Pub Date : 2025-02-10DOI: 10.1016/j.watres.2025.123263
Zeyang Zhao, Jingyi Yuan, Qiuda Zheng, Benjamin J. Tscharke, Tim Boogaerts, Zhe Wang, Shuo Chen, Jake W. O'Brien, Alexander L.N. van Nuijs, Adrian Covaci, Jochen Mueller, Phong K. Thai
Monitoring antidepressant use is important for understanding mental health status in populations and detecting potential misuse. Wastewater-based epidemiology (WBE) is a cost-effective approach to conduct health monitoring but requires valid correction factors (CFs) to accurately convert wastewater per capita mass loads into consumption estimates. Most existing CFs are calculated from pharmacokinetic studies with small cohorts and are not specifically validated for WBE purposes. This study aimed to fill this knowledge gap by calibrating and validating the CFs for 5 commonly prescribed antidepressants. CFs were calibrated by dividing corresponding geo-located sales data by wastewater mass loads from 18 wastewater treatment plants in Australia for the same 3.5-year period. The refined CFs were 9.0 for fluoxetine, 6.4 for venlafaxine, and 25 for quetiapine. For the case of racemic citalopram and the pure S-enantiomer (escitalopram), individual CFs were proposed as 2.0 and 11, respectively. To validate their applicability, the new CFs were applied to independent datasets of wastewater samples collected in Belgium (2019 to 2022) and Australia (2020) and compared with sales data. The new calibrated CFs produced more accurate wastewater-based estimates of consumption for citalopram, escitalopram, fluoxetine, venlafaxine, and quetiapine, enhancing the capability of WBE in public health surveillance.
{"title":"Utilizing national wastewater and sales data to derive and validate the correction factors of five common antidepressants for wastewater-based epidemiology","authors":"Zeyang Zhao, Jingyi Yuan, Qiuda Zheng, Benjamin J. Tscharke, Tim Boogaerts, Zhe Wang, Shuo Chen, Jake W. O'Brien, Alexander L.N. van Nuijs, Adrian Covaci, Jochen Mueller, Phong K. Thai","doi":"10.1016/j.watres.2025.123263","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123263","url":null,"abstract":"Monitoring antidepressant use is important for understanding mental health status in populations and detecting potential misuse. Wastewater-based epidemiology (WBE) is a cost-effective approach to conduct health monitoring but requires valid correction factors (CFs) to accurately convert wastewater per capita mass loads into consumption estimates. Most existing CFs are calculated from pharmacokinetic studies with small cohorts and are not specifically validated for WBE purposes. This study aimed to fill this knowledge gap by calibrating and validating the CFs for 5 commonly prescribed antidepressants. CFs were calibrated by dividing corresponding geo-located sales data by wastewater mass loads from 18 wastewater treatment plants in Australia for the same 3.5-year period. The refined CFs were 9.0 for fluoxetine, 6.4 for venlafaxine, and 25 for quetiapine. For the case of racemic citalopram and the pure S-enantiomer (escitalopram), individual CFs were proposed as 2.0 and 11, respectively. To validate their applicability, the new CFs were applied to independent datasets of wastewater samples collected in Belgium (2019 to 2022) and Australia (2020) and compared with sales data. The new calibrated CFs produced more accurate wastewater-based estimates of consumption for citalopram, escitalopram, fluoxetine, venlafaxine, and quetiapine, enhancing the capability of WBE in public health surveillance.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"8 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-10DOI: 10.1016/j.watres.2025.123260
Ying Lu , Kejian Chu , Zulin Hua , Chang Gao , Yuanyuan Liu
Despite their widespread occurrence and significant environmental implications, the influence of sluice operations on the mobility of perfluoroalkyl acid (PFAA) in riverine sediments remains largely unexplored. To address this gap, a series of flume experiments were conducted to simulate the sedimentary migration of PFAA under the turbulent conditions generated by opening a sluice. Our study provides novel insights into the mechanisms by which plunging turbulence modulates the transfer of sedimentary PFAAs across the sediment‒water interface. Significant transient release effects were observed in the dissolved and suspended particulate matter (SPM) phases of PFAA, with total concentrations maintaining relative stability over extended periods following disturbance. The fluviraption of plunging turbulence increased PFAA concentrations in the surface sedimentary and porewater phases but weakened the adsorption performance of resuspended particles for the chemicals in the lower reach of the sluice. The instantaneous release of PFAA from sediment, fueled by turbulence, was identified as the primary driver of total mass transfer across the interface, increasing exponentially with the Reynolds number (Rex, R2=0.99, p < 0.01). Notably, the peak PFAA release flux in the SPM phase lagged behind that in the dissolved phase, underscoring the dynamic interplay between phases. A structural equation model (SEM) revealed that plunging turbulence indirectly governs the cross-interface transfer of sedimentary PFAA by altering environmental physicochemical parameters and enhancing porewater diffusion. This finding underscores the complex, coupled effects of scour behavior and physicochemical properties on PFAA fate. Our study offers a unique perspective on the dynamic mechanisms underlying PFAA multimedia migration under sluice operation, contributing valuable insights for managing and regulating these emerging contaminants in aquatic environments.
{"title":"The response of PFAA mobility in highly contaminated sediment to sluice operation: Coupled effects of scour behavior and physicochemical properties","authors":"Ying Lu , Kejian Chu , Zulin Hua , Chang Gao , Yuanyuan Liu","doi":"10.1016/j.watres.2025.123260","DOIUrl":"10.1016/j.watres.2025.123260","url":null,"abstract":"<div><div>Despite their widespread occurrence and significant environmental implications, the influence of sluice operations on the mobility of perfluoroalkyl acid (PFAA) in riverine sediments remains largely unexplored. To address this gap, a series of flume experiments were conducted to simulate the sedimentary migration of PFAA under the turbulent conditions generated by opening a sluice. Our study provides novel insights into the mechanisms by which plunging turbulence modulates the transfer of sedimentary PFAAs across the sediment‒water interface. Significant transient release effects were observed in the dissolved and suspended particulate matter (SPM) phases of PFAA, with total concentrations maintaining relative stability over extended periods following disturbance. The fluviraption of plunging turbulence increased PFAA concentrations in the surface sedimentary and porewater phases but weakened the adsorption performance of resuspended particles for the chemicals in the lower reach of the sluice. The instantaneous release of PFAA from sediment, fueled by turbulence, was identified as the primary driver of total mass transfer across the interface, increasing exponentially with the Reynolds number (Re<sub>x</sub>, R<sup>2</sup>=0.99, <em>p</em> < 0.01). Notably, the peak PFAA release flux in the SPM phase lagged behind that in the dissolved phase, underscoring the dynamic interplay between phases. A structural equation model (SEM) revealed that plunging turbulence indirectly governs the cross-interface transfer of sedimentary PFAA by altering environmental physicochemical parameters and enhancing porewater diffusion. This finding underscores the complex, coupled effects of scour behavior and physicochemical properties on PFAA fate. Our study offers a unique perspective on the dynamic mechanisms underlying PFAA multimedia migration under sluice operation, contributing valuable insights for managing and regulating these emerging contaminants in aquatic environments.</div></div>","PeriodicalId":443,"journal":{"name":"Water Research","volume":"276 ","pages":"Article 123260"},"PeriodicalIF":11.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-10DOI: 10.1016/j.watres.2025.123269
Natan Van Wichelen , Tim Boogaerts , Maarten Quireyns , Rania Dermitzaki , Peter Delputte , Noor Ul Hudda , Naomi De Roeck , Bavo Verhaegen , Koenraad Van Hoorde , Hadrien Maloux , Veronik Hutse , Celine Gys , Adrian Covaci , Alexander L.N. van Nuijs
Based on reports of ketamine seizures, self-reported consumption and harmful associated health effects, there are signs of increased ketamine use. However, monitoring population-level consumption patterns remains difficult. This study employed wastewater-based epidemiology (WBE) to address this challenge by analysing influent wastewater (IWW) from Belgium for biomarkers of ketamine consumption to evaluate spatio-temporal trends.
Daily 24-h composite IWW samples were collected from 26 locations across the northern part of Belgium (Flanders and Brussels region), every Monday and Wednesday in 2023 (n = 716). Additionally, a temporal dataset from daily IWW samples from Leuven (n = 399) and Brussels (n = 285) in 2021 and 2022 was evaluated using time series analysis. Measured concentrations of ketamine, norketamine and dehydronorketamine in IWW were transformed to population-normalised mass loads (PNML, expressed in mg/day/1000 inhabitants) to assess ketamine consumption patterns.
Ketamine, norketamine and dehydronorketamine were detected in respectively 98%, 96%, and 76% of all samples. Substantial consumption was observed in both urban and rural areas, with higher PNMLs in major cities and the east of Flanders. Temporal analysis showed no seasonal trends but an increase in weekend consumption, likely linked to recreational use. PNMLs increased 7- to 11-fold compared to available data in 2012. Actual consumption and direct disposal down the drain could be differentiated by the investigation of the ketamine to norketamine ratio.
Ketamine use is widespread across the north of Belgium, encompassing a relatively stable, year-round consumption. In future WBE studies, it is recommended to measure both norketamine and ketamine to assess ketamine consumption and to rule out any dumping events.
{"title":"Ketamine, a new (or old) kid on the block: A comprehensive three-year spatio-temporal study in Belgium through wastewater-based epidemiology","authors":"Natan Van Wichelen , Tim Boogaerts , Maarten Quireyns , Rania Dermitzaki , Peter Delputte , Noor Ul Hudda , Naomi De Roeck , Bavo Verhaegen , Koenraad Van Hoorde , Hadrien Maloux , Veronik Hutse , Celine Gys , Adrian Covaci , Alexander L.N. van Nuijs","doi":"10.1016/j.watres.2025.123269","DOIUrl":"10.1016/j.watres.2025.123269","url":null,"abstract":"<div><div>Based on reports of ketamine seizures, self-reported consumption and harmful associated health effects, there are signs of increased ketamine use. However, monitoring population-level consumption patterns remains difficult. This study employed wastewater-based epidemiology (WBE) to address this challenge by analysing influent wastewater (IWW) from Belgium for biomarkers of ketamine consumption to evaluate spatio-temporal trends.</div><div>Daily 24-h composite IWW samples were collected from 26 locations across the northern part of Belgium (Flanders and Brussels region), every Monday and Wednesday in 2023 (<em>n</em> = 716). Additionally, a temporal dataset from daily IWW samples from Leuven (<em>n</em> = 399) and Brussels (<em>n</em> = 285) in 2021 and 2022 was evaluated using time series analysis. Measured concentrations of ketamine, norketamine and dehydronorketamine in IWW were transformed to population-normalised mass loads (PNML, expressed in mg/day/1000 inhabitants) to assess ketamine consumption patterns.</div><div>Ketamine, norketamine and dehydronorketamine were detected in respectively 98%, 96%, and 76% of all samples. Substantial consumption was observed in both urban and rural areas, with higher PNMLs in major cities and the east of Flanders. Temporal analysis showed no seasonal trends but an increase in weekend consumption, likely linked to recreational use. PNMLs increased 7- to 11-fold compared to available data in 2012. Actual consumption and direct disposal down the drain could be differentiated by the investigation of the ketamine to norketamine ratio.</div><div>Ketamine use is widespread across the north of Belgium, encompassing a relatively stable, year-round consumption. In future WBE studies, it is recommended to measure both norketamine and ketamine to assess ketamine consumption and to rule out any dumping events.</div></div>","PeriodicalId":443,"journal":{"name":"Water Research","volume":"276 ","pages":"Article 123269"},"PeriodicalIF":11.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-10DOI: 10.1016/j.watres.2025.123268
Ma Zi Jun , Tian C. Zhang , Tian Li
{"title":"Response to the commentary on the article 'Fluid energy theory of membrane' in water research","authors":"Ma Zi Jun , Tian C. Zhang , Tian Li","doi":"10.1016/j.watres.2025.123268","DOIUrl":"10.1016/j.watres.2025.123268","url":null,"abstract":"","PeriodicalId":443,"journal":{"name":"Water Research","volume":"276 ","pages":"Article 123268"},"PeriodicalIF":11.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-10DOI: 10.1016/j.watres.2025.123267
Hongyun Niu , Shaojie Shi , Siyu Zhu , Yuling He , Qiwen An , Hao Ding , Xuwenqi Zhang , Dongbin Wei , Yali Shi , Yaqi Cai
Activation of oxygen by zero-valent iron (ZVI) to in-situ produce reactive oxidant species (ROS) provides a promising low-carbon and “green” technology for water purification. However, poor ROS yields and easy inactivation limit its engineering application for organic pollutants elimination. Herein, we fabricated a novel Fe-based catalyst with Fe(II)-regenerative surface derived from phosphatized sewage sludge and iron salts. The achieved materials were composed of sludge biochars, FexP, Fe, and FexC (SL-FexP@Fe-FexC) and possessed core/shell structure. SL-FexP@Fe-FexC showed high efficiency in degrading recalcitrant organic pollutants 3,4,5,6-tetrachloropicolinic acid (TCPA) from water at pH 3–10 or in different salts solution without the need of exogenous H2O2. When sludge was pretreated with 1.0 M H3PO4 and then soaked in 50 mM FeCl3 solution before carbonization, the obtained SL1.0M-FexP@Fe-FexC50mM could degrade TCPA with almost 100 % efficiency in ten consecutive recycle runs. This material demonstrates better activity persistence than most of the reported Fe-based catalysts. The EPR and quenching tests indicated that O2•- radicals generated from Fe(II)/O2 reaction were the main active species for TCPA degradation. The electrochemical experiments revealed that strong affinity of O2 and fast electron transfer from inner Fe/FexC to SL-FexP shell improved the yields of O2•- and regeneration of Fe(II) species.
{"title":"Superoxide anion radicals mediated degradation of tetrachloropicolinic acid in biochars-FexP@Fe-FexC/O2 system with excellent reactivity durability","authors":"Hongyun Niu , Shaojie Shi , Siyu Zhu , Yuling He , Qiwen An , Hao Ding , Xuwenqi Zhang , Dongbin Wei , Yali Shi , Yaqi Cai","doi":"10.1016/j.watres.2025.123267","DOIUrl":"10.1016/j.watres.2025.123267","url":null,"abstract":"<div><div>Activation of oxygen by zero-valent iron (ZVI) to in-situ produce reactive oxidant species (ROS) provides a promising low-carbon and “green” technology for water purification. However, poor ROS yields and easy inactivation limit its engineering application for organic pollutants elimination. Herein, we fabricated a novel Fe-based catalyst with Fe(II)-regenerative surface derived from phosphatized sewage sludge and iron salts. The achieved materials were composed of sludge biochars, Fe<em><sub>x</sub></em>P, Fe, and Fe<em><sub>x</sub></em>C (SL-Fe<em><sub>x</sub></em>P@Fe-Fe<em><sub>x</sub></em>C) and possessed core/shell structure. SL-Fe<em><sub>x</sub></em>P@Fe-Fe<em><sub>x</sub></em>C showed high efficiency in degrading recalcitrant organic pollutants 3,4,5,6-tetrachloropicolinic acid (TCPA) from water at pH 3–10 or in different salts solution without the need of exogenous H<sub>2</sub>O<sub>2</sub>. When sludge was pretreated with 1.0 M H<sub>3</sub>PO<sub>4</sub> and then soaked in 50 mM FeCl<sub>3</sub> solution before carbonization, the obtained SL<sub>1.0M</sub>-Fe<em><sub>x</sub></em>P@Fe-Fe<em><sub>x</sub></em>C<sub>50</sub> <sub>mM</sub> could degrade TCPA with almost 100 % efficiency in ten consecutive recycle runs. This material demonstrates better activity persistence than most of the reported Fe-based catalysts. The EPR and quenching tests indicated that O<sub>2</sub><sup>•-</sup> radicals generated from Fe(II)/O<sub>2</sub> reaction were the main active species for TCPA degradation. The electrochemical experiments revealed that strong affinity of O<sub>2</sub> and fast electron transfer from inner Fe/Fe<em><sub>x</sub></em>C to SL-Fe<em><sub>x</sub></em>P shell improved the yields of O<sub>2</sub><sup>•-</sup> and regeneration of Fe(II) species.</div></div>","PeriodicalId":443,"journal":{"name":"Water Research","volume":"276 ","pages":"Article 123267"},"PeriodicalIF":11.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Understanding how synthetic microbial community (SMC) respond to environmental disturbances is the key to realizing SMC engineering applications. Here, dibutyl phthalate (DBP) and levofloxacin (LOFX) were used as environmental disturbances to study their effects on the aerobic denitrification functional stability of SMC composed of Pseudomonas aeruginosa N2 (PA), Acinetobacter baumannii N1(AC) and Aeromonas hydrophila (AH). The results showed that aerobic denitrification efficiency could be maintained at about 93% under DBP or LOFX disturbance, and interspecific communication was mainly carried out through N-butyryl-L-homoserine lactone (C4-HSL) and N-(3-oxododecanoyl)-L-homoserine lactone (3OC12-HSL), correspondingly. DBP and LOFX induced the acceleration of tricarboxylic acid (TCA) cycle, which facilitated the energy flux and extracellular polymeric substances (EPS) production, thereby allowing SMC to adapt to disturbances. Under DBP disturbance, DBP stimulated phenazine-1-carboxylic acid production to accelerate electron transfer from the quinone pool to complex III, resulting in an increase in electron transfer activity. Up-regulation of complex I, complex III and heme synthesis genes under LOFX disturbance led to enhanced denitrification enzymes expression and electron transfer efficiency. SMC re-regulated different metabolic pathways to build metabolic networks to maintain normal metabolic activity under different disturbances. Overall, SMC maintained functional stability through the labor division in modulation of interspecific communication, formation of defensive barriers, promotion of energy flux, directional transfer of electron flux, and reconstruction of metabolic networks. DBP stimulated AH and PA to occupy functional dominance, while LOFX induced AC and PA to play a major role. The understanding of the stability mechanism under different environmental disturbances provides valuable guidance for stability maintenance and engineering applications of SMC.
{"title":"Synthetic microbial community maintains the functional stability of aerobic denitrification under environmental disturbance: Insight into the mechanism of interspecific division of labor","authors":"Yue He, Hui Yun, Liang Peng, Wenxue Wang, Ting Xu, Wenjie Zhang, Xiangkai Li","doi":"10.1016/j.watres.2025.123270","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123270","url":null,"abstract":"Understanding how synthetic microbial community (SMC) respond to environmental disturbances is the key to realizing SMC engineering applications. Here, dibutyl phthalate (DBP) and levofloxacin (LOFX) were used as environmental disturbances to study their effects on the aerobic denitrification functional stability of SMC composed of <em>Pseudomonas aeruginosa</em> N2 (PA), <em>Acinetobacter baumannii</em> N1(AC) and <em>Aeromonas hydrophila</em> (AH). The results showed that aerobic denitrification efficiency could be maintained at about 93% under DBP or LOFX disturbance, and interspecific communication was mainly carried out through N-butyryl-L-homoserine lactone (C<sub>4</sub>-HSL) and N-(3-oxododecanoyl)-L-homoserine lactone (3OC<sub>12</sub>-HSL), correspondingly. DBP and LOFX induced the acceleration of tricarboxylic acid (TCA) cycle, which facilitated the energy flux and extracellular polymeric substances (EPS) production, thereby allowing SMC to adapt to disturbances. Under DBP disturbance, DBP stimulated phenazine-1-carboxylic acid production to accelerate electron transfer from the quinone pool to complex III, resulting in an increase in electron transfer activity. Up-regulation of complex I, complex III and heme synthesis genes under LOFX disturbance led to enhanced denitrification enzymes expression and electron transfer efficiency. SMC re-regulated different metabolic pathways to build metabolic networks to maintain normal metabolic activity under different disturbances. Overall, SMC maintained functional stability through the labor division in modulation of interspecific communication, formation of defensive barriers, promotion of energy flux, directional transfer of electron flux, and reconstruction of metabolic networks. DBP stimulated AH and PA to occupy functional dominance, while LOFX induced AC and PA to play a major role. The understanding of the stability mechanism under different environmental disturbances provides valuable guidance for stability maintenance and engineering applications of SMC.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"78 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143385793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-09DOI: 10.1016/j.watres.2025.123257
Chao Chang , Mi Ren , Han Wang , Sisi Ye , Xiaofeng Tang , Ding He , En Hu , Ming Li , Baozhu Pan
Revealing the spatial variation of aquatic invertebrates and their response to biotic and abiotic factors, from headwaters to estuaries, is crucial for understanding their successional patterns and protecting watershed ecosystems. This study aimed to explore the biogeographic patterns and identify the primary drivers of invertebrate community structure across river networks of varying sizes using environmental DNA (eDNA) technology. To assess the contribution of biotic and abiotic factors to invertebrate communities, we collected six categories of abiotic factors: geography, climate, hydro-morphology, human footprint index, land use, and water quality. For biotic factors, four microbial groups including archaea, bacteria, fungi, and protists were identified using eDNA techniques. Water samples were collected from a total of 187 sample sites in the upper Hanjiang River basin (China) during two seasons (Spring and Autumn), covering the transition from the headwater tributaries to the lower reaches of the main channel. The results revealed that environmental factors explained approximately 6.5 times more variation in invertebrate eDNA communities than geographic factors. Water quality and biotic factors had strong explanatory power for invertebrate eDNA diversity. Ecological succession of invertebrate eDNA communities along the river continuum showed a shift from Arthropoda-dominated communities in the headwaters to a co-dominance of Arthropoda, Rotifera, and Cnidaria downstream. The cumulative dendritic distance upstream, representing the location of each sampling site within the river network, emerged as the most predictive spatial feature. Significant differences were observed in the dominant environmental factors influencing community diversity across different river network sizes. In small river networks, invertebrate eDNA diversity was primarily influenced by biotic factors, while in medium-sized networks, it was shaped by a combination of biotic factors and water quality. In large river networks, water quality emerged as the primary driver. These findings suggest that invertebrate communities throughout the Hanjiang River basin undergo ecological succession along the river continuum, primarily shaped by environmental factors related to river network size.
{"title":"Riverine network size determined major driving factors of the composition and diversity of aquatic invertebrate communities in a multi-tributary mountain river basin","authors":"Chao Chang , Mi Ren , Han Wang , Sisi Ye , Xiaofeng Tang , Ding He , En Hu , Ming Li , Baozhu Pan","doi":"10.1016/j.watres.2025.123257","DOIUrl":"10.1016/j.watres.2025.123257","url":null,"abstract":"<div><div>Revealing the spatial variation of aquatic invertebrates and their response to biotic and abiotic factors, from headwaters to estuaries, is crucial for understanding their successional patterns and protecting watershed ecosystems. This study aimed to explore the biogeographic patterns and identify the primary drivers of invertebrate community structure across river networks of varying sizes using environmental DNA (eDNA) technology. To assess the contribution of biotic and abiotic factors to invertebrate communities, we collected six categories of abiotic factors: geography, climate, hydro-morphology, human footprint index, land use, and water quality. For biotic factors, four microbial groups including archaea, bacteria, fungi, and protists were identified using eDNA techniques. Water samples were collected from a total of 187 sample sites in the upper Hanjiang River basin (China) during two seasons (Spring and Autumn), covering the transition from the headwater tributaries to the lower reaches of the main channel. The results revealed that environmental factors explained approximately 6.5 times more variation in invertebrate eDNA communities than geographic factors. Water quality and biotic factors had strong explanatory power for invertebrate eDNA diversity. Ecological succession of invertebrate eDNA communities along the river continuum showed a shift from Arthropoda-dominated communities in the headwaters to a co-dominance of Arthropoda, Rotifera, and Cnidaria downstream. The cumulative dendritic distance upstream, representing the location of each sampling site within the river network, emerged as the most predictive spatial feature. Significant differences were observed in the dominant environmental factors influencing community diversity across different river network sizes. In small river networks, invertebrate eDNA diversity was primarily influenced by biotic factors, while in medium-sized networks, it was shaped by a combination of biotic factors and water quality. In large river networks, water quality emerged as the primary driver. These findings suggest that invertebrate communities throughout the Hanjiang River basin undergo ecological succession along the river continuum, primarily shaped by environmental factors related to river network size.</div></div>","PeriodicalId":443,"journal":{"name":"Water Research","volume":"276 ","pages":"Article 123257"},"PeriodicalIF":11.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Understanding the impacts of environmentally relevant low-level antibiotics on aquatic microbiomes and resistomes is crucial for risk assessment of anthropogenic antibiotic contamination. Here, we investigated the effects of seven subinhibitory concentrations of trimethoprim and lincomycin (10 ng/L to 10 mg/L), individually and in combination, on surface water microcosms, using unspiked samples as controls. Metagenomic sequencing revealed a decrease in bacterial community α-diversity and an increase in resistome α-diversity with rising antibiotic concentrations. Notably, the β-diversity of both bacterial communities and resistomes exhibited a biphasic response, decreasing and then increasing with breakpoint concentrations of 2.73 μg/L and 0.68 μg/L, respectively. We also observed concentration-dependent increases in certain metagenome-assembled antibiotic-resistant bacteria (MAARB) and antibiotic resistance genes (ARGs), with minimum selective concentrations (MSCs) of 2.28 μg/L for trimethoprim targeting OXA-21 and 32.4 μg/L for lincomycin targeting erm(F). Among various metrics for identifying risk thresholds that induce significant changes in microbial taxa, resistomes, individual ARGs, and MAARB, the breakpoint concentration derived from resistome β-diversity was the most conservative. We propose integrating this metric into environmental risk assessment frameworks for antibiotics. Our study provides a systematic evaluation of antibiotic impacts on aquatic microbiomes and resistomes, offering key insights for refining risk assessments of antibiotic contamination in aquatic environments.
{"title":"Comprehensive assessment of antibiotic impacts and risk thresholds on aquatic microbiomes and resistomes","authors":"Guomin Jin, Xingshuo Wang, Rongxin Cui, Shengyu Yuan, Meilun Wang, Zeyou Chen","doi":"10.1016/j.watres.2025.123262","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123262","url":null,"abstract":"Understanding the impacts of environmentally relevant low-level antibiotics on aquatic microbiomes and resistomes is crucial for risk assessment of anthropogenic antibiotic contamination. Here, we investigated the effects of seven subinhibitory concentrations of trimethoprim and lincomycin (10 ng/L to 10 mg/L), individually and in combination, on surface water microcosms, using unspiked samples as controls. Metagenomic sequencing revealed a decrease in bacterial community α-diversity and an increase in resistome <em>α</em>-diversity with rising antibiotic concentrations. Notably, the <em>β</em>-diversity of both bacterial communities and resistomes exhibited a biphasic response, decreasing and then increasing with breakpoint concentrations of 2.73 μg/L and 0.68 μg/L, respectively. We also observed concentration-dependent increases in certain metagenome-assembled antibiotic-resistant bacteria (MAARB) and antibiotic resistance genes (ARGs), with minimum selective concentrations (MSCs) of 2.28 μg/L for trimethoprim targeting <em>OXA-21</em> and 32.4 μg/L for lincomycin targeting <em>erm(F)</em>. Among various metrics for identifying risk thresholds that induce significant changes in microbial taxa, resistomes, individual ARGs, and MAARB, the breakpoint concentration derived from resistome β-diversity was the most conservative. We propose integrating this metric into environmental risk assessment frameworks for antibiotics. Our study provides a systematic evaluation of antibiotic impacts on aquatic microbiomes and resistomes, offering key insights for refining risk assessments of antibiotic contamination in aquatic environments.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"12 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-09DOI: 10.1016/j.watres.2025.123214
Zhibin Lu, Weichuan Lin, Qiang Li, Qingyang Wu, Zhiming Ren, Changkao Mu, Chunlin Wang, Ce Shi, Yangfang Ye
{"title":"Corrigendum to “Recirculating aquaculture system as microbial community and water quality management strategy: a rearing mode for improved viability of crab larvae” [Water Research Volume 252, Mar 2024]","authors":"Zhibin Lu, Weichuan Lin, Qiang Li, Qingyang Wu, Zhiming Ren, Changkao Mu, Chunlin Wang, Ce Shi, Yangfang Ye","doi":"10.1016/j.watres.2025.123214","DOIUrl":"10.1016/j.watres.2025.123214","url":null,"abstract":"","PeriodicalId":443,"journal":{"name":"Water Research","volume":"275 ","pages":"Article 123214"},"PeriodicalIF":11.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-09DOI: 10.1016/j.watres.2025.123259
Mohamed Ibrahim Azzam , Mohamed A. Nasr-Eldin , Fafy A. Mohammed , Kawthar A. Omran
In this study, a novel Malk1 phage, was isolated and characterized for its ability to target a broad range of multidrug-resistant (MDR) bacterial strains. Malk1, classified within the Siphoviridae family, showed lytic activity with a capsid diameter of 84 nm and a tail length of 205 nm. It demonstrated a short latent period of 18 min and a burst size of 102 virions per infected cell. The phage exhibited strong thermal stability up to 60 °C and maintained activity across a pH range of 6.0–10.0. However, exposure to hand soap and 70 % ethanol reduced its titers by over 94 % and 97 %, respectively. Malk1 lysed 92 % of the tested bacterial strains and had a genome of 44.3 kb, encoding 75 open reading frames (ORFs), with no genes for toxins, antibiotic resistance, or CRISPR elements, making it a virulent phage. A novel design utilizing immobilized polyvalent Malk1 phage on plastic sheets demonstrated superior efficacy in reducing multi-drug resistant (MDR) bacterial strains. The removal efficiencies for C.freundii (78–91 %), E.coli (74–85 %), S.enterica (60–76 %), and S.flexneri (63–72 %) were significantly higher compared to purified phage, which achieved removal efficiencies of 63–69 %, 58–66 %, 52–63 %, and 55–68 %, respectively, after 6 to 8 h. Furthermore, the immobilized phage treatment led to a 94.1 % improvement in the removal of physicochemical pollutants in wastewater, significantly surpassing the 65.3 % removal achieved with purified phage. The treatment process led to significant improvements in water quality, achieving an average removal efficiency of 71.1 % for electrical conductivity, 67.52 % for turbidity, 73.67 % for total dissolved solids (TDS), 88.02 % for biochemical oxygen demand (BOD), and 81.88 % for ammonia (NH₃). Additionally, the average dissolved oxygen (DO) levels increased by 79.17 % compared to untreated wastewater. These findings highlight the promising potential of Malk1 phage, particularly in its immobilized form, for pathogen control and enhancing water quality.
Originality-significance statement
We introduce the newly isolated polyvalent Malk1 phage, which has been thoroughly genome characterized and annotated. Immobilized Malk1 phage has proven effective in controlling drainage water pollution and addressing global concerns for irrigation water quality. Our experiments successfully reduced several multi-drug-resistant (MDR) bacterial strains in highly polluted drainage water, leading to significant improvements in water quality in a short time and at an affordable cost, facilitated by our innovative laboratory design.
{"title":"Whole genome sequencing of the novel polyvalent bacteriophage Malk1: A powerful biocontrol agent for water pollution","authors":"Mohamed Ibrahim Azzam , Mohamed A. Nasr-Eldin , Fafy A. Mohammed , Kawthar A. Omran","doi":"10.1016/j.watres.2025.123259","DOIUrl":"10.1016/j.watres.2025.123259","url":null,"abstract":"<div><div>In this study, a novel Malk1 phage, was isolated and characterized for its ability to target a broad range of multidrug-resistant (MDR) bacterial strains. Malk1, classified within the <em>Siphoviridae</em> family, showed lytic activity with a capsid diameter of 84 nm and a tail length of 205 nm. It demonstrated a short latent period of 18 min and a burst size of 102 virions per infected cell. The phage exhibited strong thermal stability up to 60 °C and maintained activity across a pH range of 6.0–10.0. However, exposure to hand soap and 70 % ethanol reduced its titers by over 94 % and 97 %, respectively. Malk1 lysed 92 % of the tested bacterial strains and had a genome of 44.3 kb, encoding 75 open reading frames (ORFs), with no genes for toxins, antibiotic resistance, or CRISPR elements, making it a virulent phage. A novel design utilizing immobilized polyvalent Malk1 phage on plastic sheets demonstrated superior efficacy in reducing multi-drug resistant (MDR) bacterial strains. The removal efficiencies for <em>C.freundii</em> (78–91 %), <em>E.coli</em> (74–85 %), <em>S.enterica</em> (60–76 %), and <em>S.flexneri</em> (63–72 %) were significantly higher compared to purified phage, which achieved removal efficiencies of 63–69 %, 58–66 %, 52–63 %, and 55–68 %, respectively, after 6 to 8 h. Furthermore, the immobilized phage treatment led to a 94.1 % improvement in the removal of physicochemical pollutants in wastewater, significantly surpassing the 65.3 % removal achieved with purified phage. The treatment process led to significant improvements in water quality, achieving an average removal efficiency of 71.1 % for electrical conductivity, 67.52 % for turbidity, 73.67 % for total dissolved solids (TDS), 88.02 % for biochemical oxygen demand (BOD), and 81.88 % for ammonia (NH₃). Additionally, the average dissolved oxygen (DO) levels increased by 79.17 % compared to untreated wastewater. These findings highlight the promising potential of Malk1 phage, particularly in its immobilized form, for pathogen control and enhancing water quality.</div></div><div><h3>Originality-significance statement</h3><div>We introduce the newly isolated polyvalent Malk1 phage, which has been thoroughly genome characterized and annotated. Immobilized Malk1 phage has proven effective in controlling drainage water pollution and addressing global concerns for irrigation water quality. Our experiments successfully reduced several multi-drug-resistant (MDR) bacterial strains in highly polluted drainage water, leading to significant improvements in water quality in a short time and at an affordable cost, facilitated by our innovative laboratory design.</div></div>","PeriodicalId":443,"journal":{"name":"Water Research","volume":"276 ","pages":"Article 123259"},"PeriodicalIF":11.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143375273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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