Trends in Analytical Chemistry最新文献_第3页

Bioorthogonal chemistry-triggered engineering on cell membrane for precise bioimaging in living organisms 生物正交化学触发的细胞膜工程在生物体内的精确生物成像

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-11-26 DOI: 10.1016/j.trac.2025.118557

Yu Ma , Guowei Liang , Jue Wang, Xianbao Sun, Wenjun Zhan, Gaolin Liang

In living organisms, the cell membrane participates in numerous critical biological events, making it an ideal engineering platform for bioimaging. However, conventional engineering methods risk disrupting its native functions, underscoring the demand for highly specific and biocompatible reactions. Bioorthogonal chemistry has thus emerged as a promising solution due to its unique bioorthogonality and high reaction efficiency. Herein, this review summarizes recent advances in bioorthogonal chemistry-based cell membrane engineering for precise bioimaging in living organisms. First, we emphasize representative bioorthogonal approaches (e.g., bioorthogonal ligation, bioorthogonal activation, and bioorthogonal click-to-release) for the precise delivery and/or in situ activation of imaging probes, along with common bioorthogonal platforms (e.g., saccharide, lipid nanoparticle, peptide, and antibody conjugate). Furthermore, bioimaging applications, including the selective visualization of membrane biomarkers, dissection of transmembrane transport and release, and dynamic monitoring of cell-cell interactions, are briefly introduced. Finally, we discuss the clinical translational potential and challenges in this field.

在生物体中，细胞膜参与了许多关键的生物事件，使其成为理想的生物成像工程平台。然而，传统的工程方法有破坏其天然功能的风险，强调了对高度特异性和生物相容性反应的需求。生物正交化学因其独特的生物正交性和较高的反应效率而成为一种很有前途的解决方案。本文综述了基于生物正交化学的细胞膜工程技术在生物精密成像中的研究进展。首先，我们强调具有代表性的生物正交方法（例如，生物正交连接，生物正交激活和生物正交点击释放）用于成像探针的精确递送和/或原位激活，以及常见的生物正交平台（例如，糖，脂质纳米颗粒，肽和抗体偶联物）。此外，简要介绍了生物成像的应用，包括膜生物标志物的选择性可视化，跨膜运输和释放的解剖，以及细胞-细胞相互作用的动态监测。最后，我们讨论了该领域的临床转化潜力和挑战。

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引用次数: 0

Linking pathogens and antibiotic resistance in microbial communities: Insights from omics and isotopic tracing 微生物群落中病原体和抗生素耐药性的联系：来自组学和同位素追踪的见解

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-11-25 DOI: 10.1016/j.trac.2025.118560

Jean Damascene Harindintwali , Leilei Xiang , Yuhao Fu , Tala Navab-Daneshmand , Xin Jiang , Martin Elsner , Zhongjun Jia , Gerd Dercon , Matthias C. Rillig , James M. Tiedje , Fang Wang

The accelerating emergence of antibiotic-resistant pathogens is driven by widespread antibiotic use and escalating environmental disturbances, with resistance and virulence shaped by both genetic determinants and interspecies interactions. Traditional culture-based methods remain insufficient to capture the ecological and functional dynamics of these processes in situ. High-throughput multi-omics, including metagenomics, metatranscriptomics, metaproteomics, and metabolomics, has revealed remarkable taxonomic and functional diversity of resistance and virulence determinants across ecosystems. Yet, these approaches often fall short in pinpointing the specific taxa actively expressing functional traits within complex communities. Stable isotope probing (SIP) addresses this gap by linking microbial identity with metabolic activity, enabling direct detection of organisms that degrade antibiotics or express resistance genes under native conditions. Here, we synthesize advances in omics and isotopic tracing to uncover ecological and mechanistic bases of resistance and virulence, highlighting new insights into horizontal gene transfer, community-level selective pressures, and real-time microbial responses to antimicrobial compounds.

抗生素耐药病原体的加速出现是由抗生素的广泛使用和不断升级的环境干扰所驱动的，其耐药性和毒力由遗传决定因素和种间相互作用共同决定。传统的基于文化的方法仍然不足以捕捉这些过程的生态和功能动态。高通量多组学，包括宏基因组学、亚转录组学、宏蛋白质组学和代谢组学，揭示了生态系统中抗性和毒力决定因素的显著分类和功能多样性。然而，这些方法在确定复杂群落中积极表达功能特征的特定分类群方面往往存在不足。稳定同位素探测（SIP）通过将微生物特性与代谢活动联系起来，解决了这一空白，从而能够直接检测在自然条件下降解抗生素或表达抗性基因的生物体。在这里，我们综合了组学和同位素追踪的进展，揭示了耐药性和毒力的生态和机制基础，重点介绍了水平基因转移、社区水平选择压力和微生物对抗菌化合物的实时反应的新见解。

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引用次数: 0

Structural DNA nanoprobe for intracellular fluorescence biosensing 用于细胞内荧光生物传感的结构DNA纳米探针

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-11-25 DOI: 10.1016/j.trac.2025.118559

Ziqi Xu , Di Gao , Jun-Jie Zhu

Recent advances in live-cell analysis have underscored the demand for precision biosensing tools capable of resolving molecular events with spatiotemporal fidelity. Structural DNA nanoprobes exploit the programmability of Watson-Crick base pairing to arrange functional elements with single-nucleotide precision, enabling optimized fluorescence readout and multiplexed detection in living cells. Their intrinsic programmability, multifunctional integration and biocompatibility establish them as powerful platforms for dynamic cellular monitoring. However, challenges such as nuclease degradation and inefficient intracellular delivery continue to hinder clinical translation, particularly in intracellular biosensing and therapeutics. This review first outlines the fundamental design principles of structural DNA nanoprobes, then examines recent cell-oriented engineering strategies including enhanced stability, improved uptake and targeting, intracellular functionality, and endosomal escape. It further surveys advances in intracellular biosensing applications over the past five years and discusses persisting limitations in operational precision and biological adaptability, with the aim of guiding the rational design of next-generation nanoprobes.

活细胞分析的最新进展强调了对能够以时空保真度解决分子事件的精密生物传感工具的需求。结构DNA纳米探针利用沃森-克里克碱基配对的可编程性，以单核苷酸精度排列功能元件，从而优化荧光读出和活细胞中的多路检测。其固有的可编程性、多功能集成和生物相容性使其成为动态细胞监测的强大平台。然而，诸如核酸酶降解和细胞内递送效率低下等挑战继续阻碍临床翻译，特别是在细胞内生物传感和治疗方面。本文首先概述了结构DNA纳米探针的基本设计原则，然后研究了最近的细胞导向工程策略，包括增强稳定性，改善摄取和靶向性，细胞内功能和内体逃逸。它进一步回顾了过去五年来细胞内生物传感应用的进展，并讨论了在操作精度和生物适应性方面持续存在的局限性，旨在指导下一代纳米探针的合理设计。

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引用次数: 0

Advances in nanotechnology-based platforms for liquid biopsy: Unlocking precision molecular classification in ovarian cancer 基于纳米技术的液体活检平台的进展：解锁卵巢癌精确分子分类

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-11-25 DOI: 10.1016/j.trac.2025.118558

Qingqing Tan , Yan Zhao , Zhaoyang Wu , Weiheng Kong , Lujuan Xu , Fengli Qu

Ovarian cancer (OC) remains the most lethal gynecological malignancy, primarily due to late-stage diagnosis and molecular heterogeneity that complicate treatment. Precision molecular classification is crucial for improving patient outcomes. Liquid biopsy emerges as a transformative, non-invasive approach for monitoring tumor markers, yet its clinical utility is severely constrained by the exceedingly low abundance of biomarkers. Nanotechnology offers powerful tools to overcome these limitations. This review systematically summarizes the latest advances in nanotechnology-based biosensing platforms designed to enhance the performance of liquid biopsy for the precise molecular classification of OC. We highlight diverse nanoplatforms engineered for superior detection of key circulating biomarkers—including CTCs, ctDNA, cfRNA, and extracellular vesicles—which enable early diagnosis, therapy monitoring, and prognostic evaluation across different OC histotypes. Finally, we discuss current challenges and provide a forward-looking perspective on translating these promising nanotechnologies into clinical practice, advancing precision oncology for OC.

卵巢癌（OC）仍然是最致命的妇科恶性肿瘤，主要是由于晚期诊断和分子异质性使治疗复杂化。精确的分子分类对改善患者预后至关重要。液体活检作为一种变革性的、非侵入性的监测肿瘤标志物的方法出现，但其临床应用受到生物标志物丰度极低的严重限制。纳米技术为克服这些限制提供了强大的工具。本文系统地总结了纳米技术为基础的生物传感平台的最新进展，这些平台旨在提高液体活检的性能，以精确地对OC进行分子分类。我们重点介绍了用于检测关键循环生物标志物（包括ctc、ctDNA、cfRNA和细胞外囊泡）的各种纳米平台，这些纳米平台可以实现不同OC组织类型的早期诊断、治疗监测和预后评估。最后，我们讨论了当前的挑战，并提供了前瞻性的观点，将这些有前途的纳米技术转化为临床实践，推进肿瘤的精确肿瘤学。

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引用次数: 0

Metabolomics in environmental analysis: Applications, advances, and emerging challenges 代谢组学在环境分析中的应用、进展和新出现的挑战

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-11-25 DOI: 10.1016/j.trac.2025.118556

Lin Peng , Hong Yan , Zongwei Cai

The growing complexity and ubiquity of environmental contaminants demands sensitive, mechanistically informative tools for exposure science. Metabolomics, capturing system-wide metabolic perturbations from chemical exposures, offers a transformative platform for linking molecular disruptions to organismal and population-level outcomes. This review highlights recent advances in environmental metabolomics, focusing on three key domains: (1) ecological biomonitoring, enhancing early biological effect detection and causality assessment with effect-directed analysis (EDA); (2) mechanistic toxicology, informing Adverse Outcome Pathway (AOP) and quantitative AOP development by bridging multi-scale models from in vivo and in vitro studies; and (3) environmental epidemiology, driving biomarker discovery and pathway inference via metabolome-wide association studies (MWAS), meet-in-the-middle (MITM) frameworks, and advanced statistical, AI computational approaches. By synthesizing methodologies and innovations, this review positions metabolomics as a cornerstone of next-generation environmental health research, advancing predictive, mechanistic chemical risk assessments aligned with New Approach Methodologies (NAMs) in complex scenarios.

日益复杂和无处不在的环境污染物需要敏感的，机械信息暴露科学工具。代谢组学捕获化学暴露引起的全系统代谢扰动，为将分子破坏与生物体和种群水平的结果联系起来提供了一个变革性的平台。本文综述了环境代谢组学的最新进展，主要集中在三个关键领域：(1)生态生物监测，利用效应导向分析（EDA）加强早期生物效应检测和因果关系评估；(2)机制毒理学，通过连接体内和体外研究的多尺度模型，为不良结果通路（AOP）和定量AOP的发展提供信息；(3)环境流行病学，通过代谢组关联研究（MWAS）、中间相遇（MITM）框架和先进的统计、人工智能计算方法，推动生物标志物的发现和途径推断。通过综合方法和创新，本综述将代谢组学定位为下一代环境健康研究的基石，在复杂情况下推进与新方法方法（NAMs）一致的预测性、机械性化学品风险评估。

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引用次数: 0

Multifunctional microfluidic chips in single-cell analysis: design, integration and omics application 单细胞分析中的多功能微流控芯片：设计、集成和组学应用

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-11-24 DOI: 10.1016/j.trac.2025.118554

Xue Zhang , Fenghua Xu , Yufei Shi , Huiying Zhang , Xin Dong , Yu Bai

Multifunctional integrated microfluidic chips (MCs) have emerged as pivotal platforms for high-resolution biological research. With the rapid development of single cell analysis, integrating the traditional multi-step workflows into all-in-one operation provides improvement of analysis throughput and depth. This review systematically summarizes the design principles, technical innovations, and multifunctional integration strategies of MCs tailored for single-cell studies, with a particular focus on their omics applications. We critically compare planar and three-dimensional MCs, dissecting their merits and limitations in precision, throughput, biocompatibility, and scalability. Subsequently, the multifunctional integration strategy involves single-cell processing, microenvironment reconstruction, and multi-parameter responses. Their breakthroughs have significantly promoted transformative progress in single-cell proteomics, metabolomics, transcriptomics, genomics, and multi-omic integrative analyses. By comprehensively summarizing multifunction-MCs application in single-cell evaluation and prospecting their perspectives, this review aims to accelerate the development of efficient, universal single-cell analytical platforms and facilitate their translation into fundamental biomedical research and clinical practice.

多功能集成微流控芯片（MCs）已成为高分辨率生物学研究的关键平台。随着单细胞分析的快速发展，将传统的多步骤工作流程整合为一体化操作，提高了分析的吞吐量和深度。本文系统总结了为单细胞研究量身定制的MCs的设计原则、技术创新和多功能集成策略，并特别关注了它们的组学应用。我们批判性地比较了平面和三维MCs，剖析了它们在精度、吞吐量、生物相容性和可扩展性方面的优点和局限性。随后，多功能整合策略包括单细胞处理、微环境重建和多参数响应。他们的突破极大地推动了单细胞蛋白质组学、代谢组学、转录组学、基因组学和多组学综合分析的变革进展。本文通过对多功能mcs在单细胞评价中的应用进行综述，并对其发展前景进行展望，以期促进高效、通用的单细胞分析平台的发展，促进多功能mcs在基础生物医学研究和临床应用中的应用。

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引用次数: 0

Inner filter effect-based sensing platforms: Strategies to enhance anti-interference capability and sensitivity for on-site analysis 基于内滤波器效应的传感平台：提高现场分析抗干扰能力和灵敏度的策略

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-11-23 DOI: 10.1016/j.trac.2025.118552

Zhankuo Zhang , Yuanyuan Liu , Yao Lin , Jinyi Zhang , Chengbin Zheng

Growing demand for rapid on-site analysis in environmental monitoring, food safety, and point-of-care testing is hindered by matrix interference and limited sensitivity. Fluorescent inner filter effect (IFE)-based sensing platforms have emerged as a promising solution due to their straightforward design, cost-effectiveness, and compatibility with portable devices. This review systematically explores phase-state engineering strategies to enhance the anti-interference capability and sensitivity of IFE probes. It covers foundational liquid-phase systems, reagent-immobilized solid-liquid interfaces, and gas-solid/liquid confined interfaces. We highlight phase-state engineering as a powerful yet underexplored paradigm that promotes matrix separation and analyte enrichment, thereby significantly enhanced detection performance. The integration of portable devices with IFE sensing systems is also highlighted to address challenges in complex sample matrices. This work provides a strategic roadmap for developing robust, field-deployable IFE sensors, bridging the gap between laboratory analysis and real-world applications to support on-site decision-making in resource-limited environments.

环境监测、食品安全和即时检测领域对快速现场分析的需求日益增长，但矩阵干扰和有限的灵敏度阻碍了这一需求。基于荧光内滤光器效应（IFE）的传感平台由于其简单的设计、成本效益和与便携式设备的兼容性而成为一种很有前途的解决方案。本文系统地探讨了提高IFE探针抗干扰能力和灵敏度的相态工程策略。它涵盖了基本的液相系统，试剂固定化固液界面，气固/液受限界面。我们强调相态工程是一种强大但尚未被充分开发的范例，它促进了基质分离和分析物富集，从而显著提高了检测性能。还强调了便携式设备与IFE传感系统的集成，以解决复杂样品矩阵的挑战。这项工作为开发强大的、可现场部署的IFE传感器提供了战略路线图，弥合了实验室分析和实际应用之间的差距，以支持资源有限环境下的现场决策。

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引用次数: 0

Current trends of on-site analytical methods for organic contaminant determination in environmental waters 环境水体中有机污染物现场分析方法的发展趋势

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-11-22 DOI: 10.1016/j.trac.2025.118553

Yuanyuan Liu , Zhankuo Zhang , Yao Lin , Jinyi Zhang , Chengbin Zheng

Organic pollutants derived from industrial discharges, agricultural runoff, and pharmaceutical excretions are accelerating global water pollution, therefore necessitating rapid methods and portable devices to enable timely environmental monitoring and public health protection. Conventional laboratory techniques, while accurate, suffer from high costs, delayed results, and limited spatiotemporal coverage, hindering real-time risk assessment and early-warning responses. Recent innovations in field-deployable sensors offer a transformative alternative through smartphone-based colorimetry, miniaturized optical devices, electrochemical detection, and microfluidics to achieve on-site analysis with high sensitivity and cost-effectiveness. This review systematically examines advances in rapid field detection for key water quality indicators and specific contaminants, highlighting breakthroughs in miniaturized optical emission spectrometers, screen-printed electrodes, and automated microfluidic chips. The current technologies overcome critical limitations of centralized laboratories by enabling drone-assisted sampling, unmanned shipborne monitoring, and multiplexed analysis, thus capturing dynamic pollution patterns in complex aquatic environments. The significance of this work lies in its potential to standardize portable protocols for real-time contaminant tracking, ultimately supporting watershed management and contamination mitigation. Future efforts should prioritize balancing sensitivity with portability, enhancing anti-interference capabilities, and integrating artificial intelligence for multi-target analysis.

来自工业排放、农业径流和药品排泄的有机污染物正在加速全球水污染，因此需要快速方法和便携式设备，以便及时监测环境和保护公众健康。传统的实验室技术虽然准确，但存在成本高、结果延迟和时空覆盖有限的问题，妨碍了实时风险评估和早期预警反应。现场可部署传感器的最新创新通过基于智能手机的比色法、小型化光学器件、电化学检测和微流体提供了一种变革性的替代方案，以实现高灵敏度和高成本效益的现场分析。本文系统地考察了关键水质指标和特定污染物快速现场检测的进展，重点介绍了小型化光学发射光谱仪、丝网印刷电极和自动化微流控芯片的突破。目前的技术克服了集中式实验室的关键限制，实现了无人机辅助采样、无人船载监测和多路分析，从而捕获了复杂水生环境中的动态污染模式。这项工作的重要意义在于它有可能标准化实时污染物跟踪的便携式协议，最终支持流域管理和污染缓解。未来的工作应优先考虑平衡灵敏度和可移植性，增强抗干扰能力，以及集成多目标分析的人工智能。

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引用次数: 0

Wearable fluorescent sensors for healthcare monitoring: A review of recent advances, challenges and opportunities 用于医疗监测的可穿戴荧光传感器：最新进展、挑战和机遇的综述

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-11-20 DOI: 10.1016/j.trac.2025.118551

Jian Cai, Jing Bai, Chong-Bo Ma, Xilin Bai, Ming Zhou

Wearable sensors, by enabling real-time, continuous monitoring of target analytes, play a vital role in personalized medicine and early health interventions. Wearable fluorescent sensors have emerged as a promising class of portable diagnostic tools, offering high sensitivity, minimal/noninvasive operation, and real-time visual readout capabilities. This review summarizes recent advances in the design, fabrication, and application of wearable fluorescent sensors, providing an in-depth discussion of critical functional components, including fluorescent probes, flexible substrates, and integration strategies. It highlights representative applications in healthcare monitoring. Particular emphasis is placed on the progression from basic fluorescence patches to microfluidic-integrated systems, detection accuracy, signal stability, and user comfort. The review also addresses current limitations, such as sensitivity, specificity, and device integration, outlining future opportunities from probe design to device optimization. By bridging material science, photonic engineering, and wearable technology, wearable fluorescent sensors are expected to play an increasingly pivotal role in next-generation smart sensing platforms.

可穿戴传感器通过实现对目标分析物的实时、连续监测，在个性化医疗和早期健康干预中发挥着至关重要的作用。可穿戴荧光传感器已经成为一种有前途的便携式诊断工具，提供高灵敏度，最小/无创操作和实时视觉读数能力。本文综述了可穿戴式荧光传感器的设计、制造和应用方面的最新进展，并对关键功能部件进行了深入的讨论，包括荧光探针、柔性衬底和集成策略。重点介绍了医疗保健监控中的代表性应用程序。特别强调的是从基本的荧光贴片到微流体集成系统，检测精度，信号稳定性和用户舒适度的进展。该综述还解决了当前的局限性，如灵敏度、特异性和设备集成，概述了从探针设计到设备优化的未来机会。通过连接材料科学、光子工程和可穿戴技术，可穿戴荧光传感器有望在下一代智能传感平台中发挥越来越重要的作用。

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引用次数: 0

Advances in interfacial modulation for enhancing electrochemiluminescence-based biochemical analysis 界面调制增强电化学发光生化分析的研究进展

IF 12 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry

Pub Date : 2025-11-19 DOI: 10.1016/j.trac.2025.118550

Chukai Lin, Yidan Rong, Min Qi, Wenxuan Fu, Bin Su

Electrochemiluminescence (ECL) is a highly sensitive and versatile signal transduction technique widely employed in biochemical analysis. As it is triggered by electrochemical processes at the electrode interface, the properties of the electrode interface are critical to ECL-based biochemical assays, directly determining both ECL efficiency and analytical performance. In recent years, the development of advanced electrode interfaces to enhance the sensitivity, selectivity and stability of ECL assays has received widespread attention. This review provides a comprehensive overview of recent progress in the design and optimization of electrode interfaces for improving ECL assays. Strategies of enhancing the ECL intensity, promoting the biomolecule immobilization efficiency and increasing the resistance to matrix interferences are summarized. The development and application of novel interface materials for low-potential ECL are explored. The review concludes with a perspective on future directions in ECL-based biochemical analysis, highlighting the potential of interface modulation to drive further innovations in the field.

电化学发光是一种高灵敏度、多用途的信号转导技术，广泛应用于生化分析。由于它是由电极界面的电化学过程触发的，因此电极界面的性质对基于ECL的生化分析至关重要，直接决定了ECL的效率和分析性能。近年来，开发先进的电极界面来提高ECL检测的灵敏度、选择性和稳定性受到了广泛的关注。本文综述了用于改进ECL检测的电极界面的设计和优化方面的最新进展。综述了增强ECL强度、提高生物分子固定化效率和增强抗基质干扰能力的策略。探讨了新型低电位ECL界面材料的开发与应用。本文最后展望了基于ecl的生化分析的未来方向，强调了界面调制在推动该领域进一步创新方面的潜力。

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引用次数: 0