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Willingness to pay for residential property-based and community-based tick control methods in Lyme disease-endemic areas of the Upper Midwest, United States 在美国上中西部莱姆病流行地区,居民愿意支付基于住宅物业和基于社区的蜱虫控制方法
IF 3.4 2区 医学 Q2 INFECTIOUS DISEASES Pub Date : 2025-08-06 DOI: 10.1016/j.ttbdis.2025.102531
Elizabeth K. Schiffman , Jenna Bjork , Daniel Phaneuf , Alyssa Beck , Erik Foster , Jean I. Tsao , Rebecca Osborn , Rebecca Eisen , Susan Paskewitz , Sarah A. Hook , Alison F. Hinckley
Ticks and tickborne diseases are of increasing concern in the United States, and the burden is high in certain focal areas. While the acceptability of various tick control and disease prevention methods has been studied, the public’s willingness to pay for environmental interventions at the individual or community level is less well described. Using data collected as part of a larger survey, we performed an additional analysis of residents of Lyme disease-endemic counties of Michigan, Minnesota, and Wisconsin to assess their willingness to support and pay annually for various methods of property-based tick control and examined demographic characteristics that might influence willingness to pay. Seventy-nine percent of respondents were willing to perform some form of tick control on their property, with most preferring self-application. Landscaping and natural pesticide application were the most popular options, with people willing to pay an estimated $78 and $61 annually, respectively. High income, a high perceived prevalence of disease, and a high perceived likelihood of disease were all associated with a willingness to pay more. When asked about a community control option, 97 % of respondents indicated interest, with respondents being willing to pay $52/year for a community-based program regardless of household characteristics. These results suggest a moderate demand in the Upper Midwest for tick control efforts at both the individual property level and for local, publicly funded, community-based programs. These findings provide a starting point for assessing community characteristics, cost structure, environmental attributes, and efficacy needed to generate net benefits for community-based tick control programs.
蜱和蜱传疾病在美国日益受到关注,在某些重点地区负担很高。虽然对各种蜱虫控制和疾病预防方法的可接受性进行了研究,但公众在个人或社区一级为环境干预措施付费的意愿却没有得到很好的描述。使用作为更大调查的一部分收集的数据,我们对密歇根州、明尼苏达州和威斯康星州莱姆病流行县的居民进行了额外的分析,以评估他们支持和每年支付各种基于财产的蜱虫控制方法的意愿,并检查可能影响支付意愿的人口统计学特征。79%的受访者愿意对他们的财产进行某种形式的蜱虫控制,大多数人更喜欢自我应用。园林绿化和天然农药是最受欢迎的选择,人们愿意每年分别支付78美元和61美元。高收入、高疾病流行率和高患病可能性都与支付更多费用的意愿相关。当被问及社区控制选项时,97%的受访者表示有兴趣,无论家庭特征如何,受访者都愿意每年支付52美元用于社区控制方案。这些结果表明,在中西部上游地区,无论是在个人财产层面,还是在地方、公共资助的、以社区为基础的项目上,蜱虫控制工作的需求都是适度的。这些发现为评估社区特征、成本结构、环境属性和为社区蜱虫控制项目产生净效益所需的有效性提供了一个起点。
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引用次数: 0
Spatial distribution and clustering of medically important tick species in Illinois: Implications for tick-borne disease risk 伊利诺伊州医学上重要蜱类的空间分布和聚集:对蜱传疾病风险的影响
IF 3.4 2区 医学 Q2 INFECTIOUS DISEASES Pub Date : 2025-08-06 DOI: 10.1016/j.ttbdis.2025.102533
Abrar Hussain , Csaba Varga , Brian F. Allan , Nohra Mateus-Pinilla , Rebecca L. Smith
The geographic range of medically significant tick species has expanded across the United States, with Illinois experiencing increased tick populations and incidence of tick-borne diseases (TBDs) in recent decades. This study investigated the spatial distribution of three tick species: Amblyomma americanum (lone star tick), Dermacentor variabilis (American dog tick), and Ixodes scapularis (blacklegged tick) in Illinois, using data collected through active surveillance from 2018 to 2022. A total of 476 records of 1414 ticks were analyzed. Spatial analytical techniques, including species distribution mapping, Inverse Distance Weighted (IDW) interpolation, Hot Spot (Getis-Ord Gi*), and Cluster and Outlier (Anselin Local Moran’s I) analyses, were applied to identify distribution patterns and statistically significant local clusters. The species distribution mapping results revealed distinct geographic patterns: A. americanum was most prevalent in southern, D. variabilis in central and southern, and I. scapularis in central and northeastern Illinois regions, respectively. With agreement between Hot Spot and Cluster-outlier analysis, counties with significantly high tick prevalence were identified, including 10 counties for A. americanum in southern Illinois, four counties for D. variabilis in central and southern Illinois, and seven counties for I. scapularis in central and northeastern Illinois. The study results correspond with incidence of TBDs reported to the Illinois Department of Public Health (IDPH), including ehrlichiosis, Rocky Mountain spotted fever, Lyme disease, and anaplasmosis. These findings provide insights into the geographic variability of tick distributions, emphasizing the importance of targeted public health strategies and tick control efforts to mitigate the burden of TBDs in Illinois.
在美国,具有重要医学意义的蜱虫物种的地理范围已经扩大,近几十年来,伊利诺伊州的蜱虫种群和蜱媒疾病(tbd)的发病率都在增加。本研究利用2018年至2022年通过主动监测收集的数据,调查了伊利诺伊州三种蜱的空间分布:Amblyomma americanum(孤星蜱),Dermacentor variabilis(美国狗蜱)和Ixodes scapularis(黑腿蜱)。共分析了1414只蜱的476条记录。利用物种分布图、IDW插值、热点分析(Getis-Ord Gi*)和聚类与离群分析(Anselin Local Moran’s I)等空间分析技术,对物种分布格局和具有统计学意义的局部聚类进行了识别。物种分布图显示出明显的地理分布格局:美洲蠓分布于伊利诺伊州南部,变异蠓分布于伊利诺伊州中部和南部,肩胛骨蠓分布于伊利诺伊州中部和东北部。热点分析与聚类异常值分析结果一致,确定了蜱虫流行率较高的县,其中南部有10个县为美洲伊蚊,中部和南部有4个县为变异伊蚊,中部和东北部有7个县为肩背伊蚊。研究结果与伊利诺斯州公共卫生部(IDPH)报告的tds发病率一致,包括埃利希体病、落基山斑疹热、莱姆病和无形体病。这些发现为蜱分布的地理变异性提供了见解,强调了有针对性的公共卫生战略和蜱控制工作对减轻伊利诺伊州tbd负担的重要性。
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引用次数: 0
Association between tick-bite history and safety of gelatin-containing vaccines: Analysis of a large database of the United States 蜱叮咬史与含明胶疫苗安全性之间的关系:对美国大型数据库的分析。
IF 3.4 2区 医学 Q2 INFECTIOUS DISEASES Pub Date : 2025-08-05 DOI: 10.1016/j.ttbdis.2025.102532
Chia-Yu Chiu , Andrés F Henao-Martínez , Nelson Iván Agudelo Higuita
Alpha-gal syndrome (AGS) is an allergic reaction caused by sensitization to alpha-gal, a sugar molecule in gelatin-containing vaccines. Tick bites can induce alpha-gal IgE sensitization, as ticks inject alpha-gal through their saliva. However, there is no real-world data on the anaphylaxis rates associated with gelatin-containing vaccines in patients with tick bite history. We utilized TriNetX, a research network database, to analyze data in November 2024. The history of tick bites (i.e., the presence of a tick-bite history) was categorized into three groups: ehrlichiosis, tularemia, and bites from nonvenomous arthropods. Vaccines were classified into two categories: (1) gelatin-containing vaccines (live intranasal influenza [FluMist], varicella, measles/mumps/rubella [MMR], oral typhoid, rabies [RabAvert], and yellow fever vaccines [YF-VAX]) and (2) gelatin-free vaccines. Anaphylactic episodes were identified in patients with a history of tick bites who developed anaphylaxis within one day following vaccination. The anaphylaxis rates after receiving gelatin-containing vaccines were 0.06 %, 0.7 %, and 0.2 % in patients with ehrlichiosis, tularemia, and nonvenomous arthropod bites, respectively. In contrast, the anaphylaxis rates after receiving gelatin-free vaccines were 0.04 %, 0.0 %, and 0.1 % in patients with ehrlichiosis, tularemia, and nonvenomous arthropod bites, respectively. Among the gelatin-containing vaccines, live intranasal influenza and varicella vaccines posed a higher rate of anaphylaxis compared to other gelatin-containing vaccines. While tick bites are associated with AGS, a history of tick bites is not a contraindication for most gelatin-containing vaccines, including MMR, oral typhoid, rabies, and yellow fever vaccines. Further investigations are warranted to evaluate the safety of intranasal influenza and varicella vaccines in this population.
α -半乳糖综合征(AGS)是对含明胶疫苗中的糖分子α -半乳糖致敏引起的过敏反应。蜱虫叮咬可以诱导α -半乳糖IgE致敏,因为蜱虫通过唾液注入α -半乳糖。然而,在有蜱虫叮咬史的患者中,没有关于含明胶疫苗的过敏反应率的真实数据。我们利用研究网络数据库TriNetX分析了2024年11月的数据。蜱叮咬史(即存在蜱叮咬史)分为三组:埃立体病、土拉菌病和无毒节肢动物叮咬。疫苗分为两类:(1)含明胶疫苗(鼻内活流感疫苗[fluumist]、水痘、麻疹/腮腺炎/风疹疫苗[MMR]、口服伤寒、狂犬病疫苗[RabAvert]和黄热病疫苗[YF-VAX])和(2)无明胶疫苗。有蜱虫叮咬史的患者在接种疫苗后一天内出现过敏反应。埃立克体病、兔吸虫病和无毒节肢动物咬伤患者接种含明胶疫苗后的过敏反应率分别为0.06%、0.7%和0.2%。相比之下,接受无明胶疫苗后的过敏反应率在埃利希体病、土拉菌病和无毒节肢动物咬伤患者中分别为0.04%、0.0%和0.1%。在含明胶疫苗中,与其他含明胶疫苗相比,鼻内流感和水痘活疫苗具有更高的过敏反应率。虽然蜱叮咬与AGS有关,但蜱叮咬史并不是大多数含明胶疫苗的禁忌症,包括MMR、口服伤寒、狂犬病和黄热病疫苗。需要进一步的调查来评估鼻内流感和水痘疫苗在这一人群中的安全性。
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引用次数: 0
An update on Borrelia burgdorferi s.l. prevalence and hazard in ticks at recreational areas in England and Wales between 2021 and 2023 2021年至2023年期间英格兰和威尔士休闲区蜱虫中的伯氏疏螺旋体流行率和危害的最新情况
IF 3.4 2区 医学 Q2 INFECTIOUS DISEASES Pub Date : 2025-07-28 DOI: 10.1016/j.ttbdis.2025.102523
Sara L. Gandy , Faye Brown , Jonathan Yardley , Nicola Jones , Anthony Abbott , Sarah Biddlecombe , Colin Johnston , Kayleigh Hansford , Andrew Nelson , Christopher Williams , Jolyon M. Medlock
Estimating tick-borne disease hazard in highly visited natural landscapes is essential for assessing public health risks. Lyme disease, caused by bacteria of the Borrelia burgdorferi sensu lato (s.l.) complex and transmitted by the tick Ixodes ricinus in Europe, is the most prevalent vector-borne disease. This study aimed to measure Lyme disease hazard across a range of recreational areas, building on previous research. From 2021 to 2023, 84 sites within 36 recreational areas across England and Wales were sampled. The density of questing I. ricinus nymphs was significantly higher in woodlands than in grasslands and significantly increased in the presence of deer. Tick density was significantly higher in the north and south of England compared to central England. The overall prevalence of B. burgdorferi s.l. in I. ricinus nymphs was 5.8 % [95 % CI: 5.1-6.6 %] (228/3914), with site-level prevalences ranging from 0 % to 30.4 %. The dominant genospecies was B. garinii (39 % of positive samples), followed by B. valaisiana (21.9 %), B. afzelii (15.4 %) and B. burgdorferi s.s. (3.1 %), which sets England and Wales apart from other European countries where B. afzelii typically dominates. The density of nymphs infected with B. burgdorferi s.l., which represents Lyme disease hazard, was significantly higher in the north and south of England compared to central England and Wales. Notably, the highest densities of infected nymphs were identified in Kielder Forest, Yorkshire Dales, Exmoor, Blackdown Hills and South Devon. We did not find any significant association between the density of infected nymphs and the presence of deer at the survey location.
在游客较多的自然景观中估计蜱传疾病危害对于评估公共卫生风险至关重要。莱姆病由伯氏疏螺旋体(Borrelia burgdorferi)复合菌引起,由欧洲的蓖麻蜱(Ixodes ricinus)传播,是最普遍的媒介传播疾病。这项研究旨在测量莱姆病在一系列娱乐场所的危害,以先前的研究为基础。从2021年到2023年,对英格兰和威尔士36个休闲区的84个地点进行了采样。在林地中,猎取蓖麻螨若虫的密度显著高于草地;在有鹿的情况下,猎取蓖麻螨若虫的密度显著增加。与英格兰中部相比,英格兰北部和南部的蜱虫密度明显更高。布氏疏螺旋体在蓖麻螨蛹中的总体流行率为5.8% [95% CI: 5.1 ~ 6.6%](228/3914),站点水平的流行率为0% ~ 30.4%。显性基因种是b garinii阳性样品(39%),紧随其后的是b valaisiana (21.9%), b . afzelii(15.4%)和b burgdorferi砂岩(3.1%),使英格兰和威尔士有别于其他欧洲国家b afzelii通常占主导地位。感染代表莱姆病危险的伯氏疏螺旋体的若虫密度在英格兰北部和南部明显高于英格兰中部和威尔士。值得注意的是,受感染的若虫密度最高的地区是基尔德森林、约克郡山谷、埃克斯穆尔、布莱克当山和南德文郡。在调查地点,我们没有发现感染若虫的密度与鹿的存在之间有任何显著的关联。
{"title":"An update on Borrelia burgdorferi s.l. prevalence and hazard in ticks at recreational areas in England and Wales between 2021 and 2023","authors":"Sara L. Gandy ,&nbsp;Faye Brown ,&nbsp;Jonathan Yardley ,&nbsp;Nicola Jones ,&nbsp;Anthony Abbott ,&nbsp;Sarah Biddlecombe ,&nbsp;Colin Johnston ,&nbsp;Kayleigh Hansford ,&nbsp;Andrew Nelson ,&nbsp;Christopher Williams ,&nbsp;Jolyon M. Medlock","doi":"10.1016/j.ttbdis.2025.102523","DOIUrl":"10.1016/j.ttbdis.2025.102523","url":null,"abstract":"<div><div>Estimating tick-borne disease hazard in highly visited natural landscapes is essential for assessing public health risks. Lyme disease, caused by bacteria of the <em>Borrelia burgdorferi</em> sensu lato (s.l.) complex and transmitted by the tick <em>Ixodes ricinus</em> in Europe, is the most prevalent vector-borne disease. This study aimed to measure Lyme disease hazard across a range of recreational areas, building on previous research. From 2021 to 2023, 84 sites within 36 recreational areas across England and Wales were sampled. The density of questing <em>I. ricinus</em> nymphs was significantly higher in woodlands than in grasslands and significantly increased in the presence of deer. Tick density was significantly higher in the north and south of England compared to central England. The overall prevalence of <em>B. burgdorferi</em> s.l. in <em>I. ricinus</em> nymphs was 5.8 % [95 % CI: 5.1-6.6 %] (228/3914), with site-level prevalences ranging from 0 % to 30.4 %. The dominant genospecies was <em>B. garinii</em> (39 % of positive samples), followed by <em>B. valaisiana</em> (21.9 %), <em>B. afzelii</em> (15.4 %) and <em>B. burgdorferi</em> s.s. (3.1 %), which sets England and Wales apart from other European countries where <em>B. afzelii</em> typically dominates. The density of nymphs infected with <em>B. burgdorferi</em> s.l., which represents Lyme disease hazard, was significantly higher in the north and south of England compared to central England and Wales. Notably, the highest densities of infected nymphs were identified in Kielder Forest, Yorkshire Dales, Exmoor, Blackdown Hills and South Devon. We did not find any significant association between the density of infected nymphs and the presence of deer at the survey location.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 5","pages":"Article 102523"},"PeriodicalIF":3.4,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144721583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A comparison of reverse line blot hybridization and IDEXX SNAP 4Dx® test in detecting canine anaplasmosis/ehrlichiosis in Trinidad 逆行杂交与IDEXX SNAP 4Dx®检测特立尼达犬无形体病/埃利希体病的比较
IF 3.1 2区 医学 Q2 INFECTIOUS DISEASES Pub Date : 2025-07-24 DOI: 10.1016/j.ttbdis.2025.102517
Ansarah Hosein , Candice Sant , Indira Pargass , Gowrie Lalla , Fatima Mohammed , Karla Georges
Ehrlichia canis and Anaplasma platys are known to be among the most common tick-borne hemopathogens of dogs in Trinidad that are transmitted by ixodid ticks of the Rhipicephalus sanguineus sensu lato species complex. These pathogens are commonly diagnosed in Trinidad based on clinical signs, laboratory tests and response to treatment. However, as these hemopathogens are often not observed on microscopic examination of blood smears, alternative methods to provide a definitive diagnosis are warranted. The IDEXX SNAP 4Dx® test is used frequently by veterinarians in Trinidad to determine exposure status and inform decisions on whether a canine patient should be treated. This study investigated the use of the IDEXX SNAP 4Dx® test and polymerase chain reaction (PCR) followed by a reverse line blot hybridization (RLB) assay on samples obtained from 231 dogs in order to determine if there was an acceptable level of agreement between the two tests. The study showed poor agreement between the IDEXX SNAP 4Dx® test and RLB.
众所周知,犬埃利希体和鸭无原体是特立尼达最常见的蜱传犬血病原体,它们是由吸血鼻头蜱的蜱虫物种复合体传播的。在特立尼达,通常根据临床症状、实验室检查和对治疗的反应来诊断这些病原体。然而,由于这些病原体在血液涂片的显微镜检查中往往没有观察到,因此有必要采用其他方法来提供明确的诊断。特立尼达的兽医经常使用IDEXX SNAP 4Dx®测试来确定犬类患者的暴露状况,并决定是否应该对其进行治疗。本研究对从231只狗身上获得的样本进行了IDEXX SNAP 4Dx®检测和聚合酶链反应(PCR)以及反向行杂交(RLB)检测,以确定这两种检测之间是否存在可接受的一致性水平。该研究显示IDEXX SNAP 4Dx®测试与RLB之间的一致性较差。
{"title":"A comparison of reverse line blot hybridization and IDEXX SNAP 4Dx® test in detecting canine anaplasmosis/ehrlichiosis in Trinidad","authors":"Ansarah Hosein ,&nbsp;Candice Sant ,&nbsp;Indira Pargass ,&nbsp;Gowrie Lalla ,&nbsp;Fatima Mohammed ,&nbsp;Karla Georges","doi":"10.1016/j.ttbdis.2025.102517","DOIUrl":"10.1016/j.ttbdis.2025.102517","url":null,"abstract":"<div><div><em>Ehrlichia canis</em> and <em>Anaplasma platys</em> are known to be among the most common tick-borne hemopathogens of dogs in Trinidad that are transmitted by ixodid ticks of the <em>Rhipicephalus sanguineus</em> sensu lato species complex<em>.</em> These pathogens are commonly diagnosed in Trinidad based on clinical signs, laboratory tests and response to treatment. However, as these hemopathogens are often not observed on microscopic examination of blood smears, alternative methods to provide a definitive diagnosis are warranted. The IDEXX SNAP 4Dx® test is used frequently by veterinarians in Trinidad to determine exposure status and inform decisions on whether a canine patient should be treated. This study investigated the use of the IDEXX SNAP 4Dx® test and polymerase chain reaction (PCR) followed by a reverse line blot hybridization (RLB) assay on samples obtained from 231 dogs in order to determine if there was an acceptable level of agreement between the two tests. The study showed poor agreement between the IDEXX SNAP 4Dx® test and RLB.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 5","pages":"Article 102517"},"PeriodicalIF":3.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission 边缘无原体调节参与病原体传播的微型鼻多头器官的微生物群
IF 3.1 2区 医学 Q2 INFECTIOUS DISEASES Pub Date : 2025-07-23 DOI: 10.1016/j.ttbdis.2025.102522
Agustina E. Pérez , Eliana C. Guillemi , Lianet Abuin-Denis , Elianne Piloto-Sardiñas , Dasiel Obregon , Natalia Pin Viso , Néstor Sarmiento , Alejandro Cabezas-Cruz , Marisa D. Farber
The cattle tick Rhipicephalus microplus is a vector for Anaplasma marginale in tropical and subtropical regions worldwide. The study of pathogen-microbiome-vector interactions at the tick organ scale is a promising area of development, unraveling control strategies for tick-borne diseases. Our study focused on how A. marginale infection affects the bacterial community within the salivary glands and ovaries of R. microplus. Engorged female ticks collected from cattle in a disease-endemic area of Argentina were further classified based on whether they were infected with A. marginale by targeting the msp1β gene through PCR diagnosis in tissue samples. We included negative control samples throughout the study. We analyzed the bacterial communities in tick tissues by sequencing the 16S rRNA V3-V4 region. Our results revealed significant differences in community composition between infected and uninfected samples. Sphingomonadaceae was identified as a predominant taxon in uninfected salivary glands and ovaries. Additionally, we constructed co-occurrence networks to study interactions within the microbial communities. It is noteworthy that A. marginale infection led to an increase in network complexity in the salivary glands, exerting the opposite effect on the ovaries. These findings reinforced the hypothesis that A. marginale impacts the microbiota of R. microplus at an organ-specific level.
牛蜱微型鼻头虫是世界热带和亚热带地区边缘性无形体病媒。在蜱器官尺度上研究病原体-微生物组-媒介相互作用是一个有前途的发展领域,揭示了蜱传疾病的控制策略。我们的研究主要集中在边缘芽孢杆菌感染如何影响小毛鼠唾液腺和卵巢内的细菌群落。在阿根廷某疾病流行区采集的牛身上采集的雌性蜱虫,通过组织样本的PCR诊断,以msp1β基因为靶点,进一步对其是否感染边缘蜱进行分类。我们在整个研究过程中纳入了阴性对照样本。我们通过测序16S rRNA V3-V4区分析蜱组织细菌群落。我们的结果显示感染和未感染样本之间的群落组成有显著差异。Sphingomonadaceae是未感染的唾液腺和卵巢的优势分类群。此外,我们构建了共生网络来研究微生物群落内部的相互作用。值得注意的是,边缘芽孢杆菌感染导致唾液腺网络复杂性增加,对卵巢产生相反的影响。这些发现强化了一种假设,即边缘拟南芥在器官特异性水平上影响微加罗汉菌的微生物群。
{"title":"Anaplasma marginale modulates the microbiota of Rhipicephalus microplus organs involved in pathogen transmission","authors":"Agustina E. Pérez ,&nbsp;Eliana C. Guillemi ,&nbsp;Lianet Abuin-Denis ,&nbsp;Elianne Piloto-Sardiñas ,&nbsp;Dasiel Obregon ,&nbsp;Natalia Pin Viso ,&nbsp;Néstor Sarmiento ,&nbsp;Alejandro Cabezas-Cruz ,&nbsp;Marisa D. Farber","doi":"10.1016/j.ttbdis.2025.102522","DOIUrl":"10.1016/j.ttbdis.2025.102522","url":null,"abstract":"<div><div>The cattle tick <em>Rhipicephalus microplus</em> is a vector for <em>Anaplasma marginale</em> in tropical and subtropical regions worldwide. The study of pathogen-microbiome-vector interactions at the tick organ scale is a promising area of development, unraveling control strategies for tick-borne diseases. Our study focused on how <em>A. marginale</em> infection affects the bacterial community within the salivary glands and ovaries of <em>R. microplus</em>. Engorged female ticks collected from cattle in a disease-endemic area of Argentina were further classified based on whether they were infected with <em>A. marginale</em> by targeting the <em>msp1β</em> gene through PCR diagnosis in tissue samples. We included negative control samples throughout the study. We analyzed the bacterial communities in tick tissues by sequencing the <em>16S rRNA</em> V3-V4 region. Our results revealed significant differences in community composition between infected and uninfected samples. <em>Sphingomonadaceae</em> was identified as a predominant taxon in uninfected salivary glands and ovaries. Additionally, we constructed co-occurrence networks to study interactions within the microbial communities. It is noteworthy that <em>A. marginale</em> infection led to an increase in network complexity in the salivary glands, exerting the opposite effect on the ovaries. These findings reinforced the hypothesis that <em>A. marginale</em> impacts the microbiota of <em>R. microplus</em> at an organ-specific level.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 5","pages":"Article 102522"},"PeriodicalIF":3.1,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144686099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hyalomma rufipes of Asian origin transported to Europe by a migrant bird 原产于亚洲的斑纹透明瘤由候鸟传播到欧洲
IF 3.1 2区 医学 Q2 INFECTIOUS DISEASES Pub Date : 2025-07-22 DOI: 10.1016/j.ttbdis.2025.102518
Attila D. Sándor , Áron Péter , Joanna B. Wong , Reto Burri , Sándor Hornok
Hyalomma rufipes is one of the most epidemiologically important ticks in Africa and the Middle East. It is regularly transported by migratory birds and there are chances that these ticks may become sources for the emergence of resident populations in the temperate region due to recent changes in climatic conditions. In May 2023, a Hylomma sp. nymph was collected in SE Romania from a long-distance migrant host, the Pied Wheatear (Oenanthe pleschanka) with known migratory route. The tick was identified morphologically and genetically as H. rufipes and based on the timing of the migratory track it attached to its avian host in Saudi Arabia, the Middle East. This is the first ever H. rufipes recorded in Europe with known Middle Eastern/Asian origin.
在非洲和中东地区,rufipes是最重要的流行病学蜱虫之一。它经常由候鸟传播,由于最近气候条件的变化,这些蜱虫有可能成为温带地区常住人口出现的来源。2023年5月,在罗马尼亚东南部采集了一只Hylomma sp.若虫,其寄主为具有已知迁徙路线的斑小麦耳(Oenanthe pleschanka)。根据它附着在中东沙特阿拉伯的鸟类宿主上的迁徙路线的时间,从形态学和遗传学上确定了这种蜱为红唇蜱。这是有史以来第一次记录在欧洲与已知的中东/亚洲起源。
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引用次数: 0
A new hotspot of tick-borne encephalitis virus (TBEV) in the Autonomous Province of Trento, Italy 意大利特伦托自治省蜱传脑炎病毒(TBEV)新热点
IF 3.1 2区 医学 Q2 INFECTIOUS DISEASES Pub Date : 2025-07-18 DOI: 10.1016/j.ttbdis.2025.102513
Giulia Ferrari , Fausta Rosso , Matteo Girardi , Francesca Dagostin , Daniele Arnoldi , Maria Grazia Zuccali , Chiara Mocellin , Silvia Molinaro , Valentina Tagliapietra , Annapaola Rizzoli
The tick-borne encephalitis virus (TBEV) has expanded its distributional range in Europe over recent decades. Italy is considered a low tick-borne encephalitis (TBE) incidence country, nonetheless, human cases have recently increased, reaching their maximum so far in 2022. This study reports on a new TBE hotspot in the Autonomous Province of Trento (Italy), along with a description of TBEV genetic variants at the provincial level. Tick sampling was performed where several TBE human cases were reported in 2022. As a result, 458 Ixodes ricinus ticks were collected (38 females, 49 males, 371 nymphs) and screened for TBEV through real-time PCR, pooling the nymphs while analyzing the adults individually. Eight samples were found to be positive for TBEV, corresponding to a 4.6 % prevalence in adults and a 1.1 % minimum infection rate in nymphs in the area. The identified TBEV genetic variants were compared with other TBEV European subtype (TBEV-Eu) strains. The relatively high prevalence in ticks and the reported human cases beyond the year of vector collection suggest a sustained virus circulation. Additionally, phylogenetic analysis of the genetic variants in the Trentino Province shows a separation among eastern and western sites. This study highlights the crucial importance of active surveillance for mitigating TBE risk, especially in highly anthropized Alpine areas.
近几十年来,蜱传脑炎病毒(TBEV)在欧洲扩大了其分布范围。意大利被认为是低蜱传脑炎发病率的国家,然而,人间病例最近有所增加,在2022年达到迄今为止的最高水平。本研究报告了Trento自治省(意大利)的一个新的TBEV热点,以及省级TBEV遗传变异的描述。在2022年报告了几例TBE人间病例的地方进行了蜱虫取样。结果采集蓖麻蜱458只(雌蜱38只,雄蜱49只,雌雄蜱371只),采用实时荧光定量PCR技术进行TBEV检测,雌雄蜱集中,成虫单独分析。8个样本被发现为TBEV阳性,相当于该地区成人感染率为4.6%,若虫感染率最低为1.1%。将鉴定的TBEV遗传变异与其他TBEV欧洲亚型(TBEV- eu)进行比较。蜱虫中相对较高的流行率以及在媒介收集年份之后报告的人间病例表明存在持续的病毒传播。此外,对特伦蒂诺省遗传变异的系统发育分析表明,东部和西部位点存在分离。这项研究强调了主动监测对于降低TBE风险的重要性,特别是在高度人性化的高山地区。
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引用次数: 0
First record of Haemaphysalis concinna (Acari: Ixodidae) tick in the Khalkh Numrug basin of Eastern Mongolia 在蒙古东部的Khalkh Numrug盆地首次记录到中国血蜱(蜱螨目:伊蚊科)
IF 3.1 2区 医学 Q2 INFECTIOUS DISEASES Pub Date : 2025-07-18 DOI: 10.1016/j.ttbdis.2025.102521
Banzragch Battur , Batsaikhan Enkhtaivan , Davaajav Otgonsuren , Batbold Davkharbayar , Dalantai Munkhgerel , Tovuu Amgalanbaatar , Sandagdorj Narantsatsral , Batdorj Davaasuren , Myagmar Zoljargal , Punsantsogvoo Myagmarsuren , Orkhon Banzragchgarav , Noboru Inoue , Naoaki Yokoyama , Jinlin Zhou , Miroslav Bobek , Badgar Battsetseg
Haemaphysalis (Acari: Ixodidae) ticks remain poorly studied in Mongolia. Here, we report the first confirmed record of Haemaphysalis concinna Koch, 1844 in the Numrug Strictly Protected Area, Dornod Province, eastern Mongolia, based on both morphological characteristics and molecular analyses. This region lies along the Mongolia-China border within the forest-steppe and steppe zones of the western Greater Khingan Mountains. Tick sampling was conducted at six sites (lakeshores, riverbanks, and marshy forests) between early April and mid-August over a three-year period.
A total of 1014 H concinna specimens were collected, of which 148 (including two nymphs) were selected for DNA extraction. PCR amplifications targeting the mitochondrial 16S rRNA (∼455 bp) and Cox1 (∼820 bp) genes were performed. Eight samples were sequenced, with three 16S and two Cox1 sequences meeting quality thresholds. The sequences of both genes exhibited 99.8 % and 100 % identity, respectively, to H. concinna sequences from Russia (Cox1: PP851089.1, PP851095.1; 16S rRNA: KP866207.1) and China (Cox1: KR108863.1; 16S rRNA: ON097130.1) available in GenBank. This high level of sequence identity strongly supports the identification of the Mongolian samples as H. concinna. Species identification was further supported by morphological characteristics consistent with published descriptions of adult ticks. This record from the Khalkh Numrug basin represents the first verified occurrence of H. concinna in eastern Mongolia. These findings underscore the need for further research on the species’ ecology, host range, and potential public health significance in the region.
蒙古对血蜱(蜱螨属:伊蚊科)的研究仍然很少。本文报道了蒙古国东部多诺德省努鲁格严格保护区首次确认的coninna Koch血蜱,1844,基于形态特征和分子分析。该地区位于蒙中边境,位于大兴安岭西部的森林草原和草原地带。在4月初至8月中旬的三年时间里,在六个地点(湖岸、河岸和沼泽森林)进行了蜱虫取样。共采集标本1014只,选取148只(含2只若虫)进行DNA提取。对线粒体16S rRNA (~ 455 bp)和Cox1 (~ 820 bp)基因进行PCR扩增。对8个样本进行测序,其中3个16S序列和2个Cox1序列符合质量阈值。这两个基因序列分别与来自俄罗斯的H. concina序列(Cox1: PP851089.1, PP851095.1;16S rRNA: KP866207.1)和中国(Cox1: KR108863.1;16S rRNA: ON097130.1),可在GenBank中找到。这种高水平的序列同一性有力地支持了蒙古样品为红毛猴的鉴定。与已发表的成蜱描述一致的形态特征进一步支持了物种鉴定。来自Khalkh Numrug盆地的这一记录代表了蒙古东部首次证实的H. concina的出现。这些发现强调需要进一步研究该物种的生态、宿主范围和潜在的公共卫生意义。
{"title":"First record of Haemaphysalis concinna (Acari: Ixodidae) tick in the Khalkh Numrug basin of Eastern Mongolia","authors":"Banzragch Battur ,&nbsp;Batsaikhan Enkhtaivan ,&nbsp;Davaajav Otgonsuren ,&nbsp;Batbold Davkharbayar ,&nbsp;Dalantai Munkhgerel ,&nbsp;Tovuu Amgalanbaatar ,&nbsp;Sandagdorj Narantsatsral ,&nbsp;Batdorj Davaasuren ,&nbsp;Myagmar Zoljargal ,&nbsp;Punsantsogvoo Myagmarsuren ,&nbsp;Orkhon Banzragchgarav ,&nbsp;Noboru Inoue ,&nbsp;Naoaki Yokoyama ,&nbsp;Jinlin Zhou ,&nbsp;Miroslav Bobek ,&nbsp;Badgar Battsetseg","doi":"10.1016/j.ttbdis.2025.102521","DOIUrl":"10.1016/j.ttbdis.2025.102521","url":null,"abstract":"<div><div><em>Haemaphysalis</em> (Acari: Ixodidae) ticks remain poorly studied in Mongolia. Here, we report the first confirmed record of <em>Haemaphysalis concinna</em> Koch, 1844 in the Numrug Strictly Protected Area, Dornod Province, eastern Mongolia, based on both morphological characteristics and molecular analyses. This region lies along the Mongolia-China border within the forest-steppe and steppe zones of the western Greater Khingan Mountains. Tick sampling was conducted at six sites (lakeshores, riverbanks, and marshy forests) between early April and mid-August over a three-year period.</div><div>A total of 1014 <em>H concinna</em> specimens were collected, of which 148 (including two nymphs) were selected for DNA extraction. PCR amplifications targeting the mitochondrial 16S rRNA (∼455 bp) and <em>Cox1</em> (∼820 bp) genes were performed. Eight samples were sequenced, with three 16S and two <em>Cox1</em> sequences meeting quality thresholds. The sequences of both genes exhibited 99.8 % and 100 % identity, respectively, to <em>H. concinna</em> sequences from Russia (<em>Cox1</em>: PP851089.1, PP851095.1; 16S rRNA: KP866207.1) and China (<em>Cox1</em>: KR108863.1; 16S rRNA: ON097130.1) available in GenBank. This high level of sequence identity strongly supports the identification of the Mongolian samples as <em>H. concinna</em>. Species identification was further supported by morphological characteristics consistent with published descriptions of adult ticks. This record from the Khalkh Numrug basin represents the first verified occurrence of <em>H. concinna</em> in eastern Mongolia. These findings underscore the need for further research on the species’ ecology, host range, and potential public health significance in the region.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 5","pages":"Article 102521"},"PeriodicalIF":3.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IxsS7: A novel biomarker for Ixodes scapularis tick bite exposure in humans IxsS7:一种新的肩胛骨蜱叮咬暴露生物标志物
IF 3.1 2区 医学 Q2 INFECTIOUS DISEASES Pub Date : 2025-07-01 DOI: 10.1016/j.ttbdis.2025.102514
Patrick H. Kelly , Derek A.T. Cummings , Eva Iniguez , Tiago Donatelli , Luana Rogerio , Jan Kotál , Larissa Almeida Martins , Markus Berger , Lucas C Sousa-Paula , Stephen Lu , James H. Stark , Siu-Ping Ng , Adriana Marques , Jesus G. Valenzuela , Lucas Tirloni
Ixodes scapularis is a primary vector of several important tick-borne pathogens including Borrelia burgdorferi sensu lato, the causative bacterial genospecies complex of Lyme disease, Babesia microti, Anaplasma phagocytophilum, Borrelia miyamotoi, Ehrlichia muris eauclarensis, and Powassan virus. Salivary compounds secreted by I. scapularis during blood feeding are immunogenic and can elicit robust antibody responses in humans which can potentially be leveraged as surrogate markers of prior tick bite exposure. In this study, we investigate the potential of a tick secreted salivary serine protease inhibitor, IxsS7, as a novel antigenic biomarker of I. scapularis exposure in humans. We demonstrate that the IxsS7 protein-coding sequence is highly conserved (>90 % identity) among other important Ixodes species (e.g., Ixodes ricinus, Ixodes persulcatus, and Ixodes pacificus) and poorly conserved (<50 % identity) with homologs from other tick genera, such as Amblyomma spp., Dermacentor spp., Rhipicephalus spp., and Haemaphysalis spp. Antibodies in sera from rabbits immunized with recombinant IxsS7 (rIxsS7) strongly recognize native IxsS7 when challenged with salivary gland homogenate (SGH) from blood-fed I. scapularis females, while showing minimal cross-reactivity with SGH from other hard tick (Ixodidae) genera. Western blot and ELISA analyses revealed that human subjects who reported recent prior exposure to ticks possessed IgG antibodies that recognized rIxsS7, highlighting its potential as a biomarker of exposure specifically against I. scapularis. Further development of serological tools that can measure human antibody responses to Ixodes-specific salivary antigens is essential to better quantify individual- and population-level risk of important tick-borne diseases such as Lyme disease.
肩胛骨硬体是几种重要蜱传病原体的主要媒介,包括感伯氏疏螺旋体、莱姆病的致病细菌基因种复合体、微小巴贝斯虫、嗜吞噬细胞无形体、宫本氏疏螺旋体、穆拉氏埃利希体和波瓦桑病毒。肩胛骨蜱在吸血过程中分泌的唾液化合物具有免疫原性,可在人体中引起强大的抗体反应,这可能被用作以前蜱叮咬暴露的替代标记。在这项研究中,我们研究了蜱分泌唾液丝氨酸蛋白酶抑制剂IxsS7作为人类暴露于肩胛骨恙虫的新型抗原生物标志物的潜力。研究表明,IxsS7蛋白编码序列在其他重要蜱属(如蓖麻蜱、过弯蜱和太平洋蜱)中高度保守(同源性为90%),而与其他蜱属(如Amblyomma spp.、Dermacentor spp.、Rhipicephalus spp.)的同源物保守性较差(同源性为50%)。重组IxsS7 (rIxsS7)免疫家兔的血清抗体在被血喂养的雌性肩胛骨蜱的唾液腺浆液(SGH)攻击时强烈识别原生IxsS7,而与其他硬蜱(伊蚊科)属的SGH表现出最小的交叉反应性。Western blot和ELISA分析显示,报告最近接触过蜱虫的人类受试者具有识别rIxsS7的IgG抗体,突出了其作为特异性暴露于肩胛骨恙虫的生物标志物的潜力。进一步开发血清学工具,以测量人类对伊蚊特异性唾液抗原的抗体反应,这对于更好地量化个体和人群层面的重要蜱传疾病(如莱姆病)风险至关重要。
{"title":"IxsS7: A novel biomarker for Ixodes scapularis tick bite exposure in humans","authors":"Patrick H. Kelly ,&nbsp;Derek A.T. Cummings ,&nbsp;Eva Iniguez ,&nbsp;Tiago Donatelli ,&nbsp;Luana Rogerio ,&nbsp;Jan Kotál ,&nbsp;Larissa Almeida Martins ,&nbsp;Markus Berger ,&nbsp;Lucas C Sousa-Paula ,&nbsp;Stephen Lu ,&nbsp;James H. Stark ,&nbsp;Siu-Ping Ng ,&nbsp;Adriana Marques ,&nbsp;Jesus G. Valenzuela ,&nbsp;Lucas Tirloni","doi":"10.1016/j.ttbdis.2025.102514","DOIUrl":"10.1016/j.ttbdis.2025.102514","url":null,"abstract":"<div><div><em>Ixodes scapularis</em> is a primary vector of several important tick-borne pathogens including <em>Borrelia burgdorferi</em> sensu lato, the causative bacterial genospecies complex of Lyme disease, <em>Babesia microti, Anaplasma phagocytophilum, Borrelia miyamotoi, Ehrlichia muris eauclarensis,</em> and Powassan virus. Salivary compounds secreted by <em>I. scapularis</em> during blood feeding are immunogenic and can elicit robust antibody responses in humans which can potentially be leveraged as surrogate markers of prior tick bite exposure. In this study, we investigate the potential of a tick secreted salivary serine protease inhibitor, <em>Ixs</em>S7, as a novel antigenic biomarker of <em>I. scapularis</em> exposure in humans. We demonstrate that the <em>Ixs</em>S7 protein-coding sequence is highly conserved (&gt;90 % identity) among other important <em>Ixodes</em> species (e.g., <em>Ixodes ricinus, Ixodes persulcatus</em>, and <em>Ixodes pacificus</em>) and poorly conserved (&lt;50 % identity) with homologs from other tick genera, such as <em>Amblyomma</em> spp., <em>Dermacentor</em> spp., <em>Rhipicephalus</em> spp., and <em>Haemaphysalis</em> spp. Antibodies in sera from rabbits immunized with recombinant <em>Ixs</em>S7 (r<em>Ixs</em>S7) strongly recognize native <em>Ixs</em>S7 when challenged with salivary gland homogenate (SGH) from blood-fed <em>I. scapularis</em> females, while showing minimal cross-reactivity with SGH from other hard tick (Ixodidae) genera. Western blot and ELISA analyses revealed that human subjects who reported recent prior exposure to ticks possessed IgG antibodies that recognized r<em>Ixs</em>S7, highlighting its potential as a biomarker of exposure specifically against <em>I. scapularis.</em> Further development of serological tools that can measure human antibody responses to <em>Ixodes</em>-specific salivary antigens is essential to better quantify individual- and population-level risk of important tick-borne diseases such as Lyme disease.</div></div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"16 4","pages":"Article 102514"},"PeriodicalIF":3.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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Ticks and Tick-borne Diseases
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