Pub Date : 2025-12-01DOI: 10.1016/j.ultsonch.2025.107685
Li Xin , Ammara Sohail , Zihan Li , Yan Cheng , Yilu Wang , Li Cui , Hidayat Hussain , Zheng Wang , Wenshuang Zhao , Jinhua Du , Yue Li , Jixiang He , Daijie Wang
The study aimed to extract antioxidant-rich components from white peony root (WPR) using the ultrasound-assisted deep eutectic solvents (UAE-DES) and optimizing them through response surface methodology to enhance their antioxidant potential. Twenty DESs and three conventional solvents were evaluated, and choline bromide-formic acid (DES17) was found best among them for extracting antioxidant components from WPR. The UAE-DES process was optimized using the Box-Behnken response surface method, achieving a maximum yield (18.16 ± 0.67 mg/g) under optimal conditions (41 min, 1:40 g/mL, and 58 % DES/H2O), with an extraction efficiency 4–13 times higher than traditional techniques. The study also evaluated the significant DES reusability and recovery of antioxidant components using macroporous resins. Additionally, the DES-derived optimized WPR extract demonstrated significant in vitro antioxidant activity. FTIR, 1H NMR, SEM, and DFT calculations were carried out to better understand chemical component interactions and extraction mechanisms. This study advances green solvent chemistry and ultrasound applications in natural product processing by providing a viable, environmentally friendly method for the quick and effective extraction of important phytochemicals from plant matrices.
{"title":"Process optimization and antioxidant activity of white peony root components extracted via ultrasound-assisted deep eutectic solvents","authors":"Li Xin , Ammara Sohail , Zihan Li , Yan Cheng , Yilu Wang , Li Cui , Hidayat Hussain , Zheng Wang , Wenshuang Zhao , Jinhua Du , Yue Li , Jixiang He , Daijie Wang","doi":"10.1016/j.ultsonch.2025.107685","DOIUrl":"10.1016/j.ultsonch.2025.107685","url":null,"abstract":"<div><div>The study aimed to extract antioxidant-rich components from white peony root (WPR) using the ultrasound-assisted deep eutectic solvents (UAE-DES) and optimizing them through response surface methodology to enhance their antioxidant potential. Twenty DESs and three conventional solvents were evaluated, and choline bromide-formic acid (DES17) was found best among them for extracting antioxidant components from WPR. The UAE-DES process was optimized using the Box-Behnken response surface method, achieving a maximum yield (18.16 ± 0.67 mg/g) under optimal conditions (41 min, 1:40 g/mL, and 58 % DES/H<sub>2</sub>O), with an extraction efficiency 4–13 times higher than traditional techniques. The study also evaluated the significant DES reusability and recovery of antioxidant components using macroporous resins. Additionally, the DES-derived optimized WPR extract demonstrated significant <em>in vitro</em> antioxidant activity. FTIR, <sup>1</sup>H NMR, SEM, and DFT calculations were carried out to better understand chemical component interactions and extraction mechanisms. This study advances green solvent chemistry and ultrasound applications in natural product processing by providing a viable, environmentally friendly method for the quick and effective extraction of important phytochemicals from plant matrices.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107685"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145554431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amyloid fibrils are aberrant proteinaceous aggregates involved in several intractable diseases called amyloidosis. Detecting fragments of amyloid fibrils, so-called seeds, in human biofluids is a promising approach for their early-stage diagnosis. Ultrasonication of amyloidogenic protein solutions has the potential to enhance the detection sensitivity of amyloid seeds in an accelerated manner through the effects of ultrasonic cavitation. However, the effects of acoustic frequency and intensity on ultrasonic seed detection have not been investigated. In this study, we explore the optimized acoustic conditions for rapid and sensitive amyloid-seed detection and sonochemical mechanisms behind it. Our results show that maximum detection performance is achieved at moderate acoustic intensities with frequencies below 109 kHz, where the balance between ultrasonic enhancement of nucleation and fragmentation pathways of amyloid formation is suitable for the detection of amyloid seeds. Under these conditions, the detection time for amyloid seeds is reduced fivefold while achieving detection sensitivity of 1 pM. We further find that excessively high-intensity ultrasonication is likely to cause fragmentation of protein monomers into smaller peptides at lower frequencies, while that at frequencies above 200 kHz introduces reactive radical species, hindering the ultrasonic seed detection assay. These results highlight the critical role of optimizing acoustic parameters for the application of ultrasonication in the amyloid-seed detection, offering a pathway for rapid diagnostic assays for amyloidosis.
{"title":"Ultrasonic frequency and intensity dependence in β2-microglobulin amyloid seed detection: Balancing cavitation-induced nucleation, fragmentation, and radical formation","authors":"Kichitaro Nakajima , Kakeru Hanada , Masatomo So , Keiichi Yamaguchi , Yuji Goto , Hirotsugu Ogi","doi":"10.1016/j.ultsonch.2025.107694","DOIUrl":"10.1016/j.ultsonch.2025.107694","url":null,"abstract":"<div><div>Amyloid fibrils are aberrant proteinaceous aggregates involved in several intractable diseases called amyloidosis. Detecting fragments of amyloid fibrils, so-called seeds, in human biofluids is a promising approach for their early-stage diagnosis. Ultrasonication of amyloidogenic protein solutions has the potential to enhance the detection sensitivity of amyloid seeds in an accelerated manner through the effects of ultrasonic cavitation. However, the effects of acoustic frequency and intensity on ultrasonic seed detection have not been investigated. In this study, we explore the optimized acoustic conditions for rapid and sensitive amyloid-seed detection and sonochemical mechanisms behind it. Our results show that maximum detection performance is achieved at moderate acoustic intensities with frequencies below 109 kHz, where the balance between ultrasonic enhancement of nucleation and fragmentation pathways of amyloid formation is suitable for the detection of amyloid seeds. Under these conditions, the detection time for amyloid seeds is reduced fivefold while achieving detection sensitivity of 1 pM. We further find that excessively high-intensity ultrasonication is likely to cause fragmentation of protein monomers into smaller peptides at lower frequencies, while that at frequencies above 200 kHz introduces reactive radical species, hindering the ultrasonic seed detection assay. These results highlight the critical role of optimizing acoustic parameters for the application of ultrasonication in the amyloid-seed detection, offering a pathway for rapid diagnostic assays for amyloidosis.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107694"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145593250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-01DOI: 10.1016/j.ultsonch.2025.107639
Jin Guo , Jiale He , Wenlin Xu , Zewei Ma , Rongli Li , Wenjie Xia , Xinpeng Bai , Zhaoxian Huang , Yan Tian , Hongjian Zhang
{"title":"Corrigendum to “Ultrasound combined with pH-shifting improved the emulsification and stability of coconut meal protein isolate by modifying physicochemical properties and intermolecular forces”. [Ultrason. Sonochem. 120 (2025) 107436]","authors":"Jin Guo , Jiale He , Wenlin Xu , Zewei Ma , Rongli Li , Wenjie Xia , Xinpeng Bai , Zhaoxian Huang , Yan Tian , Hongjian Zhang","doi":"10.1016/j.ultsonch.2025.107639","DOIUrl":"10.1016/j.ultsonch.2025.107639","url":null,"abstract":"","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107639"},"PeriodicalIF":9.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145434884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-28DOI: 10.1016/j.ultsonch.2025.107700
Chengjiao Wang , Yepeng Yang , Fulun You , Hang Li , Chunju Yang , Weixian Li , Haidong Ju , Yizhou Li , Liang Jiang , Rao Tao
Photocatalytic hydrogen peroxide (H2O2) production has garnered significant research interest for its environmental benefits and sustainability. S-scheme heterostructure incorporating covalent organic frameworks (COFs) has been recognized as an effective strategy for enhancing photocatalytic H2O2 performance. In this study, MgIn2S4/COFs (MIS/sonoTp-TAPB) S-scheme heterojunction were fabricated via a simple, rapid, and eco-friendly sonochemical method. High-frequency ultrasound induces cavitation effects, generating transient localized high temperatures and pressures, intense microjets, and enhanced mass transfer, effectively overcoming the limitations of conventional synthesis. This approach reduces solvent consumption, shortens reaction duration to 2 h, and ensures sufficient interface contact between MIS and sonoTp-TAPB. The as-obtained MIS/sonoTp-TAPB0.05 heterojunction achieved a photocatalytic H2O2 production rate of 1610.54 μmol·g−1·h−1, which was 2.36 and 3.13 times higher than pristine MIS and sonoTp-TAPB, respectively. The S-scheme structure combines the redox capacity of both MIS and sonoTp-TAPB, and maintains a high H2O2 yield in pure water (1198.2 μmol·g−1·h−1). This enhancement is attributed to abundant surface-active sites, broadened optical absorption, and enhanced carrier separation resuting from S-scheme heterojunction between MIS and sonoTp-TAPB.
{"title":"Sonochemical accelerated fabrication of MgIn2S4/COFs S-scheme heterojunction for efficient photocatalytic H2O2 production","authors":"Chengjiao Wang , Yepeng Yang , Fulun You , Hang Li , Chunju Yang , Weixian Li , Haidong Ju , Yizhou Li , Liang Jiang , Rao Tao","doi":"10.1016/j.ultsonch.2025.107700","DOIUrl":"10.1016/j.ultsonch.2025.107700","url":null,"abstract":"<div><div>Photocatalytic hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) production has garnered significant research interest for its environmental benefits and sustainability. S-scheme heterostructure incorporating covalent organic frameworks (COFs) has been recognized as an effective strategy for enhancing photocatalytic H<sub>2</sub>O<sub>2</sub> performance. In this study, MgIn<sub>2</sub>S<sub>4</sub>/COFs (MIS/sonoTp-TAPB) S-scheme heterojunction were fabricated via a simple, rapid, and eco-friendly sonochemical method. High-frequency ultrasound induces cavitation effects, generating transient localized high temperatures and pressures, intense microjets, and enhanced mass transfer, effectively overcoming the limitations of conventional synthesis. This approach reduces solvent consumption, shortens reaction duration to 2 h, and ensures sufficient interface contact between MIS and sonoTp-TAPB. The as-obtained MIS/sonoTp-TAPB<sub>0.05</sub> heterojunction achieved a photocatalytic H<sub>2</sub>O<sub>2</sub> production rate of 1610.54 μmol·g<sup>−1</sup>·h<sup>−1</sup>, which was 2.36 and 3.13 times higher than pristine MIS and sonoTp-TAPB, respectively. The S-scheme structure combines the redox capacity of both MIS and sonoTp-TAPB, and maintains a high H<sub>2</sub>O<sub>2</sub> yield in pure water (1198.2 μmol·g<sup>−1</sup>·h<sup>−1</sup>). This enhancement is attributed to abundant surface-active sites, broadened optical absorption, and enhanced carrier separation resuting from S-scheme heterojunction between MIS and sonoTp-TAPB.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"124 ","pages":"Article 107700"},"PeriodicalIF":9.7,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145611678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-28DOI: 10.1016/j.ultsonch.2025.107695
Prince Nana Amaniampong , Ascel Samba-Louaka
Free-living amoebae (FLA) are significant environmental reservoirs for numerous pathogenic microorganisms. These protists exhibit remarkable resilience, often evading biocides due to their ability to form highly resistant dormant cysts under unfavorable conditions. This study investigated the effects of ultrasound treatment on the viability of these amoeba cysts, using Acanthamoeba castellanii, a prevalent water and soil amoeba, as a model. We found that low-frequency ultrasound treatment significantly impaired the revival of Acanthamoeba cysts. In contrast, high-frequency ultrasound effectively ruptured cyst walls and induced direct amoebal death. Further investigation using tert-Butanol, an OH radical scavenger, partially mitigated the effects induced by high-frequency ultrasound, demonstrating that reactive oxygen species contribute to amoeba damages. Our results highlight ultrasound’s significant potential as an efficient and effective strategy to combat resilient Acanthamoeba cysts.
{"title":"Combating resilient Acanthamoeba castellanii cysts: Ultrasonic frequency-dependent effects on viability and excystment","authors":"Prince Nana Amaniampong , Ascel Samba-Louaka","doi":"10.1016/j.ultsonch.2025.107695","DOIUrl":"10.1016/j.ultsonch.2025.107695","url":null,"abstract":"<div><div>Free-living amoebae (FLA) are significant environmental reservoirs for numerous pathogenic microorganisms. These protists exhibit remarkable resilience, often evading biocides due to their ability to form highly resistant dormant cysts under unfavorable conditions. This study investigated the effects of ultrasound treatment on the viability of these amoeba cysts, using <em>Acanthamoeba castellanii</em>, a prevalent water and soil amoeba, as a model. We found that low-frequency ultrasound treatment significantly impaired the revival of <em>Acanthamoeba</em> cysts. In contrast, high-frequency ultrasound effectively ruptured cyst walls and induced direct amoebal death. Further investigation using <em>tert</em>-Butanol, an OH radical scavenger, partially mitigated the effects induced by high-frequency ultrasound, demonstrating that reactive oxygen species contribute to amoeba damages. Our results highlight ultrasound’s significant potential as an efficient and effective strategy to combat resilient <em>Acanthamoeba</em> cysts.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"124 ","pages":"Article 107695"},"PeriodicalIF":9.7,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145611676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-28DOI: 10.1016/j.ultsonch.2025.107699
Meng Xu , Wangting Zhou , Yilin Li , Muci Wu , Rui Zhang , Jingren He , Chen Liu
Ultrasound (US) is an effective intensified extraction technology for natural plant resources, but detailed microscale investigations into US-induced plant cell disintegration are lacking. This study explored the correlation between US-induced (P = 0–800 W, t = 0–40 min) cell disintegration degree and the extraction efficiency of intracellular components from purple sweet potato, also discussing extraction selectivity and visualizing the cell micro-disintegration process. Cell disintegration degree was estimated via electrical conductivity (cell permeabilization index, Zi) and fractal dimension analysis of damaged cells (cell wall disintegration index, Zd). Extraction efficiency was evaluated by using the extraction indexes of carbohydrate (Zc), protein (Zp), and total phenolic compound (ZTPC). Results indicated that US markedly enhanced cell permeabilization and intracellular components release. For example, at the highest intensity (800 W, 40 min), the enhancement in carbohydrate, protein and TPC contents were approximately 2 to 7 folds, compared to untreated sample (0 W, 40 min). Extraction efficiencies depended on the applied P and t, with the extractability order was carbohydrate > protein > TPC at each power. Different non-linear correlations between Zd with Zi, Zp, Zc, and ZTPC, reflecting the differences in the release behaviors of these water-soluble components after US treatment. Additionally, US favored carbohydrate extraction over protein, but stronger US intensity resulted in lower extraction selectivity. The visual microscale model showed that increasing P elevated cell surface acoustic pressure, cell stress and volume strain. Cell micro-disintegration process would initiate at the side and end faces, then gradually spread to the edges.
{"title":"Correlation between the degree of ultrasound-induced cell disintegration and the extraction efficiency of intracellular compounds from purple sweet potato","authors":"Meng Xu , Wangting Zhou , Yilin Li , Muci Wu , Rui Zhang , Jingren He , Chen Liu","doi":"10.1016/j.ultsonch.2025.107699","DOIUrl":"10.1016/j.ultsonch.2025.107699","url":null,"abstract":"<div><div>Ultrasound (US) is an effective intensified extraction technology for natural plant resources, but detailed microscale investigations into US-induced plant cell disintegration are lacking. This study explored the correlation between US-induced (<em>P</em> = 0–800 W, <em>t</em> = 0–40 min) cell disintegration degree and the extraction efficiency of intracellular components from purple sweet potato, also discussing extraction selectivity and visualizing the cell micro-disintegration process. Cell disintegration degree was estimated via electrical conductivity (cell permeabilization index, <em>Z<sub>i</sub></em>) and fractal dimension analysis of damaged cells (cell wall disintegration index, <em>Z<sub>d</sub></em>). Extraction efficiency was evaluated by using the extraction indexes of carbohydrate (<em>Z<sub>c</sub></em>), protein (<em>Z<sub>p</sub></em>), and total phenolic compound (<em>Z<sub>TPC</sub></em>). Results indicated that US markedly enhanced cell permeabilization and intracellular components release. For example, at the highest intensity (800 W, 40 min), the enhancement in carbohydrate, protein and TPC contents were approximately 2 to 7 folds, compared to untreated sample (0 W, 40 min). Extraction efficiencies depended on the applied <em>P</em> and <em>t</em>, with the extractability order was carbohydrate > protein > TPC at each power. Different non-linear correlations between <em>Z<sub>d</sub></em> with <em>Z<sub>i</sub></em>, <em>Z<sub>p</sub></em>, <em>Z<sub>c</sub></em>, and <em>Z<sub>TPC</sub></em>, reflecting the differences in the release behaviors of these water-soluble components after US treatment. Additionally, US favored carbohydrate extraction over protein, but stronger US intensity resulted in lower extraction selectivity. The visual microscale model showed that increasing <em>P</em> elevated cell surface acoustic pressure, cell stress and volume strain. Cell micro-disintegration process would initiate at the side and end faces, then gradually spread to the edges.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"124 ","pages":"Article 107699"},"PeriodicalIF":9.7,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145613856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study evaluated the effects of ultrasound, enzymatic hydrolysis (using Alcalase, Papain, and α-Chymotrypsin), and their combination on camel milk casein hydrolysates (CMCH). Proximate analysis confirmed high protein content in casein powder, which was reconstituted for hydrolysis. Results showed that ultrasound treatment significantly increased the degree of hydrolysis across all enzyme groups, with α-chymotrypsin combined with ultrasound achieving the highest peptide release (27.7 % at 6 h). pH measurements revealed accelerated acidification in ultrasound-assisted hydrolysis, while SDS-PAGE confirmed extensive protein breakdown. Antioxidant assays (DPPH and ABTS) demonstrated markedly enhanced radical-scavenging activity in ultrasound-assisted hydrolysates, with CMCH-US- A (camel milk casein hydrolysates-ultrasound-assisted-alcalase) and CMCH-US-C (camel milk casein hydrolysates-ultrasound-assisted-α-chymotrypsin) showing the highest activities. FTIR analysis indicated enzyme-specific structural modifications, with alcalase producing the most pronounced secondary structure changes. Overall, ultrasound-assisted enzymatic hydrolysis effectively enhanced peptide release, antioxidant capacity, and structural modification of camel milk proteins, supporting its potential in functional food and nutraceutical applications.
{"title":"Ultrasound-assisted enzymatic modification: An approach to enhance the quality and functionality of camel milk hydrolysates","authors":"Anita Tanwar , Yogesh Kumar , Rajkumar Berwal , Anil Harsh , Swagatika Priyadarsini , Mitul Bumbadiya","doi":"10.1016/j.ultsonch.2025.107698","DOIUrl":"10.1016/j.ultsonch.2025.107698","url":null,"abstract":"<div><div>This study evaluated the effects of ultrasound, enzymatic hydrolysis (using Alcalase, Papain, and α-Chymotrypsin), and their combination on camel milk casein hydrolysates (CMCH). Proximate analysis confirmed high protein content in casein powder, which was reconstituted for hydrolysis. Results showed that ultrasound treatment significantly increased the degree of hydrolysis across all enzyme groups, with α-chymotrypsin combined with ultrasound achieving the highest peptide release (27.7 % at 6 h). pH measurements revealed accelerated acidification in ultrasound-assisted hydrolysis, while SDS-PAGE confirmed extensive protein breakdown. Antioxidant assays (DPPH and ABTS) demonstrated markedly enhanced radical-scavenging activity in ultrasound-assisted hydrolysates, with CMCH-US- A (camel milk casein hydrolysates-ultrasound-assisted-alcalase) and CMCH-US-C (camel milk casein hydrolysates-ultrasound-assisted-α-chymotrypsin) showing the highest activities. FTIR analysis indicated enzyme-specific structural modifications, with alcalase producing the most pronounced secondary structure changes. Overall, ultrasound-assisted enzymatic hydrolysis effectively enhanced peptide release, antioxidant capacity, and structural modification of camel milk proteins, supporting its potential in functional food and nutraceutical applications.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"124 ","pages":"Article 107698"},"PeriodicalIF":9.7,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145609637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-26DOI: 10.1016/j.ultsonch.2025.107696
Naveed Mahmood , Chang Zheng , Xiaoyan Song , Yuyang Huang , Sajad Ali , Zeeshan Munir , Farzaneh Khorsandi , Yang Li , Fraz Ahmad Khan , Hafiz Mutther Javed
Soy protein isolate, a essential component in various food products, can be greatly impacted by the drying process, influencing its quality and functionality. This study evaluates the effect of ultrasound pretreatments (5, 10, and 15 min at 350 W) and different drying techniques, namely, freeze drying (FD), spray drying (SD) and oven drying (OD) on structural and functional properties of soy protein isolate (SPI) powder. Among all drying conditions, maximum peak temperature Td (175.31 °C) was achieved with FD after 15 min of ultrasound pretreatment. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that ultrasound pretreatment significantly affected protein fragmentation in soy protein isolate (SPI), altering its molecular weight and exhibiting distinct bands compared to control (with distinct bands around 48 kDa and 75 kDa range); demonstrating the most prominent effect of ultrasonic treatment, which further enhanced functional properties. Notably, surface hydrophobicity (H0) of SPI was significantly higher after ultrasound pretreatments compared to their control samples, suggesting that this application time effectively enhanced the exposure of hydrophobic groups. The relative crystallinity of FD-control, SD-control and OD-control samples were decreased by 6.83 % for FD, 8.14 % for SD and 5.30 % for OD after 15 min of ultrasound pretreated drying, respectively. Ultrasound pretreatment significantly reduced contents of off-flavor compounds, such as 3,5-Octadien-2-one, Decanal, (E)-2-octenal and 2-Pentylfuran in SPI. Moreover, relative content of 3,5-Octadien-2-one in the major volatile off-flavor compound of FD dried SPI reduced from 2.27 % to 1.00 %, 1.05 %, and 0.96 % at 5-, 10-, and 15-minute ultrasound (350 W) application times, respectively. These findings provided valuable insights into selecting appropriate ultrasound pretreated drying techniques to optimize the quality and performance of SPI in various applications.
{"title":"Effect of ultrasound pretreatment assisted drying methods on structural and thermal properties of soy protein isolate","authors":"Naveed Mahmood , Chang Zheng , Xiaoyan Song , Yuyang Huang , Sajad Ali , Zeeshan Munir , Farzaneh Khorsandi , Yang Li , Fraz Ahmad Khan , Hafiz Mutther Javed","doi":"10.1016/j.ultsonch.2025.107696","DOIUrl":"10.1016/j.ultsonch.2025.107696","url":null,"abstract":"<div><div>Soy protein isolate, a essential component in various food products, can be greatly impacted by the drying process, influencing its quality and functionality. This study evaluates the effect of ultrasound pretreatments (5, 10, and 15 min at 350 W) and different drying techniques, namely, freeze drying (FD), spray drying (SD) and oven drying (OD) on structural and functional properties of soy protein isolate (SPI) powder. Among all drying conditions, maximum peak temperature T<sub>d</sub> (175.31 °C) was achieved with FD after 15 min of ultrasound pretreatment. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that ultrasound pretreatment significantly affected protein fragmentation in soy protein isolate (SPI), altering its molecular weight and exhibiting distinct bands compared to control (with distinct bands around 48 kDa and 75 kDa range); demonstrating the most prominent effect of ultrasonic treatment, which further enhanced functional properties. Notably, surface hydrophobicity (H<sub>0</sub>) of SPI was significantly higher after ultrasound pretreatments compared to their control samples, suggesting that this application time effectively enhanced the exposure of hydrophobic groups. The relative crystallinity of FD-control, SD-control and OD-control samples were decreased by 6.83 % for FD, 8.14 % for SD and 5.30 % for OD after 15 min of ultrasound pretreated drying, respectively. Ultrasound pretreatment significantly reduced contents of off-flavor compounds, such as 3,5-Octadien-2-one, Decanal, (E)-2-octenal and 2-Pentylfuran in SPI. Moreover, relative content of 3,5-Octadien-2-one in the major volatile off-flavor compound of FD dried SPI reduced from 2.27 % to 1.00 %, 1.05 %, and 0.96 % at 5-, 10-, and 15-minute ultrasound (350 W) application times, respectively. These findings provided valuable insights into selecting appropriate ultrasound pretreated drying techniques to optimize the quality and performance of SPI in various applications.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"124 ","pages":"Article 107696"},"PeriodicalIF":9.7,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145609635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-19DOI: 10.1016/j.ultsonch.2025.107688
Andraž Zupanc , Jernej Ortar , David Pahovnik , Nataša Zabukovec Logar , Blaž Stres , Alenka Šmid , Mojca Zupanc , Martin Petkovšek , Ema Žagar
Low molecular weight chitosan is highly desired for its improved solubility and biological activity compared to high molecular weight chitosan; however, effective, mild and environmentally friendly degradation methods remain a challenge. In this study, we used hydrodynamic cavitation generated by orifice plate constrictions in combination with H2O2 as an oxidizing agent to degrade chitosan to low molecular weight species under mild reaction conditions (room temperature, short reaction time). We systematically investigated parameters such as cavitation intensity, H2O2 concentration, sample preparation, and medium pH that influence the efficiency of chitosan degradation. At 20 °C and after 48 min, corresponding to 800 cavitation passes, the weight-average molecular weight of chitosan decreased from 710 to 20 kDa, with the molecular weight distribution remaining largely unchanged. The degradation performance of cavitation combined with H2O2 clearly exceeded that observed when chitosan was treated with the oxidizing agent alone or with cavitation alone at the same temperature and treatment time (resulting in 390 and 510 kDa, respectively). The degraded chitosan retains the backbone of the original chitosan, while oxidative cleavage of the glycosidic bonds by •OH leads to the formation of chromophore groups, probably located at the chain ends, but their concentration is below the detection limit of FTIR and NMR. The crystallinity and thermal properties of degraded chitosan are largely comparable to those of the original chitosan. The disclosed method provides an environmentally friendly and sustainable alternative to other degradation processes for producing low molecular weight chitosan.
{"title":"Preparation of low molecular weight chitosan through hydroxyl radicals generated via hydrodynamic cavitation","authors":"Andraž Zupanc , Jernej Ortar , David Pahovnik , Nataša Zabukovec Logar , Blaž Stres , Alenka Šmid , Mojca Zupanc , Martin Petkovšek , Ema Žagar","doi":"10.1016/j.ultsonch.2025.107688","DOIUrl":"10.1016/j.ultsonch.2025.107688","url":null,"abstract":"<div><div>Low molecular weight chitosan is highly desired for its improved solubility and biological activity compared to high molecular weight chitosan; however, effective, mild and environmentally friendly degradation methods remain a challenge. In this study, we used hydrodynamic cavitation generated by orifice plate constrictions in combination with H<sub>2</sub>O<sub>2</sub> as an oxidizing agent to degrade chitosan to low molecular weight species under mild reaction conditions (room temperature, short reaction time). We systematically investigated parameters such as cavitation intensity, H<sub>2</sub>O<sub>2</sub> concentration, sample preparation, and medium pH that influence the efficiency of chitosan degradation. At 20 °C and after 48 min, corresponding to 800 cavitation passes, the weight-average molecular weight of chitosan decreased from 710 to 20 kDa, with the molecular weight distribution remaining largely unchanged. The degradation performance of cavitation combined with H<sub>2</sub>O<sub>2</sub> clearly exceeded that observed when chitosan was treated with the oxidizing agent alone or with cavitation alone at the same temperature and treatment time (resulting in 390 and 510 kDa, respectively). The degraded chitosan retains the backbone of the original chitosan, while oxidative cleavage of the glycosidic bonds by <sup>•</sup>OH leads to the formation of chromophore groups, probably located at the chain ends, but their concentration is below the detection limit of FTIR and NMR. The crystallinity and thermal properties of degraded chitosan are largely comparable to those of the original chitosan. The disclosed method provides an environmentally friendly and sustainable alternative to other degradation processes for producing low molecular weight chitosan.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107688"},"PeriodicalIF":9.7,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145560303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-18DOI: 10.1016/j.ultsonch.2025.107687
Chaoya Shi , Bingbing Liu , Zeyi Xiao , Yanfang Huang , Hu Sun , Guihong Han
Tungsten-molybdenum (W-Mo) alloy are critical materials and widely used in aerospace and defense industries. During the manufacture, mechanical processing, and scrapping of W-Mo alloy products, a substantial quantity of waste W-Mo alloy is generated, which holds considerable importance for sustainable critical metals recycling and environmental protection. Hydrometallurgy treatment is demonstrated to be effective to address the multi-component metallic solid waste. However, the alloyed structure and strong chemical inertness of waste W-Mo alloys give rise to low recovery efficiency in conventional leaching processes. This study proposes an integrated ultrasound acid leaching − alkali leaching − solvent extraction process for the W and Mo separation from waste W-Mo alloy. First, the ionic form differences of W and Mo in acidic and alkaline media were investigated to lay the leaching and separation foundations. Thermodynamic calculation indicates that HNO3 can realize the dissolution of W and Mo, while W will hydrolyze to form oxide precipitates, realizing the pre-separation of W and Mo. However, results demonstrate that the dense alloy structure and solid product layer give rise to the poor extraction efficiency in conventional process. The ultrasound-assisted leaching can shorten the leaching time from conventional 4 h to 3 h. It is found that about 97.44 % W and 50.07 % Mo are retained in the residue throughout the acid leaching stage, and a relatively pure Mo leaching solution with 17.51 g/L and 0.32 g/L W was obtained. After that, the acid leaching residue was conducted to alkali leaching to transfer all the W and Mo into the solution as the form of ionic states. Eventually, four-stage N1923 solvent extraction delivers thorough W-Mo separation, and the W and Mo concentrations in raffinate are 0.02 g/L and 20.39 g/L, respectively. Electrochemical analysis and material characterization techniques were employed to highlight the ultrasound-assisted intensification mechanisms. Ultrasound destroys the passivation layer by generating cavitation effects, improving the solid–liquid contact interface and optimizing reaction kinetics. The stepwise separation mechanisms and element migration of W and Mo from the scrap W-Mo alloy during the acid leaching, alkali leaching, and solvent extraction were also discussed. This study proposes an integrated strategy for the efficient separation and recovery of W and Mo from scrap W-Mo alloy.
{"title":"W and Mo separation from waste W-Mo alloy by an integrated ultrasound acid leaching − alkali leaching – Solvent extraction process","authors":"Chaoya Shi , Bingbing Liu , Zeyi Xiao , Yanfang Huang , Hu Sun , Guihong Han","doi":"10.1016/j.ultsonch.2025.107687","DOIUrl":"10.1016/j.ultsonch.2025.107687","url":null,"abstract":"<div><div>Tungsten-molybdenum (W-Mo) alloy are critical materials and widely used in aerospace and defense industries. During the manufacture, mechanical processing, and scrapping of W-Mo alloy products, a substantial quantity of waste W-Mo alloy is generated, which holds considerable importance for sustainable critical metals recycling and environmental protection. Hydrometallurgy treatment is demonstrated to be effective to address the multi-component metallic solid waste. However, the alloyed structure and strong chemical inertness of waste W-Mo alloys give rise to low recovery efficiency in conventional leaching processes. This study proposes an integrated ultrasound acid leaching − alkali leaching − solvent extraction process for the W and Mo separation from waste W-Mo alloy. First, the ionic form differences of W and Mo in acidic and alkaline media were investigated to lay the leaching and separation foundations. Thermodynamic calculation indicates that HNO<sub>3</sub> can realize the dissolution of W and Mo, while W will hydrolyze to form oxide precipitates, realizing the pre-separation of W and Mo. However, results demonstrate that the dense alloy structure and solid product layer give rise to the poor extraction efficiency in conventional process. The ultrasound-assisted leaching can shorten the leaching time from conventional 4 h to 3 h. It is found that about 97.44 % W and 50.07 % Mo are retained in the residue throughout the acid leaching stage, and a relatively pure Mo leaching solution with 17.51 g/L and 0.32 g/L W was obtained. After that, the acid leaching residue was conducted to alkali leaching to transfer all the W and Mo into the solution as the form of ionic states. Eventually, four-stage N1923 solvent extraction delivers thorough W-Mo separation, and the W and Mo concentrations in raffinate are 0.02 g/L and 20.39 g/L, respectively. Electrochemical analysis and material characterization techniques were employed to highlight the ultrasound-assisted intensification mechanisms. Ultrasound destroys the passivation layer by generating cavitation effects, improving the solid–liquid contact interface and optimizing reaction kinetics. The stepwise separation mechanisms and element migration of W and Mo from the scrap W-Mo alloy during the acid leaching, alkali leaching, and solvent extraction were also discussed. This study proposes an integrated strategy for the efficient separation and recovery of W and Mo from scrap W-Mo alloy.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107687"},"PeriodicalIF":9.7,"publicationDate":"2025-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145554427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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