Ultrasonics Sonochemistry最新文献_第6页

Application potential of Lysimachia christinae Hance polysaccharides in kidney stone prevention/treatment: a multidimensional comparison between ultrasonic-assisted extraction and hot water extraction 枸杞子多糖在防治肾结石中的应用潜力：超声辅助提取与热水提取的多维比较

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-13 DOI: 10.1016/j.ultsonch.2025.107720

Jinfu Wang , Jiaxing Lin , Jiayue Wu , Honghong Pan , Jiaxuan Liao , Qinghui Lu , Ruo Wang , Jinfeng Wu , Jiawen Wang

Lysimachia christinae Hance (LCH), a traditional Chinese herbal medicine, possesses the effects of “clearing heat and promoting diuresis, relieving stranguria and expelling stones, and detoxifying and protecting the kidneys”. This study for the first time developed an efficient, green, and economical ultrasonic-assisted extraction (UAE) process for extracting LCH polysaccharides (LCHP-UAE). Meanwhile, it systematically investigated the mechanisms of action of UAE and hot water extraction (HWE) technologies for extracting LCH polysaccharides. Finally, it comparatively analyzed the differences in physicochemical properties, functional characteristics, and bioactivities between LCHP-UAE and LCH polysaccharides obtained by HWE (LCHP-HWE). It was found that under the optimal operating conditions of UAE obtained by response surface methodology optimization, the extraction yield of LCHP-UAE reached 8.25 ± 0.13 %. Compared with HWE, UAE strongly disrupted the cell walls of raw materials through cavitation effect and mechanical vibration, thereby improving mass transfer capacity. Compared with LCHP-HWE, LCHP-UAE exhibited characteristics such as lower M_W and smaller particle size, increased content of uronic acid, better thermal stability, and stronger water- and oil-holding capacities.Owing to its unique structural advantages, LCHP-UAE showed superior antioxidant activity and kidney stone inhibitory effect. In conclusion, this study establishes a robust theoretical foundation for the efficient utilization of LCH and the deployment of LCHP-UAE in contexts including the food industry and kidney stone intervention.

冬青是一种传统的中草药，具有“清热利尿、化尿排结石、解毒护肾”的功效。本研究首次建立了一种高效、绿色、经济的超声辅助提取法提取枸杞多糖（LCHP-UAE）。同时，系统地考察了UAE和热水浸提技术提取枸杞多糖的作用机理。最后，比较分析了LCHP-UAE多糖与HWE法获得的lchp多糖（LCHP-HWE）在理化性质、功能特性和生物活性方面的差异。在响应面法优化得到的最佳操作条件下，LCHP-UAE的提取率为8.25 ± 0.13 %。与HWE相比，UAE通过空化效应和机械振动强烈破坏原料细胞壁，从而提高了传质能力。与LCHP-HWE相比，LCHP-UAE表现出MW更低、粒径更小、醛酸含量更高、热稳定性更好、持水和持油能力更强等特点。由于其独特的结构优势，LCHP-UAE具有良好的抗氧化活性和抑制肾结石的作用。总之，本研究为LCH的有效利用以及LCHP-UAE在食品工业和肾结石干预等领域的应用奠定了坚实的理论基础。

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引用次数: 0

Preparation and characterization of ultrasound-mediated Ocimum basilicum L. essential oil nanoemulsion and its application in meat preservation 超声介导罗勒精油纳米乳的制备、表征及其在肉类保鲜中的应用

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-13 DOI: 10.1016/j.ultsonch.2025.107721

Yue Wang , Weixuan Pan , Xiangjun Yang , Ende Song , Hui Zhang , Yiming Liu , Kehui Wang , Huiwen Wu , Qi Liu

In this study, a stable basil oil nanoemulsion was prepared by emulsifier-assisted ultrasonication. The optimized formulation exhibited excellent physical stability under ambient, refrigerated (4 °C), and heated (55 °C) conditions. It demonstrated strong antibacterial activity against E. coli and S. aureus, inducing membrane disruption and oxidative stress, as evidenced by elevated ROS and MDA levels. The nanoemulsion also showed significant antioxidant capacity, scavenging 95 % of DPPH and 86 % of ABTS radicals and achieving a FRAP value of 14.6 µmol/g at 200 µg/mL. Cytotoxicity analysis confirmed high biocompatibility, with cell viability exceeding 83 % in L02 and BEAS-2B cells at 16 mg/mL. When applied to beef packaging, the nanoemulsion effectively maintained pH, reduced microbial counts, and suppressed TVB-N accumulation, thereby prolonging meat freshness. These findings highlight its potential as a multifunctional preservative for food and biomedical applications.

本研究采用乳化剂辅助超声法制备了稳定的罗勒油纳米乳。优化后的配方在室温、冷藏（4 °C）和加热（55 °C）条件下均表现出优异的物理稳定性。它对大肠杆菌和金黄色葡萄球菌具有很强的抗菌活性，可以诱导膜破坏和氧化应激，这可以通过提高ROS和MDA水平来证明。纳米乳还显示出显著的抗氧化能力，清除95 %的DPPH和86 %的ABTS自由基，在200 µg/mL时达到14.6 µmol/g的FRAP值。细胞毒性分析证实了高的生物相容性，在16 mg/mL浓度下，L02和BEAS-2B细胞的细胞存活率超过83 %。当应用于牛肉包装时，纳米乳有效地维持了pH值，减少了微生物数量，抑制了TVB-N的积累，从而延长了肉类的新鲜度。这些发现突出了其作为食品和生物医学用途的多功能防腐剂的潜力。

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引用次数: 0

Ultrasound and microwave assisted extraction of bioactives from food wastes: An overview on their comparative analysis towards commercialization 超声波和微波辅助提取食物垃圾中生物活性物质：商业化的比较分析综述

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-11 DOI: 10.1016/j.ultsonch.2025.107712

Arunima Nayak , Astha Shah , Sonali Bhatt , Brij Bhushan , Anuj Kumar , Rama Gaur , Inderjeet Tyagi

Valorizing food waste to functional bioactives is a robust research area. Sustainability, cost effectiveness, enhanced productivity of the bio-extracts and finally on the industrial readiness have been the driving force for research towards non-thermal extractions, novel solvents, process optimization, dual technological adoption and lately on the use of Artificial Intelligence (AI)/Machine Learning (ML). Ultrasound assisted extraction (UAE) and Microwave assisted extraction (MAE) stand apart in the context of cost-effectiveness and hold tremendous potential to be scaled-up despite the identified challenges.

Thus, with the focus on technological merit, scalability challenges, advancements, quality assessment, the present review has made an elaborate performance assessment for bioactive recovery with respect to productivity, green solvents as well as on environment and energy impact. Finally, research and development on AI tools in quality process control, optimization and real-time monitoring and combined use of UAE + MAE in addressing technological flaws, boosting quality, productivity show positive aspects towards commercialization.

将食物垃圾转化为功能性生物活性物质是一个活跃的研究领域。生物提取物的可持续性、成本效益、提高生产率以及最终的工业准备程度一直是研究非热提取、新型溶剂、工艺优化、双重技术采用以及最近使用人工智能(AI)/机器学习（ML）的推动力。超声辅助提取（UAE）和微波辅助提取（MAE）在成本效益的背景下脱颖而出，尽管存在确定的挑战，但仍具有扩大规模的巨大潜力。因此，随着技术优点，可扩展性挑战，进展，质量评估的重点，本综述从生产力，绿色溶剂以及环境和能源影响方面对生物活性回收进行了详细的性能评估。最后，在质量过程控制、优化和实时监控方面的人工智能工具的研发，以及阿联酋和MAE在解决技术缺陷、提高质量和生产力方面的联合使用，都显示出商业化的积极方面。

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引用次数: 0

Efficient extraction of bioactive compounds from Momordica charantia L. fruits using ultrasound-assisted extraction and response surface methodology optimization 超声辅助提取苦瓜果实中有效成分及响应面法优化研究

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-11 DOI: 10.1016/j.ultsonch.2025.107709

Juthaporn Ponphaiboon , Wantanwa Krongrawa , Sontaya Limmatvapirat , Chutima Limmatvapirat

This study optimized ultrasound-assisted extraction (UAE) conditions to maximize bioactive compounds from Momordica charantia L. (Okinawan bitter melon) fruit using response surface methodology (RSM). A three-factor, three-level Box-Behnken design assessed the effects of acoustic intensity, duty cycle, and extraction time on extraction yield, total phenolic content (TPC), antioxidant activity (DPPH and FRAP assays), α-glucosidase inhibitory activity, and charantin content. Acoustic intensity had the greatest impact, followed by duty cycle and extraction time. The optimal conditions were 85 W·cm⁻² acoustic intensity, 0.83 s⁻¹ duty cycle, and 13.20 min extraction time, yielding 35.80 % extract, 847.91 mg GAE/100 g extract TPC, IC₅₀ of DPPH at 119.08 µg/mL, FRAP value of 148.76 mg AAE/g extract, α-glucosidase inhibition of 41.60 mg acarbose/g extract, and charantin content of 5.83 mg/g extract. Experimental values closely matched predictions, confirming model validity. This optimized UAE method provides an efficient approach for extracting bioactive compounds from M. charantia L., ensuring high yield and biological activity. The findings offer valuable insights for industrial-scale extraction and potential applications in functional foods, pharmaceuticals, and nutraceuticals.

本研究利用响应面法（RSM）优化了冲绳苦瓜果实超声辅助提取（UAE）的工艺条件，以最大限度地提高苦瓜果实中生物活性物质的含量。采用三因素、三水平的Box-Behnken设计，评估了声强、占空比和提取时间对提取率、总酚含量（TPC）、抗氧化活性（DPPH和FRAP测定）、α-葡萄糖苷酶抑制活性和charantin含量的影响。声强的影响最大，其次是占空比和提取时间。最佳提取条件为声强85 W·cm−2，占空比0.83 s−1，提取时间13.20 min，提取率为35.80%，提取物TPC为847.91 mg GAE/100 g， DPPH IC50为119.08µg/mL， FRAP值为148.76 mg AAE/g， α-葡萄糖苷酶抑制41.60 mg阿卡波糖/g， charantin含量5.83 mg/g。实验值与预测结果非常吻合，证实了模型的有效性。优化后的UAE法是一种高效提取羊草中生物活性物质的方法，具有较高的收率和生物活性。这些发现为工业规模的提取和在功能食品、药品和营养保健品中的潜在应用提供了有价值的见解。

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引用次数: 0

Liquid-gas phase-change nanoplatforms for ultrasound-mediated cancer theranostics 超声介导癌症治疗的液气相变纳米平台

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-11 DOI: 10.1016/j.ultsonch.2025.107719

Jin Lei, Jing Lin, Peng Huang

Ultrasound has emerged as a powerful modality for cancer theranostics due to its noninvasiveness, deep-tissue penetration, and real-time feedback. However, the limited stability and short circulation time of traditional microbubble contrast agents restrict their clinical potential. Liquid-gas phase-change nanoplatforms, also known as phase-change contrast agents (PCCAs), offer a promising alternative by enabling acoustic-triggered vaporization of perfluorocarbon (PFC) cores, facilitating enhanced imaging performance and spatiotemporally controlled therapeutic functions. This review introduces the fundamental mechanisms underlying the liquid–gas phase transition, including acoustic cavitation, heating, and radiation force effects. It then summarizes the design strategies for PCCAs, covering PFC core selection (from low- to high-boiling-point types), diverse carrier platforms (lipid-, polymer-, and nanoemulsion-based systems), and surface functionalization approaches for targeted delivery and stimulus responsiveness. Subsequently, the applications of PCCAs in ultrasound-guided cancer theranostics are discussed, encompassing their roles in diagnostic imaging as well as in ultrasound-mediated mono- and combination therapies, such as chemotherapy, phototherapy, and immunotherapy. Finally, the review concludes with current challenges and future perspectives on smart material integration, multimodal imaging, and precision delivery strategies, which will be key to unlocking the full potential of PCCAs in cancer diagnosis and treatment.

超声由于其非侵入性、深入组织和实时反馈而成为癌症治疗的一种强有力的方式。然而，传统微泡造影剂稳定性有限，循环时间短，限制了其临床应用潜力。液-气相变纳米平台，也被称为相变造影剂（PCCAs），通过使全氟碳（PFC）核心的声触发汽化，促进增强的成像性能和时空控制的治疗功能，提供了一个有前途的替代方案。本文介绍了气液相变的基本机制，包括声空化、加热和辐射力效应。然后总结了PCCAs的设计策略，包括PFC核心选择（从低沸点到高沸点类型），不同的载体平台（脂质，聚合物和纳米乳基系统），以及靶向递送和刺激响应的表面功能化方法。随后，讨论了pcca在超声引导癌症治疗中的应用，包括其在诊断成像以及超声介导的单一和联合治疗（如化疗、光疗和免疫治疗）中的作用。最后，综述总结了智能材料集成、多模态成像和精确递送策略的当前挑战和未来展望，这将是释放pcca在癌症诊断和治疗中的全部潜力的关键。

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引用次数: 0

Exploring the synergistic potential of pH and ultrasonication on the functional properties of pea and lentil protein isolates and its formulation in food product 探讨pH和超声波对豌豆和扁豆分离蛋白功能特性及其食品配方的协同作用

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-10 DOI: 10.1016/j.ultsonch.2025.107718

Mehwish Arshad , Rana Muhammad Aadil , Moazzam Rafiq Khan , Abid Aslam Maan , Anubhav Pratap-Singh

The substitution of meat proteins with plant-based proteins from various sources is often motivated by nutritional considerations. However, the inherent limited solubility of plant proteins, which results in suboptimal techno-functional properties, remains a persistent challenge in food formulation. The purpose of this study was to utilize unique properties of pea (Pisum sativum L.) and lentil (Lens culinaris) through ultrasonication and pH variation in order to develop a stable and consumable protein-based product. For this purpose, pea and lentils powders were subjected to salt extraction (SE) and proceeded to further treatment with ultrasonication and pH shifting at pH 2, 4, 10 and 12 with ultrasound assisted extraction (UAE) at 20 kHz at 20 °C for 5 min for each treatment. The developed protein isolates (PIs) after these treatments were analyzed for functional properties including surface hydrophobicity, solubility, foaming capacity and stability, emulsifying activity and stability indices, turbidity and molecular weight. Among these treatments, the pH-12 treated samples showed optimal values for the functional profile, and this treatment was selected for further development of meat-analogous nuggets through assorted combinations of pea protein isolate (PPI) and lentil protein isolate (LPI) along with control treatment containing chicken nuggets. After development, nuggets were further tested for moisture content, total plate count and textural hardness for a storage period of 4 weeks along with sensory profile analyses of the developed nuggets. Meat-analogous nuggets prepared from 60 % LPI and 40 % PPI had an optimal response to texture profile and were also favored in sensory profile. This product might help the protein requirement as a vegan-based diet and enhance peas and lentils productivity into a marketable and nourishing product if it were to be developed more widely and commercialized.

用各种来源的植物性蛋白质替代肉类蛋白质通常是出于营养方面的考虑。然而，植物蛋白固有的溶解度有限，导致其技术功能性能不理想，这仍然是食品配方中一个持续的挑战。本研究的目的是利用豌豆（Pisum sativum L.）和扁豆（Lens culinaris）的独特特性，通过超声波和pH变化来开发一种稳定且可消费的蛋白基产品。为此，对豌豆和扁豆粉末进行盐提取（SE），并在pH值为2、4、10和12的情况下进行超声和pH变化的进一步处理，超声辅助提取（UAE）在20°C， 20 kHz，每次处理5分钟。分析了这些处理后的分离蛋白的功能特性，包括表面疏水性、溶解度、起泡能力和稳定性、乳化活性和稳定性指标、浊度和分子量。在这些处理中，pH-12处理的样品显示出最佳的功能特征值，并选择该处理通过豌豆分离蛋白（PPI）和扁豆分离蛋白（LPI）的组合以及含有鸡块的对照处理来进一步开发类似肉的鸡块。开发后，进一步测试了金块的水分含量、总板数和纹理硬度，并对开发后的金块进行了感官分析。由60% LPI和40% PPI制备的肉类鸡块对纹理特征的反应最佳，并且在感官特征上也受到青睐。如果该产品得到更广泛的开发和商业化，将有助于满足素食者对蛋白质的需求，并提高豌豆和小扁豆的生产力，使其成为一种适销对路的营养产品。

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引用次数: 0

Sono-electrolysis: A look into the distinguished effects of direct and indirect sonication at high frequency ultrasounds (490 kHz) 超声电解：高频超声（490khz）下直接和间接超声的不同效果观察

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-09 DOI: 10.1016/j.ultsonch.2025.107716

Kaouther Kerboua , Md Hujjatul Islam , Nour Hane Merabet , Henrik E. Hansen , Frode Seland , Odne S. Burheim , Bruno G. Pollet

This study investigates the impact of direct and indirect high-frequency (490 kHz) ultrasonication on alkaline water electrolysis for hydrogen production. Calorimetric analysis reveal that direct sonication transmits 54.17 W of acoustic power, corresponding to an efficiency of 77.39 %, whereas indirect configuration achieves substantially lower efficiencies. Hydrogen quantification shows that conventional electrolysis alone produces 0.288 mM H₂ in 60 min (0.08 mM/s), indirect sono-electrolysis improves yields modestly by 3–6 %, while direct sono-electrolysis reaches 0.777 mM in 60 min, representing a 170 % enhancement. This improvement stems from both sonolytic hydrogen generation (0.448 mM) and electrochemical synergy (0.034 mM). Electrochemical characterizations demonstrate enhanced current densities, reduced Tafel slopes, and improved kinetics for both HER and OER under direct ultrasound exposure, attributed to localized cavitation, bubble detachment, and intensified mass transport. Modeling of cavitation dynamics confirm that passing from indirect to direct sonication increases bubble compression ratios up to 8.64, resulting in order-of-magnitude increases in collapse temperature and hydrogen yields. These findings highlight the strong dependence of sono-electrolysis performance on acoustic coupling geometry, with direct configurations offering a promising pathway toward energy-efficient hydrogen production.

研究了直接和间接高频（490 kHz）超声对碱水电解制氢的影响。量热分析表明，直接声发射的声功率为54.17 W，效率为77.39%，而间接声发射的效率要低得多。氢定量分析表明，常规电解在60分钟内产生0.288 mM H2 (0.08 mM/s)，间接超声电解可略微提高产量3 - 6%，而直接超声电解在60分钟内可达到0.777 mM，提高了170%。这一改进来自于声波分解制氢（0.448 mM）和电化学协同作用（0.034 mM）。电化学表征表明，在超声直接照射下，电流密度增强，Tafel斜率降低，HER和OER动力学改善，这归因于局部空化、气泡脱离和质量传递加剧。空化动力学模型证实，从间接超声到直接超声的转换使气泡压缩比提高到8.64，从而导致崩溃温度和氢气产量的数量级提高。这些发现强调了声电解性能对声耦合几何形状的强烈依赖性，直接配置为节能制氢提供了一条有希望的途径。

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引用次数: 0

Radial oscillation of an encapsulated bubble near a planar rigid wall under dual-frequency acoustic excitation in viscoelastic fluids 粘弹性流体中双频声激励下平面刚性壁面附近封装气泡的径向振荡

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-09 DOI: 10.1016/j.ultsonch.2025.107714

Yu-Chen Zang , Di-Chao Chen , Xing-Feng Zhu , Da-Jian Wu , Wei-Jun Lin

Microbubbles play an important role in acoustic cavitation, power ultrasonics, and biomedical diagnosis and therapy. However, the influence of surrounding boundary conditions on the dynamic behaviors of an encapsulated bubble requires more in depth analysis and understanding. This study investigates the dynamic response of a lipid-coated bubble near a planar rigid wall in a viscoelastic fluid, which is transformed into a two-bubble model using the method of images. Assuming the excitation of a dual-frequency wave, the dynamic equation governing the radial oscillation is derived through combining the Rayleigh-Plesset model, the Marmottant model, and the Kelvin-Voigt model. Numerical computations are also performed for the instantaneous bubble radius in the time domain and the spectral amplitude in the frequency domain. Particular attention is paid to the pressure amplitude, the excited frequencies, the bubble-wall distance, the initial bubble radius as well as the elastic modulus and surface dilatational viscosity of the coating layer. The spectral peaks include the fundamental and higher-order resonance frequencies, accompanied by the sum frequency due to interaction between dual frequencies. The planar rigid wall significantly weakens the radial oscillation through suppressing the higher-order peaks in the frequency domain. The beating phenomenon occurs at two close frequencies with the oscillation amplitude changing periodically at the difference frequency. A longer bubble-wall distance corresponds to a weaker suppression effect of the bubble-wall interaction and thus enhances the radial oscillation. The maximum oscillation amplitude is achieved at the resonance radius but not necessarily the resonance elastic modulus of the coating layer. The findings may advantage in the design and optimization of microbubble-based systems across a wide range of applications such as ultrasonic cavitation and biomedical ultrasound.

微泡在声空化、功率超声、生物医学诊断和治疗中发挥着重要作用。但是，周围边界条件对封装气泡动力学行为的影响还需要进一步深入的分析和认识。本文研究了粘弹性流体中靠近平面刚性壁面的脂质包被气泡的动力学响应，并利用图像法将其转化为双气泡模型。以双频波为激励，结合Rayleigh-Plesset模型、Marmottant模型和Kelvin-Voigt模型，推导了控制径向振荡的动力学方程。对瞬时气泡半径在时域和频谱幅值在频域进行了数值计算。特别注意的是压力幅值、激励频率、气泡壁距离、初始气泡半径以及涂层的弹性模量和表面膨胀粘度。谱峰包括基频和高阶共振频率，伴随着双频相互作用的和频。平面刚性壁通过抑制频域的高阶峰，显著地减弱了径向振荡。振荡现象发生在两个接近的频率上，振荡幅度在差频处周期性变化。气泡壁距离越长，气泡壁相互作用的抑制作用越弱，从而增强了径向振荡。最大振荡幅度在共振半径处实现，但不一定在涂层的共振弹性模量处实现。这些发现可能有助于设计和优化基于微泡的系统，用于超声波空化和生物医学超声等广泛应用。

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引用次数: 0

Ultrasound-Assisted Deep Eutectic Solvent-Based Extraction of Polysaccharides from Okra: Optimization by Response Surface Methodology and Artificial Neural Network Modeling 超声辅助深共晶溶剂法提取秋葵多糖：响应面法和人工神经网络建模优化

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-09 DOI: 10.1016/j.ultsonch.2025.107715

Muhammad Imran , Chih-Huang Weng , Girma Sisay Wolde , Ying-Chen Chen , Yi-Jin Wu , Shang-Ming Huang , Yao-Tung Lin

Plant-derived polysaccharides are critical bioactive compounds; however, conventional extraction methods are often inefficient, energy-intensive, and may compromise their bioactivity. Ultrasound-assisted deep eutectic solvent (UA-DES) extraction offers a greener alternative by integrating acoustic cavitation with tunable solvent properties; however, optimization remains complex due to the interaction of multiple processing variables. This study reports a novel application of ultrasound-assisted deep eutectic solvent (UA-DES) extraction for okra polysaccharides (OPs), with process optimization using response surface methodology (RSM) and artificial neural network (ANN) modeling to identify optimal conditions and clarify nonlinear extraction behavior. Among the tested DES systems, choline chloride–citric acid (CCA) exhibited the highest extraction performance. Single-factor experiments and RSM identified sonication time and liquid–solid ratio as key variables. The ANN model achieved higher predictive accuracy than RSM and captured nonlinear and synergistic parameter interactions that were not evident in traditional response surfaces, providing deeper insight into process behavior. Under optimized conditions (2 h, 80 °C, 190 W, 60 mL/g), UA-DES extraction produced 23.56 % OPs and 80.75 % DPPH• scavenging activity, representing 94 % higher yield and 28 % greater antioxidant activity than hot-water ultrasonic (HWU) extraction. UA-DES-derived OPs contained higher contents of uronic acids, total sugars, and glucans, and uniquely included arabinose absent in HWU extracts. Structural analyses revealed pyranose configurations, amorphous crystallinity, and porous microstructures, which collectively contribute to improved solubility and bioactivity. Overall, UA-DES extraction using CCA provides an eco-efficient strategy for producing high-value okra polysaccharides. The integrated RSM–ANN framework enables precise optimization and enhanced mechanistic understanding, supporting UA-DES as a scalable, green technology for the production of functional polysaccharides.

植物源性多糖是重要的生物活性化合物；然而，传统的提取方法往往效率低下，能源密集，并可能损害其生物活性。超声辅助深共晶溶剂（UA-DES）萃取通过将声空化与可调溶剂性质相结合，提供了一种更环保的选择；然而，由于多个加工变量的相互作用，优化仍然是复杂的。本研究报道了超声辅助深度共晶溶剂（UA-DES）提取秋葵多糖（OPs）的新应用，并利用响应面法（RSM）和人工神经网络（ANN）建模对工艺进行优化，以确定最佳条件并阐明非线性提取行为。在所测试的DES体系中，氯化胆碱-柠檬酸（CCA）体系的萃取效果最好。单因素实验和RSM实验确定超声时间和液固比是关键变量。与RSM相比，人工神经网络模型获得了更高的预测精度，并捕获了传统响应面中不明显的非线性和协同参数相互作用，从而更深入地了解过程行为。在优化条件下（2 h, 80℃，190 W, 60 mL/g）， UA-DES提取的OPs清除率为23.56%，DPPH•清除率为80.75%，比热水超声（HWU）提取提高94%，抗氧化活性提高28%。ua - des衍生的OPs含有较高的醛酸、总糖和葡聚糖含量，并且独特地包含了HWU提取物中不存在的阿拉伯糖。结构分析揭示了吡喃糖构型，无定形结晶性和多孔微结构，这些共同有助于提高溶解度和生物活性。综上所述，用CCA提取UA-DES为生产高价值秋葵多糖提供了一种生态高效的策略。集成的RSM-ANN框架能够精确优化和增强机制理解，支持UA-DES作为可扩展的绿色技术，用于生产功能性多糖。

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引用次数: 0

Liposome-integrated ultrasound coupling subcritical water extraction for enhanced the efficiency and stability of Phyllanthus emblica polyphenols 脂质体-超声耦合亚临界水萃取法提高余甘子多酚的提取效率和稳定性。

IF 9.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry

Pub Date : 2025-12-09 DOI: 10.1016/j.ultsonch.2025.107717

Shuhui Liang , Xinyi Luo , Zexin Chen , Yushi Gong , Yong Hu , Fenglin Song , Yongguang Bi , Dongmei Li , Juan Guo

Ultrasound coupling subcritical water extraction (USWE) was developed to extract polyphenols from Phyllanthus emblica pomace (PPEPs), and extraction parameters affecting the yield of PPEPs were systematically optimized. USWE was comprehensively evaluated in terms of extraction parameters, yield, antioxidant capacity, major bioactive components, and infrared characteristics of PPEPs, and compared with four other extraction methods. Microstructural changes in the pomace were examined using scanning electron microscopy (SEM) to elucidate the interactions between the extraction process and the plant matrix. Finally, to address the issues of the instability of PPEPs and their low bioavailability, liposomal encapsulation was performed and the physicochemical properties were evaluated. Results demonstrated that the yield of PPEPs from USWE was 1.8-fold and 1.35-fold higher than those from solvent extraction (SE) and subcritical water extraction (SWE), respectively. Furthermore, PPEPs from USWE exhibited the strongest antioxidant activity and higher levels of six major bioactive compounds. SEM analysis revealed a significant increase in porosity and enlarged pores in USWE-treated pomace. Additionally, Phyllanthus emblica polyphenol liposomes (PPEPL) were prepared by thin-film dispersion technique. PPEPL showed a high encapsulation efficiency of 83.07 ± 0.09 % and retained structural integrity after 4 weeks of storage at 4 °C. PPEPL exhibited enhanced antioxidant activity compared to free PPEPs. The IC₅₀ values for scavenging DPPH·, ABTS·⁺, and hydroxyl radicals were 3.2-, 3.3-, and 1.6-fold lower, respectively. In summary, integrating USWE with liposome technology provides an efficient strategy for obtaining stable, high-quality bioactive PPEPs.

采用超声耦合亚临界水萃取法（USWE）提取余甘子果渣中的多酚，并对影响余甘子果渣得率的提取工艺参数进行了系统优化。从提取参数、得率、抗氧化能力、主要生物活性成分、红外特性等方面对USWE进行综合评价，并与其他4种提取方法进行比较。利用扫描电子显微镜（SEM）研究了渣的微观结构变化，以阐明提取过程与植物基质之间的相互作用。最后，为了解决pppep的不稳定性和生物利用度低的问题，进行了脂质体包封，并对其理化性质进行了评价。结果表明，与溶剂萃取法（SE）和亚临界水萃取法（SWE）相比，USWE法的ppps得率分别提高了1.8倍和1.35倍。此外，来自USWE的pep表现出最强的抗氧化活性和六种主要生物活性化合物的较高水平。SEM分析显示，uswe处理的渣土孔隙率显著增加，孔隙扩大。此外，采用薄膜分散法制备了余甘子多酚脂质体（PPEPL）。PPEPL包封率为83.07±0.09%，在4℃下保存4周后仍保持结构完整性。与游离ppep相比，PPEPL表现出更强的抗氧化活性。清除DPPH·、ABTS·+和羟基自由基的IC50值分别降低了3.2倍、3.3倍和1.6倍。综上所述，将USWE与脂质体技术相结合为获得稳定、高质量的生物活性ppp提供了一种有效的策略。

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