R. Mordak, Z. Dobrzański, J. Popiel, R. Kupczyński
The aim of the study was to assess the values of and correlations between selected markers of acid-base balance and electrolytes in the blood of late lactation cows, such as pH, pCO2, pO2, HCO3 –, BE, BE(B), Na+, K+, Cl–, as well as selected parameters of milk, such as the number of somatic cells (SCC), colony forming units (CFU), milk fat (MF), milk protein (MP), milk lactose (ML), milk solids, milk solids non-fat (SNF), and current milk production. Blood and milk samples were collected at the same time from 11 clinically healthy milking cows in the later lactation period. Those 11 selected cows were examined once a day for 3 consecutive days resulting in 33 sets of blood and milk samples used for laboratory and statistical analyses. Significant positive correlations were observed between pH and HCO3 –, pH and BE, pH and BE(B), pCO2 and HCO3 –, pCO2 and BE, pCO2 and BE(B), HCO3 – and BE, HCO3 – and BE(B), BE and BE(B), Na+ and Cl–, Na+ and milk production, Cl– and milk production, and Cl– and MP. Significant negative correlations were observed between pH and K+, pH and Cl–, pCO2 and Cl–, HCO3 – and Cl–, BE and Cl–, and BE(B) and Cl–. The increase in blood pH was significantly negatively correlated with the current milk production, while the increase in HCO3 – or pCO2 was associated with a significant decrease in the amount of protein in milk. These relations can indicate a significant negative influence of blood acid-base imbalance on the quantity and quality of cow’s milk. The repeatable diagnostic model presented here proved to be a very useful tool for a detailed assessment of selected higly sensitive parameters of internal homeostasis in productive dairy cows.
{"title":"Analysis of Correlations between Selected Blood Markers of Acid-Base Balance, Blood Electrolytes, and Milk Components in Dairy Cows during Late Lactation","authors":"R. Mordak, Z. Dobrzański, J. Popiel, R. Kupczyński","doi":"10.21521/mw.6787","DOIUrl":"https://doi.org/10.21521/mw.6787","url":null,"abstract":"The aim of the study was to assess the values of and correlations between selected markers of acid-base balance and electrolytes in the blood of late lactation cows, such as pH, pCO2, pO2, HCO3 –, BE, BE(B), Na+, K+, Cl–, as well as selected parameters of milk, such as the number of somatic cells (SCC), colony forming units (CFU), milk fat (MF), milk protein (MP), milk lactose (ML), milk solids, milk solids non-fat (SNF), and current milk production. Blood and milk samples were collected at the same time from 11 clinically healthy milking cows in the later lactation period. Those 11 selected cows were examined once a day for 3 consecutive days resulting in 33 sets of blood and milk samples used for laboratory and statistical analyses. Significant positive correlations were observed between pH and HCO3 –, pH and BE, pH and BE(B), pCO2 and HCO3 –, pCO2 and BE, pCO2 and BE(B), HCO3 – and BE, HCO3 – and BE(B), BE and BE(B), Na+ and Cl–, Na+ and milk production, Cl– and milk production, and Cl– and MP. Significant negative correlations were observed between pH and K+, pH and Cl–, pCO2 and Cl–, HCO3 – and Cl–, BE and Cl–, and BE(B) and Cl–. The increase in blood pH was significantly negatively correlated with the current milk production, while the increase in HCO3 – or pCO2 was associated with a significant decrease in the amount of protein in milk. These relations can indicate a significant negative influence of blood acid-base imbalance on the quantity and quality of cow’s milk. The repeatable diagnostic model presented here proved to be a very useful tool for a detailed assessment of selected higly sensitive parameters of internal homeostasis in productive dairy cows.","PeriodicalId":49017,"journal":{"name":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","volume":"164 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68107857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aging is a major risk factor for cognitive decline, such as Alzheimer’s disease (AD). Sheep’s milk may play a role in the prevention of neurodegenerative diseases because of its high content of functional peptides, such as lactoferrin. Lactoferrin can be used in AD both as an active therapeutic compound and as a drug nanocarrier. Compared to cow’s and goat’s milks, sheep’s milk contains the highest content of isomers of conjugated linoleic acid (CLA). The c-9, t-11-CLA isomers reduce the pathological changes occurring in AD mainly by reducing the amount of amyloid-β aggregates in brain tissues. Sheep’s milk also contains higher concentrations of vitamin D and B-group vitamins, which have neuroprotective effects. This review summarizes the current knowledge on sheep’s milk and its products, with a special focus on its role in the prevention of neurodegenerative diseases.
{"title":"Role of bioactive substances in sheep's milk and its products in the prevention of neurodegenerative diseases","authors":"Zuzanna Flis, E. Molik","doi":"10.21521/mw.6796","DOIUrl":"https://doi.org/10.21521/mw.6796","url":null,"abstract":"Aging is a major risk factor for cognitive decline, such as Alzheimer’s disease (AD). Sheep’s milk may play a role in the prevention of neurodegenerative diseases because of its high content of functional peptides, such as lactoferrin. Lactoferrin can be used in AD both as an active therapeutic compound and as a drug nanocarrier. Compared to cow’s and goat’s milks, sheep’s milk contains the highest content of isomers of conjugated linoleic acid (CLA). The c-9, t-11-CLA isomers reduce the pathological changes occurring in AD mainly by reducing the amount of amyloid-β aggregates in brain tissues. Sheep’s milk also contains higher concentrations of vitamin D and B-group vitamins, which have neuroprotective effects. This review summarizes the current knowledge on sheep’s milk and its products, with a special focus on its role in the prevention of neurodegenerative diseases.","PeriodicalId":49017,"journal":{"name":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68108513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the article is to analyze the statements of the Church of England on the ethical aspects of conducting experiments on animals. From the rich teaching of this community on eco-theological topics, several documents were selected that deal with these issues. They show the evolution and development of teaching and an attempt to relate moral norms to the legislation of the United Kingdom. The Church of England recognizes the need to include the 3R principle in scientific research, supplementing it with the postulate of human responsibility for animals.
{"title":"Animal experiments in statements of the Church of England","authors":"Krzysztof Smykowski","doi":"10.21521/mw.6809","DOIUrl":"https://doi.org/10.21521/mw.6809","url":null,"abstract":"The aim of the article is to analyze the statements of the Church of England on the ethical aspects of conducting experiments on animals. From the rich teaching of this community on eco-theological topics, several documents were selected that deal with these issues. They show the evolution and development of teaching and an attempt to relate moral norms to the legislation of the United Kingdom. The Church of England recognizes the need to include the 3R principle in scientific research, supplementing it with the postulate of human responsibility for animals.","PeriodicalId":49017,"journal":{"name":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68110007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The gut microbiome of animals varies by species, age, sex, and nutrition. The content of nutrients in the diet as well as feed additives (synbiotics, eubiotics, phytobiotics) determine the quantity and quality of the intestinal flora. The ban on the use of antibiotic growth promoters (AGP) has led to the search for various effective alternatives that affect the gut microbiome, animal health and production results. It has been shown that the use of fermented feeds, beneficially modifying the composition of the intestinal flora, can be a significant alternative to AGP in the production and breeding of monogastric animals. The use of fermented feeds in wet nutrition or in dry mixtures contributes to the improvement of the nutritional value of the diet by increasing the content of protein and exogenous amino acids and polyunsaturated fatty acids, reducing the content of anti-nutritional substances (glucosinolates, phytates, β-glycinates) in feeds (rapeseed, soybean, lupins, horse bean) and increasing the concentration of beneficial microorganisms (Bacillus and Lactobacillus) and their products (bacteriocins, organic acids).
{"title":"Influence of fermented feeds on the microbiome of the gastrointestinal tract and efficiency in animal nutrition","authors":"E. Grela, R. Klebaniuk","doi":"10.21521/mw.6811","DOIUrl":"https://doi.org/10.21521/mw.6811","url":null,"abstract":"The gut microbiome of animals varies by species, age, sex, and nutrition. The content of nutrients in the diet as well as feed additives (synbiotics, eubiotics, phytobiotics) determine the quantity and quality of the intestinal flora. The ban on the use of antibiotic growth promoters (AGP) has led to the search for various effective alternatives that affect the gut microbiome, animal health and production results. It has been shown that the use of fermented feeds, beneficially modifying the composition of the intestinal flora, can be a significant alternative to AGP in the production and breeding of monogastric animals. The use of fermented feeds in wet nutrition or in dry mixtures contributes to the improvement of the nutritional value of the diet by increasing the content of protein and exogenous amino acids and polyunsaturated fatty acids, reducing the content of anti-nutritional substances (glucosinolates, phytates, β-glycinates) in feeds (rapeseed, soybean, lupins, horse bean) and increasing the concentration of beneficial microorganisms (Bacillus and Lactobacillus) and their products (bacteriocins, organic acids).","PeriodicalId":49017,"journal":{"name":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68110213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dirofilaria immitis (D. immitis) is a vector-mediated zoonotic parasite that can cause significant cardiopulmonary problems in humans and animals. Although dogs are the main hosts of the parasite, in recent years, its importance has been increasing in cats, especially in endemic areas. This study aims to determine the occurrence of D. immitis infection in cats in the Aegean region, Turkey. The animal material of the study consisted of 200 cats of different breeds and ages (at least 1 year old) and both sexes. The cats were tested for D. immitis and Feline Immunodeficiency Virus (FIV) antibodies and Feline Leukaemia Virus (FeLV) antigen with commercial immuno-chromatographic test kits. In addition, blood samples were examined for microfilariae using a direct blood smear technique and the modified Knott’s test. Out of 200 cats, one (0.5%) cat was seropositive for D. immitis, 15 (7.5%) cats were seropositive for FIV, and one (0.5%) cat was seropositive for FeLV. One cat seropositive for D. immitis was showing signs of active heartworm infection. All samples were found negative for microfilariae by direct blood examination and the modified Knott’s method. This study is the first report of the occurrence of D. immitis in the Aegean Region, with a seroprevalence of 0.5%. Also, it provides evidence that cats in the Aegean region are at risk of becoming infected with D. immitis.
{"title":"Occurrence of Dirofilaria immitis in cats from the Aegean region in Turkey","authors":"G. E. Tuna, K. Aksoy, C. Ay","doi":"10.21521/mw.6706","DOIUrl":"https://doi.org/10.21521/mw.6706","url":null,"abstract":"Dirofilaria immitis (D. immitis) is a vector-mediated zoonotic parasite that can cause significant cardiopulmonary problems in humans and animals. Although dogs are the main hosts of the parasite, in recent years, its importance has been increasing in cats, especially in endemic areas. This study aims to determine the occurrence of D. immitis infection in cats in the Aegean region, Turkey. The animal material of the study consisted of 200 cats of different breeds and ages (at least 1 year old) and both sexes. The cats were tested for D. immitis and Feline Immunodeficiency Virus (FIV) antibodies and Feline Leukaemia Virus (FeLV) antigen with commercial immuno-chromatographic test kits. In addition, blood samples were examined for microfilariae using a direct blood smear technique and the modified Knott’s test. Out of 200 cats, one (0.5%) cat was seropositive for D. immitis, 15 (7.5%) cats were seropositive for FIV, and one (0.5%) cat was seropositive for FeLV. One cat seropositive for D. immitis was showing signs of active heartworm infection. All samples were found negative for microfilariae by direct blood examination and the modified Knott’s method. This study is the first report of the occurrence of D. immitis in the Aegean Region, with a seroprevalence of 0.5%. Also, it provides evidence that cats in the Aegean region are at risk of becoming infected with D. immitis.","PeriodicalId":49017,"journal":{"name":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68101015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Dzierzęcka, M. Jaworski, M. Komosa, I. Łuszczewska-Sierakowska, Anna CHARUTA5
The third metacarpal (MC3) bone, along with the proximal phalanx, is one of the bones that are most prone to injury in sport horses. To date, no detailed analysis has been conducted that would compare the strength parameters of bilateral MC3 bones taking into account differences depending on the site of measurement. The aim of the study was to compare strength parameters between the left and right MC3 bones in horses at 10%, 20%, 30%, 40%, 50%, 60%, 70% and 80% of the bone length using peripheral quantitative computed tomography (pQCT). The research material comprised isolated bilateral MC3 bones from 21 horses (age range: 3-27 years). The structure of these bones was measured using high-resolution pQCT. The following bone parameters were determined: polar strength strain index, strength strain index X and strength strain index Y. The computed tomographic analysis of the MC3 bones was carried out at sections from 10% to 80% (every 10%) of the bone length. The statistical analysis showed that in most cases the strength parameters calculated using pQCT were significantly higher for the right MC3 bones at 10%, 20% and 50% of the bone length, i.e. at the proximal metaphysis and at the proximal diaphysis. However, strength parameters measured at 60% and 80% of the diaphyseal length, i.e. at the distal diaphysis, were significantly higher for the left MC3 bones. Further studies of the MC3 bones parameters should focus on the vicinity of the proximal metaphysis and at the proximal diaphysis.
{"title":"Comparison of the strength parameters of the bilateral third metacarpal bones in horses by pQCT","authors":"M. Dzierzęcka, M. Jaworski, M. Komosa, I. Łuszczewska-Sierakowska, Anna CHARUTA5","doi":"10.21521/mw.6720","DOIUrl":"https://doi.org/10.21521/mw.6720","url":null,"abstract":"The third metacarpal (MC3) bone, along with the proximal phalanx, is one of the bones that are most prone to injury in sport horses. To date, no detailed analysis has been conducted that would compare the strength parameters of bilateral MC3 bones taking into account differences depending on the site of measurement. The aim of the study was to compare strength parameters between the left and right MC3 bones in horses at 10%, 20%, 30%, 40%, 50%, 60%, 70% and 80% of the bone length using peripheral quantitative computed tomography (pQCT). The research material comprised isolated bilateral MC3 bones from 21 horses (age range: 3-27 years). The structure of these bones was measured using high-resolution pQCT. The following bone parameters were determined: polar strength strain index, strength strain index X and strength strain index Y. The computed tomographic analysis of the MC3 bones was carried out at sections from 10% to 80% (every 10%) of the bone length. The statistical analysis showed that in most cases the strength parameters calculated using pQCT were significantly higher for the right MC3 bones at 10%, 20% and 50% of the bone length, i.e. at the proximal metaphysis and at the proximal diaphysis. However, strength parameters measured at 60% and 80% of the diaphyseal length, i.e. at the distal diaphysis, were significantly higher for the left MC3 bones. Further studies of the MC3 bones parameters should focus on the vicinity of the proximal metaphysis and at the proximal diaphysis.","PeriodicalId":49017,"journal":{"name":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68102128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zbigniew Bełkot, Izabela Pietrzyk, Julia Jednous, Łukasz Drozd, M. Ziomek
Chronic wasting disease (CWD) is a lethal infectious neurodegenerative disease, the etiological factor of which is the infectious prion protein. Infections affect animals of the deer family (Cervidae). The aim of the study was to analyze the results of monitoring for the presence of CWD in the years 2018-2020 carried out by the Veterinary Inspectorate in Poland. The aim of the monitoring program in force in 2018-2020 was to assess the epizootic situation of CWD in Poland and other European countries, where no cases of the disease have been detected so far. Six species of deer were monitored: Eurasian tundra reindeer, Finnish forest reindeer, roe deer, elk, red deer, and white-tailed deer. Samples for the study were collected by veterinarians and trained persons and came from a latch within the medulla, retropharyngeal lymph nodes, tonsils, or other lymph nodes in the head. During the three-year CWD monitoring in Poland, a total of 3541 samples from animals from the deer family were examined. With regard to farmed and captive animals, the samples came from 225 red deer and 1 clump, and in the case of wild animals, the study included 192 elk, 2703 roe deer, and 420 red deer. In 2018, 1141 animals were tested: 23 farm-kept noble deer and 42 elk, 886 roe deer, and 190 wild-living noble deer. In 2019, the monitoring covered 1246 animals: 115 red deer and 1 female elk were farm animals, and the remaining 83 elk, 902 roe deer, and 145 red deer were free-living. In the last year of the program, 1154 animals were tested: 87 farmed red deer and 67 wild elk, 915 roe deer, and 85 wild deer. During the monitoring period, no cases of chronic wasting disease (CWD) of cervids were detected in Poland. The program officially ended at the end of December 2020 and was not extended because of the absence of CWD-positive results.
{"title":"Monitoring of chronic wasting disease in deer in Poland between 2018 and 2020","authors":"Zbigniew Bełkot, Izabela Pietrzyk, Julia Jednous, Łukasz Drozd, M. Ziomek","doi":"10.21521/mw.6721","DOIUrl":"https://doi.org/10.21521/mw.6721","url":null,"abstract":"Chronic wasting disease (CWD) is a lethal infectious neurodegenerative disease, the etiological factor of which is the infectious prion protein. Infections affect animals of the deer family (Cervidae). The aim of the study was to analyze the results of monitoring for the presence of CWD in the years 2018-2020 carried out by the Veterinary Inspectorate in Poland. The aim of the monitoring program in force in 2018-2020 was to assess the epizootic situation of CWD in Poland and other European countries, where no cases of the disease have been detected so far. Six species of deer were monitored: Eurasian tundra reindeer, Finnish forest reindeer, roe deer, elk, red deer, and white-tailed deer. Samples for the study were collected by veterinarians and trained persons and came from a latch within the medulla, retropharyngeal lymph nodes, tonsils, or other lymph nodes in the head. During the three-year CWD monitoring in Poland, a total of 3541 samples from animals from the deer family were examined. With regard to farmed and captive animals, the samples came from 225 red deer and 1 clump, and in the case of wild animals, the study included 192 elk, 2703 roe deer, and 420 red deer. In 2018, 1141 animals were tested: 23 farm-kept noble deer and 42 elk, 886 roe deer, and 190 wild-living noble deer. In 2019, the monitoring covered 1246 animals: 115 red deer and 1 female elk were farm animals, and the remaining 83 elk, 902 roe deer, and 145 red deer were free-living. In the last year of the program, 1154 animals were tested: 87 farmed red deer and 67 wild elk, 915 roe deer, and 85 wild deer. During the monitoring period, no cases of chronic wasting disease (CWD) of cervids were detected in Poland. The program officially ended at the end of December 2020 and was not extended because of the absence of CWD-positive results.","PeriodicalId":49017,"journal":{"name":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68102232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Equine endometrosis is a chronic degenerative condition with progressive fibrosis that forms in the endometrial stroma and around the endometrial glands. Equine endometrosis causes histological changes and alterations in the secretory function of endometrial cells. These alterations lead to changes in the endometrium and early pregnancy dysfunction. Thus, this condition is one of the main causes of subfertility in mares, resulting in enormous economic losses to the horse-breeding industry. In general, fibrosis is characterized by excessive deposition of extracellular matrix (ECM) components. It destroys normal tissue architecture and compromises tissue function. The cellular and molecular events associated with the development of tissue fibrosis are complex and poorly understood. In recent years, research has been carried out on the involvement of immune cells and their products in the pathogenesis of endometrosis in mares. These studies focused mainly on the role of cytokines, such as transforming growth factor (TGF)-β1, interleukin (IL) 6, IL-1β, prostaglandin (PG), and components of neutrophil extracellular traps (NET), such as myeloperoxidase, cathepsin G, and elastase, in the most important processes associated with the development of endometrosis. The results showed that TGF-β1, IL-1β, IL-6, and PGs act on myofibroblast differentiation, fibroblast proliferation, deposition of ECM protein, and expression of matrix metalloproteinases (MMPs), and their inhibitors. It suggests their involvement in processes associated with pathological endometrial remodeling. Moreover, the response of endometrial tissue to selected factors changes according to the stage of endometrosis. The action of inflammatory mediators on processes related to the development of fibrosis shows a connection between inflammation and endometrosis.
{"title":"The role of inflammatory mediators in the development of endometrosis in mare","authors":"SZÓSTEK-MIODUCHOWSKA Anna, Wojtowicz-Krawiec Anna, Sadowska Agnieszka, Skarżyński Dariusz","doi":"10.21521/mw.6722","DOIUrl":"https://doi.org/10.21521/mw.6722","url":null,"abstract":"Equine endometrosis is a chronic degenerative condition with progressive fibrosis that forms in the endometrial stroma and around the endometrial glands. Equine endometrosis causes histological changes and alterations in the secretory function of endometrial cells. These alterations lead to changes in the endometrium and early pregnancy dysfunction. Thus, this condition is one of the main causes of subfertility in mares, resulting in enormous economic losses to the horse-breeding industry. In general, fibrosis is characterized by excessive deposition of extracellular matrix (ECM) components. It destroys normal tissue architecture and compromises tissue function. The cellular and molecular events associated with the development of tissue fibrosis are complex and poorly understood. In recent years, research has been carried out on the involvement of immune cells and their products in the pathogenesis of endometrosis in mares. These studies focused mainly on the role of cytokines, such as transforming growth factor (TGF)-β1, interleukin (IL) 6, IL-1β, prostaglandin (PG), and components of neutrophil extracellular traps (NET), such as myeloperoxidase, cathepsin G, and elastase, in the most important processes associated with the development of endometrosis. The results showed that TGF-β1, IL-1β, IL-6, and PGs act on myofibroblast differentiation, fibroblast proliferation, deposition of ECM protein, and expression of matrix metalloproteinases (MMPs), and their inhibitors. It suggests their involvement in processes associated with pathological endometrial remodeling. Moreover, the response of endometrial tissue to selected factors changes according to the stage of endometrosis. The action of inflammatory mediators on processes related to the development of fibrosis shows a connection between inflammation and endometrosis.","PeriodicalId":49017,"journal":{"name":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68102266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Natalia Shkavro, A. Kozubska-Sobocińska, D. Rubiś, Alisa Shkavro, M. Babicz, O. Shcherbak, I. Łuszczewska-Sierakowska
The aim of the research was to evaluate the genetic structure of Ukrainian Holstein cattle (n = 150) of the state enterprise Chayka (Kiev region, Ukraine) on the basis of the analysis of 12 microsatellite DNA loci and 3 QTL genes: k-casein (CSN3), prolactin (PRL), and fatty acid synthase (FASN). A total of 93 allelic variants were identified, and the largest number of alleles were found for TGLA53, TGLA122 and TGLA227 loci, the mean PIC = 0.693, and the heterozygosity index for all loci was 0.7314. The greatest number of effective alleles per locus were found for the BM1824 locus. Based on association analyses, complex genotypes were established for the three genes (CSN3, PRL, FASN). It was found that the AAPRL AACSN3 AAFASN genotype determined the highest milk yield (8351 kg / lactation) and the protein content in milk (3.10%), whereas the AAPRL ABCSN3 GGFASN genotype determined the highest level of fat content in milk (3.80%).
{"title":"Ukrainian Holstein cattle DNA polymorphism study","authors":"Natalia Shkavro, A. Kozubska-Sobocińska, D. Rubiś, Alisa Shkavro, M. Babicz, O. Shcherbak, I. Łuszczewska-Sierakowska","doi":"10.21521/mw.6726","DOIUrl":"https://doi.org/10.21521/mw.6726","url":null,"abstract":"The aim of the research was to evaluate the genetic structure of Ukrainian Holstein cattle (n = 150) of the state enterprise Chayka (Kiev region, Ukraine) on the basis of the analysis of 12 microsatellite DNA loci and 3 QTL genes: k-casein (CSN3), prolactin (PRL), and fatty acid synthase (FASN). A total of 93 allelic variants were identified, and the largest number of alleles were found for TGLA53, TGLA122 and TGLA227 loci, the mean PIC = 0.693, and the heterozygosity index for all loci was 0.7314. The greatest number of effective alleles per locus were found for the BM1824 locus. Based on association analyses, complex genotypes were established for the three genes (CSN3, PRL, FASN). It was found that the AAPRL AACSN3 AAFASN genotype determined the highest milk yield (8351 kg / lactation) and the protein content in milk (3.10%), whereas the AAPRL ABCSN3 GGFASN genotype determined the highest level of fat content in milk (3.80%).","PeriodicalId":49017,"journal":{"name":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68102350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rifkat Fairushin, S. Shakirova, R. Ganieva, G. Shakirova
Unbalanced nutrition as well as diseases of infectious and non-infectious etiology lead to a decrease in production and a significant loss of farm animals and birds. To mitigate these problems, various probiotic drugs (probiotics) are introduced into the diets of birds and animals for preventive purposes. The aim of the research was to study the effect of the Vitafort probiotic, containing antagonistic bacteria of the endophytic strain Bacillus subtilis B11, on the body of broiler chickens. The effect of Vitafort was assessed by measuring the live weight and average daily growth of birds. In addition, a morphological study of blood was carried out and the content of Bacillus subtilis in the small intestine was determined. It was found that the endophytic strain Bacillus subtilis B11 improved the intestinal microflora. Thus, on day 5 of its use, the number of microorganisms varied from 4.1 × 104 to 8.2 × 104 CFU/g, and on day 42 the same number varied from 4 × 108 to 5.8 × 108 CFU/g on day 42, the contents of red blood cells, haemoglobin, and white blood cells in the blood of chickens from the experimental group were higher by 6.44%, 3.44%, and 2.11%, respectively, than they were in the control group. Considering the above facts, it can be assumed that the absorption of nutrients in the intestine improved, and metabolic processes were activated due to an increase in the transport abilities of red blood cells.
{"title":"Effectiveness of Bacillus subtilis (Vitafort) probiotic used for raising broiler chickens","authors":"Rifkat Fairushin, S. Shakirova, R. Ganieva, G. Shakirova","doi":"10.21521/mw.6621","DOIUrl":"https://doi.org/10.21521/mw.6621","url":null,"abstract":"Unbalanced nutrition as well as diseases of infectious and non-infectious etiology lead to a decrease in production and a significant loss of farm animals and birds. To mitigate these problems, various probiotic drugs (probiotics) are introduced into the diets of birds and animals for preventive purposes. The aim of the research was to study the effect of the Vitafort probiotic, containing antagonistic bacteria of the endophytic strain Bacillus subtilis B11, on the body of broiler chickens. The effect of Vitafort was assessed by measuring the live weight and average daily growth of birds. In addition, a morphological study of blood was carried out and the content of Bacillus subtilis in the small intestine was determined. It was found that the endophytic strain Bacillus subtilis B11 improved the intestinal microflora. Thus, on day 5 of its use, the number of microorganisms varied from 4.1 × 104 to 8.2 × 104 CFU/g, and on day 42 the same number varied from 4 × 108 to 5.8 × 108 CFU/g on day 42, the contents of red blood cells, haemoglobin, and white blood cells in the blood of chickens from the experimental group were higher by 6.44%, 3.44%, and 2.11%, respectively, than they were in the control group. Considering the above facts, it can be assumed that the absorption of nutrients in the intestine improved, and metabolic processes were activated due to an increase in the transport abilities of red blood cells.","PeriodicalId":49017,"journal":{"name":"Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice","volume":"1 1","pages":""},"PeriodicalIF":0.4,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68093937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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