Current Research in Biotechnology最新文献_第9页

Gut microbiota modulation and health benefits of various fasting regimens 肠道菌群调节和各种禁食方案的健康益处

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 Epub Date: 2025-07-03 DOI: 10.1016/j.crbiot.2025.100311

Sumel Ashique , Biplab Debnath , Naheed Mojgani , Mohd. Tariq , Tanweer Haider , Eman Shorog , Sabina Yasmin , Anas Islam , Himanshu Sharma , Md Sadique Hussain , Ayush Madan , Tabarak Malik , Mohammad Yousuf Ansari

Fasting is a diet intervention that has been shown to significantly modulate the gut microbiota, with potential health benefits. This review examines the impact of various fasting regimens, including time-restricted eating (TRE), Ramadan fasting (RF), and long-term fasting, on the composition and function of the gut microbiota in both animal models and human subjects. Studies have shown that fasting can increase microbial diversity, enrich beneficial bacteria such as Faecalibacterium prausnitzii, Roseburia spp., Akkermansia muciniphila, and other Lachnospiraceae, and enhance the production of short-chain fatty acids (SCFA) such as butyrate. These microbiota changes are associated with improvements in metabolic health markers, including body weight, blood pressure, glucose regulation, and lipid profiles, as well as reduced inflammation. However, the persistence of these effects after fasting varies between studies. Dietary changes during fasting, such as meal timing and composition, play a crucial role in shaping the response of the gut microbiota.Although most studies report beneficial results, heterogeneity in results highlights the need for more research to elucidate the relative contributions of factors such as age, baseline diet, habitual microbiota composition, fasting duration, and caloric content. The mechanistic links between specific microbial metabolites and clinical endpoints remain limited, and evidence for the synergistic effects of probiotics with fasting in humans is inconclusive. Future studies should employ standardized fasting protocols, integrate multi-omics approaches, and extend follow-up periods to determine the durability of microbiota and metabolic shifts. Developing personalized fasting strategies tailored to an individual’s baseline microbiota and metabolic phenotype may optimize the therapeutic potential of fasting for gut and metabolic health.

禁食是一种饮食干预，已被证明可以显著调节肠道微生物群，具有潜在的健康益处。本综述研究了各种禁食方案，包括限时饮食（TRE）、斋月禁食（RF）和长期禁食，对动物模型和人类受试者肠道微生物群的组成和功能的影响。研究表明，禁食可以增加微生物多样性，丰富有益菌，如Faecalibacterium prausnitzii， Roseburia spp.， Akkermansia muciniphila等毛螺科菌，并促进丁酸盐等短链脂肪酸（SCFA）的产生。这些微生物群的变化与代谢健康指标的改善有关，包括体重、血压、血糖调节和脂质谱，以及炎症的减少。然而，在不同的研究中，禁食后这些影响的持续程度有所不同。禁食期间的饮食变化，如用餐时间和组成，在塑造肠道微生物群的反应中起着至关重要的作用。尽管大多数研究报告了有益的结果，但结果的异质性强调了需要更多的研究来阐明年龄、基线饮食、习惯微生物群组成、禁食时间和热量含量等因素的相对贡献。特定微生物代谢物与临床终点之间的机制联系仍然有限，益生菌与人类禁食的协同作用的证据尚无定论。未来的研究应采用标准化的禁食方案，整合多组学方法，并延长随访时间，以确定微生物群的持久性和代谢变化。根据个体的基线微生物群和代谢表型制定个性化的禁食策略，可能会优化禁食对肠道和代谢健康的治疗潜力。

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引用次数: 0

Functional characterization of a secreted glucansucrase from Leuconostoc mesenteroides and the potential of its α-glucan product in oxygen stress relief 肠系膜白斑菌分泌的葡聚糖酶的功能特征及其α-葡聚糖产物在氧应激缓解中的潜力

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 Epub Date: 2025-11-09 DOI: 10.1016/j.crbiot.2025.100350

Huafeng Feng , Bing-hua Wang , Chunping You , Jin Han , Kexin Jiang , Yanni Zhou , Hei-tsai Tabγač , Peng Chang , Wei Shen , Minghui Yan

Leuconostoc mesenteroides are characterized by α-glucan production in the presence of sucrose. Although they lack the enzymes to scavenge reactive oxygen species (ROS), strains of L. mesenteroides are generally tolerant to oxygen. Despite the wide applications of L. mesenteroides and α-glucans in food and medical industry, mechanisms underlie α-glucan synthesis, and their roles in oxygen-tolerance remain to be elucidated. This study aimed at functional characterization of the enzyme for α-glucan synthesis in L. mesenteroides CGMCC10064 and addressing its role in oxygen stress. 1323 was identified as a secreted glucansucrase via SDS-PAGE followed by MALDI-TOF MS and Western blot. Its enzyme activity was characterized via in situ polymer synthesis and function blockade by a specific antibody. Finally, 1323-encoding gene was cloned and expressed in E. coli. α-glucan was synthesized in vitro with recombinant 1323 and its potential in oxygen stress relief was investigated. The α-glucan obtained improved the aerobic growth of L. mesenteroides strains via reduction of the oxidation–reduction potential as well as ROS accumulation. Moreover, oxygen stress relief by the α-glucan improved the growth and survival of probiotic Lactobacillus and Bifidobacterium strains under oxygen stress. These results highlighted the potential of a novel secreted glucansucrase and its α-glucan product in oxygen stress relief and cultivation and preservation of oxygen-sensitive bacteria.

肠系膜白色菌的特点是在有蔗糖存在的情况下产生α-葡聚糖。虽然肠系膜乳杆菌缺乏清除活性氧（ROS）的酶，但它们通常对氧具有耐受性。尽管肠系膜乳杆菌和α-葡聚糖在食品和医疗工业中有着广泛的应用，但α-葡聚糖的合成机制及其在耐氧中的作用仍有待阐明。本研究旨在对肠系膜乳酸菌（L. mesenteroides）中α-葡聚糖合成酶CGMCC10064的功能特征及其在氧胁迫中的作用进行研究。通过SDS-PAGE、MALDI-TOF MS和Western blot鉴定1323为分泌型葡聚糖酶。通过原位聚合物合成和特异性抗体的功能阻断来表征其酶活性。最后克隆了1323编码基因并在大肠杆菌中表达。用重组1323体外合成α-葡聚糖，并探讨其在氧应激中的应用潜力。得到的α-葡聚糖通过降低氧化还原电位和ROS积累，促进了肠系膜乳杆菌的好氧生长。α-葡聚糖对氧胁迫下益生菌乳酸菌和双歧杆菌的生长和存活均有促进作用。这些结果突出了一种新的分泌型葡聚糖酶及其α-葡聚糖产物在氧应激缓解和氧敏感细菌的培养和保存方面的潜力。

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引用次数: 0

Mosquitoes bite cancer patients, but never get cancer: what can we learn? digestive enzymes and host-specificity as natural barriers 蚊子叮咬癌症患者，但永远不会得癌症：我们能学到什么？消化酶和宿主特异性作为天然屏障

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 Epub Date: 2025-11-19 DOI: 10.1016/j.crbiot.2025.100353

Riza-Arief Putranto , Fiona Nishani , Erda Qorri , Raymond Rubianto Tjandrawinata , Antonello Santini , Fahrul Nurkolis

Mosquitoes, despite their frequent exposure to various mammalian blood components, including potentially aberrant cells, have never been reported to develop cancer-like conditions. This paper proposes a speculative biological model suggesting that the mosquito’s digestive and immune systems could naturally prevent the survival of malignant cells, offering inspiration for bioinspired cancer-therapeutic design. This perspective explores how the mosquito midgut creates an inhospitable environment through rapid pH shifts, potent digestive enzymes, and oxidative stress, leading to potential degradation of mammalian cancer cells. In parallel, the mosquito’s innate immune system, including antimicrobial peptides (AMPs), hemocyte activity, melanization, and reactive oxygen species (ROS), further neutralizes non-self-cellular threats with speed and precision. Together, these digestive and immune mechanisms offer valuable inspiration for cancer therapy design. This perspective posits that the mosquito’s intrinsic mechanisms for xenobiotic clearance and immune defense against diverse biological threats offer a unique, yet underexplored, biomimetic blueprint for overcoming key challenges in contemporary oncology, particularly drug resistance and the need for precision targeting. Emerging strategies such as ROS-based treatments, protease-mimetic systems, and AMP-engineered platforms reflect the potential of translating mosquito-derived mechanisms and their barriers into biomedical applications and drug delivery systems strategies. This article underscores how the mosquito’s natural defenses may guide the development of novel, bioinspired approaches to improve cancer treatment. Even though this idea remains speculative with limited direct experimental proof, investigating these mechanisms may offer inspiration for the design of bioinspired cancer therapies in the future.

尽管蚊子经常接触各种哺乳动物血液成分，包括潜在的异常细胞，但从未有报道称它们会患上类似癌症的疾病。本文提出了一个推测性的生物学模型，表明蚊子的消化和免疫系统可以自然地阻止恶性细胞的生存，为生物启发的癌症治疗设计提供了灵感。这一观点探讨了蚊子中肠如何通过快速的pH值变化、有效的消化酶和氧化应激创造一个不适宜生存的环境，从而导致哺乳动物癌细胞的潜在降解。与此同时，蚊子的先天免疫系统，包括抗菌肽（AMPs）、血细胞活性、黑色素化和活性氧（ROS），进一步以快速和精确的方式中和非自身细胞的威胁。总之，这些消化和免疫机制为癌症治疗设计提供了宝贵的灵感。这一观点认为，蚊子清除外源和免疫防御各种生物威胁的内在机制为克服当代肿瘤学的关键挑战，特别是耐药性和精确靶向的需要，提供了一个独特的，但尚未得到充分探索的仿生蓝图。诸如基于ros的治疗、蛋白酶模拟系统和amp工程平台等新兴策略反映了将蚊子衍生机制及其障碍转化为生物医学应用和药物输送系统策略的潜力。这篇文章强调了蚊子的自然防御如何指导新的、受生物启发的方法来改善癌症治疗的发展。尽管这种想法仍然是推测性的，直接实验证据有限，但研究这些机制可能会为未来设计生物启发癌症疗法提供灵感。

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引用次数: 0

Eugenol’s anti-cancer properties, its modulation of signalling pathways, and cascades across various cancers: A review 丁香酚的抗癌特性，其信号通路的调节，和级联在各种癌症：综述

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 Epub Date: 2025-08-28 DOI: 10.1016/j.crbiot.2025.100330

Anirban Debnath , Yi-Hao Lo , Manojit Bhattacharya , Zhi-Hong Wen , Chiranjib Chakraborty , Arpita Das

Several cancer drugs show drug resistance, which is a significant concern for cancer therapy. Therefore, new molecules are required for cancer treatment as anticancer drugs. Recently, several natural bioactive components from medicinal plants have demonstrated anticancer activity and are being marketed as anticancer drugs. Eugenol, a significant bioactive compound, is found in cloves and other traditional Indian medicinal plants, such as cinnamon, tulsi, ginger, turmeric, and Japanese star anise, which have been reported to have significant anticancer properties. The present review highlights the anticancer properties of eugenol. It also exhibits different pharmacological effects (anti-inflammatory, cardio-protection, and neuroprotection). As an anticancer molecule, eugenol exhibits anti-apoptotic, anti-angiogenic, and anti-metastatic properties in cancer cell lines and in vivo animal models, which we discuss in this review. We also discuss eugenol’s effect on different signaling pathways in cancer. Finally, recent diverse anticancer studies of eugenol have been discussing the different cancer types, such as breast cancer, gastric cancer, liver cancer, lung cancer, prostate cancer, ovarian cancer, cervical cancer, gliomas, oral cancers, skin cancer, leukemia, colorectal cancers, osteosarcoma, melanomas, etc. The current review provides an insightful overview of the anticancer properties of eugenol in the treatment and prevention of cancer, ultimately benefiting society.

一些抗癌药物显示出耐药性，这是癌症治疗的一个重要问题。因此，癌症治疗需要新的分子作为抗癌药物。近年来，从药用植物中提取的几种天然生物活性成分已显示出抗癌活性，并作为抗癌药物推向市场。丁香酚是一种重要的生物活性化合物，存在于丁香和其他传统的印度药用植物中，如肉桂、土尔西、生姜、姜黄和日本八角茴香，据报道，这些植物具有显著的抗癌特性。本文综述了丁香酚的抗癌作用。它还表现出不同的药理作用（抗炎、心脏保护和神经保护）。丁香酚作为一种抗癌分子，在癌细胞系和体内动物模型中表现出抗凋亡、抗血管生成和抗转移的特性，本文就其进行综述。我们还讨论了丁香酚对癌症中不同信号通路的影响。最后，近年来丁香酚的各种抗癌研究都在讨论不同的癌症类型，如乳腺癌、胃癌、肝癌、肺癌、前列腺癌、卵巢癌、宫颈癌、胶质瘤、口腔癌、皮肤癌、白血病、结直肠癌、骨肉瘤、黑色素瘤等。本文综述了丁香酚在治疗和预防癌症方面的抗癌特性，最终造福社会。

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引用次数: 0

Metagenomics of the microbial consortium in the anaerobic co-digestion of food waste and poultry droppings 食物垃圾和家禽粪便厌氧共消化微生物群的宏基因组学研究

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 Epub Date: 2025-08-25 DOI: 10.1016/j.crbiot.2025.100328

Oluwatomisin A. Akinsola , Samuel O. Dahunsi , Ebenezer L. Odekanle , Olajire D. Adedokun

The anaerobic co-digestion of food waste and poultry droppings presents a valuable strategy for achieving sustainable waste management and generating renewable energy. In this study, metagenomic analysis was utilized to investigate the microbial consortium driving the process, with a particular focus on the functional potential and interactions among microbial populations. DNA was extracted from the samples collected from the digester. The16S rRNA sequencing was used for the taxonomic diversity and functional profiling of the microbial community derived from the anaerobic digester. The findings reveal a complex interplay of diverse microbial taxa, predominantly Firmicutes, and Bacteroidetes, which are crucial for the degradation of organic matter and biogas production. The metabolic pathways utilized by the consortium were highlighted. The application of metagenomic tools, such as QIIME2 for data cleaning, BV-BRC for functional annotation, and PICRUSt for metabolic pathway analysis, facilitated a comprehensive functional profiling of the community, allowing the assessment of the contributions of individual taxa to the overall metabolic processes. The results contribute to the growing body of knowledge on microbial ecology in waste treatment systems and pave the way for future research aimed at optimizing anaerobic digestion processes for sustainable energy production.

食物垃圾和家禽粪便的厌氧共消化是实现可持续废物管理和产生可再生能源的宝贵战略。在这项研究中，利用宏基因组分析来研究驱动这一过程的微生物联盟，特别关注微生物群体之间的功能潜力和相互作用。从消化器收集的样本中提取DNA。16s rRNA测序用于厌氧消化池微生物群落的分类多样性和功能分析。这些发现揭示了不同微生物类群的复杂相互作用，主要是厚壁菌门和拟杆菌门，它们对有机物的降解和沼气的产生至关重要。强调了该联盟利用的代谢途径。应用宏基因组工具，如QIIME2进行数据清理，BV-BRC进行功能注释，PICRUSt进行代谢途径分析，促进了群落的全面功能分析，从而可以评估单个分类群对整体代谢过程的贡献。这些结果有助于废物处理系统中微生物生态学知识的增长，并为未来旨在优化厌氧消化过程以实现可持续能源生产的研究铺平道路。

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引用次数: 0

Eco-engineered remediation: Microbial and rhizosphere-based strategies for heavy metal detoxification 生态工程修复：基于微生物和根际的重金属解毒策略

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 Epub Date: 2025-05-08 DOI: 10.1016/j.crbiot.2025.100297

Arun Karnwal , Gaurav Kumar , Alaa El Din Mahmoud , Joydeep Dutta , Rattandeep Singh , Abdel Rahman Mohammad Said Al-Tawaha , Tabarak Malik

Heavy metal (HM) contamination significantly threatens ecosystems and human health. This review explores eco-engineered bioremediation strategies, focusing on the pivotal role of rhizosphere-associated microorganisms in detoxifying heavy metals. Rhizobacteria deploy diverse mechanisms—including biosorption, bioaccumulation, biotransformation, and biomineralization—to immobilize or convert toxic metals, with their efficiency strongly influenced by environmental factors such as pH and metal speciation. Plant Growth-Promoting Rhizobacteria (PGPR) further enhance phytoremediation by mitigating metal-induced phytotoxicity and promoting plant resilience under stress. Various scalable approaches, including in-situ and ex-situ remediation techniques, biosorbents, microbial consortia, and genetically engineered microbes (GEMs), show promising potential but raise essential ecological and regulatory concerns. Key challenges such as scalability, environmental variability, and the possible formation of toxic intermediates must be carefully addressed. Advances in omics technologies and a deeper exploration of native microbial communities offer promising avenues to optimize bioremediation outcomes. Moreover, a detailed understanding of plant–microbe interactions and the role of secondary metabolite signalling in the rhizosphere is essential to improve remediation efficiency. Future strategies should prioritize the application of functional genomics, developing bioinoculants tailored to specific environmental conditions, and implementing robust ecological risk assessments for GEMs. This review underscores the need for a multidisciplinary approach- integrating microbial ecology, plant sciences, and environmental engineering- to drive the development of sustainable, effective HM remediation technologies worldwide.

重金属污染严重威胁着生态系统和人类健康。本文综述了生态工程生物修复策略，重点介绍了根际相关微生物在重金属解毒中的关键作用。根细菌利用多种机制——包括生物吸附、生物积累、生物转化和生物矿化——来固定或转化有毒金属，其效率受到pH和金属形态等环境因素的强烈影响。植物促生长根瘤菌（Plant growth promoting Rhizobacteria， PGPR）通过减轻金属诱导的植物毒性和促进植物在逆境下的恢复能力，进一步增强植物的修复能力。各种可扩展的方法，包括原位和非原位修复技术、生物吸附剂、微生物联合体和基因工程微生物（GEMs），显示出有希望的潜力，但引起了基本的生态和监管问题。关键的挑战，如可扩展性、环境可变性和有毒中间体的可能形成，必须仔细解决。组学技术的进步和对原生微生物群落的深入探索为优化生物修复结果提供了有希望的途径。此外，详细了解植物与微生物的相互作用以及根际次生代谢物信号的作用对于提高修复效率至关重要。未来的战略应优先考虑功能基因组学的应用，开发适合特定环境条件的生物接种剂，并对GEMs实施强有力的生态风险评估。这篇综述强调了需要一个多学科的方法-整合微生物生态学，植物科学和环境工程-推动可持续的，有效的HM修复技术在世界范围内的发展。

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引用次数: 0

Peptides and peptidomimetics in the development of hydrogels towards the treatment of diabetic wounds 水凝胶在糖尿病伤口治疗中的应用

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 Epub Date: 2025-04-23 DOI: 10.1016/j.crbiot.2025.100292

Ana Gomes, Paula Gomes

Diabetic foot ulcers are a major complication of diabetes and a particularly complex type of chronic wounds, as most diabetes-related physiological dysregulations impair healing and increase the risk of infection. Despite many health literacy initiatives have been deployed to promote both the prevention and the proper management of diabetic foot ulcers, these remain a serious healthcare problem whose incidence is rising. This demands not only for a continuous updating of guidelines for adequate interventions in healthcare facilities, but also for new topical treatments able to improve the health and well-being of the patients. In this later case, current options include topical oxygen therapy, negative pressure wound therapy, wound dressings of different types, and topical gels. Due to the tremendous potential of hydrogel-based dressings for diabetic wound treatment, several hydrogels from either natural or synthetic origin have been explored. The hydrogel polymeric matrices can be loaded with many diverse molecules (cargoes) eventually combining distinct therapeutic actions. In this context, peptides are gaining relevance, which is not limited to those used as bioactive cargoes, since self-assembling peptides able to form hydrogels are also under the spotlight. This review focuses on the studies reported over the last decade where peptide-loaded or peptide-derived hydrogels have been tested in vivo for topical treatment of diabetic wounds and further explores the potential of peptidomimetics to expand the portfolio of hydrogel-based treatments for diabetic foot ulcers.

糖尿病足溃疡是糖尿病的主要并发症，也是一种特别复杂的慢性伤口，因为大多数糖尿病相关的生理失调会损害愈合并增加感染的风险。尽管为促进糖尿病足溃疡的预防和适当管理采取了许多卫生扫盲举措，但这仍然是一个严重的保健问题，其发病率正在上升。这不仅要求在医疗机构中不断更新适当干预的指导方针，而且要求新的局部治疗能够改善患者的健康和福祉。在后一种情况下，目前的选择包括局部氧疗、负压伤口治疗、不同类型的伤口敷料和局部凝胶。由于水凝胶基敷料在糖尿病伤口治疗中的巨大潜力，人们已经探索了几种天然或合成的水凝胶。水凝胶聚合物基质可以装载许多不同的分子（货物），最终结合不同的治疗作用。在这种情况下，肽正在获得相关性，这不仅限于那些用作生物活性货物的肽，因为能够形成水凝胶的自组装肽也在聚光灯下。这篇综述的重点是在过去的十年中，肽负载或肽衍生的水凝胶已经在体内测试用于局部治疗糖尿病伤口的研究，并进一步探讨了拟肽的潜力，以扩大水凝胶治疗糖尿病足溃疡的组合。

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引用次数: 0

Machine learning-based identification of elite genotypes in the endangered Nilgirianthus ciliatus through qualitative and quantitative trait analysis 基于定性和定量性状分析的濒危纤毛Nilgirianthus ciliatus优秀基因型的机器学习鉴定

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 Epub Date: 2025-06-26 DOI: 10.1016/j.crbiot.2025.100307

Pavan K. Kumar , Collince Omondi Awere , Anitha R. Kumari , Andaç Batur Çolak , Mustafa Bayrak , Fredrick Otieno Ogolla , Suresh Govindan , Manikandan Ramesh

Nilgirianthus ciliatus is an economically valuable endangered medicinal plant with a significant influence on traditional medicine and Ayurveda formulation. Its rarity in natural habitats precludes scientific investigation into its potential medicinal and other industrial applications. The current study examined the qualitative, quantitative and machine learning (ML) predictions for the identification of elite genotypes of N. ciliatus in India’s Western Ghats. The gas chromatography-mass spectroscopy (GC–MS) revealed the presence of betazole, neophytadiene, hexadecanoic acid methyl ester, octadecanoic acid, and squalene. The genotype NC 10 was found to yield high squalene content (793.0 ng), while the highest α-glucosidase Inhibitory Activity was shown by NC 2. The artificial neural network (ANN) demonstrated a high prediction accuracy (MSE value = 2.43E-02 while R value = 0.99992) in both the training and the testing sets of data. Genetic markers produced 140 bands, out of which 115 were polymorphic (82.14 %). Further, NC 10, NC 8, and NC 6 elite genotypes of N. ciliatus from three distinct agroclimatic zones were commended as industrially significant high-yielding characteristics and determined to be best suitable for cultivation. This study would serve as a foundation for understanding the use of artificial neural networks in elite genotype selection for efficient secondary metabolite synthesis.

Nilgirianthus ciliatus是一种具有经济价值的濒危药用植物，对传统医药和阿育吠陀配方具有重要影响。它在自然栖息地的稀缺性妨碍了对其潜在药用和其他工业应用的科学研究。目前的研究检查了定性、定量和机器学习（ML）预测，以鉴定印度西高止山脉的纤毛螨的精英基因型。气相色谱-质谱（GC-MS）分析结果显示，样品中含有倍唑、新植物二烯、十六烷酸甲酯、十八烷酸和角鲨烯。结果表明，基因型NC 10的角鲨烯含量最高（793.0 ng），而基因型NC 2的α-葡萄糖苷酶抑制活性最高。人工神经网络（ANN）在训练集和测试集的预测准确率均较高（MSE = 2.43E-02, R = 0.99992）。遗传标记共产生140个条带，其中多态性115个（82.14%）。此外，来自三个不同农业气候带的纤毛螨的NC 10、NC 8和NC 6优良基因型被认为具有工业上显著的高产特性，并确定为最适合种植的品种。该研究将为理解人工神经网络在高效次级代谢物合成的精英基因型选择中的应用奠定基础。

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引用次数: 0

Construction of targeting GPR77+CD10+ lipid nanoparticles and validation of targeting capability in vitro and in vivo 靶向GPR77+CD10+脂质纳米颗粒的构建及体外和体内靶向能力的验证

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 Epub Date: 2025-04-19 DOI: 10.1016/j.crbiot.2025.100291

Junyue Fang , Qiongchao Jiang , Xinyu Yang , Weifan Li , Li Lin , Meng Zhang , Phei Er Saw , Xiaoyun Xiao

Dual-receptor targeting strategies hold promise for improving nanocarrier specificity in complex tumor microenvironments. Herein, we engineered lipid nanoparticles (LNPs) functionalized with GPR77 and CD10 antibodies to exploit receptor co-expression as a mechanism for enhanced targeting. To rigorously validate this approach, we developed a GPR77⁺CD10⁺ overexpressing CHO cell model, which served as a controlled system to dissect ligand-receptor interactions. The dual-targeting LNPs (DOPE(GPR77/CD10)) exhibited significantly higher cellular uptake in receptor-positive CHO cells compared to single-targeted or non-targeted formulations, demonstrating synergistic binding efficacy. These LNPs also showed excellent drug encapsulation and prolonged circulation. In a CHO xenograft model, dual-targeting LNPs achieved higher tumor accumulation than non-targeted controls, with minimal off-target organ retention. Biosafety assessments confirmed negligible hemolysis and no hepatorenal toxicity. While this study focused on mechanistic validation in a simplified model, our findings establish a generalizable platform for dual-receptor targeting, with potential applications in stromal or tumor cell-specific drug delivery.

双受体靶向策略有望提高复杂肿瘤微环境中纳米载体的特异性。在此，我们设计了具有GPR77和CD10抗体功能化的脂质纳米颗粒（LNPs），以利用受体共表达作为增强靶向的机制。为了严格验证这种方法，我们开发了GPR77+CD10+过表达CHO细胞模型，作为一个受控系统来解剖配体-受体相互作用。与单靶向或非靶向制剂相比，双靶向LNPs （DOPE(GPR77/CD10)）在受体阳性的CHO细胞中表现出显著更高的细胞摄取，显示出协同结合效果。这些LNPs还具有良好的药物包被性和长循环性。在CHO异种移植模型中，双靶向LNPs比非靶向对照实现了更高的肿瘤积累，并具有最小的脱靶器官保留。生物安全评估证实可忽略溶血，无肝肾毒性。虽然这项研究的重点是在简化模型中进行机制验证，但我们的发现为双受体靶向建立了一个可推广的平台，在基质或肿瘤细胞特异性药物递送中具有潜在的应用前景。

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引用次数: 0

Preliminary evaluation of the anti-acne potential of herbal and fruit extracts and formulated creams 抗痤疮潜力的初步评估草药和水果提取物和配方面霜

IF 4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Current Research in Biotechnology

Pub Date : 2025-01-01 Epub Date: 2025-10-10 DOI: 10.1016/j.crbiot.2025.100339

Benish Gulzar , Muniba Ahmad , Dilara Abbas Bukhari , Zuhra Bibi , Arif Ullah , Abdul Rehman

The objective of the research was to evaluate the antibacterial efficacy of the selected herbal and fruit extracts against acne-causing bacteria and then to prepare effective anti-acne creams. People aged 15 to 19 were selected for taking acne samples, and bacterial isolates were characterized through biochemical methods (Gram staining, catalase, glucose fermentation) initially, and finally through a molecular approach. Six strains were identified through molecular characterisation, which included DNA extraction, polymerase chain reaction, and 16S rRNA gene sequencing: S1 (Staphylococcus sp.), S2 (Cutibacterium acnes), S3 (Staphylococcus aureus), S4 (Staphylococcus epidermis), S5 (Bacillus paramycoides), and S6 (Mammalicoccus lentus). Ethanolic extracts of herbs, i.e., neem, tulsi, liquorice, turmeric, amaltas, and fruits, i.e., sweet lime, banana, pomegranate, and lemon, were made and added to cream formulations. The antibacterial activity of the herbal and fruit extracts was analysed by well and disc diffusion methods, whereas phytochemical components of the extracts were determined throughanalysed by using GC–MS analysis. The extracts of neem and pomegranate are the most effective ones among all the tested strains, which were confirmed by significant inhibition zones and ANOVA analysis. Notably, GC–MS analysis detected the presence of key bioactive compounds such as mahanimbine in neem and hydroxymethylfurfural in pomegranate, which have anti-inflammatory and antibacterial effects relevant to treating acne. On the other hand, liquorice demonstrated the least efficacy. The neem, tulsi, liquorice, and other herbal extracts-based cream (Formulation A) had considerably high zones of inhibition, especially 37.67 mm in the case of Staphylococcus sp., and 37.66 mm in the case of C. acnes. Comparatively, moderate antibacterial activity was displayed by fruit-based cream (Formulation B) with the largest inhibition range of 25 and 22 mm towards C. acnes and B. paramycoides, respectively. The results also confirm the possible use of certain plant-based compositions in acne treatment, and they should be used in future in vivo studies to prove clinical effectiveness.

本研究的目的是评价所选草药和水果提取物对引起痤疮的细菌的抑菌效果，然后制备有效的抗痤疮霜。选取年龄在15 ~ 19岁的人群采集痤疮样本，通过革兰氏染色、过氧化氢酶、葡萄糖发酵等生化方法对分离的细菌进行初步鉴定，最后通过分子方法对分离的细菌进行鉴定。通过DNA提取、聚合酶链反应、16S rRNA基因测序等方法鉴定出6株菌株：S1（葡萄球菌）、S2（痤疮角质杆菌）、S3（金黄色葡萄球菌）、S4（表皮葡萄球菌）、S5（副芽孢杆菌）、S6（乳球菌）。草药的乙醇提取物，如楝树、土尔西、甘草、姜黄、阿玛尔塔和水果，如甜酸橙、香蕉、石榴和柠檬，被制成并添加到奶油配方中。采用孔扩散法和圆盘扩散法分析了中药和水果提取物的抑菌活性，采用气相色谱-质谱法测定了提取物的植物化学成分。通过显著的抑菌区和方差分析证实，楝树和石榴提取物的抑菌效果最好。值得注意的是，GC-MS分析检测到楝树中的mahanimbine和石榴中的羟甲基糠醛等关键生物活性化合物的存在，这些化合物具有抗炎和抗菌作用，与治疗痤疮有关。另一方面，甘草的效果最差。楝树、杜尔丝、甘草和其他草本提取物为基础的乳膏（配方A）具有相当高的抑制区，特别是对葡萄球菌的抑制区为37.67 mm，对痤疮C.的抑制区为37.66 mm。相比之下，水果乳膏（配方B）的抑菌活性中等，对痤疮芽孢杆菌和副芽孢杆菌的抑菌范围最大，分别为25和22 mm。研究结果还证实了某些植物成分可能用于治疗痤疮，并且应该在未来的体内研究中使用它们来证明临床有效性。

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