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Comparison of the blood gas analyser Edan i15 Vet with the blood gas analyser Gem Premier 3000 for the analysis of blood gases, electrolytes, hemoglobin and hematocrit in lactating Holstein 血气分析仪Edan i15 Vet与血气分析仪Gem Premier 3000的比较,用于分析哺乳期荷斯坦犬的血气、电解质、血红蛋白和红细胞压积
IF 0.4 4区 农林科学 Q3 Veterinary Pub Date : 2023-01-01 DOI: 10.21521/mw.6728
M. Metin, K. Aksoy, Aytaç Pekmezci, A. Deniz
A point-of-care blood gas analyser, Edan i15 Vet (EDAN), was compared with a benchtop blood gas analyser, GEM Premier 3000 (GEM). GEM and EDAN were used to analyse whole blood from 123 lactating Holsteins within one month of calving for blood gases, electrolytes, hematocrit and hemoglobin. EDAN and GEM showed significant linear correlations for blood gases, electrolytes, Hct and Hb. The 95% confidence intervals (CI) of intercept in Passing-Bablok regressions included zero in pCO2, bicarbonate, TCO2, BE ecf, BE B, hematocrit, and pH, but not for Na+, K+, pO2 and sO2. The CI of the slope included 1.0 for Na+, K+, pCO2, bicarbonate, TCO2, BE ecf, BE B, hematocrit, and pH, but not for pO2, sO2, and hemoglobin. The Bland-Altman plots between EDAN and GEM showed a bias of 1.4% for Na+, 2.4% for K+, –1.6% for pCO2, 3.0% for pO2, –5.3% for bicarbonate, 2.8% for SO2, –7.3% for TCO2, 10.4% for Hct, 21.2% for Hb, –25.1% for BE B and –38.5% for BE ecf. The biases in the analysis of certain estimated parameters were much higher (> 5%) than for measured parameters except for Hct. Parity did not correlate with blood gas parameters but blood pH correlated negatively with K+, pCO2 and positively with pO2, TCO2, sO2, bicarbonate, BE ecf and BE B. The postpartum time correlated positively with pCO2, TCO2, BE and bicarbonate and negatively with Hct and Hb values. Reference values (2.5-97.5% quartiles) were determined for each parameter. Conclusively, EDAN can be used interchangeably with GEM for the analysis of blood pH, K+, pCO2 and HCO3 – as they show acceptable moderate agreement. However, they did not agree for other parameters such as TCO2, BE ecf, BE B, Hb, Hct, sO2, Na+, and pO2, therefore, reference values for each parameter were set for GEM and EDAN.
将现场血气分析仪Edan i15 Vet (Edan)与台式血气分析仪GEM Premier 3000 (GEM)进行比较。采用GEM和EDAN对123头泌乳期荷斯坦奶牛1个月内的全血进行血气、电解质、红细胞压积和血红蛋白分析。EDAN和GEM与血气、电解质、Hct和Hb呈显著的线性相关。passin - bablok回归的95%置信区间(CI)包括pCO2、碳酸氢盐、TCO2、BE ecf、BE B、红细胞压积和pH为零,但Na+、K+、pO2和sO2不为零。Na+、K+、pCO2、碳酸氢盐、TCO2、BE ecf、BE B、红细胞压积和pH的斜率CI为1.0,但pO2、sO2和血红蛋白的斜率CI不为1.0。EDAN和GEM之间的Bland-Altman图显示Na+的偏差为1.4%,K+的偏差为2.4%,pCO2的偏差为-1.6%,pO2的偏差为3.0%,碳酸氢盐的偏差为-5.3%,SO2的偏差为2.8%,TCO2的偏差为-7.3%,Hct的偏差为10.4%,Hb的偏差为21.2%,BE B的偏差为-25.1%,BE ecf的偏差为-38.5%。除Hct外,某些估计参数的分析偏差比测量参数高得多(约5%)。胎次与血气参数无相关性,而血pH值与K+、pCO2呈负相关,与pO2、TCO2、sO2、碳酸氢盐、BE、ecf、BE b呈正相关。产后时间与pCO2、TCO2、BE、碳酸氢盐呈正相关,与Hct、Hb值呈负相关。为每个参数确定参考值(2.5-97.5%四分位数)。最后,EDAN可以与GEM互换用于血液pH、K+、pCO2和HCO3 -的分析,因为它们显示出可接受的适度一致性。但对于TCO2、BE ecf、BE B、Hb、Hct、sO2、Na+、pO2等参数,双方意见不一致,因此对GEM和EDAN设置了各参数的参考值。
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引用次数: 0
Molecular background of the merle coat color in dogs and its health consequences 犬山鸟色被毛的分子背景及其对健康的影响
IF 0.4 4区 农林科学 Q3 Veterinary Pub Date : 2023-01-01 DOI: 10.21521/mw.6769
I. Szatkowska, Martyna Otręba, J. Korbecki, E. Czerniawska-Piątkowska, J. Wójcik, W. Grzesiak, D. Zaborski
Coat colour in dogs is influenced by many genes and their polymorphic forms. The main coat colour pattern depends on pigment type, i.e. eumelanin or pheomelanin, whose synthesis is regulated by tyrosinase encoded by the TYRP1 gene. However, other genes also contribute to the ultimate phenotype. One of them is PMEL responsible for the merle coat colour. This review focuses on the role of melanocytes in the phenotypic expression of coat colour, the merle phenotype and genotype, phenotypic diversity of the M allele carriers, health consequences for the carriers of the allele responsible for the merle coat colour and problems with the identification of the M allele carriers based on phenotype and dog breeds with the merle coat colour accepted by the FCI.
狗的毛色受许多基因及其多态性的影响。主要的皮毛颜色模式取决于色素类型,即真黑素或褐黑素,其合成受TYRP1基因编码的酪氨酸酶调节。然而,其他基因也有助于最终的表型。其中之一是PMEL负责山鸟色毛色。这篇综述的重点是黑素细胞在毛色表型表达中的作用,山鸟色表型和基因型,M等位基因携带者的表型多样性,负责山鸟色毛色的等位基因携带者的健康后果,以及基于表型识别M等位基因携带者的问题和FCI接受的山鸟色毛色犬种。
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引用次数: 0
Effect of Selected Factors on Features of Short-Term Social Isolation of Horses: A Pilot Study 选择因素对马短期社会隔离特征的影响:一项初步研究
IF 0.4 4区 农林科学 Q3 Veterinary Pub Date : 2023-01-01 DOI: 10.21521/mw.6744
E. Jastrzębska, A. Wiśniewska, I. Wilk, T. Próchniak, I. Janczarek
This study analysed the effect of the sex of the horses as well as the type of their maintenance and use at the centre on the behavioural and physiological expression of short-term social isolation, which was assessed on the basis of heart and respiratory rates. A total of 27 clinically healthy horses were examined. The horses had social contact of various durations, were used with different regularity and were not equally accustomed to isolation. An isolation test was conducted. The heart and respiratory rates were measured at rest and after isolation, and the differences between the values were calculated. Moreover, points were assigned for the effect of isolation. The behaviour in subsequent stages of isolation was also observed. It was found that the sex of the horses, the period spent daily in the paddock in the herd, the regularity of use and habituation to staying in isolation can all be classified as factors that have no effect on the heart rate, respiratory rate or behaviour during short-term isolation. It was suggested that the resting respiratory rate and the effect of isolation could be classified as features that are potentially useful in assessing horses’ adaptability to short-term social isolation.
这项研究分析了马的性别以及它们在中心的维护和使用类型对短期社会隔离的行为和生理表现的影响,这是根据心跳和呼吸速率进行评估的。对27匹临床健康马进行了检查。这些马的社会接触持续时间各不相同,使用的规律也各不相同,对隔离的习惯也不尽相同。进行了隔离试验。分别测量静息和隔离后的心率和呼吸频率,并计算两者之间的差异。此外,对隔离效果进行了打分。还观察了随后隔离阶段的行为。研究发现,马的性别、每天在马群围场中度过的时间、使用的规律性和对隔离的习惯都可以归类为在短期隔离期间对心率、呼吸频率或行为没有影响的因素。研究表明,静息呼吸率和隔离的影响可以被归类为评估马对短期社会隔离的适应性的潜在有用特征。
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引用次数: 0
Progress in vaccines against peste des petits ruminants virus 小反刍兽疫病毒疫苗研究进展
IF 0.4 4区 农林科学 Q3 Veterinary Pub Date : 2023-01-01 DOI: 10.21521/mw.6747
W. Niedbalski
Peste des petits ruminants (PPR) is a highly contagious and economically important viral disease of both domestic (goats and sheep) and wild small ruminants. Due to the devastating effect of this disease on livestock and livelihoods, the Food and Agriculture Organization of the United Nations (FAO) and the World Organization for Animal Health (WOAH) endorsed the Global Strategy for the Control and Eradication of PPR (PPR GCES) and launched the PPR Global Eradication Programme (PPR GEP) to eradicate PPRV by 2030. In order to achieve this goal, a potent, safe and efficacious live-attenuated PPR vaccine with long-lasting immunity is available for immunoprophylaxis. However, the live-attenuated PPR vaccines are thermolabile and require maintenance of an effective cold chain to deliver to the field. In addition, infected animals cannot be differentiated from vaccinated ones (DIVA). To overcome these limitations, some new generation PPR vaccines have been developed: poxvirus vaccine, positive and negative marker vaccine through reverse genetic approach, chimeric vaccine, anti-idiotypic vaccine, subunit vaccine, virus-like particles vaccine, edible vaccine and combined vaccines. Novel recombinant PPR DIVA vaccines were evaluated in goats for safety and efficacy, and all vaccinated animals were clinically protected against an intranasal PPRV challenge. Furthermore, newly developed ELISAs were capable of differentiating between infected and vaccinated animals. Therefore, these DIVA vaccines and the associated tests can facilitate the serological monitoring process and speed up global PPR eradication through vaccination.
小反刍兽疫(PPR)是一种在家养(山羊和绵羊)和野生小反刍动物中具有高度传染性和重要经济意义的病毒性疾病。鉴于这种疾病对牲畜和生计的破坏性影响,联合国粮食及农业组织(粮农组织)和世界动物卫生组织(世卫组织)批准了《控制和根除小反刍兽疫全球战略》(PPR GCES),并启动了小反刍兽疫全球根除计划(PPR GEP),目标是到2030年根除小反刍兽疫。为了实现这一目标,一种强效、安全、有效、具有持久免疫力的小反胃病毒减毒活疫苗可用于免疫预防。然而,小反刍猪瘟减毒活疫苗是耐热性的,需要维持有效的冷链才能送到现场。此外,无法将感染动物与接种疫苗的动物区分开来(DIVA)。为了克服这些局限性,一些新一代小反刍兽疫疫苗已经被开发出来:痘病毒疫苗、通过反向遗传的阳性和阴性标记疫苗、嵌合疫苗、抗独特型疫苗、亚单位疫苗、病毒样颗粒疫苗、可食用疫苗和联合疫苗。在山羊身上评估了新型重组小猪瘟DIVA疫苗的安全性和有效性,所有接种疫苗的动物在临床上都能抵抗鼻内小猪瘟的攻击。此外,新开发的elisa能够区分感染动物和接种动物。因此,这些DIVA疫苗和相关检测可以促进血清学监测过程,并通过疫苗接种加速全球根除小反胃病毒。
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引用次数: 0
Relation between defects in the lumbar spine and the position and dimensions of individual vertebrae in German Shepherds 德国牧羊犬腰椎缺损与个体椎体位置和尺寸的关系
IF 0.4 4区 农林科学 Q3 Veterinary Pub Date : 2023-01-01 DOI: 10.21521/mw.6792
M. Dzierzęcka, S. Paśko, I. Wadowska, T. Kowaluk, I. Łuszczewska-Sierakowska, A. Charuta
The German Shepherd is among the breeds most prone to pathologies of the lumbosacral spine. The aim of the study was to examine how the presence of spine pathology affects the shape of the lumbar spine and dimensions of individual vertebrae. Mathematical analysis consisted of three measurements for each lumbar vertebra. Based on the analysis, it was concluded that there was a correlation between the height of the first five vertebrae and the occurrence of the lumbosacral transitional vertebra (LTV). It was also shown that spondylosis manifested most often with a change in the distance between individual lumbar vertebrae. There was no correlation between the incidence of spondylosis and the height of the vertebral canal. In conclusion, the presence of a LTV significantly changes the dimensions of other vertebrae in the lumbar spine, which can lead to other pathological changes in the vertebral column.
德国牧羊犬是最容易出现腰骶脊柱病变的犬种之一。该研究的目的是检查脊柱病理的存在如何影响腰椎的形状和单个椎骨的尺寸。数学分析包括对每个腰椎进行三次测量。通过分析,认为前5节椎骨的高度与腰骶过渡椎(LTV)的发生有一定的相关性。研究还表明,颈椎病最常表现为个体腰椎之间距离的变化。椎管的高度与颈椎病的发病率没有相关性。总之,LTV的存在显著改变了腰椎其他椎骨的尺寸,从而导致脊柱的其他病理改变。
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引用次数: 0
Combating animal infectious diseases in Poland in the years 1918-1939 on the example of rinderpest and foot and mouth disease. Legal and administrative aspect 1918-1939年在波兰防治动物传染病,以牛瘟和口蹄疫为例。法律和行政方面
IF 0.4 4区 农林科学 Q3 Veterinary Pub Date : 2023-01-01 DOI: 10.21521/mw.6794
M. Welz, Renata Kondrat, Przemysław Łoś, Janusz Bogdan, K. Anusz
The regaining of independence by Poland in 1918 brought freedom, but it also posed many challenges for the state administration, including the reconstruction of a uniform veterinary service. It was not an easy task, considering that already in early 1920 an epizootic of rinderpest crossed the Caucasus and spread in the European part of Soviet Russia, reaching eastern Poland in September of that year. A modern legal framework for combating contagious animal diseases was successfully established within merely ten years. A breakthrough in the field of law regarding the eradication of infectious animal diseases was the Regulation of the President of the Republic of Poland of August 22, 1927 on combating contagious animal diseases. It was the first legal act of this type in the country and one of the most modern regulations of its kind in Europe at that time. The list of infectious diseases covered by the obligation to report and control included rinderpest, contagious bovine pleuropneumonia, foot-and-mouth disease, anthrax, blackleg disease, game and cattle plague, tuberculosis of cattle in the open form (lungs, udder, uterus and intestines), glanders of solipeds, sheep pox, dourine, coital exanthema of equines and cattle, scabies of solipeds and sheep, rabies, swine fever and plague, swine erysipelas, poultry cholera (pasterellosis) and chicken plague. The article presents the rules of combating infectious animal diseases in the example of rinderpest and foot-and-mouth disease, which were characteristic of the period of the Second Polish Republic.
1918年波兰重新获得独立带来了自由,但也给国家行政管理带来了许多挑战,包括重建统一的兽医服务。这不是一件容易的事,因为早在1920年初,牛瘟就已经越过高加索,蔓延到苏俄的欧洲部分,并于当年9月到达波兰东部。在短短十年内,就成功地建立了防治动物传染病的现代法律框架。在消除动物传染病的法律领域,1927年8月22日颁布的《波兰共和国总统关于防治动物传染病的条例》是一个突破。这是该国第一个此类法律行为,也是当时欧洲同类法规中最现代的法规之一。报告和控制义务所涵盖的传染病清单包括牛瘟、传染性牛胸膜肺炎、口蹄疫、炭疽、黑腿病、野味和牛鼠疫、牛的开放性结核病(肺、乳房、子宫和肠道)、固体腺、羊痘、尿疹、马和牛的阴道癣、固体和羊的疥疮、狂犬病、猪瘟和鼠疫、猪丹毒、家禽霍乱(巴斯德菌病)和鸡鼠疫。本文以波兰第二共和国时期特有的牛瘟和口蹄疫为例,介绍了防治动物传染病的规则。
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引用次数: 0
Access to public information regarding laboratory animals: law and ethics 获取有关实验动物的公共信息:法律和伦理
IF 0.4 4区 农林科学 Q3 Veterinary Pub Date : 2023-01-01 DOI: 10.21521/mw.6798
A. Dzikowski
Normative regulations concerning the use of animals in experiments and education raise numerous controversies and much social discussion. One of the aspects of implementing this form of social activity is the use of constitutional, subjective right of access to public information. Competent public administration authorities, and administrative courts encounter numerous difficulties related to requests for information. Attempts to assess, evaluate, and verify whether the applicants’ goals and intentions are consistent with the axiological premises of the statutory norms occur. The study reveals veterinary, legal, and moral aspects of requests for access to public information related to the use of animals in testing and teaching procedures. The controversial concept of abuse/misuse of the subjective right to access information is presented in a critical, multifaceted way. Obligations of professional ethics of veterinary surgeons concerning laboratory animals and public information related to them are discussed. The effectiveness and consistency of normative regulations are assessed. Interpretational postulates are indicated.
关于在实验和教育中使用动物的规范性规定引起了许多争议和许多社会讨论。实施这种社会活动形式的一个方面是利用宪法规定的获取公共信息的主观权利。主管公共行政当局和行政法院在要求提供资料方面遇到许多困难。试图评估、评价和验证申请人的目标和意图是否与法定规范的价值论前提一致。该研究揭示了获取有关在试验和教学过程中使用动物的公共信息的请求的兽医、法律和道德方面的问题。滥用/误用获取信息的主观权利这一有争议的概念以一种关键的、多方面的方式提出。讨论了兽医对实验动物的职业道德义务以及与实验动物相关的公共信息。评估规范性法规的有效性和一致性。说明了解释性假设。
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引用次数: 0
Key role of the local veterinary administration in combating infectious diseases of wild animals: New duties vs. old habits in the context of the Animal Health Law 地方兽医管理部门在防治野生动物传染病方面的关键作用:《动物卫生法》背景下的新职责与旧习惯
IF 0.4 4区 农林科学 Q3 Veterinary Pub Date : 2023-01-01 DOI: 10.21521/mw.6807
A. Dzikowski, K. Anusz, Janusz Bogdan
Regulation (EU) 2016/429 of the European Parliament and of the Council of 9 March 2016 on transmissible animal diseases and amending and repealing certain acts in the field of animal health, ‘Animal Health Law’, changed the rules for combating infectious diseases of animals, in particular of non-domesticated species. An in-depth legal research by the authors revealed certain features of combat against transmissible diseases in wild animals. New normative regulations are presented, confronted, and evaluated. The authors conclude that the European law has changed the content of national statutes, although their literal wording has remained unchanged. Different definitions and designations of veterinary surgeons in the Animal Health Law are discussed. An algorithm of conduct aimed at the uniformity of the work of public veterinary administration is presented. New duties and the need for high legal competences of the District Veterinary Officer are discussed. The effectiveness and consistency of the regulations are assessed. Interpretational and legislative postulates are indicated.
2016年3月9日欧洲议会和理事会第(EU) 2016/429号关于动物传染性疾病以及修订和废除动物卫生领域某些行为的条例,即“动物卫生法”,改变了防治动物,特别是非驯化物种传染病的规则。作者进行了深入的法律研究,揭示了野生动物防治传染病的某些特点。新的规范规定被提出、面对和评估。作者的结论是,欧洲法律改变了国家法规的内容,尽管它们的字面措辞保持不变。讨论了《动物卫生法》中兽医的不同定义和称谓。提出了一种旨在统一公共兽医管理工作的行为算法。讨论了地区兽医官的新职责和高度法律权限的必要性。对法规的有效性和一致性进行了评估。说明了解释和立法的规定。
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引用次数: 0
Modified Ventral Femoral Head and Neck Ostectomy: A Canine Cadaver Study 改良股骨头颈腹侧骨切除术:犬尸体研究
4区 农林科学 Q3 Veterinary Pub Date : 2023-01-01 DOI: 10.21521/mw.6832
PIOTR TRĘBACZ, JAN FRYMUS, MICHAŁ CZOPOWICZ, IZABELLA JOŃSKA, RAFAŁ LENGLING, MAREK GALANTY
The aim of this study was to assess the accuracy and efficiency of performing modified ventral femoral head and neck ostectomy (mFHNO) after measuring the ideal femoral head and neck ostectomy angle (iFHNOA) on preoperative radiographs of the hip joints. Our modification of the FHNO consisted of removal of the femoral head and neck in stages. The study included cadavers of 10 adult large breed dogs (5 mongrel dogs, 4 German shepherds and 1 Gordon setter), 5 males and 5 females, weighing between 25 and 45 kg (36.2 ± 6.8 kg). There were 18 femoral head and neck ostectomies: 8 left and 10 right. The mean difference (d) between the achieved postoperative ostectomy angle and iFHNOA (± standard deviation) was 1.9° ± 7.8° (95% confidence interval: –4.6°; 8.4°) in the left limb and 0° ± 6.8° (95% confidence interval: –4.9°; 4.9°) in the right limb. Both mean d value were not statistically significantly different from 0° (p = 0.518 in the left limb and p = 0.999 in the right limb), which means that the ostectomy angles obtained by the modified method were performed nearly ideally in each case.
本研究的目的是在术前髋关节x线片测量理想股骨头颈截骨角度(iFHNOA)后,评估实施改良腹侧股骨头颈截骨术(mFHNO)的准确性和效率。我们对FHNO的改良包括分阶段切除股骨头和股颈。该研究包括10只成年大型犬的尸体(5只杂种狗,4只德国牧羊犬和1只戈登塞特犬),5只雄性和5只雌性,体重在25至45公斤(36.2±6.8公斤)之间。股骨头颈切除术18例,左侧8例,右侧10例。术后实现的截骨角度与iFHNOA之间的平均差(d)(±标准差)为1.9°±7.8°(95%可信区间:-4.6°;左肢体8.4°)和0°±6.8°(95%可信区间:-4.9°;4.9°)。两者的平均d值均与0°无统计学差异(左肢p = 0.518,右肢p = 0.999),说明改进方法获得的截骨角度在两种情况下均接近理想。
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引用次数: 0
Current Energy and Lipid Metabolism Biomarkers in Sheep with Subclinical And Clinical Pregnancy Toxemia 亚临床和临床妊娠毒血症绵羊的能量和脂质代谢生物标志物
IF 0.4 4区 农林科学 Q3 Veterinary Pub Date : 2023-01-01 DOI: 10.21521/mw.6733
Merve Öztürk, N. Mamak
The aim of this study was to evaluate the relationship between existing metabolic biomarkers (asprosin, FABP1, PPARα and FGF21) and clinical parameters and to determine their role in the early diagnosis of sheep pregnancy toxemia. In the study, 70 Akkaraman sheep aged three to five years in the last three weeks of pregnancy were divided into three groups: healthy group (n = 20), subclinical group (n = 30), and clinical group (n = 20). Clinical examination (body temperature, respiratory rate, and heart rate), blood serum biochemistry, and ELISA analyses were performed. In sheep with clinical and subclinical pregnancy toxemia, serum PPARα, β-HBA, NEFA, HbA1c, HDL, triglyceride, creatinine, and phosphorus levels were statistically higher, whereas serum glucose and LDL levels were lower than in healthy sheep. However, serum FGF21, AST, ALT, and VLDL values were not statistically different between the three groups. In addition, serum asprosin and FABP1 levels were higher in the subclinical group than in the clinical group. Thus it was concluded that serum asprosin, FABP1, and PPARα findings could be useful in evaluating lipid and energy metabolism in subclinical and clinical forms of pregnancy toxemia. Since this study was based on blood samples from individually reared sheep herds, many environmental factors (e.g., housing, nutrition, and population density) could not be considered. For this reason, it is thought that there is a need for experimental studies in which environmental variables can be controlled.
本研究的目的是评估绵羊妊娠毒血症的现有代谢生物标志物(asprosin、FABP1、PPARα和FGF21)与临床参数的关系,并确定其在妊娠毒血症早期诊断中的作用。在这项研究中,70只3 - 5岁、妊娠最后三周的阿卡拉曼羊被分为三组:健康组(n = 20)、亚临床组(n = 30)和临床组(n = 20)。临床检查(体温、呼吸频率、心率)、血清生化、ELISA分析。临床和亚临床妊娠毒血症羊血清PPARα、β-HBA、NEFA、HbA1c、HDL、甘油三酯、肌酐和磷水平均高于健康羊,而血清葡萄糖和LDL水平低于健康羊。血清FGF21、AST、ALT、VLDL值三组间差异无统计学意义。此外,亚临床组血清asprosin和FABP1水平高于临床组。由此得出结论,血清asprosin、FABP1和PPARα的检测结果可用于评估亚临床和临床形式妊娠毒血症的脂质和能量代谢。由于这项研究是基于单独饲养的羊群的血液样本,因此许多环境因素(如住房、营养和人口密度)无法考虑在内。因此,人们认为有必要进行环境变量可以控制的实验研究。
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