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Effects of heavy metal contamination on fungal diversity in Pinus brutia shoots 重金属污染对欧洲赤松嫩枝真菌多样性的影响
Pub Date : 2024-03-13 DOI: 10.15376/biores.19.2.2724-2735
Melike Cebi Kilicoglu
The effects of heavy metal pollution have become a significant global issue in recent years. The primary objective of the present study was to compare the heavy metal concentrations in Pinus brutia shoots grown in an organized industrial zone (OIZ) and a forested area (Adalar) and to examine how these heavy metals affect fungal microbiota. The results achieved here showed that Ni and V concentrations were lower than the detectable limits in both the Adalar and the OIZ region, whereas Se and Cu concentrations were lower than the detectable limits in the shoots collected from the Adalar. Concentrations determined in samples collected from the OIZ were approximately 6 times higher for Cr and 16 times higher for Zn in comparison to the samples collected from the Adalar. Metagenomic analysis revealed that the most common fungal genera were Aureobasidium, Gibberella, Hazslinszkyomyces, Alternaria, Cladosporium, Buckleyzyma, Lasiodiplodia, and Hormonema for the OIZ area and Hormonema, Aureobasidium, Alternaria, Cladosporium, Arthrinium, Fonsecazyma, and Truncatella for the Adalar region. In the future, this study may serve as a reference for the development of innovative strategies for the remediation of heavy metal pollution for a sustainable and clean environment using biological sources.
近年来,重金属污染的影响已成为一个重要的全球性问题。本研究的主要目的是比较生长在有组织工业区(OIZ)和森林地区(Adalar)的欧洲赤松嫩枝中的重金属浓度,并研究这些重金属如何影响真菌微生物群。研究结果表明,在 Adalar 和 OIZ 地区,镍和钒的浓度均低于可检测到的限值,而在 Adalar 采集的嫩枝中,硒和铜的浓度低于可检测到的限值。与从阿达拉尔采集的样本相比,从 OIZ 采集的样本中测定的铬和锌浓度分别高出约 6 倍和 16 倍。元基因组分析表明,在 OIZ 地区最常见的真菌属是 Aureobasidium、Gibberella、Hazslinszkyomyces、Alternaria、Cladosporium、Buckleyzyma、Lasiodiplodia 和 Hormonema;在 Adalar 地区最常见的真菌属是 Hormonema、Aureobasidium、Alternaria、Cladosporium、Arthrinium、Fonsecazyma 和 Truncatella。今后,这项研究可作为制定创新战略的参考,以便利用生物资源修复重金属污染,实现可持续的清洁环境。
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引用次数: 0
In vitro and molecular docking evaluation of target proteins of lipase and protease for the degradation of aflatoxins 脂肪酶和蛋白酶降解黄曲霉毒素目标蛋白的体外和分子对接评估
Pub Date : 2024-03-13 DOI: 10.15376/biores.19.2.2701-2713
A. M. Al-Rajhi, M. Ganash, Amal Naif Alshammari, Sulaiman A. Alsalamah, Tarek M. Abdelghany
The consumption of food contaminated with aflatoxins causes severe harmful health effects, which can lead to death, in both humans and livestock. Therefore, the degradation of aflatoxins, particularly by biological methods, is considered a feasible technology for remediation of aflatoxin-contaminated products. In vitro, aflatoxin B1, B2, G1, and G2 were degraded by 25 U/mL of lipase with reduction percentages of 57.5, 71.7, 80.1, and 83.8%. This reduction increased to 81.3, 82.8, 86.9, and 90.7% via 200 U/mL of lipase, respectively. Protease was less effective than lipase in the degradation of aflatoxin B1, B2, G1, and G2 with reduction levels of 35.8, 54.9, 66.5, and 70.2%, respectively, at 25 U/mL of protease. This investigation offers new concepts for the quick screening of aflatoxin-degrading enzymes and offers a theoretical basis for the degradation of aflatoxins. Interactions between aflatoxin B1 (considered as a ligand) and proteins that were taken as receptors (Structure of Lipase PDB ID: 1DT3 and Protease PDB ID: 2PRO) were elucidated. The molecular modeling results of utilized compound showed a notable binding score and best Root Mean Square (RMS) define value, indicating efficient binding mode and appropriate interactions with amino acids of selected proteins.
食用受黄曲霉毒素污染的食品会对人类和牲畜的健康造成严重危害,甚至导致死亡。因此,降解黄曲霉毒素,特别是采用生物方法降解黄曲霉毒素,被认为是对受黄曲霉毒素污染的产品进行补救的可行技术。在体外,黄曲霉毒素 B1、B2、G1 和 G2 被 25 U/mL 脂肪酶降解,降解率分别为 57.5%、71.7%、80.1% 和 83.8%。使用 200 U U/mL 的脂肪酶后,降解率分别增至 81.3%、82.8%、86.9% 和 90.7%。蛋白酶对黄曲霉毒素 B1、B2、G1 和 G2 的降解效果不如脂肪酶,在蛋白酶 25 U U/mL 的作用下,黄曲霉毒素 B1、B2、G1 和 G2 的降解率分别为 35.8%、54.9%、66.5% 和 70.2%。这项研究为快速筛选黄曲霉毒素降解酶提供了新的概念,并为黄曲霉毒素的降解提供了理论依据。研究阐明了黄曲霉毒素 B1(作为配体)与作为受体的蛋白质(脂肪酶结构 PDB ID:1DT3 和蛋白酶结构 PDB ID:2PRO)之间的相互作用。所利用化合物的分子建模结果显示了显著的结合得分和最佳均方根(RMS)定义值,这表明了有效的结合模式以及与所选蛋白质氨基酸的适当相互作用。
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引用次数: 0
Effect of surfactant on pseudo-lignin formation 表面活性剂对假木质素形成的影响
Pub Date : 2024-03-13 DOI: 10.15376/biores.19.2.2714-2723
Zhoubo Yao, Yayu Liang, Peng Zhan, Lishu Shao, Zhiping Wu
In this study, a nonionic surfactant (JFC-M) was used as an additive for hydrothermal pretreatment of crushed poplar wood. The pseudo-lignin extracted from holocellulose after hydrothermal pretreatment was characterized, and the composition of liquid and solid fractions obtained after pretreatment at different experimental conditions was analyzed. The results showed that the addition of JFC-M surfactant accelerated the dissolution of biomass cellulose and effectively inhibited the production of pseudo-lignin in hydrothermal processes, under the same hydrothermal pretreatment conditions. The pseudo-lignin yield for the control group was 14.2% (no JFC surfactant added), whereas when the JFC-M concentration was 2%, the pseudo-lignin yield was 9.8%.
本研究使用了一种非离子表面活性剂(JFC-M)作为添加剂,对粉碎的杨木进行水热预处理。对水热预处理后从全纤维素中提取的假木质素进行了表征,并分析了不同实验条件下预处理后得到的液体和固体馏分的成分。结果表明,在相同的水热预处理条件下,添加 JFC-M 表面活性剂可加速生物质纤维素的溶解,并有效抑制水热过程中假木质素的生成。对照组的假木质素产量为 14.2%(未添加 JFC 表面活性剂),而当 JFC-M 浓度为 2% 时,假木质素产量为 9.8%。
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引用次数: 0
The compostability of denim fabrics dyed with various indigos 用各种靛蓝染色的牛仔面料的可堆肥性
Pub Date : 2024-03-11 DOI: 10.15376/biores.19.2.2685-2700
Wendy Alwala, Antonia Perju, M. Schwarz, Jean Bonhotal, Steven Pires, Mary A. Ankeny, Jesse Daystar, Margaret Frey
Denim fabric samples representing current indigo dye sources and fabric structures were biodegraded in feedstock including food waste, manure, and animal bedding, which are typically composted at the Cornell Farm Services Composting Facility and processed under laboratory conditions for 77 days. Indigo types including dry denim, pre-reduced, and natural did not inhibit degradation as compared to undyed 100% cotton fabric. Additionally, fabrics tested as received from the mill and those tested post scouring degraded effectively. As expected, denim containing 24% polyester and 2% spandex retained overall fabric structure despite degradation of the cotton portion of the yarns.
代表当前靛蓝染料来源和织物结构的牛仔面料样品在包括食物垃圾、粪便和动物垫料在内的原料中进行了生物降解,这些原料通常在康奈尔农场服务堆肥设施中堆肥,并在实验室条件下处理 77 天。与未染色的 100% 棉织物相比,包括干牛仔布、预还原布和天然靛蓝在内的靛蓝类型并不能抑制降解。此外,从纺织厂收到的测试织物和经过冲洗后的测试织物都能有效降解。正如预期的那样,含有 24% 聚酯和 2% 氨纶的牛仔布尽管纱线中的棉部分发生了降解,但仍能保持织物的整体结构。
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引用次数: 0
Development and characterization of nanocellulose/ carbonized waste rubber nanocomposites 纳米纤维素/碳化废橡胶纳米复合材料的开发与表征
Pub Date : 2024-03-08 DOI: 10.15376/biores.19.2.2670-2684
Engin Kocatürk, Ferhat Şen, Mustafa Zor, Zeki Candan
Recycling is one of the most popular research topics today. In this study, in addition to the evaluation of waste tires, which are frequently encountered in the industry and difficult to dispose of, a green biomaterial, nanocellulose-based new generation nanocomposite was produced and characterized for the first time. Carbonized waste rubber, obtained by pyrolysis of tire wastes, was reinforced with nanocellulose at levels of 0.10%, 0.25%, 0.5%, and 1% by weight. The prepared nanocellulose-based nanocomposites were investigated by X-ray diffraction (XRD), morphological properties by scanning electron microscopy (SEM), thermal properties by thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and dynamic mechanical thermal (DMTA). In addition, the percentage of gel contents of the produced nanocomposites were determined. Thermal analyses revealed that the sample containing 1% carbonized waste rubber showed the highest thermal stability and at 750 °C the ash yield increased up to 25% compared to nanocellulose. The fabricated nanocomposites had about 10 times higher storage modulus compared to pure NC. All results show that the green nanocellulose-based nanocomposites can be used for future applications in industry.
回收利用是当今最热门的研究课题之一。在本研究中,除了对工业中经常遇到且难以处理的废轮胎进行评估外,还首次生产了一种绿色生物材料,即基于纳米纤维素的新一代纳米复合材料,并对其进行了表征。通过热解轮胎废料得到的碳化废橡胶中添加了纳米纤维素,添加量分别为 0.10%、0.25%、0.5% 和 1%。通过 X 射线衍射(XRD)、扫描电子显微镜(SEM)、热重分析(TGA)、差示扫描量热仪(DSC)和动态机械热(DMTA)研究了所制备的纳米纤维素基纳米复合材料的形态特性。此外,还测定了所制纳米复合材料的凝胶含量百分比。热分析表明,含 1%碳化废橡胶的样品显示出最高的热稳定性,与纳米纤维素相比,在 750 °C 时,灰分产量增加了 25%。与纯 NC 相比,制备的纳米复合材料的存储模量高出约 10 倍。所有结果表明,基于纳米纤维素的绿色纳米复合材料可用于未来的工业应用。
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引用次数: 0
Effect of selective enhancement on the bending performance of fused deposition methods 3D-printed PLA models 选择性增强对熔融沉积法三维打印聚乳酸模型弯曲性能的影响
Pub Date : 2024-03-07 DOI: 10.15376/biores.19.2.2660-2669
Chen Wang, Jiahao Yu, Minhan Jiang, Jingyao Li
The top and bottom shells of fused deposition 3D-printed PLA models are exposed to the highest stresses. To improve the bending performance of PLA models under three-point bending conditions, the models were strengthened by a selective enhancement method. Several sets of PLA models were fabricated using FDM technology, and three-point bending experiments were conducted to compare the bending strength of PLA models when the layer height, top/bottom shell thickness, and extrusion rate were varied. The bending strength of the PLA models increased as the layer height of the top/bottom shell decreased, the thickness increased, and the extrusion rate increased. The average bending strength of the PLA models after selective enhancement was 84.4 MPa, and the average bending modulus of elasticity was 0.816 GPa, which were higher than the average bending strength of 68.6 MPa and the average bending modulus of elasticity of 0.736 GPa of the conventional groups. These results indicated that the selective enhancement method improved the bending performance of 3D-printed PLA models, and it also provided a reference for the improvement of the mechanical properties of the 3D-printed models with cellulose composite reinforced materials.
熔融沉积 3D 打印聚乳酸模型的顶部和底部外壳承受的应力最大。为了改善聚乳酸模型在三点弯曲条件下的弯曲性能,采用选择性增强方法对模型进行了增强。利用 FDM 技术制作了多组聚乳酸模型,并进行了三点弯曲实验,比较了聚乳酸模型在层高、顶/底壳厚度和挤出速率变化时的弯曲强度。聚乳酸模型的弯曲强度随着顶/底壳层高的降低、厚度的增加和挤出速率的提高而增加。选择性增强后聚乳酸模型的平均弯曲强度为 84.4 MPa,平均弯曲弹性模量为 0.816 GPa,均高于传统组的平均弯曲强度 68.6 MPa 和平均弯曲弹性模量 0.736 GPa。这些结果表明,选择性增强方法改善了三维打印聚乳酸模型的弯曲性能,同时也为纤维素复合增强材料三维打印模型力学性能的改善提供了参考。
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引用次数: 0
Influence of washing with sodium lauryl sulphate (SLS) surfactant on different properties of ramie fibres 用十二烷基硫酸钠(SLS)表面活性剂洗涤对苎麻纤维不同特性的影响
Pub Date : 2024-03-06 DOI: 10.15376/biores.19.2.2609-2625
Murugesan Palaniappan, Sivasubramanian Palanisamy, Thulasi Mani Murugesan, Srinivas Tadepalli, Rashid Khan, Sabbah Ataya, Carlo Santulli
Green composite materials are a means of reducing reliance on synthetic and especially single-use plastics (SUP) and raising public awareness of the need for urgent action to protect the planet. Natural (lignocellulosic) fibres are increasingly utilized as the reinforcement in polymer matrix composites, in search for increased renewability and sustainability. This work concerns the effect of washing ramie (Boehmeria nivea) fibres using sodium lauryl sulphate (SLS) surfactant. The SLS-treated ramie fibres were examined for their morphological, physical, thermal, structural, and mechanical properties by powder X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile testing. SLS treated ramie fibres density and crystallinity index values were 1.23 g/cc and 84.5%, respectively, with a very high cellulose content of 81.3%, because hemicellulose and loose particles were dissolved. SEM images depicted the relevant changes, with no significant damage on treated fibre surfaces. With some assistance from the treatment, fibres initiated their degradation only above 250 °C, culminating at 327 °C, which appears suitable for the manufacturing of composites with the most common matrices.
绿色复合材料是减少对合成塑料,特别是一次性塑料(SUP)的依赖,提高公众对采取紧急行动保护地球必要性的认识的一种手段。为了提高可再生性和可持续性,越来越多地使用天然(木质纤维素)纤维作为聚合物基复合材料的增强材料。这项研究涉及使用十二烷基硫酸钠(SLS)表面活性剂洗涤苎麻(苧麻)纤维的效果。通过粉末 X 射线衍射 (XRD)、傅立叶变换红外光谱 (FTIR)、热重分析 (TGA)、扫描电子显微镜 (SEM) 和拉伸测试,检测了经 SLS 处理的苎麻纤维的形态、物理、热、结构和机械性能。经 SLS 处理的苎麻纤维密度和结晶度指数值分别为 1.23 g/cc 和 84.5%,纤维素含量高达 81.3%,这是因为半纤维素和松散颗粒被溶解了。扫描电镜图像显示了相关的变化,处理过的纤维表面没有明显的损伤。在处理方法的帮助下,纤维仅在 250 °C 以上才开始降解,最终降解温度为 327 °C。
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引用次数: 0
Mechanical properties of glulam moment-resisting joints reinforced by inclined self-tapping screws 用倾斜自攻螺钉加固的胶合木抗矩缝的力学性能
Pub Date : 2024-03-06 DOI: 10.15376/biores.19.2.2626-2638
Shuo Wang, Panpan Ma, Jingkang Lin, Tongyu Hou, Fei-bin Wang, Z. Que, Meng Gong
Self-tapping screws (STS) are an effective fastener to enhance wooden moment-resisting joints. However, the effects of the arrangement and insertion angle of STS on the mechanical properties of wooden joints are less studied. Therefore, this study investigated the influence of these two factors on the mechanical properties of wooden joints by conducting cyclic loading tests using glulam moment-resisting joints reinforced by STS with different arrangements (round and square) and insertion angles (45° and 90°). The failure modes, bearing performances, and energy dissipation capacities were considered. The results showed that the insertion angle affected the bearing and energy dissipation capacity of the joints significantly, while the effect of arrangement was slight. The anti-rotation bending moments of the joints reinforced by inclined STS were higher by 31.7% and 13.5% when the arrangement of STS was circular and rectangular respectively compared with the joints reinforced by vertical STS under compression state, and were lower by 17.5 % and 22.9 % under tensile state. The restoring force characteristics of the joints were similar when the insertion angle of STS was the same. Furthermore, the joints had optimal ductility and stiffness when the arrangement was rectangular, and the insertion angle was 45°.
自攻螺钉(STS)是一种有效的紧固件,可增强木制接缝的抗弯矩能力。然而,有关自攻螺钉的排列和插入角度对木制接头机械性能影响的研究较少。因此,本研究使用不同排列方式(圆形和方形)和插入角度(45° 和 90°)的 STS 加固胶合木矩形抗力接头,通过循环加载试验研究了这两个因素对木制接头机械性能的影响。试验考虑了失效模式、承载性能和耗能能力。结果表明,插入角对接头的承载能力和消能能力影响很大,而排列方式的影响很小。与垂直 STS 加固的接头相比,当 STS 的布置为圆形和矩形时,倾斜 STS 加固的接头在压缩状态下的抗旋转弯矩分别提高了 31.7% 和 13.5%,而在拉伸状态下则分别降低了 17.5% 和 22.9%。当 STS 插入角度相同时,接头的恢复力特性相似。此外,当排列方式为矩形、插入角度为 45°时,接头的延展性和刚度均达到最佳状态。
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引用次数: 0
Consumer behavior based on the SOR model: How do short video advertisements affect furniture consumers’ purchase intentions? 基于 SOR 模型的消费者行为:短视频广告如何影响家具消费者的购买意向?
Pub Date : 2024-03-06 DOI: 10.15376/biores.19.2.2639-2659
Shulan Yu, Hongli Zhang, Qun Zheng, Dongdong Chu, Tianyue Chen, Xinran Chen
Short video advertisements are a novel and influential medium for promoting furniture products, but their effects on consumers’ purchase intentions remain underexplored. This study applies the extended stimulus-organism-response (SOR) framework, a psychological theory that elucidates how stimuli (short video ads), organisms (consumers), and responses (purchase intentions) are interrelated. This study quantitatively examines these relationships using structural equation modeling (SEM). The results reveal that the Flow experience and Telepresence experience significantly affect purchase intentions, indicating that consumers who experience high levels of engagement and immersion while viewing short furniture-related video ads are more likely to exhibit buying behavior. This study also identifies three critical antecedents of the Telepresence experience: social influence, perceived entertainment value, and perceived interactivity. These factors may enhance the effectiveness of short-form video advertising by increasing consumer interaction and engagement. Moreover, convenience conditions, perceived entertainment value, and media richness significantly influence consumers’ flow experience. This suggests that these factors should be considered when designing short video advertisements to optimize consumers’ flow experience and thus increase purchase intentions. This study provides empirical evidence for the SOR framework, investigates the impact of short video advertisements on furniture consumers’ purchase intention, and offers practical implications and recommendations for marketing practitioners.
短视频广告是推广家具产品的一种新颖而有影响力的媒介,但其对消费者购买意向的影响仍未得到充分探讨。本研究采用了扩展的刺激-有机体-反应(SOR)框架,这一心理学理论阐明了刺激(短视频广告)、有机体(消费者)和反应(购买意向)之间的相互关系。本研究采用结构方程模型(SEM)对这些关系进行了定量研究。研究结果表明,"流动体验 "和 "网真体验 "对购买意向有显著影响,表明在观看与家具相关的短视频广告时体验到高度参与和沉浸感的消费者更有可能表现出购买行为。本研究还确定了网真体验的三个关键前因:社会影响力、感知娱乐价值和感知互动性。这些因素可以通过增加消费者的互动和参与来提高短视频广告的效果。此外,便利条件、感知娱乐价值和媒体丰富性也会显著影响消费者的流动体验。这表明,在设计短视频广告时应考虑这些因素,以优化消费者的流动体验,从而提高购买意向。本研究为 SOR 框架提供了实证证据,探讨了短视频广告对家具消费者购买意向的影响,并为营销从业者提供了实践意义和建议。
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引用次数: 0
Effects of bleaching treatment on the properties of bio-polyurethane films from liquefied bamboo 漂白处理对液化竹生物聚氨酯薄膜性能的影响
Pub Date : 2024-03-04 DOI: 10.15376/biores.19.2.2562-2574
Redzuan Mohammad Suffian James, P. Tahir, Norwahyuni Mohd Yusof, Syeed Saifulazry Osman Al-Edrus, Zurina Zainal Abidin, Ismawati Palle, M. K. A. Uyup, Seng Hua Lee
Malaysian bamboo residues were subjected to a liquefaction process. Bleaching of the liquefied product was carried out to reduce its coloration. Polyurethane (PU) films were then manufactured as a coating material by reacting the -OH groups in the bamboo material with isocyanate (-NCO). The study’s objective was to investigate the effects of bleaching on the properties of resulting polyurethane films and to elucidate its behavior as affected by the NCO/OH ratios. Bamboo residues, in powder form, were liquefied with polyethylene glycol (PEG 400) and glycerol (Gly) as reactive co-solvent, and sulphuric acid as catalyst. The obtained liquefied bamboo was then bleached with hydrogen peroxide. Bleached and unbleached liquefied bamboo were used to produce PU film by reacting isocyanate at NCO/OH ratios of 1.6, 1.8, 2.0, and 2.2. The results revealed that the NCO/OH ratios improved the mechanical performance of the PU films. Bleaching treatment slightly reduced the thermal stability and mechanical strength of the PU films. However, bleached PU films displayed lower water absorption and lower biodegradation than unbleached PU films. Nevertheless, the performance of the bleached PU films was still acceptable, indicating that the bleaching treatment using hydrogen peroxide is feasible to obtain semi-transparent film.
马来西亚竹渣经过液化处理。对液化产品进行漂白,以减少其色度。然后,通过使竹子材料中的 -OH 基团与异氰酸酯(-NCO)反应,制造出聚氨酯(PU)薄膜作为涂层材料。研究的目的是调查漂白对聚氨酯薄膜性能的影响,并阐明其行为受 NCO/OH 比率的影响。以聚乙二醇(PEG 400)和甘油(Gly)为反应性助溶剂,以硫酸为催化剂,对粉末状竹渣进行液化。然后用过氧化氢对液化竹材进行漂白。将漂白和未漂白的液化竹材用于生产聚氨酯薄膜,以 1.6、1.8、2.0 和 2.2 的 NCO/OH 比率与异氰酸酯反应。结果表明,NCO/OH 比提高了聚氨酯薄膜的机械性能。漂白处理略微降低了聚氨酯薄膜的热稳定性和机械强度。不过,与未漂白聚氨酯薄膜相比,漂白聚氨酯薄膜的吸水性和生物降解性更低。尽管如此,漂白聚氨酯薄膜的性能仍然可以接受,这表明使用双氧水进行漂白处理以获得半透明薄膜是可行的。
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引用次数: 0
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