systems.

在非靶向代谢组学中，高分辨率液相色谱-串联质谱（HR-LC-MS/MS）被广泛应用于实现广泛的代谢物覆盖。然而，单一的获取模式通常会在覆盖率和量化之间进行权衡。为了克服这个问题，我们提出了一种双注入策略，结合全扫描（FS）和数据依赖采集（DDA）模式，在同一样品的连续运行中。结果数据集由内部开发的算法处理，该算法集成了FS和DDA的特征列表，提高了检测到的特征数量和量化精度。这种集成方法被称为来自DDA和FS的集成特征集（IFSDDA），提高了峰值选取的鲁棒性，并捕获了传统方法无法捕获的特征。利用IFSDDA共检测到32,830个代谢特征，明显超过传统方法。通过基于qc的支持向量回归（SVR）归一化，保证了定量精度，94.2%的特征实现了相对标准偏差（RSD）低于20%。对于代谢物的注释，2845个特征被结构注释，表明了较高的注释效率。值得注意的是，IFSDDA策略促进了铜绿假单胞菌中6种抗mrsa代谢物的鉴定，包括1-羧酰胺（PCN）、pyocyanin （PYO）、1-羟基吩嗪（1-HP）、PQS和2-庚基-4-羟基喹啉n -氧化物（HQNO）。这些代谢物随后通过多重反应监测（MRM）和生物活性测定进行验证。总体而言，IFSDDA通过提高数据质量、可重复性和代谢物注释，有效地解决了非靶向代谢组学的关键限制。该策略代表了综合代谢分析的重大进步，并在复杂生物系统中发现生物标志物具有强大的潜力。

{"title":"Integrating full scan and data-dependent acquisition (IFSDDA): advancing quantitative precision and metabolic coverage in untargeted metabolomics for discovering anti-methicillin-resistant Staphylococcus aureus (MRSA) compounds","authors":"Wun-Yu Lin , Han-Ju Chien , Yi-Feng Zheng , Cheng-Yu Kuo , Wei-Chen Wang , Nien-Jen Hu , Yu-Sheng Lin , Chien-Chen Lai","doi":"10.1016/j.talo.2025.100597","DOIUrl":"10.1016/j.talo.2025.100597","url":null,"abstract":"<div><div>In untargeted metabolomics, high-resolution liquid chromatography–tandem mass spectrometry (HR-LC-MS/MS) is widely applied to achieve broad metabolite coverage. However, a single acquisition mode often presents trade-offs between coverage and quantification. To overcome this, we propose a dual-injection strategy combining full scan (FS) and data-dependent acquisition (DDA) modes in consecutive runs of the same sample. The resulting datasets are processed by an in-house developed algorithm that integrates feature lists from both FS and DDA, enhancing both the number of detected features and the quantification precision. This integrated approach, named Integrated Feature Set from DDA and FS (IFSDDA), improves peak picking robustness and captures features otherwise missed by conventional methods. A total of 32,830 metabolic features were detected using IFSDDA, markedly surpassing those detected by traditional approaches. Quantitative precision was ensured through QC-based support vector regression (SVR) normalization, with 94.2% of features achieving a relative standard deviation (RSD) below 20%. For metabolite annotation, 2845 features were structurally annotated, indicating superior annotation efficiency. Notably, the IFSDDA strategy facilitated the identification of six anti-MRSA metabolites from <em>Pseudomonas aeruginosa</em>, including 1-carboxamide (PCN), pyocyanin (PYO), 1-hydroxyphenazine (1-HP), PQS, and 2-heptyl-4-hydroxyquinoline N-oxide (HQNO). These metabolites were subsequently validated through multiple reaction monitoring (MRM) and biological activity assays. Overall, IFSDDA effectively addresses key limitations in untargeted metabolomics by enhancing data quality, reproducibility, and metabolite annotation. This strategy represents a significant advancement for comprehensive metabolic profiling and holds strong potential for biomarker discovery in complex biological systems.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100597"},"PeriodicalIF":3.7,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual enzyme-mimicking nanoplatform based on CoMn-LDHs for ultrasensitive detection of L-cysteine: Toward environmental and food safety monitoring 基于con - ldhs的双酶模拟纳米平台超灵敏检测l -半胱氨酸：用于环境和食品安全监测

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-11-17 DOI: 10.1016/j.talo.2025.100596

Salma Shalaby , Jenny Jeehan Mohamed Nasr , Asmaa Kamal El-Deen

Cysteine (CYS) is a pivotal biomolecule involved in both biological and environmental processes, yet conventional detection methods suffer from low sensitivity, complex procedures, and interference issues. In this study, we report the development of cobalt-manganese layered double hydroxides (CoMn-LDHs) as a novel nanomaterial-based sensing platform with dual oxidase/peroxidase mimicry for label-free CYS detection. The synthesized CoMn-LDHs exhibit exceptional catalytic activity, efficiently oxidizing the chromogenic substrate TMB through the generation of reactive species such as superoxide anion (O₂•⁻) and hydroxyl radicals (•OH), which significantly enhance sensitivity and selectivity. This dual-enzyme mimic mechanism enables a rapid, colorimetric detection method with ultra-low detection limits of 0.024 μM and 0.12 μM for oxidase and peroxidase modes, respectively, outperforming traditional techniques. The sensor demonstrates excellent performance in real samples, including mineral water, tap water, and milk, with high recovery rates (> 98 %) and low relative standard deviation (%RSD < 2 %), showcasing its robust stability and reproducibility in complex matrices. This work presents a cost-effective, highly sensitive, and selective platform for CYS detection, offering significant advancements in environmental monitoring and food safety applications through the innovative use of nanomaterial-based enzyme mimics.

半胱氨酸（CYS）是一种参与生物和环境过程的关键生物分子，但传统的检测方法存在灵敏度低、程序复杂和干扰问题。在这项研究中，我们报道了钴锰层状双氢氧化物（com - ldhs）的发展，作为一种新型的基于纳米材料的传感平台，具有双氧化酶/过氧化物酶模拟，用于无标记CYS检测。合成的com - ldhs具有特殊的催化活性，通过生成超氧阴离子（O₂•毒血症）和羟基自由基（•OH）等活性物质，有效地氧化显色底物TMB，显着提高了灵敏度和选择性。该双酶模拟机制可实现氧化酶和过氧化物酶模式的快速比色检测，超低检出限分别为0.024 μM和0.12 μM，优于传统技术。该传感器在矿泉水、自来水和牛奶等实际样品中表现出优异的性能，具有高回收率（98%）和低相对标准偏差（%RSD < 2%），在复杂基质中具有良好的稳定性和重复性。这项工作提出了一个具有成本效益，高灵敏度和选择性的CYS检测平台，通过创新地使用基于纳米材料的酶模拟物，在环境监测和食品安全应用方面取得了重大进展。

{"title":"A dual enzyme-mimicking nanoplatform based on CoMn-LDHs for ultrasensitive detection of L-cysteine: Toward environmental and food safety monitoring","authors":"Salma Shalaby , Jenny Jeehan Mohamed Nasr , Asmaa Kamal El-Deen","doi":"10.1016/j.talo.2025.100596","DOIUrl":"10.1016/j.talo.2025.100596","url":null,"abstract":"<div><div>Cysteine (CYS) is a pivotal biomolecule involved in both biological and environmental processes, yet conventional detection methods suffer from low sensitivity, complex procedures, and interference issues. In this study, we report the development of cobalt-manganese layered double hydroxides (CoMn-LDHs) as a novel nanomaterial-based sensing platform with dual oxidase/peroxidase mimicry for label-free CYS detection. The synthesized CoMn-LDHs exhibit exceptional catalytic activity, efficiently oxidizing the chromogenic substrate TMB through the generation of reactive species such as superoxide anion (O₂•⁻) and hydroxyl radicals (•OH), which significantly enhance sensitivity and selectivity. This dual-enzyme mimic mechanism enables a rapid, colorimetric detection method with ultra-low detection limits of 0.024 μM and 0.12 μM for oxidase and peroxidase modes, respectively, outperforming traditional techniques. The sensor demonstrates excellent performance in real samples, including mineral water, tap water, and milk, with high recovery rates (> 98 %) and low relative standard deviation (%RSD < 2 %), showcasing its robust stability and reproducibility in complex matrices. This work presents a cost-effective, highly sensitive, and selective platform for CYS detection, offering significant advancements in environmental monitoring and food safety applications through the innovative use of nanomaterial-based enzyme mimics.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100596"},"PeriodicalIF":3.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical composition, antioxidant potential, and toxicity of infusions and decoctions of Greek mountain tea (Sideritis clandestina subsp. clandestina) from the Peloponnese 希腊山茶（Sideritis clandestina subsp.）冲剂和煎剂的植物化学成分、抗氧化潜力和毒性。秘密地从伯罗奔尼撒半岛

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-11-17 DOI: 10.1016/j.talo.2025.100595

Eleftheria H. Kaparakou , Charalabos D. Kanakis , Dimitra Daferera , Petros A. Tarantilis

Sideritis clandestina subsp. clandestina, endemic to the Peloponnese and traditionally used as a mountain tea, belongs to a group of herbal infusions widely consumed across the Mediterranean and Balkan regions. This study investigates the phytochemical composition and antioxidant potential of infusions and decoctions prepared from aerial parts collected in three Peloponnesian regions. LC/QTOF analysis identified 18 secondary metabolites, including acetylated and methylated flavonoid glycosides, phenylethanoid glycosides, phenolic acids, and diterpenes. Significant compositional variation was observed across geographic origins, with Mt. Mainalon samples exhibiting the highest total phenolic content and antioxidant activity. Decoctions consistently yielded higher concentrations of bioactive compounds than infusions. In vitro assessment using the Microtox assay showed consistent EC₅₀ values across samples, indicating measurable bioactivity without raising safety concerns. These findings highlight S. clandestina subsp. clandestina as a promising natural source of antioxidants for nutraceutical applications, with bioactivity influenced by both extraction method and geographic provenance.

隐性黄疸秘密茶，伯罗奔尼撒特有，传统上用作山茶，属于一组草药冲剂，在地中海和巴尔干地区广泛使用。本研究研究了从伯罗奔尼撒三个地区收集的空气部位制成的浸剂和煎剂的植物化学成分和抗氧化潜力。LC/QTOF分析鉴定出18种次生代谢物，包括乙酰化和甲基化的类黄酮苷、苯乙醇苷、酚酸和二萜。在不同的地理来源中观察到显著的成分差异，其中Mt. Mainalon样品显示出最高的总酚含量和抗氧化活性。煎剂始终比输液产生更高浓度的生物活性化合物。使用Microtox法进行的体外评估显示，样品之间的EC₅0值一致，表明可测量的生物活性，而不会引起安全问题。这些发现突出了S. clandestina subsp。作为一种很有前途的天然抗氧化剂的营养保健应用，其生物活性受提取方法和地理来源的影响。

{"title":"Phytochemical composition, antioxidant potential, and toxicity of infusions and decoctions of Greek mountain tea (Sideritis clandestina subsp. clandestina) from the Peloponnese","authors":"Eleftheria H. Kaparakou , Charalabos D. Kanakis , Dimitra Daferera , Petros A. Tarantilis","doi":"10.1016/j.talo.2025.100595","DOIUrl":"10.1016/j.talo.2025.100595","url":null,"abstract":"<div><div><em>Sideritis clandestina</em> subsp. <em>clandestina</em>, endemic to the Peloponnese and traditionally used as a mountain tea, belongs to a group of herbal infusions widely consumed across the Mediterranean and Balkan regions. This study investigates the phytochemical composition and antioxidant potential of infusions and decoctions prepared from aerial parts collected in three Peloponnesian regions. LC/QTOF analysis identified 18 secondary metabolites, including acetylated and methylated flavonoid glycosides, phenylethanoid glycosides, phenolic acids, and diterpenes. Significant compositional variation was observed across geographic origins, with Mt. Mainalon samples exhibiting the highest total phenolic content and antioxidant activity. Decoctions consistently yielded higher concentrations of bioactive compounds than infusions. In vitro assessment using the Microtox assay showed consistent EC₅₀ values across samples, indicating measurable bioactivity without raising safety concerns. These findings highlight <em>S. clandestina</em> subsp. <em>clandestina</em> as a promising natural source of antioxidants for nutraceutical applications, with bioactivity influenced by both extraction method and geographic provenance.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100595"},"PeriodicalIF":3.7,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining o-DGT passive sampling with UHPLC-Q-ToF MS for the analysis of 12 tracer micropollutants in the context of the European urban wastewater directive (PE-CONS 85/1/24) 结合o-DGT被动采样和UHPLC-Q-ToF MS对欧洲城市废水指令（PE-CONS 85/1/24）背景下12种示踪微污染物的分析

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-11-14 DOI: 10.1016/j.talo.2025.100594

Mikel Bernabeu de Maria , Robin Guibal , Matthias Monneron-Gyurits , Gilles Guibaud

This study presents an integrated and innovative approach for monitoring the 12 tracer organic micropollutants introduced in the new Urban Wastewater Directive (PE-CONS 85/1/24) of the European Council. The proposed method combines the Diffusive Gradients in Thin Films (o-DGT) passive sampling with ultra-high-performance liquid chromatography coupled to high-resolution time-of-flight mass spectrometry (UHPLC-Q-ToF MS). The analytical procedure was validated in terms of linearity, accuracy, and limits of quantification (LOQs), which ranged from 0.05 to 0.5 µg L⁻¹ for all compounds, except for hydrochlorothiazide (2 µg L⁻¹). Matrix interferences in o-DGT extracts (comprising agarose diffusive gel, HLB binding phase, and cellulose acetate membrane) exposed in treated wastewater were effectively removed after evaporation and ten-fold dilution, ensuring reliable quantification of target compounds. Following laboratory calibration, with the determination of five new diffusion coefficients, o-DGT devices were deployed at three sites along a river, including the urban wastewater treatment plant (WWTP) discharge channel. The combination of passive sampling and the developed analytical method enabled the detection of all 12 target compounds in WWTP treated waters and revealed an increased micropollutant concentrations in downstream site. Beyond confirming the method robustness and field applicability, o-DGT proved to be more sensitive and reliable than commonly used grab sampling, which failed to quantify certain compounds at low concentration in water. Moreover, o-DGT allowed the estimation of time-weighted average (TWA) concentrations and fluxes, representing a powerful tool for future monitoring of the efficiency of WWTP to remove micropollutant from water.

本研究提出了一种综合和创新的方法来监测欧洲理事会新城市废水指令（PE-CONS 85/1/24）中引入的12种示踪有机微污染物。该方法将薄膜扩散梯度（o-DGT）被动采样与超高效液相色谱耦合高分辨率飞行时间质谱（UHPLC-Q-ToF MS）相结合。该分析方法在线性、准确性和定量限（loq）方面得到了验证，除氢氯噻嗪（2µg L⁻¹）外，所有化合物的定量范围为0.05至0.5µg L⁻¹。暴露在处理过的废水中的o-DGT提取物（包括琼脂糖扩散凝胶、HLB结合相和醋酸纤维素膜）在蒸发和10倍稀释后有效去除基质干扰，确保了目标化合物的可靠定量。在实验室校准后，确定了五个新的扩散系数，o-DGT装置被部署在河流沿岸的三个地点，包括城市污水处理厂（WWTP）排放通道。被动采样与开发的分析方法相结合，可以检测到污水处理厂处理水中所有12种目标化合物，并显示下游站点微污染物浓度增加。除了证实了方法的稳健性和现场适用性外，o-DGT被证明比常用的抓取采样更敏感和可靠，后者无法定量水中低浓度的某些化合物。此外，o-DGT允许估计时间加权平均（TWA）浓度和通量，是未来监测污水处理厂去除水中微污染物效率的有力工具。

{"title":"Combining o-DGT passive sampling with UHPLC-Q-ToF MS for the analysis of 12 tracer micropollutants in the context of the European urban wastewater directive (PE-CONS 85/1/24)","authors":"Mikel Bernabeu de Maria , Robin Guibal , Matthias Monneron-Gyurits , Gilles Guibaud","doi":"10.1016/j.talo.2025.100594","DOIUrl":"10.1016/j.talo.2025.100594","url":null,"abstract":"<div><div>This study presents an integrated and innovative approach for monitoring the 12 tracer organic micropollutants introduced in the new Urban Wastewater Directive (PE-CONS 85/1/24) of the European Council. The proposed method combines the Diffusive Gradients in Thin Films (o-DGT) passive sampling with ultra-high-performance liquid chromatography coupled to high-resolution time-of-flight mass spectrometry (UHPLC-Q-ToF MS). The analytical procedure was validated in terms of linearity, accuracy, and limits of quantification (LOQs), which ranged from 0.05 to 0.5 µg L⁻¹ for all compounds, except for hydrochlorothiazide (2 µg L⁻¹). Matrix interferences in o-DGT extracts (comprising agarose diffusive gel, HLB binding phase, and cellulose acetate membrane) exposed in treated wastewater were effectively removed after evaporation and ten-fold dilution, ensuring reliable quantification of target compounds. Following laboratory calibration, with the determination of five new diffusion coefficients, o-DGT devices were deployed at three sites along a river, including the urban wastewater treatment plant (WWTP) discharge channel. The combination of passive sampling and the developed analytical method enabled the detection of all 12 target compounds in WWTP treated waters and revealed an increased micropollutant concentrations in downstream site. Beyond confirming the method robustness and field applicability, o-DGT proved to be more sensitive and reliable than commonly used grab sampling, which failed to quantify certain compounds at low concentration in water. Moreover, o-DGT allowed the estimation of time-weighted average (TWA) concentrations and fluxes, representing a powerful tool for future monitoring of the efficiency of WWTP to remove micropollutant from water.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100594"},"PeriodicalIF":3.7,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical immunosensor based on screen-printed electrodes modified with gold nanoparticles for highly sensitive detection of TGF-α 基于金纳米粒子修饰的丝网印刷电极的电化学免疫传感器用于TGF-α的高灵敏度检测

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-11-05 DOI: 10.1016/j.talo.2025.100590

Smriti Gaba, Utkarsh Jain

Transforming Growth Factor Alpha (TGF-α) is a critical biomarker associated with various malignancies and pathological conditions, including cancer and other diseases. For efficient diagnosis and therapeutic monitoring, TGF-α in clinical serum must be detected accurately. In this study, we present a highly sensitive and selective electrochemical immunosensor for the detection of TGF-α, leveraging the principles of immunorecognition and advanced signal transduction. The immunosensor was constructed by immobilizing monoclonal anti-TGF-α antibodies via EDCNHS chemistry onto a screen-printed electrode surface modified with gold nanoparticles to enhance electron transfer and provide a high surface area for antibody binding. Gold nanomaterials were characterized by UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS), and Transmission electron microscopy (TEM) to confirm their morphology and size. Cyclic voltammetry (CV) and X-ray photoelectron spectroscopy (XPS) were employed to characterize the electrode modifications, ensuring stability and functional integrity. The developed sensor demonstrated excellent analytical performance, with a detection limit of 0.35 pg mL⁻¹, a linear range of 1–1000 pg mL⁻¹, and a high sensitivity of 0.051 mA. The immunosensor demonstrated high specificity, with negligible interference from other serum proteins, and robust repeatability. The sensor retained about 98% of its original response after storage at 4°C for 1 week, demonstrating acceptable long-term stability of the functionalized platform. This study underscores the potential of electrochemical immunosensors as a promising platform for the point-of-care diagnostics of TGF-α, enabling timely and accurate disease management. Future advancements may integrate multiplex detection capabilities and real serum analysis for broader clinical applicability and real-world applications.

转化生长因子α (TGF-α)是一种重要的生物标志物，与各种恶性肿瘤和病理状况相关，包括癌症和其他疾病。为了有效的诊断和治疗监测，必须准确检测临床血清TGF-α。在本研究中，我们利用免疫识别和高级信号转导的原理，提出了一种高灵敏度和选择性的检测TGF-α的电化学免疫传感器。该免疫传感器通过EDCNHS化学将单克隆抗tgf -α抗体固定在用金纳米颗粒修饰的丝网印刷电极表面上，以增强电子传递并为抗体结合提供高表面积。利用紫外可见光谱（UV-Vis）、傅里叶变换红外光谱（FTIR）、动态光散射（DLS）和透射电子显微镜（TEM）对金纳米材料进行了表征，以确定其形貌和尺寸。利用循环伏安法（CV）和x射线光电子能谱法（XPS）对电极修饰进行了表征，确保了电极的稳定性和功能完整性。该传感器具有良好的分析性能，检测限为0.35 pg mL - 1，线性范围为1-1000 pg mL - 1，灵敏度为0.051 mA。该免疫传感器具有高特异性，可忽略其他血清蛋白的干扰，并且具有良好的重复性。在4°C下储存1周后，传感器保留了约98%的原始响应，表明功能化平台具有可接受的长期稳定性。这项研究强调了电化学免疫传感器作为TGF-α即时诊断平台的潜力，从而实现及时准确的疾病管理。未来的进展可能会整合多重检测能力和真实血清分析，以获得更广泛的临床适用性和现实应用。

{"title":"Electrochemical immunosensor based on screen-printed electrodes modified with gold nanoparticles for highly sensitive detection of TGF-α","authors":"Smriti Gaba, Utkarsh Jain","doi":"10.1016/j.talo.2025.100590","DOIUrl":"10.1016/j.talo.2025.100590","url":null,"abstract":"<div><div>Transforming Growth Factor Alpha (TGF-α) is a critical biomarker associated with various malignancies and pathological conditions, including cancer and other diseases. For efficient diagnosis and therapeutic monitoring, TGF-α in clinical serum must be detected accurately. In this study, we present a highly sensitive and selective electrochemical immunosensor for the detection of TGF-α, leveraging the principles of immunorecognition and advanced signal transduction. The immunosensor was constructed by immobilizing monoclonal anti-TGF-α antibodies via EDC<img>NHS chemistry onto a screen-printed electrode surface modified with gold nanoparticles to enhance electron transfer and provide a high surface area for antibody binding. Gold nanomaterials were characterized by UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS), and Transmission electron microscopy (TEM) to confirm their morphology and size. Cyclic voltammetry (CV) and X-ray photoelectron spectroscopy (XPS) were employed to characterize the electrode modifications, ensuring stability and functional integrity. The developed sensor demonstrated excellent analytical performance, with a detection limit of 0.35 pg mL⁻¹, a linear range of 1–1000 pg mL⁻¹, and a high sensitivity of 0.051 mA. The immunosensor demonstrated high specificity, with negligible interference from other serum proteins, and robust repeatability. The sensor retained about 98% of its original response after storage at 4°C for 1 week, demonstrating acceptable long-term stability of the functionalized platform. This study underscores the potential of electrochemical immunosensors as a promising platform for the point-of-care diagnostics of TGF-α, enabling timely and accurate disease management. Future advancements may integrate multiplex detection capabilities and real serum analysis for broader clinical applicability and real-world applications.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100590"},"PeriodicalIF":3.7,"publicationDate":"2025-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous determination of 11 haloacetic acids and oxyhalides by UPLC-HRMS in Human Serum, Breast Milk, and Urine UPLC-HRMS同时测定人血清、母乳和尿液中的11种卤乙酸和氧卤化物

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-11-04 DOI: 10.1016/j.talo.2025.100589

Zelin Wang, Xiaoyu Hu, Qiaozhen Guo, Mengdie Fan, Yang Xing, Sai Fan, Yunjia Yang, Jing Zhang, Bing Shao

Disinfection byproducts (DBPs), particularly haloacetic acids (HAAs) and oxyhalides, are of global concern due to their carcinogenicity and occurrence beyond drinking water. Biomonitoring data in human biological matrices remain scarce, especially for emerging iodinated DBPs. This study presents a validated analytical method for simultaneously quantifying 11 HAAs and oxyhalides in serum, urine, and breast milk. This method combines a tailored solid-phase clean-up with ultra-performance liquid chromatography–high-resolution mass spectrometry (UPLCHRMS) and achieves excellent recoveries of 77–117 %, low method detection limits of 0.01–0.20 μg l^-1, and acceptable matrix effects across all matrices. Applied to 122 biological samples collected in Beijing, this approach revealed several unprecedented findings: (i) mono-iodoacetic acid (MIAA), a highly toxic iodinated DBP, was consistently detected in all three matrices, marking the first such report in human biomonitoring; (ii) chlorate was detected in human urine for the first time, with a mean concentration of 117 μg l^-1, indicating overlooked exposure pathways; and (iii) significant correlations between HAAs and oxyhalides in biological samples suggest shared exposure sources or metabolic processes not previously documented. Serum exhibited the highest HAA concentrations, suggesting its high body burden. Chlorate and perchlorate were frequently detected at high concentrations in urine and breast milk, highlighting possible risks to vulnerable populations. This study addresses critical gaps in methodology and exposure data, providing a robust tool for DBP biomonitoring and key evidence for cumulative risk assessment beyond drinking water.

消毒副产物（DBPs），特别是卤代乙酸（HAAs）和氧卤化物，由于其致癌性和在饮用水之外的存在而受到全球关注。人类生物基质的生物监测数据仍然很少，特别是对于新出现的碘化dbp。本研究提出了一种有效的分析方法，用于同时定量血清、尿液和母乳中的11 HAAs和氧卤化物。该方法结合了定制的固相净化和超高效液相色谱-高分辨率质谱（UPLCHRMS），回收率为77 - 117%，方法检出限为0.01-0.20 μg -1，所有基质的基质效应均可接受。将该方法应用于北京采集的122个生物样本，揭示了几个前所未有的发现：(i)在所有三种基质中均检测到高毒性碘化DBP -单碘乙酸（MIAA），这是人类生物监测中首次报道；（ii）首次在人尿中检出氯酸盐，平均浓度为117 μg l-1，暴露途径被忽视；（iii）生物样品中HAAs和氧化卤化物之间的显著相关性表明，以前没有文献记载的共同暴露源或代谢过程。血清中HAA浓度最高，表明其机体负荷高。尿液和母乳中经常检测到高浓度氯酸盐和高氯酸盐，这突出了弱势群体可能面临的风险。本研究解决了方法和暴露数据方面的关键空白，为DBP生物监测提供了强有力的工具，并为饮用水以外的累积风险评估提供了关键证据。

{"title":"Simultaneous determination of 11 haloacetic acids and oxyhalides by UPLC-HRMS in Human Serum, Breast Milk, and Urine","authors":"Zelin Wang, Xiaoyu Hu, Qiaozhen Guo, Mengdie Fan, Yang Xing, Sai Fan, Yunjia Yang, Jing Zhang, Bing Shao","doi":"10.1016/j.talo.2025.100589","DOIUrl":"10.1016/j.talo.2025.100589","url":null,"abstract":"<div><div>Disinfection byproducts (DBPs), particularly haloacetic acids (HAAs) and oxyhalides, are of global concern due to their carcinogenicity and occurrence beyond drinking water. Biomonitoring data in human biological matrices remain scarce, especially for emerging iodinated DBPs. This study presents a validated analytical method for simultaneously quantifying 11 HAAs and oxyhalides in serum, urine, and breast milk. This method combines a tailored solid-phase clean-up with ultra-performance liquid chromatography–high-resolution mass spectrometry (UPLC<img>HRMS) and achieves excellent recoveries of 77–117 %, low method detection limits of 0.01–0.20 μg l<sup>-1</sup>, and acceptable matrix effects across all matrices. Applied to 122 biological samples collected in Beijing, this approach revealed several unprecedented findings: (i) mono-iodoacetic acid (MIAA), a highly toxic iodinated DBP, was consistently detected in all three matrices, marking the first such report in human biomonitoring; (ii) chlorate was detected in human urine for the first time, with a mean concentration of 117 μg l<sup>-1</sup>, indicating overlooked exposure pathways; and (iii) significant correlations between HAAs and oxyhalides in biological samples suggest shared exposure sources or metabolic processes not previously documented. Serum exhibited the highest HAA concentrations, suggesting its high body burden. Chlorate and perchlorate were frequently detected at high concentrations in urine and breast milk, highlighting possible risks to vulnerable populations. This study addresses critical gaps in methodology and exposure data, providing a robust tool for DBP biomonitoring and key evidence for cumulative risk assessment beyond drinking water.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100589"},"PeriodicalIF":3.7,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145516937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From carob to carbon dots: Sustainable synthesis of fluorescent nanosensor for sensitive secnidazole determination in pharmaceutical, biological, and environmental samples 从角豆到碳点：可持续合成的荧光纳米传感器用于制药、生物和环境样品中塞克尼唑的灵敏测定

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-11-04 DOI: 10.1016/j.talo.2025.100588

Raneem Hesham , Heba Abd El-Aziz , Rania El-Shaheny

A fast, one pot, eco-friendly, and affordable fluorescent nanoprobe for the antimicrobial drug secnidazole was created. Highly luminescent N-doped carbon nanoparticles (N-CNPs) were produced for the first time from carob fruit extract as a readily available precursor via microwave-assisted synthesis in about six minutes. The developed N-CNPs exhibited good stability and water solubility. EDX, TEM, XPS, XRD, Zeta potential analysis, UV-visible spectroscopy, Fourier transform infrared, and fluorescence spectroscopy were used to characterize the N-CNPs comprehensively. The furnished N-CNPs demonstrated outstanding capabilities as a fluorescence nanosensor for secnidazole detection at λ_ex/λ_em of 330/425 nm within a concentration range from 10.0 to 200.0 μM and a limit of detection of 2.8 μM. The fluorescence of the proposed N-CNPs was successfully quenched by secnidazole via the inner filter effect. The nanoprobe exhibits excellent secnidazole selectivity even when a variety of potentially interfering drugs, excipients, or ions are present. This approach was used to analyze secnidazole in pharmaceutical dosage forms with a recovery percentage of 99.52 ± 1.37 % for Fladazole tablets® and 101.6 ± 1.92 % for Cipazole tablets® and in tap and irrigation water with recovery percentages of 101.1 ± 3.2 % and 99.23 ± 1.11 %, respectively. Furthermore, the N-CNPs have been employed to monitor the urinary elimination of SNZ with a limit of detection of 6.95 μM. The use of an economical and sustainable plant as a substrate through microwave-assisted synthesis shows excellent greenness and blueness characteristics of the suggested probe as assessed by AGREE, ComplexGAPI, and BAGI, respectively.

研制了一种快速、一锅、环保、价格合理的抗微生物药物塞克硝唑荧光纳米探针。本文首次以角豆果提取物为原料，通过微波辅助合成，在6分钟内制备了高发光n掺杂碳纳米颗粒（N-CNPs）。制备的N-CNPs具有良好的稳定性和水溶性。利用EDX、TEM、XPS、XRD、Zeta电位分析、紫外-可见光谱、傅里叶变换红外光谱和荧光光谱对N-CNPs进行了全面表征。所制备的N-CNPs作为一种荧光纳米传感器，在λex/λem范围为330/425 nm，检测浓度为10.0 ~ 200.0 μM，检测限为2.8 μM。所提出的N-CNPs的荧光被塞克硝唑通过内过滤效应成功猝灭。即使存在多种潜在干扰药物、赋形剂或离子，纳米探针也表现出优异的塞硝唑选择性。采用该方法对药用剂型中的塞克硝唑进行分析，弗拉唑片®的回收率为99.52±1.37%，西帕唑片®的回收率为101.6±1.92%，自来水和灌溉水的回收率分别为101.1±3.2%和99.23±1.11%。此外，N-CNPs还被用于监测尿中SNZ的消除，检测限为6.95 μM。利用经济和可持续的植物作为底物，通过微波辅助合成表明，所建议的探针具有优异的绿色和蓝色特性，分别通过AGREE， ComplexGAPI和BAGI进行了评估。

{"title":"From carob to carbon dots: Sustainable synthesis of fluorescent nanosensor for sensitive secnidazole determination in pharmaceutical, biological, and environmental samples","authors":"Raneem Hesham , Heba Abd El-Aziz , Rania El-Shaheny","doi":"10.1016/j.talo.2025.100588","DOIUrl":"10.1016/j.talo.2025.100588","url":null,"abstract":"<div><div>A fast, one pot, eco-friendly, and affordable fluorescent nanoprobe for the antimicrobial drug secnidazole was created. Highly luminescent N-doped carbon nanoparticles (N-CNPs) were produced for the first time from carob fruit extract as a readily available precursor via microwave-assisted synthesis in about six minutes. The developed N-CNPs exhibited good stability and water solubility. EDX, TEM, XPS, XRD, Zeta potential analysis, UV-visible spectroscopy, Fourier transform infrared, and fluorescence spectroscopy were used to characterize the N-CNPs comprehensively. The furnished N-CNPs demonstrated outstanding capabilities as a fluorescence nanosensor for secnidazole detection at λ<sub>ex</sub>/λ<sub>em</sub> of 330/425 nm within a concentration range from 10.0 to 200.0 μM and a limit of detection of 2.8 μM. The fluorescence of the proposed N-CNPs was successfully quenched by secnidazole via the inner filter effect. The nanoprobe exhibits excellent secnidazole selectivity even when a variety of potentially interfering drugs, excipients, or ions are present. This approach was used to analyze secnidazole in pharmaceutical dosage forms with a recovery percentage of 99.52 ± 1.37 % for Fladazole tablets® and 101.6 ± 1.92 % for Cipazole tablets® and in tap and irrigation water with recovery percentages of 101.1 ± 3.2 % and 99.23 ± 1.11 %, respectively. Furthermore, the N-CNPs have been employed to monitor the urinary elimination of SNZ with a limit of detection of 6.95 μM. The use of an economical and sustainable plant as a substrate through microwave-assisted synthesis shows excellent greenness and blueness characteristics of the suggested probe as assessed by AGREE, ComplexGAPI, and BAGI, respectively.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100588"},"PeriodicalIF":3.7,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145516931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0