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Live imaging of excitable axonal microdomains in ankyrin-G-GFP mice. ankyrin-G-GFP小鼠可兴奋轴突微域的实时成像。
4区 管理学 Q2 HOSPITALITY, LEISURE, SPORT & TOURISM Pub Date : 2024-06-19 DOI: 10.1101/2023.02.01.525891
Christian Thome, Jan Maximilian Janssen, Seda Karabulut, Claudio Acuna, Elisa D'Este, Stella J Soyka, Konrad Baum, Michael Bock, Nadja Lehmann, Johannes Roos, Nikolas A Stevens, Masashi Hasegawa, Dan A Ganea, Chloé M Benoit, Jan Gründemann, Lia Min, Kalynn M Bird, Christian Schultz, Vann Bennett, Paul M Jenkins, Maren Engelhardt

The axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation of output generation. Recent studies shedding light on AIS function used predominantly post-hoc approaches since no robust murine in vivo live reporters exist. Here, we introduce a reporter line in which the AIS is intrinsically labeled by an ankyrin-G-GFP fusion protein activated by Cre recombinase, tagging the native Ank3 gene. Using confocal, superresolution, and two-photon microscopy as well as whole-cell patch-clamp recordings in vitro, ex vivo, and in vivo, we confirm that the subcellular scaffold of the AIS and electrophysiological parameters of labeled cells remain unchanged. We further uncover rapid AIS remodeling following increased network activity in this model system, as well as highly reproducible in vivo labeling of AIS over weeks. This novel reporter line allows longitudinal studies of AIS modulation and plasticity in vivo in real-time and thus provides a unique approach to study subcellular plasticity in a broad range of applications.

轴突起始节段(AIS)不仅是动作电位的起始部位,也是依赖活动调节输出生成的枢纽。由于没有可靠的小鼠体内活体报告基因,近期揭示 AIS 功能的研究主要采用事后研究的方法。在这里,我们引入了一种报告基因,其中的 AIS 被 Cre 重组酶激活的 ankyrin-G-GFP 融合蛋白内在标记,并标记了原生的 Ank3 基因。利用共焦、超分辨率和双光子显微镜以及体外、体内和体外全细胞膜片钳记录,我们证实 AIS 的亚细胞支架和标记细胞的电生理参数保持不变。我们进一步发现,在这一模型系统中,随着网络活动的增加,AIS 会迅速重塑,而且 AIS 的体内标记可在数周内高度重现。这种新颖的报告基因可以对体内 AIS 的调节和可塑性进行实时纵向研究,从而为研究亚细胞可塑性的广泛应用提供了一种独特的方法。
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引用次数: 0
A pentadic analysis of TikTok marketing in tourism: The case of Penang, Malaysia 旅游业 TikTok 营销的五元分析:马来西亚槟城案例
IF 2.4 4区 管理学 Q2 HOSPITALITY, LEISURE, SPORT & TOURISM Pub Date : 2023-12-16 DOI: 10.1177/14687976231218483
Aaron Tham, Shu-Hsiang (Ava) Chen, Levi Durbidge
Several destinations have jumped on the TikTok bandwagon to explore consumer reactions and motivations towards visit intentions, though these are largely limited to point-in-time surveys. Using the example of Penang, Malaysia, this research contributes to the paucity of knowledge surrounding TikTok in tourism by analysing contents generated from the destination management organisation, travel influencers, and other tourists participating in a TikTok competition for the destination. Drawing from Burke’s Pentadic analysis, the findings reveal the sociological interactions where the performativity of the short videos are staged by different users with the destination as the backdrop. Theoretical and managerial implications are highlighted to inform current and future practices of TikTok (re)presentation in tourism, and how the platform can be used distinctively to socially interact with various target markets and stakeholders.
一些旅游目的地已经加入了 TikTok 的行列,以探索消费者的反应和旅游意向的动机,尽管这些调查主要局限于时间点调查。本研究以马来西亚槟城为例,通过分析目的地管理机构、旅游影响者和其他参与目的地 TikTok 竞赛的游客所产生的内容,为旅游业中有关 TikTok 的知识的匮乏做出了贡献。借鉴伯克的五元分析法,研究结果揭示了不同用户以目的地为背景上演短视频表演的社会学互动。研究强调了理论和管理方面的意义,为旅游业当前和未来的 TikTok(再)展示实践提供了参考,并说明了如何以独特的方式利用该平台与不同的目标市场和利益相关者进行社会互动。
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引用次数: 0
Migrant support volunteer tourism facing crises. Patchwork autoethnographies on Lesvos (Greece) 面临危机的移民支持志愿旅游。莱斯沃斯(希腊)的拼凑自述
IF 2.4 4区 管理学 Q2 HOSPITALITY, LEISURE, SPORT & TOURISM Pub Date : 2023-12-02 DOI: 10.1177/14687976231216127
Giovanna Di Matteo, Luca Daminelli
The island of Lesvos serves as a symbol of migration in the Mediterranean. From 2015 onwards, a steady stream of volunteer tourists began working on the island. Between 2019 and 2022 various ‘crises’ such as the Covid-19 pandemic, the fire at the Moria camp, and the rise of the far right in Greece significantly altered living conditions for both refugees and volunteer workers on the island. The authors employ a comparative patchwork autoethnography to rethink participant observation to fit the pandemic era. Through a situated, relational, and more-than-representational analysis of autoethnographic material and the confrontation between the authors’ experiences, the aim of this paper is to investigate how migrant support volunteer tourism interacts with various simultaneous ‘crises’ ongoing on Lesvos. This led to a reimagining of the way we understand volunteer tourism over its ‘pro vulnerable’ approach.
莱斯沃斯岛是地中海地区移民的象征。从2015年开始,源源不断的志愿者游客开始在岛上工作。2019年至2022年期间,各种“危机”,如新冠肺炎大流行、莫里亚难民营大火以及希腊极右翼势力的崛起,极大地改变了岛上难民和志愿者的生活条件。作者采用了一种比较拼凑的自我民族志来重新思考参与者的观察,以适应大流行时代。通过对自身民族志材料的情境、关系和非代表性分析,以及作者经验之间的冲突,本文的目的是研究移民支持志愿者旅游如何与莱斯沃斯岛上正在进行的各种同时发生的“危机”相互作用。这让我们重新思考志愿者旅游的方式,而不是“支持弱势群体”的方式。
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引用次数: 0
(M)apping film in Scotland: Film tour maps, apps and ‘real’ engagements with virtual place (M)apping film in Scotland:电影游览图、应用程序和与虚拟场所的 "真实 "接触
IF 2.4 4区 管理学 Q2 HOSPITALITY, LEISURE, SPORT & TOURISM Pub Date : 2023-11-24 DOI: 10.1177/14687976231206851
R. Schiavone, Amanda Brandellero
This article presents an analysis of film tour apps and maps, exploring the different functionalities they offer, how these functionalities contribute to shaping engagements with place in Edinburgh, Scotland, and the specific role of film in this process. We find that while they possess similar functionalities to more general tourism apps – for example, providing information and offering travel advice – film tour apps and maps also connect the real world to the ‘reel’ world through games, active engagement, and prosumerism. The specific functionalities of film media apps, we argue, have ramifications for film tourism experiences, potentially extending the touristic experience of a destination to a multi-mediated, multi-layered experience of a ‘patchwork’ place through the lens of film. Additionally, functionalities of film tour apps facilitate new types of behaviours and interactions with place.
本文对电影旅游应用程序和地图进行了分析,探讨了它们所提供的不同功能,这些功能如何有助于形成与苏格兰爱丁堡地方的互动,以及电影在这一过程中的具体作用。我们发现,虽然它们具有与一般旅游应用程序类似的功能(例如提供信息和旅游建议),但电影旅游应用程序和地图还通过游戏、主动参与和亲消费主义将现实世界与 "卷轴 "世界联系起来。我们认为,电影媒体应用程序的特殊功能会对电影旅游体验产生影响,有可能将目的地的旅游体验扩展为通过电影镜头对 "拼凑 "地点的多媒介、多层次体验。此外,电影旅游应用程序的功能还能促进新型行为以及与地方的互动。
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引用次数: 0
‘If not now, then never’: Conceptualising the grad trip “如果不是现在,那就永远不会”:构想毕业旅行
4区 管理学 Q2 HOSPITALITY, LEISURE, SPORT & TOURISM Pub Date : 2023-10-12 DOI: 10.1177/14687976231203751
Yinn Shan Cheong, Harng Luh Sin, Tou Chuang Chang
The grad trip entails travelling overseas with one’s closest group of friends to commemorate the completion of tertiary and formal education and is bookended by the carefree freedom in one’s youth and the commencement of an adulthood defined by formal employment. This article conceptualises the grad trip as a phenomenon in youth tourism, based on empirical study of how it is experienced in Singapore. Our conceptualisation of the timing and duration that defines a grad trip demonstrates the dominance of time productivity reflective of Singapore’s work culture. Drawing on a precedence of studies based in the Western concept of the gap year, we reveal commonalities in employment society’s influence on time for youth tourism. While the gap year seeks to maximise the length of time offered by a ‘gap’, the grad trip contrarily minimises the culturally perceived wastefulness of a prolonged break.
毕业旅行需要和最亲密的朋友一起到海外旅行,以纪念完成高等教育和正规教育,结束于青年时期无忧无虑的自由和正式就业所定义的成年期的开始。本文基于对新加坡的经验研究,将毕业生旅行概念化为青年旅游中的一种现象。我们对毕业生旅行的时间和持续时间的概念化表明了时间生产力的主导地位,反映了新加坡的工作文化。借鉴西方间隔年概念的研究先例,我们揭示了就业社会对青年旅游时间影响的共性。间隔年旨在最大限度地延长“间隔”所提供的时间,而毕业生旅行则相反,最大限度地减少了文化上认为的长时间休假的浪费。
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引用次数: 0
‘An Gorta Mor (The Great Hunger): Passage to India’ – Exploring genealogy and roots tourism through documentary filmmaking “An Gorta Mor(大饥饿):印度之旅”-通过纪录片制作探索家谱和根源旅游
4区 管理学 Q2 HOSPITALITY, LEISURE, SPORT & TOURISM Pub Date : 2023-10-11 DOI: 10.1177/14687976231203749
Ian Michael, Paolo Mura
This article presents a documentary film produced and enacted by one of the authors as a way of knowing, constructing and representing a specific form of tourist consumption, namely genealogy tourism. The documentary ( https://vimeo.com/825001015?share=copy ), entitled ‘An Gorta Mor’ (Gaelic words that translate to ‘The Great Hunger’, the Irish famine that occurred from 1845 to 1852), functions as both a vehicle to produce and present the ethnographic fieldwork conducted by one of the authors to trace back his ancestral roots and the empirical material to be analysed. The documentary unveils two emergent themes. The first is that genealogy tourism involves multiple fluid places and transnational identities that question ‘fixed’ notions of ‘motherland’, ‘home’ and ‘family’. The second refers to roots tourism as a vehicle to propel bonding and bridging social capital. Methodologically, as a form of arts-based research, this work promotes embodied alternative ways of knowing tourist realities and selves.
本文通过作者之一制作并制作的一部纪录片,来认识、建构和呈现一种特殊的旅游消费形式——家谱旅游。这部名为“An Gorta Mor”(盖尔语,意为“大饥荒”,1845年至1852年发生的爱尔兰饥荒)的纪录片(https://vimeo.com/825001015?share=copy),作为一种工具,展示了作者之一进行的民族志田野调查,以追溯他的祖先根源和要分析的经验材料。这部纪录片揭示了两个新兴的主题。首先,家谱旅游涉及多个流动的地方和跨国身份,这对“祖国”、“家”和“家庭”的“固定”概念提出了质疑。第二种是将根部旅游作为一种促进联系和弥合社会资本的工具。在方法上,作为一种基于艺术的研究形式,这项工作促进了了解游客现实和自我的具体替代方法。
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引用次数: 0
Changing roles of tour guides: From “agent to serve” to “agent of change” 导游角色的转变:从“服务的代理人”到“变革的代理人”
4区 管理学 Q2 HOSPITALITY, LEISURE, SPORT & TOURISM Pub Date : 2023-09-27 DOI: 10.1177/14687976231200909
Lianping Ren, Cora Un In Wong, Caiwei Ma, Yanping Feng
The guided tour industry has undergone drastic changes in China along with the socio-economic, demographic, and technological developments of the past decades, leading to new demands on the tour guide profession. The COVID-19 pandemic has further accelerated the changes. Yet little is known regarding how the tour guides’ roles have evolved. This study aims to fill this gap through exploring new roles and services undertaken by tour guides, drawing on qualitative data (in-depth interviews) from both tour guides’ and tourists’ recent guided tour experience in China. The findings demonstrate a continuum of roles of the guides, ranging from “the serving roles” to being “the ones who facilitate changes.” Five main driving forces were identified, namely: the tourists’ customization needs, demographic change, rapid ICT development, increased special interests, and the pandemic and tourists’ favor in remote destinations. Instead of expecting serving roles only, the Chinese tourists now welcome roles that can foster positive changes, reflecting Chinese tourists’ motivational shift to having eudaimonic experience through traveling.
在过去的几十年里,随着社会经济、人口和技术的发展,中国的导游行业发生了巨大的变化,对导游职业提出了新的要求。2019冠状病毒病大流行进一步加速了这些变化。然而,人们对导游的角色是如何演变的知之甚少。本研究旨在通过探索导游的新角色和服务,利用导游和游客最近在中国的导游经历的定性数据(深度访谈)来填补这一空白。研究结果表明,向导的角色是连续的,从“服务角色”到“促进变革的角色”。确定了五个主要驱动力,即:游客的定制需求、人口变化、信息通信技术的快速发展、特殊兴趣的增加以及疫情和游客对偏远目的地的青睐。中国游客不再仅仅期待服务角色,而是欢迎能够促进积极变化的角色,这反映出中国游客的动机转变为通过旅行获得幸福的体验。
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引用次数: 0
‘If they touch our cloudberries, that means war’: Rural liveability and acceptance of environmental impacts from event tourism “如果他们碰了我们的云莓,那就意味着战争”:农村宜居性和对活动旅游对环境影响的接受
4区 管理学 Q2 HOSPITALITY, LEISURE, SPORT & TOURISM Pub Date : 2023-09-26 DOI: 10.1177/14687976231200902
Axel Eriksson
Through the lens of liveability and Buen Vivir, I explore how local actors form their acceptance of the physical impact on nature caused by a trail marathon in north-central Sweden, particularly given trail and soil erosion. With a qualitative multi-method research approach, the findings reveal that the local actors minimise the impacts by getting involved in various activities both inside and outside the event. Different knowledge and practices foster sustainability and create acceptance. While the growth of tourism creates unease and feelings of inadequate control, this event is seen as a distinct phenomenon. I show that liveability goes beyond perception and requires integration of the local environment into local practices. Current acceptance may however be eroded if more or larger events occur in the future. Policies and planning must therefore acknowledge and incorporate these local practices to create sustainability.
通过宜居性和Buen Vivir的视角,我探索了当地参与者如何接受瑞典中北部越野跑马拉松对自然造成的物理影响,特别是考虑到赛道和土壤侵蚀。通过定性的多方法研究方法,研究结果表明,当地参与者通过参与活动内外的各种活动来最大限度地减少影响。不同的知识和实践促进可持续性并创造接受度。虽然旅游业的增长造成了不安和控制不足的感觉,但这一事件被视为一种独特的现象。我展示了宜居性超越了感知,需要将当地环境融入当地实践。然而,如果将来发生更多或更大的事件,当前的接受度可能会受到影响。因此,政策和规划必须承认并纳入这些地方做法,以创造可持续性。
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引用次数: 0
Tourism mobilities, tourist performances: Editors-in-chief’s notes 旅游交通,旅游表演:总编笔记
4区 管理学 Q2 HOSPITALITY, LEISURE, SPORT & TOURISM Pub Date : 2023-09-01 DOI: 10.1177/14687976231200699
Chin-Ee Ong, Susan Frohlick
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引用次数: 0
Serious leisure imaginaries: The construction of tourists, locals and localities in scuba diving tourism 严肃休闲想象:水肺潜水旅游中游客、当地人和地方的建构
IF 2.4 4区 管理学 Q2 HOSPITALITY, LEISURE, SPORT & TOURISM Pub Date : 2023-08-10 DOI: 10.1177/14687976231193110
Hans Tunestad
Scuba diving is a global leisure activity today often undertaken as a form of tourism, counting millions of practitioners. Drawing on my experience of diving in different parts of the world, as well as on various forms of documentation, this article focuses on the structural characteristics of this form of purposeful tourism. As a sport, scuba diving follows certain routines and regulations and can thus be seen as a form of serious leisure; that is, leisure involving plans and demands. As a form of tourism, it is a particular way of imagining the world, based on a specific form of cartography and mass mediated images and stories of the vast blue and its inhabitants. My argument is that when undertaking scuba as a form of tourism, whether guiding or being guided, divers submit to the structures of both the sport and the touring. These then determine both how they dive from a technical point of view and how they come to think about diving and indeed, in a sense, the world. Divers are thus guided by ‘serious leisure imaginaries’. Altogether, scuba diving tourism here emerges as a transformative social force where tourists, locals and localities are constructed simultaneously in a dynamic interplay.
如今,水肺潜水是一项全球性的休闲活动,通常作为一种旅游形式进行,有数百万从业者。根据我在世界各地潜水的经验,以及各种形式的文献,本文重点介绍了这种有目的旅游形式的结构特征。作为一项运动,水肺潜水遵循某些常规和规定,因此可以被视为一种严肃的休闲形式;也就是说,休闲包括计划和需求。作为一种旅游形式,它是一种想象世界的特殊方式,基于一种特定的制图形式和大众媒介的广阔蓝色及其居民的图像和故事。我的论点是,当潜水作为一种旅游形式时,无论是在导游还是在导游的带领下,潜水员都要服从运动和旅游的结构。然后,这些决定了他们从技术角度如何潜水,以及他们如何思考潜水,从某种意义上说,甚至是世界。因此,潜水员被“严肃的休闲想象”所引导。总之,这里的水肺潜水旅游是一种变革性的社会力量,游客、当地人和当地人在动态的相互作用中同时构建。
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引用次数: 0
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Tourist Studies
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