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Influence of Agaricus blazei Murill polysaccharides on synthesis, stabilization, acute toxicity and antifungal activity of copper (II) oxide nanoparticles Agaricus blazei Murill 多糖对纳米氧化铜(II)的合成、稳定、急性毒性和抗真菌活性的影响。
IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-21 DOI: 10.1007/s10534-024-00650-w
Álamo Lourenço de Souza, Matheus da Silva Campelo, Gabriel de Sousa Mesquita, Augusto Feynman Dias Nobre, Vitória Maria de Freitas Franco, Antônio César Honorato Barreto, Jeanlex Soares de Sousa, José Eduardo Ribeiro Honório Júnior, Raimunda Sâmia Nogueira Brilhante, Nágila Maria Pontes Silva Ricardo, Sandra de Aguiar Soares, Maria Elenir Nobre Pinho Ribeiro

In general, nanomaterials tend to have better physical, chemical and biological properties than conventional materials. Furthermore, the polysaccharides from Agaricus blazei Murill mushroom have several pharmacological properties, in addition to low cytotoxicity and high biocompatibility. This work sought to merge the properties of CuO nanoparticles and Agaricus blazei Murill polysaccharides through syntheses and coatings with the aim of evaluating their toxicity in adult zebrafish and antifungal activity against C. albicans and C. parapsilosis. The nanoparticles were synthesized using the coprecipitation method and subsequently characterized in terms of their physicochemical properties using spectroscopic and thermoanalytical techniques. Furthermore, their composition was determined by X-Ray Diffraction and their morphology was studied using different microscopic techniques. CuO nanoparticles coated with Agaricus blazei Murill polysaccharides showed smaller particle size. Dispersions of nanoparticles coated with the polysaccharides were found to be more stable than their uncoated counterparts. The nanoparticles also showed antifungal activity against Candida sp. strains, with MIC50 values between 64 and 512 µg mL–1. It was observed that coating the materials with polysaccharides preserved their antifungal properties and reduced acute toxicity against adult zebrafish. Therefore, it is estimated that the CuO nanoparticles coated with Agaricus blazei Murill polysaccharides are innovative nanomaterials with potential for future clinical applications, especially in the topical treatment of candidiasis.

一般来说,纳米材料往往比传统材料具有更好的物理、化学和生物特性。此外,姬松茸多糖除了具有低细胞毒性和高生物相容性外,还具有多种药理特性。这项研究试图通过合成和包覆将氧化铜纳米粒子和姬松茸多糖的特性结合起来,目的是评估它们对成年斑马鱼的毒性以及对白僵菌和副丝状芽孢杆菌的抗真菌活性。纳米颗粒是用共沉淀法合成的,随后用光谱和热分析技术对其理化性质进行了表征。此外,还用 X 射线衍射法测定了它们的组成,并用不同的显微技术研究了它们的形态。用姬松茸 Murill 多糖包覆的氧化铜纳米粒子的粒径较小。涂有多糖的纳米颗粒分散体比未涂多糖的分散体更稳定。纳米颗粒对白色念珠菌菌株也具有抗真菌活性,其 MIC50 值介于 64 至 512 µg mL-1 之间。据观察,在材料上涂覆多糖可保持其抗真菌特性,并降低对成年斑马鱼的急性毒性。因此,估计涂有姬松茸 Murill 多糖的氧化铜纳米颗粒是一种创新的纳米材料,具有未来临床应用的潜力,特别是在局部治疗念珠菌病方面。
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引用次数: 0
Berberine-loaded iron oxide nanoparticles alleviate cuprizone-induced astrocytic reactivity in a rat model of multiple sclerosis 在多发性硬化症大鼠模型中,小檗碱负载的氧化铁纳米粒子可减轻铜绿素诱导的星形胶质细胞反应。
IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-14 DOI: 10.1007/s10534-024-00648-4
Ghadha Ibrahim Fouad, Mostafa Mabrouk, Sara A. M. El-Sayed, Mohamed F. Abdelhameed, Maha Z. Rizk, Hanan H. Beherei

Berberine (BBN) is a naturally occurring alkaloid as a secondary metabolite in many plants and exhibits several benefits including neuroprotective activities. However, data on the neuromodulating potential of nanoformulated BBN are still lacking. In the present study, BBN loaded within iron oxide nanoparticles (BBN-IONP) were prepared and characterized by transmission electron microscopy FTIR, X-ray photoelectron spectroscopy particle-size distribution, zeta potential, and HPLC. The remyelinating neuroprotective potential of BBN-IONP relative to free BBN was evaluated against cuprizone (CPZ)-induced neurotoxicity (rats administered 0.2% CPZ powder (w/w) for five weeks). CPZ rats were treated with either free BBN or IONP-BBN (50 mg/kg/day, orally) for 14 days. Cognitive function was estimated using Y-maze. Biochemically, total antioxidant capacity lipid peroxides and reduced glutathione in the brain tissue, as well as, serum interferon-gamma levels were estimated. Moreover, the genetic expression contents of myelin basic protein Matrix metallopeptidase-9 Tumor necrosis factor-α (TNF-α), and S100β were measured. The histopathological patterns and immunohistochemical assessment of Glial Fibrillary Acidic Protein in both cerebral cortex and hippocampus CA1 regions were investigated. CPZ-rats treated with either free BBN or IONP-BBN demonstrated memory restoring, anti-oxidative, anti-inflammatory, anti-astrocytic, and remyelinating activities. Comparing free BBN with IONP-BBN revealed that the latter altered the neuromodulating activities of BBN, showing superior neuroprotective activities of IONP-BBN relative to BBN. In conclusion, both forms of BBN possess neuroprotective potential. However, the use of IONPs for brain delivery and the safety of these nano-based forms need further investigation.

小檗碱(BBN)是一种天然生物碱,是许多植物的次级代谢产物,具有多种功效,包括神经保护活性。然而,有关纳米配方小檗碱的神经调节潜力的数据仍然缺乏。本研究制备了氧化铁纳米颗粒(BBN-IONP),并通过透射电子显微镜傅立叶变换红外光谱、X射线光电子能谱粒度分布、ZETA电位和高效液相色谱法对其进行了表征。评估了 BBN-IONP 相对于游离 BBN 的再髓鞘化神经保护潜力,以对抗铜绿酸(CPZ)诱导的神经毒性(大鼠服用 0.2% CPZ 粉末(重量比)五周)。CPZ大鼠接受游离BBN或IONP-BBN(50毫克/千克/天,口服)治疗14天。使用Y-迷宫对大鼠的认知功能进行评估。生化方面,对脑组织中的总抗氧化能力脂质过氧化物和还原型谷胱甘肽以及血清干扰素-γ水平进行了评估。此外,还测定了髓鞘碱性蛋白、基质金属肽酶-9、肿瘤坏死因子-α(TNF-α)和 S100β 的基因表达含量。研究了大脑皮层和海马 CA1 区的组织病理学模式和胶质纤维酸性蛋白的免疫组化评估。用游离BBN或IONP-BBN治疗的CPZ大鼠均表现出记忆恢复、抗氧化、抗炎、抗胃囊炎和再髓鞘化等活性。将游离BBN与IONP-BBN进行比较后发现,后者改变了BBN的神经调节活性,显示IONP-BBN的神经保护活性优于BBN。总之,两种形式的BBN都具有神经保护潜力。然而,如何使用 IONPs 给药大脑以及这些纳米形式的安全性还需要进一步研究。
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引用次数: 0
The role of mosses in ‘clean and green’ phytoremediation technology: a review paper 苔藓在 "清洁和绿色 "植物修复技术中的作用:综述论文。
IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-14 DOI: 10.1007/s10534-024-00649-3
Abiy Gezahegn, Eve Bohnett, Siraj Mammo

Phytoremediation, the practice of removing heavy metals from contaminated sites using plants, has emerged as a cost-effective, environmentally friendly green technology to restore damaged ecosystems. Mosses, in particular, demonstrate high phytoremediation potential due to their ability to accumulate heavy metals such as lead, zinc, copper, chromium, cadmium, and iron from contaminated soil and water. This review systematically examines 37 research articles published from 2000 to 2022, focusing on the on the use of mosses for phytoremediation. Moss species, such as Funaria hygrometrica Hedw, Scopelophila cataractae (Mitten) Brotherus, Dicranum scoparium Hedw, Dicranum polysetum Sw. ex anon, Hypnum cupressiforme Hedw, Physcomitrium cyathicarpum Mitt, Barbula constricta Mitt, and Leptodictyum riparium (Hedw.) Warnst. have been identified as ideal candidates for phytoremediation efforts. Specific species of mosses, such as Dicranum species, are noted for their excellent bioaccumulation capabilities of elements like vanadium, manganese, and rubidium, serving as indicators of air pollution. Additionally, Hypnum cupressiforme has proven to be a reliable indicator of sulfur dioxide in natural and anthropogenic sources. This comprehensive review highlights the significant phytoremediation potential of mosses, emphasizing their role as valuable bioaccumulators and indicators of heavy metals and pollutants. The findings highlight the necessity of further research to enhance the application of mosses in environmental management and remediation strategies, ultimately contributing to the development of sustainable and effective solutions for pollution control.

植物修复是指利用植物清除受污染场地中的重金属,它已成为恢复受损生态系统的一种具有成本效益的环保绿色技术。尤其是苔藓植物,由于能够从受污染的土壤和水中积累铅、锌、铜、铬、镉和铁等重金属,因此具有很高的植物修复潜力。本综述系统地研究了 2000 年至 2022 年期间发表的 37 篇研究文章,重点研究了利用苔藓进行植物修复的问题。研究发现,Funaria hygrometrica Hedw、Scopelophila cataractae (Mitten) Brotherus、Dicranum scoparium Hedw、Dicranum polysetum Sw. ex anon、Hypnum cupressiforme Hedw、Physcomitrium cyathicarpum Mitt、Barbula constricta Mitt 和 Leptodictyum riparium (Hedw.) Warnst.等苔藓物种是植物修复的理想候选物种。特定种类的苔藓,如 Dicranum 苔藓,因其对钒、锰和铷等元素具有出色的生物蓄积能力而备受关注,可作为空气污染的指标。此外,Hypnum cupressiforme 被证明是自然和人为来源中二氧化硫的可靠指标。这篇综合综述突出强调了苔藓的巨大植物修复潜力,强调了苔藓作为重金属和污染物的重要生物蓄积剂和指示剂的作用。研究结果强调了进一步研究的必要性,以加强苔藓在环境管理和修复战略中的应用,最终为制定可持续的有效污染控制解决方案做出贡献。
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引用次数: 0
Metallic nanoparticles: a promising novel therapeutic tool against antimicrobial resistance and spread of superbugs 金属纳米粒子:一种有前途的新型治疗工具,可对抗抗菌药耐药性和超级细菌的传播。
IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-24 DOI: 10.1007/s10534-024-00647-5
Naheem Adekilekun Tijani, Joseph Hokello, Emmanuel Eilu, Saheed Adekunle Akinola, Abdullateef Opeyemi Afolabi, Danladi Makeri, Tonny Wotoyitide Lukwago, Irene M. Mutuku, Alex Mwesigwa, Andrew Baguma, Ismail Abiola Adebayo

In recent years, antimicrobial resistance (AMR) has become an alarming threat to global health as notable increase in morbidity and mortality has been ascribed to the emergence of superbugs. The increase in microbial resistance because of harboured or inherited resistomes has been complicated by the lack of new and effective antimicrobial agents, as well as misuse and failure of existing ones. These problems have generated severe and growing public health concern, due to high burden of bacterial infections resulting from scarce financial resources and poor functioning health systems, among others. It is therefore, highly pressing to search for novel and more efficacious alternatives for combating the action of these super bacteria and their infection. The application of metallic nanoparticles (MNPs) with their distinctive physical and chemical attributes appears as promising tools in fighting off these deadly superbugs. The simple, inexpensive and eco-friendly model for enhanced biologically inspired MNPs with exceptional antimicrobial effect and diverse mechanisms of action againsts multiple cell components seems to offer the most promising option and said to have enticed many researchers who now show tremendous interest. This synopsis offers critical discussion on application of MNPs as the foremost intervening strategy to curb the menace posed by the spread of superbugs. As such, this review explores how antimicrobial properties of the metallic nanoparticles which demonstrated considerable efficacy against several multi-drugs resistant bacteria, could be adopted as promising approach in subduing the threat of AMR and harvoc resulting from the spread of superbugs.

近年来,由于超级细菌的出现导致发病率和死亡率显著上升,抗菌药耐药性(AMR)已成为全球健康面临的一个令人担忧的威胁。由于缺乏新的有效抗菌剂,以及现有抗菌剂的滥用和失效,藏匿或遗传的抗菌基因组导致的微生物抗药性增加变得更加复杂。由于财政资源匮乏和卫生系统运作不善等原因造成的细菌感染负担沉重,这些问题引发了严重且日益严重的公共卫生问题。因此,寻找新型、更有效的替代品来对抗这些超级细菌的作用及其感染已迫在眉睫。金属纳米粒子(MNPs)具有独特的物理和化学属性,其应用似乎有望成为抵御这些致命超级细菌的工具。受生物启发的 MNPs 具有卓越的抗菌效果和针对多种细胞成分的不同作用机制,这种简单、廉价、环保的增强型 MNPs 模型似乎提供了最有前途的选择,据说吸引了许多研究人员,他们现在对此表现出了极大的兴趣。本综述对应用 MNPs 作为遏制超级细菌传播威胁的首要干预策略进行了深入探讨。因此,本综述探讨了金属纳米粒子的抗菌特性(该特性对多种耐药细菌具有显著疗效)可如何作为一种有前途的方法来抑制因超级细菌传播而导致的 AMR 和 Harvoc 威胁。
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引用次数: 0
Copper biomarkers and their relationship with dyslipidemia in women with obesity 肥胖妇女体内的铜生物标志物及其与血脂异常的关系。
IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-16 DOI: 10.1007/s10534-024-00641-x
Lyandra Dias da Silva, Nilmara Cunha da Silva, Bianca Mickaela Santos Chaves, Loanne Rocha Santos, Thayanne Gabryelle Visgueira de Sousa, Emyle Horrana Serafim de Oliveira, Tamires da Cunha Soares, Stéfany Rodrigues de Sousa Melo, Gilberto Simeone Henriques, Francisco Erasmo de Oliveira, Juliana Soares Severo, Jorddam Almondes Martins, Esmeralda Maria Lustosa Barros, Mayara Storel Beserra de Moura, Dilina do Nascimento Marreiro

Adipose tissue dysfunction influences the development of dyslipidemias associated with obesity, however, the mechanisms are still unclear. In this sense, the literature highlights the role of copper in cholesterol synthesis, contributing to the increase in concentrations of this lipid fraction and consequently to the manifestation of dyslipidemia. The objective of the study was to investigate the relationship between copper parameters and lipid profile markers in women with obesity. This is a cross-sectional study involving women aged 20 to 50 years divided into a case group (BMI ≥ 35 kg/m2) and a control group (BMI between 18.5 and 24.9 kg/m2). Copper concentrations in plasma and erythrocytes were determined by inductively coupled plasma optical emission spectrophotometry and ceruloplasmin activity by spectrophotometry. The lipid fractions were analyzed according to the enzymatic colorimetric method, using an automatic biochemical analyzer. Participants with obesity had elevated concentrations of copper in plasma and reduced concentrations in erythrocytes compared to the control group, but there was no significant difference in ceruloplasmin activity between the groups. The research does not identify a correlation between copper parameters and serum concentrations of lipid fractions, which does not allow inferring the role of copper in the manifestation of dyslipidemia in obesity.

脂肪组织功能障碍会影响与肥胖有关的血脂异常的发生,但其机制仍不清楚。从这个意义上讲,文献强调了铜在胆固醇合成中的作用,它有助于增加这种脂质部分的浓度,从而导致血脂异常的表现。本研究的目的是调查肥胖女性体内铜参数与血脂指标之间的关系。这是一项横断面研究,涉及 20 至 50 岁的女性,分为病例组(体重指数≥ 35 kg/m2)和对照组(体重指数介于 18.5 至 24.9 kg/m2)。采用电感耦合等离子体光发射分光光度法测定血浆和红细胞中的铜浓度,采用分光光度法测定脑磷脂酶活性。脂质组分则使用自动生化分析仪,按照酶比色法进行分析。与对照组相比,肥胖症参与者血浆中的铜浓度升高,红细胞中的铜浓度降低,但两组之间的脑磷脂酶活性没有显著差异。这项研究没有发现铜参数与血清中脂质组分浓度之间的相关性,因此无法推断铜在肥胖症患者血脂异常表现中的作用。
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引用次数: 0
Effect of co-exposure to multiple metals (Pb, Cd, Cr, Hg, Fe, Mn and Ni) and metalloid (As) on liver function in Swiss albino mice 共同暴露于多种金属(铅、镉、铬、汞、铁、锰和镍)和类金属(砷)对瑞士白化小鼠肝功能的影响
IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-16 DOI: 10.1007/s10534-024-00643-9
Damini Singh, Priyanka Bist, Sangeeta Choudhary

The study examined the cumulative toxic effect of multiple elements, As, Pb, Cd, Cr, Hg, Fe, Mn and Ni on the liver function and their association with inflammation and apoptosis. To explore the health consequence of simultaneous exposure to multiple metals and metalloid, male and female Swiss Albino mice were randomly divided into 14 groups and subjected to different doses [MPL (maximum permissible limit), 1×, 5×, 10×, 50× or 100×] of metal(loid)s mixture via drinking water for 8 weeks. Data showed that combined effect of multiple elements impaired the liver function. This was associated with significant decrease in the antioxidant enzymes and the elevation in lipid peroxidation for high exposure dose of 50× and 100× (p < 0.05). The metal(loid)s mixture exposure led to significant increase (p < 0.05) in cytokines, TNF-α, IL-6 and effector caspases (3 and 6) in exposure groups above 10× dose. Histopathological observation also revealed significant damage in the hepatic tissue on exposure to high dose. Dose dependent accumulation of respective elements (As, Cd, Cr, and Pb) in the liver was observed in each of the exposure groups. However, similar dose related increment was not observed for essential metals such as Ni, Fe and Mn. Differential accumulation of metals in the liver may be attributed to the effect of co-contaminant exposure, which could affect the divalent cation absorption due to antagonism and competitive transport process. Overall findings in this study manifest the complexity of possible joint effect of co-exposure to multiple metals and metalloid on the liver function.

该研究考察了多种元素(砷、铅、镉、铬、汞、铁、锰和镍)对肝功能的累积毒性效应及其与炎症和细胞凋亡的关系。为探讨同时暴露于多种金属和类金属对健康的影响,研究人员将雌雄瑞士白化小鼠随机分为 14 组,分别通过饮水摄入不同剂量[MPL(最大允许限)、1×、5×、10×、50×或 100×]的金属(类)混合物,连续摄入 8 周。数据显示,多种元素的综合作用损害了肝功能。在 50× 和 100× 的高暴露剂量下,抗氧化酶明显降低,脂质过氧化物增加(p
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引用次数: 0
A new spectrophotometric method for measuring ceruloplasmin ferroxidase activity: an innovative approach 测量脑磷脂铁氧化酶活性的新分光光度法:一种创新方法。
IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-14 DOI: 10.1007/s10534-024-00635-9
Mahmoud Hussein Hadwan, Ahed Kamil Rahi, Esraa Rafied Abass, Asad M. Hadwan, Rawa M. Mohammed, Abdulsamie Hassan Alta’ee, Abdul Razzaq Alsalman, Muntadher M. Hadwan, Zainab Abbas Al-Talebi

Ferroxidases are enzymes that participate in the iron metabolism of different organisms. They catalyze the oxidation of ferrous iron, Fe2⁺, into ferric iron, Fe3⁺, which is essential in iron homeostasis and physiological functioning. The present study describes a novel spectrophotometric method of serum ceruloplasmin ferroxidase activity. This method is easy to perform; it is also sensitive, specific, and rapid. In this method, ferrous ions are used as a substrate for the enzyme, with either salicylic acid or sulfosalicylic acid being taken as a chromogenic compound. These chromogens easily form a colored complex with ferric ions but are not formed with ferrous ions. In the enzymatic reaction, the ceruloplasmin ferroxidase enzyme catalyzes the oxidation of ferrous to ferric ions. The resulting increase in ferric ion concentration is then measured spectrophotometrically, following the formation of the colored complex. The complex formed has maximum absorbance at 540 nm in the case of salicylic acid and 490 nm in the case of sulfosalicylic acid. Comparatively, it was tested against the standard method to ascertain the new method’s effectuality and reliability for assaying ferroxidase activity. The determined correlation coefficient amounted to 0.99, showing a strong correlation between the results obtained by the two methods. This new spectrophotometric technique offers a simplified, sensitive, specific, and fast means of estimating ferroxidase activity. It avoids using concentrated strong acids in the procedure and correlates excellently with the standard technique. This sets up a potential alternative for accurately determining ferroxidase activity in biological samples.

Graphical abstract

铁氧化酶是一种参与不同生物体铁代谢的酶。它们催化亚铁(Fe2⁺)氧化成铁素体(Fe3⁺),而铁素体是铁平衡和生理功能所必需的。本研究介绍了一种新型分光光度法测定血清脑磷脂铁氧化酶活性。该方法操作简便,灵敏、特异、快速。在该方法中,亚铁离子被用作酶的底物,水杨酸或磺基水杨酸被用作发色剂。这些致色剂很容易与铁离子形成有色复合物,但不会与亚铁离子形成有色复合物。在酶促反应中,脑磷脂铁氧化酶催化亚铁氧化成铁离子。在有色复合物形成后,用分光光度法测量铁离子浓度的增加。水杨酸形成的复合物在 540 纳米波长处有最大吸光度,磺基水杨酸则在 490 纳米波长处有最大吸光度。为了确定新方法在测定铁氧化酶活性方面的有效性和可靠性,我们将其与标准方法进行了比较试验。测定的相关系数为 0.99,表明两种方法得出的结果之间具有很强的相关性。这种新的分光光度法提供了一种简化、灵敏、特异和快速的估算铁氧化酶活性的方法。它避免了在操作过程中使用浓强酸,并与标准技术有很好的相关性。这为准确测定生物样本中的铁氧化酶活性提供了一种潜在的替代方法。
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引用次数: 0
The arylbipyridine platinum (II) complex increases the level of ROS and induces lipid peroxidation in glioblastoma cells 芳基联吡啶铂(II)复合物会增加胶质母细胞瘤细胞中的 ROS 水平并诱导脂质过氧化。
IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-14 DOI: 10.1007/s10534-024-00646-6
Maria D. Tokhtueva, Vsevolod V. Melekhin, Vladislav M. Abramov, Alexander I. Ponomarev, Anna V. Prokofyeva, Kirill V. Grzhegorzhevskii, Anastasia V. Paramonova, Oleg G. Makeev, Oleg S. Eltsov

Here we present the biological properties of the arylbipyridine platinum (II) complex (arylbipy-Pt) and describe the potential mechanism of its antitumor action which differs from that of the well-known cisplatin. Leading to the oxidative stress and lipid peroxidation, the arylbipyridine platinum (II) complex showcases the significant cytotoxicity against the glioblastoma cells as shown by the MTT test. Using the 5-ethyl-2-deoxyuridine we study the proliferative activity of glioblastoma cells to affirm that arylbipyridine platinum (II) complex does not impede cell division or DNA replication. Staining by the MitoCLox dye and 2′,7′-dichlorodihydrofluorescein diacetate demonstrates that the glioblastoma cells treated with arylbipy-Pt exhibit a strong increase of the lipid peroxidation and the stimulation of the reactive oxygen species formation. The hypothesis that arylbipy-Pt promotes oxidative death of tumor cells is confirmed by control experiments using N-acetyl-L-cysteine as an antioxidant. Further evidence for the oxidative mechanism of action is provided by real-time PCR, which shows high expression levels for genes associated with the heat shock proteins HSP27 and HSP70, which can be used as markers of tumor cell ferroptosis. To elucidate the chemical nature of the arylbipy-Pt complex activity, we perform 195Pt NMR spectroscopy and cyclic voltammetry measurements under biologically relevant conditions. The results obtained clearly indicate the structural transformation of the arylbipy-Pt complex in the DMSO-saline mixture, which is crucial for its further antitumor activity via the oxidative pathway. The found correlation between the molecular structure of arylbipy-Pt and its effect on the tumor cell cycle paves the way for the rational design of Pt complexes possessing the alternative mechanism of antitumor activity as compared to DNA intercalation, providing possible solutions to the major problems such as toxicity and drug resistance.

在此,我们介绍了芳基联吡啶铂(II)复合物(rylbipy-Pt)的生物学特性,并描述了其与众所周知的顺铂不同的潜在抗肿瘤作用机制。芳基联吡啶铂(II)复合物会导致氧化应激和脂质过氧化,MTT 试验表明,它对胶质母细胞瘤细胞具有显著的细胞毒性。我们使用 5-乙基-2-脱氧尿苷研究了胶质母细胞瘤细胞的增殖活性,以确定芳基联吡啶铂(II)复合物不会阻碍细胞分裂或 DNA 复制。用 MitoCLox 染料和 2',7'-二氯二氢荧光素二乙酸酯染色表明,用 arylbipy-Pt 处理过的胶质母细胞瘤细胞的脂质过氧化反应和活性氧形成受到刺激。使用 N-乙酰-L-半胱氨酸作为抗氧化剂进行的对照实验证实了 arylbipy-Pt 促进肿瘤细胞氧化死亡的假设。实时聚合酶链式反应(real-time PCR)提供了氧化作用机制的进一步证据,它显示了与热休克蛋白 HSP27 和 HSP70 相关的基因的高表达水平,这些基因可用作肿瘤细胞铁变态反应的标志物。为了阐明 arylbipy-Pt 复合物活性的化学本质,我们在生物相关条件下进行了 195Pt NMR 光谱和循环伏安法测量。研究结果清楚地表明,芳基联吡-铂复合物在二甲基亚砜-盐水混合物中发生了结构转变,这对其通过氧化途径进一步发挥抗肿瘤活性至关重要。所发现的 arylbipy-Pt 分子结构与其对肿瘤细胞周期的影响之间的相关性,为合理设计铂复合物铺平了道路,这种铂复合物与 DNA 插层相比,具有另一种抗肿瘤活性机制,为毒性和耐药性等主要问题提供了可能的解决方案。
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引用次数: 0
Copper biomarkers and their relationship with parameters of insulin resistance in obese women 肥胖妇女体内的铜生物标志物及其与胰岛素抵抗参数的关系。
IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-11 DOI: 10.1007/s10534-024-00645-7
Nilmara Cunha da Silva, Lyandra Dias da Silva, Bianca Mickaela Santos Chaves, Emyle Horrana Serafim de Oliveira, Esmeralda Maria Lustosa Barros, Francisco Erasmo de Oliveira, Gilberto Simeone Henriques, Irislene Costa Pereira, Juliana Soares Severo, Loanne Rocha dos Santos, Mayara Storel Beserra de Moura, Tamires da Cunha Soares, Thayanne Gabryelle Visgueira de Sousa, Stéfany Rodrigues de Sousa Melo, Dilina do Nascimento Marreiro

Some studies have demonstrated the involvement of high concentrations of copper in the manifestation of insulin resistance in individuals with obesity. The objective of this study was to evaluate the copper nutritional status and its relationship with parameters of glycemic control in women with obesity. An observational case–control clinical study involving 203 women aged between 20 and 50 years, divided into two groups: obese (n = 84) and eutrophic (n = 119). Body weight, height and waist, hip and neck circumferences, dietary copper intake, copper biomarkers, determine ceruloplasmin activity and glycemic control parameters were measured. It was observed that women with obesity had higher copper concentrations in plasma and lower concentrations in erythrocytes when compared to the control group. Analysis of glycemic control parameters revealed a statistically significant difference in fasting blood glucose (p < 0.05) between groups. The study identified a significant positive correlation between plasma copper and fasting insulin values and the Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) index (p < 0.05). The results of this study demonstrate that obese women have high copper concentrations in plasma and lower concentrations in erythrocytes. Furthermore, the significant positive correlation between plasma copper and fasting insulin and HOMA-IR index suggests the influence of this mineral on glycemic control parameters in obese women.

一些研究表明,高浓度铜参与了肥胖症患者胰岛素抵抗的表现。本研究的目的是评估肥胖女性的铜营养状况及其与血糖控制参数之间的关系。这项病例对照临床观察研究涉及 203 名 20 至 50 岁的女性,分为两组:肥胖组(84 人)和营养不良组(119 人)。研究测量了体重、身高、腰围、臀围和颈围、膳食铜摄入量、铜生物标志物、脑磷脂酶活性和血糖控制参数。结果发现,与对照组相比,肥胖妇女血浆中的铜浓度较高,而红细胞中的铜浓度较低。血糖控制参数分析表明,肥胖妇女的空腹血糖与对照组相比有显著的统计学差异(p
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引用次数: 0
Synthesis of metallic nanoparticles using biometabolites: mechanisms and applications 利用生物代谢物合成金属纳米颗粒:机制与应用。
IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-08 DOI: 10.1007/s10534-024-00642-w
Chinmayee Acharya, Sonam Mishra, Sandeep Kumar Chaurasia, Bishnu Kumar Pandey, Ravindra Dhar, Jitendra Kumar Pandey

Bio-metabolites have played a crucial role in the recent green synthesis of nanoparticles, resulting in more versatile, safer, and effective nanoparticles. Various primary and secondary metabolites, such as proteins, carbohydrates, lipids, nucleic acids, enzymes, vitamins, organic acids, alkaloids, flavonoids, and terpenes, have demonstrated strong metal reduction and stabilization properties that can be utilized to synthesize nanomaterials and influence their characters. While physical and chemical methods were previously used to synthesize these nanomaterials, their drawbacks, including high energy consumption, elevated cost, lower yield, and the use of toxic chemicals, have led to a shift towards eco-friendly, rapid, and efficient alternatives. Biomolecules act as reducing agents through deprotonation, nucleophilic reactions, transesterification reactions, ligand binding, and chelation mechanisms, which help sequester metal ions into stable metal nanoparticles (NPs). Engineered NPs have potential applications in various fields due to their optical, electronic, and magnetic properties, offering improved performance compared to bulkier counterparts. NPs can be used in medicine, food and agriculture, chemical catalysts, energy harvesting, electronics, etc. This review provides an overview of the role of primary and secondary metabolites in creating effective nanostructures and their potential applications.

生物代谢物在近年来纳米粒子的绿色合成中发挥了至关重要的作用,从而产生了用途更广、更安全、更有效的纳米粒子。各种初级和次级代谢物,如蛋白质、碳水化合物、脂类、核酸、酶、维生素、有机酸、生物碱、黄酮类化合物和萜类化合物,都具有很强的金属还原和稳定特性,可用于合成纳米材料并影响其特性。以前人们使用物理和化学方法合成这些纳米材料,但这些方法存在能耗高、成本高、产量低和使用有毒化学品等缺点,因此人们开始转向环保、快速和高效的替代方法。生物大分子通过去质子化、亲核反应、酯化反应、配体结合和螯合机制充当还原剂,有助于将金属离子封存到稳定的金属纳米粒子(NPs)中。由于具有光学、电子和磁性等特性,工程纳米粒子在各个领域都有潜在的应用前景。NPs 可用于医药、食品和农业、化学催化剂、能量收集、电子等领域。本综述概述了初级和次级代谢物在创建有效纳米结构及其潜在应用中的作用。
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引用次数: 0
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Biometals
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