Ultrasonics Sonochemistry最新文献_第4页

Multi-dendrite fragmentation dynamics in acoustic flow with oscillating cavitation bubbles revealed in situ by synchrotron X-ray radiography 同步加速器x射线成像原位显示了振荡空化气泡声流中的多枝晶破碎动力学

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-01 DOI: 10.1016/j.ultsonch.2025.107684

S. Wang , J. Kang , Z. Guo , K. Xiang , J. Wang , X. Li , M. Zou , J. Mi

We used synchrotron X-ray radiography to study in-situ and in real-time the fragmentation dynamics of multiple dendritic microstructures in a Ga-40wt.% In alloy under ultrasound. A dedicated U-shaped quartz tube was designed and used as the sample holder to create well-controlled solidification conditions in a relatively large field of view suitable for imaging acoustic flow, cavitation bubbles, and their dynamic interactions with growing multiple dendrites. Without the presence of oscillating cavitation bubbles, the acoustic flow is able to bend multiple dendrites and then fragment them through cyclic fatigue mechanism over tens to hundreds of seconds. The local swirling flow, formed due to geometric constraints or flow obstacles, can trap and encircle multiple dendrites within the swirl, resulting in 3–4 times higher dendrite fragmentation efficiency than the linear streaming flow. The oscillating cavitation bubbles are more energetic and effective in causing mechanical fatigue fragmentation of dendrites, which often occur at the roots of dendrite arms in a time scale of ∼ 10 ms. Dendrite main trunks may also be fragmented by cavitation bubbles in a much longer time scale (i.e., a few seconds). The real-time observations have unambiguously revealed the fragmentation efficiency of multiple dendrites caused by different flow types and cavitation bubbles in the ultrasound processing conditions that are often found in industrial operations.

我们使用同步加速器x射线摄影技术对Ga-40 %中多个枝晶微观结构的断裂动力学进行了原位和实时研究。超声作用下的合金。设计了一个专用的u形石英管作为样品支架，在大视场内创造良好控制的凝固条件，用于成像声波流动、空化气泡及其与生长的多枝晶的动态相互作用。在没有振荡空化气泡存在的情况下，声波流能够弯曲多个枝晶，然后通过循环疲劳机制在数十到数百秒内将它们破碎。由于几何约束或流动障碍而形成的局部旋流，可以在旋流内捕获和包围多个枝晶，使枝晶破碎效率比线性流高3-4倍。振荡空化气泡更能有效地引起枝晶的机械疲劳断裂，这种断裂通常发生在枝晶臂的根部，时间尺度为 ~ 10 ms。在更长的时间尺度（即几秒钟）内，树突主干也可能被空化气泡破碎。实时观测结果明确地揭示了工业操作中常见的超声条件下由不同流动类型和空化气泡引起的各种枝晶破碎机制的相对重要性和效率

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引用次数: 0

A novel strategy for ultrasound-assisted immobilized enzyme hydrolysis for the preparation of yak bone collagen peptides: Process optimization, structure and antioxidant mechanisms 超声辅助固定化酶水解制备牦牛骨胶原肽的新策略：工艺优化、结构及抗氧化机制

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-01 DOI: 10.1016/j.ultsonch.2025.107689

Yinjuan Cao , Zhaoyang Song , Yabin Ma , Yulong Luo , Jianrui Qi

In recent years, food-derived antioxidant peptides have garnered growing interest within the food industry, owing to their remarkable bioavailability and safety profiles. In this study, collagen peptides derived from yak bone (YCP) were prepared using an ultrasound-assisted immobilized enzyme hydrolysis system (US + IP-EH), and a comprehensive investigation was conducted into their structural characteristics, stability, and underlying antioxidant mechanisms. The results revealed optimal extraction conditions as follows: an ultrasonic power of 415 W, an enzymatic hydrolysis temperature of 49 °C, a hydrolysis duration of 8.4 h, an immobilized enzyme dosage of 3.6 %, and hydrolysis degree (DH) reaching 22.36 % in the validation experiment. Notably, the US + IP-EH approach significantly enhanced the yield, structural integrity, and antioxidant capacity of YCP, enabling them to retain robust antioxidant stability even under challenging conditions such as high temperatures, extreme pH (strong acids or alkalis), light exposure, and gastrointestinal digestion. Furthermore, ten novel antioxidant peptides were successfully identified, demonstrating non-allergenic and non-toxic properties. Among these, peptides P1, P4, and P10 exhibited favorable interactions with Keap1 by occupying its primary binding sites, thereby effectively disrupting the Keap1-Nrf2 complex structure. This research provides a solid theoretical and practical foundation for the development of yak bone collagen into natural functional foods and health products.

近年来，食品来源的抗氧化肽由于其显著的生物利用度和安全性，在食品工业中引起了越来越多的兴趣。本研究采用超声辅助固定化酶水解体系（US + IP-EH）制备牦牛骨胶原肽（YCP），并对其结构特征、稳定性及潜在的抗氧化机制进行了全面研究。结果表明，最佳提取条件为：超声功率415 W，酶解温度49℃，酶解时间8.4 h，固定化酶用量3.6%，水解度（DH）达到22.36%。值得注意的是，US + IP-EH方法显著提高了YCP的产率、结构完整性和抗氧化能力，使其即使在高温、极端pH值（强酸或强碱）、光照和胃肠道消化等具有挑战性的条件下也能保持强大的抗氧化稳定性。此外，还成功鉴定了10种新的抗氧化肽，它们具有非致敏性和无毒性。其中，肽P1、P4和P10占据Keap1的主要结合位点，与Keap1表现出良好的相互作用，从而有效地破坏了Keap1- nrf2复合物的结构。本研究为牦牛骨胶原开发为天然功能食品和保健品提供了坚实的理论和实践基础。

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引用次数: 0

Encapsulation of curcumin in groundnut oil-in-water (O/W) nanoemulsions: Experimental analysis and molecular dynamics simulations 花生水包油纳米乳液中姜黄素的包封：实验分析和分子动力学模拟

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-01 DOI: 10.1016/j.ultsonch.2025.107686

Gerard Nathan Mathias , K Vamshi Krishna Goud , Srikanth Divi , Anoop Kishore Vatti , Jitendra Carpenter , Sivakumar Manickam

This study presents a comprehensive experimental and molecular simulation investigation aimed at developing highly stable and concentrated oil-in-water (O/W) nanoemulsions for curcumin encapsulation, using Tween 80 and Span 80 as emulsifiers. Experimentally, the influence of oil phase concentration (10–30 wt%) on key physicochemical parameters, including droplet size, polydispersity index (PDI), encapsulation efficiency, antioxidant activity, morphology, and long-term stability, was systematically investigated. All formulations exhibited excellent kinetic and oxidative stability during one month of storage, showing no significant changes in droplet size, size distribution, creaming index, or morphology. The encapsulation efficiency and antioxidant activity of curcumin remained consistently high and stable throughout the storage period. Complementing the experimental work, molecular dynamics (MD) simulations were conducted to elucidate the interactions of curcumin with fatty acids in the oil phase and with emulsifiers, thereby providing molecular-level insights into the nanoemulsion system’s structural organization and dynamic behavior. MD simulations confirmed strong molecular interactions between curcumin, oil components, and surfactants, validating the complete encapsulation of curcumin within the oil phase. Quantitative analyses of interaction energy, radius of gyration, and end-to-end distance further elucidated the stabilization mechanism and the cluster-formation behavior of curcumin in the emulsified system. Overall, the formulated emulsions demonstrated outstanding stability and bioactive retention, positioning them as highly effective carriers for curcumin delivery. These findings not only highlight the potential of the developed nanoemulsions for food and pharmaceutical applications but also provide critical molecular-level insights into their stabilization mechanisms, laying a strategic foundation for the design of next-generation functional delivery systems.

以Tween 80和Span 80为乳化剂，对姜黄素包封用高稳定、高浓缩的水包油（O/W）纳米乳进行了综合实验和分子模拟研究。实验中，系统研究了油相浓度（10-30 wt%）对微滴粒径、多分散性指数（PDI）、包封效率、抗氧化活性、形态和长期稳定性等关键理化参数的影响。在一个月的储存过程中，所有配方均表现出优异的动力学和氧化稳定性，液滴大小、粒径分布、成乳指数或形貌均无显著变化。姜黄素的包封率和抗氧化活性在贮藏过程中始终保持较高的稳定性。作为实验工作的补充，我们进行了分子动力学（MD）模拟，以阐明姜黄素与油相脂肪酸和乳化剂的相互作用，从而为纳米乳体系的结构组织和动力学行为提供分子水平的见解。MD模拟证实了姜黄素、油组分和表面活性剂之间强烈的分子相互作用，验证了姜黄素在油相中的完全包封。通过对相互作用能、旋转半径和端到端距离的定量分析，进一步阐明了姜黄素在乳化体系中的稳定机理和团簇形成行为。总的来说，配方乳剂表现出出色的稳定性和生物活性保留，将它们定位为姜黄素递送的高效载体。这些发现不仅突出了所开发的纳米乳液在食品和制药应用中的潜力，而且为其稳定机制提供了关键的分子水平见解，为下一代功能递送系统的设计奠定了战略基础。

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引用次数: 0

Special issue on “Ultrasound-assisted separation processes” “超声辅助分离过程”特刊

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-01 DOI: 10.1016/j.ultsonch.2025.107691

Oualid Hamdaoui

引用次数: 0

Synergistic effects of ultrasonication and alkaline treatment on curcumin-induced cross-linking and gelation of casein-soy protein isolate hybrids 超声和碱性处理对姜黄素诱导的酪蛋白-大豆分离蛋白交联和凝胶化的协同作用

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-01 DOI: 10.1016/j.ultsonch.2025.107690

Xinyi Wu , Minghao Zhang , Benu Adhikari , Feng Xue

The development of hybrid protein gels through the partial replacement of animal proteins with plant-based proteins represents a promising strategy for sustainable food production. This study investigated whether ultrasonication-generated free radicals, alone or combined with alkaline treatment, could induce curcumin to act as a cross-linker between casein and soy protein isolate. Gels were prepared under different pretreatments (alkali, ultrasound, or their combination) and characterized using rheology, spectroscopy, solubility assays, and microscopy. Results demonstrated that only ultrasound and combined treatments induced stable gel formation, with the synergistic treatment exhibiting the strongest gel network, highest cross-linking density, and most ordered protein structure. The enhanced gel properties were attributed to the dual effect of radical-mediated and alkali-driven covalent conjugation. This study provides a novel, enzyme-free approach for constructing hybrid protein gels, offering a sustainable strategy to tailor the texture of plant-protein-enriched foods.

通过用植物蛋白部分替代动物蛋白来开发杂交蛋白凝胶代表了可持续粮食生产的一个有前途的战略。本研究考察了超声波产生的自由基单独或联合碱性处理是否能诱导姜黄素作为酪蛋白和大豆分离蛋白之间的交联剂。凝胶在不同的预处理（碱、超声或其组合）下制备，并使用流变学、光谱、溶解度测定和显微镜进行表征。结果表明，只有超声和联合处理才能诱导稳定的凝胶形成，其中协同处理表现出最强的凝胶网络，最高的交联密度和最有序的蛋白质结构。凝胶性能的增强是由于自由基介导和碱驱动的共价偶联的双重作用。该研究为构建杂交蛋白凝胶提供了一种新的无酶方法，为定制富含植物蛋白的食物的质地提供了一种可持续的策略。

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引用次数: 0

Selected Papers from 18th ESS Meeting 第18届EES会议论文选集。

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-01 DOI: 10.1016/j.ultsonch.2025.107640

Tom Van Gerven , Jean-Yves Hihn

引用次数: 0

Impact of ultrasound-assisted germination and varied drying methods on phenolic biosynthesis, antioxidant capacity, and oxidative enzyme activity in selenium-biofortified black rice 超声辅助萌发和不同干燥方式对硒生物强化黑米酚类生物合成、抗氧化能力和氧化酶活性的影响

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-01 DOI: 10.1016/j.ultsonch.2025.107692

Muhammad Tayyab Rashid , Kunlun Liu , Nazish Muzaffar , Basim M. Alohali , Mushtaque Ahmed Jatoi , Hazrat Usman , Rana Muhammad Aadil

Germinated black rice (GBR) is a nutrition-enriched product with growing consumer interest; however, drying is necessary for shelf life, which may compromise its quality. This study evaluated the effect of ultrasound (US) pretreatment combined with hot-air drying (HAD), infrared vacuum drying (IVD), and freeze-drying (FD) on the drying kinetics, phytochemicals, microstructure, and volatile composition of selenium-enriched GBR. Ultrasound significantly reduced drying time by 16.7 % in HAD and 14.8 % in IVD, with the Hii model providing the best fit for the drying kinetics (R²,1). Ultrasound-assisted IVD improved effective moisture diffusivity (8.11 × 10⁻⁷ m²/s) and lowered energy consumption (12.91 kWh/kg). Phytochemical retention was enhanced, with total phenolic content increased by 28–36 % and total flavonoid content by 15–24 % under HAD and IVD, while FD better preserved individual phenolic acids. Anthocyanin retention was highest in US-IVD samples (cyanidin-3-glucoside: 3075.64 µg/g), whereas carotenoids peaked in IVD without ultrasound. SEM confirmed the presence of microchannels and porosity in US-treated grains, supporting improved mass transfer. Antioxidant activity (DPPH: 1.97 mM TE/g; FRAP: 1.90 mM TE/g) was elevated in US-HAD and US-IVD, while GC–MS showed enrichment of alcohols such as 3-heptanol and suppression of aldehydes, including 2-methyl-2-pentenal. Collectively, ultrasound-assisted HAD and IVD improved drying efficiency, phytochemical stability, and aroma quality of GBR.

发芽黑米（GBR）是一种营养丰富的产品，越来越受到消费者的关注；然而，干燥对于保质期是必要的，这可能会损害其质量。研究了超声（US）预处理联合热风干燥（HAD）、红外真空干燥（IVD）和冷冻干燥（FD）对富硒GBR干燥动力学、植物化学成分、微观结构和挥发性成分的影响。超声可显著减少HAD和IVD的干燥时间，分别减少16.7%和14.8%，其中Hii模型最适合干燥动力学（R2,1）。超声辅助IVD提高了有效水分扩散率（8.11 × 10−7 m2/s），降低了能耗（12.91 kWh/kg）。体外培养和体外培养增强了植物化学物质的保存，总酚含量提高了28 - 36%，总黄酮含量提高了15 - 24%，而体外培养能更好地保存单个酚酸。US-IVD样品的花青素保留率最高（花青素-3-葡萄糖苷：3075.64µg/g），而类胡萝卜素在无超声的IVD样品中最高。扫描电镜证实，在us处理的晶粒中存在微通道和孔隙，支持改善的传质。US-HAD和US-IVD的抗氧化活性（DPPH: 1.97 mM TE/g; FRAP: 1.90 mM TE/g）升高，而GC-MS显示3-庚醇等醇类富集，2-甲基-2-戊烯醛等醛类抑制。总之，超声辅助HAD和IVD提高了GBR的干燥效率、植物化学稳定性和香气品质。

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引用次数: 0

Cavitation-enhanced aroma and browning: A multiscale study of star anise (Illicium verum Hook. f.) pretreated by ultrasound and electrolyzed water 空化增强香气和褐变：八角茴香（八角茴香）的多尺度研究。f)超声和电解水预处理

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-01 DOI: 10.1016/j.ultsonch.2025.107693

Silei Zhang , Qingbo Huang , Xiangmin Kuang , Chaofan Guo , Yongli Jiang , Fujie Zhang , Daihong Pan , Ming Du , Sakamon Devahastin , Junjie Yi

Conventional pretreatments of star anise (Illicium verum Hook. f.) often result in uneven color development, long drying duration and substantial aroma losses, hence limiting final product quality and market value. To address these issues, the present study hypothesized that ultrasound-assisted electrolyzed water thawing (UEWT) as pretreatment prior to drying could accelerate drying and enrich flavor of star anise by inducing appropriate structural and biochemical changes. The effects of UEWT were compared with those of deionized water thawing (DWT), electrolyzed water thawing (EWT), ultrasound-assisted deionized water thawing (UDWT), and hot water blanching (HWB), with a focus on drying kinetics, microstructure, relevant enzyme activities, antioxidant activities and volatiles composition of star anise were evaluated. UEWT reduced drying time to 8 h, with the highest drying rate (0.75 kg/(kg·h)), free water content (48 %) and oil-associated NMR signal (23 %). Enhanced cell disruption enhanced moisture migration and volatiles release, while elevated PPO and POD activities intensified desirable coloration (a* = 27.3; ΔE = 26.8); antioxidants retention was nevertheless compromised (phenolics 45.94 mg GAE/g; flavonoids 2.35 mg GAE/g). UEWT also resulted in the highest content of trans-anethole (1100.40 μg/kg) and formation of α-terpineol, both of which are key contributor to characteristic aroma of star anise. These findings demonstrate that UEWT effectively balances drying rate, color formation, and aroma retention, offering a practical pretreatment for quality-oriented drying of spice fruits. Future studies should optimize ultrasonic parameters and evaluate industrial-scale integration for continuous spice processing.

八角茴香的常规预处理。F.)往往导致颜色发展不均匀，干燥时间长，香气损失大，从而限制了最终产品的质量和市场价值。为了解决这些问题，本研究假设超声辅助电解水解冻（UEWT）作为干燥前预处理可以通过诱导适当的结构和生化变化来加速八角茴香的干燥，并丰富八角茴香的风味。将UEWT与去离子水解冻（DWT）、电解水解冻（EWT）、超声辅助去离子水解冻（UDWT）和热水漂烫（HWB）的效果进行比较，重点评价八角茴香的干燥动力学、微观结构、相关酶活性、抗氧化活性和挥发物组成。UEWT将干燥时间缩短至8 h，干燥速率最高（0.75 kg/(kg·h)），游离含水量最高（48%），油相关核磁共振信号最高（23%）。细胞破坏的增强促进了水分迁移和挥发物的释放，而PPO和POD活性的升高增强了理想的着色（a* = 27.3； ΔE = 26.8）；然而，抗氧化剂的保留受到损害（酚类45.94 mg GAE/g；类黄酮2.35 mg GAE/g）。反式茴香醇含量最高（11000.40 μg/kg）， α-松油醇的生成也是形成八角茴香特征香气的关键物质。这些结果表明，UEWT有效地平衡了干燥速率、颜色形成和香气保留，为香料水果的品质导向干燥提供了实用的预处理。未来的研究应优化超声参数并评估香料连续加工的工业规模集成。

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引用次数: 0

Process optimization and antioxidant activity of white peony root components extracted via ultrasound-assisted deep eutectic solvents 超声辅助深共晶溶剂提取白芍根成分的工艺优化及抗氧化活性研究

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-01 DOI: 10.1016/j.ultsonch.2025.107685

Li Xin , Ammara Sohail , Zihan Li , Yan Cheng , Yilu Wang , Li Cui , Hidayat Hussain , Zheng Wang , Wenshuang Zhao , Jinhua Du , Yue Li , Jixiang He , Daijie Wang

The study aimed to extract antioxidant-rich components from white peony root (WPR) using the ultrasound-assisted deep eutectic solvents (UAE-DES) and optimizing them through response surface methodology to enhance their antioxidant potential. Twenty DESs and three conventional solvents were evaluated, and choline bromide-formic acid (DES17) was found best among them for extracting antioxidant components from WPR. The UAE-DES process was optimized using the Box-Behnken response surface method, achieving a maximum yield (18.16 ± 0.67 mg/g) under optimal conditions (41 min, 1:40 g/mL, and 58 % DES/H₂O), with an extraction efficiency 4–13 times higher than traditional techniques. The study also evaluated the significant DES reusability and recovery of antioxidant components using macroporous resins. Additionally, the DES-derived optimized WPR extract demonstrated significant in vitro antioxidant activity. FTIR, ¹H NMR, SEM, and DFT calculations were carried out to better understand chemical component interactions and extraction mechanisms. This study advances green solvent chemistry and ultrasound applications in natural product processing by providing a viable, environmentally friendly method for the quick and effective extraction of important phytochemicals from plant matrices.

本研究旨在利用超声辅助深共晶溶剂（UAE-DES）提取白芍中富含抗氧化成分，并通过响应面法对其进行优化，以提高其抗氧化能力。对20种溶剂和3种常规溶剂进行了评价，其中溴化胆碱甲酸（DES17）最适合提取水韭中抗氧化成分。采用Box-Behnken响应面法对UAE-DES工艺进行优化，在最佳工艺条件（41 min, 1:40 g/mL, 58 % DES/H2O）下，提取率最高（18.16 ± 0.67 mg/g），提取率比传统工艺提高4-13倍。研究还评估了大孔树脂对DES的可重复使用性和抗氧化成分的回收率。此外，des衍生的优化WPR提取物具有显著的体外抗氧化活性。通过FTIR、1H NMR、SEM和DFT计算，更好地了解化学成分的相互作用和萃取机理。本研究通过提供一种可行的、环境友好的方法，快速有效地从植物基质中提取重要的植物化学物质，促进了绿色溶剂化学和超声波在天然产品加工中的应用。

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引用次数: 0

Ultrasonic frequency and intensity dependence in β2-microglobulin amyloid seed detection: Balancing cavitation-induced nucleation, fragmentation, and radical formation 超声频率和强度在β 2 -微球蛋白淀粉样蛋白种子检测中的依赖性：平衡空化诱导的成核、破碎和自由基形成

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-01 DOI: 10.1016/j.ultsonch.2025.107694

Kichitaro Nakajima , Kakeru Hanada , Masatomo So , Keiichi Yamaguchi , Yuji Goto , Hirotsugu Ogi

Amyloid fibrils are aberrant proteinaceous aggregates involved in several intractable diseases called amyloidosis. Detecting fragments of amyloid fibrils, so-called seeds, in human biofluids is a promising approach for their early-stage diagnosis. Ultrasonication of amyloidogenic protein solutions has the potential to enhance the detection sensitivity of amyloid seeds in an accelerated manner through the effects of ultrasonic cavitation. However, the effects of acoustic frequency and intensity on ultrasonic seed detection have not been investigated. In this study, we explore the optimized acoustic conditions for rapid and sensitive amyloid-seed detection and sonochemical mechanisms behind it. Our results show that maximum detection performance is achieved at moderate acoustic intensities with frequencies below 109 kHz, where the balance between ultrasonic enhancement of nucleation and fragmentation pathways of amyloid formation is suitable for the detection of amyloid seeds. Under these conditions, the detection time for amyloid seeds is reduced fivefold while achieving detection sensitivity of 1 pM. We further find that excessively high-intensity ultrasonication is likely to cause fragmentation of protein monomers into smaller peptides at lower frequencies, while that at frequencies above 200 kHz introduces reactive radical species, hindering the ultrasonic seed detection assay. These results highlight the critical role of optimizing acoustic parameters for the application of ultrasonication in the amyloid-seed detection, offering a pathway for rapid diagnostic assays for amyloidosis.

淀粉样原纤维是一种异常的蛋白质聚集体，与几种称为淀粉样变性的顽固性疾病有关。在人体生物体液中检测淀粉样蛋白原纤维片段，即所谓的种子，是一种很有希望的早期诊断方法。超声检测淀粉样蛋白溶液有可能通过超声空化效应加速提高淀粉样蛋白种子的检测灵敏度。然而，声波频率和强度对超声种子检测的影响尚未得到研究。在这项研究中，我们探索了快速灵敏检测淀粉样蛋白种子的最佳声学条件及其背后的声化学机制。我们的研究结果表明，在频率低于109 kHz的中等声强下，超声增强淀粉样蛋白成核和破碎途径之间的平衡适用于淀粉样蛋白种子的检测。在这些条件下，淀粉样蛋白种子的检测时间减少了五倍，同时实现了1 pM的检测灵敏度。我们进一步发现，过高强度的超声波可能导致蛋白质单体在较低频率下分裂成更小的肽，而在200 kHz以上的频率下引入活性自由基，阻碍了超声波种子检测。这些结果突出了优化声学参数对超声在淀粉样蛋白种子检测中的应用的关键作用，为淀粉样变性的快速诊断分析提供了一条途径。

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引用次数: 0