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Biomimetic 3D bioprinting approaches to engineer the tumor microenvironment 利用仿生3D生物打印技术设计肿瘤微环境
3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-08-22 DOI: 10.36922/ijb.1022
Fabiano Bini, Salvatore D’Alessandro, Tarun Agarwal, Daniele Marciano, Serena Duchi, Enrico Lucarelli, Giancarlo Ruocco, Franco Marinozzi, Gianluca Cidonio
With the increasing incidence and mortality rates, cancer remains a major health challenge in the world. Despite advances in therapies and clinical programs, the efficacy of anti-cancer drugs often fails to translate from pre-clinical models to patient clinical trials. To date, pre-clinical cancer models, including two-dimensional cell cultures and animal models, have limited versatility and accuracy in recapitulating the complexity of human cancer. To address these limitations, a growing focus has fostered the development of three-dimensional (3D) tumor models that closely resemble the in vivo tumor microenvironment and heterogeneity. Recent efforts have leveraged bioengineering technologies, such as biofabrication, to engineer new platforms that mimic healthy and diseased organs, aiming to overcome the shortcomings of conventional models, such as for musculoskeletal tissues. Notably, 3D bioprinting has emerged as a powerful tool in cancer research, offering precise control over cell and biomaterial deposition to fabricate architecturally complex and reproducible functional models. The following review underscores the urgent need for more accurate and relevant 3D tumor models, highlighting the advantages of the use of biofabrication approaches to engineer new biomimetics platforms. We provide an updated discussion on the role of bioengineering technologies in cancer research and modeling with particular focus on 3D bioprinting platforms, as well as a close view on biomaterial inks and 3D bioprinting technologies employed in cancer modeling. Further insights into the 3D bioprinting tissue-specific modeling panorama are presented in this paper, offering a comprehensive overview of the new possibilities for cancer study and drug discovery.  
随着发病率和死亡率的增加,癌症仍然是世界上一个主要的健康挑战。尽管治疗和临床项目取得了进展,但抗癌药物的疗效往往无法从临床前模型转化为患者临床试验。迄今为止,临床前癌症模型,包括二维细胞培养和动物模型,在概括人类癌症的复杂性方面具有有限的通用性和准确性。为了解决这些局限性,越来越多的人关注于促进三维(3D)肿瘤模型的发展,这些模型与体内肿瘤微环境和异质性非常相似。最近的努力利用生物工程技术,如生物制造,来设计模拟健康和患病器官的新平台,旨在克服传统模型(如肌肉骨骼组织)的缺点。值得注意的是,3D生物打印已经成为癌症研究的有力工具,可以精确控制细胞和生物材料沉积,以制造结构复杂且可复制的功能模型。下面的综述强调了迫切需要更准确和相关的3D肿瘤模型,强调了使用生物制造方法来设计新的仿生平台的优势。我们提供了关于生物工程技术在癌症研究和建模中的作用的最新讨论,特别关注3D生物打印平台,以及生物材料墨水和3D生物打印技术在癌症建模中的应用。本文提出了对3D生物打印组织特异性建模全景的进一步见解,全面概述了癌症研究和药物发现的新可能性。,,
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引用次数: 0
3D bioprinting-based single liver tumor spheroid analysis for aflatoxin B1-induced drug-resistant cancer cell 基于3D生物打印的单肝肿瘤球体分析黄曲霉毒素b1诱导的耐药癌细胞
3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-08-18 DOI: 10.36922/ijb.0985
Viet Phuong Cao, Sera Hong, Joon Myong Song
Aflatoxin B1, found in a variety of foods, is a mycotoxin known to cause cancer. Therefore, humans may be exposed to it through their daily diet. In this study, a three-dimensional (3D) tumor spheroid model was developed via 3D bioprinting to examine whether exposure of HepG2 liver tumor spheroids to aflatoxin B1 can increase the population of drug-resistant liver cancer cells in a single tumor spheroid. Two biomarkers, CD133 (prominin-1) and aldehyde dehydrogenase 1 (ALDH1), were used to identify drug-resistant cancer cells formed in the single liver tumor spheroids. The induction of drug-resistant cancer cells in the single tumor spheroids was examined through single spheroid imaging and fluorescence-activated cell sorting (FACS). The increase of drug-resistant cancer cells, which was caused by aflatoxin B1 in a dose-dependent manner, was quantitatively monitored at the single tumor spheroid level using both methods. 3D bioprinting-fabricated single liver tumor spheroid model successfully determined drug-resistant liver cancer cells caused by aflatoxin B1
黄曲霉毒素B1存在于多种食物中,是一种已知会致癌的霉菌毒素。因此,人类可能会通过日常饮食接触到它。本研究通过生物3D打印技术建立了一个三维(3D)肿瘤球体模型,以检验HepG2肝肿瘤球体暴露于黄曲霉毒素B1是否会增加单个肿瘤球体中耐药肝癌细胞的数量。两种生物标志物CD133 (pronin -1)和醛脱氢酶1 (ALDH1)被用来鉴定在单个肝肿瘤球体中形成的耐药癌细胞。通过单球体成像和荧光激活细胞分选(FACS)检测单个肿瘤球体对耐药癌细胞的诱导作用。利用两种方法在单个肿瘤球体水平上定量监测黄曲霉毒素B1引起的耐药癌细胞的增加,其呈剂量依赖性。生物3D打印制备单个肝癌球体模型成功测定黄曲霉毒素B1致肝癌耐药细胞
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引用次数: 0
Melt electrospinning writing PCL scaffolds after alkaline modification with outstanding cytocompatibility and osteoinduction 碱性改性后的熔融静电纺丝书写PCL支架具有良好的细胞相容性和成骨性
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-08-11 DOI: 10.36922/ijb.1071
Yubo Shi, Lei Wang, Liguo Sun, Zhennan Qiu, Xiaoli Qu, Jingyi Dang, Zhao Zhang, Jiankang He, Hongbin Fan
Melt electrospinning writing (MEW) is a promising three-dimensional (3D) printing technology that enables the creation of scaffolds with highly ordered microfibers. Polycaprolactone (PCL) is an ideal material for MEW scaffold fabrication due to its exceptional printability. However, its low cellular affinity can hinder its performance in bone tissue engineering. This study aimed to explore the potential of NaOH treatment as a means of enhancing the cytocompatibility and osteoinductive properties of PCL scaffolds. After modification with a NaOH solution, the physiochemical properties of the MEW PCL scaffold were analyzed. The surface of the scaffold was found to have nanopits and nanogrooves, which differed from the smooth surface of the PCL scaffold. Atomic force microscopy and automatic water contact angle assays revealed an increase in surface roughness and wettability, both of which were found to be beneficial for cell proliferation and adhesion. In vitro experiments demonstrated that the NaOH-treated surface was able to induce osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) via the integrinα2/β1-PI3K-Akt signaling pathway, which had not been previously observed. The study involved implanting PCL scaffold to repair a cranial defect. After 1 and 3 months of implantation, histological analysis and micro-computed tomography scans showed a higher amount of newly formed bone on the NaOH-treated PCL scaffolds compared to the PCL scaffold. The study concluded that NaOH treatment was a simple and effective way to enhance cellular affinity and osteoinductive property of MEW PCL scaffold. This strategy may provide a cost-efficient method for promoting bone regeneration.
熔融静电纺丝书写(MEW)是一种很有前途的三维(3D)打印技术,它可以制造高度有序的微纤维支架。聚己内酯(PCL)是一种理想的材料,由于其特殊的印刷性。然而,其细胞亲和性较低,阻碍了其在骨组织工程中的应用。本研究旨在探讨氢氧化钠作为一种增强PCL支架细胞相容性和骨诱导性能的手段的潜力。用NaOH溶液对其进行改性后,对其理化性能进行了分析。与PCL支架的光滑表面不同,支架表面存在纳米微孔和纳米沟槽。原子力显微镜和自动水接触角分析显示,表面粗糙度和润湿性增加,这两者都有利于细胞增殖和粘附。体外实验表明,naoh处理后的表面能够通过整合素α2/β1-PI3K-Akt信号通路诱导大鼠骨髓间充质干细胞(BMSCs)成骨分化,这是之前未观察到的。该研究涉及植入PCL支架修复颅骨缺损。植入1个月和3个月后,组织学分析和显微计算机断层扫描显示,与PCL支架相比,naoh处理的PCL支架上新形成的骨量更高。本研究认为NaOH处理是一种简单有效的增强MEW PCL支架细胞亲和力和成骨诱导性能的方法。这种策略可能为促进骨再生提供一种经济有效的方法。
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引用次数: 0
3D printing and bioprinting in urology 泌尿外科的3D打印和生物打印
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-08-10 DOI: 10.36922/ijb.0969
Kun Liu, Nan Hu, Zhihai Yu, Xin-Zheng Zhang, Hualin Ma, Huawei Qu, Changshun Ruan
Three-dimensional (3D) printing with highly flexible fabrication offers unlimited possibilities to create complex constructs. With the addition of active substances such as biomaterials, living cells, and growth factors, 3D printing can be upgraded to 3D bioprinting, endowing fabricated constructs with biological functions. Urology, as one of the important branches of clinical medicine, covers a variety of organs in the human body, such as kidneys, bladder, urethra, and prostate. The urological organs are multi-tubular, heterogeneous, and anisotropic, bringing huge challenges to 3D printing and bioprinting. This review aims to summarize the development of 3D printing and bioprinting technologies in urology in the last decade based on the Science Citation Index-Expanded (SCI-E) in the Web of Science Core Collection online database (Clarivate). First, we demonstrate the search strategies for published papers using the keywords such as “3D printing,” “3D bioprinting,” and “urology.” Then, eight common 3D printing technologies were introduced in detail with their characteristics, advantages, and disadvantages. Furthermore, the application of 3D printing in urology was explored, such as the fabrication of diseased organs for doctor–patient communication, surgical planning, clinical teaching, and the creation of customized medical devices. Finally, we discuss the exploration of 3D bioprinting to create in vitro bionic 3D environment models for urology. Overall, 3D printing provides the technical support for urology to better serve patients and aid teaching, and 3D bioprinting enables the clinical applications of fabricated constructs for the replacement and repair of urologically damaged organs in future.
具有高度柔性制造的三维(3D)打印为创建复杂结构提供了无限的可能性。随着生物材料、活细胞、生长因子等活性物质的加入,3D打印可以升级为3D生物打印,使制造的结构物具有生物功能。泌尿外科是临床医学的重要分支之一,涵盖了人体的肾脏、膀胱、尿道、前列腺等多种器官。泌尿系统器官具有多管性、异质性和各向异性,这给3D打印和生物打印带来了巨大的挑战。本文以Web of Science Core Collection在线数据库(Clarivate)中的SCI-E为基础,综述了近十年来3D打印和生物打印技术在泌尿外科领域的发展。首先,我们演示了使用“3D打印”、“3D生物打印”和“泌尿学”等关键词搜索已发表论文的策略。然后,详细介绍了八种常见的3D打印技术,以及它们的特点、优缺点。此外,还探讨了3D打印在泌尿外科的应用,如用于医患交流、手术计划、临床教学的病变器官的制造以及定制医疗设备的创建。最后,我们讨论了3D生物打印在泌尿外科体外仿生3D环境模型中的探索。总体而言,3D打印为泌尿外科更好地服务患者和辅助教学提供了技术支持,3D生物打印为未来泌尿系统损伤器官的替换和修复提供了临床应用。
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引用次数: 0
Rheology-informed hierarchical machine learning model for the prediction of printing resolution in extrusion-based bioprinting 基于流变学的分层机器学习模型用于挤压生物打印中打印分辨率的预测
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-08-09 DOI: 10.36922/ijb.1280
Dageon Oh, M. Shirzad, Min Chang Kim, Eun-Jae Chung, S. Y. Nam
In this study, a rheology-informed hierarchical machine learning (RIHML) model was developed to improve the prediction accuracy of the printing resolution of constructs fabricated by extrusion-based bioprinting. Specifically, the RIHML model, as well as conventional models such as the concentration-dependent model and printing parameter-dependent model, was trained and tested using a small dataset of bioink properties and printing parameters. Interestingly, the results showed that the RIHML model exhibited the lowest error percentage in predicting the printing resolution for different printing parameters such as nozzle velocities and pressures, as well as for different concentrations of the bioink constituents. Besides, the RIHML model could predict the printing resolution with reasonably low errors even when using a new material added to the alginate-based bioink, which is a challenging task for conventional models. Overall, the results indicate that the RIHML model can be a useful tool to predict the printing resolution of extrusion-based bioprinting, and it is versatile and expandable compared to conventional models since the RIHML model can easily generalize and embrace new data.
在本研究中,开发了一种基于流变学的分层机器学习(RIHML)模型,以提高挤压生物打印构建体打印分辨率的预测精度。具体来说,RIHML模型以及传统的模型,如浓度依赖模型和打印参数依赖模型,使用生物墨水特性和打印参数的小数据集进行训练和测试。有趣的是,结果表明,RIHML模型在预测不同打印参数(如喷嘴速度和压力)以及不同浓度的生物墨水成分的打印分辨率时表现出最低的错误率。此外,即使在藻酸盐基生物墨水中添加新材料,RIHML模型也能以相当低的误差预测打印分辨率,这是传统模型的一个挑战。总体而言,结果表明,RIHML模型可以作为预测挤出生物打印分辨率的有用工具,并且与传统模型相比,RIHML模型具有通用性和可扩展性,因为RIHML模型可以很容易地概括和接受新数据。
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引用次数: 0
Development of a 3D-printable matrix using cellulose microfibrils/guar gum-based hydrogels and its post-printing antioxidant activity   利用纤维素微纤维/瓜尔胶基水凝胶开发3d可打印基质及其打印后抗氧化活性
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-08-08 DOI: 10.36922/ijb.0164
Olajide Emmanuel Adedeji, Ju Hyun Min, Gi Eon Park, Hye Jee Kang, Ji-Young Choi, Mariam Omowunmi Aminu, Ocheme Boniface Ocheme, S. Joo, K. Moon, Young Hoon Jung
A biomaterial ink suitable for three-dimensional (3D) printing was developed using cellulose microfibrils (CMFs, 1% w/v) and guar gum (1–7 g/100 mL CMFs), and the post-printing stability and antioxidant functionality of the borax-treated construct were investigated. Rheological analysis, Fourier transform infrared spectrometry, X-ray diffractometry, and scanning electron microscopy revealed the suitability of the two polymers to form an interpenetrating composite hydrogel that would facilitate printability. The produced composite hydrogel showed good structural, morphological, thermal, and textural properties. CMFs with 5% guar gum showing optimal surface properties and rheological properties were printed with the least dimensional errors at 50% infill density, 10 mm/s printing speed, 0.8 mm nozzle diameter, and 0.5 mm layer height. The treatment with borax showed good shape fidelity during 12 h storage. The treated construct also showed considerably increased mechanical properties and antioxidant activities in comparison with the untreated construct. A stable 3D construct suitable for a variety of applications could be produced using CMFs and guar gum-based ink.
采用纤维素微纤维(CMFs, 1% w/v)和瓜尔胶(1-7 g/100 mL CMFs)制备了一种适合三维打印的生物材料墨水,并研究了硼砂处理结构体的打印后稳定性和抗氧化功能。流变分析、傅里叶变换红外光谱、x射线衍射和扫描电子显微镜显示了这两种聚合物形成互穿复合水凝胶的适宜性,这将有利于印刷。制备的复合水凝胶具有良好的结构、形态、热性能和织构性能。在填充密度为50%、打印速度为10 mm/s、喷嘴直径为0.8 mm、层高为0.5 mm的条件下,打印出具有最佳表面性能和流变性能的5%瓜尔胶复合材料,尺寸误差最小。硼砂处理在12 h的贮藏过程中表现出良好的保真度。与未处理的结构相比,处理后的结构也显示出显著增加的机械性能和抗氧化活性。使用CMFs和瓜尔胶基油墨可以产生适合各种应用的稳定3D结构。
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引用次数: 0
3D bioprinting for auricular reconstruction: A review and future perspectives 生物3D打印耳廓重建的研究进展及展望
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-08-07 DOI: 10.36922/ijb.0898
Anna Onderková, Deepak M. Kalaskar
Congenital abnormalities or acquired trauma to the auricle can result in a need for ear reconstruction and negatively impact a person’s quality of life. Autografting, alloplastic implants, and prostheses are available to treat these issues, but each requires multiple surgical stages and has limitations and complications. Three-dimensional (3D) bioprinting promises to allow the creation of living, patient-specific ear substitutes that could reduce operative morbidity. In this review, we evaluate the current state of 3D bioprinting methods through a systematic search and review of 27 studies, aiming to examine this emerging technology within the context of existing reconstructive options. The included studies were all non-randomized experimental studies, except for a single pilot clinical trial. Most of these studies involved both in vitro and in vivo experiments demonstrating the potential of 3D bioprinting to create functional and anatomically accurate engineered cartilaginous frameworks for surgical implantation. Various ways of optimizing printing were identified, from choosing the most suitable material and cell type for the construct to addressing scaffold deformation and shrinkage issues. 3D printing has the potential to revolutionize reconstructive ear surgery by creating functional and aesthetically pleasing auricles. While more research into printing parameters, bioinks, cell types, and materials could optimize results, the next step is to conduct long-term in vivo clinical trials in humans.
先天性耳廓畸形或获得性耳廓创伤可导致耳廓重建的需要,并对一个人的生活质量产生负面影响。自体移植物、同种异体植入物和假体可用于治疗这些问题,但每一种都需要多个手术阶段,并且有局限性和并发症。三维(3D)生物打印技术有望创造出活的、针对患者的耳部替代品,从而降低手术的发病率。在这篇综述中,我们通过对27项研究的系统搜索和回顾来评估3D生物打印方法的现状,旨在在现有重建选择的背景下研究这一新兴技术。纳入的研究除一项临床试验外均为非随机实验研究。这些研究大多涉及体外和体内实验,证明了3D生物打印在为外科植入创造功能性和解剖学精确的工程软骨框架方面的潜力。从选择最合适的材料和细胞类型到解决支架变形和收缩问题,确定了各种优化打印的方法。3D打印有可能通过创造功能和美观的耳廓来彻底改变耳部重建手术。虽然对打印参数、生物墨水、细胞类型和材料的更多研究可以优化结果,但下一步是在人体中进行长期的体内临床试验。
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引用次数: 0
Near-infrared controlled release of mesenchymal stem cells secretome from bioprinted graphene-based microbeads for nerve regeneration 生物打印石墨烯基微珠近红外控制释放间充质干细胞分泌组用于神经再生
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-08-04 DOI: 10.36922/ijb.1045
G. Perini, V. Palmieri, M. D’Ascenzo, C. Colussi, C. Grassi, G. Friggeri, A. Augello, Li-ying Cui, M. Papi, M. De Spirito
 Nerve damage is a prevalent and debilitating condition with limited treatment options. Recent years have seen an increased incidence of neural damage due to factors such as aging populations and traumatic brain injuries. Addressing the urgent need for effective therapies, this study explores the controlled delivery of mesenchymal stem cells (MSCs) secretome, a complex mixture of bioactive factors, which is currently being investigated for its potential in nerve regeneration. The secretome offers significant advantages over stem cells themselves, as it can be more easily characterized and controlled, enabling precise regulation of therapeutic interventions. However, the challenge lies in delivering the secretome specifically to the target anatomical region. To overcome this limitation, we propose a novel approach utilizing near-infrared (NIR) radiation-responsive bioprinted alginate-graphene oxide (AGO) microbeads. Graphene oxide (GO) is a highly biocompatible material with unique properties, including NIR responsiveness, enabling controlled release of therapeutic agents upon NIR exposure. We hypothesized that AGO microbeads could encapsulate MSCs secretome and release it in a controlled manner using NIR radiation. To investigate our hypothesis, controlled damage was induced to hippocampal neurons, and MSCs secretome was encapsulated within AGO microbeads. Subsequently, NIR radiation was applied to trigger the release of the secretome. We compared the efficacy of MSCs secretome with that of astrocytes, which also possess nerve growth and proliferation-promoting capabilities. Our findings demonstrated that the controlled release of MSCs secretome from AGO microbeads through non-invasive NIR radiation significantly promoted the proliferation and regeneration of neurons following nerve injury. AGO microbeads offer multiple advantages over conventional delivery methods, including precise control over the timing, location, and dosage of therapeutic agents. Furthermore, the potential for reduced immunogenicity and tumorigenicity enhances the safety profile of the therapy. Consequently, this study presents a promising avenue for the development of MSC-based therapies for nerve regeneration, with implications for the treatment of various neuropathies and injuries. 
神经损伤是一种普遍且使人衰弱的疾病,治疗方法有限。近年来,由于人口老龄化和创伤性脑损伤等因素,神经损伤的发生率有所增加。为了解决有效治疗的迫切需要,本研究探索了间充质干细胞(MSCs)分泌组的控制递送,这是一种复杂的生物活性因子混合物,目前正在研究其在神经再生中的潜力。与干细胞本身相比,分泌组具有显著的优势,因为它可以更容易地表征和控制,从而能够精确地调节治疗干预。然而,挑战在于将分泌组特异性地递送到目标解剖区域。为了克服这一限制,我们提出了一种利用近红外(NIR)辐射响应生物打印海藻酸-氧化石墨烯(AGO)微珠的新方法。氧化石墨烯(GO)是一种高度生物相容性的材料,具有独特的性能,包括近红外响应性,可以在近红外照射下控制治疗剂的释放。我们假设AGO微珠可以包封MSCs分泌组,并通过近红外辐射以可控的方式释放。为了验证我们的假设,我们对海马神经元进行了控制性损伤,并将MSCs分泌组包裹在AGO微珠中。随后,应用近红外辐射触发分泌组的释放。我们比较了MSCs分泌组和星形胶质细胞的功效,星形胶质细胞也具有促进神经生长和增殖的能力。我们的研究结果表明,通过非侵入性近红外辐射,AGO微珠控制MSCs分泌组的释放显著促进了神经损伤后神经元的增殖和再生。AGO微珠与传统的给药方法相比具有多种优势,包括对治疗药物的时间、位置和剂量的精确控制。此外,降低免疫原性和致瘤性的潜力增强了该疗法的安全性。因此,本研究为基于msc的神经再生疗法的发展提供了一条有希望的途径,对各种神经病变和损伤的治疗具有重要意义。
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引用次数: 0
3D printing of costal cartilage models with fine fidelity and biomimetic mechanical performance for ear reconstruction simulation 3D打印具有良好保真度和仿生力学性能的肋软骨模型用于耳部重建模拟
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-08-03 DOI: 10.36922/ijb.1007
Senmao Wang, Di Wang, Liya Jia, Y. Yue, Genli Wu, Yuyun Chu, Qian Wang, Bo Pan, Haiyue Jiang, Lin Lin
 Patient-based training is difficult in ear reconstruction surgery; therefore, costal cartilage models are required for surgical education and pre-operative simulation. Here, we aimed to fabricate personalized models with mechanical and structural similarity to native costal cartilage to simulate ear reconstruction in microtia patients. To achieve this, the stiffness, hardness, and suture retention ability of both native costal cartilage and printed silicone were experimentally examined in vitro. Rheological tests and three-dimensional (3D) comparison methods were used to evaluate the printing ability and outcomes. The printed silicone models were used by residents to practice ear framework handcrafting during ear reconstruction surgery, and the residents’ learning curves were analyzed. In addition, the models were used for pre-operative simulation to study and optimize the surgical plan. The results showed that the consistency of mechanical properties within cartilage and silicone was verified. Printable silicone had good shear-thinning properties, and the printed structures had almost perfect printing fidelity. Residents who used printed silicone models enjoyed great progress and confidence after training. The pre-operative simulation optimized the carving scheme, reduced trauma in the operative site, and avoided wasting necessary cartilage tissue. Overall, fine-fidelity models created in this study were intended for surgical education and pre-operative simulation by applying 3D-printable (3DP) silicone, facilitating the optimization of surgical plans. Surgeons were satisfied with this kind of model and recognized the efficacy and great application value of 3D-printed silicone models for clinical practice.
耳部再造术中以患者为本的训练是困难的;因此,需要肋软骨模型进行手术教育和术前模拟。在这里,我们的目标是制造与天然肋软骨具有机械和结构相似性的个性化模型来模拟小耳症患者的耳部重建。为了实现这一目标,我们在体外实验检测了天然肋软骨和打印硅胶的刚度、硬度和缝线保持能力。采用流变学测试和三维(3D)对比方法来评估打印能力和效果。住院医师在耳廓重建手术中使用打印硅胶模型进行耳架手工制作练习,并分析住院医师的学习曲线。此外,利用模型进行术前模拟,研究和优化手术方案。结果表明,验证了软骨和硅胶内部力学性能的一致性。可打印硅胶具有良好的剪切减薄性能,打印结构具有近乎完美的打印保真度。使用硅胶打印模型的住院医师在培训后进步很大,信心也很足。术前模拟优化了雕刻方案,减少了手术部位的创伤,避免了必要软骨组织的浪费。总的来说,本研究中创建的精细保真模型旨在通过应用3d打印(3D-printable, 3DP)硅胶进行手术教育和术前模拟,促进手术计划的优化。外科医生对这种模型非常满意,也认可了3d打印硅胶模型在临床中的有效性和巨大的应用价值。
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引用次数: 0
3D bioprinting for vascular grafts and microvasculature 用于血管移植和微血管系统的3D生物打印
IF 8.4 3区 医学 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-08-02 DOI: 10.36922/ijb.0012
Junpeng Zhu, Xinwang Wang, Lin Lin, W. Zeng
Cardiovascular disease is the world’s leading cause of death, and there is a substantial clinical need for transplantable blood vessels. Through tissue vascular engineering technology, large blood vessel grafts with significant clinical effects have been synthesized. However, synthesizing vascular valves, small vessels up to 6 mm in diameter, and capillary networks up to 500 μm in diameter remains challenging due to the lack of precise manufacturing techniques. In particular, constructing a microvascular network in thick tissue is the technical bottleneck of organ transplantation. Three-dimensional (3D) bioprinting is a computer-assisted layer-by-layer deposition method that can deposit cells and biomaterials at a predetermined location, according to an accurate digital 3D model, to build a delicate and complex bionic structure. This review discusses the progress and limitations of 3D bioprinting in preparing large vessels and valves, small-diameter vessels, and microvascular networks. This paper focuses on improved printing technology and innovative bio-ink materials. The future application of 3D bioprinting is prospected in generating artificial blood vessel grafts and vascularized organs with full biological function.  
心血管疾病是世界上导致死亡的主要原因,对可移植血管的临床需求很大。通过组织血管工程技术,合成了具有显著临床效果的大血管移植物。然而,由于缺乏精确的制造技术,合成血管阀、直径达6mm的小血管和直径达500 μm的毛细血管网络仍然具有挑战性。特别是在厚组织中构建微血管网络是器官移植的技术瓶颈。三维(3D)生物打印是一种计算机辅助的逐层沉积方法,可以根据精确的数字3D模型将细胞和生物材料沉积在预定位置,以构建精致复杂的仿生结构。本文综述了3D生物打印在制备大血管和瓣膜、小直径血管和微血管网络方面的进展和局限性。本文的重点是改进的印刷技术和创新的生物墨水材料。展望了生物3D打印技术在制造人工血管移植物和具有完整生物功能的血管化器官方面的应用前景。
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引用次数: 1
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International Journal of Bioprinting
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