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Filtered back projection-deconvolution method for light field reconstruction from the focal stack 从焦堆重建光场的滤波背投-解卷积法
IF 3.5 2区 工程技术 Q2 OPTICS Pub Date : 2024-09-16 DOI: 10.1016/j.optlaseng.2024.108576

In the simplification, a light field is a four-dimensional (4D) function, and light field reconstruction aims to recover this 4D function from a three-dimensional (3D) focal stack, so it is a seriously ill-posed reconstruction problem from incomplete projection data. Based on the known 3D data of the focal stack in the frequency domain, we introduce a 3D assumption for the light field and derive an analytical reconstruction formula of the light field with an infinite depth range ρ. Subsequently, we establish the filtered back projection (FBP) algorithm to reconstruct the light field from the focal stack. Under certain assumptions concerning the light field and window functions, we prove the convergence of our proposed method at any continuous point. Since in actual data sampling scenarios, the light field is reconstructed only by a small number of focal stacks, a deconvolution algorithm is introduced based on the FBP algorithm to further enhance quality, which is called the filtered back projection-deconvolution (FBP-D) method. Our experimental results demonstrate the superiority of the proposed algorithm compared to the FBP method and other existing methods. Notably, the algorithm exhibits enhanced performance when employing a smooth boundary window and a larger depth range ρ.

在简化过程中,光场是一个四维(4D)函数,而光场重构的目的是从三维(3D)焦堆中恢复这个 4D 函数,因此这是一个从不全投影数据中进行重构的严重问题。基于频域中已知的三维焦堆数据,我们引入了光场的三维假设,并推导出无限深度范围 ρ 的光场解析重建公式。在光场和窗口函数的某些假设条件下,我们证明了我们提出的方法在任何连续点上的收敛性。由于在实际数据采样场景中,光场仅由少量焦堆重建,因此我们在 FBP 算法的基础上引入了一种解卷积算法,以进一步提高质量,这种算法被称为滤波背投-解卷积(FBP-D)方法。我们的实验结果表明,与 FBP 方法和其他现有方法相比,所提出的算法更具优势。值得注意的是,该算法在采用平滑边界窗口和更大深度范围 ρ 时表现出更强的性能。
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引用次数: 0
Field-portable digital holographic quantitative phase imaging with a compact microscope's add-on module 利用紧凑型显微镜的附加模块进行现场便携式数字全息定量相位成像
IF 3.5 2区 工程技术 Q2 OPTICS Pub Date : 2024-09-16 DOI: 10.1016/j.optlaseng.2024.108580

Digital holographic microscopy (DHM) is emerged as a promising quantitative phase-contrast imaging tool for full complex wavefront reconstruction of micron-sized bio-samples. The technique covers the dynamics investigation ranging in scales from sub-cellular to tissue and from milliseconds to hours. Recent advances of DHM lie in the configuration and numerical development of the method and making it more feasible for the users without optical expertise. In this paper, we aim to propose a low-cost and portable add-on module for DHM, which can be mounted on either the ocular or camera port of a conventional microscope and easily turn it to a multi-modal bright-field and DHM imaging tool. The module works based on the off-axis, common-path geometry using a single Fresnel biprism in the detection path of the microscope. This configuration enables a compact and cost-effective solution for point of care applications and in field measurements. The feasibility and efficiency of the device have been confirmed through several morphological investigations on biological specimens and the sub-nanometer phase stability enables the measurement of cell dynamics and phenotypic changes such as motility, growth, differentiation and membrane oscillations.

数字全息显微镜(DHM)是一种前景广阔的定量相位对比成像工具,可用于微米级生物样本的全复杂波面重建。该技术涵盖了从亚细胞到组织、从毫秒到数小时的动态研究。DHM 的最新进展在于该方法的配置和数值开发,并使其对没有光学专业知识的用户更加可行。在本文中,我们旨在提出一种低成本、便携式的 DHM 附加模块,该模块可安装在传统显微镜的目镜或相机端口上,轻松将其转变为多模式明视野和 DHM 成像工具。该模块的工作原理是在显微镜的检测路径上使用单个菲涅尔双棱镜,采用离轴共路径几何结构。这种配置为医疗点应用和现场测量提供了一种紧凑、经济的解决方案。该装置的可行性和效率已通过对生物标本的形态学研究得到证实,亚纳米级的相位稳定性使其能够测量细胞动态和表型变化,如运动、生长、分化和膜振荡。
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引用次数: 0
Polarization-enhanced contrast imaging for pupil detection 用于瞳孔检测的偏振增强对比成像技术
IF 3.5 2区 工程技术 Q2 OPTICS Pub Date : 2024-09-16 DOI: 10.1016/j.optlaseng.2024.108595

In pupil detection within the visible light spectrum, intensity information serves as a carrier for capturing the reflective characteristics of images. When the reflectance of the pupil and its adjacent iris is similar, effectively distinguishing between them becomes challenging. Polarization provides additional information sensitive to the physical and chemical properties of objects, aiding in overcoming this problem. In the polarimetric pupil detection method, the transmission process of polarized light in the human eye model is theoretically analyzed. Arbitrary orthogonal polarization channels are utilized instead of intensity to describe the collected image, facilitating the extraction of polarization information corresponding to each channel. Experimental validation of the proposed method was conducted using active polarization illumination imaging experiments. The experimental results verify that the polarimetric pupil detection method could not only suppress the scatter noise but also be capable of obtaining a combination of intensity and polarization information. Moreover, exploiting the distinctions in depolarization characteristics among biological tissues can substantially improve their contrast.The research findings presented in this article provide insights into enhancing imaging methods for existing pupil detection schemes.

在可见光光谱内的瞳孔检测中,强度信息是捕捉图像反射特性的载体。当瞳孔及其相邻虹膜的反射率相似时,有效区分它们就变得非常困难。偏振提供了对物体物理和化学特性敏感的额外信息,有助于克服这一问题。偏振瞳孔检测方法从理论上分析了偏振光在人眼模型中的传输过程。利用任意正交偏振通道代替强度来描述采集到的图像,便于提取每个通道对应的偏振信息。利用主动偏振照明成像实验对提出的方法进行了实验验证。实验结果验证了偏振瞳孔检测方法不仅能抑制散射噪声,还能获得强度和偏振信息的组合。此外,利用生物组织间去极化特性的差异,可以大大提高它们的对比度。本文介绍的研究成果为改进现有瞳孔检测方案的成像方法提供了启示。
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引用次数: 0
Exploration of moiré tomography in spatial distribution measurement of wind velocity 摩尔层析成像技术在风速空间分布测量中的应用探索
IF 3.5 2区 工程技术 Q2 OPTICS Pub Date : 2024-09-14 DOI: 10.1016/j.optlaseng.2024.108579

In this paper, moiré tomography is applied to measure the spatial velocity distribution of thermal wind field. To verify the feasibility of our method, three sets of experiments are carried out, each lasting for 5 min. And then, its spatial distribution is obtained based on the theoretical relation between the refractive index extracted from moiré fringes and the wind velocity. Finally, some validation is performed for the reliability and accuracy of the method. The radial distribution of wind velocity matches well with those estimated results based on a reported theoretical model. Besides, the general trend of wind velocity values at different times of moiré tomography measurement is almost consistent with those recorded by the anemometer, and the relative error between them maintains within 3 %. In further, some reasonable analysis of the factors contributing to error is provided. In a word, this paper demonstrates that moiré tomography has the potential application to offer a feasible means for the spatial measurement of wind velocity.

本文采用摩尔层析成像技术测量热风场的空间速度分布。为了验证我们方法的可行性,我们进行了三组实验,每组持续 5 分钟。然后,根据从摩尔条纹中提取的折射率与风速之间的理论关系,得出其空间分布。最后,对该方法的可靠性和准确性进行了验证。风速的径向分布与基于理论模型的估计结果非常吻合。此外,摩尔层析成像测量不同时间的风速值的总体趋势与风速计记录的风速值基本一致,两者之间的相对误差保持在 3% 以内。此外,本文还对造成误差的因素进行了合理分析。总之,本文证明了摩尔层析成像技术的潜在应用价值,为风速的空间测量提供了一种可行的方法。
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引用次数: 0
Simulation and experimental analysis of a double-liquid negative lens based on flat electrode 基于平面电极的双液负透镜的仿真与实验分析
IF 3.5 2区 工程技术 Q2 OPTICS Pub Date : 2024-09-14 DOI: 10.1016/j.optlaseng.2024.108594

In order to carry out detailed research on focusing of liquid lens in negative optical power with simple structure, a double-liquid negative lens based on flat electrode is proposed. By adjusting voltage applied to indium tin oxide (ITO) conductive glass plates, the interface between glycerol and silicone oil can be changed to tune the focal length. With COMSOL, MATLAB and Zemax softwares, an optical model of the double-liquid negative lens based on flat electrode is established, its focal length at different voltages is simulated and analyzed, and it is found that when the flat exists with a small tilt (1°∼4°), the parallelism of the flat electrode has little influence on the focal length. In addition, through device fabrication and experimental analysis of this double-liquid negative lens, the focal length of the lens changes from -40.21622 mm to -24.2088 mm when operating voltage is 0V-320 V, which is basically consistent with the simulation results. And the maximum resolution of the proposed liquid lens can reach 71.838lp/mm. A lens with negative focal length holds great promise in diverse applications such as endoscopes, holographic imaging and adaptive optics.

为了对结构简单的负光学功率液体透镜聚焦进行详细研究,提出了一种基于平面电极的双液负透镜。通过调节施加在铟锡氧化物(ITO)导电玻璃板上的电压,可以改变甘油和硅油之间的界面,从而调节焦距。利用 COMSOL、MATLAB 和 Zemax 软件,建立了基于平板电极的双液负透镜光学模型,模拟并分析了其在不同电压下的焦距,发现当平板存在较小倾斜度(1°∼4°)时,平板电极的平行度对焦距的影响很小。此外,通过该双液负透镜的器件制作和实验分析,当工作电压为 0V-320 V 时,透镜的焦距从 -40.21622 mm 变为 -24.2088 mm,与模拟结果基本一致。而所提出的液体透镜的最大分辨率可达 71.838lp/mm。负焦距透镜在内窥镜、全息成像和自适应光学等多种应用中大有可为。
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引用次数: 0
Active illumination mode with checkerboard pattern in focus variation microscopy: Analysis and application 聚焦变化显微镜中的棋盘格图案主动照明模式:分析与应用
IF 3.5 2区 工程技术 Q2 OPTICS Pub Date : 2024-09-14 DOI: 10.1016/j.optlaseng.2024.108584

Optical measurement methods for surface topography offer the advantages of high accuracy, rapid measurement, and non-destructiveness. Each method has its own suitable application scenarios. Among them, focus variation microscopy is extensively employed in precision manufacturing, aerospace, and medical industries due to its ability to measure rough and large slopes surfaces. However, since the measurement depends on local grayscale differences between focused and blurred images, it cannot measure surfaces with low reflectivity or insufficient texture information. In this work, we propose an active illumination mode for focus variation method that utilizes a digital micromirror device (DMD) to generate a checkerboard pattern. This method introduces additional texture information, resulting in a usable local gradient of image grayscale. Additionally, we analyze the selection criteria for the checkerboard pattern parameters, including the period and light-dark ratio. Furthermore, measurements of two standard steps with different heights demonstrate that the measurement repeatability of the proposed method can reach the nanometer level, rendering it suitable for high-precision measurements. More importantly, the measurement noise results indicate significantly superior performance of active illumination mode compared to the uniform illumination mode. Finally, we reconstruct the surface topography of the microchannels in a microfluidic chip through the encapsulation layer, demonstrating the feasibility of the proposed method.

光学测量表面形貌的方法具有精度高、测量速度快和无损伤等优点。每种方法都有其适合的应用场合。其中,聚焦变化显微镜因其能够测量粗糙和大斜度表面而被广泛应用于精密制造、航空航天和医疗行业。然而,由于测量依赖于聚焦图像和模糊图像之间的局部灰度差异,它无法测量低反射率或纹理信息不足的表面。在这项工作中,我们提出了一种主动照明模式的聚焦变化方法,它利用数字微镜设备(DMD)生成棋盘图案。这种方法引入了额外的纹理信息,从而产生了可用的图像灰度局部梯度。此外,我们还分析了棋盘图案参数的选择标准,包括周期和明暗比。此外,对两个不同高度的标准台阶的测量结果表明,所提出方法的测量重复性可以达到纳米级水平,因此适用于高精度测量。更重要的是,测量噪声结果表明,主动照明模式的性能明显优于均匀照明模式。最后,我们通过封装层重建了微流控芯片中微通道的表面形貌,证明了所提方法的可行性。
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引用次数: 0
Chaos and bifurcation in the vibration of a metal cantilever excited by a modulated pulsed laser 受调制脉冲激光激励的金属悬臂振动中的混沌和分叉现象
IF 3.5 2区 工程技术 Q2 OPTICS Pub Date : 2024-09-14 DOI: 10.1016/j.optlaseng.2024.108593

Laser-induced vibration is a promising principle of actuation for its energy conversion from optical to mechanical. The nonlinearity of the optical-thermal-mechanical coupling leads to a narrow drive bandwidth in the high vibration mode and limits its applications to conventional micrometer level. In this study, a mathematical model coupling the photothermal effect and the thermoelastic effect has been derived. The numerical calculation shows that the vibration of the cantilever exhibits chaos, bifurcation and modal interaction as the modulated frequency changes, indicating the potential excitation strategy that we can take advantage of these nonlinear states to enhance the energy efficiency beyond micrometer-scale actuation. We propose a new excitation method to enhance the energy efficiency enabling the generation of millimeter-scale vibrations with a single-point pulsed laser. The nonlinearity of cantilever vibration can be further enhanced by controlling the pulse laser frequency, driving the system from stable state to chaos and bifurcation, which leads to increased amplitude and energy efficiency. Compared to stable state, chaos and bifurcation can amplify the amplitude of the cantilever by 5 to 10 times, respectively, with a maximum amplitude of 0.69 mm and 2.31 mm in experimental validations. This allows the laser induced excitation to offer the potential for widely using in non-destructive testing, precision operations, and driving micro-resonators.

激光诱导振动是从光学到机械的能量转换,是一种很有前途的驱动原理。光-热-机械耦合的非线性导致高振动模式的驱动带宽较窄,限制了其在传统微米级的应用。在这项研究中,推导出了一个将光热效应和热弹性效应耦合在一起的数学模型。数值计算表明,随着调制频率的变化,悬臂的振动表现出混沌、分岔和模态交互作用,这表明我们可以利用这些非线性状态来提高微米级致动的能量效率。我们提出了一种新的激励方法来提高能量效率,从而利用单点脉冲激光产生毫米级振动。通过控制脉冲激光频率,可进一步增强悬臂振动的非线性,使系统从稳定状态进入混沌和分叉状态,从而提高振幅和能量效率。与稳定状态相比,混沌和分叉可分别将悬臂的振幅放大 5 至 10 倍,实验验证的最大振幅分别为 0.69 毫米和 2.31 毫米。这使得激光诱导激励有望广泛应用于无损检测、精密操作和驱动微谐振器。
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引用次数: 0
Systematical and universal calibration scheme for division-of-aperture polarimetric camera 分光圈偏振照相机的系统性通用校准方案
IF 3.5 2区 工程技术 Q2 OPTICS Pub Date : 2024-09-13 DOI: 10.1016/j.optlaseng.2024.108589

Division-of-aperture polarimetric (DoAP) camera has been widely used to obtain polarization images of scene owing to its feature in simultaneous acquisition of polarization information, real-time measurement and compact system. However, practical applications of DoAP imaging inevitably involve calibrating the camera from several aspects, which is very essential but the related reports are relatively lacking in current research. In this paper, we proposed a systematical and universal calibration scheme for the DoAP camera, which includes the lens distortion correction, the image registration, the polarization channel calibration and the Stokes parameters modification. Accordingly, a software for calibration and imaging processing, which consists of four modules, is designed to collectively facilitate the efficient and accurate execution of all calibration steps. In order to achieve real-time polarimetric imaging, the lens distortion correction and the image registration were done by means of lookup tables. Experimental results by using a full-Stokes DoAP camera we fabricated indicate that, via the lens distortion correction and the image registration, the alignment error between two images with a mean absolute error (MAE) less than 0.16 pixels can be obtained, resulting in the aligned images having an average increase of 64.53 % for the structural similarity index (SSIM) and that of 84.97 % for the peak signal to noise ratio (PSNR), respectively; and further via the polarization channel calibration and the Stokes parameters modification, the MAE of 0.0146 for the degree of polarization (DoP) and that of 0.3544° for the angle of polarization (AoP) are obtained, respectively. This proposed calibration scheme for DoAP camera is systematic, automatic and robust, which is benefit of improving the polarization detection accuracy, enhancing the camera calibration efficiency and thus promoting its polarimetric imaging performance.

分光圈偏振(DoAP)相机具有同时获取偏振信息、实时测量和系统紧凑等特点,已被广泛用于获取场景的偏振图像。然而,在 DoAP 成像的实际应用中,不可避免地要从多个方面对相机进行校准,这一点非常必要,但目前相关研究报告相对缺乏。本文提出了一种系统而通用的 DoAP 相机校准方案,包括镜头畸变校正、图像配准、偏振通道校准和斯托克斯参数修正。因此,我们设计了一个由四个模块组成的校准和成像处理软件,以共同促进所有校准步骤的高效和准确执行。为了实现实时偏振成像,通过查找表完成了镜头畸变校正和图像配准。使用我们制造的全斯托克斯 DoAP 相机进行的实验结果表明,通过镜头畸变校正和图像配准,可以获得平均绝对误差(MAE)小于 0.16 像素的两幅图像之间的配准误差,从而使配准图像的结构相似度平均提高 64.此外,通过偏振通道校准和斯托克斯参数修正,偏振度(DoP)的平均绝对误差(MAE)为 0.0146,偏振角(AoP)的平均绝对误差(MAE)为 0.3544°。这种针对 DoAP 相机提出的校准方案具有系统性、自动性和鲁棒性,有利于提高偏振检测精度,提高相机校准效率,从而提升其偏振成像性能。
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引用次数: 0
Transformer based deep learning for digital image correlation 基于变压器的数字图像关联深度学习
IF 3.5 2区 工程技术 Q2 OPTICS Pub Date : 2024-09-13 DOI: 10.1016/j.optlaseng.2024.108568

Deep learning is expected to overcome the intractable challenges that traditional digital image correlation (DIC) confronts in the measurement of extremely complex deformation fields with severe spatial variation. The recently developed DIC methods based on convolutional neural network (CNN) achieved notable success in tackling highly localized deformation. This paper introduces an innovative DIC method based on Transformer (named DICTr), which is found to outperform its CNN-based counterparts. Different from CNN-based DIC that try to establish the relationship between the changes in grayscale values and the displacements through regression, DICTr reformulates the problem back to the image registration driven by feature matching. The displacement field is estimated in a coarse-to-fine way through matching the features enhanced by a sophisticatedly designed Transformer encoder. In experimental verification, DICTr demonstrates outstanding accuracy and robustness to measure complex displacement fields, and balances well the spatial resolution and measurement resolution.

深度学习有望克服传统数字图像相关(DIC)在测量具有严重空间变化的极其复杂的形变场时所面临的棘手难题。最近开发的基于卷积神经网络(CNN)的 DIC 方法在处理高度局部化的形变方面取得了显著成功。本文介绍了一种基于变换器的创新 DIC 方法(命名为 DICTr),发现该方法优于基于 CNN 的同类方法。基于 CNN 的 DIC 尝试通过回归建立灰度值变化与位移之间的关系,而 DICTr 则不同,它将问题重新归结为由特征匹配驱动的图像注册。通过匹配经复杂设计的变换器编码器增强的特征,以从粗到细的方式估算位移场。在实验验证中,DICTr 在测量复杂位移场方面表现出卓越的准确性和鲁棒性,并很好地平衡了空间分辨率和测量分辨率。
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引用次数: 0
Simultaneous estimation of multiple order phase derivatives using deep learning method in digital holographic interferometry 在数字全息干涉测量中使用深度学习方法同时估算多阶相位导数
IF 3.5 2区 工程技术 Q2 OPTICS Pub Date : 2024-09-13 DOI: 10.1016/j.optlaseng.2024.108583

For non-contact deformation testing, digital holographic interferometry is a prominent optical technique where the first and second order interference phase derivatives directly embed information about the strain and curvature distributions of a deformed object. Hence, reliable extraction of multiple order phase derivatives is of great practical significance; however, this problem is marred by several challenges such as the need of multiple differentiation operations, complex shearing operations and performance degradation due to noise. In this paper, we introduce a deep learning approach for the direct and simultaneous estimation of first and second order phase derivatives in digital holographic interferometry. Our method's performance is demonstrated via rigorous numerical simulations exhibiting wide range of additive white Gaussian noise and speckle noise. Moreover, we substantiate the practical efficacy of our proposed method for processing deformation fringes acquired via digital holographic interferometry.

数字全息干涉测量法是一种突出的光学技术,其一阶和二阶干涉相位导数直接嵌入变形物体的应变和曲率分布信息,用于非接触变形测试。因此,可靠地提取多阶相位导数具有重要的现实意义;然而,这一问题面临着诸多挑战,如需要进行多次微分操作、复杂的剪切操作以及噪声导致的性能下降。在本文中,我们介绍了一种深度学习方法,用于直接同时估计数字全息干涉测量中的一阶和二阶相位导数。我们的方法通过严格的数值模拟证明了其性能,并展示了广泛的加性白高斯噪声和斑点噪声。此外,我们还证实了我们提出的方法在处理通过数字全息干涉测量获得的形变条纹方面的实际功效。
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引用次数: 0
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Optics and Lasers in Engineering
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