Pub Date : 2025-11-14DOI: 10.1016/j.ultsonch.2025.107680
Wen Zhao , Jintao Du , Yunxuan Song , Zilin Ma , Songyan Li , Yuhua Wei , Guiqiang Zhang
The immunotherapeutic potential of sonodynamic therapy (SDT) is hindered by tumor defense mechanisms driven by glutamine metabolism, including glutathione (GSH)-dependent redox homeostasis and an immunosuppressive tumor microenvironment (TME). To address these challenges, we herein developed metabolic nanoblockers (CBE@AM NPs) by encapsulating a glutamine metabolism inhibitor CB839 and a sonosensitizer chlorin e6 (Ce6) into melanin-inspired nanoparticles. The resulting nanoblockers triggered robust reactive oxygen species (ROS) production upon ultrasound irradiation, thereby destroying tumor cells and inducing immunogenic cell death (ICD). Concurrently, they inhibited the glutamine metabolism in the tumor, disrupting redox homeostasis and remodeling the immunosuppressive TME, thereby amplifying both SDT-generated oxidative stress and ICD-induced antitumor immunity. CBE@AM NPs demonstrated a potent tumor-inhibitory effect in tumor-bearing mice, highlighting their potential for immunometabolic reprogramming to enhance the therapeutic efficacy of SDT.
{"title":"Metabolic nanoblockers for synergistic cancer treatment through glutaminase inhibition and sonodynamic therapy","authors":"Wen Zhao , Jintao Du , Yunxuan Song , Zilin Ma , Songyan Li , Yuhua Wei , Guiqiang Zhang","doi":"10.1016/j.ultsonch.2025.107680","DOIUrl":"10.1016/j.ultsonch.2025.107680","url":null,"abstract":"<div><div>The immunotherapeutic potential of sonodynamic therapy (SDT) is hindered by tumor defense mechanisms driven by glutamine metabolism, including glutathione (GSH)-dependent redox homeostasis and an immunosuppressive tumor microenvironment (TME). To address these challenges, we herein developed metabolic nanoblockers (CBE@AM NPs) by encapsulating a glutamine metabolism inhibitor CB839 and a sonosensitizer chlorin e6 (Ce6) into melanin-inspired nanoparticles. The resulting nanoblockers triggered robust reactive oxygen species (ROS) production upon ultrasound irradiation, thereby destroying tumor cells and inducing immunogenic cell death (ICD). Concurrently, they inhibited the glutamine metabolism in the tumor, disrupting redox homeostasis and remodeling the immunosuppressive TME, thereby amplifying both SDT-generated oxidative stress and ICD-induced antitumor immunity. CBE@AM NPs demonstrated a potent tumor-inhibitory effect in tumor-bearing mice, highlighting their potential for immunometabolic reprogramming to enhance the therapeutic efficacy of SDT.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107680"},"PeriodicalIF":9.7,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145531117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-14DOI: 10.1016/j.ultsonch.2025.107682
Juncheng Hu , Wenyu Nie , Suxu Zhao , Yong Liu , Hengyi Zhu , Shijie Tu , Jiawei Zhang , Kris Y. Yang , Ning Xue , Justin Z. Lian , Bin Dong , Stefano Cucurachi , Yuan Gao
Owing to their excellent biocompatibility and antibacterial properties, the global annual production of silver nanoparticles (AgNPs) is estimated at 400–800 tons. Therefore, developing a green and safe approach for AgNPs synthesis is urgently required. However, the conventional AgNPs manufacturing methods required toxic reductant and higher energy consumption, which makes the reaction system harmful to humans and environment. In this study, an eco-friendly ultrasound-intensified continuous system employing minimal toxic reagents was developed. The AgNPs formation process was intensified by cavitation instead of using toxic and strong reductants. Several analytical methods were conducted to systematically investigate the mechanism and performance of as-synthesized AgNPs, such as particle size distribution, zeta potential, UV–visible absorption, morphology, yield calculation, as well as in vitro photothermal, and antibacterial. The results clearly indicated that the physicochemical properties of AgNPs could be tuned by adjusting process parameters, including temperature, feeding rate, and ultrasonic power. Specifically, the AgNPs prepared at 90 ℃, 600 W and 50 µL/min exhibited smaller size (2–5 nm) with monodisperse, spherical shape, higher yield, enhanced photothermal, and antibacterial activities. Furthermore, life-cycle assessment (LCA) and machine learning (ML) were employed to evaluate the process sustainability and identify the key influencing parameters. Comprehensive consideration of safety, experimental results, and LCA outcomes demonstrated that the ultrasound-intensified continuous method proposed in this study is superior to the traditional NaBH4-based batch synthesis at the laboratory scale.
{"title":"Eco-friendly production of AgNPs by ultrasound-intensified continuous method, and process evaluation via life cycle assessment and machine learning","authors":"Juncheng Hu , Wenyu Nie , Suxu Zhao , Yong Liu , Hengyi Zhu , Shijie Tu , Jiawei Zhang , Kris Y. Yang , Ning Xue , Justin Z. Lian , Bin Dong , Stefano Cucurachi , Yuan Gao","doi":"10.1016/j.ultsonch.2025.107682","DOIUrl":"10.1016/j.ultsonch.2025.107682","url":null,"abstract":"<div><div>Owing to their excellent biocompatibility and antibacterial properties, the global annual production of silver nanoparticles (AgNPs) is estimated at 400–800 tons. Therefore, developing a green and safe approach for AgNPs synthesis is urgently required. However, the conventional AgNPs manufacturing methods required toxic reductant and higher energy consumption, which makes the reaction system harmful to humans and environment. In this study, an eco-friendly ultrasound-intensified continuous system employing minimal toxic reagents was developed. The AgNPs formation process was intensified by cavitation instead of using toxic and strong reductants. Several analytical methods were conducted to systematically investigate the mechanism and performance of as-synthesized AgNPs, such as particle size distribution, zeta potential, UV–visible absorption, morphology, yield calculation, as well as <em>in vitro</em> photothermal, and antibacterial. The results clearly indicated that the physicochemical properties of AgNPs could be tuned by adjusting process parameters, including temperature, feeding rate, and ultrasonic power. Specifically, the AgNPs prepared at 90 ℃, 600 W and 50 µL/min exhibited smaller size (2–5 nm) with monodisperse, spherical shape, higher yield, enhanced photothermal, and antibacterial activities. Furthermore, life-cycle assessment (LCA) and machine learning (ML) were employed to evaluate the process sustainability and identify the key influencing parameters. Comprehensive consideration of safety, experimental results, and LCA outcomes demonstrated that the ultrasound-intensified continuous method proposed in this study is superior to the traditional NaBH<sub>4</sub>-based batch synthesis at the laboratory scale.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107682"},"PeriodicalIF":9.7,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145531114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-13DOI: 10.1016/j.ultsonch.2025.107679
Zheng Li , Weibin You , Sivakumar Manickam , Haiyan Bie , Wenlong Wang , Xun Sun
To effectively eliminate excess antibiotics from aqueous environments and to mitigate the dissemination of antibiotic resistance genes (ARGs), this study proposes a novel degradation system that activates peroxymonosulfate (PMS) through a synergistic combination of hydrodynamic cavitation (HC) and divalent copper ions (Cu2+). Levofloxacin (LEV) is employed as the representative target contaminant to evaluate the system’s performance. HC has emerged as a promising technique for pollutant removal. In this study, the localized high-temperature and high-pressure conditions generated by HC not only partially activated PMS but also facilitated its interaction with Cu2+ ions, leading to a pronounced synergistic enhancement in sulfate radical (SO4−) generation and efficient pollutant degradation. Under optimized HC/Cu2+/PMS conditions (Cu2+ = 5 mM, PMS = 2.5 mM, inlet pressure = 0.15 MPa, pH = 10), complete removal of LEV (30 mg/L) was achieved within 50 min. This study elucidates the degradation mechanisms and pathways of LEV within the coupled HC/Cu2+/PMS system and evaluates the ecological safety of its degradation intermediates using the U.S. EPA’s T.E.S.T. (Toxicity Estimation Software Tool). Furthermore, the system’s applicability was validated through degradation experiments involving a range of representative pollutants, demonstrating its broad-spectrum effectiveness. Crucially, the HC/Cu2+/PMS system demonstrated a superior cavitation yield (2.78 × 10−5 mg/J) and a low electrical energy per order (EE/O) of 229.48 kWh/m3, highlighting its high energy efficiency and practical potential for sustainable wastewater treatment. The experimental results emphasize the system’s strong potential for the effective removal of organic pollutants from water, offering a novel and sustainable approach for advanced water treatment.
{"title":"Synergistic degradation of levofloxacin (LEV) by Cu2+-activated peroxymonosulfate (PMS) under hydrodynamic cavitation (HC): Efficiency and mechanistic insights","authors":"Zheng Li , Weibin You , Sivakumar Manickam , Haiyan Bie , Wenlong Wang , Xun Sun","doi":"10.1016/j.ultsonch.2025.107679","DOIUrl":"10.1016/j.ultsonch.2025.107679","url":null,"abstract":"<div><div>To effectively eliminate excess antibiotics from aqueous environments and to mitigate the dissemination of antibiotic resistance genes (ARGs), this study proposes a novel degradation system that activates peroxymonosulfate (PMS) through a synergistic combination of hydrodynamic cavitation (HC) and divalent copper ions (Cu<sup>2+</sup>). Levofloxacin (LEV) is employed as the representative target contaminant to evaluate the system’s performance. HC has emerged as a promising technique for pollutant removal. In this study, the localized high-temperature and high-pressure conditions generated by HC not only partially activated PMS but also facilitated its interaction with Cu<sup>2+</sup> ions, leading to a pronounced synergistic enhancement in sulfate radical (SO<sub>4</sub><sup><img>−</sup>) generation and efficient pollutant degradation. Under optimized HC/Cu<sup>2+</sup>/PMS conditions (Cu<sup>2+</sup> = 5 mM, PMS = 2.5 mM, inlet pressure = 0.15 MPa, pH = 10), complete removal of LEV (30 mg/L) was achieved within 50 min. This study elucidates the degradation mechanisms and pathways of LEV within the coupled HC/Cu<sup>2+</sup>/PMS system and evaluates the ecological safety of its degradation intermediates using the U.S. EPA’s T.E.S.T. (Toxicity Estimation Software Tool). Furthermore, the system’s applicability was validated through degradation experiments involving a range of representative pollutants, demonstrating its broad-spectrum effectiveness. Crucially, the HC/Cu<sup>2+</sup>/PMS system demonstrated a superior cavitation yield (2.78 × 10<sup>−5</sup> mg/J) and a low electrical energy per order (EE/O) of 229.48 kWh/m<sup>3</sup>, highlighting its high energy efficiency and practical potential for sustainable wastewater treatment. The experimental results emphasize the system’s strong potential for the effective removal of organic pollutants from water, offering a novel and sustainable approach for advanced water treatment.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107679"},"PeriodicalIF":9.7,"publicationDate":"2025-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145531124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-12DOI: 10.1016/j.ultsonch.2025.107677
Chenchen Xu , Shouwei Wang , Yan Zhao , Jiapeng Li , Shunliang Zhang , Jing Bai , Yuxuan Shi , Xianqi Yao , Anyu Gao , Bing Zhao
The development of desirable flavor in processed lamb remains a significant challenge for the meat industry. This study aimed to investigate the effects of ultrasound-assisted vacuum tumbling (UVT) on the flavor characteristics of lamb using a combined analytical approach of flavoromics, lipidomics, and metabolomics. The results indicated that UVT significantly expedited the degradation and subsequent oxidation of crucial flavor precursors, including phospholipid metabolites (phosphatidylethanolamine, phosphatidylserine, cytidine diphosphate-diglyceride, phosphatidylcholine, and lysophosphatidylcholine), glyceride metabolites (triacylglycerols), nucleotide metabolites (inosine monophosphate and guanosine monophosphate), and amino acid metabolites (L-glutamine). This acceleration was associated with the formation of desirable flavor compounds in the marinated lamb, including Hexanal, 1-Octen-3-ol, Methional, 2-Acetylfuran, and various esters. In conclusion, this study demonstrates that UVT is a powerful strategy for intensifying and controlling the flavor profile of meat products, providing a robust theoretical foundation for the precise application of ultrasound technology in the modern meat industry.
{"title":"Unveiling the pathways of flavor formation in marinated lamb driven by ultrasound-assisted vacuum tumbling: An integrated multi-omics approach","authors":"Chenchen Xu , Shouwei Wang , Yan Zhao , Jiapeng Li , Shunliang Zhang , Jing Bai , Yuxuan Shi , Xianqi Yao , Anyu Gao , Bing Zhao","doi":"10.1016/j.ultsonch.2025.107677","DOIUrl":"10.1016/j.ultsonch.2025.107677","url":null,"abstract":"<div><div>The development of desirable flavor in processed lamb remains a significant challenge for the meat industry. This study aimed to investigate the effects of ultrasound-assisted vacuum tumbling (UVT) on the flavor characteristics of lamb using a combined analytical approach of flavoromics, lipidomics, and metabolomics. The results indicated that UVT significantly expedited the degradation and subsequent oxidation of crucial flavor precursors, including phospholipid metabolites (phosphatidylethanolamine, phosphatidylserine, cytidine diphosphate-diglyceride, phosphatidylcholine, and lysophosphatidylcholine), glyceride metabolites (triacylglycerols), nucleotide metabolites (inosine monophosphate and guanosine monophosphate), and amino acid metabolites (L-glutamine). This acceleration was associated with the formation of desirable flavor compounds in the marinated lamb, including Hexanal, 1-Octen-3-ol, Methional, 2-Acetylfuran, and various esters. In conclusion, this study demonstrates that UVT is a powerful strategy for intensifying and controlling the flavor profile of meat products, providing a robust theoretical foundation for the precise application of ultrasound technology in the modern meat industry.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107677"},"PeriodicalIF":9.7,"publicationDate":"2025-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145515824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-12DOI: 10.1016/j.ultsonch.2025.107678
Yuhan Fang , Ping Zhang , Shuang Wang , Lulu Li , Zunlai Sheng
This study innovatively employs ultrasound-assisted extraction (UAE) in conjunction with supramolecular solvents (SUPRAS) for the extraction of luteolin from peanut shells, while also investigating the potential mechanism underlying this extraction process. Initially, the formation mechanism of SUPRAS was characterized using Fourier-transform infrared spectroscopy (FTIR). Based on the results of single-factor experiments, the extraction process was optimized using response surface methodology (RSM), resulting in the determination of optimal conditions: an ultrasound temperature of 67 °C, a SUPRAS/equilibrium solution (EqS) volume ratio of 5:1, and a solid-to-liquid ratio of 1:36 g/mL. Under these conditions, the maximum luteolin yield reached 1.645 mg/g. Furthermore, through independent gradient model (IGMH) calculations, molecular orbital theory, interaction energy analysis, and molecular dynamics simulations, it was revealed that the predominant interaction force between the supramolecular solvent and the target compound is hydrogen bonding. Notably, this study also compares the SUPRAS-UAE extraction method with the traditional extraction in terms of efficiency, economic costs, and environmental impact. The results indicate that the SUPRAS-UAE technique demonstrates significant advantages in terms of its green and high-efficiency characteristics. It provides a new sustainable strategy for the efficient recovery of natural active ingredients in chemical engineering and related fields.
{"title":"Ultrasonic-Assisted extraction of luteolin from peanut shells using supramolecular solvents and its molecular mechanism","authors":"Yuhan Fang , Ping Zhang , Shuang Wang , Lulu Li , Zunlai Sheng","doi":"10.1016/j.ultsonch.2025.107678","DOIUrl":"10.1016/j.ultsonch.2025.107678","url":null,"abstract":"<div><div>This study innovatively employs ultrasound-assisted extraction (UAE) in conjunction with supramolecular solvents (SUPRAS) for the extraction of luteolin from peanut shells, while also investigating the potential mechanism underlying this extraction process. Initially, the formation mechanism of SUPRAS was characterized using Fourier-transform infrared spectroscopy (FTIR). Based on the results of single-factor experiments, the extraction process was optimized using response surface methodology (RSM), resulting in the determination of optimal conditions: an ultrasound temperature of 67 °C, a SUPRAS/equilibrium solution (EqS) volume ratio of 5:1, and a solid-to-liquid ratio of 1:36 g/mL. Under these conditions, the maximum luteolin yield reached 1.645 mg/g. Furthermore, through independent gradient model (IGMH) calculations, molecular orbital theory, interaction energy analysis, and molecular dynamics simulations, it was revealed that the predominant interaction force between the supramolecular solvent and the target compound is hydrogen bonding. Notably, this study also compares the SUPRAS-UAE extraction method with the traditional extraction in terms of efficiency, economic costs, and environmental impact. The results indicate that the SUPRAS-UAE technique demonstrates significant advantages in terms of its green and high-efficiency characteristics. It provides a new sustainable strategy for the efficient recovery of natural active ingredients in chemical engineering and related fields.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107678"},"PeriodicalIF":9.7,"publicationDate":"2025-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145509821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-11DOI: 10.1016/j.ultsonch.2025.107668
Vidit Tiwari , Subhrajit Swain , Vivek V. Ranade
Nucleation is the onset of the crystallization process, and enhancing its rate is fundamental to intensifying crystallization, be it in a batch or continuous mode of operation. The present work investigates, for the first time, the influence of hydrodynamic cavitation (HC) on enhancing the nucleation rate of paracetamol crystallization in a methanol–water system. Initially, the experiments were performed in a batch mode to determine the induction time using a vortex-based HC device (VD). The experimentally determined induction time was found to decrease with supersaturation. The use of HC was found to enhance nucleation and significantly reduce induction time. An appropriate correlation to estimate induction time is developed. Based on these findings, the VD-based continuous nucleator using a loop configuration was developed to intensify the performance of a continuous oscillatory baffle crystallizer (COBC). The VD-based nucleator improved the yield and productivity of the COBC, significantly reducing the risk of encrustation and clogging in the bends of the COBC. The approach and results presented in the current study will provide a sound basis for harnessing VD-based hydrodynamic cavitation for enhancing nucleation and intensifying antisolvent crystallization.
{"title":"Enhancing Nucleation using a Vortex-based Hydrodynamic Cavitation Device: Application to Antisolvent Crystallization of Paracetamol – Methanol – Water System","authors":"Vidit Tiwari , Subhrajit Swain , Vivek V. Ranade","doi":"10.1016/j.ultsonch.2025.107668","DOIUrl":"10.1016/j.ultsonch.2025.107668","url":null,"abstract":"<div><div>Nucleation is the onset of the crystallization process, and enhancing its rate is fundamental to intensifying crystallization, be it in a batch or continuous mode of operation. The present work investigates, for the first time, the influence of hydrodynamic cavitation (HC) on enhancing the nucleation rate of paracetamol crystallization in a methanol–water system. Initially, the experiments were performed in a batch mode to determine the induction time using a vortex-based HC device (VD). The experimentally determined induction time was found to decrease with supersaturation. The use of HC was found to enhance nucleation and significantly reduce induction time. An appropriate correlation to estimate induction time is developed. Based on these findings, the VD-based continuous nucleator using a loop configuration was developed to intensify the performance of a continuous oscillatory baffle crystallizer (COBC). The VD-based nucleator improved the yield and productivity of the COBC, significantly reducing the risk of encrustation and clogging in the bends of the COBC. The approach and results presented in the current study will provide a sound basis for harnessing VD-based hydrodynamic cavitation for enhancing nucleation and intensifying antisolvent crystallization.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107668"},"PeriodicalIF":9.7,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145509824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-11DOI: 10.1016/j.ultsonch.2025.107674
Houhong Wang , Chun Luo , Wenli Chen , Weilin Wang , Yongyun Chen , Kelei Shang , Yan Peng , Zhongmin Li
Catheter-associated urinary tract infections (CAUTIs) pose a persistent clinical challenge due to bacterial colonization and biofilm formation on indwelling urological devices. This study investigates the efficacy of Cu/Ag/Zn trimetallic nanocomposites (TMNCs) as antimicrobial coatings for urinary catheters, synthesized via a green, ultrasonic–autoclave-assisted method. The nanocomposites were thoroughly characterized using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), Brunauer–Emmett–Teller (BET) surface area analysis, and field emission scanning electron microscopy (FESEM) to confirm their mesoporous, crystalline, uniformly distributed, and thermally stable nanoparticles. An optimized ultrasonic embedding technique was employed to uniformly coat TMNCs onto commercial urinary catheters, ensuring strong adhesion and consistent coverage. Antibacterial assays demonstrated concentration-dependent inhibition zones, reaching up to 21.5 mm for Escherichia coli and 24.0 mm for Staphylococcus aureus. Minimum inhibitory concentration (MIC) tests confirmed potent bactericidal activity, with MIC values of 32 µg/mL for E. coli and 64 µg/mL for S. aureus. Anti-biofilm assessments revealed that TMNCs matched or outperformed the efficacy of vancomycin, achieving up to 86.12 % inhibition for S. aureus and 73.59 % for E. coli at 2 × MIC. Cytotoxicity testing using U87 glioblastoma cells indicated good biocompatibility, with over 79 % cell viability at 0.1 mg/mL, followed by a dose-dependent decline at higher concentrations. Long-term stability studies conducted over 60 days under varied storage conditions confirmed the photothermal and colloidal stability of the TMNCs. In conclusion, Cu/Ag/Zn TMNC-coated urinary catheters demonstrated excellent antibacterial and anti-biofilm properties, biocompatibility, and long-term stability. These multi-functional coatings present a promising strategy for reducing CAUTI incidence and improving patient safety and clinical outcomes in urological applications.
{"title":"Ultrasonically synthesized Cu/Ag/Zn nanocomposite coatings for antibacterial and antibiofilm applications in urological devices","authors":"Houhong Wang , Chun Luo , Wenli Chen , Weilin Wang , Yongyun Chen , Kelei Shang , Yan Peng , Zhongmin Li","doi":"10.1016/j.ultsonch.2025.107674","DOIUrl":"10.1016/j.ultsonch.2025.107674","url":null,"abstract":"<div><div>Catheter-associated urinary tract infections (CAUTIs) pose a persistent clinical challenge due to bacterial colonization and biofilm formation on indwelling urological devices. This study investigates the efficacy of Cu/Ag/Zn trimetallic nanocomposites (TMNCs) as antimicrobial coatings for urinary catheters, synthesized via a green, ultrasonic–autoclave-assisted method. The nanocomposites were thoroughly characterized using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), Brunauer–Emmett–Teller (BET) surface area analysis, and field emission scanning electron microscopy (FESEM) to confirm their mesoporous, crystalline, uniformly distributed, and thermally stable nanoparticles. An optimized ultrasonic embedding technique was employed to uniformly coat TMNCs onto commercial urinary catheters, ensuring strong adhesion and consistent coverage. Antibacterial assays demonstrated concentration-dependent inhibition zones, reaching up to 21.5 mm for <em>Escherichia coli</em> and 24.0 mm for <em>Staphylococcus aureus</em>. Minimum inhibitory concentration (MIC) tests confirmed potent bactericidal activity, with MIC values of 32 µg/mL for <em>E. coli</em> and 64 µg/mL for <em>S. aureus</em>. Anti-biofilm assessments revealed that TMNCs matched or outperformed the efficacy of vancomycin, achieving up to 86.12 % inhibition for <em>S. aureus</em> and 73.59 % for <em>E. coli</em> at 2 × MIC. Cytotoxicity testing using U87 glioblastoma cells indicated good biocompatibility, with over 79 % cell viability at 0.1 mg/mL, followed by a dose-dependent decline at higher concentrations. Long-term stability studies conducted over 60 days under varied storage conditions confirmed the photothermal and colloidal stability of the TMNCs. In conclusion, Cu/Ag/Zn TMNC-coated urinary catheters demonstrated excellent antibacterial and anti-biofilm properties, biocompatibility, and long-term stability. These multi-functional coatings present a promising strategy for reducing CAUTI incidence and improving patient safety and clinical outcomes in urological applications.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107674"},"PeriodicalIF":9.7,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145499216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-11DOI: 10.1016/j.ultsonch.2025.107675
Hoang Duy Huynh , Parushi Nargotra , Hui-Min David Wang , Yung-Hsiang Tsai , Chien-Chih Chiu , Chwen-Jen Shieh , Yung-Chuan Liu , Chia-Hung Kuo
Strawberry guava leaves (Psidium cattleianum) are an attractive phenolic-rich byproduct for food and pharmaceutical production, with potential benefits for human health due to their exceptional antioxidant activity among plant-based resources. However, there are no systematic studies on sonochemical phenolic extraction from this material. Therefore, the goal of this study was to unveil the mechanism of ultrasound-assisted extraction (UAE) for phenolic recovery from Psidium cattleianum leaves. Pseudo-second-order kinetic modeling, Biot number, and thermodynamic analysis were introduced to describe the extraction mechanisms and then kinetic parameters such as saturation concentration (Cs), extraction rate (k), diffusion coefficient (Deff), and mass transfer coefficient (KT) compared with conventional shaking extraction (CSE) to establish the process intensification benefits. The result of pseudo-second-order kinetic analysis revealed a biphasic extraction mechanism consisting of initial rapid release, followed by diffusion-controlled mass transfer, with internal mass transfer identified as the rate-limiting step (Bi > 50). The UAE process was spontaneous (ΔGo < 0), endothermic (ΔHo = 3.8151 kJ/mol), and entropy-driven (ΔSo > 0). The presence of ultrasound technology enabled UAE to demonstrate superior performance compared to CSE, which significantly increased Cs, k, De and KT. Taken together, the integration of kinetic and thermodynamic analyses offers a robust framework for understanding sonochemical processes and fundamental insights into the nature of UAE from Psidium cattleianum leaves, thereby overcoming scale-up challenges and facilitating industrial applications.
{"title":"Unveiling the mechanism of ultrasound-assisted phenolic extraction from Psidium cattleianum leaves: Kinetic, mass transfer, and thermodynamic insights","authors":"Hoang Duy Huynh , Parushi Nargotra , Hui-Min David Wang , Yung-Hsiang Tsai , Chien-Chih Chiu , Chwen-Jen Shieh , Yung-Chuan Liu , Chia-Hung Kuo","doi":"10.1016/j.ultsonch.2025.107675","DOIUrl":"10.1016/j.ultsonch.2025.107675","url":null,"abstract":"<div><div>Strawberry guava leaves (<em>Psidium cattleianum</em>) are an attractive phenolic-rich byproduct for food and pharmaceutical production, with potential benefits for human health due to their exceptional antioxidant activity among plant-based resources. However, there are no systematic studies on sonochemical phenolic extraction from this material. Therefore, the goal of this study was to unveil the mechanism of ultrasound-assisted extraction (UAE) for phenolic recovery from <em>Psidium cattleianum</em> leaves. Pseudo-second-order kinetic modeling, Biot number, and thermodynamic analysis were introduced to describe the extraction mechanisms and then kinetic parameters such as saturation concentration (<em>C<sub>s</sub></em>), extraction rate (<em>k)</em>, diffusion coefficient (<em>D<sub>eff</sub></em>), and mass transfer coefficient (<em>K<sub>T</sub></em>) compared with conventional shaking extraction (CSE) to establish the process intensification benefits. The result of pseudo-second-order kinetic analysis revealed a biphasic extraction mechanism consisting of initial rapid release, followed by diffusion-controlled mass transfer, with internal mass transfer identified as the rate-limiting step (<em>Bi</em> > 50). The UAE process was spontaneous (ΔG<sup>o</sup> < 0), endothermic (ΔH<sup>o</sup> = 3.8151 kJ/mol), and entropy-driven (ΔS<sup>o</sup> > 0). The presence of ultrasound technology enabled UAE to demonstrate superior performance compared to CSE, which significantly increased <em>C<sub>s</sub></em>, <em>k, D<sub>e</sub></em> and <em>K<sub>T</sub></em>. Taken together, the integration of kinetic and thermodynamic analyses offers a robust framework for understanding sonochemical processes and fundamental insights into the nature of UAE from <em>Psidium cattleianum</em> leaves, thereby overcoming scale-up challenges and facilitating industrial applications.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107675"},"PeriodicalIF":9.7,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145509822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Conjugates of kidney bean protein isolate (KBPI) and oat β-glucan (OG) were successfully prepared via ultrasonic-assisted Maillard reaction (wet heating). The effects of ultrasonic power density (9.62–29.37 W/cm2) on its structural characteristics, in vitro digestive behavior, and emulsifying properties were investigated. The results demonstrated that ultrasonic treatment significantly enhanced the covalent conjugation between KBPI and OG. Specifically, the ultrasonically modified conjugate (UHKBPI-OG3) prepared at 22.42 W/cm2 exhibited higher grafting degree of 23.53 %. Appropriate ultrasonic treatment reduced the average particle size and polydispersity index (PDI) of conjugates, as well as increasing the absolute value of their ζ-potential. Circular dichroism (CD) analysis revealed that ultrasound-assisted wet heating induced conformational changes in KBPI, showing a significant reduction in α-helix content accompanied by an increase in random coil structure. This structural transition suggests enhanced molecular flexibility, which is favorable for achieving higher glycosylation efficiency. Ultrasonic treatment enhanced the emulsifying properties of UHKBPI-OG3, increasing emulsifying activity index (EAI) and emulsion stability index (ESI) by 20.75 % and 15.65 %, respectively, compared to wet heating. Concurrent reductions in interfacial tension, apparent viscosity, and droplet size further contributed to improved emulsion stability. Furthermore, UHKBPI-OG3 conjugate (24.22 W/cm2) exhibited the lowest protein digestibility. These findings demonstrate that optimized ultrasonic power density enhances KBPI-OG covalent conjugation. The improved functional properties of conjugates, achieved through modulated protein-polysaccharide interactions, provide theoretical support for developing novel emulsion stabilizers.
{"title":"Effect of ultrasound treatment on kidney bean protein isolate-oat β-glucan glycation conjugates: The structure, digestibility and emulsifying properties","authors":"Zhigang Sun , Chaojiang Dong , Yue Gu , Yiying Jin , Fangxiao Lou , Tianfu Cheng , Lingling Zhang , Zengwang Guo","doi":"10.1016/j.ultsonch.2025.107676","DOIUrl":"10.1016/j.ultsonch.2025.107676","url":null,"abstract":"<div><div>Conjugates of kidney bean protein isolate (KBPI) and oat β-glucan (OG) were successfully prepared via ultrasonic-assisted Maillard reaction (wet heating). The effects of ultrasonic power density (9.62–29.37 W/cm<sup>2</sup>) on its structural characteristics, <em>in vitro</em> digestive behavior, and emulsifying properties were investigated. The results demonstrated that ultrasonic treatment significantly enhanced the covalent conjugation between KBPI and OG. Specifically, the ultrasonically modified conjugate (UHKBPI-OG3) prepared at 22.42 W/cm<sup>2</sup> exhibited higher grafting degree of 23.53 %. Appropriate ultrasonic treatment reduced the average particle size and polydispersity index (PDI) of conjugates, as well as increasing the absolute value of their ζ-potential. Circular dichroism (CD) analysis revealed that ultrasound-assisted wet heating induced conformational changes in KBPI, showing a significant reduction in α-helix content accompanied by an increase in random coil structure. This structural transition suggests enhanced molecular flexibility, which is favorable for achieving higher glycosylation efficiency<em>.</em> Ultrasonic treatment enhanced the emulsifying properties of UHKBPI-OG3, increasing emulsifying activity index (EAI) and emulsion stability index (ESI) by 20.75 % and 15.65 %, respectively, compared to wet heating. Concurrent reductions in interfacial tension, apparent viscosity, and droplet size further contributed to improved emulsion stability. Furthermore, UHKBPI-OG3 conjugate (24.22 W/cm<sup>2</sup>) exhibited the lowest protein digestibility. These findings demonstrate that optimized ultrasonic power density enhances KBPI-OG covalent conjugation. The improved functional properties of conjugates, achieved through modulated protein-polysaccharide interactions, provide theoretical support for developing novel emulsion stabilizers.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107676"},"PeriodicalIF":9.7,"publicationDate":"2025-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145509823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-11-09DOI: 10.1016/j.ultsonch.2025.107673
Li-Qiang Zhao , Zhuo-Qiong Li , Yue-Fan Liu , Meng-Ting Jiang , Ya-Nan Liu , Xin-Lan Zhang , Yu-Jie Sun , Jia-Lun Duan , Chun-Jie Bao , Jin-Ao Duan
Curcumin is a natural bioactive compound with a wide range of established health benefits. However, its practical applications are severely limited due to extremely poor aqueous solubility, which directly leads to low bioavailability. While polysaccharides like Lycium barbarum polysaccharide (LBP) can partially improve curcumin solubility, their solubilization efficiency remains limited. To overcome this challenge, we implemented ultrasonication as an effective processing strategy to enhance LBP’s capacity to promote curcumin dissolution. Our findings show that ultrasound-induced cavitation and related physico-chemical effects markedly improve LBP’s solubilization performance. The ultrasonically-assisted curcumin-LBP complex (CL-U) was systematically optimized through response surface methodology (RSM), identifying ultrasonic power, duration, and temperature as critical parameters. Extensive characterization verified that ultrasonication is essential for producing spherical core–shell nanoparticles, achieving a 2.23-fold enhancement in drug loading efficiency along with superior colloidal stability. Additional evidence from FTIR spectroscopy and acid hydrolysis experiments confirmed that ultrasonication reinforces hydrogen bonding as the principal intermolecular interaction stabilizing the complex. Biologically, CL-U demonstrated rapid cellular uptake in 4T1 cells within one hour and showed substantially improved antioxidant performance in both DPPH and ABTS assays. These functional gains are directly linked to the ultrasound-mediated improvements in solubility, stability, and bioavailability. This research establishes ultrasonication as a crucial sonochemical approach for constructing advanced polysaccharide-based delivery systems, providing a viable pathway for curcumin utilization in functional foods and pharmaceutical products.
{"title":"Ultrasonic complexation with Lycium barbarum polysaccharide significantly enhances the aqueous solubility and bioavailability of curcumin","authors":"Li-Qiang Zhao , Zhuo-Qiong Li , Yue-Fan Liu , Meng-Ting Jiang , Ya-Nan Liu , Xin-Lan Zhang , Yu-Jie Sun , Jia-Lun Duan , Chun-Jie Bao , Jin-Ao Duan","doi":"10.1016/j.ultsonch.2025.107673","DOIUrl":"10.1016/j.ultsonch.2025.107673","url":null,"abstract":"<div><div>Curcumin is a natural bioactive compound with a wide range of established health benefits. However, its practical applications are severely limited due to extremely poor aqueous solubility, which directly leads to low bioavailability. While polysaccharides like <em>Lycium barbarum</em> polysaccharide (LBP) can partially improve curcumin solubility, their solubilization efficiency remains limited. To overcome this challenge, we implemented ultrasonication as an effective processing strategy to enhance LBP’s capacity to promote curcumin dissolution. Our findings show that ultrasound-induced cavitation and related physico-chemical effects markedly improve LBP’s solubilization performance. The ultrasonically-assisted curcumin-LBP complex (CL-U) was systematically optimized through response surface methodology (RSM), identifying ultrasonic power, duration, and temperature as critical parameters. Extensive characterization verified that ultrasonication is essential for producing spherical core–shell nanoparticles, achieving a 2.23-fold enhancement in drug loading efficiency along with superior colloidal stability. Additional evidence from FTIR spectroscopy and acid hydrolysis experiments confirmed that ultrasonication reinforces hydrogen bonding as the principal intermolecular interaction stabilizing the complex. Biologically, CL-U demonstrated rapid cellular uptake in 4T1 cells within one hour and showed substantially improved antioxidant performance in both DPPH and ABTS assays. These functional gains are directly linked to the ultrasound-mediated improvements in solubility, stability, and bioavailability. This research establishes ultrasonication as a crucial sonochemical approach for constructing advanced polysaccharide-based delivery systems, providing a viable pathway for curcumin utilization in functional foods and pharmaceutical products.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"123 ","pages":"Article 107673"},"PeriodicalIF":9.7,"publicationDate":"2025-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号