Pub Date : 2025-03-18DOI: 10.1016/j.watres.2025.123513
Eduardo De Gerónimo, Agustín Mosca, Franco M. Cabrerizo, Ronald Vargas
Chlorination is a common method for drinking water disinfection due to its efficiency and low cost. The strong oxidative properties of chlorine can lead to reactions with dissolved organic compounds, resulting in various transformation products. This study investigates the chlorination-induced degradation of the sulfonylurea herbicides metsulfuron-methyl and chlorimuron-ethyl, which are frequently found in surface and groundwater. The degradation of these herbicides follows a second-order kinetic model. The apparent second-order rate constants for metsulfuron-methyl range from 3.2 to 244 M⁻¹ s⁻¹, while those for chlorimuron-ethyl range from 2.2 to 287.7 M⁻¹ s⁻¹ within a pH range of 4 to 9. Reaction with HClO effectively reduced the concentration of pesticides. Under acidic conditions, the reaction was significantly enhanced, likely due to hydrolysis or changes in the speciation of the organic compounds. In fact, the rate constant under acidic conditions was approximately 35 and 27 times higher than the reaction rate at more neutral pH for chlorimuron-ethyl and metsulfuron-methyl, respectively. The reaction rate with ClO⁻ approached zero for both herbicides, suggesting a minor or negligible pathway involving the hypochlorite anion. Mass spectrometry identified six chlorination products for metsulfuron-methyl and five for chlorimuron-ethyl. Although the specific reaction mechanisms were not fully elucidated, these products provided valuable insights into the fate of sulfonylureas under chlorination. Under typical disinfection conditions (pH 7 and 4 mg L⁻¹ chlorine), the half-lives of 17.8 minutes for metsulfuron-methyl and 26.6 minutes for chlorimuron-ethyl demonstrate the potential for effective degradation in relatively short timeframes. This study underscores the potential for effective removal of these herbicides in drinking water treatment and highlights the importance of evaluating degradation products over time, as they remain detectable even after seven days.
{"title":"Insights into Chlorination-Induced Degradation of Sulfonylurea Herbicides: Unraveling Kinetics and Intermediates during Water Treatment","authors":"Eduardo De Gerónimo, Agustín Mosca, Franco M. Cabrerizo, Ronald Vargas","doi":"10.1016/j.watres.2025.123513","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123513","url":null,"abstract":"Chlorination is a common method for drinking water disinfection due to its efficiency and low cost. The strong oxidative properties of chlorine can lead to reactions with dissolved organic compounds, resulting in various transformation products. This study investigates the chlorination-induced degradation of the sulfonylurea herbicides metsulfuron-methyl and chlorimuron-ethyl, which are frequently found in surface and groundwater. The degradation of these herbicides follows a second-order kinetic model. The apparent second-order rate constants for metsulfuron-methyl range from 3.2 to 244 M⁻¹ s⁻¹, while those for chlorimuron-ethyl range from 2.2 to 287.7 M⁻¹ s⁻¹ within a pH range of 4 to 9. Reaction with HClO effectively reduced the concentration of pesticides. Under acidic conditions, the reaction was significantly enhanced, likely due to hydrolysis or changes in the speciation of the organic compounds. In fact, the rate constant under acidic conditions was approximately 35 and 27 times higher than the reaction rate at more neutral pH for chlorimuron-ethyl and metsulfuron-methyl, respectively. The reaction rate with ClO⁻ approached zero for both herbicides, suggesting a minor or negligible pathway involving the hypochlorite anion. Mass spectrometry identified six chlorination products for metsulfuron-methyl and five for chlorimuron-ethyl. Although the specific reaction mechanisms were not fully elucidated, these products provided valuable insights into the fate of sulfonylureas under chlorination. Under typical disinfection conditions (pH 7 and 4 mg L⁻¹ chlorine), the half-lives of 17.8 minutes for metsulfuron-methyl and 26.6 minutes for chlorimuron-ethyl demonstrate the potential for effective degradation in relatively short timeframes. This study underscores the potential for effective removal of these herbicides in drinking water treatment and highlights the importance of evaluating degradation products over time, as they remain detectable even after seven days.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"34 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143654160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial oxidation of short-chain gaseous alkanes (SCGAs, including ethane, propane and butane) are important sinks to mitigate the emission of SCGAs to the atmosphere. ‘Candidatus Alkanivorans nitratireducens’ has been discovered to be capable of utilizing nitrate as an electron acceptor to anaerobically oxidize these SCGAs. However, little is known about its metabolic diversity in sulfate reduction, despite sulfate being widely present in both marine and freshwater ecosystems. Here, we show that sulfate can be reduced by ‘Ca. A. nitratireducens’ and as an alternative electron acceptor. Genomic analysis confirmed that the genome of ‘Ca. A. nitratireducens’ harbour genes involved in sulfate reduction. Short-term incubation of the enriched ‘Ca. A. nitratireducens’ showed immediate consumption of propane and sulfate, suggesting the capability of ‘Ca. A. nitratireducens’ to utilize sulfate as an electron acceptor. Long-term incubation further confirmed its ability to utilize sulfate. However, propane oxidation rates and sulfate reduction rates gradually decreased during the long-term incubation, accompanied by the decrease of relative abundance of ‘Ca. A. nitratireducens’. After the long-term adaptation with sulfate as the sole acceptor, both anaerobic propane oxidation and nitrate reduction capability of ‘Ca. A. nitratireducens’ can be partly recovered by switching the electron acceptor back from sulfate to nitrate. Overall, this study indicates sulfate can be utilized but is not the preferred electron acceptor for ‘Ca. A. nitratireducens’. The findings deepen our understanding on the metabolic flexibility of ‘Ca. A. nitratireducens’.
{"title":"Sulfate-reducing capability of nitrate-dependent anaerobic gaseous alkanes degrader","authors":"Xiawei Liu, Zhiguo Yuan, Mengxiong Wu, Jianhua Guo","doi":"10.1016/j.watres.2025.123507","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123507","url":null,"abstract":"Microbial oxidation of short-chain gaseous alkanes (SCGAs, including ethane, propane and butane) are important sinks to mitigate the emission of SCGAs to the atmosphere. ‘<em>Candidatus</em> Alkanivorans nitratireducens’ has been discovered to be capable of utilizing nitrate as an electron acceptor to anaerobically oxidize these SCGAs. However, little is known about its metabolic diversity in sulfate reduction, despite sulfate being widely present in both marine and freshwater ecosystems. Here, we show that sulfate can be reduced by ‘<em>Ca.</em> A. nitratireducens’ and as an alternative electron acceptor. Genomic analysis confirmed that the genome of ‘<em>Ca.</em> A. nitratireducens’ harbour genes involved in sulfate reduction. Short-term incubation of the enriched ‘<em>Ca.</em> A. nitratireducens’ showed immediate consumption of propane and sulfate, suggesting the capability of ‘<em>Ca.</em> A. nitratireducens’ to utilize sulfate as an electron acceptor. Long-term incubation further confirmed its ability to utilize sulfate. However, propane oxidation rates and sulfate reduction rates gradually decreased during the long-term incubation, accompanied by the decrease of relative abundance of ‘<em>Ca.</em> A. nitratireducens’. After the long-term adaptation with sulfate as the sole acceptor, both anaerobic propane oxidation and nitrate reduction capability of ‘<em>Ca.</em> A. nitratireducens’ can be partly recovered by switching the electron acceptor back from sulfate to nitrate. Overall, this study indicates sulfate can be utilized but is not the preferred electron acceptor for ‘<em>Ca.</em> A. nitratireducens’. The findings deepen our understanding on the metabolic flexibility of ‘<em>Ca.</em> A. nitratireducens’.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"12 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143654191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-18DOI: 10.1016/j.watres.2025.123512
R. Thomson, K. Close, A. Riley, D.J. Batstone, A. Oehmen
Existing enhanced biological phosphorus removal (EBPR) models do not fully describe the metabolism of fermentative polyphosphate accumulating organisms (fPAOs), particularly under mixed substrate conditions representative of fermentation-enhanced EBPR (F-EBPR) processes. This study presents a steady-state metabolic model integrating anaerobic amino acid (AA) fermentation and storage processes in fPAOs. The model identifies key metabolic interactions underlying fPAO metabolism, prioritising substrate accumulation over fermentation. This results in significant changes to ATP and reduction-oxidation (redox) flows as compared to when relying on previous AA fermentation models typically used to describe fPAO metabolism, with medium-chain-length (MCL) polyhydroxyalkanoate (PHA) formation and polyphosphate (polyP) consumption acting as important electron and energy management mechanisms, respectively. Succinate, rather than volatile fatty acids (VFAs), was identified as the more likely synergetic substrate between fermentative and conventional PAOs (cPAOs) under these conditions. Moreover, conditions favourable of VFA efflux by fPAOs may also favour a shift away from a polyP accumulation to a fermentation dominant metabolism. Further work is required to verify the role of MCL-PHA fractions, alongside the contribution of free intracellular AA accumulation as compared to polymers such as cyanophycin or polyglutamate on fPAO metabolism. This metabolic model provides a framework for better understanding the role of fPAOs and their interactions with cPAOs within EBPR processes, informing future modelling and optimisation of F-EBPR systems.
{"title":"Metabolic Modelling of Anaerobic Amino Acid Uptake and Storage by Fermentative Polyphosphate Accumulating Organisms","authors":"R. Thomson, K. Close, A. Riley, D.J. Batstone, A. Oehmen","doi":"10.1016/j.watres.2025.123512","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123512","url":null,"abstract":"Existing enhanced biological phosphorus removal (EBPR) models do not fully describe the metabolism of fermentative polyphosphate accumulating organisms (fPAOs), particularly under mixed substrate conditions representative of fermentation-enhanced EBPR (F-EBPR) processes. This study presents a steady-state metabolic model integrating anaerobic amino acid (AA) fermentation and storage processes in fPAOs. The model identifies key metabolic interactions underlying fPAO metabolism, prioritising substrate accumulation over fermentation. This results in significant changes to ATP and reduction-oxidation (redox) flows as compared to when relying on previous AA fermentation models typically used to describe fPAO metabolism, with medium-chain-length (MCL) polyhydroxyalkanoate (PHA) formation and polyphosphate (polyP) consumption acting as important electron and energy management mechanisms, respectively. Succinate, rather than volatile fatty acids (VFAs), was identified as the more likely synergetic substrate between fermentative and conventional PAOs (cPAOs) under these conditions. Moreover, conditions favourable of VFA efflux by fPAOs may also favour a shift away from a polyP accumulation to a fermentation dominant metabolism. Further work is required to verify the role of MCL-PHA fractions, alongside the contribution of free intracellular AA accumulation as compared to polymers such as cyanophycin or polyglutamate on fPAO metabolism. This metabolic model provides a framework for better understanding the role of fPAOs and their interactions with cPAOs within EBPR processes, informing future modelling and optimisation of F-EBPR systems.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"189 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-18DOI: 10.1016/j.watres.2025.123511
Christopher Keneally, Daniel Chilton, Tyler N. Dornan, Stephen P. Kidd, Virginie Gaget, Adam Toomes, Charlotte Lassaline, Reuben Petrovski, Lisa Wood, Justin D. Brookes
Microorganisms drive essential biogeochemical processes in aquatic ecosystems and are sensitive to both salinity and hydrological changes. As climate change and anthropogenic activities alter hydrology and salinity worldwide, understanding microbial ecology and metabolism becomes increasingly important for managing aquatic ecosystems. Biogeochemical processes were investigated on sediment microbial communities during a significant flood event in the hypersaline Coorong lagoon, South Australia (the largest in the Murray-Darling Basin since 1956). Samples from six sites across a salinity gradient were collected before and during flooding in 2022. To assess changes in microbial taxonomy and metabolic function, 16S rRNA amplicon sequencing was employed alongside untargeted liquid chromatography–mass spectrometry (LC-MS) to assess changes in microbial taxonomy and metabolic function. Results showed a decrease in microbial richness and diversity during flooding, especially in hypersaline conditions. Pre-flood communities were enriched with osmolyte-degrading and methanogenic taxa, alongside osmoprotectant metabolites, such as glycine betaine and choline. Flood conditions favored taxa such as Halanaerobiaceae and Beggiatoaceae, inducing inferred metagenomic shifts indicative of sulfate and nitrogen reduction pathways, while also enriching a greater diversity of metabolites including Gly-Phe dipeptides and guanine. This study demonstrates that integrating metabolomics with microbial community analysis enhances understanding of ecosystem responses to disturbance. These findings suggest microbial communities rapidly change in response to salinity reductions while maintaining key biogeochemical functions. Such insights are valuable for ecosystem management and predictive modelling under environmental stressors such as flooding.
{"title":"Multi-Omics Reveal Microbial Succession and Metabolomic Adaptations to Flood in a Hypersaline Coastal Lagoon","authors":"Christopher Keneally, Daniel Chilton, Tyler N. Dornan, Stephen P. Kidd, Virginie Gaget, Adam Toomes, Charlotte Lassaline, Reuben Petrovski, Lisa Wood, Justin D. Brookes","doi":"10.1016/j.watres.2025.123511","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123511","url":null,"abstract":"Microorganisms drive essential biogeochemical processes in aquatic ecosystems and are sensitive to both salinity and hydrological changes. As climate change and anthropogenic activities alter hydrology and salinity worldwide, understanding microbial ecology and metabolism becomes increasingly important for managing aquatic ecosystems. Biogeochemical processes were investigated on sediment microbial communities during a significant flood event in the hypersaline Coorong lagoon, South Australia (the largest in the Murray-Darling Basin since 1956). Samples from six sites across a salinity gradient were collected before and during flooding in 2022. To assess changes in microbial taxonomy and metabolic function, 16S rRNA amplicon sequencing was employed alongside untargeted liquid chromatography–mass spectrometry (LC-MS) to assess changes in microbial taxonomy and metabolic function. Results showed a decrease in microbial richness and diversity during flooding, especially in hypersaline conditions. Pre-flood communities were enriched with osmolyte-degrading and methanogenic taxa, alongside osmoprotectant metabolites, such as glycine betaine and choline. Flood conditions favored taxa such as <em>Halanaerobiaceae</em> and <em>Beggiatoaceae</em>, inducing inferred metagenomic shifts indicative of sulfate and nitrogen reduction pathways, while also enriching a greater diversity of metabolites including Gly-Phe dipeptides and guanine. This study demonstrates that integrating metabolomics with microbial community analysis enhances understanding of ecosystem responses to disturbance. These findings suggest microbial communities rapidly change in response to salinity reductions while maintaining key biogeochemical functions. Such insights are valuable for ecosystem management and predictive modelling under environmental stressors such as flooding.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"197 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-18DOI: 10.1016/j.watres.2025.123514
Yoshiyasu Takefuji
This paper critically examines the analysis conducted by Maußner et al. on AI analysis, particularly their interpretation of feature importances derived from various machine learning models using SHAP (SHapley Additive exPlanations). Although SHAP aids in interpretability, it is subject to model-specific biases that can misrepresent relationships between variables. The paper emphasizes the lack of ground truth values in feature importance assessments and calls for careful consideration of statistical methodologies, including robust nonparametric approaches. By advocating for the use of Spearman's correlation with p-values and Kendall's tau with p-values, this work aims to strengthen the integrity of findings in machine learning studies, ensuring that conclusions drawn are reliable and actionable.
{"title":"Reevaluating Feature Importance in Machine Learning: Concerns Regarding SHAP Interpretations in the Context of the EU Artificial Intelligence Act","authors":"Yoshiyasu Takefuji","doi":"10.1016/j.watres.2025.123514","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123514","url":null,"abstract":"This paper critically examines the analysis conducted by Maußner et al. on AI analysis, particularly their interpretation of feature importances derived from various machine learning models using SHAP (SHapley Additive exPlanations). Although SHAP aids in interpretability, it is subject to model-specific biases that can misrepresent relationships between variables. The paper emphasizes the lack of ground truth values in feature importance assessments and calls for careful consideration of statistical methodologies, including robust nonparametric approaches. By advocating for the use of Spearman's correlation with p-values and Kendall's tau with p-values, this work aims to strengthen the integrity of findings in machine learning studies, ensuring that conclusions drawn are reliable and actionable.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"24 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143640263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-16DOI: 10.1016/j.watres.2025.123510
Ziyi Zhao, Yanan Mi, Shili Wang, Xuedong Du, Qingrui Zhang
We firstly present a novel strategy for enhancing fluoride removal from contaminated water using defect-engineered UiO-66 (Zr-MOFs), emphasizing the crucial role of pKa in modulator selection. By utilizing modulators with varying pKa values-specifically benzimidazole (BI), benzoic acid (BA), and acetic acid (AA)-we synthesized defect-rich Act-UiO-66-M(X). The higher pKa of BI facilitated greater defect formation, resulting in significantly improved adsorption capacity and faster diffusion rates. Act-UiO-66-BI(8), modulated with BI, showed a higher intensity peak at g = 2.003 in ESR analysis, indicating more oxygen vacancies. Its fluoride adsorption capacity reached 93.59 mg F/g, nearly six times higher than commercial ZrO2, with rapid kinetics—evidenced by a kinetic rate constant (kint) of 2.64 mg/g·min0.5 and equilibrium achieved within 10 minutes. The kinetic performance was enhanced by 270% compared to raw Act-UiO-66. Furthermore, Act-UiO-66-BI(8) demonstrated high selectivity and stability in high-salinity environments, with a Kd coefficient consistently exceeding 17,900 mL/g. The study highlights that selecting modulators based on pKa enhances defect formation, improving active site exposure and pore diffusion, as confirmed by DFT calculations and XPS analysis. The ability of Act-UiO-66-BI(8) to treat up to 1160 kg of wastewater per kg of adsorbent highlights its potential for large-scale water purification, showcasing a promising approach for developing high-performance MOF materials.
{"title":"The High pKa-Guided Defect Engineering: Improving Fluoride Removal in Actual Scenarios by Benzimidazole Modulated Metal-Organic Frameworks","authors":"Ziyi Zhao, Yanan Mi, Shili Wang, Xuedong Du, Qingrui Zhang","doi":"10.1016/j.watres.2025.123510","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123510","url":null,"abstract":"We firstly present a novel strategy for enhancing fluoride removal from contaminated water using defect-engineered UiO-66 (Zr-MOFs), emphasizing the crucial role of pKa in modulator selection. By utilizing modulators with varying pKa values-specifically benzimidazole (BI), benzoic acid (BA), and acetic acid (AA)-we synthesized defect-rich Act-UiO-66-M(X). The higher pKa of BI facilitated greater defect formation, resulting in significantly improved adsorption capacity and faster diffusion rates. Act-UiO-66-BI(8), modulated with BI, showed a higher intensity peak at g = 2.003 in ESR analysis, indicating more oxygen vacancies. Its fluoride adsorption capacity reached 93.59 mg F/g, nearly six times higher than commercial ZrO<sub>2</sub>, with rapid kinetics—evidenced by a kinetic rate constant (k<sub>int</sub>) of 2.64 mg/g·min<sup>0.5</sup> and equilibrium achieved within 10 minutes. The kinetic performance was enhanced by 270% compared to raw Act-UiO-66. Furthermore, Act-UiO-66-BI(8) demonstrated high selectivity and stability in high-salinity environments, with a K<sub>d</sub> coefficient consistently exceeding 17,900 mL/g. The study highlights that selecting modulators based on pKa enhances defect formation, improving active site exposure and pore diffusion, as confirmed by DFT calculations and XPS analysis. The ability of Act-UiO-66-BI(8) to treat up to 1160 kg of wastewater per kg of adsorbent highlights its potential for large-scale water purification, showcasing a promising approach for developing high-performance MOF materials.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"69 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143635596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-15DOI: 10.1016/j.watres.2025.123509
Guangyu Li, Varun Srinivasan, Nicholas B. Tooker, Dongqi Wang, Annalisa Onnis-Hayden, Charles Bott, Paul Dombrowski, Ameet Pinto, April Z. Gu
Side-Stream Enhanced Biological Phosphorus Removal (S2EBPR) has emerged as a promising technology addressing certain challenges of conventional Enhanced Biological Phosphorus Removal (EBPR), notably stability in phosphorus removal, yet the underlying mechanisms are not fully understood. Metagenomic analysis presents a powerful approach to elucidate community-level metabolic differences between EBPR and S2EBPR configurations. In this study, we compared three EBPR and three S2EBPR activated sludge communities using metagenomic analysis at taxonomy, key functional pathways/genes, and polyphosphate-metabolism marker genes. Our analysis revealed larger genus-level diversity variance in S2EBPR communities, indicating distinct microbial community compositions influenced by different operational configurations. A higher diversity index in the S2EBPR than the EBPR was observed, and a higher Ca. Accumulibacter abundance was detected in EBPRs, whereas the fermentative candidate PAOs genera, including Ca. Phosphoribacter and Ca. Promineifilum, were more abundant in S2EBPR systems. EBPR and S2EBPR groups displayed similar gene and pathway abundance patterns related to core metabolisms essential for carbon and nitrogen metabolism. PolyP-metabolism marker gene phylogeny analysis suggested that exopolyphosphatase gene (ppx) showed better distinctions between EBPR and S2EBPR communities than polyphosphate kinase gene (ppk). This also highlighted the needs in fine-cale microdiversity analysis and finding novel Ca. Accumulibacter clades and species as resolved using the ppk gene. These findings provide valuable insights into AS community dynamics and metabolic functionalities, paving the way for further research into optimizing phosphorus removal processes in wastewater treatment systems.
{"title":"Metagenomic Analysis Revealed Community-level Metabolic Differences Between Full-scale EBPR and S2EBPR Systems","authors":"Guangyu Li, Varun Srinivasan, Nicholas B. Tooker, Dongqi Wang, Annalisa Onnis-Hayden, Charles Bott, Paul Dombrowski, Ameet Pinto, April Z. Gu","doi":"10.1016/j.watres.2025.123509","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123509","url":null,"abstract":"Side-Stream Enhanced Biological Phosphorus Removal (S2EBPR) has emerged as a promising technology addressing certain challenges of conventional Enhanced Biological Phosphorus Removal (EBPR), notably stability in phosphorus removal, yet the underlying mechanisms are not fully understood. Metagenomic analysis presents a powerful approach to elucidate community-level metabolic differences between EBPR and S2EBPR configurations. In this study, we compared three EBPR and three S2EBPR activated sludge communities using metagenomic analysis at taxonomy, key functional pathways/genes, and polyphosphate-metabolism marker genes. Our analysis revealed larger genus-level diversity variance in S2EBPR communities, indicating distinct microbial community compositions influenced by different operational configurations. A higher diversity index in the S2EBPR than the EBPR was observed, and a higher <em>Ca.</em> Accumulibacter abundance was detected in EBPRs, whereas the fermentative candidate PAOs genera, including <em>Ca.</em> Phosphoribacter and <em>Ca.</em> Promineifilum, were more abundant in S2EBPR systems. EBPR and S2EBPR groups displayed similar gene and pathway abundance patterns related to core metabolisms essential for carbon and nitrogen metabolism. PolyP-metabolism marker gene phylogeny analysis suggested that exopolyphosphatase gene (<em>ppx</em>) showed better distinctions between EBPR and S2EBPR communities than polyphosphate kinase gene (<em>ppk</em>). This also highlighted the needs in fine-cale microdiversity analysis and finding novel <em>Ca.</em> Accumulibacter clades and species as resolved using the <em>ppk</em> gene. These findings provide valuable insights into AS community dynamics and metabolic functionalities, paving the way for further research into optimizing phosphorus removal processes in wastewater treatment systems.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"183 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ferrate (Fe(VI)) can provide oxidation and in-situ coagulation/adsorption for the removal of emerging contaminants and natural organic matter, and can be used in conjunction with ultrafiltration (UF) membrane to enhance the removal of composite contaminants and mitigate UF membrane fouling. Based on the Fe(VI) multifunctional integration characteristics, the study objectively and comprehensively verified the gradient purification of surface water and the UF membrane fouling mitigation by Fe(VI)-UF and sulfite (S(IV)) activated Fe(VI) (S(IV)/Fe(VI))-UF, elucidated the effect of sedimentation unit on the UF mechanism and the membrane fouling behaviors, and revealed the free energy changes throughout the UF process. The experimental results demonstrated that S(IV)/Fe(VI)-UF showed superior purification performance and UF membrane fouling mitigation than Fe(VI)-UF. S(IV)/Fe(VI)-UF achieved removals of sulfamethoxazole, DOC, and UV254 up to 77.73%, 61.86%, and 86.33%, and was able to significantly mitigate UF membrane fouling by prolonging the transition stage and positively shifting the interfacial free energy. Innovatively, the absence of a sedimentation unit was found to adversely affect the initial stages of Fe(VI)-UF and S(IV)/Fe(VI)-UF by lowering the energy barriers, while negligibly affecting cake filtration. Additionally, the water treatment cost of S(IV)/Fe(VI) was 0.3 yuan per ton, indicating notable economic benefits and engineering potential. While deepening the understanding of Fe(VI) multifunctional integration characteristics, the above results provided theoretical and data support for S(IV)/Fe(VI)-UF and Fe(VI)-UF treatment of surface water, and enriched the application scenarios of Fe(VI)-related technologies.
{"title":"Gradient purification of surface water and ultrafiltration membrane fouling mitigation based on Fe(VI) multifunctional integration characteristics: Is a sedimentation unit necessary?","authors":"Kunyu Chen, Jie Wang, Xiaojiang Huang, Ruimin Mu, Congwei Luo, Daoji Wu, Jing Liu, Jinsuo Lu, Xiaoxiang Cheng","doi":"10.1016/j.watres.2025.123508","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123508","url":null,"abstract":"Ferrate (Fe(VI)) can provide oxidation and in-situ coagulation/adsorption for the removal of emerging contaminants and natural organic matter, and can be used in conjunction with ultrafiltration (UF) membrane to enhance the removal of composite contaminants and mitigate UF membrane fouling. Based on the Fe(VI) multifunctional integration characteristics, the study objectively and comprehensively verified the gradient purification of surface water and the UF membrane fouling mitigation by Fe(VI)-UF and sulfite (S(IV)) activated Fe(VI) (S(IV)/Fe(VI))-UF, elucidated the effect of sedimentation unit on the UF mechanism and the membrane fouling behaviors, and revealed the free energy changes throughout the UF process. The experimental results demonstrated that S(IV)/Fe(VI)-UF showed superior purification performance and UF membrane fouling mitigation than Fe(VI)-UF. S(IV)/Fe(VI)-UF achieved removals of sulfamethoxazole, DOC, and UV<sub>254</sub> up to 77.73%, 61.86%, and 86.33%, and was able to significantly mitigate UF membrane fouling by prolonging the transition stage and positively shifting the interfacial free energy. Innovatively, the absence of a sedimentation unit was found to adversely affect the initial stages of Fe(VI)-UF and S(IV)/Fe(VI)-UF by lowering the energy barriers, while negligibly affecting cake filtration. Additionally, the water treatment cost of S(IV)/Fe(VI) was 0.3 yuan per ton, indicating notable economic benefits and engineering potential. While deepening the understanding of Fe(VI) multifunctional integration characteristics, the above results provided theoretical and data support for S(IV)/Fe(VI)-UF and Fe(VI)-UF treatment of surface water, and enriched the application scenarios of Fe(VI)-related technologies.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"14 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-03-15DOI: 10.1016/j.watres.2025.123506
Wenjie Wan, Hans-Peter Grossart, Qinglong L Wu, Xiang Xiong, Wenke Yuan, Weihong Zhang, Quanfa Zhang, Wenzhi Liu, Yuyi Yang
Global “Sustainable Development Goals” propose ambitious targets to protect water resource and provide clean water, whereas comprehensive understanding of restoration performance and ecological mechanisms are lacking for dredging adopted for purifying polluted waterbodies and maintaining navigation channels. Here, we conducted a global meta-analysis to estimate ecological restoration consequence of dredging as pollution mitigation and navigation channel maintenance measures using a dataset compiled from 191 articles covering 696 studies and 84 environmental and ecological parameters (e.g., pollutants and hydrobiontes). We confirm that dredging shows negative influences on 77.50% pollutants in the BA model (before dredging vs. after dredging) and 84.21% pollutants in the CI model (control vs. impact) as well as on sediment nutrient fluxes. Additionally, 57.14% attributes (i.e., richness, diversity, biomass, and density) of hydrobiontes in the BA model and 89.47% attributes of hydrobiontes in the CI model responded negatively to dredging. As a result, 76.32% of the pollutants and 61.11% of the hydrobiont attributes responded uniformly to dredging in the BA and CI models. Our findings emphasize that dredging generally decreases pollutants and mitigates algal blooms, controlling phosphorus is easier than controlling nitrogen by dredging, and attributes (i.e., richness, diversity, and biomass) of hydrobiontes (i.e., zooplankton, phytoplankton, and zoobenthos) are density-dependent in dredging-disturbed environments. Our findings broaden our knowledge on ecological restoration performance of dredging as a mitigation measure in global aquatic ecosystems, and these findings might be helpful to use and optimize dredging to efficiently and sustainably purify polluted aquatic ecosystems.
{"title":"Global meta-analysis deciphering ecological restoration performance of dredging: divergent variabilities of pollutants and hydrobiontes","authors":"Wenjie Wan, Hans-Peter Grossart, Qinglong L Wu, Xiang Xiong, Wenke Yuan, Weihong Zhang, Quanfa Zhang, Wenzhi Liu, Yuyi Yang","doi":"10.1016/j.watres.2025.123506","DOIUrl":"https://doi.org/10.1016/j.watres.2025.123506","url":null,"abstract":"Global “Sustainable Development Goals” propose ambitious targets to protect water resource and provide clean water, whereas comprehensive understanding of restoration performance and ecological mechanisms are lacking for dredging adopted for purifying polluted waterbodies and maintaining navigation channels. Here, we conducted a global meta-analysis to estimate ecological restoration consequence of dredging as pollution mitigation and navigation channel maintenance measures using a dataset compiled from 191 articles covering 696 studies and 84 environmental and ecological parameters (e.g., pollutants and hydrobiontes). We confirm that dredging shows negative influences on 77.50% pollutants in the BA model (before dredging vs. after dredging) and 84.21% pollutants in the CI model (control vs. impact) as well as on sediment nutrient fluxes. Additionally, 57.14% attributes (i.e., richness, diversity, biomass, and density) of <span><span>hydrobiontes</span><svg aria-label=\"Opens in new window\" focusable=\"false\" height=\"20\" viewbox=\"0 0 8 8\"><path d=\"M1.12949 2.1072V1H7V6.85795H5.89111V2.90281L0.784057 8L0 7.21635L5.11902 2.1072H1.12949Z\"></path></svg></span> in the BA model and 89.47% attributes of <span><span>hydrobiontes</span><svg aria-label=\"Opens in new window\" focusable=\"false\" height=\"20\" viewbox=\"0 0 8 8\"><path d=\"M1.12949 2.1072V1H7V6.85795H5.89111V2.90281L0.784057 8L0 7.21635L5.11902 2.1072H1.12949Z\"></path></svg></span> in the CI model responded negatively to dredging. As a result, 76.32% of the pollutants and 61.11% of the hydrobiont attributes responded uniformly to dredging in the BA and CI models. Our findings emphasize that dredging generally decreases pollutants and mitigates algal blooms, controlling phosphorus is easier than controlling nitrogen by dredging, and attributes (i.e., richness, diversity, and biomass) of hydrobiontes (i.e., zooplankton, phytoplankton, and zoobenthos) are density-dependent in dredging-disturbed environments. Our findings broaden our knowledge on ecological restoration performance of dredging as a mitigation measure in global aquatic ecosystems, and these findings might be helpful to use and optimize dredging to efficiently and sustainably purify polluted aquatic ecosystems.","PeriodicalId":443,"journal":{"name":"Water Research","volume":"23 1","pages":""},"PeriodicalIF":12.8,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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