Spatial thinking skills are integral to understanding human nature and driving technological advancements. This research examined 41 pre-service teachers’ spatial thinking skills and spatial anxiety over four years using a longitudinal approach. The data were collected through the Santa Barbara Sense of Direction Scale (SBSOD), the Spatial Anxiety Scale (SAS), and a personal information form. Analyses were conducted using ANOVA, t-tests, correlation analysis, and two-level hierarchical linear modeling (HLM). ANOVA and temporal trends from HLM revealed that pre-service teachers’ environmental spatial skills and spatial anxiety did not follow a linear progression over the four years; however, environmental spatial skills peaked, and spatial anxiety was lowest, during the second year. Furthermore, HLM analyses identified spatial anxiety, preschool education background, GPA, and gender as significant predictors of environmental spatial thinking skills. Additionally, environmental spatial thinking skills, preschool education, and gender emerged as the key predictors of spatial anxiety. Given the absence of linear development, the research recommends revising the curriculum to adopt a progressive, reflective, and application-oriented structure that supports sustained growth. Furthermore, future research could prioritize affective and experiential factors, such as spatial anxiety and preschool education, over biological factors, such as gender, to better understand the mechanisms underlying differences in spatial thinking skills.
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