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Molecular and phenotypic markers for pyramiding multiple traits in rice 水稻多性状金字塔化的分子和表型标记
IF 2.3 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1515/opag-2022-0187
N. Carsono, Reisyi R. Tambunan, S. Sari, N. Wicaksana
Abstract Gene pyramiding has been developed for effective resistant genes, such as bacterial blight resistance genes. However, in this report, pyramiding for multiple traits is presented. We evaluated 28 pyramided rice lines derived from 7 hybridizations of valuable parents for high yield potential, early maturity, aroma, high grain quality, and resistance to brown planthopper using both simple-sequence repeat markers and phenotypic markers. Pyramided rice genotypes were evaluated with RM282 molecular marker linked to GW3 gene associated with grain weight, RM259 (number of panicles), RM3600 (length of panicles), RM3701 (short panicles), RM19414 (Hd3), RM7601 (Hd2), RM190, and SSIIa (waxy), IFAP and ESP (aroma), RM586 (Bph3 and Bph4), and RM8213 (Qpbh4 and Bph17(t)). Eight genotypes (i.e., #9, #15, #16, #22, #25, #26, #27, and #28) were confirmed to have desired genes associated with multiple important traits based on molecular markers and morpho-agronomic traits. A strong correlation was found between RM19414 and heading date, between specific markers for aroma and sensory test. Meanwhile, a weak correlation was found between RM259 with panicle number, RM586, and RM8213 with trichome density. As presented in this study, gene pyramiding promises a new hope to combine multiple traits in rice breeding. Promising rice lines will be incorporated in the multiple traits breeding program.
摘要针对有效的抗性基因,如白叶枯病抗性基因,已经开发了基因金字塔。然而,在本报告中,提出了多个性状的金字塔。我们使用简单序列重复标记和表型标记评估了来自有价值亲本的7个杂交的28个吡喃型水稻品系的高产潜力、早熟性、香气、高谷质和对褐飞虱的抗性。用与粒重相关的GW3基因连接的RM282分子标记、RM259(穗数)、RM3600(穗长)、RM3701(短穗)、RM19414(Hd3)、RM7601(Hd2)、RM190和SSIIa(蜡质)、IFAP和ESP(香气)、RM586(Bph3和Bph4)和RM8213(Qpbh4和Bph17(t))评估了金字塔型水稻的基因型。基于分子标记和形态农艺性状,8种基因型(即#9、#15、#16、#22、#25、#26、#27和#28)被证实具有与多个重要性状相关的所需基因。RM19414与抽穗期、香气和感官测试的特异性标记之间存在很强的相关性。同时,RM259与穗数、RM586和RM8213与毛密度的相关性较弱。正如这项研究所提出的,基因聚合有望为水稻育种中结合多种性状带来新的希望。有希望的水稻品系将被纳入多重性状育种计划。
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引用次数: 0
Consumption profile of organic fruits and vegetables by a Portuguese consumer’s sample 一个葡萄牙消费者样本的有机水果和蔬菜的消费概况
Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1515/opag-2022-0217
André Oliveira, Ana Mendes, Jorge Lameiras, Pedro Mendes-Moreira, Goreti Botelho
Abstract The consumption of fruits and vegetables has been considered very important for human health. This research aimed to study consumption habits of fruits and vegetables from organic farming (OFV) within a convenience sample of Portuguese adults, including reasons for consumption, most valued mode of production sources, frequency of use, knowledge about characteristics and benefits, and information sources. An online questionnaire containing 30 questions was originally developed and shared on social networks and 300 questionnaires were obtained. The organic vegetables identified as the most consumed were lettuce (93.5%), potato (92%), and tomato (92%); the most consumed organic fruits were orange (83%), lemon (82%), and strawberry (82%). The strongest motivations to consume OFV include environmental benefits (57%) and health benefits (94%), namely the prevention of high total cholesterol (71%), the prevention of cardiovascular diseases (69%), and obesity prevention (68%). Regarding the level of information about the nutritional and chemical properties of OFV, 86% of the respondents consider themselves informed people. Meanwhile, there still are 33% of the respondents revealing no concern about the farming practices. As so, there is an opportunity to increase literacy about these products, to raise awareness about the benefits of organic products, and to promote higher consumption of OFV products, supported in the arguments of perceived positive impact of organic agriculture on ecosystems and human health.
水果和蔬菜的食用一直被认为对人体健康非常重要。本研究旨在研究葡萄牙成人有机农业(OFV)水果和蔬菜的消费习惯,包括消费原因,最受重视的生产方式来源,使用频率,对特征和益处的了解以及信息来源。最初开发了一份包含30个问题的在线问卷,并在社交网络上分享,获得了300份问卷。被认为消费最多的有机蔬菜是生菜(93.5%)、土豆(92%)和番茄(92%);食用最多的有机水果是橙子(83%)、柠檬(82%)和草莓(82%)。食用OFV的最强烈动机包括环境效益(57%)和健康效益(94%),即预防高总胆固醇(71%)、预防心血管疾病(69%)和预防肥胖(68%)。关于OFV的营养和化学特性的信息水平,86%的受访者认为自己是知情人士。与此同时,仍有33%的受访者表示不担心耕作方式。因此,有机会提高对这些产品的认识,提高对有机产品益处的认识,并促进OFV产品的更高消费,这些都得到了有机农业对生态系统和人类健康产生积极影响的论点的支持。
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引用次数: 1
Analysing the sustainability of swamp buffalo (Bubalus bubalis carabauesis) farming as a protein source and germplasm 分析沼泽水牛(Bubalus bubalis carabauesis)养殖作为蛋白质来源和种质的可持续性
Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1515/opag-2022-0224
Eni Siti Rohaeni, Arif Dwi Santoso, Ening Ariningsih, Neni Widaningsih, Lintje Hutahaean, Dwi Priyanto, Nyak Ilham, Suharyon Suharyon, Herdis Herdis, Yeni Widiawati, Maureen Chrisye Hadiatry, Sara Sorayya Ermuna, Maesti Mardiharini, Dedi Sugandi, Bachtar Bakrie, Wasito Wasito
Abstract The swamp buffalo is a valuable genetic resource and an important source of animal protein in Indonesia. Unfortunately, their population is decreasing due to problems attributed to conventional farming systems. The objective of the study was to examine the sustainability of swamp buffalo farming and identify the attributes that influence its sustainability. Data were collected through a focus group discussion involving experts and business actors in swamp buffalo farming and a survey on buffalo farmers. The study examined 52 attributes within 6 dimensions: ecological, economic, social, technological, institutional, and welfare dimensions. The data were analysed using multidimensional scaling through the Rapid Appraisal for Swamp Buffalo technique. The results of the study demonstrate that swamp buffalo farming in Hulu Sungai Utara (HSU), Hulu Sungai Selatan (HSS), and Hulu Sungai Tengah (HST) districts in South Kalimantan province has sustainability indices of 51.70, 53.13, and 48.87%, respectively. This study identified 12 leverage attributes that are very influential to the sustainability of swamp buffalo farming, i.e., climatic conditions, land suitability, marketing, income from swamp buffalo farming, mutual assistance, education level, processing technology, reproduction technology, the role of local government, capital institutions, programs from central government, and farmers’ welfare. Swamp buffalo farming in HSU and HSS is moderately sustainable, while in HST, it is less sustainable, suggesting improvement in the key factors influencing the sustainability of swamp buffalo farming. This study offers valuable insights for the government in formulating policies and programs for developing swamp buffalo farming and conserving swamp buffalo germplasm.
沼泽水牛是印度尼西亚宝贵的遗传资源和重要的动物蛋白来源。不幸的是,由于传统农业系统的问题,它们的人口正在减少。该研究的目的是检查沼泽水牛养殖的可持续性,并确定影响其可持续性的属性。通过沼泽水牛养殖专家和商业行为者参加的焦点小组讨论和对水牛农民的调查收集了数据。该研究考察了6个维度中的52个属性:生态、经济、社会、技术、制度和福利维度。通过沼泽水牛快速评价技术,采用多维尺度对数据进行分析。研究结果表明,南加里曼丹省Hulu Sungai Utara (HSU)、Hulu Sungai Selatan (HSS)和Hulu Sungai Tengah (HST)地区的沼泽水牛养殖可持续性指数分别为51.70、53.13和48.87%。本研究确定了对沼泽水牛养殖可持续性影响较大的12个杠杆属性,即气候条件、土地适宜性、市场营销、沼泽水牛养殖收入、互助性、教育水平、加工技术、再生产技术、地方政府作用、资本机构、中央政府项目和农民福利。高露江流域和高露江流域的沼泽水牛养殖具有中等可持续性,高露江流域的沼泽水牛养殖具有较低的可持续性,表明影响沼泽水牛养殖可持续性的关键因素有所改善。该研究为政府制定沼泽水牛养殖发展政策和保护沼泽水牛种质资源提供了有价值的见解。
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引用次数: 2
Tailoring business models for small-medium food enterprises in Eastern Africa can drive the commercialization and utilization of vitamin A rich orange-fleshed sweet potato puree 为东非的中小型食品企业量身定制商业模式,可以推动富含维生素A的橙皮甘薯泥的商业化和利用
IF 2.3 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1515/opag-2022-0168
Nyangaresi M. Annette, Tsegaye Makeda, Moyo Mukani, Muzhingi Tawanda
Abstract Orange-fleshed sweet potato (OFSP), a promising crop for alleviating vitamin A deficiency (VAD), can be utilized at home and in commercial food processing as a basic and functional ingredient. The root can be processed into puree that is incorporated in baked and fried products and other products, with a high acceptability rate. When used as a wheat substitute in a bakery, the OFSP puree significantly cuts the production cost. In the last few years and with the intensified promotion of OFSP and OFSP puree products as a sustainable food-based strategy to tackling VAD at the population level, many small-medium food enterprises (SMEs) in the Eastern Africa Region (EAR) have shown great interest in utilizing the OFSP puree in their commercial product lines. However, the OFSP and OFSP puree value chain for commercial usage is still underdeveloped poising raw material supply challenges. In addition, the SMEs are early-stage businesses lacking the capacity and proper business models to propel them to sustainably venture into OFSP processing. As such, there is a need to engage and support SMEs in tailoring business models suitable for their scaling needs along the OFSP value chain to make available in the market, affordable nutrient-dense OFSP-puree products. This article presents the approach that was used to offer tailored Business Development Services (BDS) for selected SMEs in EAR to scale up their capacity to commercialize OFSP puree and puree-based products. The BDS designed and supported six integrated modules: business profile development, business model canvas, understanding the numbers/finances, OFSP value chain analysis, SWOT analysis, and growth plan, to develop and document individual business capacities and aspirations. The SMEs identified numerous opportunities and entry points for OFSP value chain development and expansion through the BDS depending on their business needs.
摘要橙肉红薯(OFSP)是一种很有前途的缓解维生素a缺乏症(VAD)的作物,可作为一种基本的功能性成分在家庭和商业食品加工中使用。根可以加工成果泥,加入烘焙、油炸产品和其他产品中,具有很高的可接受率。当在面包店用作小麦替代品时,OFSP果泥显著降低了生产成本。在过去几年中,随着OFSP和OFSP果泥产品作为在人口层面解决VAD的可持续食品战略的大力推广,东非地区的许多中小型食品企业对在其商业产品线中使用OFSP果浆表现出了极大的兴趣。然而,用于商业用途的OFSP和OFSP纯价值链仍然不发达,这给原材料供应带来了挑战。此外,中小企业是早期企业,缺乏能力和适当的商业模式来推动它们可持续地进入OFSP加工。因此,有必要让中小企业参与并支持他们定制适合其在OFSP价值链上的扩展需求的商业模式,以在市场上提供价格合理、营养丰富的OFSP纯产品。本文介绍了为EAR中选定的中小企业提供量身定制的业务发展服务(BDS)的方法,以提高其将OFSP纯产品和基于纯产品商业化的能力。BDS设计并支持六个集成模块:业务概况开发、业务模型画布、了解数字/财务、OFSP价值链分析、SWOT分析和增长计划,以发展和记录个人业务能力和愿望。中小企业根据其业务需求,通过BDS确定了OFSP价值链发展和扩张的众多机会和切入点。
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引用次数: 0
Optimization of extraction using surface response methodology and quantification of cannabinoids in female inflorescences of marijuana (Cannabis sativa L.) at three altitudinal floors of Peru 表面响应法优化秘鲁3个海拔层大麻(Cannabis sativa L.)雌花花序中大麻素的提取及含量测定
IF 2.3 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1515/opag-2022-0186
Clara Espinoza-Silva, Erika Pascual, Yacnehs Delgadillo, Omar R. Flores, Luis M. Artica, Doris Marmolejo, Lilian Baños-Medina
Abstract The aim of this study was to extract and quantify cannabinoids from female inflorescences of Cannabis sativa L. from three altitudinal floors of Peru, by optimizing the amplitude, time, and methanol concentration in the ultrasound-assisted extraction required to maximize cannabidiol (CBD), delta-9-tetrahydrocannabinol (Δ9-THC) content, and yields. Optimal extraction conditions were determined by response surface and the central composite design was used. The quadratic model was adequate for yield, Δ9-THC, and CBD with R 2 values of 0.998, 0.985, and 0.991 respectively. Optimal conditions were 99% radiation amplitude, 20 min extraction time, and 96% ethanol concentration. The optimized extract of C. sativa L. inflorescences had a yield of 24.12%, 0.62% CBD, and 5.973% THC. The content of cannabinoids studied in the Junín Region at altitudes between 2,070 and 3,274 m above sea level (m asl) had a CBD content between 0.1 and 0.4%, THC between 2.2 and 6%, and yield of 10–24%; in the Ayacucho region at an altitude of 2,627 m asl the CBD content was between 0.62 and 0.65%, THC was 6.21–6.72%, and yield of 23.8–24%; and in the Huánuco region at altitude of 660–711 m asl it had a CBD content between 0.55 and 0.65%, THC from 8.11 to 8.92%, and yield from 24.3 to 29.7%. It was concluded from the present work that the parameters such as amplitude, time, and solvent directly influence the extraction yield, in the same way the altitude influences the content of cannabinoids, being lower yields at higher planting altitude.
摘要本研究的目的是通过优化超声辅助提取大麻二酚(CBD)、δ -9-四氢大麻酚(Δ9-THC)含量和得率所需的振幅、时间和甲醇浓度,从秘鲁三个垂直楼层的大麻(Cannabis sativa L.)雌花花序中提取并定量大麻素。通过响应面法确定最佳提取条件,并采用中心组合设计。二次模型对产量、Δ9-THC和CBD均适用,r2值分别为0.998、0.985和0.991。最佳提取条件为:辐射振幅99%,提取时间20 min,乙醇浓度96%。优化后的红花提取物的CBD提取率为24.12%,THC提取率为0.62%,THC提取率为5.973%。在海拔2070 ~ 3274 m (m asl)的Junín地区所研究的大麻素含量,CBD含量为0.1 ~ 0.4%,THC含量为2.2 ~ 6%,产量为10 ~ 24%;在海拔2627 m的阿亚库乔地区,CBD含量为0.62 ~ 0.65%,四氢大麻酚含量为6.21 ~ 6.72%,产量为23.8 ~ 24%;在海拔660 ~ 711 m的Huánuco地区,CBD含量为0.55 ~ 0.65%,THC含量为8.11% ~ 8.92%,产量为24.3% ~ 29.7%。结果表明,振幅、时间、溶剂等参数直接影响大麻素提取得率,海拔对大麻素含量也有影响,海拔越高大麻素产量越低。
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引用次数: 0
Indicators of swamp buffalo business sustainability using partial least squares structural equation modelling 使用偏最小二乘结构方程建模的沼泽水牛业务可持续性指标
IF 2.3 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1515/opag-2022-0216
N. Widaningsih, B. Hartono, H. Utami, E. Rohaeni, E. Gunawan
Abstract The objective of this study is to analyse the effect of swamp buffalo (Bubalus bubalis carabauesis) sustainability indicators and to examine the relationship between these. The research was conducted in the central area for the development of swamp buffalo in South Kalimantan, namely, Hulu Sungai Utara Regency, in July 2022 with the participation of 145 farmers, who were identified using the multistage sampling method. There were 53 indicators with 9 latent variables, statistically modelled using the partial least squares structural equation modelling method, with evaluation of the outer and inner models. The results show that the information system and economy had a significant effect on human resources (HR), which in turn had a significant effect on the financial and business sustainability of swamp buffalo. The influence of information systems on resources was positive, while economic indicators had a negative influence. HR had a positive relationship with the financial and business sustainability of swamp buffalo. The sustainability indicators in swamp buffalo farming are complex and vary from region to region. Based on the study results, it is expected that the government as policymakers, especially the government of Hulu Sungai Utara Regency, South Kalimantan, pay special attention to the aspects of information systems, the economy, HR, and the financial and business sustainability of swamp buffalo, while also considering other indicators such as ecological, sociocultural, technological, and institutional ones.
摘要本研究的目的是分析沼泽水牛(Bubalus bubalis carabauesis)可持续性指标的影响,并检验它们之间的关系。这项研究于2022年7月在南加里曼丹沼泽水牛发展的中心地区,即Hulu Sungai Utara Regency进行,145名农民参与了研究,他们是使用多级抽样方法确定的。共有53个指标,包含9个潜在变量,使用偏最小二乘结构方程建模方法进行统计建模,并对外部和内部模型进行评估。结果表明,信息系统和经济对人力资源(HR)有显著影响,而人力资源又对沼泽水牛的财务和业务可持续性有显著影响。信息系统对资源的影响是积极的,而经济指标则有负面影响。人力资源与沼泽水牛的财务和业务可持续性呈正相关。沼泽水牛养殖的可持续性指标很复杂,并且因地区而异。根据研究结果,预计政府作为决策者,特别是南加里曼丹Hulu Sungai Utara Regency政府,将特别关注沼泽水牛的信息系统、经济、人力资源以及财务和商业可持续性等方面,同时考虑生态、社会文化、技术、,以及体制性的。
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引用次数: 1
Comparison of three wine routes’ realities in Central Portugal 葡萄牙中部三条葡萄酒路线的现实比较
IF 2.3 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1515/opag-2022-0201
Diana Cunha, Maria Lúcia Pato, E. Kastenholz, C. Barroco
Abstract Wine tourism (WT) is an important area of special-interest tourism in Portugal, and represents an increasingly significant component of regional development. In a more conservative approach, WT has been described as visiting vineyards, wineries, and engaging in wine-related activities. However, this perspective has been broadened, taking advantage of all the potential of the specific destination’s terroir (nature/landscape, tangible and intangible cultural heritage, etc.). Wine routes make the connection between wine and tourism in a specific region and intend to boost wine tourism by promoting collaboration between different stakeholders. Different routes present distinct approaches to WT, within diverse regional contexts, and different ways of collaboration within the wine route. This study compares three wine routes at different stages of development, located in a rural periphery – in the central region of Portugal – Bairrada, Dão, and Beira Interior, considering both context data and information collected in 113 interviews conducted with diverse wine tourism agents from these routes. Besides a brief characterization of the three routes, the main results indicate supplier’s preference for terroir routes instead of wine routes and show the importance of gender, age, and education level for the collaborative work between stakeholders. These aspects and their contribution to the development of WT routes are discussed. Some questions that additional studies may help answering are also reflected.
摘要葡萄酒旅游(WT)是葡萄牙一个重要的特殊旅游领域,也是区域发展的重要组成部分。在一种更保守的方法中,WT被描述为参观葡萄园、酒厂和从事葡萄酒相关活动。然而,这一视角已经被拓宽,利用了特定目的地风土的所有潜力(自然/景观、有形和非物质文化遗产等)。葡萄酒路线将葡萄酒与特定地区的旅游业联系起来,并打算通过促进不同利益相关者之间的合作来促进葡萄酒旅游业。在不同的地区背景下,不同的路线提供了不同的WT方法,以及葡萄酒路线中的不同合作方式。这项研究比较了三条处于不同发展阶段的葡萄酒路线,它们位于葡萄牙中部地区的农村边缘地区——拜拉达、Dão和贝拉内政部,同时考虑了背景数据和对这些路线的不同葡萄酒旅游代理商进行的113次采访中收集的信息。除了对这三条路线的简要描述外,主要结果表明供应商更喜欢风土路线而不是葡萄酒路线,并表明性别、年龄和教育水平对利益相关者之间合作的重要性。讨论了这些方面及其对WT路线发展的贡献。一些额外研究可能有助于回答的问题也得到了反映。
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引用次数: 0
How intergenerational farmers negotiate their identity in the era of Agriculture 4.0: A multiple-case study in Indonesia 在农业4.0时代,代际农民如何协商他们的身份:印度尼西亚的多案例研究
IF 2.3 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1515/opag-2022-0219
E. Widiyanti, R. Karsidi, M. Wijaya, P. Utari
Abstract Identity has become a fascinating object of exploration in various aspects of life and work, including farming. Many studies have examined the extent to which farmers reconstruct their identities, and only a limited number have evaluated the forms of negotiation from a communication perspective. Therefore, herein, we addressed this gap by analysing the identity challenges experienced by farmers in the agricultural era 4.0. A comprehensive farmer identity negotiation model was developed by conducting a multi-case study involving millennial and Generation X farmers from different regions in Indonesia. Data were collected through in-depth interviews with 16 farmers who have embraced Agriculture 4.0 across five regencies in Indonesia. The results showed that farmer identity is maintained and built through various processes, including education, outreach, affiliation, and social networking. Farmer identity negotiation also involves self-preparedness, such as developing communication competence and receiving environmental support through social connections, media, and access to information. The process of farmer identity negotiation ultimately leads to the affirmation of identity, manifesting in changes in social roles, lifestyle changes, and improved farming quality. The advent of Agriculture Revolution 4.0 has necessitated the availability of innovative information, provided access to information and communication technology, and spaces for farmer communities to improve their farming competence.
身份已经成为生活和工作的各个方面,包括农业,一个迷人的探索对象。许多研究考察了农民在多大程度上重建了他们的身份,只有少数研究从沟通的角度评估了谈判的形式。因此,在此,我们通过分析农业4.0时代农民所经历的身份挑战来解决这一差距。通过对印度尼西亚不同地区的千禧一代和X一代农民进行多案例研究,开发了一个全面的农民身份谈判模型。通过对印度尼西亚5个县的16名接受农业4.0的农民进行深入访谈,收集了数据。结果表明,农民身份是通过教育、外联、隶属关系和社会网络等多种过程来维持和建立的。农民身份谈判还涉及自我准备,如发展沟通能力,并通过社会关系、媒体和信息获取获得环境支持。农民身份协商的过程最终导致身份的肯定,表现为社会角色的改变、生活方式的改变和农耕质量的提高。农业4.0革命的到来使得创新信息的可用性成为必要,为农民社区提供了获取信息和通信技术的途径和空间,以提高他们的农业能力。
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引用次数: 0
Physicochemical and microbiological characteristics of various stem bark extracts of Hopea beccariana Burck potential as natural preservatives of coconut sap 柞蚕不同茎皮提取物作为椰汁天然防腐剂的理化和微生物学特性研究
IF 2.3 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1515/opag-2022-0175
D. Raharjo, M. Z. Zaman, D. Praseptiangga, A. Yunus
Abstract The bark of Hopea beccariana Burck is used to prevent damage to coconut sap. The purpose of this study was to determine the physicochemical and microbiological characteristics of various extracts of the bark of H. beccariana Burck as potential natural preservatives for coconut sap. The bark was extracted by maceration method for 24 h assisted by stirring using ethanol, methanol, n-hexane, and water at 60°C as solvent. The type of solvent used for extraction had a significant effect on the yield, total phenolic, total flavonoid, antioxidant activity, toxicity, and diameter of the inhibition zone against Lactobacillus plantarum. The highest yield was found in methanol extract at 22.34%, the highest total phenolic content was found in ethanol extract of 53.39 mg gallic acid equivalent/g extract, and the highest total flavonoid content was found in the aqueous extract at 60°C of 106.70 mg QE/g extract; all extracts have an IC50 value of 80.28–91.80 ppm (very strong antioxidant) and ascorbic acid of 5.78 ppm. Methanol extract is classified as very toxic with an LC50 of 38.21 ppm. The dominant compounds produced by gas chromatography–mass spectrometer were hexanedioic acid, bis(2-ethylhexyl) ester; ethyl oleate; 9-octadecenoic acid (Z)-, ethyl ester; and hexadecanoic acid, ethyl ester. The diameter of the inhibition zone for Saccharomyces cerevisiae was 13.50–14.72 mm, L. plantarum was 10.31–17.72 mm, and Leuconostoc mesenteroides was 13.25–18.06 mm. All extracts had minimum inhibitory concentration values of 2.5 mg/mL and minimum bactericidal concentration values of 2.5 mg/mL for n-hexane extract and 60°C water, while ethanol and methanol extracts had MBC values >50 mg/mL.
摘要为了研究槐皮对椰汁的防腐作用,以槐皮为原料,采用浸渍法提取槐皮24 h,以乙醇、甲醇、正己烷和水为溶剂,在60℃下搅拌,研究槐皮各提取物作为椰汁天然防腐剂的理化和微生物学特性。提取溶剂类型对植物乳杆菌的得率、总酚、总黄酮、抗氧化活性、毒性和抑菌带直径均有显著影响。乙醇提取物的总酚含量为53.39 mg没食子酸当量/g,总黄酮含量为106.70 mg QE/g, 60℃水溶液提取物的总黄酮含量最高;所有提取物的IC50值为80.28-91.80 ppm(非常强的抗氧化剂),抗坏血酸为5.78 ppm。甲醇提取物的LC50为38.21 ppm,属于剧毒。气相色谱-质谱联用分析得到的优势化合物为己二酸双(2-乙基己基)酯;油酸乙酯;9-十八烯酸乙酯;十六烷酸,乙酯。抑菌带直径为13.50 ~ 14.72 mm,植物乳杆菌为10.31 ~ 17.72 mm,肠系膜白僵菌为13.25 ~ 18.06 mm。正己烷提取物和60°C水的最低抑菌浓度为2.5 mg/mL,最低杀菌浓度为2.5 mg/mL,乙醇和甲醇提取物的MBC值为50 mg/mL。
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引用次数: 0
Effect of short-term grazing exclusion on herbage species composition, dry matter productivity, and chemical composition of subtropical grasslands 短期禁牧对亚热带草原牧草物种组成、干物质生产力和化学成分的影响
IF 2.3 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-01-01 DOI: 10.1515/opag-2022-0223
S. Barsila, Mahendra Singh Dhami, Bijay Kumar Shrestha, Luma Nidhi Pandey
Abstract Grazing exclusion (GE) is a useful management technique for restoring degraded grasslands. The herbage mass productivity and chemical makeup in the grazing-excluded subtropical grassland environment has, however, received little attention. A subtropical riverine grassland was selected to determine the effect of GE on herbage mass productivity and chemical composition in Nepal. In three successive harvesting times from September to November 2020, the herbage was sampled along the six randomly selected transects of 100 m length and at two treatments (GA: Grazing-allowed and GE: Grazing-excluded plots) at three different times of harvest from a 1,000-ha grassland. A total of 108 herbage cut samples were collected from the individual 1 m × 1 m quadrats at the three harvests, respectively, from the GA and GE plots. Fences were used to maintain the GE plots to avoid grazing to prevent the vegetation altered by grazing. Day before herbage sampling, the functional groups, cover-abundance within the sampling quadrats were investigated. By cutting the fresh herbage 5 cm above the ground and subjecting it to oven drying for laboratory examination, the herbage mass productivity within each quadrat was measured. Using established laboratory procedures, the chemical analysis of herbage was evaluated for its proximate, fibre, and mineral contents. The results of the study demonstrated that GE significantly increased grass species than other-forbs, other-graminoids, and legumes, respectively, and increased dry matter productivity, which could be seen by an increase in leaf stem ratio, tiller productivity, increased coarseness (fibrous content), total ash, calcium (Ca), and phosphorus (P), but with a decreased nonstructural carbohydrate, and the concentrations of ether extract and crude protein. Research results also confirmed that GE increases herbages’ fibrousness and productivity, though the herbage quality, intake, and digestibility decline. It further demonstrates that grazing is a crucial biological component for maintaining pasture quality in subtropical grasslands and that managing grasslands through livestock grazing would make grasslands more stable and nutrient-enriched. The findings of this study can be useful in the long-term monitoring of grazing livestock in the subtropical grasslands when considering further investigations with the multiple factors in future.
禁牧是恢复退化草地的一项有效管理技术。然而,对亚热带草地放牧环境下牧草质量生产力和化学组成的研究却很少。在尼泊尔选取了一片亚热带河流草地,研究了转基因对牧草质量生产力和化学成分的影响。在2020年9月至11月的三个连续收获期,在1000公顷草地上随机选择6个长度为100 m的样带,在三个不同的收获时间进行两种处理(GA:允许放牧和GE:不允许放牧)的牧草取样。三次采收时,在遗传和转基因样地的1 m × 1 m样地共采集108份割草样品。采用围栏保护转基因样地避免放牧,防止放牧对植被的改变。采样前一天,对样方内的功能基团、覆盖度进行调查。将新鲜牧草在离地5cm处割下,经烘箱烘干后进行实验室检验,测定每个样方内牧草的质量生产率。利用已建立的实验室程序,对牧草的化学分析进行了评估,包括其近似物、纤维和矿物质含量。结果表明,与其他草本植物、其他禾草类植物和豆科植物相比,转基因显著增加了禾草种类,并显著提高了干物质生产力,表现为叶茎比、分蘖生产力、粗度(纤维含量)、总灰分、钙(Ca)和磷(P)增加,但非结构碳水化合物、粗脂肪和粗蛋白质浓度降低。研究结果还证实,转基因提高了牧草的纤维性和生产力,但降低了牧草的品质、采食量和消化率。研究进一步表明,放牧是维持亚热带草原牧草质量的重要生物成分,通过放牧对草原进行管理可以使草原更加稳定和营养丰富。本研究结果可为亚热带草原放牧牲畜的长期监测提供参考,并可在今后的多因素调查中进一步研究。
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