Talanta Open最新文献_第3页

A critical review of the field applications of diffusive gradients (DGT) in assessing the environmental behavior of emerging organic contaminants: Advantages, limitations, and methodological considerations 扩散梯度（DGT）在评估新出现的有机污染物的环境行为中的现场应用：优势，局限性和方法学考虑的重要回顾

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-14 DOI: 10.1016/j.talo.2025.100576

Zhou Fang , Xuan Hu , Jun Luo , Shuting Liu

As the environmental impacts of emerging organic contaminants (EOCs) gained increasing global attention, the monitoring of their concentrations and the assessment of their ecological risks have become critical areas of research. Diffusive gradients in thin films (DGT) have been increasingly applied to investigate the distribution and environmental behavior of EOCs in aquatic, soil, and sedimentary environments. This review synthesizes current advancements in DGT technology, with a particular focus on the field applications of DGTs for the studies of the distributions and behaviors of EOCs in the water bodies, soil, and sediment. The advantages, and limitations of diffusion and binding layer materials, filter membranes, and molding techniques were summarized based on the principles of DGT. The reliability and stability of DGT for EOCs determination in aquatic environments are evaluated through a comparative analysis with traditional grab sampling methods. The advantages and limitations of DGT are also discussed in relation to other passive sampling techniques. In addition, common challenges associated with DGT field deployments are identified, and potential strategies for methodological improvements are proposed. Finally, this review consolidates existing studies on the application of DGT in examining the distribution and environmental behavior of EOCs in water, soil, and sediment systems. These findings collectively underscore the utility of DGT in advancing the study of EOCs environmental behavior and in facilitating ecological risk assessment.

随着新兴有机污染物对环境的影响日益受到全球的关注，其浓度监测和生态风险评估已成为重要的研究领域。薄膜扩散梯度（DGT）越来越多地应用于研究水生、土壤和沉积环境中EOCs的分布和环境行为。本文综述了DGT技术的最新进展，重点介绍了DGT在研究水体、土壤和沉积物中生态系统的分布和行为方面的应用。根据DGT的原理，总结了扩散和结合层材料、过滤膜和成型技术的优点和局限性。通过与传统抓取采样方法的对比分析，评价了DGT在水生环境中测定EOCs的可靠性和稳定性。与其他无源采样技术相比，DGT的优点和局限性也进行了讨论。此外，还确定了与DGT现场部署相关的共同挑战，并提出了改进方法的潜在战略。最后，对DGT在水、土壤和沉积物系统中EOCs分布和环境行为研究中的应用进行了综述。这些发现共同强调了DGT在推进eoc环境行为研究和促进生态风险评估方面的作用。

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引用次数: 0

Accurate and robust method for plant volatilome analysis by GC-MS GC-MS分析植物挥发物准确可靠的方法

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-14 DOI: 10.1016/j.talo.2025.100578

Marysol Ferretti , Giuseppe Spiezia , Mariachiara Lo Scalzo , Valeria Todeschini , Guido Lingua , Eleonora Conterosito , Emilio Guerrieri , Valentina Gianotti

Plants continuously release volatile organic compounds (VOCs) belonging to distinct chemical classes, which are involved in various biological and ecological functions. Furthermore, the VOC profile contributes to the organoleptic properties of plants, influencing their commercial value in the food markets. The composition of VOCs varies with the species, the phenology and can also vary in response to different stimuli to which the plant is subjected, leading to the emission of different molecules or very subtle variations of concentration, resulting in a great variability in the collected samples. Consequently, the sample collection method and analysis must be robust and reliable, to minimize the uncertainty that affects the results. This work proposes a quantitative GC–MS method, the gold standard for identifying and quantifying VOCs molecules that was optimized and validated following the AOAC Guidelines for fifteen analytes typically present in Solanum lycopersicum (tomato plant). The focus in the validation was also addressed to the optimization of the collection procedure that is the most critical point from the point of view of the repeatability and reproducibility.

The validated method has then been applied to the study of real tomato plants volatilome, collected with the tailored and optimised setup.

The method developed in this study serves as a highly reliable analytical tool for characterizing plant volatilome. By integrating optimized protocols for GC–MS with meticulous validation processes, this approach enhances our ability to investigate plant secondary metabolism and its ecological significance.

植物不断释放的挥发性有机化合物（VOCs）属于不同的化学类别，涉及多种生物和生态功能。此外，挥发性有机化合物的特征有助于植物的感官特性，影响其在食品市场上的商业价值。挥发性有机化合物的组成随物种、物候而变化，也可能因植物受到的不同刺激而变化，导致不同分子的释放或浓度的非常细微的变化，从而导致收集到的样品有很大的变化。因此，样品采集方法和分析必须稳健可靠，以尽量减少影响结果的不确定性。本工作提出了一种定量GC-MS方法，该方法是鉴定和定量VOCs分子的金标准，并根据AOAC指南对茄茄（番茄植物）中典型的15种分析物进行了优化和验证。验证的重点还在于收集程序的优化，从可重复性和再现性的角度来看，这是最关键的一点。经过验证的方法随后被应用于研究真正的番茄植物挥发素，这些挥发素是通过定制和优化的设置收集的。本研究建立的方法是一种高度可靠的植物挥发油特征分析工具。通过将优化的GC-MS方案与细致的验证过程相结合，该方法增强了我们研究植物次生代谢及其生态意义的能力。

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引用次数: 0

Confinement of AgNCs in cholesterol-DNA micelle for improved circulating tumor DNA detection via entropy-driven circulatory strategy 通过熵驱动循环策略，限制胆固醇-DNA胶束中的agnc以改善循环肿瘤DNA检测

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-10 DOI: 10.1016/j.talo.2025.100575

Yufei Guan, Han Leng, Xuwei Chen

DNA-templated silver nanoclusters (DNA-AgNCs) have gained wide applications in biochemical analysis due to the flexible preparation and favorable fluorescence properties, while the limited photostability and the structural and functional stability remains a critical issue that warrants careful consideration during practical applications. Herein, spherical DNA micelles were constructed via the assembly of cholesterol modified DNA strand with specific sequence for ctDNA recognition and in-situ AgNCs growth. The spatial confinement of AgNCs in the micelles not only enhanced the fluorescence performance of AgNCs effectively through ordered aggregation, but also improved its photostability greatly. Sensitive ctDNA detection was achieved via entropy-driven circulatory strategy by monitoring the fluorescence variation of AgNCs. The detection limit was 6.06 pM, improved by 3 orders of magnitude compared to conventional process. Moreover, the developed detection system exhibited high tolerance to the complex matrices of biological sample, and the practical applicability was validated by accurately measuring PIK3CA E542K ctDNA contents in human serum samples.

dna模板银纳米团簇（dna - agnc）由于其灵活的制备和良好的荧光特性在生化分析中获得了广泛的应用，但其有限的光稳定性以及结构和功能稳定性仍然是实际应用中需要认真考虑的关键问题。本文通过将胆固醇修饰的DNA链组装成具有特定序列的球形DNA胶束，用于ctDNA识别和原位agnc生长。agnc在胶束中的空间约束不仅通过有序聚集有效增强了agnc的荧光性能，而且大大提高了其光稳定性。通过监测agnc的荧光变化，通过熵驱动循环策略实现了敏感的ctDNA检测。检出限为6.06 pM，比常规方法提高了3个数量级。此外，该检测系统对生物样品的复杂基质具有较高的耐受性，并通过准确测定人血清样品中PIK3CA E542K ctDNA含量验证了该检测系统的实用性。

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引用次数: 0

Simultaneous quantification and natural 13C abundance of fatty acids in breast cancer tissues and serum by GC-C-IRMS for tumor characterization GC-C-IRMS同时定量测定乳腺癌组织和血清中脂肪酸的天然13C丰度，用于肿瘤表征

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-10 DOI: 10.1016/j.talo.2025.100573

Louise Mangeon , Romain Le Balch , Olivier L. Mantha , Cyrille Guimaraes-carneiro , Michelle Pinault , Régis Hankard , Arnaud De Luca , Illa Tea

Background

Breast cancer (BrCa) is a heterogeneous disease which complicates early detection, subtyping, and treatment selection. Metabolic alterations are hallmarks of cancer, but current methods to study metabolism, especially stable isotope analysis, involve complex workflows, derivatization steps, and have limited clinical use. Lipid metabolism is altered in BrCa, yet sensitive, minimally invasive tools to quantify fatty acid (FA) isotope signatures are lacking. A gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) method allowing direct, high-precision analysis of underivatized FAs in tissues and serum is needed for improved cancer phenotyping.

Results

A novel GC-C-IRMS method is developed for direct δ¹³C (carbon isotope composition) and concentration analysis of underivatized FAs in tissues and serum, with excellent repeatability, reproducibility, and minimal matrix effects. The optimized method involves simple sample preparation by lipid hydrolysis without requiring derivatization, followed by FA separation using a polar GC column. The method is validated for both quantification and isotopic analysis using internal standards and applied to human tissue and serum samples from patients with different BrCa subtypes. Significant natural ¹³C enrichment is observed in C16:0, C16:1, and C18:1, alongside a decrease in C14:0 and C16:1 concentrations in cancerous tissue compared to adjacent non-cancerous tissue, reflecting shifts in lipid metabolism during carinogenesis. Importantly, δ¹³C values of C18:0 and C18:2 in both cancerous tissue and serum differed betwen BrCa subtypes.

Significance

This is the first GC-C-IRMS method enabling high-precision, direct analysis of underivatized FAs in tissues and serum with minimal sample preparation. The approach offers a promising, minimally invasive tool for characterizing FA metabolism and improving breast cancer detection, subtyping, and metabolic phenotyping without isotopic labeling.

乳腺癌（BrCa）是一种异质性疾病，它使早期发现、亚型分型和治疗选择变得复杂。代谢改变是癌症的标志，但目前研究代谢的方法，特别是稳定同位素分析，涉及复杂的工作流程，衍生化步骤，并且临床应用有限。脂质代谢在BrCa中发生改变，但缺乏敏感的、微创的工具来量化脂肪酸（FA）同位素特征。气相色谱-燃烧-同位素比值质谱（GC-C-IRMS）方法可以直接，高精度地分析组织和血清中未活化的FAs，这是改善癌症表型所必需的。结果建立了一种新的GC-C-IRMS方法，用于组织和血清中欠活化FAs的δ13C（碳同位素组成）和浓度的直接分析，具有良好的重复性、再现性和最小的基质效应。优化后的方法包括通过脂质水解制备样品，无需衍生化，然后使用极性气相色谱柱分离FA。使用内部标准验证了该方法的定量和同位素分析，并应用于不同BrCa亚型患者的人体组织和血清样本。在C16:0、C16:1和C18:1中观察到显著的天然13C富集，同时与邻近非癌组织相比，癌组织中C14:0和C16:1浓度降低，反映了癌变过程中脂质代谢的变化。重要的是，癌组织和血清中C18:0和C18:2的δ¹³C值在BrCa亚型之间存在差异。这是第一个GC-C-IRMS方法，可以高精度，直接分析组织和血清中未酸化的FAs，只需最少的样品制备。该方法提供了一种有前途的微创工具，用于表征FA代谢，改善乳腺癌检测、亚型分型和代谢表型，而无需同位素标记。

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引用次数: 0

A novel sampling strategy for metal ions determination in water: application to the determination of cadmium 水中金属离子测定的新取样策略：在镉测定中的应用

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-08 DOI: 10.1016/j.talo.2025.100574

Francisco A.P. Rodrigues, Tânia C.F. Ribas, Raquel B.R. Mesquita, António O.S.S. Rangel

This work proposes a novel, portable, and reusable device for in situ water sampling, sample preparation, and analyte enrichment in a single step. For this purpose, a commercial cationic exchange resin (Chelex 100), was used to obtain a solid-phase extraction column for cadmium (II) retention and enrichment. The optimization of key parameters such as sample volume, reagents concentration, and flow-rate, was performed to maximize cadmium retention and elution efficiency. The method was applied to real samples, and the results agreed with those obtained with reference methodologies, with no significant differences (< 10%). Additionally, recovery rates of 97.8 ± 3.5% were achieved. This method integrates, in one step, the sampling and sample preparation procedures and employs reusable devices, contributing to providing a sustainable solution for metal ions monitoring in aquatic environments.

这项工作提出了一种新的，便携式的，可重复使用的装置，用于原位水采样，样品制备和分析物富集在一个步骤中。为此，使用商业阳离子交换树脂（Chelex 100）获得镉（II）保留和富集的固相萃取柱。对样品体积、试剂浓度和流速等关键参数进行了优化，以最大限度地提高镉的保留和洗脱效率。将该方法应用于实际样品，结果与参考方法一致，无显著差异（< 10%）。回收率为97.8±3.5%。该方法将采样和样品制备程序整合在一起，采用可重复使用的设备，有助于为水生环境中的金属离子监测提供可持续的解决方案。

引用次数: 0

Analytical techniques and source apportionment for heavy metal(loid)s in groundwater: A comprehensive review 地下水中重金属（样态）分析技术及来源分析综述

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-02 DOI: 10.1016/j.talo.2025.100572

Jelena Vesković, Antonije Onjia

Groundwater contamination by heavy metal(loid)(HM) is a pervasive global issue threatening drinking water quality and public health. Effective mitigation requires robust analytical methodologies and predictive modeling frameworks. This review critically assesses cutting-edge instrumental techniques and source apportionment methods for detecting and understanding HM pollution in groundwater. Advanced laboratory techniques, exemplified by inductively coupled plasma mass spectrometry (ICP-MS), now achieve extraordinary sensitivity with detection limits in the sub-μg/L range and exhibit high precision (relative standard deviations between 2–3 %). Furthermore, hyphenated chromatography-spectrometry techniques, such as ion chromatography coupled to ICP-MS, facilitate detailed chemical speciation, effectively resolving redox-sensitive pairs such as Cr(III)/Cr(VI) and As(III)/As(V) at environmental concentrations (1–50 µg/L). Meanwhile, emerging electrochemical portable sensors enable on-site, real-time monitoring of groundwater quality. For source apportionment, multivariate statistical methods (including receptor models), isotopic fingerprinting, and machine learning algorithms have been increasingly integrated to disentangle complex mixtures of natural versus anthropogenic metal sources. By combining improved detection limits, enhanced speciation insights, and powerful data analytics and modeling tools, modern approaches support groundwater monitoring and pollution source tracking. Nevertheless, challenges such as matrix interferences, instability of metal species during sampling, and overlapping source signatures continue to complicate analysis, underscoring the need for integrated approaches that merge analytical innovation with data-driven tools to refine source attribution and ultimately ensure groundwater safety. Critical knowledge gaps are identified, including the need for more consistent monitoring approaches, closer integration of new analytical tools with field practice, and stronger policies to make sure scientific progress actually leads to better groundwater protection.

地下水重金属污染是一个普遍存在的全球性问题，威胁着饮用水质量和公众健康。有效的缓解需要稳健的分析方法和预测建模框架。这篇综述批判性地评估了用于检测和理解地下水中HM污染的尖端仪器技术和来源分配方法。以电感耦合等离子体质谱法（ICP-MS）为例的先进实验室技术现在实现了非凡的灵敏度，检测限在亚μg/L范围内，并具有很高的精度（相对标准偏差在2 - 3%之间）。此外，联用色谱光谱技术，如离子色谱与ICP-MS耦合，有助于详细的化学形态形成，有效地分辨环境浓度（1-50 μ g/L）下的氧化还原敏感对，如Cr(III)/Cr（VI）和as (III)/ as (V)。同时，新兴的电化学便携式传感器使现场实时监测地下水质量成为可能。对于来源分配，多元统计方法（包括受体模型）、同位素指纹和机器学习算法已经越来越多地集成在一起，以解开自然与人为金属来源的复杂混合物。通过结合改进的检测极限、增强的物种形成洞察力以及强大的数据分析和建模工具，现代方法支持地下水监测和污染源跟踪。然而，诸如基质干扰、采样过程中金属种类的不稳定性以及源特征重叠等挑战继续使分析复杂化，强调了将分析创新与数据驱动工具相结合的综合方法的必要性，以改进源属性，最终确保地下水安全。报告指出了关键的知识差距，包括需要更一致的监测方法、更紧密地将新的分析工具与实地实践结合起来，以及更强有力的政策，以确保科学进步实际上带来更好的地下水保护。

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引用次数: 0

Strategies for resin reuse in Sr/matrix separation prior to isotope analysis 同位素分析前Sr/基质分离中树脂再利用策略

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-10-02 DOI: 10.1016/j.talo.2025.100571

Agnese Aguzzoni, Giulio Voto, Werner Tirler

One of the key passages to achieve an accurate and precise Sr isotope ratio (⁸⁷Sr/⁸⁶Sr) measurement is the Sr/matrix separation. Traditionally this step is accomplished using a Sr-spec resin which allows the Sr recovery eliminating the main interferent species (namely Ca, Rb, Pb). While this application is consolidated, few details are available in literature regarding the possibility to reuse the resin for the consecutive separation of Sr from multiple samples. To deepen this aspect, in this study different tests were conducted both using certified and in-house reference materials, to check the performance of the separation in different working conditions and testing solutions of HNO₃ and HCl for the resin washing between consecutive use cycles. These tests confirmed that resin reuse guarantees average Sr recovery higher than 95 %, with a negligible presence of interfering species in the final solution, and no effect on the Sr isotope ratios. However, since in no case a quantitative Sr recovery was achieved, the presence of Sr residues on the resin might limit this application if different samples are treated on the same resin. Tests with batches of real samples showed that using a mild washing with HNO₃ (0.05 M), although residual cross-contamination was present, no significant alteration of the sample ⁸⁷Sr/⁸⁶Sr ratios occurred, in case of homogeneous ratios. At increasing levels of heterogeneity, even the use of HCl in the resin washing might be not sufficient. This study demonstrated the feasibility of resin reuse, reducing its consumption, associated costs, and lab workload, without affecting the accuracy of the results when working with sample replicates or when a low-to-medium difference in the ⁸⁷Sr/⁸⁶Sr ratio of consecutive samples is expected.

实现Sr同位素比值（87Sr/86Sr）精确测量的关键环节之一是Sr/基质分离。传统上，这一步是使用Sr-spec树脂完成的，该树脂允许Sr回收消除主要干扰物质（即Ca， Rb, Pb）。虽然这种应用得到了巩固，但文献中很少有关于从多个样品中连续分离Sr的树脂的可能性的细节。为了深化这方面的研究，在本研究中，使用认证和内部标准物质进行了不同的测试，以检查不同工况下的分离性能，并在连续使用周期之间测试HNO3和HCl溶液用于树脂洗涤。这些测试证实，树脂重复使用保证Sr的平均回收率高于95%，最终溶液中干扰物质的存在可以忽略不计，并且对Sr同位素比率没有影响。然而，由于在任何情况下都没有实现定量的Sr回收，如果在同一树脂上处理不同的样品，树脂上Sr残留物的存在可能会限制这种应用。对一批实际样品进行的测试表明，使用HNO3 （0.05 M）温和洗涤，尽管存在残留的交叉污染，但在均匀比例的情况下，样品87Sr/86Sr比率没有发生显着变化。在非均质性增加的情况下，即使在树脂洗涤中使用盐酸也可能是不够的。本研究证明了树脂重复使用的可行性，减少了树脂的消耗、相关成本和实验室工作量，并且在处理样品重复或预期连续样品的87Sr/86Sr比例存在中低差异时，不会影响结果的准确性。

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引用次数: 0

Quantitative results of tocopherols, sterols, triterpene esters, and lipid degradation products in shea kernels using native or quantifier ion intensity corrected analogue standards 定量结果生育酚，甾醇，三萜酯，脂质降解产物在乳木果仁使用原生或定量离子强度校正类似物标准

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-30 DOI: 10.1016/j.talo.2025.100570

Kit Granby , Paula Toma , Peter Christensen , Ella Walbeogo , Yaya Dao , Tore K Ravn , Jan H Christensen , Nikoline J Nielsen

In shea kernels from the Vitellaria Paradoxa tree, lipids make up approximately half of the mass. The lipid portion includes a significant proportion of non-glyceridic lipids, also known as unsaponifiable matter (USM). Shea kernel samples from a controlled study in Burkina Faso, investigating local storage conditions in covered soil pits, were analyzed for USM compounds using GC–MS. The quantification was performed using external calibration curves of native (exact compound) or analogue (chemically similar) standards, and relative intensity corrections of quantifier ions were based on EI mass spectra of standards, samples, and MS libraries. The USM compounds amounted to 2.9 ± 0.4 g/100 g (n = 19) of the shea kernel samples. They included three tocopherols, two Δ⁷-sterols, squalene, and triterpenes, where the triterpenes accounted for an average of 98 % of the total quantified beneficial compounds. The triterpenes included four triterpene alcohols, comprising 1 %, ten triterpene acetates, and eight triterpene cinnamates, comprising 97 % of the quantified unsaponifiable extractables. The concentration of the four predominant tetra- and pentacyclic triterpene cinnamate esters were: α-amyrin 907 ± 110 mg/100 g kernels, lupeol 577 ± 73 mg/100 g, butyrospermol 264 ± 44 mg/100 g, and β-amyrin 130 ± 17 mg/100 g. The triterpene acetates were less abundant; the four dominant were of the triterpenes: α-amyrin 268 ± 38 mg/100 g, lupeol 203 ± 39 mg/100 g, butyrospermol 165 ± 32 mg/100 g, and β-amyrin 68 ± 9 mg/100 g. The quantified USM compounds are known to possess antioxidative, anti-inflammatory, and related health effects. Of particular interest are the tetracyclic and pentacyclic triterpene esters due to their exceptionally high levels in shea butter compared to other oils and fats.

在牛油果核从Vitellaria Paradoxa树，脂质约占一半的质量。脂质部分包括非甘油脂质的显著比例，也被称为不可皂化物质（USM）。在布基纳法索的一项对照研究中，研究了当地覆盖土壤坑的储存条件，使用气相色谱-质谱分析了USM化合物。采用天然（精确化合物）或类似物（化学相似）标准品的外部校准曲线进行定量，定量剂离子的相对强度校正基于标准品、样品和质谱库的EI质谱。在乳木果仁样品中，USM化合物含量为2.9±0.4 g/100 g （n = 19）。它们包括三种生育酚，两种Δ7-sterols，角鲨烯和三萜，其中三萜平均占总量化有益化合物的98%。三萜包括四种三萜醇，含1%，十种三萜乙酸酯和八种三萜肉桂酸酯，含97%的定量不可皂化萃取物。肉桂酸四、五环三萜酯的含量分别为：α-amyrin 907±110 mg/ 100g， luppeol 577±73 mg/ 100g, butyrospermol 264±44 mg/ 100g, β-amyrin 130±17 mg/ 100g。乙酸三萜含量较少；结果表明：α-amyrin（268±38 mg/100 g）、luppeol（203±39 mg/100 g）、butyrosspermol（165±32 mg/100 g）、β-amyrin（68±9 mg/100 g）占主导地位。已知定量USM化合物具有抗氧化，抗炎和相关的健康作用。特别令人感兴趣的是四环和五环三萜酯，因为与其他油和脂肪相比，乳木果油中的含量异常高。

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引用次数: 0

Sensitive detection Cu/Zn SOD mRNA with CRISPR/Cas9 利用CRISPR/Cas9灵敏检测Cu/Zn SOD mRNA

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-29 DOI: 10.1016/j.talo.2025.100568

Geng Qiuyu , Xing Hanxin , Cang Song , Fan Ronghua , Zheng Anqi

Cu/Zn SOD mRNA plays a critical role in protecting cells from oxygen toxicity by regulating the expression of the Cu/Zn SOD enzyme. In this work, we developed a biosensor, termed the Cas9-sgRNA/blocker system, for the analysis of Cu/Zn SOD mRNA. The biosensor was assembled by hybridizing a crRNA with a complementary blocker strand, which was then complexed with the Cas9 nuclease. In the presence of the target mRNA, competitive binding between the blocker strand and the mRNA restored Cas9-sgRNA cleavage activity, enabling enzymatic cleavage of adjacent fluorescent dsDNA reporters. Fluorescence measurements were performed at λex/λem = 488/525 nm, yielding a detection limit of 1.40 nmol L⁻¹. This method demonstrated excellent selectivity for Cu/Zn SOD mRNA, as evidenced by its successful application to the detection of Cu/Zn SOD mRNA in real samples.

Cu/Zn SOD mRNA通过调节Cu/Zn SOD酶的表达，在保护细胞免受氧毒性中起着关键作用。在这项工作中，我们开发了一种生物传感器，称为Cas9-sgRNA/阻断剂系统，用于分析Cu/Zn SOD mRNA。通过将crRNA与互补的阻滞剂链杂交组装生物传感器，然后将其与Cas9核酸酶络合。在目标mRNA存在的情况下，阻断链和mRNA之间的竞争性结合恢复了Cas9-sgRNA的切割活性，使相邻的荧光dsDNA报告基因能够被酶切。荧光测量在λex/λem = 488/525 nm处进行，检测限为1.40 nmol L⁻¹。该方法对Cu/Zn SOD mRNA具有良好的选择性，并成功应用于实际样品中Cu/Zn SOD mRNA的检测。

引用次数: 0

Addition of apricot powder and its extracts on the physicochemical properties and sensory attributes of Reconstituted Tobacco 杏粉及其提取物的添加对再造烟草理化特性和感官特性的影响

IF 3.7 Q1 CHEMISTRY, ANALYTICAL

Talanta Open

Pub Date : 2025-09-28 DOI: 10.1016/j.talo.2025.100567

Gaofeng Dong , Weiyao Hu , Jing Liu , Guiyuan Zhou , Kai Li , Jin Xu , Olivier Berthou , Huifan Li , Yuxin Liu

Reconstituted tobacco (RT), is a product for reducing tar and harm in cigarettes. It is also the main means of resource utilization of tobacco waste. However, RT products are often limited in their wide application due to the traditional monotonic flavor from the poor stability of added flavor agents during processing. It is of considerable interest to investigate flavor diversification of RT. In this study, the effects of ultra-fine apricot powder with different particle sizes and addition rates on the characteristics of RT were investigated. The result shows to optimize the performance of physical and chemical indexes of RT, an 800 mesh is the most appropriate particle size and the powder addition rate should be below 15 %. The uniformity of dry weight improved 26.0 %, color difference (L*, a*, b*) 29.1 %, 23.5, 33.8 % respectively. The color difference (ΔE) between it and the standard sample of natural tobacco leaves optimized by 38.7 %. 14 % apricot powder added in forming pulp and 0.15 % apricot extracts added in coating liquid is the best combination in obtaining RT finished products with apricot characteristic aroma and natural fruity taste. These results reveal a potential method for developing high-quality natural plant flavored RT.

再造烟草（RT）是一种降低卷烟中焦油含量和危害的产品。也是烟草废弃物资源化利用的主要手段。然而，由于在加工过程中添加的风味剂稳定性差，传统的风味单调，制约了RT产品的广泛应用。研究杏粉的风味多样性是研究杏粉风味多样性的重要课题。本研究研究了不同粒径和添加量的超细杏粉对杏粉风味的影响。结果表明，为优化RT的理化指标性能，800目为最佳粒度，粉料添加量应低于15%。干重均匀度提高26.0%，色差（L*、a*、b*）分别提高29.1%、23.5%、33.8%。与天然烟叶标准样品的色差（ΔE）优化为38.7%。成浆中添加14%杏粉，包衣液中添加0.15%杏提取物，可获得具有杏特有香气和天然果味的RT成品。这些结果揭示了一种开发高品质天然植物风味RT的潜在方法。

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引用次数: 0