Pengbei Fan, Fanli Jin, Yanqin Qin, Yuanyuan Wu, Qingzhen Yang, Han Liu, Jiansheng Li
Lung tissue engineering (LTE) has gained significant attention as a highly promising and innovative strategy to tackle the formidable obstacles posed by lung-related diseases and the lack of compatible donor organs availability. In the realm of groundbreaking advancements in tissue engineering (TE), one particular technology that has emerged as a game-changer is three-dimensional (3D) bioprinting. It distinguishes itself by offering a potent and versatile approach to constructing intricate structures while opening up new horizons for TE and regenerative medicine (RM). This review focuses on the application of multiscale 3D bioprinting techniques in LTE and the reconstitution of lung tissue in vitro. We analyzed the key aspects such as bioink formulations and printing strategies utilized from macroscale 3D bioprinting to micro/nanoscale 3D bioprinting. Additionally, we evaluated the potential of multiscale bioprinting to replicate the complex architecture of the lung, ranging from macrostructures to micro/nanoscale features. We discussed the challenges and future directions in biofabrication approaches for LTE. Furthermore, we highlight the current progress and future perspectives in tissue reconstitution of lung in vitro, considering factors such as cell source, functionalization, and integration of physiological cues. Overall, multiscale 3D bioprinting offers exciting possibilities for the development of functional lung tissues, enabling disease modeling, new drug screening, and personalized regenerative therapies.
{"title":"Multiscale 3D bioprinting for the recapitulation of lung tissue","authors":"Pengbei Fan, Fanli Jin, Yanqin Qin, Yuanyuan Wu, Qingzhen Yang, Han Liu, Jiansheng Li","doi":"10.36922/ijb.1166","DOIUrl":"https://doi.org/10.36922/ijb.1166","url":null,"abstract":"Lung tissue engineering (LTE) has gained significant attention as a highly promising and innovative strategy to tackle the formidable obstacles posed by lung-related diseases and the lack of compatible donor organs availability. In the realm of groundbreaking advancements in tissue engineering (TE), one particular technology that has emerged as a game-changer is three-dimensional (3D) bioprinting. It distinguishes itself by offering a potent and versatile approach to constructing intricate structures while opening up new horizons for TE and regenerative medicine (RM). This review focuses on the application of multiscale 3D bioprinting techniques in LTE and the reconstitution of lung tissue in vitro. We analyzed the key aspects such as bioink formulations and printing strategies utilized from macroscale 3D bioprinting to micro/nanoscale 3D bioprinting. Additionally, we evaluated the potential of multiscale bioprinting to replicate the complex architecture of the lung, ranging from macrostructures to micro/nanoscale features. We discussed the challenges and future directions in biofabrication approaches for LTE. Furthermore, we highlight the current progress and future perspectives in tissue reconstitution of lung in vitro, considering factors such as cell source, functionalization, and integration of physiological cues. Overall, multiscale 3D bioprinting offers exciting possibilities for the development of functional lung tissues, enabling disease modeling, new drug screening, and personalized regenerative therapies.    ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135492003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linxuan Li, Dongdong Gu, He Liu, Han Zhang, Junhao Shan, Yijuan Zhang
The heat dissipation structure used in modern airborne radar chassis not only requires lightweight, but also pursues better mechanical properties and heat dissipation performance. In this study, a stochastically porous pomelo peel-inspired gradient structure was fabricated by laser powder bed fusion using Al-Mg-Sc-Zr powder. This study focused on the formability, microstructure, mechanical properties, and heat dissipation performance of the biomimetic structure through experimental and finite element analysis approaches. The influence of volume fraction (VF) on structural mechanical properties, deformation modes, stress distribution, and heat dissipation performance was investigated. The results showed that the mechanical properties of the structure declined as the VFs decreased. The optimal mechanical performance was obtained at the VF of 45%, where the compressive strength, specific energy absorption (Ws), and specific compressive strength values were measured to be 63.47 MPa, 34.84 J/g, and 142.16 MPa/(g·cm-3), respectively. Moreover, the Ws of the structures was higher than that of the reported aluminum alloy structures at the same VF. The biomimetic structure exhibited improved heat dissipation performance as the VFs decreased, with Reynolds number ranging from 2700 to 13,400. The structure of 30% VF with a remarkable heat transfer efficiency index of 1.86 displayed the best heat dissipation performance. In addition, compared with the traditional fin structures, the bionic structure possessed better thermal resistance, heat transfer efficiency index, and temperature uniformity at the same VF. This study demonstrated notable potential of pomelo peel-inspired design for lightweight load-bearing applications capable of heat-dissipating performance, providing a novel perspective for design and fabrication of versatile structures in the aviation field.
{"title":"Lightweight load-bearing heat dissipation multifunctional pomelo peel-inspired structures fabricated by laser powder bed fusion","authors":"Linxuan Li, Dongdong Gu, He Liu, Han Zhang, Junhao Shan, Yijuan Zhang","doi":"10.36922/ijb.1011","DOIUrl":"https://doi.org/10.36922/ijb.1011","url":null,"abstract":"   The heat dissipation structure used in modern airborne radar chassis not only requires lightweight, but also pursues better mechanical properties and heat dissipation performance. In this study, a stochastically porous pomelo peel-inspired gradient structure was fabricated by laser powder bed fusion using Al-Mg-Sc-Zr powder. This study focused on the formability, microstructure, mechanical properties, and heat dissipation performance of the biomimetic structure through experimental and finite element analysis approaches. The influence of volume fraction (VF) on structural mechanical properties, deformation modes, stress distribution, and heat dissipation performance was investigated. The results showed that the mechanical properties of the structure declined as the VFs decreased. The optimal mechanical performance was obtained at the VF of 45%, where the compressive strength, specific energy absorption (Ws), and specific compressive strength values were measured to be 63.47 MPa, 34.84 J/g, and 142.16 MPa/(g·cm-3), respectively. Moreover, the Ws of the structures was higher than that of the reported aluminum alloy structures at the same VF. The biomimetic structure exhibited improved heat dissipation performance as the VFs decreased, with Reynolds number ranging from 2700 to 13,400. The structure of 30% VF with a remarkable heat transfer efficiency index of 1.86 displayed the best heat dissipation performance. In addition, compared with the traditional fin structures, the bionic structure possessed better thermal resistance, heat transfer efficiency index, and temperature uniformity at the same VF. This study demonstrated notable potential of pomelo peel-inspired design for lightweight load-bearing applications capable of heat-dissipating performance, providing a novel perspective for design and fabrication of versatile structures in the aviation field.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136242101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Type 1 diabetes (T1D) is characterized by the degeneration of insulin-producing beta cells within pancreatic islets, resulting in impaired endogenous insulin synthesis, which necessitates exogenous insulin therapy. Although intensive insulin therapy has been effective in many patients, a subset of individuals with unstable T1D encounter challenges in maintaining optimal glycemic control through insulin injections. Pancreatic islet transplantation has emerged as a promising therapeutic alternative for such patients, offering enhanced glucose regulation, reduced risk of complications, and liberation from exogenous insulin reliance. However, impediments such as immune rejection and the need for an optimal transplantation environment limit the success of islet transplantation. Revascularization, a crucial requirement for proper islet functionality, poses a challenge in transplantation settings. Biomaterial-based biofabrication approaches have attracted considerable attention to address these challenges. Biomaterials engineered to emulate the native extracellular matrix provide a supportive environment for islet viability and functionality. This review article presents the recent advancements in biomaterials and biofabrication technologies aimed at engineering cell delivery systems to enhance the efficacy of islet transplantation. Immune protection and vascularization strategies are discussed, key biomaterials employed in islet transplantation are highlighted, and various biofabrication techniques, including electrospinning, microfabrication, and bioprinting, are explored. Furthermore, the future directions and challenges in the field of cell delivery systems for islet transplantation are discussed. The integration of appropriate biomaterials and biofabrication methods has significant potential to promote successful islet transplantation by facilitating vascularization and bolstering the immune defense mechanisms.
{"title":"Advancements in biomaterials and biofabrication for enhancing islet transplantation","authors":"Dayoon Kang, Jaewook Kim, Jinah Jang","doi":"10.36922/ijb.1024","DOIUrl":"https://doi.org/10.36922/ijb.1024","url":null,"abstract":"Type 1 diabetes (T1D) is characterized by the degeneration of insulin-producing beta cells within pancreatic islets, resulting in impaired endogenous insulin synthesis, which necessitates exogenous insulin therapy. Although intensive insulin therapy has been effective in many patients, a subset of individuals with unstable T1D encounter challenges in maintaining optimal glycemic control through insulin injections. Pancreatic islet transplantation has emerged as a promising therapeutic alternative for such patients, offering enhanced glucose regulation, reduced risk of complications, and liberation from exogenous insulin reliance. However, impediments such as immune rejection and the need for an optimal transplantation environment limit the success of islet transplantation. Revascularization, a crucial requirement for proper islet functionality, poses a challenge in transplantation settings. Biomaterial-based biofabrication approaches have attracted considerable attention to address these challenges. Biomaterials engineered to emulate the native extracellular matrix provide a supportive environment for islet viability and functionality. This review article presents the recent advancements in biomaterials and biofabrication technologies aimed at engineering cell delivery systems to enhance the efficacy of islet transplantation. Immune protection and vascularization strategies are discussed, key biomaterials employed in islet transplantation are highlighted, and various biofabrication techniques, including electrospinning, microfabrication, and bioprinting, are explored. Furthermore, the future directions and challenges in the field of cell delivery systems for islet transplantation are discussed. The integration of appropriate biomaterials and biofabrication methods has significant potential to promote successful islet transplantation by facilitating vascularization and bolstering the immune defense mechanisms.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135236027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the increasing incidence and mortality rates, cancer remains a major health challenge in the world. Despite advances in therapies and clinical programs, the efficacy of anti-cancer drugs often fails to translate from pre-clinical models to patient clinical trials. To date, pre-clinical cancer models, including two-dimensional cell cultures and animal models, have limited versatility and accuracy in recapitulating the complexity of human cancer. To address these limitations, a growing focus has fostered the development of three-dimensional (3D) tumor models that closely resemble the in vivo tumor microenvironment and heterogeneity. Recent efforts have leveraged bioengineering technologies, such as biofabrication, to engineer new platforms that mimic healthy and diseased organs, aiming to overcome the shortcomings of conventional models, such as for musculoskeletal tissues. Notably, 3D bioprinting has emerged as a powerful tool in cancer research, offering precise control over cell and biomaterial deposition to fabricate architecturally complex and reproducible functional models. The following review underscores the urgent need for more accurate and relevant 3D tumor models, highlighting the advantages of the use of biofabrication approaches to engineer new biomimetics platforms. We provide an updated discussion on the role of bioengineering technologies in cancer research and modeling with particular focus on 3D bioprinting platforms, as well as a close view on biomaterial inks and 3D bioprinting technologies employed in cancer modeling. Further insights into the 3D bioprinting tissue-specific modeling panorama are presented in this paper, offering a comprehensive overview of the new possibilities for cancer study and drug discovery.
{"title":"Biomimetic 3D bioprinting approaches to engineer the tumor microenvironment","authors":"Fabiano Bini, Salvatore D’Alessandro, Tarun Agarwal, Daniele Marciano, Serena Duchi, Enrico Lucarelli, Giancarlo Ruocco, Franco Marinozzi, Gianluca Cidonio","doi":"10.36922/ijb.1022","DOIUrl":"https://doi.org/10.36922/ijb.1022","url":null,"abstract":"With the increasing incidence and mortality rates, cancer remains a major health challenge in the world. Despite advances in therapies and clinical programs, the efficacy of anti-cancer drugs often fails to translate from pre-clinical models to patient clinical trials. To date, pre-clinical cancer models, including two-dimensional cell cultures and animal models, have limited versatility and accuracy in recapitulating the complexity of human cancer. To address these limitations, a growing focus has fostered the development of three-dimensional (3D) tumor models that closely resemble the in vivo tumor microenvironment and heterogeneity. Recent efforts have leveraged bioengineering technologies, such as biofabrication, to engineer new platforms that mimic healthy and diseased organs, aiming to overcome the shortcomings of conventional models, such as for musculoskeletal tissues. Notably, 3D bioprinting has emerged as a powerful tool in cancer research, offering precise control over cell and biomaterial deposition to fabricate architecturally complex and reproducible functional models. The following review underscores the urgent need for more accurate and relevant 3D tumor models, highlighting the advantages of the use of biofabrication approaches to engineer new biomimetics platforms. We provide an updated discussion on the role of bioengineering technologies in cancer research and modeling with particular focus on 3D bioprinting platforms, as well as a close view on biomaterial inks and 3D bioprinting technologies employed in cancer modeling. Further insights into the 3D bioprinting tissue-specific modeling panorama are presented in this paper, offering a comprehensive overview of the new possibilities for cancer study and drug discovery.  ","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135717934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aflatoxin B1, found in a variety of foods, is a mycotoxin known to cause cancer. Therefore, humans may be exposed to it through their daily diet. In this study, a three-dimensional (3D) tumor spheroid model was developed via 3D bioprinting to examine whether exposure of HepG2 liver tumor spheroids to aflatoxin B1 can increase the population of drug-resistant liver cancer cells in a single tumor spheroid. Two biomarkers, CD133 (prominin-1) and aldehyde dehydrogenase 1 (ALDH1), were used to identify drug-resistant cancer cells formed in the single liver tumor spheroids. The induction of drug-resistant cancer cells in the single tumor spheroids was examined through single spheroid imaging and fluorescence-activated cell sorting (FACS). The increase of drug-resistant cancer cells, which was caused by aflatoxin B1 in a dose-dependent manner, was quantitatively monitored at the single tumor spheroid level using both methods. 3D bioprinting-fabricated single liver tumor spheroid model successfully determined drug-resistant liver cancer cells caused by aflatoxin B1
{"title":"3D bioprinting-based single liver tumor spheroid analysis for aflatoxin B1-induced drug-resistant cancer cell","authors":"Viet Phuong Cao, Sera Hong, Joon Myong Song","doi":"10.36922/ijb.0985","DOIUrl":"https://doi.org/10.36922/ijb.0985","url":null,"abstract":"Aflatoxin B1, found in a variety of foods, is a mycotoxin known to cause cancer. Therefore, humans may be exposed to it through their daily diet. In this study, a three-dimensional (3D) tumor spheroid model was developed via 3D bioprinting to examine whether exposure of HepG2 liver tumor spheroids to aflatoxin B1 can increase the population of drug-resistant liver cancer cells in a single tumor spheroid. Two biomarkers, CD133 (prominin-1) and aldehyde dehydrogenase 1 (ALDH1), were used to identify drug-resistant cancer cells formed in the single liver tumor spheroids. The induction of drug-resistant cancer cells in the single tumor spheroids was examined through single spheroid imaging and fluorescence-activated cell sorting (FACS). The increase of drug-resistant cancer cells, which was caused by aflatoxin B1 in a dose-dependent manner, was quantitatively monitored at the single tumor spheroid level using both methods. 3D bioprinting-fabricated single liver tumor spheroid model successfully determined drug-resistant liver cancer cells caused by aflatoxin B1","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136214709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yubo Shi, Lei Wang, Liguo Sun, Zhennan Qiu, Xiaoli Qu, Jingyi Dang, Zhao Zhang, Jiankang He, Hongbin Fan
Melt electrospinning writing (MEW) is a promising three-dimensional (3D) printing technology that enables the creation of scaffolds with highly ordered microfibers. Polycaprolactone (PCL) is an ideal material for MEW scaffold fabrication due to its exceptional printability. However, its low cellular affinity can hinder its performance in bone tissue engineering. This study aimed to explore the potential of NaOH treatment as a means of enhancing the cytocompatibility and osteoinductive properties of PCL scaffolds. After modification with a NaOH solution, the physiochemical properties of the MEW PCL scaffold were analyzed. The surface of the scaffold was found to have nanopits and nanogrooves, which differed from the smooth surface of the PCL scaffold. Atomic force microscopy and automatic water contact angle assays revealed an increase in surface roughness and wettability, both of which were found to be beneficial for cell proliferation and adhesion. In vitro experiments demonstrated that the NaOH-treated surface was able to induce osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) via the integrinα2/β1-PI3K-Akt signaling pathway, which had not been previously observed. The study involved implanting PCL scaffold to repair a cranial defect. After 1 and 3 months of implantation, histological analysis and micro-computed tomography scans showed a higher amount of newly formed bone on the NaOH-treated PCL scaffolds compared to the PCL scaffold. The study concluded that NaOH treatment was a simple and effective way to enhance cellular affinity and osteoinductive property of MEW PCL scaffold. This strategy may provide a cost-efficient method for promoting bone regeneration.
{"title":"Melt electrospinning writing PCL scaffolds after alkaline modification with outstanding cytocompatibility and osteoinduction","authors":"Yubo Shi, Lei Wang, Liguo Sun, Zhennan Qiu, Xiaoli Qu, Jingyi Dang, Zhao Zhang, Jiankang He, Hongbin Fan","doi":"10.36922/ijb.1071","DOIUrl":"https://doi.org/10.36922/ijb.1071","url":null,"abstract":"Melt electrospinning writing (MEW) is a promising three-dimensional (3D) printing technology that enables the creation of scaffolds with highly ordered microfibers. Polycaprolactone (PCL) is an ideal material for MEW scaffold fabrication due to its exceptional printability. However, its low cellular affinity can hinder its performance in bone tissue engineering. This study aimed to explore the potential of NaOH treatment as a means of enhancing the cytocompatibility and osteoinductive properties of PCL scaffolds. After modification with a NaOH solution, the physiochemical properties of the MEW PCL scaffold were analyzed. The surface of the scaffold was found to have nanopits and nanogrooves, which differed from the smooth surface of the PCL scaffold. Atomic force microscopy and automatic water contact angle assays revealed an increase in surface roughness and wettability, both of which were found to be beneficial for cell proliferation and adhesion. In vitro experiments demonstrated that the NaOH-treated surface was able to induce osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) via the integrinα2/β1-PI3K-Akt signaling pathway, which had not been previously observed. The study involved implanting PCL scaffold to repair a cranial defect. After 1 and 3 months of implantation, histological analysis and micro-computed tomography scans showed a higher amount of newly formed bone on the NaOH-treated PCL scaffolds compared to the PCL scaffold. The study concluded that NaOH treatment was a simple and effective way to enhance cellular affinity and osteoinductive property of MEW PCL scaffold. This strategy may provide a cost-efficient method for promoting bone regeneration.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"7 4","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72426440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kun Liu, Nan Hu, Zhihai Yu, Xin-Zheng Zhang, Hualin Ma, Huawei Qu, Changshun Ruan
Three-dimensional (3D) printing with highly flexible fabrication offers unlimited possibilities to create complex constructs. With the addition of active substances such as biomaterials, living cells, and growth factors, 3D printing can be upgraded to 3D bioprinting, endowing fabricated constructs with biological functions. Urology, as one of the important branches of clinical medicine, covers a variety of organs in the human body, such as kidneys, bladder, urethra, and prostate. The urological organs are multi-tubular, heterogeneous, and anisotropic, bringing huge challenges to 3D printing and bioprinting. This review aims to summarize the development of 3D printing and bioprinting technologies in urology in the last decade based on the Science Citation Index-Expanded (SCI-E) in the Web of Science Core Collection online database (Clarivate). First, we demonstrate the search strategies for published papers using the keywords such as “3D printing,” “3D bioprinting,” and “urology.” Then, eight common 3D printing technologies were introduced in detail with their characteristics, advantages, and disadvantages. Furthermore, the application of 3D printing in urology was explored, such as the fabrication of diseased organs for doctor–patient communication, surgical planning, clinical teaching, and the creation of customized medical devices. Finally, we discuss the exploration of 3D bioprinting to create in vitro bionic 3D environment models for urology. Overall, 3D printing provides the technical support for urology to better serve patients and aid teaching, and 3D bioprinting enables the clinical applications of fabricated constructs for the replacement and repair of urologically damaged organs in future.
具有高度柔性制造的三维(3D)打印为创建复杂结构提供了无限的可能性。随着生物材料、活细胞、生长因子等活性物质的加入，3D打印可以升级为3D生物打印，使制造的结构物具有生物功能。泌尿外科是临床医学的重要分支之一，涵盖了人体的肾脏、膀胱、尿道、前列腺等多种器官。泌尿系统器官具有多管性、异质性和各向异性，这给3D打印和生物打印带来了巨大的挑战。本文以Web of Science Core Collection在线数据库(Clarivate)中的SCI-E为基础，综述了近十年来3D打印和生物打印技术在泌尿外科领域的发展。首先，我们演示了使用“3D打印”、“3D生物打印”和“泌尿学”等关键词搜索已发表论文的策略。然后，详细介绍了八种常见的3D打印技术，以及它们的特点、优缺点。此外，还探讨了3D打印在泌尿外科的应用，如用于医患交流、手术计划、临床教学的病变器官的制造以及定制医疗设备的创建。最后，我们讨论了3D生物打印在泌尿外科体外仿生3D环境模型中的探索。总体而言，3D打印为泌尿外科更好地服务患者和辅助教学提供了技术支持，3D生物打印为未来泌尿系统损伤器官的替换和修复提供了临床应用。
{"title":"3D printing and bioprinting in urology","authors":"Kun Liu, Nan Hu, Zhihai Yu, Xin-Zheng Zhang, Hualin Ma, Huawei Qu, Changshun Ruan","doi":"10.36922/ijb.0969","DOIUrl":"https://doi.org/10.36922/ijb.0969","url":null,"abstract":"Three-dimensional (3D) printing with highly flexible fabrication offers unlimited possibilities to create complex constructs. With the addition of active substances such as biomaterials, living cells, and growth factors, 3D printing can be upgraded to 3D bioprinting, endowing fabricated constructs with biological functions. Urology, as one of the important branches of clinical medicine, covers a variety of organs in the human body, such as kidneys, bladder, urethra, and prostate. The urological organs are multi-tubular, heterogeneous, and anisotropic, bringing huge challenges to 3D printing and bioprinting. This review aims to summarize the development of 3D printing and bioprinting technologies in urology in the last decade based on the Science Citation Index-Expanded (SCI-E) in the Web of Science Core Collection online database (Clarivate). First, we demonstrate the search strategies for published papers using the keywords such as “3D printing,” “3D bioprinting,” and “urology.” Then, eight common 3D printing technologies were introduced in detail with their characteristics, advantages, and disadvantages. Furthermore, the application of 3D printing in urology was explored, such as the fabrication of diseased organs for doctor–patient communication, surgical planning, clinical teaching, and the creation of customized medical devices. Finally, we discuss the exploration of 3D bioprinting to create in vitro bionic 3D environment models for urology. Overall, 3D printing provides the technical support for urology to better serve patients and aid teaching, and 3D bioprinting enables the clinical applications of fabricated constructs for the replacement and repair of urologically damaged organs in future.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"24 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79904793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dageon Oh, M. Shirzad, Min Chang Kim, Eun-Jae Chung, S. Y. Nam
In this study, a rheology-informed hierarchical machine learning (RIHML) model was developed to improve the prediction accuracy of the printing resolution of constructs fabricated by extrusion-based bioprinting. Specifically, the RIHML model, as well as conventional models such as the concentration-dependent model and printing parameter-dependent model, was trained and tested using a small dataset of bioink properties and printing parameters. Interestingly, the results showed that the RIHML model exhibited the lowest error percentage in predicting the printing resolution for different printing parameters such as nozzle velocities and pressures, as well as for different concentrations of the bioink constituents. Besides, the RIHML model could predict the printing resolution with reasonably low errors even when using a new material added to the alginate-based bioink, which is a challenging task for conventional models. Overall, the results indicate that the RIHML model can be a useful tool to predict the printing resolution of extrusion-based bioprinting, and it is versatile and expandable compared to conventional models since the RIHML model can easily generalize and embrace new data.
{"title":"Rheology-informed hierarchical machine learning model for the prediction of printing resolution in extrusion-based bioprinting","authors":"Dageon Oh, M. Shirzad, Min Chang Kim, Eun-Jae Chung, S. Y. Nam","doi":"10.36922/ijb.1280","DOIUrl":"https://doi.org/10.36922/ijb.1280","url":null,"abstract":"In this study, a rheology-informed hierarchical machine learning (RIHML) model was developed to improve the prediction accuracy of the printing resolution of constructs fabricated by extrusion-based bioprinting. Specifically, the RIHML model, as well as conventional models such as the concentration-dependent model and printing parameter-dependent model, was trained and tested using a small dataset of bioink properties and printing parameters. Interestingly, the results showed that the RIHML model exhibited the lowest error percentage in predicting the printing resolution for different printing parameters such as nozzle velocities and pressures, as well as for different concentrations of the bioink constituents. Besides, the RIHML model could predict the printing resolution with reasonably low errors even when using a new material added to the alginate-based bioink, which is a challenging task for conventional models. Overall, the results indicate that the RIHML model can be a useful tool to predict the printing resolution of extrusion-based bioprinting, and it is versatile and expandable compared to conventional models since the RIHML model can easily generalize and embrace new data.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"2013 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86301473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Olajide Emmanuel Adedeji, Ju Hyun Min, Gi Eon Park, Hye Jee Kang, Ji-Young Choi, Mariam Omowunmi Aminu, Ocheme Boniface Ocheme, S. Joo, K. Moon, Young Hoon Jung
A biomaterial ink suitable for three-dimensional (3D) printing was developed using cellulose microfibrils (CMFs, 1% w/v) and guar gum (1–7 g/100 mL CMFs), and the post-printing stability and antioxidant functionality of the borax-treated construct were investigated. Rheological analysis, Fourier transform infrared spectrometry, X-ray diffractometry, and scanning electron microscopy revealed the suitability of the two polymers to form an interpenetrating composite hydrogel that would facilitate printability. The produced composite hydrogel showed good structural, morphological, thermal, and textural properties. CMFs with 5% guar gum showing optimal surface properties and rheological properties were printed with the least dimensional errors at 50% infill density, 10 mm/s printing speed, 0.8 mm nozzle diameter, and 0.5 mm layer height. The treatment with borax showed good shape fidelity during 12 h storage. The treated construct also showed considerably increased mechanical properties and antioxidant activities in comparison with the untreated construct. A stable 3D construct suitable for a variety of applications could be produced using CMFs and guar gum-based ink.
采用纤维素微纤维(CMFs, 1% w/v)和瓜尔胶(1-7 g/100 mL CMFs)制备了一种适合三维打印的生物材料墨水，并研究了硼砂处理结构体的打印后稳定性和抗氧化功能。流变分析、傅里叶变换红外光谱、x射线衍射和扫描电子显微镜显示了这两种聚合物形成互穿复合水凝胶的适宜性，这将有利于印刷。制备的复合水凝胶具有良好的结构、形态、热性能和织构性能。在填充密度为50%、打印速度为10 mm/s、喷嘴直径为0.8 mm、层高为0.5 mm的条件下，打印出具有最佳表面性能和流变性能的5%瓜尔胶复合材料，尺寸误差最小。硼砂处理在12 h的贮藏过程中表现出良好的保真度。与未处理的结构相比，处理后的结构也显示出显著增加的机械性能和抗氧化活性。使用CMFs和瓜尔胶基油墨可以产生适合各种应用的稳定3D结构。
{"title":"Development of a 3D-printable matrix using cellulose microfibrils/guar gum-based hydrogels and its post-printing antioxidant activity ","authors":"Olajide Emmanuel Adedeji, Ju Hyun Min, Gi Eon Park, Hye Jee Kang, Ji-Young Choi, Mariam Omowunmi Aminu, Ocheme Boniface Ocheme, S. Joo, K. Moon, Young Hoon Jung","doi":"10.36922/ijb.0164","DOIUrl":"https://doi.org/10.36922/ijb.0164","url":null,"abstract":"A biomaterial ink suitable for three-dimensional (3D) printing was developed using cellulose microfibrils (CMFs, 1% w/v) and guar gum (1–7 g/100 mL CMFs), and the post-printing stability and antioxidant functionality of the borax-treated construct were investigated. Rheological analysis, Fourier transform infrared spectrometry, X-ray diffractometry, and scanning electron microscopy revealed the suitability of the two polymers to form an interpenetrating composite hydrogel that would facilitate printability. The produced composite hydrogel showed good structural, morphological, thermal, and textural properties. CMFs with 5% guar gum showing optimal surface properties and rheological properties were printed with the least dimensional errors at 50% infill density, 10 mm/s printing speed, 0.8 mm nozzle diameter, and 0.5 mm layer height. The treatment with borax showed good shape fidelity during 12 h storage. The treated construct also showed considerably increased mechanical properties and antioxidant activities in comparison with the untreated construct. A stable 3D construct suitable for a variety of applications could be produced using CMFs and guar gum-based ink.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"25 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81431369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Congenital abnormalities or acquired trauma to the auricle can result in a need for ear reconstruction and negatively impact a person’s quality of life. Autografting, alloplastic implants, and prostheses are available to treat these issues, but each requires multiple surgical stages and has limitations and complications. Three-dimensional (3D) bioprinting promises to allow the creation of living, patient-specific ear substitutes that could reduce operative morbidity. In this review, we evaluate the current state of 3D bioprinting methods through a systematic search and review of 27 studies, aiming to examine this emerging technology within the context of existing reconstructive options. The included studies were all non-randomized experimental studies, except for a single pilot clinical trial. Most of these studies involved both in vitro and in vivo experiments demonstrating the potential of 3D bioprinting to create functional and anatomically accurate engineered cartilaginous frameworks for surgical implantation. Various ways of optimizing printing were identified, from choosing the most suitable material and cell type for the construct to addressing scaffold deformation and shrinkage issues. 3D printing has the potential to revolutionize reconstructive ear surgery by creating functional and aesthetically pleasing auricles. While more research into printing parameters, bioinks, cell types, and materials could optimize results, the next step is to conduct long-term in vivo clinical trials in humans.
{"title":"3D bioprinting for auricular reconstruction: A review and future perspectives","authors":"Anna Onderková, Deepak M. Kalaskar","doi":"10.36922/ijb.0898","DOIUrl":"https://doi.org/10.36922/ijb.0898","url":null,"abstract":"Congenital abnormalities or acquired trauma to the auricle can result in a need for ear reconstruction and negatively impact a person’s quality of life. Autografting, alloplastic implants, and prostheses are available to treat these issues, but each requires multiple surgical stages and has limitations and complications. Three-dimensional (3D) bioprinting promises to allow the creation of living, patient-specific ear substitutes that could reduce operative morbidity. In this review, we evaluate the current state of 3D bioprinting methods through a systematic search and review of 27 studies, aiming to examine this emerging technology within the context of existing reconstructive options. The included studies were all non-randomized experimental studies, except for a single pilot clinical trial. Most of these studies involved both in vitro and in vivo experiments demonstrating the potential of 3D bioprinting to create functional and anatomically accurate engineered cartilaginous frameworks for surgical implantation. Various ways of optimizing printing were identified, from choosing the most suitable material and cell type for the construct to addressing scaffold deformation and shrinkage issues. 3D printing has the potential to revolutionize reconstructive ear surgery by creating functional and aesthetically pleasing auricles. While more research into printing parameters, bioinks, cell types, and materials could optimize results, the next step is to conduct long-term in vivo clinical trials in humans.","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":"18 1","pages":""},"PeriodicalIF":8.4,"publicationDate":"2023-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81734475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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