Current Research in Biotechnology最新文献

{"title":"Exploring the phytochemical composition and the anticancer potential of Haliclona fascigera and Stylissa carteri Red Sea sponges in colon cancer treatment: A promising approach","authors":"Samah S. Abuzahrah ,&nbsp;Nouf Juaid ,&nbsp;Tahani Bakhsh ,&nbsp;Serag Eldin I. Elbehairi ,&nbsp;Mohammed A. Baghdadi ,&nbsp;Reham Hassan Mekky","doi":"10.1016/j.crbiot.2025.100331","DOIUrl":"10.1016/j.crbiot.2025.100331","url":null,"abstract":"<div><div>This study investigates the phytochemical composition and anticancer properties of extracts from the marine sponges <em>Haliclona fascigera (HFE)</em> and <em>Stylissa carteri</em> (<em>SCE)</em>, collected from the Red Sea coast of Jeddah, Saudi Arabia, against human colorectal cancer cell lines HCT116 and HT-29 employing SRB assay at varying concentrations (ranging from 0.01 to 1000 µg/mL), compared to the positive control doxorubicin (DOX). The RP-HPLC-QTOF-MS and tandem MS/MS revealed the presence of 59 metabolites belonging to several classes that were described for the first time in <em>HFE</em> and <em>SCE</em> with a predominance of nitrogenous compounds. <em>SCE</em> exhibited a markedly more pronounced cytotoxic effect, with IC₅₀ of 1.9 ± 0.04 µg/mL for the HCT-116 cell line and 7.8 ± 0.6 µg/mL for the HT-29 cell line. In contrast, <em>HFE</em> demonstrated higher IC₅₀ values of 46.5 ± 1.3 µg/mL for HCT-116 and 61.9 ± 2.2 µg/mL for HT-29, indicating that <em>SCE</em> is significantly more potent than <em>HFE</em>, particularly in the HCT-116 cell line. Cell cycle analysis indicated that <em>SCE</em> predominantly induces apoptosis in the S-phase, while <em>HFE</em> significantly affects the G2-phase. Flow cytometric analysis demonstrated late apoptosis in both cell lines, with <em>HFE</em> inducing late apoptosis in 88.72 % of HCT-116 cells and 90.50 % of HT-29 cells, while <em>SCE</em> induced late apoptosis in 58.36 % of HCT-116 cells and 72.22 % of HT-29 cells. Treatment with <em>HFE</em> and <em>SCE</em> led to significant downregulation of the anti-apoptotic gene Bcl-Xl in both HCT116 and HT-29 cells, with <em>HFE</em> showing the strongest effect, especially in HT-29 cells (0.10-fold change). For the pro-apoptotic p53 gene, both extracts increased expression levels, with <em>HFE</em> yielding the highest upregulation in HCT116 cells (1.51-fold change). This suggests that these sponge extracts promote apoptosis by decreasing anti-apoptotic signalling while enhancing pro-apoptotic pathways, potentially aiding in cancer cell viability reduction.The study evaluates the binding affinities of various compounds against Bcl-2, Human Survivin, and KRAS p.G12C, which are key targets in colorectal cancer. Eicosanamide exhibited the highest binding affinities, outperforming both the co-crystallized ligands and Doxorubicin in several cases, making it a promising candidate for further investigation. Hyptolide and Palmitic Acid also showed significant potential, especially against KRAS p.G12C and Bcl-2, indicating their potential as multi-target inhibitors in colorectal cancer therapy. These findings indicate that both sponge extracts possess promising anticancer properties, with <em>SCE</em> being a particularly potent candidate for further investigation and potential therapeutic applications.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100331"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145009950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomics of the microbial consortium in the anaerobic co-digestion of food waste and poultry droppings","authors":"Oluwatomisin A. Akinsola ,&nbsp;Samuel O. Dahunsi ,&nbsp;Ebenezer L. Odekanle ,&nbsp;Olajire D. Adedokun","doi":"10.1016/j.crbiot.2025.100328","DOIUrl":"10.1016/j.crbiot.2025.100328","url":null,"abstract":"<div><div>The anaerobic co-digestion of food waste and poultry droppings presents a valuable strategy for achieving sustainable waste management and generating renewable energy. In this study, metagenomic analysis was utilized to investigate the microbial consortium driving the process, with a particular focus on the functional potential and interactions among microbial populations. DNA was extracted from the samples collected from the digester. The16S rRNA sequencing was used for the taxonomic diversity and functional profiling of the microbial community derived from the anaerobic digester. The findings reveal a complex interplay of diverse microbial taxa, predominantly <em>Firmicutes,</em> and <em>Bacteroidetes</em>, which are crucial for the degradation of organic matter and biogas production. The metabolic pathways utilized by the consortium were highlighted. The application of metagenomic tools, such as QIIME2 for data cleaning, BV-BRC for functional annotation, and PICRUSt for metabolic pathway analysis, facilitated a comprehensive functional profiling of the community, allowing the assessment of the contributions of individual taxa to the overall metabolic processes. The results contribute to the growing body of knowledge on microbial ecology in waste treatment systems and pave the way for future research aimed at optimizing anaerobic digestion processes for sustainable energy production.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100328"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota modulation and health benefits of various fasting regimens","authors":"Sumel Ashique ,&nbsp;Biplab Debnath ,&nbsp;Naheed Mojgani ,&nbsp;Mohd. Tariq ,&nbsp;Tanweer Haider ,&nbsp;Eman Shorog ,&nbsp;Sabina Yasmin ,&nbsp;Anas Islam ,&nbsp;Himanshu Sharma ,&nbsp;Md Sadique Hussain ,&nbsp;Ayush Madan ,&nbsp;Tabarak Malik ,&nbsp;Mohammad Yousuf Ansari","doi":"10.1016/j.crbiot.2025.100311","DOIUrl":"10.1016/j.crbiot.2025.100311","url":null,"abstract":"<div><div>Fasting is a diet intervention that has been shown to significantly modulate the gut microbiota, with potential health benefits. This review examines the impact of various fasting regimens, including time-restricted eating (TRE), Ramadan fasting (RF), and long-term fasting, on the composition and function of the gut microbiota in both animal models and human subjects. Studies have shown that fasting can increase microbial diversity, enrich beneficial bacteria such as <em>Faecalibacterium prausnitzii, Roseburia</em> spp.<em>, Akkermansia muciniphila</em>, and other Lachnospiraceae, and enhance the production of short-chain fatty acids (SCFA) such as butyrate. These microbiota changes are associated with improvements in metabolic health markers, including body weight, blood pressure, glucose regulation, and lipid profiles, as well as reduced inflammation. However, the persistence of these effects after fasting varies between studies. Dietary changes during fasting, such as meal timing and composition, play a crucial role in shaping the response of the gut microbiota.Although most studies report beneficial results, heterogeneity in results highlights the need for more research to elucidate the relative contributions of factors such as age, baseline diet, habitual microbiota composition, fasting duration, and caloric content. The mechanistic links between specific microbial metabolites and clinical endpoints remain limited, and evidence for the synergistic effects of probiotics with fasting in humans is inconclusive. Future studies should employ standardized fasting protocols, integrate multi-omics approaches, and extend follow-up periods to determine the durability of microbiota and metabolic shifts. Developing personalized fasting strategies tailored to an individual’s baseline microbiota and metabolic phenotype may optimize the therapeutic potential of fasting for gut and metabolic health.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100311"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144563554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating functional biochar and synthetic microbial consortia for circular bioeconomy and sustainable contaminant remediation","authors":"Abhishek Dadhich ,&nbsp;Rashmi Choudhary ,&nbsp;Yoshita Sharma ,&nbsp;Irra Dhar ,&nbsp;Rohit Jain","doi":"10.1016/j.crbiot.2025.100319","DOIUrl":"10.1016/j.crbiot.2025.100319","url":null,"abstract":"<div><div>Biochar, a carbon-rich byproduct of biomass pyrolysis, has emerged as a promising tool for sustainable agriculture due to its capacity to enhance soil health and support microbial interactions. Despite its well-documented role in improving nutrient cycling and acting as a soil amendment, limited attention has been given to its synergistic effects with plant probiotics. This review synthesizes current findings on biochar-mediated microbial interactions and their influence on soil fertility, nutrient bioavailability, and crop productivity. Biochar’s unique physicochemical characteristicssuch as high porosity, surface functional groups, and adsorption capacityfacilitate microbial colonization, promote enzymatic activities, and support beneficial processes like nitrogen fixation, phosphorus solubilization, and organic matter decomposition. These interactions contribute not only to improved plant growth but also to reduced greenhouse gas emissions and heavy metal toxicity. The review further highlights mechanisms like microbial immobilization and enhanced microbial resilience under stress conditions, positioning biochar as a bio-stimulant for promoting soil microbial efficiency. By integrating evidence from recent studies, this work underscores the importance of optimizing biochar formulations to suit specific microbial communities and environmental contexts. Such optimization can be achieved by tailoring pyrolysis temperatures, feedstock types, and surface functionalization to match the metabolic requirements and stress tolerance of target microbes in given agroecological zones. Ultimately, leveraging biochar–microbe synergy presents a viable strategy for enhancing agroecosystem resilience and advancing sustainable agricultural practices.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100319"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144763769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potassium sorbate enhancement of Chlorella sorokiniana cultivation: initial bacterial control and direct growth promotion in piggery wastewater","authors":"He-I Lin ,&nbsp;Chao-Hsien Lin ,&nbsp;Zi-Yan Xu ,&nbsp;Tsung-Meng Wu ,&nbsp;Pearl Pei-Chun Chang","doi":"10.1016/j.crbiot.2025.100326","DOIUrl":"10.1016/j.crbiot.2025.100326","url":null,"abstract":"<div><div>Piggery wastewater (PWW) presents environmental challenges due to high nitrogen and phosphorus content yet offers opportunities for sustainable bioenergy production through microalgal cultivation. However, indigenous microorganisms often inhibit microalgal growth during initial establishment phases. This study investigated potassium sorbate (PS) as a novel dual-function additive that provides both transient antimicrobial protection and direct metabolic enhancement for <em>Chlorella sorokiniana</em> T89 cultivation in PWW, distinguishing it from conventional antimicrobial-only strategies. PS effects were compared with sodium hypochlorite (SHC) and untreated controls, monitoring bacterial populations, algal growth, nutrient removal efficiency, and lipid productivity. PS at 1.5 g/L initially reduced bacterial counts by 2 logs, though populations recovered within 24 h. Critically, <em>Chlorella</em> growth enhancement continued despite bacterial recovery, revealing the unique dual mechanism of action that extends beyond the antimicrobial suppression by PS. PS at 1.5 g/L substantially improved nitrogen removal (over 90 % vs. 76 % for SHC and 49 % for controls) and phosphorus removal (over 98 % vs. 87 % for SHC and 72 % for controls), while achieving nearly four-fold higher biomass yield with lipid content exceeding 30 %. Component testing in sterile medium confirmed that sorbic acid, rather than potassium, drove direct metabolic growth promotion, demonstrating the dual role of PS as both an antimicrobial agent and a growth-enhancing substrate. In contrast, SHC showed strong antimicrobial activity but exhibited residual toxicity that inhibited <em>Chlorella</em> growth even under sterile conditions. These findings revealed that PS has dual functionality, providing initial bacterial suppression for successful establishment while simultaneously serving as a metabolic enhancer through sorbic acid. This work demonstrates PS as a promising, low-toxicity alternative for integrated agricultural wastewater treatment and biofuel production systems.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100326"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144879192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of targeting GPR77+CD10+ lipid nanoparticles and validation of targeting capability in vitro and in vivo","authors":"Junyue Fang ,&nbsp;Qiongchao Jiang ,&nbsp;Xinyu Yang ,&nbsp;Weifan Li ,&nbsp;Li Lin ,&nbsp;Meng Zhang ,&nbsp;Phei Er Saw ,&nbsp;Xiaoyun Xiao","doi":"10.1016/j.crbiot.2025.100291","DOIUrl":"10.1016/j.crbiot.2025.100291","url":null,"abstract":"<div><div>Dual-receptor targeting strategies hold promise for improving nanocarrier specificity in complex tumor microenvironments. Herein, we engineered lipid nanoparticles (LNPs) functionalized with GPR77 and CD10 antibodies to exploit receptor co-expression as a mechanism for enhanced targeting. To rigorously validate this approach, we developed a GPR77⁺CD10⁺ overexpressing CHO cell model, which served as a controlled system to dissect ligand-receptor interactions. The dual-targeting LNPs (DOPE(GPR77/CD10)) exhibited significantly higher cellular uptake in receptor-positive CHO cells compared to single-targeted or non-targeted formulations, demonstrating synergistic binding efficacy. These LNPs also showed excellent drug encapsulation and prolonged circulation. In a CHO xenograft model, dual-targeting LNPs achieved higher tumor accumulation than non-targeted controls, with minimal off-target organ retention. Biosafety assessments confirmed negligible hemolysis and no hepatorenal toxicity. While this study focused on mechanistic validation in a simplified model, our findings establish a generalizable platform for dual-receptor targeting, with potential applications in stromal or tumor cell-specific drug delivery.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"9 ","pages":"Article 100291"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the role of perineural invasion in cancer-associated pain: Insights and treatment strategies","authors":"Martina Catalano ,&nbsp;Lorenzo Landini ,&nbsp;Filippo Nozzoli ,&nbsp;Romina Nassini ,&nbsp;Giandomenico Roviello ,&nbsp;Francesco De Logu","doi":"10.1016/j.crbiot.2025.100305","DOIUrl":"10.1016/j.crbiot.2025.100305","url":null,"abstract":"<div><div>Perineural invasion (PNI) occurs when cancer cells infiltrate the space around nerves, commonly seen in head and neck, pancreatic, prostate, and colorectal cancers. PNI is clinically significant as it promotes metastasis, recurrence, and reduced survival. Mechanistically, it involves tumor-neural microenvironment interactions mediated by neurotrophic factors, inflammatory signals, and immune responses. PNI also causes severe neuropathic pain due to nerve compression, plasticity, and inflammation, resulting in sensory abnormalities from neuronal hyperexcitability, demyelination, and upregulated ion channels. This pain significantly impacts quality of life and complicates cancer management. Cells like macrophages and Schwann cells amplify pain via paracrine signaling and pro-inflammatory mediators. Standard pain management, including NSAIDs and opioids, often fails due to toxicity. Emerging therapies target PNI-specific mechanisms to limit tumor-nerve interactions and relieve pain. Research focuses on improving PNI models, dissecting tumor neuroimmune networks, and translating targeted therapies for effective pain relief.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100305"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144312492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-based identification of elite genotypes in the endangered Nilgirianthus ciliatus through qualitative and quantitative trait analysis","authors":"Pavan K. Kumar ,&nbsp;Collince Omondi Awere ,&nbsp;Anitha R. Kumari ,&nbsp;Andaç Batur Çolak ,&nbsp;Mustafa Bayrak ,&nbsp;Fredrick Otieno Ogolla ,&nbsp;Suresh Govindan ,&nbsp;Manikandan Ramesh","doi":"10.1016/j.crbiot.2025.100307","DOIUrl":"10.1016/j.crbiot.2025.100307","url":null,"abstract":"<div><div><em>Nilgirianthus ciliatus</em> is an economically valuable endangered medicinal plant with a significant influence<!--> <!-->on traditional medicine and Ayurveda formulation. Its rarity in natural habitats precludes scientific investigation into its potential medicinal and other industrial applications. The current study examined the qualitative, quantitative and machine learning (ML) predictions for the identification of elite genotypes of <em>N. ciliatus</em> in India’s Western Ghats<em>.</em> The gas chromatography-mass spectroscopy (GC–MS) revealed the presence of<!--> <!-->betazole, neophytadiene, hexadecanoic acid methyl ester, octadecanoic acid, and squalene. The genotype NC 10 was found to yield high<!--> <!-->squalene<!--> <!-->content (793.0 ng), while the highest α-glucosidase Inhibitory Activity was shown by NC 2. The artificial neural network (ANN) demonstrated a high prediction accuracy (MSE value = 2.43E-02 while R value = 0.99992) in both the training and the testing sets of data. Genetic markers produced 140 bands, out of which 115 were polymorphic (82.14 %). Further, NC 10, NC 8, and NC 6 elite genotypes of <em>N. ciliatus</em> from three distinct agroclimatic zones were commended as industrially significant high-yielding characteristics and determined to be best suitable for cultivation. This study would serve as a foundation for understanding the use of artificial neural networks in elite genotype selection for efficient secondary metabolite synthesis.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100307"},"PeriodicalIF":3.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovations and strategies for sustainable wastewater-integrated microalgal biodiesel production","authors":"Sonali Biswas ,&nbsp;L. Muruganandam ,&nbsp;I. Ganesh Moorthy","doi":"10.1016/j.crbiot.2025.100335","DOIUrl":"10.1016/j.crbiot.2025.100335","url":null,"abstract":"<div><div>The increasing need for developing a sustainable and renewable fuel has been one of the prime concerns in the world over the past few decades. This review proposes that microalgae have the ability to be scalable, cost efficient, eco-friendly and sustainable for biodiesel production simultaneous contributing to the treatment of wastewater. This integrated framework highlights new business opportunities for energy, wastewater, and bioproduct industries, where cost reductions from the current US $4–6 per liter to projected values near US $1.5 per liter could make microalgal biodiesel increasingly competitive with fossil fuels that can be sufficient for large scale industrial application. This review synthesizes progress in cultivation strategies, lipid extraction methods, and scale-up innovations, while outlining future directions such as wastewater-driven strain optimization, development of hybrid cultivation systems, and resolution of techno-economic and policy barriers essential for industrial deployment. Ultimately this review focuses on an array of approaches to accomplish reasonably priced, eco-friendly and sustainable way to produce biodiesel from microalgae and reduce the greenhouse effect based on critical analysis.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100335"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145094602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and kinetic analysis of Vc1509, a NAD+ dependent deacetylase from Vibrio cholerae","authors":"Arindam Jana ,&nbsp;Pragyan Mishra ,&nbsp;Ritu Ghosh ,&nbsp;Ajit Kumar Dhal ,&nbsp;Shibangini Beura ,&nbsp;Amrita Mishra ,&nbsp;Rahul Modak","doi":"10.1016/j.crbiot.2025.100320","DOIUrl":"10.1016/j.crbiot.2025.100320","url":null,"abstract":"<div><div>Protein lysine acetyltransferase and deacetylases play crucial role in the regulation of bacterial metabolism, stress response and virulence. Among deacetylases, sirtuin-type NAD⁺ dependent deacetylases, CobB, play central role in bacterial homeostasis. CobB has been extensively studied in several bacterial species like <em>E. coli, Salmonella</em> spp.<em>, Vibrio parahaemolyticus, Mycobacterium</em> etc. A CobB homolog, Vc1509, has been identified among the multiple putative deacetylases present in <em>Vibrio cholerae</em> genome. Knockout of Vc1509 led to global hyperacetylation, but there was no further biochemical characterization of enzyme activity. Our previous study reported the involvement of <em>Vibrio parahaemolyticus</em> CobB in the deacetylation of mammalian histones during host-pathogen interactions. In this study, we present the first comprehensive functional and kinetic analysis of Vc1509. We established a scalable expression system for high-yield production of recombinant Vc1509 required for detailed characterization. Vc1509 is a monomeric protein and harbours conserved secondary structure features of CobB. Our data show that Vc1509 is an active NAD⁺ dependent lysine de-acylase capable of targeting a broad range of acylated substrates, providing new insights into the enzymatic properties and substrate specificity of CobB bacterial deacetylases.</div></div>","PeriodicalId":52676,"journal":{"name":"Current Research in Biotechnology","volume":"10 ","pages":"Article 100320"},"PeriodicalIF":4.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144770641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}