S. Purushothaman, A. A. Al bshabshe, Alwadai Nasser Mohammed M., Naif Sulaiman, Omprakash Palanivel
Death is an assured inevitability event for all living beings of this cosmos. Based on the American Academy of Neurology (AAN) recommendations, clinical diagnosis of Brain Death (BD) includes multiple sequences of tests. The Apnea Test (AT) is a more significant procedure in diagnosing BD. Unfortunately, complications during this procedure can occur more frequently than documented, and its incidence is limited to case reports. However, most critical care physicians and Cardio-respiratory therapists consider an AT a principal method to confirm BD as it provides decisive information about the definitive loss of brainstem function. Here, we describe the execution of the AT procedure that transpired into extensive subcutaneous emphysema, tension pneumothorax, and pneumoperitoneum followed by pulseless electrical activity due to direct airway perforation by the supplemental oxygen catheter. In addition, we do a literature review on airway complications incidence and suggest methods to avoid such fatal complications. Finally, further prospective studies are recommended to evaluate the barotrauma incidence and complication nature during AT.
{"title":"Apnea test induced barotrauma in an obese subject with a short neck - A case report","authors":"S. Purushothaman, A. A. Al bshabshe, Alwadai Nasser Mohammed M., Naif Sulaiman, Omprakash Palanivel","doi":"10.51248/.v43i3.2224","DOIUrl":"https://doi.org/10.51248/.v43i3.2224","url":null,"abstract":"Death is an assured inevitability event for all living beings of this cosmos. Based on the American Academy of Neurology (AAN) recommendations, clinical diagnosis of Brain Death (BD) includes multiple sequences of tests. The Apnea Test (AT) is a more significant procedure in diagnosing BD. Unfortunately, complications during this procedure can occur more frequently than documented, and its incidence is limited to case reports. However, most critical care physicians and Cardio-respiratory therapists consider an AT a principal method to confirm BD as it provides decisive information about the definitive loss of brainstem function. Here, we describe the execution of the AT procedure that transpired into extensive subcutaneous emphysema, tension pneumothorax, and pneumoperitoneum followed by pulseless electrical activity due to direct airway perforation by the supplemental oxygen catheter. In addition, we do a literature review on airway complications incidence and suggest methods to avoid such fatal complications. Finally, further prospective studies are recommended to evaluate the barotrauma incidence and complication nature during AT.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45506055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Temporomandibular joint internal derangement (TMJ ID) may be managed by conservative means, minimally invasive methods, and/ or surgically. Conservative options include soft diet, pharmacotherapy, splints, physiotherapy, or topical administration of nutraceuticals, for elimination of TMJ ID symptoms. FIXIT is a topical ointment, consisting of plant-based ingredients; its specific components have been successful in treating knee osteoarthritis (OA). However, these have not been applied for TMJ ID conditions (lacuna in literature). The aim of this study is, therefore, to evaluate the effectiveness of FIXIT in TMJ ID management by assessing TMJ pain, clicking sound and pain-free maximum mouth opening (MMO) in an 18-year-old male. The patient complained of right TMJ pain (VAS- 8) on mouth opening, and loud clicking sounds, with pain-free MMO of 19 mm pre-treatment. Post-topical therapy (after 3 weeks) with FIXIT, significant reduction in TMJ pain (VAS- 0) and joint sounds (absent- 0), with improved MMO (47 mm) was noted. No recurrence of symptoms was observed after 3 months of follow-up. FIXIT has been effective in alleviating TMJ ID symptoms, thereby enhancing overall jaw function. Its ability to eliminate clicking sounds was found to be an unprecedented finding; suggesting it’ novelty as a non-surgical intervention for TMJ ID.
{"title":"A novel conservative modality in the management of internal derangement of temporomandibular joint- A case report","authors":"C. Ravikumar, Krishnakumar Raja V.B., Sasikala B.","doi":"10.51248/.v43i3.2747","DOIUrl":"https://doi.org/10.51248/.v43i3.2747","url":null,"abstract":"Temporomandibular joint internal derangement (TMJ ID) may be managed by conservative means, minimally invasive methods, and/ or surgically. Conservative options include soft diet, pharmacotherapy, splints, physiotherapy, or topical administration of nutraceuticals, for elimination of TMJ ID symptoms. FIXIT is a topical ointment, consisting of plant-based ingredients; its specific components have been successful in treating knee osteoarthritis (OA). However, these have not been applied for TMJ ID conditions (lacuna in literature). The aim of this study is, therefore, to evaluate the effectiveness of FIXIT in TMJ ID management by assessing TMJ pain, clicking sound and pain-free maximum mouth opening (MMO) in an 18-year-old male. The patient complained of right TMJ pain (VAS- 8) on mouth opening, and loud clicking sounds, with pain-free MMO of 19 mm pre-treatment. Post-topical therapy (after 3 weeks) with FIXIT, significant reduction in TMJ pain (VAS- 0) and joint sounds (absent- 0), with improved MMO (47 mm) was noted. No recurrence of symptoms was observed after 3 months of follow-up. FIXIT has been effective in alleviating TMJ ID symptoms, thereby enhancing overall jaw function. Its ability to eliminate clicking sounds was found to be an unprecedented finding; suggesting it’ novelty as a non-surgical intervention for TMJ ID.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42547750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: Diabetes mellitus (DM) will increasingly contribute to years of life and disability worldwide. Approximately 25% of individuals with diabetes will develop diabetic foot ulcers. This study aimed to assess the effectiveness of microcurrent therapy compared to low-level laser therapy in terms of wound healing and quality of life among patients with diabetic foot ulcers. Materials and Methods: This study followed a prospective randomized open blinded endpoint assessment (PROBE) design with two parallel groups allocated in a 1:1 ratio. Participants meeting the selection criteria and having diabetic foot ulcers were included. Baseline measurements were taken before randomly assigning participants to the treatment groups. Pre-test and post-test data were collected to evaluate the wound size. Results: The t-test analyses demonstrated significant improvement in both treatment groups (LLLT: t = 41.818, p < 0.001; Micro-Current Group: t = 32.787, p < 0.001). To compare the groups, One-Way Analysis of Variance (ANOVA) was conducted, indicating a significant difference in ulcer size between the LLLT group (mean value: 62.600, SD=3.035) and the microcurrent therapy group (mean value: 51.933, SD= 6.938). Conclusion: This study concludes that microcurrent therapy is more effective than low-level laser therapy for managing diabetic foot ulcers. The findings suggest that a 3-week intensive physiotherapy therapeutic approach, combined with conventional medical treatment, yields the best results in terms of wound healing.
{"title":"Efficacy of microcurrent therapy versus laser therapy for diabetic foot ulcer on size of the wound","authors":"K. M., C. S, Shruthi Kamal V.","doi":"10.51248/.v43i3.2696","DOIUrl":"https://doi.org/10.51248/.v43i3.2696","url":null,"abstract":"Introduction and Aim: Diabetes mellitus (DM) will increasingly contribute to years of life and disability worldwide. Approximately 25% of individuals with diabetes will develop diabetic foot ulcers. This study aimed to assess the effectiveness of microcurrent therapy compared to low-level laser therapy in terms of wound healing and quality of life among patients with diabetic foot ulcers.\u0000 \u0000Materials and Methods: This study followed a prospective randomized open blinded endpoint assessment (PROBE) design with two parallel groups allocated in a 1:1 ratio. Participants meeting the selection criteria and having diabetic foot ulcers were included. Baseline measurements were taken before randomly assigning participants to the treatment groups. Pre-test and post-test data were collected to evaluate the wound size.\u0000 \u0000Results: The t-test analyses demonstrated significant improvement in both treatment groups (LLLT: t = 41.818, p < 0.001; Micro-Current Group: t = 32.787, p < 0.001). To compare the groups, One-Way Analysis of Variance (ANOVA) was conducted, indicating a significant difference in ulcer size between the LLLT group (mean value: 62.600, SD=3.035) and the microcurrent therapy group (mean value: 51.933, SD= 6.938).\u0000 \u0000Conclusion: This study concludes that microcurrent therapy is more effective than low-level laser therapy for managing diabetic foot ulcers. The findings suggest that a 3-week intensive physiotherapy therapeutic approach, combined with conventional medical treatment, yields the best results in terms of wound healing.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49347505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Sahu, Ranjitha Rao, Chaithra G.V., Cheryl Sarah Philipose, Sneha Rao A. R., Vatsala K.B.
Amniotic band syndrome (ABS) is a rare condition in which there is entrapment of the growing foetus or its organs by the band of tissue formed from the amniotic membrane. We received 20 weeks’ gestation-old female foetus for pathological examination. On external gross examination foetus was detected with only the thumb in the right hand with the absence of the remaining four fingers. On thorough examination there was membranous structure attached to the right hand running towards the right leg and partly attached to the toes. This on microscopy showed fibrosis. ABS is a rare syndrome with varied presentations which sometimes can endanger the life of the foetus. Though exact aetiology is unknown till date various risk factors are attributed to the condition.
{"title":"Amniotic band syndrome- A rare case report","authors":"K. Sahu, Ranjitha Rao, Chaithra G.V., Cheryl Sarah Philipose, Sneha Rao A. R., Vatsala K.B.","doi":"10.51248/.v43i3.2495","DOIUrl":"https://doi.org/10.51248/.v43i3.2495","url":null,"abstract":"Amniotic band syndrome (ABS) is a rare condition in which there is entrapment of the growing foetus or its organs by the band of tissue formed from the amniotic membrane. We received 20 weeks’ gestation-old female foetus for pathological examination. On external gross examination foetus was detected with only the thumb in the right hand with the absence of the remaining four fingers. On thorough examination there was membranous structure attached to the right hand running towards the right leg and partly attached to the toes. This on microscopy showed fibrosis. ABS is a rare syndrome with varied presentations which sometimes can endanger the life of the foetus. Though exact aetiology is unknown till date various risk factors are attributed to the condition.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44544590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abhijnya K Renukaprasad, E. Suman, Udayalaxmi Jeppu, S. Paul
Introduction and Aim: Hospital-acquired infection with multidrug-resistant bacteria following urinary catheter use in hospitalized patients is mainly due to the biofilm-forming ability of these bacteria. It becomes essential to look at the other options of treatment of such urinary tract infections (UTI) and one such option has been the use of bacteriophages. The study aimed to compare the biofilms formed by uropathogenic Escherichia coli on three different types of urinary catheters in the presence of crude coliphage and sub-inhibitory concentration of ciprofloxacin. Materials and Methods: It was an Institution based experimental study. A total of 10 red rubber (latex) catheter pieces, 10 Foley (PVC) catheter pieces and 10 silicone Foley catheter pieces were used for the study. Crude coliphage suspension was prepared from sewage samples taken from the sewage treatment plant. Biofilm production by ten uropathogenic E. coli isolates on these catheters was determined by Kolter and O’Toole’s method with certain modifications. Biofilm assay was repeated with conditions using crude bacteriophage and sub-inhibitory concentration. An electron micrograph of the catheter pieces was taken to visualize the biofilm formed. P value of 0.05 was considered statistically significant. Results: It was observed that, in the presence of crude bacteriophage there was a significant reduction in the biofilm formation on all three catheters (p 0.05). Also, there was a significant reduction in biofilm production in the presence of sub-inhibitory concentration of ciprofloxacin on silicone Foley’s catheter (p=0.001) but not on red rubber catheter or PVC Foley’s catheter (p 0.05). It was also observed that in some of the isolates the colony counts increased following the addition of ciprofloxacin in case of PVC Foley catheters. Conclusion: Bacteriophage-coated catheters may be the gateway to overcoming the hurdle of multidrug-resistant catheter-associated urinary tract infection.
{"title":"Promising catheters to reduce hospital-acquired urinary tract infections","authors":"Abhijnya K Renukaprasad, E. Suman, Udayalaxmi Jeppu, S. Paul","doi":"10.51248/.v43i3.2603","DOIUrl":"https://doi.org/10.51248/.v43i3.2603","url":null,"abstract":"Introduction and Aim: Hospital-acquired infection with multidrug-resistant bacteria following urinary catheter use in hospitalized patients is mainly due to the biofilm-forming ability of these bacteria. It becomes essential to look at the other options of treatment of such urinary tract infections (UTI) and one such option has been the use of bacteriophages. The study aimed to compare the biofilms formed by uropathogenic Escherichia coli on three different types of urinary catheters in the presence of crude coliphage and sub-inhibitory concentration of ciprofloxacin.\u0000 \u0000Materials and Methods: It was an Institution based experimental study. A total of 10 red rubber (latex) catheter pieces, 10 Foley (PVC) catheter pieces and 10 silicone Foley catheter pieces were used for the study. Crude coliphage suspension was prepared from sewage samples taken from the sewage treatment plant. Biofilm production by ten uropathogenic E. coli isolates on these catheters was determined by Kolter and O’Toole’s method with certain modifications. Biofilm assay was repeated with conditions using crude bacteriophage and sub-inhibitory concentration. An electron micrograph of the catheter pieces was taken to visualize the biofilm formed. P value of 0.05 was considered statistically significant.\u0000 \u0000Results: It was observed that, in the presence of crude bacteriophage there was a significant reduction in the biofilm formation on all three catheters (p 0.05). Also, there was a significant reduction in biofilm production in the presence of sub-inhibitory concentration of ciprofloxacin on silicone Foley’s catheter (p=0.001) but not on red rubber catheter or PVC Foley’s catheter (p 0.05). It was also observed that in some of the isolates the colony counts increased following the addition of ciprofloxacin in case of PVC Foley catheters.\u0000 \u0000Conclusion: Bacteriophage-coated catheters may be the gateway to overcoming the hurdle of multidrug-resistant catheter-associated urinary tract infection.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41628557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. F., Vadlapudi Kumar, V. E., Poornima D.V., Anuradha C.M., Prathap H.M.
Introduction and Aim: Gnidia glauca (Fresen.) Gilg (G. glauca) is an abundant source of phenolic and flavonoid compounds with therapeutic potential, despite this very few investigations conducted for enhanced phenolic and flavonoid production by suspension culture. In the present study a non-destructive harvesting method was developed for phenolics and flavonoids production in cell suspension of G. glauca using methyl jasmonate (MeJA) as a potent elicitor for product enhancement. Materials and Methods: cell suspension culture was initiated to evaluate the elicitor impact on cell growth, Phytochemical extraction and screening, total phenolic and flavonoid content, antioxidant assay by 1,1 diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity, was done to evaluate, its antioxidant capacity in the methyl jasmonate elicited suspension cultured sample of G. glauca. Results: Methyl jasmonate elicitation in response to 50 µM dosage showed an increase in biomass production of phenolic compounds and flavonoids after 6 days of culture incubation. Qualitative screening of phytoconstituents revealed the abundance of flavonoids and phenolics tannins, confirmed by UV-Visible spectrophotometry, TLC and HPLC analyses. G. glauca cell suspension culture sample showed dose dependent DPPH free radical scavenging activity compared to the respective control samples. Conclusion: The outcome of the present study shows that G.glauca MeJA (methyl jasmonate) elicitation at 50µM dosage elicited the highest presence of phenolics and flavonoid with potential antioxidant capability. Considering the vulnerability of G.glauca as enlisted in endangered plants list, the present protocol is a non-destructive harvesting method to produce biologically active phenolics and flavonoids of the plant.
介绍与目的:灰刺荆(freen .)Gilg (G. glauca)是具有治疗潜力的酚类和类黄酮化合物的丰富来源，尽管通过悬浮培养提高酚类和类黄酮产量的研究很少。本研究利用茉莉酸甲酯(MeJA)作为强效激发子，建立了一种非破坏性的收获方法，用于青花毛藻细胞悬浮液中酚类物质和黄酮类物质的生产。材料与方法:通过细胞悬浮培养，评价激发子对细胞生长、植物化学提取与筛选、总酚类和类黄酮含量的影响，采用抗氧化法测定1,1二苯基-2-苦味酰肼(DPPH)自由基清除能力，在茉莉酸甲酯诱导的glauca悬浮培养样品中评价其抗氧化能力。结果:茉莉酸甲酯对50µM剂量的诱导，在培养6天后，酚类化合物和黄酮类物质的生物量增加。通过紫外-可见分光光度法、薄层色谱法和高效液相色谱法对其进行定性筛选，发现黄酮类化合物和酚类化合物单宁含量丰富。青光眼细胞悬浮培养样品与对照样品相比，具有剂量依赖性的DPPH自由基清除活性。结论:本研究结果表明，50µM剂量的茉莉酸甲酯(MeJA)诱导产生的酚类物质和类黄酮含量最高，具有潜在的抗氧化能力。考虑到青茅属濒危植物的脆弱性，本方案采用非破坏性采收方法，从青茅中提取具有生物活性的酚类物质和类黄酮。
{"title":"A non-destructive harvesting method to produce biologically active phenolics and flavonoids of Gnidia glauca (Fresen.) Gilg","authors":"R. F., Vadlapudi Kumar, V. E., Poornima D.V., Anuradha C.M., Prathap H.M.","doi":"10.51248/.v43i3.3191","DOIUrl":"https://doi.org/10.51248/.v43i3.3191","url":null,"abstract":"Introduction and Aim: Gnidia glauca (Fresen.) Gilg (G. glauca) is an abundant source of phenolic and flavonoid compounds with therapeutic potential, despite this very few investigations conducted for enhanced phenolic and flavonoid production by suspension culture. In the present study a non-destructive harvesting method was developed for phenolics and flavonoids production in cell suspension of G. glauca using methyl jasmonate (MeJA) as a potent elicitor for product enhancement. \u0000 \u0000Materials and Methods: cell suspension culture was initiated to evaluate the elicitor impact on cell growth, Phytochemical extraction and screening, total phenolic and flavonoid content, antioxidant assay by 1,1 diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging activity, was done to evaluate, its antioxidant capacity in the methyl jasmonate elicited suspension cultured sample of G. glauca. \u0000 \u0000Results: Methyl jasmonate elicitation in response to 50 µM dosage showed an increase in biomass production of phenolic compounds and flavonoids after 6 days of culture incubation. Qualitative screening of phytoconstituents revealed the abundance of flavonoids and phenolics tannins, confirmed by UV-Visible spectrophotometry, TLC and HPLC analyses. G. glauca cell suspension culture sample showed dose dependent DPPH free radical scavenging activity compared to the respective control samples. \u0000 \u0000Conclusion: The outcome of the present study shows that G.glauca MeJA (methyl jasmonate) elicitation at 50µM dosage elicited the highest presence of phenolics and flavonoid with potential antioxidant capability. Considering the vulnerability of G.glauca as enlisted in endangered plants list, the present protocol is a non-destructive harvesting method to produce biologically active phenolics and flavonoids of the plant.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48593328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: This study was aimed to investigate the impact of TENS (Transcutaneous Electrical Nerve Stimulation) along with Mckenzie lumbar extension exercise on patients with lumbar radiculopathy. The objective is to compare the effect of TENS with Mckenzie exercise and control group. The study assessed pain levels, radicular pain through SLR (Straight Leg Raise) test, disability using RMDQ (Roland Morris Disability Questionnaire), and spine range of motion (ROM) using MMST (Modified Modified Schober Test). Pre-test and post-test measurements were collected and compared to determine any differences. Methodology: The study included a total of 50 male and female patients aged between 31 and 40 years, all of whom experienced disabling pain for a duration of 6-12 weeks. The materials used for the assessment included an inch tape for measuring lumbar spine ROM, a goniometer for SLR, and a belt. Results: After the 6-week program, the findings revealed a significant reduction in pain levels, improved SLR, enhanced lumbar spine ROM, and decreased disability among the participants. Paired t-test showed significant differences in post-test values and independent t-test revealed significant post-test values in the experimental group than the control group at p<0.05. Conclusion: The group of patients with lumbar radiculopathy who received TENS combined with Mckenzie exercise demonstrated superior outcomes in terms of pain reduction, SLR improvement, MODI (Modified Oswestry Disability Index) scores, and RMQ (Roland Morris Questionnaire) scores compared to the group treated with general exercise and TENS.
{"title":"Effect of Mckenzie lumbar extension exercise with TENS on lumbar radiculopathy","authors":"S. Kumar B., Divyasree S., K. A, Surya Vishnuram","doi":"10.51248/.v43i3.2013","DOIUrl":"https://doi.org/10.51248/.v43i3.2013","url":null,"abstract":"Introduction and Aim: This study was aimed to investigate the impact of TENS (Transcutaneous Electrical Nerve Stimulation) along with Mckenzie lumbar extension exercise on patients with lumbar radiculopathy. The objective is to compare the effect of TENS with Mckenzie exercise and control group. The study assessed pain levels, radicular pain through SLR (Straight Leg Raise) test, disability using RMDQ (Roland Morris Disability Questionnaire), and spine range of motion (ROM) using MMST (Modified Modified Schober Test). Pre-test and post-test measurements were collected and compared to determine any differences.\u0000 \u0000Methodology: The study included a total of 50 male and female patients aged between 31 and 40 years, all of whom experienced disabling pain for a duration of 6-12 weeks. The materials used for the assessment included an inch tape for measuring lumbar spine ROM, a goniometer for SLR, and a belt.\u0000 \u0000Results: After the 6-week program, the findings revealed a significant reduction in pain levels, improved SLR, enhanced lumbar spine ROM, and decreased disability among the participants. Paired t-test showed significant differences in post-test values and independent t-test revealed significant post-test values in the experimental group than the control group at p<0.05.\u0000 \u0000Conclusion: The group of patients with lumbar radiculopathy who received TENS combined with Mckenzie exercise demonstrated superior outcomes in terms of pain reduction, SLR improvement, MODI (Modified Oswestry Disability Index) scores, and RMQ (Roland Morris Questionnaire) scores compared to the group treated with general exercise and TENS.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43960908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mamatha Shetty, Deepa Sara John, S. Durgapal, U. Shetty
Herpes simplex virus (HSV) is a double-stranded DNA virus that exists in two forms- HSV 1 and HSV 2. Oral herpetic lesions are typically caused by HSV-1. In the oral cavity, they are known to cause painful lesions that most commonly involve gingiva, palate, and buccal mucosa. Since it is a contagious infection and has the potential to cause complications in the host, timely diagnosis and management are important. Therefore, dental professionals must be aware of clinical presentation and appropriate diagnostic and management measures. The following case report discusses a case of Herpes Simplex Virus in a 33-year-old, systemically healthy female patient, who reported to the dental OPD with oral symptoms. The clinical features, investigations, diagnosis, and management of the condition have been elaborated in the review.
{"title":"Herpes simplex virus: A case report and review of literature","authors":"Mamatha Shetty, Deepa Sara John, S. Durgapal, U. Shetty","doi":"10.51248/.v43i3.2099","DOIUrl":"https://doi.org/10.51248/.v43i3.2099","url":null,"abstract":"Herpes simplex virus (HSV) is a double-stranded DNA virus that exists in two forms- HSV 1 and HSV 2. Oral herpetic lesions are typically caused by HSV-1. In the oral cavity, they are known to cause painful lesions that most commonly involve gingiva, palate, and buccal mucosa. Since it is a contagious infection and has the potential to cause complications in the host, timely diagnosis and management are important. Therefore, dental professionals must be aware of clinical presentation and appropriate diagnostic and management measures. The following case report discusses a case of Herpes Simplex Virus in a 33-year-old, systemically healthy female patient, who reported to the dental OPD with oral symptoms. The clinical features, investigations, diagnosis, and management of the condition have been elaborated in the review.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44864466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: Crude herbal drugs and commercial extracts obtained from plant sources continue to play a significant role in healthcare, particularly in developing countries where traditional medicine practices are deeply rooted. The aim of the study was to estimate the antioxidant (DPPH) and cytotoxic effects of the ethanolic extract of Flaveria trinervia on raw 246.7 cells. Materials and Methods: In the Indian town of Thoothukudi's Kovilpatti village, F. trinervia was isolated. The entire plant was air-dried before being crushed, and 1 kg of the powdered ethanolic extract was employed in the Soxhlet apparatus for continuous extraction. By utilizing GC-MS analysis, F. trinervia ethanolic extract phytochemical assessment was studied. The antioxidant potential of F. trinervia was examined using the DPPH test. Raw 246.7 cell lines were used to test the ethanolic extract of F. trinervia for cytotoxic activity. Results: F. trinervia extracts were found to contain a variety of biologically active phytoconstituents, including ergosterol, octadecanoic acid, propanoic acid, and isopropyl palmitate, according to phytochemical analysis. Compared to the negative control, the ethanolic extract of F. trinervia considerably showed antioxidant activity and cytotoxicity. Ascorbic acid antioxidant activity with DPPH was 98.12%, whereas F.trinervia ethanolic extract's antioxidant activity was 78.91%. The anticancer efficacy of F. trinervia ethanolic extract against uncultured 246.7 cells was evaluated using the MTT assay. By charting cell viability vs extract concentration, the IC50 value was determined. The MTT assay identified raw cells IC50 at 24 hrs as 200 µg/mL of ethanolic F. trinervia extract. Conclusion: Active phytoconstituents are present in the F. trinervia whole plant ethanolic extract. Because of the presence of phytoconstituents, the extracts have excellent antioxidant activity and good cytotoxicity activity against raw 246.7 cell lines. The study recommended using whole plant extract from F. trinervia to treat several metabolic diseases.
{"title":"Phytochemical analysis, in-vitro antioxidant and cytotoxicity activities of Flaveria trinervia ethanolic extract","authors":"Anju A. Varghese, A. Smith A., S. M.","doi":"10.51248/.v43i3.2526","DOIUrl":"https://doi.org/10.51248/.v43i3.2526","url":null,"abstract":"Introduction and Aim: Crude herbal drugs and commercial extracts obtained from plant sources continue to play a significant role in healthcare, particularly in developing countries where traditional medicine practices are deeply rooted. The aim of the study was to estimate the antioxidant (DPPH) and cytotoxic effects of the ethanolic extract of Flaveria trinervia on raw 246.7 cells.\u0000 \u0000Materials and Methods: In the Indian town of Thoothukudi's Kovilpatti village, F. trinervia was isolated. The entire plant was air-dried before being crushed, and 1 kg of the powdered ethanolic extract was employed in the Soxhlet apparatus for continuous extraction. By utilizing GC-MS analysis, F. trinervia ethanolic extract phytochemical assessment was studied. The antioxidant potential of F. trinervia was examined using the DPPH test. Raw 246.7 cell lines were used to test the ethanolic extract of F. trinervia for cytotoxic activity.\u0000 \u0000Results: F. trinervia extracts were found to contain a variety of biologically active phytoconstituents, including ergosterol, octadecanoic acid, propanoic acid, and isopropyl palmitate, according to phytochemical analysis. Compared to the negative control, the ethanolic extract of F. trinervia considerably showed antioxidant activity and cytotoxicity. Ascorbic acid antioxidant activity with DPPH was 98.12%, whereas F.trinervia ethanolic extract's antioxidant activity was 78.91%. The anticancer efficacy of F. trinervia ethanolic extract against uncultured 246.7 cells was evaluated using the MTT assay. By charting cell viability vs extract concentration, the IC50 value was determined. The MTT assay identified raw cells IC50 at 24 hrs as 200 µg/mL of ethanolic F. trinervia extract.\u0000 \u0000Conclusion: Active phytoconstituents are present in the F. trinervia whole plant ethanolic extract. Because of the presence of phytoconstituents, the extracts have excellent antioxidant activity and good cytotoxicity activity against raw 246.7 cell lines. The study recommended using whole plant extract from F. trinervia to treat several metabolic diseases.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45205430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction and Aim: In the global pandemic caused by Covid-19, older individuals and those with comorbidities have been shown to be more susceptible to serious diseases. The purpose of our research is to estimate the proportion of Covid-19 inpatients requiring blood transfusions over a period of 3 months. Materials and Methods: A retrospective observational analysis of blood product consumption in Covid-19 inpatients at our center from April to June 2021 was conducted by reviewing monthly transfusion records archived in our blood bank. Each blood component administered in Covid-19 wards during the prescribed period was noted. Results: Out of 1096 Covid-19 inpatients, only 66 (6.02%) patients received transfusions. As compared to non-Covid wards, Covid-19 wards had substantially lower transfusion rates for packed cell units (84 vs. 1074 units), FFP (33 vs. 691 units), and platelets (46 vs. 595 units). A total of 163 units of blood components were given to 66 Covid-19 inpatients, the majority of which were packed cell units (51.5%). Conclusion: In comparison to hospitalized non Covid patients, Covid-19 inpatients required fewer transfusions. In our institute, only the Covid 19 patients who were critically sick received blood transfusions; the rest received conservative therapy for their mild to moderate illnesses. This information will be beneficial for our department as it helps us plan and prepare for the use of blood supplies while the pandemic is still going strong.
{"title":"Changing trends in blood component utilisation during COVID time: Analysis of 1096 COVID-19 in patients","authors":"Christy K. Jose, K. Pailoor","doi":"10.51248/.v43i3.1334","DOIUrl":"https://doi.org/10.51248/.v43i3.1334","url":null,"abstract":"Introduction and Aim: In the global pandemic caused by Covid-19, older individuals and those with comorbidities have been shown to be more susceptible to serious diseases. The purpose of our research is to estimate the proportion of Covid-19 inpatients requiring blood transfusions over a period of 3 months.\u0000 \u0000Materials and Methods: A retrospective observational analysis of blood product consumption in Covid-19 inpatients at our center from April to June 2021 was conducted by reviewing monthly transfusion records archived in our blood bank. Each blood component administered in Covid-19 wards during the prescribed period was noted.\u0000 \u0000Results: Out of 1096 Covid-19 inpatients, only 66 (6.02%) patients received transfusions. As compared to non-Covid wards, Covid-19 wards had substantially lower transfusion rates for packed cell units (84 vs. 1074 units), FFP (33 vs. 691 units), and platelets (46 vs. 595 units). A total of 163 units of blood components were given to 66 Covid-19 inpatients, the majority of which were packed cell units (51.5%).\u0000 \u0000Conclusion: In comparison to hospitalized non Covid patients, Covid-19 inpatients required fewer transfusions. In our institute, only the Covid 19 patients who were critically sick received blood transfusions; the rest received conservative therapy for their mild to moderate illnesses. This information will be beneficial for our department as it helps us plan and prepare for the use of blood supplies while the pandemic is still going strong.","PeriodicalId":51650,"journal":{"name":"BioMedicine-Taiwan","volume":" ","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43293580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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