Pub Date : 2019-02-09DOI: 10.20473/MOG.V26I32018.100-106
Desak Ketut Ayu Aryani, B. Prasetyo, A. Widyawaruyanti, W. Widjiati
Objective: To compare the administration of sambiloto tablets (AS201-01) and dihydroartemisinin-piperaquine phosphate tablets in pregnant mice infected by P. berghei on the expression of MIF in the placenta.Materials and Methods: Experimental laboratory research, 24 pregnant mice were divided into 4 groups with randomization, ie. non-infected, placebo, sambiloto (AS201-01) and DHP groups. On day 9 P. berghei was infected, on day 11 the treatment was given, day 15 the surgery was performed, placental samples were taken, immunohistochemical staining was given, and MIF expression was assessed.Results: The expression of MIF in the group not infected with P. berghei had the lowest mean, while the highest mean was found in the placebo group. Uninfected groups were not significantly different from sambiloto (AS201-01) tablet group. Sambiloto tablet group (AS201-01) had lower MIF expression than DHP group, but it was not significantly different. Sambiloto tablet group (AS201-01) showed lower MIF expression than placebo. MIF expression in DHP group was lower than that in placebo group. From the lowest, the MIF expressions were as follows: group not infected with P. berghei, group receiving sambiloto (AS201-01) tablet, DHP group and placebo group.Conclusion: MIF expression in the placenta of pregnant mice infected with P. berghei and receiving sambiloto (AS20-01) tablets was not different from those receiving DHP tablets.
{"title":"Comparison of the effect of sambiloto (AS 201-01) tablet and dihydroartemisinin-piperaquine on macrophage MIF expression in mice placenta infected with Plasmodium berghei","authors":"Desak Ketut Ayu Aryani, B. Prasetyo, A. Widyawaruyanti, W. Widjiati","doi":"10.20473/MOG.V26I32018.100-106","DOIUrl":"https://doi.org/10.20473/MOG.V26I32018.100-106","url":null,"abstract":"Objective: To compare the administration of sambiloto tablets (AS201-01) and dihydroartemisinin-piperaquine phosphate tablets in pregnant mice infected by P. berghei on the expression of MIF in the placenta.Materials and Methods: Experimental laboratory research, 24 pregnant mice were divided into 4 groups with randomization, ie. non-infected, placebo, sambiloto (AS201-01) and DHP groups. On day 9 P. berghei was infected, on day 11 the treatment was given, day 15 the surgery was performed, placental samples were taken, immunohistochemical staining was given, and MIF expression was assessed.Results: The expression of MIF in the group not infected with P. berghei had the lowest mean, while the highest mean was found in the placebo group. Uninfected groups were not significantly different from sambiloto (AS201-01) tablet group. Sambiloto tablet group (AS201-01) had lower MIF expression than DHP group, but it was not significantly different. Sambiloto tablet group (AS201-01) showed lower MIF expression than placebo. MIF expression in DHP group was lower than that in placebo group. From the lowest, the MIF expressions were as follows: group not infected with P. berghei, group receiving sambiloto (AS201-01) tablet, DHP group and placebo group.Conclusion: MIF expression in the placenta of pregnant mice infected with P. berghei and receiving sambiloto (AS20-01) tablets was not different from those receiving DHP tablets.","PeriodicalId":52893,"journal":{"name":"Majalah Obstetri dan Ginekologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42104814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-09DOI: 10.20473/MOG.V26I32018.107-111
Ratriana Via Parasti, W. Widjiati, S. Dwiningsih
Objectives: To determine the effect of bone marrow mesenchy-mal stem cells (BMSCs) on BMP-15 expression and Graafian follicle count in endometriosis mice.Material and Methods: This study was a laboratory randomized clinical trial on Mus musculus. The object of the study was 42 mice which were divided into 3 groups, the control, endome-triosis, and endometriosis + BMSCs groups. Comparison of BMP-15 expression and Graafian follicle count between groups was evaluated.Results: Immunohistochemical analysis showed that BMP-15 expression in control, endometriosis, and endometriosis + BMSCs groups had p=0.551, p=0.446 and p=0.917 with ANOVA test p=0.273, indicating no statistically significant differences between groups . Graafian follicular count in the three groups had p=0.31, p=0.001, and p=0.006, with the Kruskal-Wallis test p=0.001. Graafian follicles in the endometriosis + BMSCs group were higher than those in control and endometriosis groups.Conclusion:In the endometriosis mouse model with bone mar-row stem cell transplantation the BMP-15 expression in each group did not show a difference, but a significant difference was found in the number of Graafian follicles.
{"title":"Effect of bone marrow mesenchymal stem cells transplantation on BMP-15 expression and Graafian follicle count in mice model of endometriosis","authors":"Ratriana Via Parasti, W. Widjiati, S. Dwiningsih","doi":"10.20473/MOG.V26I32018.107-111","DOIUrl":"https://doi.org/10.20473/MOG.V26I32018.107-111","url":null,"abstract":"Objectives: To determine the effect of bone marrow mesenchy-mal stem cells (BMSCs) on BMP-15 expression and Graafian follicle count in endometriosis mice.Material and Methods: This study was a laboratory randomized clinical trial on Mus musculus. The object of the study was 42 mice which were divided into 3 groups, the control, endome-triosis, and endometriosis + BMSCs groups. Comparison of BMP-15 expression and Graafian follicle count between groups was evaluated.Results: Immunohistochemical analysis showed that BMP-15 expression in control, endometriosis, and endometriosis + BMSCs groups had p=0.551, p=0.446 and p=0.917 with ANOVA test p=0.273, indicating no statistically significant differences between groups . Graafian follicular count in the three groups had p=0.31, p=0.001, and p=0.006, with the Kruskal-Wallis test p=0.001. Graafian follicles in the endometriosis + BMSCs group were higher than those in control and endometriosis groups.Conclusion:In the endometriosis mouse model with bone mar-row stem cell transplantation the BMP-15 expression in each group did not show a difference, but a significant difference was found in the number of Graafian follicles. ","PeriodicalId":52893,"journal":{"name":"Majalah Obstetri dan Ginekologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48828123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-02-09DOI: 10.20473/MOG.V26I32018.123-127
I. Indrayanti, B. Rahardjo, H. Sujuti
Objectives: To determine the effect of oral cypermethrin exposure on the decrease of Bcl-2 expression in granulosa cells and antral follicle count in Rattus norvegicus ovary.Materials and Methods: This was a true experimental study using post-test control group design. This study used 24 Rattus norvegicus which were divided into 4 groups, the control group, treatment group (P1) exposed to cypermethrin 10 mg/kg BW, treatment group (P2) exposed to cypermethrin 15 mg/kg BW and treatment group (P3) exposed to cypermethrin 20 mg/kg BW. The treatment groups were exposed to cypermethrin for 28 days. A surgery was conducted in proestrus phase and ovarian organs were taken. Bcl-2 expression examination was performed by using immunohistochemical method and the antral follicle count was assessed with the Hematoxillin Eosin (HE)Results: This study showed the decrease of Bcl-2 expression in cypermethrin-exposed group compared to control group. There was a decrease in antral follicles count in cypermethrin-exposed group compared to control group.Conclusion: Cypermethrin can decrease Bcl-2 expression and antral follicles count in Rattus norvegicus ovaries.
{"title":"Effects of per oral cypermethrin exposure on Bcl-2 expression in granulose cells and antral follicle count of Rattus norvegicus ovaries","authors":"I. Indrayanti, B. Rahardjo, H. Sujuti","doi":"10.20473/MOG.V26I32018.123-127","DOIUrl":"https://doi.org/10.20473/MOG.V26I32018.123-127","url":null,"abstract":"Objectives: To determine the effect of oral cypermethrin exposure on the decrease of Bcl-2 expression in granulosa cells and antral follicle count in Rattus norvegicus ovary.Materials and Methods: This was a true experimental study using post-test control group design. This study used 24 Rattus norvegicus which were divided into 4 groups, the control group, treatment group (P1) exposed to cypermethrin 10 mg/kg BW, treatment group (P2) exposed to cypermethrin 15 mg/kg BW and treatment group (P3) exposed to cypermethrin 20 mg/kg BW. The treatment groups were exposed to cypermethrin for 28 days. A surgery was conducted in proestrus phase and ovarian organs were taken. Bcl-2 expression examination was performed by using immunohistochemical method and the antral follicle count was assessed with the Hematoxillin Eosin (HE)Results: This study showed the decrease of Bcl-2 expression in cypermethrin-exposed group compared to control group. There was a decrease in antral follicles count in cypermethrin-exposed group compared to control group.Conclusion: Cypermethrin can decrease Bcl-2 expression and antral follicles count in Rattus norvegicus ovaries.","PeriodicalId":52893,"journal":{"name":"Majalah Obstetri dan Ginekologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48548266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-23DOI: 10.20473/MOG.V26I22018.83-90
Nasrul Wahdi, W. Widjiati, A. Widyawaruyanti, B. Prasetyo
Objectives: To determine the effect of Sambiloto tablet (AS201-01) in reducing the placental Chondroitin Sulfate A (CSA) Expression of pregnant mice infected Plasmodiumberghei.Materials and Methods: Experimental study using 24 pregnant mice were divided into 4 groups with randomization. Uninfected group, the placebo group, the Sambiloto tablet (AS201-01) group and the DHP tablet (as a standart drug) group. The last three groups, were infected with P. bergheion day 9th of pregnancy, and the treatment was started at day 11th of pregnancy, and samples were terminated at day 15th of pregnancy by surgery. Placental sampling were stained with Tunnel assay to measure placental CSA antibodies.Results: The placental Chondroitin Sulfate A (CSA) expression. Uninfected group compared to Sambiloto tablet (AS201-01) groups was not significantly different (p>0.05), uninfected group compared with the other treatment groups differ meaningfully (p<0.05). Placebo group compared with all groups significantly different (p<0.05). Sambiloto tablet (AS201-01) group compared to uninfected group (p>0.05) was not significantly different, with another group was significantly different (p<0.05). DHP tablet group compared to all the groups was significantly different (p<0.05).Conclusion: Placental Chondroitin Sulfate A (CSA)expression of mice infected by Plasmodium berghei treated with Sambiloto tablet (AS201-01) lower than DHP tablet.
{"title":"The effect of sambiloto tablet (AS201-01) on placental Chondroitin Sulfate A (CSA) expression of pregnant mice infected by Plasmodium berghei","authors":"Nasrul Wahdi, W. Widjiati, A. Widyawaruyanti, B. Prasetyo","doi":"10.20473/MOG.V26I22018.83-90","DOIUrl":"https://doi.org/10.20473/MOG.V26I22018.83-90","url":null,"abstract":"Objectives: To determine the effect of Sambiloto tablet (AS201-01) in reducing the placental Chondroitin Sulfate A (CSA) Expression of pregnant mice infected Plasmodiumberghei.Materials and Methods: Experimental study using 24 pregnant mice were divided into 4 groups with randomization. Uninfected group, the placebo group, the Sambiloto tablet (AS201-01) group and the DHP tablet (as a standart drug) group. The last three groups, were infected with P. bergheion day 9th of pregnancy, and the treatment was started at day 11th of pregnancy, and samples were terminated at day 15th of pregnancy by surgery. Placental sampling were stained with Tunnel assay to measure placental CSA antibodies.Results: The placental Chondroitin Sulfate A (CSA) expression. Uninfected group compared to Sambiloto tablet (AS201-01) groups was not significantly different (p>0.05), uninfected group compared with the other treatment groups differ meaningfully (p<0.05). Placebo group compared with all groups significantly different (p<0.05). Sambiloto tablet (AS201-01) group compared to uninfected group (p>0.05) was not significantly different, with another group was significantly different (p<0.05). DHP tablet group compared to all the groups was significantly different (p<0.05).Conclusion: Placental Chondroitin Sulfate A (CSA)expression of mice infected by Plasmodium berghei treated with Sambiloto tablet (AS201-01) lower than DHP tablet. ","PeriodicalId":52893,"journal":{"name":"Majalah Obstetri dan Ginekologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42233736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-23DOI: 10.20473/MOG.V26I22018.91-97
Fulatul Anifah, D. Dasuki, K. HerlinFitriana, A. Triratnawati
Objectives: To explore the relationship of family structure and parenting style with adolescent pregnancy in SurabayaMaterials and Methods: This research uses mix methods approach with case-control design and case study. Data were collected by questionnaire and in-dept interview guidelines. This study involved 46 adolescent (23 cases and 23 control) by purpossive sampling and 6 adolescents as main participants. Quantitative data were analyzed on univariable, bivariable with chi square and multivariable with multiple logistic regression with p=0,05 and CI 95%. Qualitative data were analyzed by in-depth interviewResults: Family stucture were not statistically significant (OR 10.53; CI 0.657-168.93), but socially meaningful. There is not statistically significant between parenting style with adolescent pregnancy (OR 1.191; CI 0.373-3.806). Other factors that have a significant relationship with adolescent pregnancy is education of responden (OR 559.76; CI 3.608-23026.4). There is no statis-tically significant in education father, education mother, employment status and where they living. Parenting style can be influenced by communication between parents and adolescent.Conclusions: Risk of adolescent pregnancy on people who have low education. Adolescents from extended family and single parent have a role to the incidence of teenage pregnancy.
{"title":"Role of family structure and parenting style in adolescent pregnancy in Surabaya, Indonesia","authors":"Fulatul Anifah, D. Dasuki, K. HerlinFitriana, A. Triratnawati","doi":"10.20473/MOG.V26I22018.91-97","DOIUrl":"https://doi.org/10.20473/MOG.V26I22018.91-97","url":null,"abstract":"Objectives: To explore the relationship of family structure and parenting style with adolescent pregnancy in SurabayaMaterials and Methods: This research uses mix methods approach with case-control design and case study. Data were collected by questionnaire and in-dept interview guidelines. This study involved 46 adolescent (23 cases and 23 control) by purpossive sampling and 6 adolescents as main participants. Quantitative data were analyzed on univariable, bivariable with chi square and multivariable with multiple logistic regression with p=0,05 and CI 95%. Qualitative data were analyzed by in-depth interviewResults: Family stucture were not statistically significant (OR 10.53; CI 0.657-168.93), but socially meaningful. There is not statistically significant between parenting style with adolescent pregnancy (OR 1.191; CI 0.373-3.806). Other factors that have a significant relationship with adolescent pregnancy is education of responden (OR 559.76; CI 3.608-23026.4). There is no statis-tically significant in education father, education mother, employment status and where they living. Parenting style can be influenced by communication between parents and adolescent.Conclusions: Risk of adolescent pregnancy on people who have low education. Adolescents from extended family and single parent have a role to the incidence of teenage pregnancy.","PeriodicalId":52893,"journal":{"name":"Majalah Obstetri dan Ginekologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45280503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-23DOI: 10.20473/MOG.V26I22018.74-82
Masyitah Hamidah, A. Widyawaruyanti, W. Widjiati, B. Prasetyo
Objectives: To compare the TLR2 expression in the placenta between treated by sambiloto EA-96 fraction tablet (AS201-01) and dihydroartemisinin-piperaquine phosphate (DHP)Materials and Methods: Experimental study using 24 pregnant mice. All sample divided into 4 groups with randomization are uninfected group, Plasmodium infected group and given placebo, sambiloto (AS201-01) and DHP. Then performed surgery and placental sampling were staining with adopting in tunnel assay method to measure the TLR2 expression of placental.Results: The expression of TLR2 in uninfected group has the lowest rate compared to other groups. The infected and placebo treated group has the highest TLR2 expression campared with sambiloto and DHP. The sambiloto group has not differ signi-ficantly with the group uninfected and lower than DHP.Conclusion: Tablet of sambiloto EA-96 fraction (AS201-01) decreased TLR2 expession better than with DHP tablet.
{"title":"Sambiloto (AS201-01) is better than standard antimalarial drug (DHP) in reducing Toll-Like Receptor 2 (TLR2) on placenta malaria model","authors":"Masyitah Hamidah, A. Widyawaruyanti, W. Widjiati, B. Prasetyo","doi":"10.20473/MOG.V26I22018.74-82","DOIUrl":"https://doi.org/10.20473/MOG.V26I22018.74-82","url":null,"abstract":"Objectives: To compare the TLR2 expression in the placenta between treated by sambiloto EA-96 fraction tablet (AS201-01) and dihydroartemisinin-piperaquine phosphate (DHP)Materials and Methods: Experimental study using 24 pregnant mice. All sample divided into 4 groups with randomization are uninfected group, Plasmodium infected group and given placebo, sambiloto (AS201-01) and DHP. Then performed surgery and placental sampling were staining with adopting in tunnel assay method to measure the TLR2 expression of placental.Results: The expression of TLR2 in uninfected group has the lowest rate compared to other groups. The infected and placebo treated group has the highest TLR2 expression campared with sambiloto and DHP. The sambiloto group has not differ signi-ficantly with the group uninfected and lower than DHP.Conclusion: Tablet of sambiloto EA-96 fraction (AS201-01) decreased TLR2 expession better than with DHP tablet.","PeriodicalId":52893,"journal":{"name":"Majalah Obstetri dan Ginekologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48373115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-23DOI: 10.20473/MOG.V26I22018.55-60
A. S. Putra, Suhatno Suhatno
Objective: To know the efficacy differences between two groups of drug combination ondansetron-dexamethasone (A) and ondan-setron-dexamethasone (B) to prevent emetic rsponses after chemotherapy cisplatin administration (CINV).Materials and Methods: A prospective double blind randomized clinical trial study held in Dr. Soetomo General Hospital involving 66 subjects, devide into two groups randomly 33 patients each. One group receive palonosetron-dexamethasone combination therapy compare to ondansetron-dexamethasone combination as standart therapy in gynecologic oncology Dr. Soetomo Hospital in a control group as cisplatin chemotharapy CINV prophylaxis. The research was conducted from June till October 2014 in the Dr. Soetomo General Hospital-Surabaya. Assessment and measurement of the response of nausea and frequency of vomiting according Gralla scale, changes in plasma density and electrolyte serum (Na, K, and Cl) as a result of dehydration caused by nausea and vomiting, as well as counting the onset of nausea and vomiting occur.Results: There was significant differences between the results of the combination therapy compared ondansetron-dexamethasone to palonosetron-dexamethasone for nausea and vomiting (p=0,001). 31 subjects suffers nausea in ondansetron group, while 9 subjects suffers nausea in palonosetron group. Vomiting occurs in 17 subjects from ondansetron group, and only 1 subject from palono-setron group during study. Changes in plasma density significant in palonosetron group, and natrium in ondansetron group. For K and Cl not significantly difference in both groups before and after cisplatin chemotherapy administration.Conclusion: Palonosetron-dexamethasone combination is super-ior as cisplatin CINV prophylaxis in response to nausea and vomiting frequency, and also give longer protection compare to ondansetron-dexamethasone significantly. The plasma density and electrolyte serum changes are varied in numbers and also influenced by many factors including physical status and nutrition, also intake of each patients.
{"title":"Combination of palonosetron-dexamethasone is more effective than ondansetron-dexamethasone as single cisplatin antiemetic chemotherapy","authors":"A. S. Putra, Suhatno Suhatno","doi":"10.20473/MOG.V26I22018.55-60","DOIUrl":"https://doi.org/10.20473/MOG.V26I22018.55-60","url":null,"abstract":"Objective: To know the efficacy differences between two groups of drug combination ondansetron-dexamethasone (A) and ondan-setron-dexamethasone (B) to prevent emetic rsponses after chemotherapy cisplatin administration (CINV).Materials and Methods: A prospective double blind randomized clinical trial study held in Dr. Soetomo General Hospital involving 66 subjects, devide into two groups randomly 33 patients each. One group receive palonosetron-dexamethasone combination therapy compare to ondansetron-dexamethasone combination as standart therapy in gynecologic oncology Dr. Soetomo Hospital in a control group as cisplatin chemotharapy CINV prophylaxis. The research was conducted from June till October 2014 in the Dr. Soetomo General Hospital-Surabaya. Assessment and measurement of the response of nausea and frequency of vomiting according Gralla scale, changes in plasma density and electrolyte serum (Na, K, and Cl) as a result of dehydration caused by nausea and vomiting, as well as counting the onset of nausea and vomiting occur.Results: There was significant differences between the results of the combination therapy compared ondansetron-dexamethasone to palonosetron-dexamethasone for nausea and vomiting (p=0,001). 31 subjects suffers nausea in ondansetron group, while 9 subjects suffers nausea in palonosetron group. Vomiting occurs in 17 subjects from ondansetron group, and only 1 subject from palono-setron group during study. Changes in plasma density significant in palonosetron group, and natrium in ondansetron group. For K and Cl not significantly difference in both groups before and after cisplatin chemotherapy administration.Conclusion: Palonosetron-dexamethasone combination is super-ior as cisplatin CINV prophylaxis in response to nausea and vomiting frequency, and also give longer protection compare to ondansetron-dexamethasone significantly. The plasma density and electrolyte serum changes are varied in numbers and also influenced by many factors including physical status and nutrition, also intake of each patients.","PeriodicalId":52893,"journal":{"name":"Majalah Obstetri dan Ginekologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41860556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-23DOI: 10.20473/MOG.V26I22018.61-66
Menik Utami, B. Winardi
Objective: To determine the difference of the puerperial bleeding and puerperial infection women who had intrauterine contraceptive device (IUCD) T380A insertion at caesarean section compare with women who had caesarean section without IUCD insertion.Materials and Methods: This study was an observasional analytic prospective cohort. Participants allocated to 2 groups : IUCD inserted during cesarean and cesarean with no device inserted. Subject research monitoring at 10 and 40 day of puerperial period.Results: Puerperial bleeding of group with IUCD insertion is 99,309+32,845 ml and group without IUCD is 88,010+30,824 ml, with the analysis test got p=0,085 (p>0,05) means no difference between two groups.Level hemoglobin at post cesarean section (day-0), 10 and 40 day, proportion increasing severity of anemia and duration of lochia rubra were analized and got p>0,05, no difference between two groups. Duration of puerperial bleeding period, median group with IUCD 40 days (25-50) and group without IUCD 30 days (26-45), with analized test got p<0,05. Duration of puerperial bleeding were significantly difference. Proportion of clinical sign puerperial infection 3 (6,4%) of group with IUCD and 2 (4,1%) of group without IUCD, with analized test got p=0,614 (p>0,05), no difference between two groups. Level of leucocyte at post cesarean section (day-0), 10 and 40 day, the difference both two groups was not significant (p>0,05). In this research the difference puerperial bleeding and proportion puerperial infection between two groups (women who had IUCD T380A insertion at caesarean section compare with women who had caesarean section without IUCD insertion) was no significant.Conclusion: Insertion IUCD Cu T 380A postplacental at caesarean section is safety.
{"title":"Postplacental insertion of IUCD Cu T 380A at transcesarean section does not influence bleeding and infection at puerperial period","authors":"Menik Utami, B. Winardi","doi":"10.20473/MOG.V26I22018.61-66","DOIUrl":"https://doi.org/10.20473/MOG.V26I22018.61-66","url":null,"abstract":"Objective: To determine the difference of the puerperial bleeding and puerperial infection women who had intrauterine contraceptive device (IUCD) T380A insertion at caesarean section compare with women who had caesarean section without IUCD insertion.Materials and Methods: This study was an observasional analytic prospective cohort. Participants allocated to 2 groups : IUCD inserted during cesarean and cesarean with no device inserted. Subject research monitoring at 10 and 40 day of puerperial period.Results: Puerperial bleeding of group with IUCD insertion is 99,309+32,845 ml and group without IUCD is 88,010+30,824 ml, with the analysis test got p=0,085 (p>0,05) means no difference between two groups.Level hemoglobin at post cesarean section (day-0), 10 and 40 day, proportion increasing severity of anemia and duration of lochia rubra were analized and got p>0,05, no difference between two groups. Duration of puerperial bleeding period, median group with IUCD 40 days (25-50) and group without IUCD 30 days (26-45), with analized test got p<0,05. Duration of puerperial bleeding were significantly difference. Proportion of clinical sign puerperial infection 3 (6,4%) of group with IUCD and 2 (4,1%) of group without IUCD, with analized test got p=0,614 (p>0,05), no difference between two groups. Level of leucocyte at post cesarean section (day-0), 10 and 40 day, the difference both two groups was not significant (p>0,05). In this research the difference puerperial bleeding and proportion puerperial infection between two groups (women who had IUCD T380A insertion at caesarean section compare with women who had caesarean section without IUCD insertion) was no significant.Conclusion: Insertion IUCD Cu T 380A postplacental at caesarean section is safety.","PeriodicalId":52893,"journal":{"name":"Majalah Obstetri dan Ginekologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48505656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-23DOI: 10.20473/MOG.V26I22018.48-54
A. Hapsari, H. T. Joewono, W. Widjiati
Objective: To analyze the difference of neuronal apoptotic index on Rattus norvegicus offspring in cerebrum and cerebellum between food restriction 50% group and control.Materials and Methods: An analytical experimental study with single blind randomized post test only control group using animals subjects Rattus norvegicus. This study was conducted at animal laboratory, Veterinary Faculty, Universitas Airlangga. Animal subjects were divided into food restriction 50% group and control. The apoptotic index was analyzed using comparison test, with significancy p<0.05.Results: There was significant difference of neuronal apoptotic index on Rattus norvegicus offspring between FR 50% group and control in cerebrum with p= 0.002 (mean 6,12±3,51 in FR 50% group, 2,81±2,16 in control group), and cerebellum with p= 0.026 (mean 5,56±3,03 in FR group, 3,43±2,58 in control group).Conclusions: Food restriction 50% during gestation has signifi-cant influence on neuronal apoptotic index in cerebrum and cerebellum of Rattus norvegicus offspring.
{"title":"Apoptosis index of cerebrum and cerebellum neuronal cells in Rattus norvegicus neonates born from mothers treated with 50% food restriction during gestation","authors":"A. Hapsari, H. T. Joewono, W. Widjiati","doi":"10.20473/MOG.V26I22018.48-54","DOIUrl":"https://doi.org/10.20473/MOG.V26I22018.48-54","url":null,"abstract":"Objective: To analyze the difference of neuronal apoptotic index on Rattus norvegicus offspring in cerebrum and cerebellum between food restriction 50% group and control.Materials and Methods: An analytical experimental study with single blind randomized post test only control group using animals subjects Rattus norvegicus. This study was conducted at animal laboratory, Veterinary Faculty, Universitas Airlangga. Animal subjects were divided into food restriction 50% group and control. The apoptotic index was analyzed using comparison test, with significancy p<0.05.Results: There was significant difference of neuronal apoptotic index on Rattus norvegicus offspring between FR 50% group and control in cerebrum with p= 0.002 (mean 6,12±3,51 in FR 50% group, 2,81±2,16 in control group), and cerebellum with p= 0.026 (mean 5,56±3,03 in FR group, 3,43±2,58 in control group).Conclusions: Food restriction 50% during gestation has signifi-cant influence on neuronal apoptotic index in cerebrum and cerebellum of Rattus norvegicus offspring.","PeriodicalId":52893,"journal":{"name":"Majalah Obstetri dan Ginekologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41889311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-08-22DOI: 10.20473/mog.V26I12018.20-25
Hesty Widowati, H. Sujuti, Karyono Mintaroem
Objective: This study aimed to verify the effect of oral siper-metrin exposure to decrease serum estradiol 17b levels and increased malondialdehyde (MDA) in the uterus level of female Wistar strain rats (Rattus norvegicus).Materials and Methods: The method of this study was true experimental post test only control group in vivo using 24 female rats, divided into 3 groups treated by administering a dose of 5, 10 and 20 mg/kg sipermetrin for 28 days and one control group. Then blood samples were taken from the heart for measurement of serum estradiol 17b levels by ELISA and uterine organs were taken for measurement of Malondialdehyde (MDA) with spectro-photometry method.Results: The results of the measurement of serum estradiol 17b and uterus malondialdehyde (MDA) levels of female Wistar strain rats (Rattus norvegicus) showed an opposite pattern, where there was a decline in serum estradiol 17b levels and an increase in uterus malondialdehyde (MDA) level. There was a significant difference (p=0.000
{"title":"Effect of per oral sipermetrin exposure on serum 17-beta estradiol and uterine malondialdehyde (MDA) levels in female Wistar strain rats (Rattus norvegicus)","authors":"Hesty Widowati, H. Sujuti, Karyono Mintaroem","doi":"10.20473/mog.V26I12018.20-25","DOIUrl":"https://doi.org/10.20473/mog.V26I12018.20-25","url":null,"abstract":"Objective: This study aimed to verify the effect of oral siper-metrin exposure to decrease serum estradiol 17b levels and increased malondialdehyde (MDA) in the uterus level of female Wistar strain rats (Rattus norvegicus).Materials and Methods: The method of this study was true experimental post test only control group in vivo using 24 female rats, divided into 3 groups treated by administering a dose of 5, 10 and 20 mg/kg sipermetrin for 28 days and one control group. Then blood samples were taken from the heart for measurement of serum estradiol 17b levels by ELISA and uterine organs were taken for measurement of Malondialdehyde (MDA) with spectro-photometry method.Results: The results of the measurement of serum estradiol 17b and uterus malondialdehyde (MDA) levels of female Wistar strain rats (Rattus norvegicus) showed an opposite pattern, where there was a decline in serum estradiol 17b levels and an increase in uterus malondialdehyde (MDA) level. There was a significant difference (p=0.000<alpha) in 17b estradiol serum and uterus Malondialdehyde (MDA) levels of female rats between control group and group exposed to sipermetrin treatment for 28 days.Conclusions: Oral sipermetrin exposure can decrease serum levels of estradiol 17b and increase uterine levels of malondi-aldehyde (MDA) of female Wistar strain rats (Rattus norvegicus).","PeriodicalId":52893,"journal":{"name":"Majalah Obstetri dan Ginekologi","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45399767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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