Pub Date : 2026-01-23DOI: 10.1016/j.trac.2026.118697
João Pinto da Costa , Ana Paço , Andy M. Booth , Jes Vollertsen , Alvise Vianello , Teresa Rocha-Santos
Micro- and nanoplastics are increasingly recognized as ubiquitous environmental contaminants with potential implications for ecological and human health. However, many knowledge gaps remain that inhibit comprehensive analyses of the existing data, and, typically, test materials used differ greatly in morphology, surface chemistry and composition from those occurring in the environment. Herein, a comprehensive, stakeholder-oriented evaluation of current analytical capabilities, data needs, and methodological limitations in the quantification of these materials is presented, drawing on the perspectives and needs of regulatory and monitoring authorities, industry, commercial laboratories, academic researchers, and public health organizations, among others.
We advocate for a multi-methodological framework tailored to matrix complexity and stakeholder objectives. Recommendations include prioritizing method harmonization, investing in high-throughput, cost-effective technologies, and fostering interdisciplinary collaboration to address persistent knowledge gaps. The development of open-access databases and standardized metadata reporting is emphasized as essential for enhancing data comparability and utility across sectors.
{"title":"Stakeholder-centric perspectives on micro- and nanoplastic analysis: needs, gaps, and analytical capabilities","authors":"João Pinto da Costa , Ana Paço , Andy M. Booth , Jes Vollertsen , Alvise Vianello , Teresa Rocha-Santos","doi":"10.1016/j.trac.2026.118697","DOIUrl":"10.1016/j.trac.2026.118697","url":null,"abstract":"<div><div>Micro- and nanoplastics are increasingly recognized as ubiquitous environmental contaminants with potential implications for ecological and human health. However, many knowledge gaps remain that inhibit comprehensive analyses of the existing data, and, typically, test materials used differ greatly in morphology, surface chemistry and composition from those occurring in the environment. Herein, a comprehensive, stakeholder-oriented evaluation of current analytical capabilities, data needs, and methodological limitations in the quantification of these materials is presented, drawing on the perspectives and needs of regulatory and monitoring authorities, industry, commercial laboratories, academic researchers, and public health organizations, among others.</div><div>We advocate for a multi-methodological framework tailored to matrix complexity and stakeholder objectives. Recommendations include prioritizing method harmonization, investing in high-throughput, cost-effective technologies, and fostering interdisciplinary collaboration to address persistent knowledge gaps. The development of open-access databases and standardized metadata reporting is emphasized as essential for enhancing data comparability and utility across sectors.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"197 ","pages":"Article 118697"},"PeriodicalIF":12.0,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-22DOI: 10.1016/j.trac.2026.118691
Matthias Anagho-Mattanovich , Hayden Ashley Paige , Ricardo Hernández Medina , Thomas Moritz
Stable-isotope labeling and tracing are powerful approaches in metabolism research, allowing for the observation of atom movement through the biochemical reaction network of a system. By introducing isotopically labeled substrates, researchers can track metabolite transformations, uncover pathway dynamics, and calculate metabolic fluxes. Unlike traditional metabolomics, isotope tracing provides insights into both static and dynamic aspects of metabolism. Modern analytical and computational methods have made stable-isotope labeling more accurate, replacing older unsafe radiolabeling techniques. However, careful experimental design, biological study, chemical analysis, and data processing are essential to avoid pitfalls and ensure accurate, reliable results in isotope labeling studies.
{"title":"Stable-isotope labeling using mass spectrometry for metabolism research","authors":"Matthias Anagho-Mattanovich , Hayden Ashley Paige , Ricardo Hernández Medina , Thomas Moritz","doi":"10.1016/j.trac.2026.118691","DOIUrl":"10.1016/j.trac.2026.118691","url":null,"abstract":"<div><div>Stable-isotope labeling and tracing are powerful approaches in metabolism research, allowing for the observation of atom movement through the biochemical reaction network of a system. By introducing isotopically labeled substrates, researchers can track metabolite transformations, uncover pathway dynamics, and calculate metabolic fluxes. Unlike traditional metabolomics, isotope tracing provides insights into both static and dynamic aspects of metabolism. Modern analytical and computational methods have made stable-isotope labeling more accurate, replacing older unsafe radiolabeling techniques. However, careful experimental design, biological study, chemical analysis, and data processing are essential to avoid pitfalls and ensure accurate, reliable results in isotope labeling studies.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118691"},"PeriodicalIF":12.0,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The simultaneous detection of multiple biomarkers has become increasingly important for healthcare diagnostics, food quality control, and environmental monitoring. Real-world samples are often complex, containing multiple analytes that may interact, leading to cross-reactivity and complicating reliable detection. In response, significant advances have been made in developing multiplexed analytical platforms that enable accurate, efficient, and early detection of disease markers or contaminants. This review systematically analyzes literature published from 2018 to mid-2025, following PRISMA guidelines, to evaluate strategies, technologies, and applications in multiplexed analyte detection platforms. It critically reviews various approaches, including optical, electrochemical, and molecular methods, all of which aim to detect multiple analytes simultaneously in one or more samples. This study also explores a broad range of applications in which multi-target detection is valuable, including real-time monitoring of environmental contaminants and clinical diagnostic settings, where early disease detection is vital. In conclusion, this work highlights current research directions in multi-analyte detection, its limitations, and potential future advancements.
{"title":"Advancements and strategies in simultaneous multi-target detection for analyte multiplexing","authors":"Zhazira Zhumabekova , Nigara Yunussova , Damira Kanayeva","doi":"10.1016/j.trac.2026.118688","DOIUrl":"10.1016/j.trac.2026.118688","url":null,"abstract":"<div><div>The simultaneous detection of multiple biomarkers has become increasingly important for healthcare diagnostics, food quality control, and environmental monitoring. Real-world samples are often complex, containing multiple analytes that may interact, leading to cross-reactivity and complicating reliable detection. In response, significant advances have been made in developing multiplexed analytical platforms that enable accurate, efficient, and early detection of disease markers or contaminants. This review systematically analyzes literature published from 2018 to mid-2025, following PRISMA guidelines, to evaluate strategies, technologies, and applications in multiplexed analyte detection platforms. It critically reviews various approaches, including optical, electrochemical, and molecular methods, all of which aim to detect multiple analytes simultaneously in one or more samples. This study also explores a broad range of applications in which multi-target detection is valuable, including real-time monitoring of environmental contaminants and clinical diagnostic settings, where early disease detection is vital. In conclusion, this work highlights current research directions in multi-analyte detection, its limitations, and potential future advancements.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118688"},"PeriodicalIF":12.0,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This review article provides a comprehensive overview of the latest advances in the preparation, properties, and sensing performance of waste-derived nanomaterials (NMs). This article is novel and informative in its scope, since: (a) different approaches for the synthesis of NMs are investigated, considering the advantages and disadvantages of each one; (b) the efficiency of waste-derived NMs in optical sensors is evaluated; (c) it is discussed how waste precursor chemistry can dictate NM surface functionalities and resulting optical selectivity and sensitivity; (d) it is reviewed how heteroatom doping can tune the electronic structure, bandgap, defect states, and optical absorption of waste-derived NMs; (e) different optical sensing mechanisms of waste-derived NMs, including fluorescence enhancing/quenching, inner-filter effect, Förster resonance energy transfer, and surface-state modulation, are assessed; and (f) current trends and future bottlenecks in relation to the synthesis and use of waste-derived NMs in optical sensing platforms are illustrated.
{"title":"From waste to worth: A comprehensive review on waste-derived nanomaterials for optical sensing applications","authors":"Elham Madani-Nejad , Ahmad Reza Bagheri , Ardeshir Shokrollahi , Faezeh Shahdost-Fard","doi":"10.1016/j.trac.2026.118694","DOIUrl":"10.1016/j.trac.2026.118694","url":null,"abstract":"<div><div>This review article provides a comprehensive overview of the latest advances in the preparation, properties, and sensing performance of waste-derived nanomaterials (NMs). This article is novel and informative in its scope, since: (a) different approaches for the synthesis of NMs are investigated, considering the advantages and disadvantages of each one; (b) the efficiency of waste-derived NMs in optical sensors is evaluated; (c) it is discussed how waste precursor chemistry can dictate NM surface functionalities and resulting optical selectivity and sensitivity; (d) it is reviewed how heteroatom doping can tune the electronic structure, bandgap, defect states, and optical absorption of waste-derived NMs; (e) different optical sensing mechanisms of waste-derived NMs, including fluorescence enhancing/quenching, inner-filter effect, Förster resonance energy transfer, and surface-state modulation, are assessed; and (f) current trends and future bottlenecks in relation to the synthesis and use of waste-derived NMs in optical sensing platforms are illustrated.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118694"},"PeriodicalIF":12.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-21DOI: 10.1016/j.trac.2026.118690
Camille Richer, Fabrice Alliot, Thomas Thiebault
Wastewater-based epidemiology (WBE) is an increasingly important approach for assessing community exposure to pharmaceuticals, illicit drugs, and biomarkers, yet its reliance on autosamplers limits spatial and temporal coverage due to high cost, maintenance, and infrastructure demands. Passive sampling (PS) offers a power-free, time-integrated alternative for monitoring polar organic contaminants in complex wastewater matrices. This review examines the performance of adsorption- and diffusion-based PS devices, emphasizing how calibration, biofouling, and sorbent-analyte interactions govern quantitative reliability. In situ calibration remains essential for robust uptake estimates, while ion-exchange and mixed-mode sorbents provide enhanced selectivity and broader analyte coverage. Beyond targeted screening, PS enables semi-quantitative, non-targeted analyses through high-resolution mass spectrometry, expanding its analytical scope. Rather than replacing autosamplers, PS should be viewed as a complementary technology capable of improving spatial and temporal resolution in WBE. Ongoing innovations in design, calibration, and data interpretation will shape its integration into future wastewater surveillance frameworks.
{"title":"Passive sampling of chemical targets in wastewater-based epidemiology: Promise, pitfalls and pathway forward","authors":"Camille Richer, Fabrice Alliot, Thomas Thiebault","doi":"10.1016/j.trac.2026.118690","DOIUrl":"10.1016/j.trac.2026.118690","url":null,"abstract":"<div><div>Wastewater-based epidemiology (WBE) is an increasingly important approach for assessing community exposure to pharmaceuticals, illicit drugs, and biomarkers, yet its reliance on autosamplers limits spatial and temporal coverage due to high cost, maintenance, and infrastructure demands. Passive sampling (PS) offers a power-free, time-integrated alternative for monitoring polar organic contaminants in complex wastewater matrices. This review examines the performance of adsorption- and diffusion-based PS devices, emphasizing how calibration, biofouling, and sorbent-analyte interactions govern quantitative reliability. In situ calibration remains essential for robust uptake estimates, while ion-exchange and mixed-mode sorbents provide enhanced selectivity and broader analyte coverage. Beyond targeted screening, PS enables semi-quantitative, non-targeted analyses through high-resolution mass spectrometry, expanding its analytical scope. Rather than replacing autosamplers, PS should be viewed as a complementary technology capable of improving spatial and temporal resolution in WBE. Ongoing innovations in design, calibration, and data interpretation will shape its integration into future wastewater surveillance frameworks.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118690"},"PeriodicalIF":12.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-21DOI: 10.1016/j.trac.2026.118682
Aisha Ilyas , Richard D. Webster
Microplastic pollution has emerged as a significant environmental concern, driving the need for reliable detection methodologies. This review provides a comprehensive overview of the current approaches used for microplastic analysis, including sampling strategies, sample treatment methods, and detection techniques. To ensure consistency and comparability across studies, standardized protocols for sampling, sample treatment, and detection are urgently needed. Sampling techniques vary depending on the environmental matrix, e.g., water, sediment, biota, and air. Sample treatment strategies, including digestion, oxidation, and enzymatic degradation, are essential for removing naturally occurring organic contaminants while preserving microplastic integrity. Density separation is often applied to remove the inorganic part of the matrix, and the final treatment step often involves a volume reduction method such as filtration to concentrate microplastics. However, these processes must be carefully optimized to minimize sample loss, degradation, and contamination from plastic apparatus. The review discusses the application of spectroscopic methods such as Fourier transform infrared (FTIR) spectroscopy and Raman spectroscopy that remain the most widely used techniques, while also describing emerging methods such as electrochemical studies, hyperspectral imaging, and terahertz spectroscopy. Mass spectrometry (MS) coupled approaches such as gas chromatography (GC-MS), pyrolysis GC-MS, liquid chromatography (LC-MS), and inductively coupled plasma (ICP-MS) that enable quantification are also covered in the following review.
{"title":"Methods for the detection and quantification of micro- and nano-plastic particles in the environment","authors":"Aisha Ilyas , Richard D. Webster","doi":"10.1016/j.trac.2026.118682","DOIUrl":"10.1016/j.trac.2026.118682","url":null,"abstract":"<div><div>Microplastic pollution has emerged as a significant environmental concern, driving the need for reliable detection methodologies. This review provides a comprehensive overview of the current approaches used for microplastic analysis, including sampling strategies, sample treatment methods, and detection techniques. To ensure consistency and comparability across studies, standardized protocols for sampling, sample treatment, and detection are urgently needed. Sampling techniques vary depending on the environmental matrix, e.g., water, sediment, biota, and air. Sample treatment strategies, including digestion, oxidation, and enzymatic degradation, are essential for removing naturally occurring organic contaminants while preserving microplastic integrity. Density separation is often applied to remove the inorganic part of the matrix, and the final treatment step often involves a volume reduction method such as filtration to concentrate microplastics. However, these processes must be carefully optimized to minimize sample loss, degradation, and contamination from plastic apparatus. The review discusses the application of spectroscopic methods such as Fourier transform infrared (FTIR) spectroscopy and Raman spectroscopy that remain the most widely used techniques, while also describing emerging methods such as electrochemical studies, hyperspectral imaging, and terahertz spectroscopy. Mass spectrometry (MS) coupled approaches such as gas chromatography (GC-MS), pyrolysis GC-MS, liquid chromatography (LC-MS), and inductively coupled plasma (ICP-MS) that enable quantification are also covered in the following review.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"197 ","pages":"Article 118682"},"PeriodicalIF":12.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-21DOI: 10.1016/j.trac.2026.118675
Jiaxi Han, Zilin Fang, Yu Qi, Na Yang, Jianhua Liu, Weina He
Organelle viscosity is a vital biomechanical parameter closely linked to diverse physiological and pathological processes. Molecular rotors that visualize viscosity via changes in fluorescence intensity or lifetime, offer a promising noninvasive approach for measuring organelle viscosity. This review systematically summarizes the structural classifications of molecular rotors, including BODIPY derivatives, D-A probes, A-D-A/D-A-D probes and cross-conjugated probes, with emphasis on their photophysical mechanisms and structure–property relationships. Furthermore, it details the design strategies for achieving organelle-specific targeting across key subcellular compartments, such as cell membrane, lysosome, nucleus, mitochondria, Golgi apparatus, endoplasmic reticulum, etc. Targeting approaches fall into three categories: incorporating organelle-binding ligands, tuning physicochemical properties for selective permeability, and exploiting environment-triggered activation. Finally, we discuss applications of organelle-targeting molecular rotors in disease diagnosis, intraoperative imaging, and mechanobiological studies. By mapping intracellular mechanical landscapes, these probes bridge molecular biology and cellular biomechanics, opening new avenues in both basic research and clinical diagnostics.
{"title":"Chemical design of organelle-targeting molecular rotors for intracellular viscosity imaging","authors":"Jiaxi Han, Zilin Fang, Yu Qi, Na Yang, Jianhua Liu, Weina He","doi":"10.1016/j.trac.2026.118675","DOIUrl":"10.1016/j.trac.2026.118675","url":null,"abstract":"<div><div>Organelle viscosity is a vital biomechanical parameter closely linked to diverse physiological and pathological processes. Molecular rotors that visualize viscosity <em>via</em> changes in fluorescence intensity or lifetime, offer a promising noninvasive approach for measuring organelle viscosity. This review systematically summarizes the structural classifications of molecular rotors, including BODIPY derivatives, D-A probes, A-D-A/D-A-D probes and cross-conjugated probes, with emphasis on their photophysical mechanisms and structure–property relationships. Furthermore, it details the design strategies for achieving organelle-specific targeting across key subcellular compartments, such as cell membrane, lysosome, nucleus, mitochondria, Golgi apparatus, endoplasmic reticulum, etc. Targeting approaches fall into three categories: incorporating organelle-binding ligands, tuning physicochemical properties for selective permeability, and exploiting environment-triggered activation. Finally, we discuss applications of organelle-targeting molecular rotors in disease diagnosis, intraoperative imaging, and mechanobiological studies. By mapping intracellular mechanical landscapes, these probes bridge molecular biology and cellular biomechanics, opening new avenues in both basic research and clinical diagnostics.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"197 ","pages":"Article 118675"},"PeriodicalIF":12.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-21DOI: 10.1016/j.trac.2026.118692
Sirui Liu , Mengyu Huang , Jin Li , Shulin Wang , Wei Lv , Wanshuang Cao , Bingzhi Li , Xing Zhang
Glycoconjugates are central to cellular recognition, signaling, and adhesion, yet their visualization in living systems remains difficult because of low abundance, structural diversity, and the limited specificity of traditional probes. Metabolic glycan labeling provides a means to introduce bioorthogonal groups into cellular glycans, allowing selective attachment of imaging probes. Recent studies have combined this approach with functional nucleic acid technologies such as aptamers, CRISPR/Cas systems, DNAzymes, and isothermal amplification. These combinations improve signal strength, increase selectivity, and support multiplex or multimodal readouts. The programmability and amplification capability of nucleic acids help address long-standing challenges in sensitivity and molecular recognition. This review summarizes recent progress in nucleic acid-assisted metabolic glycan labeling for visualizing glycoconjugates in live cells and in vivo models, outlines representative analytical formats, and highlights remaining challenges. Future opportunities are discussed with attention to expanding the analytical toolkit for glycobiology and molecular imaging.
{"title":"Nucleic acid-assisted metabolic glycan labeling: A promising strategy for enhanced glycoconjugate imaging in cells and in vivo","authors":"Sirui Liu , Mengyu Huang , Jin Li , Shulin Wang , Wei Lv , Wanshuang Cao , Bingzhi Li , Xing Zhang","doi":"10.1016/j.trac.2026.118692","DOIUrl":"10.1016/j.trac.2026.118692","url":null,"abstract":"<div><div>Glycoconjugates are central to cellular recognition, signaling, and adhesion, yet their visualization in living systems remains difficult because of low abundance, structural diversity, and the limited specificity of traditional probes. Metabolic glycan labeling provides a means to introduce bioorthogonal groups into cellular glycans, allowing selective attachment of imaging probes. Recent studies have combined this approach with functional nucleic acid technologies such as aptamers, CRISPR/Cas systems, DNAzymes, and isothermal amplification. These combinations improve signal strength, increase selectivity, and support multiplex or multimodal readouts. The programmability and amplification capability of nucleic acids help address long-standing challenges in sensitivity and molecular recognition. This review summarizes recent progress in nucleic acid-assisted metabolic glycan labeling for visualizing glycoconjugates in live cells and <em>in vivo</em> models, outlines representative analytical formats, and highlights remaining challenges. Future opportunities are discussed with attention to expanding the analytical toolkit for glycobiology and molecular imaging.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118692"},"PeriodicalIF":12.0,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-20DOI: 10.1016/j.trac.2026.118686
Xiaotian Ma , Yanming Liu , Teresa Kumblathan , Camille Hamula , X. Chris Le , Xing-Fang Li
The global resurgence of mpox (monkeypox) underscores the need for rapid detection of the mpox virus (MPXV). Clinical surveillance underreports cases because of the self-limiting nature of MPXV and social stigma. Wastewater-based surveillance (WBS) offers a complementary tool for population-level monitoring and overcomes individual testing barriers. However, the detection of MPXV in wastewater is hindered by low viral loads, complex sample matrices, and a lack of standardized methods. This review summarizes recent advances and challenges in WBS of MPXV, including sampling, sample treatment, nucleic acid amplification and detection, and data interpretation. We emphasize the efficient recovery of viral components (virions and nucleic acids), removal of inhibitors, and use of appropriate controls for method validation. We describe an analytical framework combining composite sampling, viral release and concentration, nucleic acid extraction, isothermal amplification, and molecular detection. Developing robust, field-deployable methods remains a key priority.
{"title":"Perspectives on wastewater surveillance of mpox: Current methods and future needs","authors":"Xiaotian Ma , Yanming Liu , Teresa Kumblathan , Camille Hamula , X. Chris Le , Xing-Fang Li","doi":"10.1016/j.trac.2026.118686","DOIUrl":"10.1016/j.trac.2026.118686","url":null,"abstract":"<div><div>The global resurgence of mpox (monkeypox) underscores the need for rapid detection of the mpox virus (MPXV). Clinical surveillance underreports cases because of the self-limiting nature of MPXV and social stigma. Wastewater-based surveillance (WBS) offers a complementary tool for population-level monitoring and overcomes individual testing barriers. However, the detection of MPXV in wastewater is hindered by low viral loads, complex sample matrices, and a lack of standardized methods. This review summarizes recent advances and challenges in WBS of MPXV, including sampling, sample treatment, nucleic acid amplification and detection, and data interpretation. We emphasize the efficient recovery of viral components (virions and nucleic acids), removal of inhibitors, and use of appropriate controls for method validation. We describe an analytical framework combining composite sampling, viral release and concentration, nucleic acid extraction, isothermal amplification, and molecular detection. Developing robust, field-deployable methods remains a key priority.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"197 ","pages":"Article 118686"},"PeriodicalIF":12.0,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146076679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-20DOI: 10.1016/j.trac.2026.118687
Ceren Karaman , Mustafa Aydemir , Onur Karaman
Stress hormones including cortisol and catecholamines (epinephrine, norepinephrine, and dopamine) play cruical roles in regulating physiological responses to emotional, physical, and metabolic challenges. Their dynamic secretion patterns characterized by pulsatility, diurnal variation, and rapid concentration changes make timely monitoring essential for accurate assessment of stress-related conditions including adrenal disorders, mental health imbalances, cardiovascular dysfunction, and performance stress in athletes. Conventional diagnostic techniques, while precise, rely on invasive sampling, centralized laboratory infrastructure, and delayed feedback, limiting their utility for point-of-care (PoC) applications. Recent research has shown that electrochemical biosensors can meet the practical requirements of stress hormone analysis, offering both high sensitivity and adaptability for integration into wearable formats using sweat, saliva, or interstitial fluid as sample media. This review critically examines the current landscape of electrochemical biosensor technologies tailored for stress hormone detection, with a focus on sensor architecture, biorecognition strategies, signal amplification techniques, and the role of functional nanomaterials. Emphasis is placed on the integration of these sensors into wearable and PoC platforms, enabling rapid or near-continuous monitoring and supporting remote health assessment. Furthermore, key clinical applications, existing limitations, and the translational potential of these technologies within personalized and digital endocrinology are discussed. While several emerging wearable and microfluidic electrochemical systems demonstrate fast or semi-continuous operation, the majority of reported cortisol and catecholamine biosensors remain single-use or endpoint devices. Accordingly, this review highlights advances that enable rapid, on-demand electrochemical monitoring while outlining future directions toward fully continuous stress sensing.
{"title":"Wired for stress: Advances in electrochemical biosensors for monitoring of cortisol and catecholamines via wearable and point-of-care platforms","authors":"Ceren Karaman , Mustafa Aydemir , Onur Karaman","doi":"10.1016/j.trac.2026.118687","DOIUrl":"10.1016/j.trac.2026.118687","url":null,"abstract":"<div><div>Stress hormones including cortisol and catecholamines (epinephrine, norepinephrine, and dopamine) play cruical roles in regulating physiological responses to emotional, physical, and metabolic challenges. Their dynamic secretion patterns characterized by pulsatility, diurnal variation, and rapid concentration changes make timely monitoring essential for accurate assessment of stress-related conditions including adrenal disorders, mental health imbalances, cardiovascular dysfunction, and performance stress in athletes. Conventional diagnostic techniques, while precise, rely on invasive sampling, centralized laboratory infrastructure, and delayed feedback, limiting their utility for point-of-care (PoC) applications. Recent research has shown that electrochemical biosensors can meet the practical requirements of stress hormone analysis, offering both high sensitivity and adaptability for integration into wearable formats using sweat, saliva, or interstitial fluid as sample media. This review critically examines the current landscape of electrochemical biosensor technologies tailored for stress hormone detection, with a focus on sensor architecture, biorecognition strategies, signal amplification techniques, and the role of functional nanomaterials. Emphasis is placed on the integration of these sensors into wearable and PoC platforms, enabling rapid or near-continuous monitoring and supporting remote health assessment. Furthermore, key clinical applications, existing limitations, and the translational potential of these technologies within personalized and digital endocrinology are discussed. While several emerging wearable and microfluidic electrochemical systems demonstrate fast or semi-continuous operation, the majority of reported cortisol and catecholamine biosensors remain single-use or endpoint devices. Accordingly, this review highlights advances that enable rapid, on-demand electrochemical monitoring while outlining future directions toward fully continuous stress sensing.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118687"},"PeriodicalIF":12.0,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号