Ultrasonics Sonochemistry最新文献

{"title":"Impact of ultrasonic probe type, frequency, and static pressure on large-scale graphene exfoliation","authors":"Minhui Gao ,&nbsp;Hu Zong ,&nbsp;Lei Yu ,&nbsp;Jiacheng Yao ,&nbsp;Su Zhao ,&nbsp;Ying Zhou ,&nbsp;Yifei Li ,&nbsp;Yanyuan Zhou ,&nbsp;Jiahao Zhang ,&nbsp;Ronghe Li","doi":"10.1016/j.ultsonch.2024.107103","DOIUrl":"10.1016/j.ultsonch.2024.107103","url":null,"abstract":"<div><div>The ultrasonic liquid phase exfoliation method has emerged as an essential research direction for graphene preparation due to its cost-effectiveness and ability to minimize defects. However, this method faces challenges related to processing throughput when scaled up for industrial production. In this study, industrial grade ultrasonic homogenizers with different frequencies and probe types were evaluated for the preparation of FLG. In each experiment, 1.5 kg of graphite slurry was treated using a cyclic ultrasonic system. The results demonstrated that the 25 kHz dumbbell probe produced the thinnest FLG with the lowest defect density. Moreover, applying a static pressure of 0.2 MPa in the cycle system enhanced the cavitation-induced exfoliation of graphite sheets, effectively reducing the layer count and distribution range of FLG. This method improves the conductivity while minimizing defect density.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107103"},"PeriodicalIF":8.7,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing salt-induced gelation of egg yolk granules through pH-ultrasound combined treatment: A physicochemical and microstructural analysis","authors":"Dan Zheng ,&nbsp;Danrong Yu ,&nbsp;Shuai Lin ,&nbsp;Liting Ji ,&nbsp;Yaogui Sun ,&nbsp;Chunyou Liu ,&nbsp;Xiaoyu Zhang ,&nbsp;Zhihui Yu","doi":"10.1016/j.ultsonch.2024.107101","DOIUrl":"10.1016/j.ultsonch.2024.107101","url":null,"abstract":"<div><div>Salted duck eggs are a popular food product, but their high salt content and uneven salt distribution can reduce acceptability. This study investigated the effects of pH-high-intensity ultrasound combined treatment on the salt-induced gelation properties of egg yolk granules. The results showed that the pH5 + 150 W treatment group exhibited the best physical and gelation properties, with the smallest particle size (1597.33 nm), optimal dispersibility (PDI 0.29), and good stability. The gelation properties of this group also demonstrated excellent springiness (0.30 mm), cohesiveness (0.56), and gumminess (0.05 N). Furthermore, the pH5 + 150 W group had the highest water holding capacity of 97.42 % and relatively high hydrophobicity (173.39 μg). Notably, it also showed high oil exudation (2.83 %) and good sandiness (62.07 %). The pH5 + 150 W led to a significant redshift of the fluorescence peak at 335 nm and an enhancement of the peak intensity at 562 nm compared to the control group. Structural characterization revealed a more ordered protein structure and a uniform gel structure with enhanced electrostatic repulsion between oil droplets. Secondary structure analysis of the proteins showed a significant reduction in α-helix and β-sheet, while β-turn and random coil increased. In summary, the pH5 + 150 W treatment displayed the best gelation properties, providing theoretical guidance for enhancing the processing performance of yolk and expanding their application in the food industry.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107101"},"PeriodicalIF":8.7,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism study on the improvement of egg white emulsifying characteristic by ultrasound synergized citral: Physicochemical properties, molecular flexibility, protein structure","authors":"Pei Zhang ,&nbsp;Lan Liu ,&nbsp;Qun Huang ,&nbsp;Shugang Li ,&nbsp;Fang Geng ,&nbsp;Hongbo Song ,&nbsp;Fengping An ,&nbsp;Xin Li ,&nbsp;Yingmei Wu","doi":"10.1016/j.ultsonch.2024.107104","DOIUrl":"10.1016/j.ultsonch.2024.107104","url":null,"abstract":"<div><div>As a natural emulsifier, egg white protein (EWP) has great interfacial characteristics and high security, and has broad development prospects. This study explored the impact of ultrasound synergized citral (CI) treatment on the microstructure, molecular flexibility and emulsifying property of EWP, and predicted the interaction between CI and ovalbumin (the main protein in EWP) through molecular docking. The decrease in free amino content and the growth in molecular weight of EWP suggested that CI and proteins were successfully grafted. The results of physicochemical properties revealed that UCEWP (ultrasound synergized citral-treated EWP) had smaller particle size and larger ζ-potential absolute value, which meant that the stability of UCEWP system was enhanced. From the perspective of interfacial characteristics, UCEWP had lower interfacial tension, which remarkably improved its emulsifying property. The emulsifying activity index (EAI) and emulsifying stability index (ESI) of UCEWP were 1.99 times and 3.19 times higher than that of natural EWP (NEWP). Analysis of Fourier transform infrared spectroscopy (FT-IR) and fluorescence spectroscopy illustrated that the secondary and tertiary structures of UCEWP were more disordered and stretched than those of EWPs. Protein microstructure demonstrated that UCEWP presented loose small particle distribution, and correlation analysis reflected that the improvement of molecular flexibility was positively correlated with the enhancement of emulsifying property. These results elucidated that ultrasound synergized CI treatment is an effective mean to improve the molecular flexibility and emulsifying property of EWP, which provides a valuable reference for further application of EWP.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107104"},"PeriodicalIF":8.7,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homogenization-circulating ultrasound in combination with aqueous enzymatic pretreatment for microwave-assisted extraction of kernel oil and essential oil from the fruit of Litsea cubeba","authors":"Xinyu Yang ,&nbsp;Yang Yang ,&nbsp;Ke Zhang ,&nbsp;Ru Zhao ,&nbsp;Hao Tian ,&nbsp;Lei Yang ,&nbsp;Xiuhua Zhao","doi":"10.1016/j.ultsonch.2024.107093","DOIUrl":"10.1016/j.ultsonch.2024.107093","url":null,"abstract":"<div><div>Homogenization-circulating ultrasound in combination with an aqueous enzymatic pretreatment microwave-assisted extraction (HCUEPM) technique was successfully applied to extract kernel oil and essential oil from <em>Litsea cubeba</em> fruits. After screening the types and concentrations of enzymes, a 3 % pectinase aqueous solution was chosen. The Plackett–Burman design was used to screen eight parameters that might affect the yield of kernel oil and essential oil to identify significant variables. The best conditions were then predicted by further optimizing statistically significant factors via the Box–Behnken design. The optimal conditions were as follows: stirring speed of 1000 r/min, environmental pH of 5, homogenization time of 4 min, duty cycle of 20 %, ultrasound irradiation power of 400 W, incubation temperature of 52.78 °C, liquid–solid ratio of 9.31 mL/g, and incubation time of 2.53 h. Three parallel experiments were conducted under these conditions, yielding actual kernel oil at 240.56 ± 11.07 mL/kg DW and essential oil at 64.89 ± 3.1 mL/kg DW, which are close to the theoretical values. Compared with the HCUEPM method, the homogenization-microwave-assisted hydrodistillation (HMHD) method yielded 65.63 ± 3.2 mL/kg DW of essential oil but could not extract kernel oil. These findings demonstrate that the HCUEPM used in this study can efficiently extract a significant amount of kernel oil and essential oil from <em>L. cubeba</em> fruits in a short period of time. GC–MS analysis of the kernel oil and essential oil extracted via different methods revealed no significant differences in composition. The main components of the essential oil were <em>D</em>-limonene, <em>trans</em>-citral, <em>cis</em>-citral, and citronellal. The main components of the kernel oil were C₁₀ and C₁₂ medium-chain fatty acids, laying the foundation for the potential application of <em>L. cubeba</em> kernel oil and essential oil in the field of human health.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107093"},"PeriodicalIF":8.7,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonic-assisted membrane processes for the systematic purification of glycyrrhiza wastewater","authors":"Cunyu Li ,&nbsp;Yun Ma ,&nbsp;Shuwan Tang ,&nbsp;Yangyang Xu ,&nbsp;Xin Shen ,&nbsp;Ranyun Qiu ,&nbsp;Shenglian Cai ,&nbsp;Yanyu Ma ,&nbsp;Xinglei Zhi","doi":"10.1016/j.ultsonch.2024.107098","DOIUrl":"10.1016/j.ultsonch.2024.107098","url":null,"abstract":"&lt;div&gt;&lt;div&gt;A significant amount of glycyrrhiza wastewater is generated in the cleaning process of glycyrrhiza. The wastewater contains polysaccharide, glycyrrhizic acid, liquiritin, and other polyphenols, which is expensive for cleanup and wastes medical resources. To reduce environmental pollution from glycyrrhiza wastewater and increase the resource usage efficiency of glycyrrhiza components. According to the physicochemical properties of the component in glycyrrhiza wastewater, the ultrasonic assisted membrane separation mode was adopted to regulate the micellar state of glycyrrhizic acid and enhance the differences in membrane separation of polysaccharides, saponins, and flavones, in order to achieve the classification and separation of polysaccharides, saponins, and flavones while removing organic matter in glycyrrhiza wastewater. However, the efficiency, application, and mechanism of ultrasonic-assisted membrane technology for the separation of polysaccharides, saponins, and flavonoids from glycyrrhiza wastewater remain unclear. This study presents a green and feasible technical strategy for glycyrrhiza wastewater treatment that was developed by adjusting the parameters of ultrasonic assisted membrane separation. In this study, the systematic separation mode of ultrasonic enhanced ultrafiltration combined with nanofiltration is provided. The SCQ-9200E ultrasonic system was provided for the study with adjustable ultrasonic power, and the ultrasonic frequency was 40 kHz. The glycyrrhizic acid micelle was changed using ultrasonic power, pH, and molecular weight cut off (MWCO), and the separation differences among polysaccharide, glycyrrhizic acid, and liquiritin were enhanced. The optimal polysaccharide separation parameters used in the first step: MWCO 30 kDa, ultrasonic power 500 W and pH 5.00, and the rejections of polysaccharide, glycyrrhizic acid, and liquiritin were 87.72 %, 8.01 %, and 6.57 %, respectively. The second step included the following parameters for the separation of liquiritin and glycyrrhizic acid: MWCO 10 kDa, ultrasonic power 100 W and pH 8.00, the rejections of liquiritin and glycyrrhizic acid were 9.22 % and 40.65 %, respectively. The third step is to remove the low molecular sugar in liquiritin by nanofiltration: MWCO 800 Da, pH 8.00, retention solution diluted and separated twice, the rejection of liquiritin and total sugar were 95.72 % and 3.70 %, respectively. Ultrasonic may regulate the microtopography of glycyrrhiza wastewater with the power intensity of 50 W/L, improving the mass transfer efficiency of glycyrrhizic acid and liquiritin in the ultrafiltration separation process. As the separation volume of wastewater increased from 2.00 L to 20.00 L, the concentrations of polysaccharide, glycyrrhizic acid, and liquiritin increased by 2.5–35.4 times, 0.6–15.2 times, and 2.4–32.8 times, respectively, significantly increasing the content of index components in wastewater and solving the problem of recycling and resourc","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107098"},"PeriodicalIF":8.7,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving complexation of puerarin with kudzu starch by various ultrasonic pretreatment: Interaction mechanism analysis","authors":"Yuheng Li ,&nbsp;Chao Zhang ,&nbsp;Shuyi Li ,&nbsp;Zhenzhou Zhu ,&nbsp;Xuehua Wang ,&nbsp;Giancarlo Cravotto","doi":"10.1016/j.ultsonch.2024.107095","DOIUrl":"10.1016/j.ultsonch.2024.107095","url":null,"abstract":"<div><div>The industrial preparation of kudzu starch (KS) significantly reduces the remaining of flavonoids like puerarin (PU) in the product, weakening its biological activity and making pre-treatments on kudzu crucial. Ultrasonic technique, widely used for modifying biomolecules, can enhance nutrient interactions like those between starch and polyphenols in foods. Thus, a puerarin-kudzu starch (PKS) complex was prepared with the introduction of ultrasonic pretreatment. The results indicated that sonication increased the binding of PU to KS from 0.399 ± 0.01 to 0.609 ± 0.05 mg/g. Particle size analysis and SEM revealed that the particles of the ultrasonic puerarin-kudzu starch complex (UPKS) were larger than those of the untreated complexes. XRD, UV–vis, and FT-IR spectroscopic analyses indicated that hydrogen bonding primarily governs the interaction between PU and KS. Additionally, incorporating PU decreased the starch structure’s orderliness, while ultrasonic treatment altered the helical configuration of straight-chain starch, leading to the formation of a new, ordered structure through the creation of new hydrogen bonds. Additionally, gels formed from UPKS exhibited higher viscosity, elasticity, and shear stress, suggesting that ultrasound significantly altered the intermolecular interactions between PKS. In conclusion, the use of ultrasound under optimal conditions has demonstrated its effectiveness in preparing PKS complexes, highlighting its significant potential to produce high value-added kudzu-based products.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107095"},"PeriodicalIF":8.7,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142398944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Camel milk whey powder formulated using thermal (spray-drying process) and non-thermal (ultrasonication) processing methods: Effect on physicochemical, technological, and functional properties","authors":"Alanoud Al-Thaibani ,&nbsp;Hussein Mostafa ,&nbsp;Mariam Al Alawi ,&nbsp;Amel Sboui ,&nbsp;Fathalla Hamed ,&nbsp;Priti Mudgil ,&nbsp;Sajid Maqsood","doi":"10.1016/j.ultsonch.2024.107097","DOIUrl":"10.1016/j.ultsonch.2024.107097","url":null,"abstract":"<div><div>Whey protein concentrates (WPCs) are gaining importance as a functional ingredient due to their high technological and functional properties and their diverse application in the food industry. In this study, Camel milk whey (CW) was separated from skimmed camel milk, then either spray-dried (SD) at 170, 185 and 200 °C, or treated by ultrasonication (US) (20 kHz) for 5, 10 and 15 min followed by freeze-drying to obtain camel milk whey powder (CWP). The structural analysis of CWP was carried out by Fourier-Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD) which showed no significant difference in the functional groups profile of US samples compared to control and SD samples. US samples showed some degree of crystallinity that was comparable to the control samples, while SD samples exhibited very low degree of crystallinity. The surface morphology, particle size, and surface charge of CWP were evaluated using scanning electron microscopy (SEM) and Zetasizer. The lowest particle size of 215.1 nm with surface charge of −21.6 mv was observed in SD-185 WPC. Moreover, SD samples revealed whiter color compared to the US-treated samples which were having lower <em>L*</em> values (P &lt; 0.05). US-15 sample exhibited high protein solubility (100 %), whereas the SD-200 sample showed reduced solubility (92.7 %). Improvement in the emulsifying activity of CWP samples was observed after SD and US, with highest emulsifying activity index (EAI) values of 143.75 m²/g and 143.11 m²/g were reported for SD-185 and US-15 CWP samples, respectively. To conclude, SD and US were found to improve the physico-chemical, technological, and functional properties of CWP, and thus can be utilized as a promising strategy to preserve and enhance the technofunctional properties of CWP.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107097"},"PeriodicalIF":8.7,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study on sequential green hybrid techniques (ultrasonication, microwave and high shear homogenization) for the extraction of date seed bioactive compounds and its application as an additive for shelf-life extension of Oreochromis niloticus","authors":"Jennifer Osamede Airouyuwa ,&nbsp;Hina Khan ,&nbsp;Hussein Mostafa ,&nbsp;Priti Mudgil ,&nbsp;Sajid Maqsood","doi":"10.1016/j.ultsonch.2024.107094","DOIUrl":"10.1016/j.ultsonch.2024.107094","url":null,"abstract":"<div><div>This study focuses on the extraction of bioactive compounds from date seeds using five polyol-based deep eutectic solvents (P-DESs) in combination with hybrid green extraction techniques, specifically microwave-assisted extraction (MAE), homogenization-assisted extraction (HAE), and ultrasound-assisted extraction (UAE). The optimization of these extraction techniques was achieved using P-DESs showing the highest efficiency for extracting date seed bioactive compounds using response surface methodology (RSM) and central composite design (CCD) approach. The optimized conditions from three green techniques were further applied in the form of hybrid green extraction techniques, involving six binary and three ternary methods, to assess the percentage increase in the extraction efficiency of date seed bioactive polyphenolics. Among the five P-DESs tested, choline chloride: ethylene glycol (ChCl:Eg) exhibited the highest extraction efficiency for recovering date seed phenolic compounds. Using ChCl:Eg as the P-DES, the highest extraction efficacy was found with MAE, followed by &gt; HAE and &gt; UAE. In addition, all hybrid extraction techniques showed higher extraction efficiencies than the single extraction methods. Notably, the binary hybrid techniques combining UAE and MAE (UMAE), HAE and MAE (HMAE) resulted in significantly higher recovery of bioactive compounds, with 52 % and 49 % increases in total phenolic content, respectively, compared to single extraction techniques. The lowest MIC and MBC of P-DES (ChCl:Eg) and date seed P-DES based extract recorded against all the tested bacterial strains was 40 % and 20 % respectively. Furthermore, the date seed extract from MAE was used to extend the shelf life of <em>Oreochromis niloticus</em> stored at 4 °C for 10 days. The results indicated that the date seed polyphenolic extract effectively inhibited microbial growth in <em>Oreochromis niloticus</em> during refrigerated storage, with the total bacterial count (TBC) of all the treated samples within the recommended acceptability limit of &lt; 6 log CFU/g compared to the untreated samples, which showed a total bacterial count (TBC) &gt; 6 log CFU/g. This study demonstrated that sequential hybrid techniques enhance and intensify the recovery of bioactive compounds more effectively than any single green technique.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107094"},"PeriodicalIF":8.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-frequency ultrasound modulation of Zn2+ release from nanoclay supported ZnO antibacterial composites","authors":"Han Yi ,&nbsp;Xingyu Jiang ,&nbsp;Li Feng ,&nbsp;Liangfei Tian ,&nbsp;Huaming Yang","doi":"10.1016/j.ultsonch.2024.107096","DOIUrl":"10.1016/j.ultsonch.2024.107096","url":null,"abstract":"<div><div>Bacterial infections pose considerable health risks, emphasising the critical need for effective and biocompatible antibacterial drugs. Considerably, we developed an efficient antimicrobial system incorporating the combined potential of high-frequency ultrasound and antimicrobial drugs against bacterial infections. A ZnO–kaolinite (Kaol) composite with antibacterial properties was synthesised by growing ZnO on the Kaol nano-clay surface using the co-precipitation method. High-frequency ultrasound efficiently promotes the release of Zn²⁺, which enhances the antibacterial properties. Furthermore, in-depth in vitro antibacterial studies and bacterial live/dead staining experiments validate the exceptionally high antibacterial performance of the composite. Therefore, owing to the synergistic effects of high-frequency ultrasound and antibacterial properties, the as-prepared novel antibacterial composite is a promising potential substitute for conventional antibacterial agents.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107096"},"PeriodicalIF":8.7,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, ultrafiltration, depolymerization and gel formulation of ulvans extracted via a novel ultrasound-enzyme assisted method","authors":"K.L. Baltrusch,&nbsp;M.D. Torres,&nbsp;H. Domínguez","doi":"10.1016/j.ultsonch.2024.107072","DOIUrl":"10.1016/j.ultsonch.2024.107072","url":null,"abstract":"<div><div>Sea lettuce, or <em>Ulva</em> spp., dominates global algal biomass and significantly contributes to “green tides.”, representing a sustainable source for biomaterials. This study explores an innovative ultrasound-enzyme assisted extraction method with the novel Cellic® CTEC3 enzyme cocktail, applied for the first time in <em>Ulva</em> spp. succesfully enhancing ulvan release and extraction efficiency. Various processing methods, including ultrafiltration and dialysis, were employed to achieve higher ulvan purity. Dialyzation of ulvan resulted in a more purified product with a carbohydrate content up to 55.34 %, a sulfate content up to 21 %, and no glucose contamination. Liquid extracts were fractionated through ultrafiltration, with a 3 kDa MWCO yielding 93.51 % ulvan precipitate, representing 50.28 % of the total extractable ulvan. Sequential ultrafiltration concentrated ulvans but only partially modified their molecular weight distribution. Depolymerization using microwave and H₂O₂ shifted ulvans towards lower molecular weights, reducing high molecular weight residue. HPSEC confirmed pH-dependent aggregation behavior, with all isolated ulvans having molecular weights above 786 kDa. Hydrolysis methods were compared, with 2-hour 1 M TFA hydrolysis at 121 °C providing the best monosaccharide profile of ulvan. FTIR and NMR analyses showed preservation of sulfation. Rheology indicated biopolymeric behavior and stable gel formation. Ulvans demonstrated nutraceutical potential, being suitable for a low Na⁺ and high K⁺ diet, with a Na⁺:K⁺ ratio as low as 0.14, and were rich in Mg²⁺.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107072"},"PeriodicalIF":8.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}