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A novel hand-eye calibration method for industrial robot and line laser vision sensor 一种新的工业机器人手眼标定方法及线激光视觉传感器
IF 1.6 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2023-05-31 DOI: 10.1108/sr-09-2022-0357
Xu Jingbo, Li Qiaowei, White Bai
PurposeThe purpose of this study is solving the hand–eye calibration issue for line structured light vision sensor. Only after hand–eye calibration the sensor measurement data can be applied to robot system.Design/methodology/approachIn this paper, the hand–eye calibration methods are studied, respectively, for eye-in-hand and eye-to-hand. Firstly, the coordinates of the target point in robot system are obtained by tool centre point (TCP), then the robot is controlled to make the sensor measure the target point in multiple poses and the measurement data and pose data are obtained; finally, the sum of squared calibration errors is minimized by the least square method. Furthermore, the missing vector in the process of solving the transformation matrix is obtained by vector operation, and the complete matrix is obtained.FindingsOn this basis, the sensor measurement data can be easily and accurately converted to the robot coordinate system by matrix operation.Originality/valueThis method has no special requirement for robot pose control, and its calibration process is fast and efficient, with high precision and has practical popularized value.
目的解决线结构光视觉传感器的手眼标定问题。传感器的测量数据只有经过手眼标定后才能应用到机器人系统中。设计/方法/方法本文分别研究了眼对手和眼对手的手眼标定方法。首先通过刀具中心点(TCP)获取机器人系统中目标点的坐标,然后控制机器人使传感器对目标点进行多姿态测量,获得测量数据和姿态数据;最后,采用最小二乘法最小化标定误差平方和。通过向量运算得到变换矩阵求解过程中缺失的向量,得到完整的矩阵。在此基础上,通过矩阵运算可以方便、准确地将传感器测量数据转换为机器人坐标系。独创性/价值该方法对机器人姿态控制无特殊要求,标定过程快速高效,精度高,具有实用推广价值。
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引用次数: 0
Design of magnetostrictive tactile sensor for depth detection 用于深度检测的磁致伸缩触觉传感器的设计
IF 1.6 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2023-05-19 DOI: 10.1108/sr-01-2022-0051
Ling Weng, Zhuolin Li, Xu Luo, Yuanye Zhang, Yang Liu
PurposeThis paper aims to design a magnetostrictive tactile sensor for surface depth detection. Unlike the human finger, although most tactile sensors have high sensitivity to pressure, they cannot detect millimeter-level depth information on the surface of objects precisely. To enhance the ability to detect surface depth information, a piezomagnetic sensor combining inverse magnetostrictive effect and bionic structure is developed in this paper.Design/methodology/approachA magnetostrictive tactile sensor based on Galfenol [(Fe83Ga17)99.4B0.6] is designed and studied for surface depth measurement. The optimal structure of the sensor is determined by experiment and theory. The test platforms for static and dynamic characteristics are set up. The static and the dynamic sensing performance of the sensor are studied experimentally.FindingsThe sensor can detect 0–2 mm depth change with a sensitivity of 91.5 mV/mm. A resolution of 50 µm can be achieved in the depth direction. In 50 cycles of loading and unloading tests, the maximum error of the sensor output voltage amplitude is only 2.23%.Originality/valueThe sensor can measure the depth information of object surface precisely with good repeatability through sliding motion and provide reference for object surface topography detection.
目的设计一种用于表面深度检测的磁致伸缩触觉传感器。与人类手指不同,尽管大多数触觉传感器对压力具有高灵敏度,但它们无法精确检测物体表面的毫米级深度信息。为了提高表面深度信息的检测能力,本文开发了一种结合反磁致伸缩效应和仿生结构的压磁传感器。设计/方法/方法设计并研究了一种基于Galfenol[(Fe83Ga17)99.4B0.6]的磁致伸缩触觉传感器,用于表面深度测量。通过实验和理论确定了传感器的最佳结构。建立了静态和动态特性测试平台。对传感器的静态和动态传感性能进行了实验研究。查找传感器可以检测0–2 mm深度变化,灵敏度为91.5 mV/mm。50的分辨率 在深度方向上可以达到µm。在50个循环的加载和卸载测试中,传感器输出电压幅度的最大误差仅为2.23%。独创性/值传感器可以通过滑动运动以良好的重复性精确测量物体表面的深度信息,为物体表面形貌检测提供参考。
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引用次数: 1
Development of an ultrasonic linear encoder 超声线性编码器的研制
IF 1.6 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2023-05-05 DOI: 10.1108/sr-07-2022-0262
Chung-Ping Chang, Song-Fu Hong, Tzu-Guang Chen
PurposeIn this investigation, a linear encoder system based on the ultrasonic transducer has been proposed. Ultrasonic transducers are usually designed for distance measurements, such as the time of flight method and sonar system. These applications are defined as discrete-length measurement technologies. The purpose of this study is to develop a continuous displacement measurement system using ultrasonic transducers.Design/methodology/approachA modified signal processing based on heterodyne signaling is implemented in this system. In the proposed signal processing, there is an automatic gain control module, a phase-shifting module, a phase detection module, an interpolation module and especially a frequency multiplication module, which can enhance the resolution and reduce the interpolation error simultaneously.FindingsThe proposed system can generate the encoding signals and is compatible with most motion control systems. For the experimental result, the maximum measurement error and standard deviation are about −0.027 and 0.048 mm, respectively. It shows that the proposed encoder system has the potential for displacement measurement tasks.Originality/valueThis study reveals an ultrasonic linear encoder that is capable of generating an incremental encoding signal, accompanied by a corresponding signal processing methodology. In contrast to the conventional heterodyne signal processing approach, the proposed multiplication method effectively reduces the interpolation error that arises because of multiple reflections.
目的提出了一种基于超声换能器的线性编码器系统。超声波换能器通常设计用于距离测量,如飞行时间法和声纳系统。这些应用被定义为离散长度测量技术。本研究的目的是开发一种利用超声波换能器的连续位移测量系统。该系统实现了一种基于外差信号的改进信号处理方法。在所提出的信号处理中,有自动增益控制模块、移相模块、相位检测模块、插补模块,尤其是乘频模块,可以在提高分辨率的同时减小插补误差。研究结果:该系统能够生成编码信号,并与大多数运动控制系统兼容。实验结果的最大测量误差和标准偏差分别约为- 0.027和0.048 mm。结果表明,该编码器系统具有实现位移测量任务的潜力。原创性/价值本研究揭示了一种超声线性编码器,该编码器能够产生增量编码信号,并伴有相应的信号处理方法。与传统的外差信号处理方法相比,该方法有效地降低了由于多次反射而产生的插值误差。
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引用次数: 1
A multi-sensor based emergency healthcare monitoring system integrating heart status, stress, and alcohol detections 一个基于多传感器的紧急医疗监测系统,集成了心脏状态、压力和酒精检测
IF 1.6 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2023-05-03 DOI: 10.1108/sr-03-2022-0136
Karandeep Kaur, H. Verma
PurposeUbiquitous health-care monitoring systems can provide continuous surveillance to a person using various sensors, including wearables and implantable and fabric-woven sensors. By assessing the state of many physiological characteristics of the patient’s body, continuous monitoring can assist in preparing for the impending emergency. To address this issue, this study aims to propose a health-care system that integrates the treatment of the impending heart, stress and alcohol emergencies. For this purpose, this study uses readings from sensors used for electrocardiography, heart rate, respiration rate, blood alcohol content percentage and blood pressure of a patient’s body.Design/methodology/approachFor heart status, stress level and alcohol detection, the parametric values obtained from these sensors are preprocessed and further divided into four, five and six phases, respectively. A final integrated emergency stage is derived from the stages that were interpreted to examine at a person’s state of emergency. A thorough analysis of the proposed model is carried out using four classification techniques, including decision trees, support vector machines, k nearest neighbors and ensemble classifiers. For all of the aforementioned detections, four metrics are used to evaluate performance: classification accuracy, precision, recall and fmeasure.FindingsEventually, results are validated against the existing health-care systems. The empirical results received reveal that the proposed model outperforms the existing health-care models in the context of metrics above for different detections taken into consideration.Originality/valueThis study proposes a health-care system capable of performing data processing using wearable sensors. It is of great importance for real-time systems. This study assures the originality of the proposed system.
目的无处不在的医疗保健监测系统可以使用各种传感器为人们提供持续的监测,包括可穿戴设备、植入式和织物编织传感器。通过评估患者身体的许多生理特征的状态,持续监测可以帮助为即将到来的紧急情况做好准备。为了解决这个问题,本研究旨在提出一个医疗保健系统,将即将到来的心脏、压力和酒精紧急情况的治疗结合起来。为此,这项研究使用了用于心电图、心率、呼吸率、血液酒精含量百分比和患者身体血压的传感器读数。设计/方法/方法对于心脏状态、压力水平和酒精检测,从这些传感器获得的参数值被预处理,并分别进一步分为四个、五个和六个阶段。最后的综合应急阶段是从被解释为在个人紧急状态下进行检查的阶段派生出来的。使用四种分类技术,包括决策树、支持向量机、k近邻和集成分类器,对所提出的模型进行了深入分析。对于上述所有检测,使用四个指标来评估性能:分类准确性、精确度、召回率和fmeasure。结果最终,将结果与现有的医疗保健系统进行对比验证。收到的经验结果表明,在考虑不同检测的上述指标的背景下,所提出的模型优于现有的医疗保健模型。独创性/价值这项研究提出了一种能够使用可穿戴传感器进行数据处理的医疗保健系统。它对实时系统具有重要意义。这项研究确保了所提出的系统的独创性。
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引用次数: 0
A flexible pressure sensor with interference immunity capability 一种具有抗干扰能力的柔性压力传感器
IF 1.6 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2023-05-02 DOI: 10.1108/sr-12-2021-0487
Huan Liu, Rui Wang, Junyao Wang, Xingyu Chen, Yunpeng Li, Bowen Cui, Tianhong Lang, Weihua Zhu
PurposeFlexible pressure sensor arrays have promising applications in analog haptics, reconfiguration of sensory functions, artificial intelligence, wearable devices and human-computer interaction. The force disturbance generated by the connecting material between the sensor array units will reduce the detection accuracy of the unit. The purpose of this paper is to propose a flexible pressure sensor with interference immunity capability. A C-type bridge flexible piezoelectric structure is used to improve the pressure perturbation. The interference immunity capability of the sensor has been improved.Design/methodology/approachIn this paper, a C-type pressure sensor array structure by rapid injection moulding is manufactured through the positive piezoelectric effect of a piezoelectric material. The feasibility of C-type interference immunity structure in a flexible sensor array is verified by further analysis and experiment. A flexible pressure sensor array with C-type interference immunity structure has been proposed.FindingsIn this paper, we present the results of the perturbation experiment results of the C-type pressure sensor array, showing that the perturbation error is less than 8%. The test of the flexible sensor array show that the sensor can identify the curved angle of up to 120 °, and the output sensitivity of the sensor in the horizontal state reaches 0.12 V/N, and the sensor can withstand the pressure of 80 N. The flexible sensor can work stably in the stretch rate range of 0–8.6% and the stretch length range of 0–6 mm.Originality/valueIn this paper, C-type pressure sensor array structure is fabricated by rapid injection moulding for the first time. The research in this paper can effectively reduce the disturbance of input pressure on the sensor’s internal array and improve the output accuracy. The sensor can intuitively reflect the number of fingers sliding on the sensor by the order in which the maximum voltage appears. Due to the strong interference immunity capability and flexibility of the flexible sensor array mechanism, it has a broad application prospect in the practical fields of haptic simulation, perceptual function reconstruction, artificial intelligence, wearable devices and human–computer interaction.
目的柔性压力传感器阵列在模拟触觉、传感功能重构、人工智能、可穿戴设备和人机交互等领域具有广阔的应用前景。传感器阵列单元之间的连接材料产生的力扰动会降低单元的检测精度。本文的目的是提出一种具有抗干扰能力的柔性压力传感器。采用C型桥柔性压电结构来改善压力扰动。传感器的抗干扰能力得到了提高。设计/方法/方法本文通过压电材料的正压电效应,通过快速注射成型制造了C型压力传感器阵列结构。通过进一步的分析和实验验证了C型抗干扰结构在柔性传感器阵列中的可行性。提出了一种具有C型抗干扰结构的柔性压力传感器阵列。本文给出了C型压力传感器阵列的微扰实验结果,表明微扰误差小于8%。柔性传感器阵列的测试表明,该传感器可以识别高达120°的弯曲角度,传感器在水平状态下的输出灵敏度达到0.12 V/N,传感器可以承受80的压力 N.柔性传感器可以在0–8.6%的拉伸率范围和0–6的拉伸长度范围内稳定工作 mm.原始性/值本文首次采用快速注射成型的方法制备了C型压力传感器阵列结构。本文的研究可以有效地减少输入压力对传感器内部阵列的干扰,提高输出精度。传感器可以按照最大电压出现的顺序直观地反映手指在传感器上滑动的数量。由于柔性传感器阵列机构具有较强的抗干扰能力和灵活性,在触觉仿真、感知功能重建、人工智能、可穿戴设备和人机交互等实际领域具有广阔的应用前景。
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引用次数: 1
Comparison of different reinforcement methods on crack monitoring characteristics of eddy current array sensor 不同加固方法对涡流阵列传感器裂纹监测特性的比较
IF 1.6 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2023-05-02 DOI: 10.1108/sr-09-2022-0355
Xianghong Fan, Tao Chen, Yuting He
PurposeThis paper aims to study the influence of different reinforcement methods on crack monitoring characteristics of eddy current array sensors, and the sensors with two different reinforcement methods, SUS304 reinforcement and permalloy reinforcement, are proposed.Design/methodology/approachFirst, the finite element model of the sensor is established to analyze the influence of the reinforcement plate’s electromagnetic parameters on the crack identification sensitivity. Then, the crack monitoring accuracy test of sensors with two reinforcement methods is carried out. Finally, the fatigue crack monitoring experiments with bolt tightening torques of 45 and 63 N · m are carried out, respectively.FindingsIn this study, it is found that the crack identification sensitivity of the sensor can be improved by increasing the relative permeability of the reinforcement plate. The crack monitoring accuracy of the sensors with two different reinforcement methods is about 1 mm. And the crack identification sensitivity of the sensor reinforced by permalloy reinforcement plate is significantly higher than that of the sensor reinforced by SUS304 reinforcement plate.Originality/valueThe sensor reinforced by reinforcement plate can work normally under the squeezing action of the bolt, and the crack monitoring sensitivity of the sensor can be significantly improved by using the reinforcement plate with high relative permeability.
目的研究不同加固方法对涡流阵列传感器裂纹监测特性的影响,提出了SUS304和坡莫合金两种不同加固方法的传感器。设计/方法/方法首先,建立传感器的有限元模型,分析补强板的电磁参数对裂纹识别灵敏度的影响。然后,采用两种加固方法对传感器的裂缝监测精度进行了测试。最后,分别进行了螺栓拧紧力矩为45和63N·m的疲劳裂纹监测实验。研究发现,通过提高补强板的相对渗透性,可以提高传感器的裂纹识别灵敏度。采用两种不同加固方法的传感器的裂缝监测精度约为1 并且坡莫合金增强板增强的传感器的裂纹识别灵敏度显著高于SUS304增强板增强传感器。独创性/价值加固板加固的传感器在螺栓的挤压作用下可以正常工作,使用相对渗透率高的加固板可以显著提高传感器的裂纹监测灵敏度。
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引用次数: 0
Design a custom resistive force sensors to optimize sensorized insoles 设计定制阻力传感器,优化传感鞋垫
IF 1.6 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2023-04-27 DOI: 10.1108/sr-08-2022-0322
D. H. A. Nascimento, F. Magalhães, G. Sabino, R. Resende, M. Duarte, Claysson Bruno Santos Vimieiro
PurposeCurrently, several studies have been published using sensorized insoles for estimating ground reaction force using plantar pressure. However, information on design parameters, manufacturing techniques and guidelines for developing insoles is scarce, often leaving gaps that do not allow reproducing the insole. This study aims to empirically investigate the main parameters of constructing a sensorized insole for application in human gait.Design/methodology/approachTwo devices were built to evaluate the force sensors. The first focuses on the construction of the sensors with different settings: the density of the sensor’s conductive trails (thickness and distance of the trails) and the inertia of the sensors (use of spacers to prevent unwanted readings). The second device focuses on the data capture and processing system: resolution of the analog–digital converter, acquisition rate and sensor activation level.FindingsThe resolution increase of the analog–digital converter and acquisition rate do not contribute to noise increase. Reducing the sensors’ coverage area can increase sensorized insole capacity. The inertia of the sensors can be adjusted using spacers without changing the electrical circuit and acquisition system.Originality/valueMost sensorized insoles use commercial sensors. For this reason, it is not possible a full customization. This paper maps the main variables to manufacture custom sensors and data acquisition systems. This work also presents a case study where it is possible to see the influence of the parameters in the correlation between the sensorized insole and an instrumented treadmill with a force platform.
目的目前,已经发表了几项研究,使用传感鞋垫通过足底压力来估计地面反作用力。然而,有关开发鞋垫的设计参数、制造技术和指南的信息很少,经常留下不允许复制鞋垫的空白。本研究旨在实证研究构建用于人类步态的传感鞋垫的主要参数。设计/方法/方法建造了两个装置来评估力传感器。第一个重点是具有不同设置的传感器的结构:传感器导电迹线的密度(迹线的厚度和距离)和传感器的惯性(使用垫片以防止不必要的读数)。第二个设备专注于数据采集和处理系统:模数转换器的分辨率、采集速率和传感器激活水平。发现模数转换器的分辨率和采集速率的增加不会导致噪声增加。减少传感器的覆盖面积可以增加鞋垫的传感能力。在不改变电路和采集系统的情况下,可以使用垫片来调整传感器的惯性。独创性/价值大多数传感鞋垫都使用商用传感器。因此,不可能进行完全的定制。本文绘制了制造定制传感器和数据采集系统的主要变量。这项工作还提供了一个案例研究,其中可以看到传感鞋垫和带力平台的器械跑步机之间的相关性中参数的影响。
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引用次数: 0
Noise measurement and system calibration on magnetoresistive sensors 磁阻传感器的噪声测量与系统校准
IF 1.6 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2023-04-26 DOI: 10.1108/sr-07-2022-0284
Aiyu Dou, R. Bai, Huachen Zhu, Z. Qian
PurposeThe noise measurement on magnetoresistive (MR) sensors is generally conducted by techniques including single-channel data sampling and fast Fourier transform (FFT) analysis as well as two-channel cross-correlation. The single-channel method is easy to implement and is widely used in the noise measurement on MR sensors, whereas the two-channel method can only eliminate part of the system noise. This study aims to address two key issues affecting measurement accuracy: calibration of the measurement system and the elimination of system noise.Design/methodology/approachThe system is calibrated by using a low-noise metal film resistor in that the system noise is eliminated through power spectrum subtraction. Noise measurement and analysis are conducted for both thermal noise and detectivity of magnetic tunnel junction (MTJ) sensor.FindingsThe thermal noise measurement error is less than 2%. The detectivity of the MTJ sensor reaches 27 pT/Hz1/2 at 2 kHz.Originality/valueThis study provides a more practical solution for noise measurement and system calibration on MR sensors with a bias voltage and magnetic field.
目的磁阻传感器的噪声测量一般采用单通道数据采样、快速傅里叶变换(FFT)分析和双通道互相关等技术。单通道方法易于实现,广泛应用于磁阻传感器的噪声测量中,而双通道方法只能消除部分系统噪声。本研究旨在解决影响测量精度的两个关键问题:测量系统的校准和系统噪声的消除。设计/方法/方法系统采用低噪声金属膜电阻进行校准,通过功率谱减法消除系统噪声。对磁隧道结(MTJ)传感器的热噪声和探测性进行了噪声测量和分析。结果:热噪声测量误差小于2%。在2khz时,MTJ传感器的探测率达到27pt /Hz1/2。独创性/价值本研究为具有偏置电压和磁场的磁磁共振传感器的噪声测量和系统校准提供了一个更实用的解决方案。
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引用次数: 1
Research on thermal protection of piezoelectric pressure sensor for shock wave pressure measurement in explosion field 爆炸现场冲击波压力测量用压电式压力传感器的热保护研究
IF 1.6 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2023-04-26 DOI: 10.1108/sr-11-2022-0407
Yucheng Shi, D. Kong, Xuejiao Ma
PurposeThe purpose of this study is to clarify the mechanism of ambient and transient temperature effects on piezoelectric pressure sensors, and to propose corresponding compensation measures. The temperature of the explosion field has a significant influence on the piezoelectric sensor used to measure the shock wave pressure. For accurate shock wave pressure measurement, based on the actual piezoelectric pressure sensors used in the explosion field, the effects of ambient and transient temperatures on the sensor should be studied.Design/methodology/approachThe compensation method of the ambient temperature is discussed according to the sensor size and material. The theoretical analysis method of the transient temperature is proposed. For the transient temperature conduction problem of the sensor, the finite element simulation method of structure-temperature coupling is used to solve the temperature distribution of the sensor and the change in the contact force on the quartz crystal surface under the step and triangle temperatures. The simulation results are highly consistent with the theory.FindingsBased on the analysis results, a transient temperature control method is proposed, in which 0.5 mm thick lubricating silicone grease is applied to the sensor diaphragm, and 0.2 mm thick fiberglass cloth is wrapped around the sensor side. Simulation experiments are carried out to verify the feasibility of the control method, and the results show that the control method effectively suppresses the output of the thermal parasitic.Originality/valueThe above thermal protection methods can effectively improve the measurement accuracy of shock wave pressure and provide technical support for the evaluation of the power of explosion damage.
目的研究环境温度和瞬态温度对压电压力传感器的影响机理,并提出相应的补偿措施。爆炸场温度对用于测量冲击波压力的压电传感器有重要影响。为了准确测量冲击波压力,应根据爆炸现场实际使用的压电压力传感器,研究环境温度和瞬态温度对传感器的影响。根据传感器的尺寸和材料,讨论了环境温度的补偿方法。提出了瞬态温度的理论分析方法。针对传感器的瞬态温度传导问题,采用结构-温度耦合的有限元模拟方法,求解了传感器的温度分布以及在阶梯温度和三角温度下石英晶体表面接触力的变化。仿真结果与理论结果高度吻合。在分析结果的基础上,提出了一种瞬态温度控制方法,即在传感器膜片上涂上0.5 mm厚的润滑硅脂,在传感器侧面包裹0.2 mm厚的玻璃纤维布。仿真实验验证了该控制方法的可行性,结果表明该控制方法有效地抑制了热寄生的输出。以上热防护方法可有效提高冲击波压力的测量精度,为爆炸损伤威力的评估提供技术支持。
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引用次数: 1
Transition metal salphen DNA complexes as biosensor application for detecting various mosquito viruses 过渡金属噻吩DNA复合物作为生物传感器在检测各种蚊子病毒中的应用
IF 1.6 4区 工程技术 Q3 INSTRUMENTS & INSTRUMENTATION Pub Date : 2023-04-21 DOI: 10.1108/sr-06-2022-0253
M. Monajjemi, Fatemeh Mollaamin
PurposePiperidine side chain-functionalized N, N′-bissalicylidene phenylene di amine di-anion (salphen) consisting of salphen-Zn and salphen-Cu are able to intercalate with nucleic base stacking of DNA and can be applied as an optical DNA hybridization detector. Attaching DNA and salphen to glass surfaces has been done via coating the surface with the silane coupling agents containing 3-aminopropyltriethoxysilane that was synthesized for acting as a high-affinity RNA carrier matrix. The Schiff base salphen-zinc (II) and salphen-Cu (II) complexes-labelled probe to target nucleic acid renders a colour change of the DNA biosensor to a green and red background colour for zinc and copper, respectively. This study aims to indicate that the DNA biosensor data with high efficiency is used for detection of dengue virus serotypes 2 (DENV-2) and Chikungunya virus (CHIKV) concentration via salphen-Zn (II) and salphen-Cu (II), respectively, in human samples.Design/methodology/approach1H-NMR and 13C-NMR have been used via PerkinElmer LAMBDA 35 instrument. The authors also used a double beam spectrophotometer with (CH3)4Si (TMS) as reference and dimethyl sulfoxide as solvent reference in pH = 7.0. Various DNA concentrations have been used for UV spectrophotometry at 300 nm and 400 nm for zinc and copper complexes, respectively. BRUKER mass spectra with DIONEX Ultimate 3000 LC model were used for all measurements. Mettler Teledo model (DSC882e) of differential scanning calorimeter (DSC) was used for measure the melting temperature of metal zinc and copper complexes. The morphology of the silica Nano spheres (SiNs) were scanned by FESEM with Model JSM-6700F from Japan.FindingsThe Cu (II) and Zn (II)-salphen-viruses DNA system for CHIKV and DENV-2, respectively, in different concentration have been investigated via various spectroscopies (Figure 3). CHIKV and DENV-2 DNA were selected from human saliva and urine samples as models for conformations of human G4-DNA. By increasing the amounts of DNAs, and G4, the UV–Vis bands of located above 300 nm, experienced a hypochromic effect. The Cu2+ complex exhibits selectivity towards the G4, and there is a similar affinity for Zn2+ complex binds to the G4. These results collectively suggest that the Cu2+ complex is stronger than the Zn2+ complex. The authors have found copper (II) and zinc (II) compounds and nucleic acid-complexes are strongly fluorescent molecules in the low energy range, from the visible to the near-infrared. Since the fluorescent emission of Zn (II) and Cu (II) complexes are enhanced by the binding to nucleic acids upon visible light exposure when bound to DNA. These complexes are important as selective fluorescent probes for nucleic acids and to highlight their potential application. UV–vis spectroscopy is an accurate for finding the extent of ligand interaction with DNA and metallic complexes–DNA binding. Generally, the binding of intercalative compounds to DNA can be characterized through a
目的由水杨酸锌和水杨酸铜组成的哌啶侧链功能化N,N′-双亚水杨基亚苯基二胺二阴离子(salphen)能够嵌入DNA的核酸碱基堆积,可作为一种光学DNA杂交检测器。通过在玻璃表面涂覆含有3-氨基丙基三乙氧基硅烷的硅烷偶联剂,将DNA和噻吩连接到玻璃表面,该硅烷偶联剂是为用作高亲和力RNA载体基质而合成的。Schiff碱salphen-zinc(II)和salphen-Cu(II)复合物标记的靶核酸探针使DNA生物传感器的颜色分别变为锌和铜的绿色和红色背景色。本研究旨在表明,高效的DNA生物传感器数据可用于通过salphen-Zn(II)和salphen-Cu(II)分别检测人类样本中登革热2型病毒(DENV-2)和基孔肯雅病毒(CHIKV)的浓度。设计/方法/方法1H NMR和13C-NMR已经通过PerkinElmer LAMBDA 35仪器使用。作者还使用双光束分光光度计,以(CH3)4Si(TMS)为参比,二甲基亚砜为溶剂参比,pH=7.0。不同的DNA浓度已被用于300 nm和400 对于锌和铜配合物分别为nm。所有测量均使用DIONEX Ultimate 3000 LC模型的BRUKER质谱。采用差示扫描量热仪(DSC)的Mettler-Teledo模型(DSC882e)测定了金属锌和铜配合物的熔融温度。通过日本JSM-6700F型FESEM扫描二氧化硅纳米球(SiNs)的形态。发现通过各种光谱法分别研究了不同浓度的CHIKV和DENV-2的Cu(II)和Zn(II)-沙芬病毒DNA系统(图3)。从人唾液和尿液样本中选择CHIKV和DENV-2 DNA作为人G4-DNA构象的模型。通过增加DNA和G4的数量 nm)经历了低变色效应。Cu2+复合物对G4表现出选择性,并且对Zn2+复合物与G4结合具有类似的亲和力。这些结果共同表明Cu2+络合物比Zn2+络合物更强。作者发现,铜(II)和锌(II)化合物以及核酸复合物在从可见光到近红外的低能量范围内都是强荧光分子。由于Zn(II)和Cu(II)复合物的荧光发射在与DNA结合时在可见光暴露时通过与核酸结合而增强。这些复合物作为核酸的选择性荧光探针是重要的,并突出了它们的潜在应用。紫外-可见光谱法可以准确地发现配体与DNA和金属配合物(DNA结合)的相互作用程度。通常,嵌入化合物与DNA的结合可以通过吸收光谱滴定来表征,在这项工作中观察到吸光度降低(低色性)和向更长波长的转移(红移)。独创性/价值血清样本以柠檬酸盐的形式提供,并在血液凝结后收集在试管中。然后,通过离心分离,作者将血清在4°C下冷藏或在-20°C下冷冻。值得注意的是;这些标本已被疾病控制中心登革热分支机构确认。在这项工作中,这些样本之前已经冷冻过,美国疾病控制与预防中心登革热分支机构的诊断实践测试已经在血清和血浆中进行了验证。因此,在薰衣草或肝素中分离的血浆适合并可用于血清学检测。
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