Current Research in Biotechnology最新文献

{"title":"Sustainable remediation of persistent organic Pollutants: A review on Recent innovative technologies","authors":"Fatihu Kabir Sadiq ,&nbsp;Abdulalim Ahovi Sadiq ,&nbsp;Tiroyaone Albertinah Matsika ,&nbsp;Barikisu Ahuoyiza Momoh","doi":"10.1016/j.crbiot.2025.100293","DOIUrl":"10.1016/j.crbiot.2025.100293","url":null,"abstract":"<div><div>Persistent organic pollutants (POPs) are harmful chemicals that remain in the environment for a long time due to their resistance to degradation. They represent a significant challenge to sustainable development due to their enduring presence and potential for bioaccumulation in the environment. This review examines emerging technologies for POP remediation, assessing conventional methods while emphasizing the potential of innovative approaches. Advanced technologies, including nanotechnology and advanced oxidation processes (AOPs), show potential for effective, cost-efficient and environmentally friendly POP degradation. Among AOPs, methods such as UV/H₂O₂, Fenton’s reaction, photo-Fenton, UV/Ozone, and photocatalysis with semiconductors like TiO₂ are particularly effective in breaking down POPs into less harmful substances. Nanotechnology-based methods provide high efficiency, selectivity, and reduced environmental impact, while photocatalysis offers a sustainable and eco-friendly approach to POP degradation. To enhance remediation effectiveness, integrating innovative materials, optimized processes, and artificial intelligence (AI) is crucial. Advancements in eco-friendly nanomaterials, hybrid AOPs, and AI-driven process optimization can significantly improve degradation efficiency, selectivity, and sustainability. Future research should focus on scaling up these technologies, improving cost-effectiveness, and incorporating AI-powered smart monitoring systems for long-term environmental management. A holistic and sustainable remediation strategy must also align with circular economy principles, promote eco-friendly innovations, and address the influence of climate change on POP behavior. By fostering interdisciplinary research, policy support, and international collaboration, the development of accessible and sustainable remediation solutions can be accelerated, contributing to a cleaner environment and a healthier future for all.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100293"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and population structure of Cnaphalocrocis medinalis across India and South Asia: Insights from COI and ITS2 gene analyses","authors":"Soumya Shephalika Dash ,&nbsp;Prasanthi Golive ,&nbsp;C. Parameswaran ,&nbsp;Prakash Chandra Rath ,&nbsp;Hirak Chatterjee ,&nbsp;Arup Kumar Mukherjee ,&nbsp;Partha Sarathi Tripathy ,&nbsp;Anjan Kumar Nayak ,&nbsp;Sucharita Mohapatra ,&nbsp;Bijay Kumar Behera ,&nbsp;Shyamaranjan Das Mohapatra","doi":"10.1016/j.crbiot.2025.100281","DOIUrl":"10.1016/j.crbiot.2025.100281","url":null,"abstract":"<div><div>Rice leaffolder, <em>Cnaphalocrocis medinalis</em>, a major pest of rice, poses a significant threat to crop yield across Asia. This is why the spread of this pest across Southeast Asia needs to be studied to understand its population dynamics, genetic diversity, and potential impact on regional rice cultivation. In this study, the genetic diversity and population structure of <em>C. medinalis</em> were examined using mitochondrial <em>COI</em> and nuclear <em>ITS2</em> gene sequences collected from 15 Indian states and several South Asian countries. A total of 100 <em>COI</em> and 63 <em>ITS2</em> sequences were analyzed, including new and publicly available data on NCBI, to assess nucleotide diversity, haplotype variation, and phylogenetic relationships. The DNA extraction, PCR amplification, sequencing, and bioinformatics analyses were done. Results revealed higher haplotype and nucleotide diversity in Indian populations compared to other Asian regions, indicating genetic variations and potential historical bottlenecks followed by population expansions. Median-joining haplotype networks showed widespread haplotypes, suggesting gene flow, while region-specific haplotypes indicated localized adaptation. AMOVA highlighted substantial genetic differentiation among populations for <em>COI</em> (62.51% variation), whereas <em>ITS2</em> showed moderate differentiation (43.86%). Phylogenetic analyses identified polyphyletic and monophyletic clades, emphasizing complex population dynamics. Neutrality tests and mismatch distributions suggested demographic expansions in Indian populations. These findings underscore the evolutionary history, possible migration pattern and genetic structure of <em>C. medinalis</em>, providing insights into pest management strategies. Future research should explore environmental and anthropogenic factors driving genetic differentiation to enhance integrated pest management practices, ensuring sustainable rice production across diverse agro-climatic regions.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100281"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143806872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review on Indian Acacia species","authors":"Bharat Singh ,&nbsp;Jagdish Prasad ,&nbsp;Ram Avtar Sharma","doi":"10.1016/j.crbiot.2025.100274","DOIUrl":"10.1016/j.crbiot.2025.100274","url":null,"abstract":"<div><div>Acacia plants are evergreen, perennial, short, flat-crowned to long and straight-bole trees. A total of 34 species of Acacia genus are distributed in different states of India. Acacia plants are used in the treatment of chronic dysentery, diarrhoea, throat troubles, conjunctivitis and stomatitis. The critical data of ethnopharmacological properties, phytochemical and pharmacological attributes of 27 species were obtained through extensive review of relevant reference books, M. Sc./M. Tech dissertations, PhD theses, Web of Science, PubMed, Google Scholar, ScienceDirect, Wiley, Taylors &amp; Francis, Bentham, Thieme, Springer, and eFloras. The flavone glycosides, flavonoids, lignans, anthraquinones, monoterpenes, and alkaloids have been identified from 20 species of Indian Acacia species. Indian Acacia species possess antimicrobial, antidiabetic, antiarthritic, antistress, anti-inflammatory, antioxidant, anticancer, and wound healing properties. However, various reports are available in literature on phytochemistry and biological properties of 27 Indian Acacia species but, still 7 species have not been evaluated for their pharmacological studies. The review discusses updated knowledge of ethnopharmacological applications, phytochemical and pharmacological attributes, and pharmacokinetic profiles of Indian Acacia species.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100274"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin dietary supplementation enhances serum carbohydrate-degrading enzyme activities in buffaloes","authors":"Maima Matin ,&nbsp;Natalia Ksepka ,&nbsp;Kamil Wysocki ,&nbsp;Michel-Edwar Mickael ,&nbsp;Alpana Pande ,&nbsp;Jarosław Olav Horbańczuk ,&nbsp;Artur Jóźwik ,&nbsp;Olga Adamska ,&nbsp;Artur Stolarczyk ,&nbsp;Laszlo Barna Iantovics ,&nbsp;Yordanka Ilieva ,&nbsp;Pencho Penchev ,&nbsp;Atanas G. Atanasov","doi":"10.1016/j.crbiot.2025.100276","DOIUrl":"10.1016/j.crbiot.2025.100276","url":null,"abstract":"<div><div>Curcumin, the major bioactive compound found in turmeric (<em>Curcuma longa</em>) is a subject of intensive research, due to its multiple bioactivities. Previous studies have investigated metabolic effects of curcumin in humans or established animal research models such as mice, but studies with farm animals have been scarce. In this work, we aimed to study the effects of curcumin supplementation in Bulgarian Murrah buffaloes (<em>Bubalus bubalis</em>), on the serum activities of seven important carbohydrate-metabolizing enzymes, β-glucuronidase, α-galactosidase, α-glucosidase, β-glucosidase, β-galactosidase, N-acetyl-hexosaminidase, and mannosidase. Curcumin was supplemented at a dose of 50 g per animal, per day, and serum activities of the studied enzymes were determined at the start (day 0), middle (day 14), and end (day 28) of the supplementation period. A tendency for increased activity of all studied enzymes was observed upon supplementation with curcumin, with the activity levels of five of the enzymes displaying statistically significant upregulation at day 28 (β-glucuronidase, α-galactosidase, β-galactosidase, N-acetyl-hexosaminidase, and mannosidase). These upregulated activities are in general indicative of increased carbohydrates turnover and detoxification processes, and might provide clues for a better understanding of the mechanisms of action of curcumin <em>in vivo</em>, as well as for novel approaches to rationally impact animal or human health and wellbeing through targeted supplementation.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100276"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143136749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implementation of Dunaliella tertiolecta and Desmodesmus communis in a photobioreactor prototype for treatment of wastewater in a recirculating aquaculture system","authors":"Kathleen P. Nolan,&nbsp;Jordan Roszell,&nbsp;Robert H. Hanner,&nbsp;Andreas Heyland","doi":"10.1016/j.crbiot.2025.100314","DOIUrl":"10.1016/j.crbiot.2025.100314","url":null,"abstract":"<div><div>Inline photobioreactors (PBRs) are a promising tool for nutrient removal from aquacultural wastewater and production of valuable algal biomass, yet few PBR systems have been rigorously tested. Optimization of algal growth screening across species and strains of interest under specific water conditions is crucial but time-consuming, limiting PBR implementation. Here, we developed a high-throughput screening system to efficiently test algal growth under various nutrient treatments, with the goal of informing implementation in a PBR designed for wastewater treatment in recirculating aquaculture systems (RAS). We assessed growth of the marine alga <em>Dunaliella tertiolecta</em> and the freshwater alga <em>Desmodesmus communis</em> under a matrix of inorganic nitrogen (N) treatments in 96-well plates. We then tested ammonium transfer within a prototype PBR for RAS wastewater treatment and evaluated the batch growth response of <em>D. tertiolecta</em> to ammonium treatments in the PBR. Both species grew on the provided inorganic N sources, showing significant differences in response to N treatments due to species-driven variations in nitrogen uptake and storage mechanisms. <em>D. tertiolecta</em> thrived when grown individually on either nitrate or ammonium, while <em>D. communis</em> favored a combination of N sources. <em>D. tertiolecta</em> showed a 5.4% higher growth rate in nitrate than ammonium. Both species grew in nutrient-free controls, suggesting potential use of internal nutrient reserves. <em>D. tertiolecta</em> grew within the PBR, serving as proof-of-concept for algal cultivation in the prototype. This study supports PBR technology for enhancing food production systems and protecting food security through RAS wastewater treatment.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100314"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative chemical biology tools for monitoring activity in complex microbiomes","authors":"Yumechris Amekan ,&nbsp;Kelly R. Redeker ,&nbsp;James P.J. Chong","doi":"10.1016/j.crbiot.2025.100334","DOIUrl":"10.1016/j.crbiot.2025.100334","url":null,"abstract":"<div><div>The functional analysis of complex microbiomes is hindered by their cellular heterogeneity and dynamic interactions. Conventional approaches often lack the resolution to resolve the metabolic activity of individual cells in situ. Recent advances in chemical biology have introduced powerful tools—such as bioorthogonal chemistry, stable isotope probing (SIP), and single-cell phenotyping—that enable non-destructive, high-resolution profiling of microbial activity across diverse ecosystems. These techniques bridge the gap between genotype and phenotype by targeting translational and metabolic functions in live cells, including uncultured or low-abundance taxa. This review outlines the principles, applications, and current limitations of these tools, including challenges in probe biocompatibility, throughput, and spectral or isotopic data analysis. We highlight recent innovations, including BONCAT-FACS integration, automated SIP platforms, and microfluidic Raman-activated cell sorting (RACS), which enhance analytical scalability. Emphasis is placed on the integration of chemical biology tools with multi-omics workflows to generate causal insights into microbial function. By addressing key technical and analytical barriers, these tools promise to expand our capacity to monitor and manipulate microbiomes for applications in ecology, biotechnology, and health. Their continued development will be critical for unlocking the functional potential of microbial communities across environmental and engineered systems.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100334"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145047871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurotensin(8-13) analogs targeting NTS1 and NTS2 receptors: A comparative in vitro and molecular modeling study","authors":"Kiril Kirilov ,&nbsp;Maria Ponticelli ,&nbsp;Toni Kühl ,&nbsp;Harald Hübner ,&nbsp;Maya G. Georgieva ,&nbsp;Matthias Vogel ,&nbsp;Aneliya A. Balacheva ,&nbsp;Bodo Haas ,&nbsp;Tamara I. Pajpanova ,&nbsp;Maima Matin ,&nbsp;Luigi Milella ,&nbsp;Peter Gmeiner ,&nbsp;Diana Imhof ,&nbsp;Nikolay T. Tzvetkov","doi":"10.1016/j.crbiot.2025.100298","DOIUrl":"10.1016/j.crbiot.2025.100298","url":null,"abstract":"<div><div>The simultaneous activation of both neurotensin type 1 and 2 receptors (NTS1R and NTS2R) through the neuronal peptide neurotensin (NT), activating the dopamine (DA) release and DA signaling within the dopaminergic system in the brain, suggest that NTS1R/NTS2R dual-specific NT analogs may represent an attractive tool in the treatment of Parkinson’s disease (PD) and/or other related conditions. Herein, we report <em>in silico</em> exploration of NTS1R and NTS2R driven by <em>in vitro</em> pharmacological evaluation of the linear hexapeptide NT analogs <strong>3</strong> (sequence Lys⁸-Cav⁹-Pro¹⁰-Tyr¹¹-Ile¹²-Leu¹³) and <strong>6</strong> (Arg⁸-Cav⁹-Pro¹⁰-Tyr¹¹-Ile¹²-Leu¹³), both active towards the human NTS1R and NTS2R. Compared to the parent peptide NT(8–13) (<strong>2</strong>), compounds <strong>3</strong> and <strong>6</strong> showed improved <em>in vitro</em> human plasma stability and BBB permeability. Moreover, <em>in silico</em> ADMET evaluation indicated that both NT-analogs have strong pharmacological properties combined with good safety profiles, highlighting their potential for further structural improvements. Furthermore, we applied an AI-based approach to generate the homology models of hNTS1R and hNTS2R, followed by MD simulations of their ligand-free state and molecular docking in order to estimate the most probable protein–ligand complexes of peptides <strong>3</strong> and <strong>6</strong>. Binding interaction/affinity analysis of the best-ranked docking modes, obtained with selected time-frames from the respective MD trajectories, suggest that the receptor activation occurs via a ligand-receptor binding into the initial “entry” conformation of hNTS1R and hNTS2R. This assumption is supported by additional HYDE analysis confirming the binding affinities of peptides <strong>3</strong> and <strong>6</strong> towards hNTS1R and hNTS2R obtained by radioligand binding experiments. The reported study may serve as a ready-to-use <em>in silico</em> approach for further development of therapeutic options against PD and potentially other neurological disorders.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100298"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modern and traditional strategies for controlling microbial contamination in plant micropropagation: Current insights and future perspectives","authors":"Nandang Permadi ,&nbsp;Dedat Prismantoro ,&nbsp;Pramesthi Reitza Navisya Vasall ,&nbsp;Farah Aprisza Sheelmarevaa ,&nbsp;Sarifah Nurjanah ,&nbsp;Mohamad Nurzaman ,&nbsp;Arshad Naji Alhasnawi ,&nbsp;Febri Doni ,&nbsp;Euis Julaeha","doi":"10.1016/j.crbiot.2025.100337","DOIUrl":"10.1016/j.crbiot.2025.100337","url":null,"abstract":"<div><div>Plant tissue culture is an essential technique widely utilized in agriculture, horticulture, scientific research, and biodiversity preservation, offering controlled methods for plant multiplication and genetic modification. This approach contributes to increased agricultural productivity, enhanced disease resistance, and the protection of rare or threatened plant species. However, microbial contamination—originating from microorganisms in the phyllosphere, rhizosphere, and endosphere—remains a major challenge in plant micropropagation. Without effective management, contamination can inhibit regeneration, callus formation, adventitious shoot development, and may even lead to tissue death. Various strategies, including thermotherapy, chemotherapy, and cryotherapy, have been employed to combat contamination in plant micropropagation. Moreover, emerging approaches such as the application of nanotechnology and natural products offer promising, eco-friendly alternatives. This review examines the sources of contamination and highlights the strategies used to mitigate it, offering insights into innovative solutions that support sustainable agricultural practices and conservation efforts.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100337"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating probiotics and microbiome-derived metabolites into cancer therapy: mechanistic insights, multi-omics strategies, and clinical potential","authors":"Zahrasadat Seyyedi ,&nbsp;Hamed Haddad Kashani ,&nbsp;Atefeh Parchebafi ,&nbsp;Romina Ghayoumi ,&nbsp;Mohammad Mehdi Haghighat Lari ,&nbsp;Elahe Seyed Hosseini","doi":"10.1016/j.crbiot.2025.100340","DOIUrl":"10.1016/j.crbiot.2025.100340","url":null,"abstract":"<div><div>Probiotics and their microbiome-derived metabolites are increasingly recognized as key players in cancer biology, functioning not only as regulators of gut homeostasis but also as modulators of tumor development, therapeutic response, and treatment-associated toxicities. These beneficial microorganisms exert their effects through diverse mechanisms, including microbiota remodeling, pathogen suppression, immune reprogramming, epigenetic regulation, and production of bioactive metabolites such as short-chain fatty acids, bacteriocins, vitamins, and exopolysaccharides. These metabolites influence fundamental processes of tumorigenesis by inducing apoptosis, inhibiting proliferation, modulating angiogenesis, and reshaping the tumor microenvironment toward a more immunoresponsive phenotype. Importantly, probiotics have been shown to enhance the efficacy of immune checkpoint inhibitors, mitigate chemotherapy- and radiotherapy-induced gastrointestinal complications, and detoxify carcinogens, thereby acting as supportive adjuncts in oncology. Emerging computational biology and multi-omics approaches—including genomics, metabolomics, and transcriptomics—have accelerated the identification of biosynthetic gene clusters and metabolic pathways responsible for anticancer bioactivity, allowing mechanistic mapping of probiotic–host–tumor interactions. Integration of these datasets highlights the potential for precision-guided probiotic formulations and engineered microbial consortia as novel therapeutic platforms. Nevertheless, clinical translation is hindered by strain-specific variability, host microbiome heterogeneity, lack of standardized protocols, and limited large-scale randomized trials. Addressing these gaps through harmonized methodologies, deeper mechanistic validation, and patient-tailored microbiome profiling will be essential for transforming probiotics from supportive dietary supplements into precision-based oncology therapeutics. This review integrates mechanistic insights, multi-omics strategies, and clinical evidence on probiotics and their metabolites in cancer therapy. By converging advances in microbial biotechnology, systems biology, and translational oncology, we propose probiotics as sustainable and precision-driven agents capable of reshaping the landscape of cancer prevention and treatment.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100340"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Life cycle and environmental impact assessment of vegetation-activated sludge process (V-ASP) for decentralized wastewater treatment","authors":"Pu Li ,&nbsp;Xiaomeng Hu ,&nbsp;Jiajia Yuan ,&nbsp;Feiyun Sun ,&nbsp;Pengfei Li ,&nbsp;Wenyi Dong ,&nbsp;Erdeng Du ,&nbsp;Mingguo Peng","doi":"10.1016/j.crbiot.2023.100172","DOIUrl":"10.1016/j.crbiot.2023.100172","url":null,"abstract":"<div><p>The integrated Vegetation-Activated Sludge Process (V-ASP) has emerged as a promising solution for decentralized wastewater treatment, offering a unique combination of landscape vegetation with the traditional Anaerobic-Anoxic-Oxic process (AAO). The experiments displayed that V-ASP consistently demonstrated remarkable treatment performance, maintaining stable removal efficiency exceeding 90 % for COD, NH₄⁺-N, and TP. The vegetation growth is helpful for the removal of pollutants and the microbial community in the allocated vegetation root, while the bulk suspended sludge was changed significantly by using PCR test. To comprehensively evaluate the environmental footprint of V-ASP, a Life Cycle Assessment (LCA) was conducted. The mass balance calculation containing wastewater treatment performance, energy consumption, vegetation growth behavior, greenhouse gas (GHG) emissions, treated water, and sludge discharge was carried out to establish the life cycle inventories (LCI). SimaPro 9.0 software and the ReCiPe (H) midpoint impact assessment method were employed, which revealed that the V-ASP system boasts low GHG emissions and freshwater eutrophication potential compared to the traditional AAO process. In essence, this study provides a comprehensive understanding of the V-ASP system, especially the environmental impacts, guiding its potential for sustainable decentralized sewage treatment applications.</p></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"7 ","pages":"Article 100172"},"PeriodicalIF":5.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590262823000540/pdfft?md5=b199791f2cdc6d6f47b7448ee381f95e&pid=1-s2.0-S2590262823000540-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139023335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}