Ultrasonics Sonochemistry最新文献_第5页

Ultrasonic-assisted enzymatic extraction, physicochemical properties and prebiotic activities of polysaccharides from Saccharomyces cerevisiae spore wall

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-02-08 DOI: 10.1016/j.ultsonch.2025.107258

Mengqing Yan , Guoyu Liu , Shiwei Liu , Jia Liu , Haizhi Li , Haotian Wang , Yan Zou , Cong Pan , Fang Zhou , Xueying Zeng , Youqiang Yu , Yimin Wu , Shuheng Yang , Shenglin Duan , Peng Yuan

Yeast wall polysaccharides (YWPs) are a bioactive compound found in the vegetative cells of Saccharomyces cerevisiae, contributing to its applications in food and medicine. YWPs have been extensively studied because of their diverse biological activities. Although the yeast spore wall of S. cerevisiae also contains polysaccharides, the biological activities of the components of the spores have received limited attention. In this study, yeast spore wall polysaccharides (YSWPs) were extracted from chs3Δ spores with exposed β-glucan layers via an ultrasonic-assisted enzymatic extraction (UAE) method, and the optimal extraction process was determined through single-factor and orthogonal experiments. The physicochemical properties of YWPs and YSWPs were compared under identical extraction conditions, and the effects of YSWPs on the abundance of the intestinal microflora and short-chain fatty acids (SCFAs) production in vitro were investigated. The results revealed that the optimal UAE process for YSWPs involves an ultrasonic power of 300 W, an ultrasonic duration of 60 min, an enzyme concentration of 1 % (w/w), and an enzymatic hydrolysis time of 4 h. The total sugar content of the extract was found to be 89.20 ± 0.52 %. The primary monosaccharides present in YSWPs are mannose and glucose, which are consistent with those found in the vegetative cell wall polysaccharides. Both YWPs and YSWPs exhibit similar physical properties, however, YSWPs has a smaller particle size, resulting in superior water-holding and oil-holding capacities. In simulations of in vitro colon fermentation, YSWPs can increased the population of beneficial microorganisms in the human intestine while reducing the number of harmful microbial populations. Additionally, it can effectively produced propionate and butyrate. These findings suggest that YSWPs can help maintain the intestinal ecological balance and promote intestinal health.

{"title":"Ultrasonic-assisted enzymatic extraction, physicochemical properties and prebiotic activities of polysaccharides from Saccharomyces cerevisiae spore wall","authors":"Mengqing Yan , Guoyu Liu , Shiwei Liu , Jia Liu , Haizhi Li , Haotian Wang , Yan Zou , Cong Pan , Fang Zhou , Xueying Zeng , Youqiang Yu , Yimin Wu , Shuheng Yang , Shenglin Duan , Peng Yuan","doi":"10.1016/j.ultsonch.2025.107258","DOIUrl":"10.1016/j.ultsonch.2025.107258","url":null,"abstract":"<div><div>Yeast wall polysaccharides (YWPs) are a bioactive compound found in the vegetative cells of <em>Saccharomyces cerevisiae</em>, contributing to its applications in food and medicine. YWPs have been extensively studied because of their diverse biological activities. Although the yeast spore wall of <em>S. cerevisiae</em> also contains polysaccharides, the biological activities of the components of the spores have received limited attention. In this study, yeast spore wall polysaccharides (YSWPs) were extracted from <em>chs</em>3Δ spores with exposed β-glucan layers via an ultrasonic-assisted enzymatic extraction (UAE) method, and the optimal extraction process was determined through single-factor and orthogonal experiments. The physicochemical properties of YWPs and YSWPs were compared under identical extraction conditions, and the effects of YSWPs on the abundance of the intestinal microflora and short-chain fatty acids (SCFAs) production <em>in vitro</em> were investigated. The results revealed that the optimal UAE process for YSWPs involves an ultrasonic power of 300 W, an ultrasonic duration of 60 min, an enzyme concentration of 1 % (w/w), and an enzymatic hydrolysis time of 4 h. The total sugar content of the extract was found to be 89.20 ± 0.52 %. The primary monosaccharides present in YSWPs are mannose and glucose, which are consistent with those found in the vegetative cell wall polysaccharides. Both YWPs and YSWPs exhibit similar physical properties, however, YSWPs has a smaller particle size, resulting in superior water-holding and oil-holding capacities. In simulations of <em>in vitro</em> colon fermentation, YSWPs can increased the population of beneficial microorganisms in the human intestine while reducing the number of harmful microbial populations. Additionally, it can effectively produced propionate and butyrate. These findings suggest that YSWPs can help maintain the intestinal ecological balance and promote intestinal health.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"114 ","pages":"Article 107258"},"PeriodicalIF":8.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlled bottom-up synthesis and characterization of crystalline and amorphous lead(II) coordination polymers: Sonochemical methods, structure–property relationship, and photocatalytic applications

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-02-08 DOI: 10.1016/j.ultsonch.2025.107264

Saeedeh Ebad Sichani , Mohammad Jaafar Soltanianfard , Alison Zamanpour , Payam Hayati , Jan Janczak , Zahed Karimi-Jaberi

The 3D coordination polymers of lead (II) [Pb₄(O)(L)₃(H₂O)]n, where H₂L = C₆H₄(CO₂H)₂ (benzene-1,3-dicarboxylic acid), were synthesized in crystalline (1(a, b)) and amorphous (2(a, b)) phases. Hydrothermal and branch tube methods were used to self-assemble lead (II) from the bottom up to create the crystalline phases. Single-crystal X-ray diffraction (SC-XRD) was used to investigate two crystallized metal–organic frameworks (MOFs) with the same space group and chemical formula (P21/c). Through controlled green sonochemical methods, an ultrasonic bath and a probe homogenizer were used to prepare nano [Pb₄(O)(L)₃(H₂O)]n in the amorphous phase. An extensive study has been conducted on the effect of initial reagent concentration, ultrasonic power, temperature, reaction time, and surfactant presence on the size and morphology of synthesized nanocoordination polymers. Analyses of the synthesized compounds were performed using scanning electron microscopy (SEM), powder X-ray diffraction (PXRD), FT-IR spectroscopy, and CHNS elemental analysis. By using thermal gravimetry (TGA-DTA), the thermal stability of nanostructures was examined and compared with that of crystalline structures. A Hirschfeld surface and a two-dimensional fingerprint diagram were used to analyze intermolecular interactions in crystalline phases. Photocatalytic degradation of methylene blue was investigated using 1a and 2b_2, which represent the synthetic compounds. Calculation of the band gap of synthesized polymers was conducted using the Tauc diagram (based on the DRS results). During optimal conditions of C0 = 0.6 mg L1, pH = 7, and irradiation time = 60 min, the maximum photodegradation efficiencies of methylene blue (MB) were observed for 1a and 2b_2. In terms of degradation efficiency, 1a’s reusability was 73.5 % for the first cycle and 70.6 % for the fifth, while 2b_2′s reusability was 88.2 % for the first cycle and 81.7 % for the fifth. These materials can be used in multiple photocatalytic cycles based on the results obtained.

{"title":"Controlled bottom-up synthesis and characterization of crystalline and amorphous lead(II) coordination polymers: Sonochemical methods, structure–property relationship, and photocatalytic applications","authors":"Saeedeh Ebad Sichani , Mohammad Jaafar Soltanianfard , Alison Zamanpour , Payam Hayati , Jan Janczak , Zahed Karimi-Jaberi","doi":"10.1016/j.ultsonch.2025.107264","DOIUrl":"10.1016/j.ultsonch.2025.107264","url":null,"abstract":"<div><div>The 3D coordination polymers of lead (II) [Pb<sub>4</sub>(O)(L)<sub>3</sub>(H<sub>2</sub>O)]n, where H<sub>2</sub>L = C<sub>6</sub>H<sub>4</sub>(CO<sub>2</sub>H)<sub>2</sub> (benzene-1,3-dicarboxylic acid), were synthesized in crystalline (1(a, b)) and amorphous (2(a, b)) phases. Hydrothermal and branch tube methods were used to self-assemble lead (II) from the bottom up to create the crystalline phases. Single-crystal X-ray diffraction (SC-XRD) was used to investigate two crystallized metal–organic frameworks (MOFs) with the same space group and chemical formula (P21/c). Through controlled green sonochemical methods, an ultrasonic bath and a probe homogenizer were used to prepare nano [Pb<sub>4</sub>(O)(L)<sub>3</sub>(H<sub>2</sub>O)]n in the amorphous phase. An extensive study has been conducted on the effect of initial reagent concentration, ultrasonic power, temperature, reaction time, and surfactant presence on the size and morphology of synthesized nanocoordination polymers. Analyses of the synthesized compounds were performed using scanning electron microscopy (SEM), powder X-ray diffraction (PXRD), FT-IR spectroscopy, and CHNS elemental analysis. By using thermal gravimetry (TGA-DTA), the thermal stability of nanostructures was examined and compared with that of crystalline structures. A Hirschfeld surface and a two-dimensional fingerprint diagram were used to analyze intermolecular interactions in crystalline phases. Photocatalytic degradation of methylene blue was investigated using 1a and 2b_2, which represent the synthetic compounds. Calculation of the band gap of synthesized polymers was conducted using the Tauc diagram (based on the DRS results). During optimal conditions of C0 = 0.6 mg L1, pH = 7, and irradiation time = 60 min, the maximum photodegradation efficiencies of methylene blue (MB) were observed for 1a and 2b_2. In terms of degradation efficiency, 1a’s reusability was 73.5 % for the first cycle and 70.6 % for the fifth, while 2b_2′s reusability was 88.2 % for the first cycle and 81.7 % for the fifth. These materials can be used in multiple photocatalytic cycles based on the results obtained.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"114 ","pages":"Article 107264"},"PeriodicalIF":8.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The oscillations of non-spherical bubbles in liquid

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-02-08 DOI: 10.1016/j.ultsonch.2025.107262

Yaorong Wu, Zhaokang Lei, Rui Liu, Chenghui Wang

In this paper, the interaction between non-spherical bubbles is studied using a high-speed camera, and the effects of the interaction on the temperature within the bubble and the velocity of the surrounding fluid are theoretically investigated. It is found that the mean radius and the mean wall velocity of the middle bubble in three-bubble system are slightly greater than those in two-bubble system when the initial parameters are consistent. The acoustic response of the middle bubble presented a leftward shift of resonance peak and an increase of resonance peaks with increasing sound pressure. Two patterns of interactions were found in the three-bubble system: steady oscillations with slight non-spherical shape deformation, and a strong coupled state that tends to coalesce. In both patterns, the largest bubble should impose more constraints on the middle bubble, and the middle bubble was observed to be ejected towards the largest. The interacting pattern of bubbles depends on many factors, such as bubble spacing, initial radii of bubbles, acoustic frequency and intensity, which also affect the shape deformation of bubbles. Non-spherical shape deformation decreases the internal gas temperature and disturbs the flow field distribution, all of which are close to cavitation activities. Predictions of the three-bubble model are in good agreement with experimental observations, and can be used to explain bubble behaviors in chained multi-bubble systems in inertial cavitation field.

{"title":"The oscillations of non-spherical bubbles in liquid","authors":"Yaorong Wu, Zhaokang Lei, Rui Liu, Chenghui Wang","doi":"10.1016/j.ultsonch.2025.107262","DOIUrl":"10.1016/j.ultsonch.2025.107262","url":null,"abstract":"<div><div>In this paper, the interaction between non-spherical bubbles is studied using a high-speed camera, and the effects of the interaction on the temperature within the bubble and the velocity of the surrounding fluid are theoretically investigated. It is found that the mean radius and the mean wall velocity of the middle bubble in three-bubble system are slightly greater than those in two-bubble system when the initial parameters are consistent. The acoustic response of the middle bubble presented a leftward shift of resonance peak and an increase of resonance peaks with increasing sound pressure. Two patterns of interactions were found in the three-bubble system: steady oscillations with slight non-spherical shape deformation, and a strong coupled state that tends to coalesce. In both patterns, the largest bubble should impose more constraints on the middle bubble, and the middle bubble was observed to be ejected towards the largest. The interacting pattern of bubbles depends on many factors, such as bubble spacing, initial radii of bubbles, acoustic frequency and intensity, which also affect the shape deformation of bubbles. Non-spherical shape deformation decreases the internal gas temperature and disturbs the flow field distribution, all of which are close to cavitation activities. Predictions of the three-bubble model are in good agreement with experimental observations, and can be used to explain bubble behaviors in chained multi-bubble systems in inertial cavitation field.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"114 ","pages":"Article 107262"},"PeriodicalIF":8.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-assisted deep eutectic solvent extraction of flavonol glycosides from Ginkgo biloba: Optimization of efficiency and mechanism

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-02-08 DOI: 10.1016/j.ultsonch.2025.107254

Jiajia Lai , Peng Zhou , Xiangzhou Li , Ying Lu , Yuqing Wang , Han Yuan , Yanhong Yang

Ultrasound-assisted extraction (UAE) is a technique that can enhance the efficiency of the extraction of bioactive ingredients. In previous work, ginkgo flavonol glycosides (GFG) were extracted using a deep eutectic solvent (DES). To further enhance mass transfer efficiency in this work, ultrasound-assisted deep eutectic solvent (UADES) was employed for GFG extraction. The optimal extraction parameters were determined to be the ultrasonic power 320 W, extraction time 63.6 min, and extraction temperature 32.5℃. The extraction yield of GFG reached 5.60 mg/g, surpassing that achieved through DES extraction. Notably, the extraction time was drastically shortened from 11.8 h to 63.6 min. The analysis of changes in micro-morphology, crystalline structure, and cellulose content of the phytoextraction solid residue revealed that the UADES effectively disrupted the crystalline regions of lignocellulose within the cell wall, and the ultrasound enhanced the solubilization of DES to lignocellulose, thus improving the efficiency of the extraction process.

{"title":"Ultrasound-assisted deep eutectic solvent extraction of flavonol glycosides from Ginkgo biloba: Optimization of efficiency and mechanism","authors":"Jiajia Lai , Peng Zhou , Xiangzhou Li , Ying Lu , Yuqing Wang , Han Yuan , Yanhong Yang","doi":"10.1016/j.ultsonch.2025.107254","DOIUrl":"10.1016/j.ultsonch.2025.107254","url":null,"abstract":"<div><div>Ultrasound-assisted extraction (UAE) is a technique that can enhance the efficiency of the extraction of bioactive ingredients. In previous work, ginkgo flavonol glycosides (GFG) were extracted using a deep eutectic solvent (DES). To further enhance mass transfer efficiency in this work, ultrasound-assisted deep eutectic solvent (UADES) was employed for GFG extraction. The optimal extraction parameters were determined to be the ultrasonic power 320 W, extraction time 63.6 min, and extraction temperature 32.5℃. The extraction yield of GFG reached 5.60 mg/g, surpassing that achieved through DES extraction. Notably, the extraction time was drastically shortened from 11.8 h to 63.6 min. The analysis of changes in micro-morphology, crystalline structure, and cellulose content of the phytoextraction solid residue revealed that the UADES effectively disrupted the crystalline regions of lignocellulose within the cell wall, and the ultrasound enhanced the solubilization of DES to lignocellulose, thus improving the efficiency of the extraction process.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"114 ","pages":"Article 107254"},"PeriodicalIF":8.7,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How does the ultrasonic assist CO2 immiscible flooding?

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-02-07 DOI: 10.1016/j.ultsonch.2025.107260

Hengli Wang , Leng Tian , Yanzhong Zhen , Yating Li , Yi Gao , Gaorun Zhong , Kaiqiang Zhang

CO₂ flooding is considered one of the most important methods for reducing CO₂ emissions and increasing oil production from reservoirs. However, the challenges of low recovery rates and poor economic benefits in CO₂ immiscible flooding significantly hinder its widespread application. To address these issues, this paper proposes the use of ultrasonic-assisted CO₂ immiscible flooding to enhance oil recovery (EOR) and analyzes its underlying mechanisms. First, the distribution characteristics of residual oil in CO₂ immiscible flooding were examined using high pressure mercury injection (HPMI), nuclear magnetic resonance (NMR), and microscopic visualization displacement (MVD) experiments. Next, the changes in oil recovery and residual oil distribution after applying ultrasonic-assisted CO₂ immiscible flooding were analyzed. Finally, the mechanism of EOR through ultrasonic-assisted CO₂ immiscible flooding was summarized based on the results of interfacial tension (IFT) tests. The findings indicate that the recovery rate of CO₂ immiscible flooding without ultrasound is 53.33 %, with residual oil primarily distributed in the form of films and flakes within the pores. The ultrasonic assisted CO₂ immiscible flooding with frequency of 28 KHz and power of 200 W was provided by ultrasonic horn, and the recovery rate increased by 62.84 % after 60 min. With ultrasonic assistance, the recovery rate increased to 62.84 %, and the maximum radius of residual oil droplets was reduced from 81.8 μm to 36.5 μm. Additionally, the amount of residual oil in the 0.1 μm to 1 μm range slightly decreased, suggesting that ultrasonic assistance mainly improves the recovery of flake residual oil, with a slight improvement in film residual oil recovery as well. Ultrasound can enlarge the pore and throat radius and reduce the interfacial tension in the CO₂-oil system, allowing more flake residual oil to be displaced. Moreover, the high-frequency vibrations of the ultrasound can reduce the thickness of the oil film, enhancing the recovery of film residual oil. This study supports the further application of ultrasonic-assisted CO₂ immiscible flooding in both scientific research and engineering practice.

{"title":"How does the ultrasonic assist CO2 immiscible flooding?","authors":"Hengli Wang , Leng Tian , Yanzhong Zhen , Yating Li , Yi Gao , Gaorun Zhong , Kaiqiang Zhang","doi":"10.1016/j.ultsonch.2025.107260","DOIUrl":"10.1016/j.ultsonch.2025.107260","url":null,"abstract":"<div><div>CO<sub>2</sub> flooding is considered one of the most important methods for reducing CO<sub>2</sub> emissions and increasing oil production from reservoirs. However, the challenges of low recovery rates and poor economic benefits in CO<sub>2</sub> immiscible flooding significantly hinder its widespread application. To address these issues, this paper proposes the use of ultrasonic-assisted CO<sub>2</sub> immiscible flooding to enhance oil recovery (EOR) and analyzes its underlying mechanisms. First, the distribution characteristics of residual oil in CO<sub>2</sub> immiscible flooding were examined using high pressure mercury injection (HPMI), nuclear magnetic resonance (NMR), and microscopic visualization displacement (MVD) experiments. Next, the changes in oil recovery and residual oil distribution after applying ultrasonic-assisted CO<sub>2</sub> immiscible flooding were analyzed. Finally, the mechanism of EOR through ultrasonic-assisted CO<sub>2</sub> immiscible flooding was summarized based on the results of interfacial tension (IFT) tests. The findings indicate that the recovery rate of CO<sub>2</sub> immiscible flooding without ultrasound is 53.33 %, with residual oil primarily distributed in the form of films and flakes within the pores. The ultrasonic assisted CO<sub>2</sub> immiscible flooding with frequency of 28 KHz and power of 200 W was provided by ultrasonic horn, and the recovery rate increased by 62.84 % after 60 min. With ultrasonic assistance, the recovery rate increased to 62.84 %, and the maximum radius of residual oil droplets was reduced from 81.8 μm to 36.5 μm. Additionally, the amount of residual oil in the 0.1 μm to 1 μm range slightly decreased, suggesting that ultrasonic assistance mainly improves the recovery of flake residual oil, with a slight improvement in film residual oil recovery as well. Ultrasound can enlarge the pore and throat radius and reduce the interfacial tension in the CO<sub>2</sub>-oil system, allowing more flake residual oil to be displaced. Moreover, the high-frequency vibrations of the ultrasound can reduce the thickness of the oil film, enhancing the recovery of film residual oil. This study supports the further application of ultrasonic-assisted CO<sub>2</sub> immiscible flooding in both scientific research and engineering practice.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"114 ","pages":"Article 107260"},"PeriodicalIF":8.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable Ultrasound-Assisted Solid-Phase peptide synthesis (SUS-SPPS): Less Waste, more efficiency

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-02-07 DOI: 10.1016/j.ultsonch.2025.107257

Salvatore Mottola , Alessandra Del Bene , Vincenzo Mazzarella , Roberto Cutolo , Ida Boccino , Francesco Merlino , Sandro Cosconati , Salvatore Di Maro , Anna Messere

The integration of low-frequency ultrasound with Solid-Phase Peptide Synthesis (SPPS) was explored to establish a Sustainable Ultrasound-assisted Solid-Phase Peptide Synthesis (SUS-SPPS) method. This innovative approach significantly reduces solvent consumption, washing steps, time, and reagent usage compared to conventional manual SPPS protocols. The SUS-SPPS method exploits ultrasound at every stage of synthesis and work-up, reducing the process to just two steps. The first step sequentially combines Fmoc-amino acid coupling, capping of unreacted amino groups, and Fmoc deprotection into a single operation, while the second one consists of a single washing procedure. Moreover, we demonstrated that the method is compatible with various resin types, including Rink-amide, Wang, and Cl-Trt resins, and facilitates the efficient synthesis of peptides of varying lengths (up to 20-mers) and compositions, including those traditionally considered “difficult sequences”, with excellent yields and purity. Notably, SUS-SPPS reduces solvent usage per coupling cycle by 83–88%, marking a significant breakthrough in sustainable peptide synthesis.

{"title":"Sustainable Ultrasound-Assisted Solid-Phase peptide synthesis (SUS-SPPS): Less Waste, more efficiency","authors":"Salvatore Mottola , Alessandra Del Bene , Vincenzo Mazzarella , Roberto Cutolo , Ida Boccino , Francesco Merlino , Sandro Cosconati , Salvatore Di Maro , Anna Messere","doi":"10.1016/j.ultsonch.2025.107257","DOIUrl":"10.1016/j.ultsonch.2025.107257","url":null,"abstract":"<div><div>The integration of low-frequency ultrasound with Solid-Phase Peptide Synthesis (SPPS) was explored to establish a Sustainable Ultrasound-assisted Solid-Phase Peptide Synthesis (SUS-SPPS) method. This innovative approach significantly reduces solvent consumption, washing steps, time, and reagent usage compared to conventional manual SPPS protocols. The SUS-SPPS method exploits ultrasound at every stage of synthesis and work-up, reducing the process to just two steps. The first step sequentially combines Fmoc-amino acid coupling, capping of unreacted amino groups, and Fmoc deprotection into a single operation, while the second one consists of a single washing procedure. Moreover, we demonstrated that the method is compatible with various resin types, including Rink-amide, Wang, and Cl-Trt resins, and facilitates the efficient synthesis of peptides of varying lengths (up to 20-mers) and compositions, including those traditionally considered “difficult sequences”, with excellent yields and purity. Notably, SUS-SPPS reduces solvent usage per coupling cycle by 83–88%, marking a significant breakthrough in sustainable peptide synthesis.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"114 ","pages":"Article 107257"},"PeriodicalIF":8.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharides

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-02-07 DOI: 10.1016/j.ultsonch.2025.107259

Mengdie Ren , Yiru Hou , Danli Peng , Hui Li , Xuxiang Zhang , Lei Qiao , XiaoShuang Wang , Yongmei Jiang , FaMing Wu , Gang Wang

This study investigates two extraction methods—ultrasound-assisted extraction (UAE) and compound enzyme extraction (CE)—for the isolation of Lonicera macranthoides polysaccharides (LMP) and evaluates their physicochemical properties, bioactivity, and therapeutic potential. The optimization of extraction conditions was performed using single-factor experiments and response surface methodology. The yield of LMP from UAE was optimized to 8.02 % ± 0.12 %, while the yield from CE was 10.03 % ± 0.32 %. Both polysaccharide extracts were characterized in terms of chemical composition, monosaccharide composition, molecular weight, thermal stability, and functional group analysis. Notably, UAE-LMP exhibited superior antioxidant, hypolipidemic, nitrite scavenging, and anti-tumor activities compared to CE-LMP. The ultrasound-assisted method enhanced the stability and bioactivity of LMP, with improved water- and oil-holding capacities and the integrity of the active groups. Our findings demonstrate that the extraction method significantly influences the structural and functional properties of LMP, suggesting that UAE may be a more effective approach for extracting bioactive polysaccharides with potential applications in medicine and nutrition.

{"title":"Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides polysaccharides","authors":"Mengdie Ren , Yiru Hou , Danli Peng , Hui Li , Xuxiang Zhang , Lei Qiao , XiaoShuang Wang , Yongmei Jiang , FaMing Wu , Gang Wang","doi":"10.1016/j.ultsonch.2025.107259","DOIUrl":"10.1016/j.ultsonch.2025.107259","url":null,"abstract":"<div><div>This study investigates two extraction methods—ultrasound-assisted extraction (UAE) and compound enzyme extraction (CE)—for the isolation of <em>Lonicera macranthoides</em> polysaccharides (LMP) and evaluates their physicochemical properties, bioactivity, and therapeutic potential. The optimization of extraction conditions was performed using single-factor experiments and response surface methodology. The yield of LMP from UAE was optimized to 8.02 % ± 0.12 %, while the yield from CE was 10.03 % ± 0.32 %. Both polysaccharide extracts were characterized in terms of chemical composition, monosaccharide composition, molecular weight, thermal stability, and functional group analysis. Notably, UAE-LMP exhibited superior antioxidant, hypolipidemic, nitrite scavenging, and anti-tumor activities compared to CE-LMP. The ultrasound-assisted method enhanced the stability and bioactivity of LMP, with improved water- and oil-holding capacities and the integrity of the active groups. Our findings demonstrate that the extraction method significantly influences the structural and functional properties of LMP, suggesting that UAE may be a more effective approach for extracting bioactive polysaccharides with potential applications in medicine and nutrition.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"114 ","pages":"Article 107259"},"PeriodicalIF":8.7,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143376518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental study on the collapse behavior of cavitation bubbles under low ambient pressure conditions

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-02-07 DOI: 10.1016/j.ultsonch.2025.107255

Tong Qu, Jing Luo, Weilin Xu, Jie Li, Guihua Fu, Yueqing Ma, Zhuoqi Zhao

Cavitation bubble dynamics at low ambient pressure differ from those at normal pressure, which can affect the efficiency of cavitation applications in industrial, medical fields, etc. Therefore, a low-voltage discharge method was utilized to produce cavitation bubbles in water at low ambient pressures (56 kPa to 96 kPa), and the impact of low ambient pressure on bubble collapse behavior was systematically studied. Experimental results show that as ambient pressure decreases, the pressure difference between the interior and exterior of bubbles, induced by identical energy, changes during their evolution. Consequently, the bubbles exhibit a gradual increase in maximum radius and an extended evolution period within an unbounded environment. Measurements of the shockwave intensity during bubble collapse revealed that under reduced ambient pressures, the peak pressure of shockwaves from bubble initial collapse gradually diminishes. Correspondingly, the proportion of shockwave energy relative to the bubble’s total mechanical energy also decreases progressively. Based on the behavior of bubbles under low ambient pressure conditions described above, it was found that as ambient pressure decreases, the maximum microjet velocity during cavitation bubble collapse in proximity to rigid walls gradually decreases at a constant bubble-wall distance. Furthermore, the peak of the maximum microjet velocity appears at approximately γ ≈ 0.8 across various ambient pressure conditions. These findings provide insights to enhance cavitation applications, including cavitation erosion prevention, medical treatments, and chemical catalysis in high-altitude and low-pressure environments.

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引用次数: 0

Sustainable pectin extraction from Riang husk using ultrasound-assisted extraction with deep eutectic solvents and its potential in antipollution products

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-02-06 DOI: 10.1016/j.ultsonch.2025.107256

Manee Saelee , Hla Myo , Nuntawat Khat-udomkiri

With increasing concerns about air pollution and its adverse effects on health, particularly in Thailand, the demand for antipollution products has risen significantly. Parkia timoriana (DC.) Merr., commonly known as Riang, has emerged as a promising source for developing antipollution products due to its characteristics. This study investigates the use of ultrasound-assisted extraction (UAE) combined with deep eutectic solvents (DESs) as a sustainable and efficient method for optimizing pectin extraction from Riang husks through the evaluation of a central composite design (CCD), and the structural, functional, and rheological characteristics of the extracted pectin. The antioxidant activity and protective effects against PM2.5-induced cellular damage of this method were also evaluated. The condition that exhibited the highest yield were found to be a liquid-to-solid ratio of 40 mL/g, 35 % amplitude (ultrasonic power of 28.11 W), and 60 min of extraction time. The extracted pectin was primarily composed of monosaccharides, including galacturonic acid (53.74 %), arabinose (23.97 %), galactose (12.36 %), and rhamnose (6.81 %). The degree of esterification (DE) was 73.41 %, classifying it as high methoxyl pectin. Functionally, the pectin demonstrated a solubility of 53 %, a water holding capacity of 3.88 g water/g pectin, an oil holding capacity of 3.30 g oil/g pectin, and a swelling capacity of 11.77 mL/g. Rheological analysis showed shear-thinning behavior across all pH gel forms. Furthermore, Riang husk pectin exhibited antioxidant activity, measured at 0.26 ± 0.02 mmol Trolox equivalents/g, and demonstrated cytoprotective effects against hydrogen peroxide-induced oxidative stress. It also attenuated damage caused by PM2.5 in HaCaT cells. The current study highlights UAE combined with DESs as a sustainable and effective method for obtaining high-quality pectin, contributing to the development of antipollution products and supporting sustainability goals.

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引用次数: 0

Effect of triple-frequency sono-germination and soaking treatments on techno-functional characteristics of barley 三频催芽和浸泡处理对大麦技术功能特性的影响

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-02-01 DOI: 10.1016/j.ultsonch.2025.107231

Tabussam Tufail , Huma Bader Ul Ain , Jawad Ashraf , Muhammad Safiullah Virk , Zahoor Ahmed , Mokhtar Dabbour , Tawfiq Alsulami , Suleiman Althawab , Bin Xu

This research aimed to evaluate the effect of triple-frequency ultrasound treatment (TFUT), germination (GE), and traditional soaking (TS) methods on the nutritional and techno-functional properties of two different barley varieties, including ZQ2000 and XMLY22. Both ZQ2000 and XMLY22 varieties exhibited the highest total phenolic content (TPC) with 840.73 ± 23.59 μg of GAE/g DW and 720.33 ± 30.56 μg of GAE/g DW, and total flavonoid content (TFC) with 520.79 ± 23.45 μg of QUE/g DW and 420.84 ± 19.80 μg of QUE/g DW, respectively. Enzyme activities, such as peroxidase (POD) and polyphenol oxidase (PPO), were notably elevated, indicating enhanced defense mechanisms. The study also found increased γ-Aminobutyric Acid (GABA) levels and antidiabetic potential through inhibition of α-amylase and α-glucosidase enzymes. Further, gene expression analysis revealed differential regulation of phenylpropanoid pathway genes, contributing to the bioactive compound enhancement. Strong intermolecular interactions were observed in both ZQ2000 and XMLY22 samples subjected to TFUT, GE, TFUT + GE, and TS, as validated by FTIR and molecular docking analyses. The structural configuration of two barley types, ZQ2000 and XMLY22, was determined using Fourier transform infrared (FTIR) spectroscopy, which indicated an increase in α-helix and β-sheet conformation and a decrease in random coil conformation in samples treated with TFUT + GE. Moreover, SEM observation provides convincing evidence that TFUT + GE improves and speeds up the breakdown of ZQ2000′s internal structures. Conclusively, this study suggests that the combination of ultrasound and germination treatments significantly enhances the functional properties of barley, making it a promising method for creating health-enhancing barley-based products offering potential applications in functional food development.

本研究旨在评价三频超声处理（TFUT）、发芽（GE）和传统浸泡（TS）对ZQ2000和XMLY22两个不同大麦品种营养和技术功能特性的影响。ZQ2000和XMLY22品种的总酚含量最高，分别为840.73±23.59 μg /g DW和720.33±30.56 μg /g DW；总黄酮含量最高，分别为520.79±23.45 μg /g DW和420.84±19.80 μg /g DW。酶活性，如过氧化物酶（POD）和多酚氧化酶（PPO）显著升高，表明防御机制增强。该研究还发现，通过抑制α-淀粉酶和α-葡萄糖苷酶，γ-氨基丁酸（GABA）水平和降糖潜力增加。此外，基因表达分析显示苯丙素途径基因的差异调控，有助于生物活性化合物的增强。FTIR和分子对接分析证实，ZQ2000和XMLY22样品在TFUT、GE、TFUT + GE和TS作用下均观察到强烈的分子间相互作用。利用傅里叶变换红外光谱（FTIR）测定了ZQ2000和XMLY22两种大麦的结构构型，结果表明，经TFUT + GE处理的样品α-螺旋和β-片状构象增加，随机螺旋形构象减少。此外，SEM观察提供了令人信服的证据，证明TFUT + GE改善并加速了ZQ2000内部结构的分解。综上所述，超声波和发芽处理相结合可以显著提高大麦的功能特性，是一种很有前景的方法，可以开发出增强健康的大麦产品，在功能食品开发中具有潜在的应用前景。

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引用次数: 0