Pub Date : 2024-10-29DOI: 10.1016/j.ultsonch.2024.107135
Peng Gao , Zhiyu Xu , Xinlong Zhao , Shengqi Li , Min Wang , Linkai Jing , Tao Zan
In orthopedic surgery, cortical bone cutting usually involves washing and cooling with physiological saline. However, how the saline changes the cutting behaviors of bone ultrasonic vibration cutting remains challenging. Hence, this paper simulates the clinical ultrasonic cutting condition in orthopedics to reveal the cutting behaviors of bone ultrasonic vibration orthogonal cutting immersed in physiological saline. The dynamic equation and motion process curves of ultrasonic cavitation bubbles were established. The results showed that the bone cutting immersed in physiological saline significantly improved the surface quality, reduced surface roughness and mechanical damage, and avoided large brittle cracks propagation. In saline immersed cutting, the physiological saline changes the mechanical behaviors of bone materials, resulting in plastic behaviors for the material removal and crack deflection. This study establishes the influence of physiological saline on the ultrasonic vibration cutting performance, providing guidance for orthopedic bone cutting surgery methods.
{"title":"Cutting behaviors of cortical bone ultrasonic vibration-assisted cutting immersed in physiological saline","authors":"Peng Gao , Zhiyu Xu , Xinlong Zhao , Shengqi Li , Min Wang , Linkai Jing , Tao Zan","doi":"10.1016/j.ultsonch.2024.107135","DOIUrl":"10.1016/j.ultsonch.2024.107135","url":null,"abstract":"<div><div>In orthopedic surgery, cortical bone cutting usually involves washing and cooling with physiological saline. However, how the saline changes the cutting behaviors of bone ultrasonic vibration cutting remains challenging. Hence, this paper simulates the clinical ultrasonic cutting condition in orthopedics to reveal the cutting behaviors of bone ultrasonic vibration orthogonal cutting immersed in physiological saline. The dynamic equation and motion process curves of ultrasonic cavitation bubbles were established. The results showed that the bone cutting immersed in physiological saline significantly improved the surface quality, reduced surface roughness and mechanical damage, and avoided large brittle cracks propagation. In saline immersed cutting, the physiological saline changes the mechanical behaviors of bone materials, resulting in plastic behaviors for the material removal and crack deflection. This study establishes the influence of physiological saline on the ultrasonic vibration cutting performance, providing guidance for orthopedic bone cutting surgery methods.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107135"},"PeriodicalIF":8.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-28DOI: 10.1016/j.ultsonch.2024.107129
Lu Tang , Xiaolin Liu , Shiru Bai, Dan Zhao, Xuzhen Guo, Dandan Zhu, Guiying Su, Bei Fan, Bo Wang, Liang Zhang, Fengzhong Wang
Okara protein (OP) is a potential plant-based protein that is beneficial to human health. In this work, an alternating ultrasonic/alkali treatment method with non-continued cavitation and thermal energy output was used to extract protein (AUA-OP) from okara to enhance the functional properties of OP and improve the stability of OP-based emulsions. The purity of AUA-OP was greater than 80%. Compared with traditional (physical-assisted) alkali treatment, FTIR and SDS-PAGE revealed that AUA-OP retained the chemical structure of the protein, but the number of ultrasound-induced exposure sites increased, with increased fluorescence intensity, surface hydrophobicity, and absolute ζ-potential. After alternating ultrasonic/alkali treatment, the protein particles were looser and smaller. In addition, the water/oil holding capacity, EAI, and ESI of AUA-OP further increased. The viscosity of the AUA-OP-stabilized emulsion was also greater. Finally, a 28-day emulsion storage assay revealed that the AUA-OP-stabilized emulsion was stable with a relatively low droplet size and creaming index, indicating great potential for the development of stable protein-based emulsions.
奥卡拉蛋白（OP）是一种对人类健康有益的潜在植物性蛋白。本研究采用非连续空化和热能输出的超声波/碱交替处理方法从秋葵中提取蛋白质（AUA-OP），以增强 OP 的功能特性并提高 OP 乳剂的稳定性。AUA-OP 的纯度大于 80%。与传统的（物理辅助）碱处理相比，傅立叶变换红外光谱和 SDS-PAGE 显示，AUA-OP 保留了蛋白质的化学结构，但超声诱导的暴露位点数量增加，荧光强度、表面疏水性和绝对ζ电位增加。超声波/碱交替处理后，蛋白质颗粒变得更松散、更小。此外，AUA-OP 的持水/持油能力、EAI 和 ESI 进一步提高。AUA-OP 稳定乳液的粘度也更高。最后，28 天的乳液储存试验表明，AUA-OP 稳定乳液具有相对较低的液滴大小和起泡指数，非常稳定，这表明其在开发稳定的蛋白质乳液方面具有巨大潜力。
{"title":"Okara protein extracted by alternating ultrasonic/alkali treatment and its improved physicochemical and functional properties","authors":"Lu Tang , Xiaolin Liu , Shiru Bai, Dan Zhao, Xuzhen Guo, Dandan Zhu, Guiying Su, Bei Fan, Bo Wang, Liang Zhang, Fengzhong Wang","doi":"10.1016/j.ultsonch.2024.107129","DOIUrl":"10.1016/j.ultsonch.2024.107129","url":null,"abstract":"<div><div>Okara protein (OP) is a potential plant-based protein that is beneficial to human health. In this work, an alternating ultrasonic/alkali treatment method with non-continued cavitation and thermal energy output was used to extract protein (AUA-OP) from okara to enhance the functional properties of OP and improve the stability of OP-based emulsions. The purity of AUA-OP was greater than 80%. Compared with traditional (physical-assisted) alkali treatment, FTIR and SDS-PAGE revealed that AUA-OP retained the chemical structure of the protein, but the number of ultrasound-induced exposure sites increased, with increased fluorescence intensity, surface hydrophobicity, and absolute ζ-potential. After alternating ultrasonic/alkali treatment, the protein particles were looser and smaller. In addition, the water/oil holding capacity, EAI, and ESI of AUA-OP further increased. The viscosity of the AUA-OP-stabilized emulsion was also greater. Finally, a 28-day emulsion storage assay revealed that the AUA-OP-stabilized emulsion was stable with a relatively low droplet size and creaming index, indicating great potential for the development of stable protein-based emulsions.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107129"},"PeriodicalIF":8.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-28DOI: 10.1016/j.ultsonch.2024.107131
Guillem Ferreres , Sílvia Pérez-Rafael , Ester Guaus , Òscar Palacios , Ivan Ivanov , Juan Torrent-Burgués , Tzanko Tzanov
The wearing of contact lenses (CLs) may cause bacterial infections, leading in turn to more serious complications and ultimately vision impairment. In this scenario, the first step is the adhesion of tear proteins, which provide anchoring points for bacterial colonization. A possible solution is the functionalization with an antimicrobial coating, though the latter may also lead to sight obstruction and user discomfort. In this study, adipic acid dihydrazide-modified hyaluronic acid-cobalt (II) (HA-ADH-Co) nanogels (NGs) were synthesized and deposited onto commercial CLs in a single-step sonochemical process. The coating hindered up to 60 % the protein adsorption and endowed the CLs with strong antibacterial activity against major ocular pathogens like Staphylococcus aureus and Pseudomonas aeruginosa, reducing their concentration by around 3 logs. Cytotoxicity assessment with human corneal cells demonstrated viabilities above 95 %. The nanocomposite coating did not affect the optical power and the light transmission of the CLs and provided enhanced wettability, important for the wearer comfort.
{"title":"Antimicrobial and antifouling hyaluronic acid-cobalt nanogel coatings built sonochemically on contact lenses","authors":"Guillem Ferreres , Sílvia Pérez-Rafael , Ester Guaus , Òscar Palacios , Ivan Ivanov , Juan Torrent-Burgués , Tzanko Tzanov","doi":"10.1016/j.ultsonch.2024.107131","DOIUrl":"10.1016/j.ultsonch.2024.107131","url":null,"abstract":"<div><div>The wearing of contact lenses (CLs) may cause bacterial infections, leading in turn to more serious complications and ultimately vision impairment. In this scenario, the first step is the adhesion of tear proteins, which provide anchoring points for bacterial colonization. A possible solution is the functionalization with an antimicrobial coating, though the latter may also lead to sight obstruction and user discomfort. In this study, adipic acid dihydrazide-modified hyaluronic acid-cobalt (II) (HA-ADH-Co) nanogels (NGs) were synthesized and deposited onto commercial CLs in a single-step sonochemical process. The coating hindered up to 60 % the protein adsorption and endowed the CLs with strong antibacterial activity against major ocular pathogens like <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em>, reducing their concentration by around 3 logs. Cytotoxicity assessment with human corneal cells demonstrated viabilities above 95 %. The nanocomposite coating did not affect the optical power and the light transmission of the CLs and provided enhanced wettability, important for the wearer comfort.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107131"},"PeriodicalIF":8.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-28DOI: 10.1016/j.ultsonch.2024.107134
Lei Liu , Fengnan Niu , Yating Xiong , Peng Wang , Xiaoyu Lyu , Zongyun Yang
Meat products for elderly people are high in sodium content and hardness. Thus, the aim of this study is to explore how ultrasound-assisted low-sodium salting improves meat quality and determine the conditions that result in meat tenderness ideal for people aged 65–74 years. The ultrasound-assisted treatment of bovine hip muscle with non-sodium salt (CaCl2 and C6H10CaO6) was adopted, followed by sous vide (SV) cooking (65 ℃ for 8–12 h). Results showed that ultrasound-assisted low-sodium salt curing can considerably reduce the shear force and hardness of beef (P < 0.05), promote the diffusion of Na+ (P < 0.05), and increase water content (P < 0.05), consistent with the water mobility results of low-field nuclear magnetic resonance. The tenderized effects were examined by Raman spectroscopy, which showed that the ordered α-helix and β-fold contents of the meat proteins decreased, and disordered β-turn and random coil contents increased after heat treatment with ultrasound-assisted low-sodium salt curing. Scanning electron microscopy revealed that the degree of muscle fiber destruction by ultrasound-assisted low-sodium salt curing combined with heat treatment was higher than that observed after traditional cooking with high amounts of sodium. With regard to tenderness, beef marinated with ultrasound-assisted C6H10CaO6 and subjected for 10 h was preferred by people aged 65–74 years. Ultrasound-assisted low-sodium salt curing can improvements on simultaneously reduce the sodium content and enhance the tenderness of meat products for elderly people, and provides an ultrasonic scheme for meat processing using non-sodium salt.
{"title":"Ultrasound-assisted low-sodium salt curing to modify the quality characteristics of beef for aging","authors":"Lei Liu , Fengnan Niu , Yating Xiong , Peng Wang , Xiaoyu Lyu , Zongyun Yang","doi":"10.1016/j.ultsonch.2024.107134","DOIUrl":"10.1016/j.ultsonch.2024.107134","url":null,"abstract":"<div><div>Meat products for elderly people are high in sodium content and hardness. Thus, the aim of this study is to explore how ultrasound-assisted low-sodium salting improves meat quality and determine the conditions that result in meat tenderness ideal for people aged 65–74 years. The ultrasound-assisted treatment of bovine hip muscle with non-sodium salt (CaCl<sub>2</sub> and C<sub>6</sub>H<sub>10</sub>CaO<sub>6</sub>) was adopted, followed by sous vide (SV) cooking (65 ℃ for 8–12 h). Results showed that ultrasound-assisted low-sodium salt curing can considerably reduce the shear force and hardness of beef (<em>P</em> < 0.05), promote the diffusion of Na<sup>+</sup> (<em>P</em> < 0.05), and increase water content (<em>P</em> < 0.05), consistent with the water mobility results of low-field nuclear magnetic resonance. The tenderized effects were examined by Raman spectroscopy, which showed that the ordered α-helix and β-fold contents of the meat proteins decreased, and disordered β-turn and random coil contents increased after heat treatment with ultrasound-assisted low-sodium salt curing. Scanning electron microscopy revealed that the degree of muscle fiber destruction by ultrasound-assisted low-sodium salt curing combined with heat treatment was higher than that observed after traditional cooking with high amounts of sodium. With regard to tenderness, beef marinated with ultrasound-assisted C<sub>6</sub>H<sub>10</sub>CaO<sub>6</sub> and subjected for 10 h was preferred by people aged 65–74 years. Ultrasound-assisted low-sodium salt curing can improvements on simultaneously reduce the sodium content and enhance the tenderness of meat products for elderly people, and provides an ultrasonic scheme for meat processing using non-sodium salt.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107134"},"PeriodicalIF":8.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-28DOI: 10.1016/j.ultsonch.2024.107130
Shahrzad Maleki , Elahe Abedi , Seyed Mohammad Bagher Hashemi
Experimental data were analyzed to investigate the underlying adsorption or degradation mechanism of carotenoids and chlorophylls over the bleaching of sunflower oil through different amplitudes of horn and bath ultrasound (ultrasound-assisted bleaching; UAB), temperature, time, and bleaching clay. Quantifying the color removal efficiency in both batch and continuous systems, along with the corresponding process time and energy consumption, is paramount for evaluating the energy-related performance of the treatment method. The adsorption of pigments onto activated bentonite was notably rapid and effective when ultrasound was employed. In both bleaching processes, the adsorption kinetics of carotenoids and chlorophyll exhibited a pseudo-second-order behavior. At the same time, a pseudo-first-order equation provided a better fit for the control conditions. Moreover, intra-particle diffusion contributed to the adsorption mechanism, although it was not the only rate-limiting step in the adsorption of pigments on the clay. It is hypothesized that carotenoid and chlorophyll adsorption occur through physisorption in control conditions, while chemical reactions play a role in pigment removal under sonication. The Freundlich isotherm yielded precise estimates of the adsorption equilibrium data for carotenoid and chlorophyll during ultrasonic bleaching, suggesting a multilayer adsorption mechanism under ultrasound exposure. The thermodynamic study found that pigment adsorption was feasible, spontaneous, and endothermic. According to the results, horn and bath ultrasound, especially at higher voltages, can remarkably remove carotenoid and chlorophyll from sunflower oil compared to the traditional bleaching process.
{"title":"Insights into kinetic, isotherm, and thermodynamic of ultrasound mode- and amplitude-dependent carotenoid and chlorophyll degradation or/and adsorption","authors":"Shahrzad Maleki , Elahe Abedi , Seyed Mohammad Bagher Hashemi","doi":"10.1016/j.ultsonch.2024.107130","DOIUrl":"10.1016/j.ultsonch.2024.107130","url":null,"abstract":"<div><div>Experimental data were analyzed to investigate the underlying adsorption or degradation mechanism of carotenoids and chlorophylls over the bleaching of sunflower oil through different amplitudes of horn and bath ultrasound (ultrasound-assisted bleaching; UAB), temperature, time, and bleaching clay. Quantifying the color removal efficiency in both batch and continuous systems, along with the corresponding process time and energy consumption, is paramount for evaluating the energy-related performance of the treatment method. The adsorption of pigments onto activated bentonite was notably rapid and effective when ultrasound was employed. In both bleaching processes, the adsorption kinetics of carotenoids and chlorophyll exhibited a pseudo-second-order behavior. At the same time, a pseudo-first-order equation provided a better fit for the control conditions. Moreover, intra-particle diffusion contributed to the adsorption mechanism, although it was not the only rate-limiting step in the adsorption of pigments on the clay. It is hypothesized that carotenoid and chlorophyll adsorption occur through physisorption in control conditions, while chemical reactions play a role in pigment removal under sonication. The Freundlich isotherm yielded precise estimates of the adsorption equilibrium data for carotenoid and chlorophyll during ultrasonic bleaching, suggesting a multilayer adsorption mechanism under ultrasound exposure. The thermodynamic study found that pigment adsorption was feasible, spontaneous, and endothermic. According to the results, horn and bath ultrasound, especially at higher voltages, can remarkably remove carotenoid and chlorophyll from sunflower oil compared to the traditional bleaching process.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107130"},"PeriodicalIF":8.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anchusa italica Retz. Flowers (Airfs) is a perennial herb belonging to the Boraginaceae family. A variety of pharmacological agents are employed in the treatment of a multitude of cardiovascular disorders. The study employs ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-Exactive Orbitrap-MS) to elucidate the complex chemical composition of these flowers, identifying a multitude of bioactive constituents. It is noteworthy that rosmarinic acid (RA) emerged as a predominant compound, a finding underscored by its high relative content of 39.8511% in the methanolic extract of the flowers.
The innovative approach involved the use of a deep eutectic solvent (DES), a green extraction medium, which, when combined with ultrasonic assistance, resulted in a notable enhancement of the extraction yield of total polyphenols and RA. The optimization of this extraction process was achieved through a meticulous response surface methodology, which identified critical factors and their optimal conditions. These included a water content of 29.051 % and a material-liquid ratio of 20.961 mL/g. The optimal conditions were determined to be an extraction time of 35.725 min, an ultrasonic power of 240.342 W.
In vitro antioxidant assays further substantiated the therapeutic potential of Anchusa italica Retz. flowers. RA demonstrated remarkable DPPH and ABTS radical scavenging capabilities, with rates exceeding 90%. The comprehensive evaluation value (CEV), derived from the total polyphenol content (TPC) and RA, was optimized under the aforementioned conditions, yielding a CEV of 222.452.
The optimized extraction method presents a sustainable and efficient protocol for the isolation of bioactive compounds from natural sources, thereby underscoring the significance of this research in the field of natural product chemistry and ultrasonics Sonochemistry.
Anchusa italica Retz.花（Airfs）属于婆婆纳科多年生草本植物。在治疗多种心血管疾病中使用了多种药剂。本研究采用超高效液相色谱-四极杆飞行时间质谱法（UPLC-Q-Exactive Orbitrap-MS）来阐明这些花的复杂化学成分，并鉴定出多种生物活性成分。值得注意的是，迷迭香酸（RA）是一种主要化合物，其在花的甲醇提取物中的相对含量高达 39.8511%，这一发现凸显了这一点。创新方法包括使用深共晶溶剂（DES）这种绿色萃取介质，并结合超声波辅助，显著提高了总多酚和 RA 的萃取率。该萃取工艺的优化是通过细致的响应面方法实现的，该方法确定了关键因素及其最佳条件。这些因素包括 29.051 % 的含水量和 20.961 mL/g 的料液比。体外抗氧化试验进一步证实了 Anchusa italica Retz.RA 具有出色的 DPPH 和 ABTS 自由基清除能力，清除率超过 90%。根据总多酚含量（TPC）和 RA 得出的综合评价值（CEV）在上述条件下得到了优化，CEV 为 222.452。优化后的萃取方法为从天然资源中分离生物活性化合物提供了一种可持续的高效方案，从而凸显了该研究在天然产物化学和超声声化学领域的重要意义。
{"title":"Study on ultrasonic-assisted deep eutectic solvent extraction process and in vitro antioxidant of Anchusa italica Retz. Flowers","authors":"Linyang Wang , Pin Chen , Ainiwaer Aikemu , Haiyan Zhang , Shuge Tian","doi":"10.1016/j.ultsonch.2024.107127","DOIUrl":"10.1016/j.ultsonch.2024.107127","url":null,"abstract":"<div><div><em>Anchusa italica</em> Retz. Flowers (Airfs) is a perennial herb belonging to the Boraginaceae family. A variety of pharmacological agents are employed in the treatment of a multitude of cardiovascular disorders. The study employs ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UPLC-Q-Exactive Orbitrap-MS) to elucidate the complex chemical composition of these flowers, identifying a multitude of bioactive constituents. It is noteworthy that rosmarinic acid (RA) emerged as a predominant compound, a finding underscored by its high relative content of 39.8511% in the methanolic extract of the flowers.</div><div>The innovative approach involved the use of a deep eutectic solvent (DES), a green extraction medium, which, when combined with ultrasonic assistance, resulted in a notable enhancement of the extraction yield of total polyphenols and RA. The optimization of this extraction process was achieved through a meticulous response surface methodology, which identified critical factors and their optimal conditions. These included a water content of 29.051 % and a material-liquid ratio of 20.961 mL/g. The optimal conditions were determined to be an extraction time of 35.725 min, an ultrasonic power of 240.342 W.</div><div>In vitro antioxidant assays further substantiated the therapeutic potential of Anchusa italica Retz. flowers. RA demonstrated remarkable DPPH and ABTS radical scavenging capabilities, with rates exceeding 90%. The comprehensive evaluation value (CEV), derived from the total polyphenol content (TPC) and RA, was optimized under the aforementioned conditions, yielding a CEV of 222.452.</div><div>The optimized extraction method presents a sustainable and efficient protocol for the isolation of bioactive compounds from natural sources, thereby underscoring the significance of this research in the field of natural product chemistry and ultrasonics Sonochemistry.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107127"},"PeriodicalIF":8.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-28DOI: 10.1016/j.ultsonch.2024.107133
Faiza Javed , Saima Tehseen , Faiza Ashfaq , Aysha Sameen , Waseem Khalid , Rizwana Batool , Ahmed Bilal , Muhammad Zubair Khalid , Tawfiq Alsulami , Robert Mugabi , Gulzar Ahmad Nayik
Fig fruit (Ficus carica L.) drink is a source of healthy minerals, vitamins, and bioactive ingredients however to improve the shelf-life of functional drink naturally, moringa leaf extract was compared with optimized concentration of potassium metabisulphite (synthetic preservative). Purposely, fig fruit drink, without preservatives was considered as negative control whereas, 0.2 % potassium metabisulphite-based fig fruit drink was taken as positive control. Further, ultrasound assisted extracts of moringa at varied levels; 5, 10, 15, and 20 % were incorporated in the fig fruit drink as natural preservative to test antioxidant, storage, and sensory quality against control samples. Resultantly, the maximum loss in antioxidant activity (18–38 %) and functional ingredients (24–56 %) was observed in negative control sample, in response to high microbial expansion till the termination of the study. Additionally, acceptability score for negative control sample was maximum at Day 1, that afterwards faced significant decline at 30th Day (6.6 ± 0.3). In contrast, positive control sample demonstrated minimum loss of free radical scavenging ability (7–22 %), polyphenols (11 %) and flavonoids (7 %) thus indicated maximum control on microbes i.e. 61–63 % as compared to negative control. Further, positive control sample indicated optimum consumer preference (7.0 ± 0.3) that remained stable throughout storage. Further, as the concentration of moringa exceeded from 5 to 20 %, the loss of functional ingredients reduced from 13 to 24 to 6–11 % and deterioration in antioxidant capacity suppressed from 14 to 26 to 8–20 %, correspondingly however, the sensory acceptability showed a declining trend, and 20 % moringa based sample portrayed poor consumer response (5.0 ± 0.2). Lastly, it was deduced that control on microbes was directly proportional to the concentration of moringa extract in fig fruit drink, that was poor in 5 % moringa extract concentration; 32–54 %. Conclusively, customer preference was reasonable (6 ± 0) at 15 % moringa extract concentration so this level should be employed in fig fruit drink for realistic control on bacterial (57 %) and fungal (47 %) activities.
{"title":"Stabilization of Ficus carica L. Drink by utilizing varying levels of ultrasound-assisted moringa extract as a natural preservative","authors":"Faiza Javed , Saima Tehseen , Faiza Ashfaq , Aysha Sameen , Waseem Khalid , Rizwana Batool , Ahmed Bilal , Muhammad Zubair Khalid , Tawfiq Alsulami , Robert Mugabi , Gulzar Ahmad Nayik","doi":"10.1016/j.ultsonch.2024.107133","DOIUrl":"10.1016/j.ultsonch.2024.107133","url":null,"abstract":"<div><div>Fig fruit (<em>Ficus carica</em> L.) drink is a source of healthy minerals, vitamins, and bioactive ingredients however to improve the shelf-life of functional drink naturally, moringa leaf extract was compared with optimized concentration of potassium metabisulphite (synthetic preservative). Purposely, fig fruit drink, without preservatives was considered as negative control whereas, 0.2 % potassium metabisulphite-based fig fruit drink was taken as positive control. Further, ultrasound assisted extracts of moringa at varied levels; 5, 10, 15, and 20 % were incorporated in the fig fruit drink as natural preservative to test antioxidant, storage, and sensory quality against control samples. Resultantly, the maximum loss in antioxidant activity (18–38 %) and functional ingredients (24–56 %) was observed in negative control sample, in response to high microbial expansion till the termination of the study. Additionally, acceptability score for negative control sample was maximum at Day 1, that afterwards faced significant decline at 30th Day (6.6 ± 0.3). In contrast, positive control sample demonstrated minimum loss of free radical scavenging ability (7–22 %), polyphenols (11 %) and flavonoids (7 %) thus indicated maximum control on microbes i.e. 61–63 % as compared to negative control. Further, positive control sample indicated optimum consumer preference (7.0 ± 0.3) that remained stable throughout storage. Further, as the concentration of moringa exceeded from 5 to 20 %, the loss of functional ingredients reduced from 13 to 24 to 6–11 % and deterioration in antioxidant capacity suppressed from 14 to 26 to 8–20 %, correspondingly however, the sensory acceptability showed a declining trend, and 20 % moringa based sample portrayed poor consumer response (5.0 ± 0.2). Lastly, it was deduced that control on microbes was directly proportional to the concentration of moringa extract in fig fruit drink, that was poor in 5 % moringa extract concentration; 32–54 %. Conclusively, customer preference was reasonable (6 ± 0) at 15 % moringa extract concentration so this level should be employed in fig fruit drink for realistic control on bacterial (57 %) and fungal (47 %) activities.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107133"},"PeriodicalIF":8.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-28DOI: 10.1016/j.ultsonch.2024.107132
Feifan Leng , Yubo Wang , Ning Zhu , Xiaopeng Guo , Wen Luo , Yonggang Wang
Ultrasonic-mediated plasmid transformation is a promising microbial transformation strategy with broad application prospects that has attracted interest across various fields. Limited research exists on developing a quantitative model to understand the relationship between transformation efficiency and ultrasonic power. Within the ultrasonic range that did not damage plasmids, the maximum transformation efficiency reached at 4.84 × 105 CFU/μg DNA. A kinetic model based on changes in membrane permeability was utilized to determine the membrane permeability at different power levels. The results indicated a linear correlation between ultrasonic power, transformation efficiency, and membrane permeability within a specific range. A quantitative relationship model was established based on ultrasonic power and transformation efficiency in E. coli. Electron microscopy revealed that E. coli cells subjected to ultrasonic treatment exhibited pore formation and cellular expansion. Furthermore, the integrity of the bacterial membrane was compromised as ultrasonic power increased. Nine genes associated with the functional terms of cell membrane components and transmembrane transport were identified in E. coli DH5α. According to qRT-PCR results, genes with these functions (including cusC, uidC, tolQ, tolA, ompC, yaiY) play crucial roles in ultrasound-mediated transformation of E. coli DH5α. This study suggested that ultrasound-mediated transformation in E. coli DH5α is not a simple physical–chemical process but rather involves the regulation of responsive membrane-related genes. This research establishes the groundwork for future comprehensive investigations into the molecular mechanism of ultrasound-mediated transformation and provides insights for the application of ultrasound technology in genetic engineering and related fields.
{"title":"Development and mechanism exploration of a quantitative model for Escherichia coli transformation efficiency based on ultrasonic power","authors":"Feifan Leng , Yubo Wang , Ning Zhu , Xiaopeng Guo , Wen Luo , Yonggang Wang","doi":"10.1016/j.ultsonch.2024.107132","DOIUrl":"10.1016/j.ultsonch.2024.107132","url":null,"abstract":"<div><div>Ultrasonic-mediated plasmid transformation is a promising microbial transformation strategy with broad application prospects that has attracted interest across various fields. Limited research exists on developing a quantitative model to understand the relationship between transformation efficiency and ultrasonic power. Within the ultrasonic range that did not damage plasmids, the maximum transformation efficiency reached at 4.84 × 10<sup>5</sup> CFU/μg DNA. A kinetic model based on changes in membrane permeability was utilized to determine the membrane permeability at different power levels. The results indicated a linear correlation between ultrasonic power, transformation efficiency, and membrane permeability within a specific range. A quantitative relationship model was established based on ultrasonic power and transformation efficiency in <em>E. coli</em>. Electron microscopy revealed that <em>E. coli</em> cells subjected to ultrasonic treatment exhibited pore formation and cellular expansion. Furthermore, the integrity of the bacterial membrane was compromised as ultrasonic power increased. Nine genes associated with the functional terms of cell membrane components and transmembrane transport were identified in <em>E. coli</em> DH5α. According to qRT-PCR results, genes with these functions (including <em>cusC, uidC, tolQ, tolA, ompC, yaiY)</em> play crucial roles in ultrasound-mediated transformation of <em>E. coli</em> DH5α<em>.</em> This study suggested that ultrasound-mediated transformation in <em>E. coli</em> DH5α is not a simple physical–chemical process but rather involves the regulation of responsive membrane-related genes. This research establishes the groundwork for future comprehensive investigations into the molecular mechanism of ultrasound-mediated transformation and provides insights for the application of ultrasound technology in genetic engineering and related fields.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107132"},"PeriodicalIF":8.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-26DOI: 10.1016/j.ultsonch.2024.107128
Mohammed Aldholmi
Dolomiaea costus, commonly known as Indian costus, is a medicinal plant from the Asteraceae family. The root and powder of costus have been widely used to treat various health conditions. The primary bioactive compounds in this plant are sesquiterpene lactones, particularly costunolide and dehydrocostus lactone. This study aimed to establish a rapid, environmentally friendly, and cost-effective method for the high-throughput extraction and quantification of sesquiterpene lactones in Indian costus. Ultrasonic bath (UB) and UPLC/MS-MS were employed to extract and analyse 49 Indian costus samples. Aqueous ethanol was identified as the most effective solvent system for extracting and analysing sesquiterpene lactones. The extraction efficiency of the ultrasonic bath was comparable to that of the ultrasonic homogeniser while shaking showed the lowest efficiency. The environmentally friendly UPLC/MS-MS analysis revealed mean concentrations (±SD; μg/100 μg) of 1.00 (±0.39) for costunolide and 0.70 (±0.25) for dehydrocostus lactone. An inverse correlation was observed between sesquiterpene lactone content and sample colour. Most samples contained costunolide levels above the minimum limit (0.6 %) specified by the Chinese monograph, but only a few met the 1.8 % threshold for total sesquiterpene lactones. Given the importance of bioactive sesquiterpene lactones for medicinal efficacy, insufficient levels may result in diminished therapeutic value. Therefore, standardising Indian costus products is crucial to ensure quality and appropriate dosing. This study contributes to the standardisation of Indian costus, a vital step towards ensuring the efficacy and safety of herbal products.
{"title":"Method development and validation for the extraction and quantification of sesquiterpene lactones in Dolomiaea costus","authors":"Mohammed Aldholmi","doi":"10.1016/j.ultsonch.2024.107128","DOIUrl":"10.1016/j.ultsonch.2024.107128","url":null,"abstract":"<div><div><em>Dolomiaea costus</em>, commonly known as Indian costus, is a medicinal plant from the Asteraceae family. The root and powder of costus have been widely used to treat various health conditions. The primary bioactive compounds in this plant are sesquiterpene lactones, particularly costunolide and dehydrocostus lactone. This study aimed to establish a rapid, environmentally friendly, and cost-effective method for the high-throughput extraction and quantification of sesquiterpene lactones in Indian costus. Ultrasonic bath (UB) and UPLC/MS-MS were employed to extract and analyse 49 Indian costus samples. Aqueous ethanol was identified as the most effective solvent system for extracting and analysing sesquiterpene lactones. The extraction efficiency of the ultrasonic bath was comparable to that of the ultrasonic homogeniser while shaking showed the lowest efficiency. The environmentally friendly UPLC/MS-MS analysis revealed mean concentrations (±SD; μg/100 μg) of 1.00 (±0.39) for costunolide and 0.70 (±0.25) for dehydrocostus lactone. An inverse correlation was observed between sesquiterpene lactone content and sample colour. Most samples contained costunolide levels above the minimum limit (0.6 %) specified by the Chinese monograph, but only a few met the 1.8 % threshold for total sesquiterpene lactones. Given the importance of bioactive sesquiterpene lactones for medicinal efficacy, insufficient levels may result in diminished therapeutic value. Therefore, standardising Indian costus products is crucial to ensure quality and appropriate dosing. This study contributes to the standardisation of Indian costus, a vital step towards ensuring the efficacy and safety of herbal products.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107128"},"PeriodicalIF":8.7,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-23DOI: 10.1016/j.ultsonch.2024.107125
Ya Su , Shuyi Li , Tiantian Hu , Jieru Zhang , S.M. Roknul Azam , Aiqing Ren
The effects of integrated ultrasonic infrared frying (USIF) on the oil absorption of apple slices and the oil deterioration were studied with frequency of 28 and 40 kHz, respectively. Results showed that the heat transfer and moisture migration was accelerated by the integrated ultrasound in IF. The soluble Gal-A content and esterification degree of pectin was increased, the damages of pectin crystal structure and chemical structure in side chain was aggravated. These damages to pectin were intensified with higher frequency (40 kHz) of ultrasound. Lower retention of phenols was found in USIF apple slices, but the flavonoids content had no significant change compared to CF samples. USIF samples showed a smoother morphology, and the pore volume and porosity were reduced by ultrasonication applied with 28 kHz but increased with 40 kHz. The largest volume fraction of pores was changed from 100-250 μm in IF to 0.02–10 μm and 10–100 μm by the integrated ultrasound at 28 kHz and 40 kHz samples, respectively. The total oil uptake in USIF samples was reduced by 24.9 %–33.2 % compared to the conventional fried (CF) samples, and achieved the lowest with the frequency of 40 kHz. The surficial and structural oil were also decreased by 39.2 %-51.3 % and 20.9 %–32.3 %, respectively. The peroxide value, acid value, carbonyl value, polar component, and the saturated fatty acids ratio of oil in repeated frying for 16 h was reduced in USIF, especially with ultrasonication 40 kHz. These results indicate that USIF is a promising method for producing novel low-oil apple fries.
{"title":"Reducing the oil absorption and oil deterioration in fried apple slices by ultrasound integrated in infrared frying","authors":"Ya Su , Shuyi Li , Tiantian Hu , Jieru Zhang , S.M. Roknul Azam , Aiqing Ren","doi":"10.1016/j.ultsonch.2024.107125","DOIUrl":"10.1016/j.ultsonch.2024.107125","url":null,"abstract":"<div><div>The effects of integrated ultrasonic infrared frying (USIF) on the oil absorption of apple slices and the oil deterioration were studied with frequency of 28 and 40 kHz, respectively. Results showed that the heat transfer and moisture migration was accelerated by the integrated ultrasound in IF. The soluble Gal-A content and esterification degree of pectin was increased, the damages of pectin crystal structure and chemical structure in side chain was aggravated. These damages to pectin were intensified with higher frequency (40 kHz) of ultrasound. Lower retention of phenols was found in USIF apple slices, but the flavonoids content had no significant change compared to CF samples. USIF samples showed a smoother morphology, and the pore volume and porosity were reduced by ultrasonication applied with 28 kHz but increased with 40 kHz. The largest volume fraction of pores was changed from 100-250 μm in IF to 0.02–10 μm and 10–100 μm by the integrated ultrasound at 28 kHz and 40 kHz samples, respectively. The total oil uptake in USIF samples was reduced by 24.9 %–33.2 % compared to the conventional fried (CF) samples, and achieved the lowest with the frequency of 40 kHz. The surficial and structural oil were also decreased by 39.2 %-51.3 % and 20.9 %–32.3 %, respectively. The peroxide value, acid value, carbonyl value, polar component, and the saturated fatty acids ratio of oil in repeated frying for 16 h was reduced in USIF, especially with ultrasonication 40 kHz. These results indicate that USIF is a promising method for producing novel low-oil apple fries.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"111 ","pages":"Article 107125"},"PeriodicalIF":8.7,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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