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The RNA-binding domain of DCL3 is required for long-distance RNAi signaling DCL3的RNA结合域是长距离RNAi信号传导所必需的。
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-28 DOI: 10.1007/s42994-023-00124-6
Jie Li, Bo-Sen Zhang, Hua-Wei Wu, Cheng-Lan Liu, Hui-Shan Guo, Jian-Hua Zhao

Small RNA (sRNA)-mediated RNA silencing (also known as RNA interference, or RNAi) is a conserved mechanism in eukaryotes that includes RNA degradation, DNA methylation, heterochromatin formation and protein translation repression. In plants, sRNAs can move either cell-to-cell or systemically, thereby acting as mobile silencing signals to trigger noncell autonomous silencing. However, whether and what proteins are also involved in noncell autonomous silencing have not been elucidated. In this study, we utilized a previously reported inducible RNAi plant, PDSi, which can induce systemic silencing of the endogenous PDS gene, and we demonstrated that DCL3 is involved in systemic PDS silencing through its RNA binding activity. We confirmed that the C-terminus of DCL3, including the predicted RNA-binding domain, is capable of binding short RNAs. Mutations affecting RNA binding, but not processing activity, reduced systemic PDS silencing, indicating that DCL3 binding to RNAs is required for the induction of systemic silencing. Cucumber mosaic virus infection assays showed that the RNA-binding activity of DCL3 is required for antiviral RNAi in systemically noninoculated leaves. Our findings demonstrate that DCL3 acts as a signaling agent involved in noncell autonomous silencing and an antiviral effect in addition to its previously known function in the generation of 24-nucleotide sRNAs.

小 RNA(sRNA)介导的 RNA 沉默(也称为 RNA 干扰或 RNAi)是真核生物中的一种保守机制,包括 RNA 降解、DNA 甲基化、异染色质形成和蛋白质翻译抑制。在植物中,sRNA 可以在细胞间移动或系统移动,从而作为移动沉默信号触发非细胞自主沉默。然而,非细胞自主沉默中是否也有蛋白质参与以及参与的蛋白质是什么,这些问题尚未得到阐明。在本研究中,我们利用之前报道的可诱导的 RNAi 植物 PDSi,它可以诱导内源 PDS 基因的系统沉默,我们证明了 DCL3 通过其 RNA 结合活性参与了 PDS 的系统沉默。我们证实 DCL3 的 C 端(包括预测的 RNA 结合域)能够结合短 RNA。影响 RNA 结合而非处理活性的突变降低了系统性 PDS 沉默,这表明 DCL3 与 RNA 的结合是诱导系统性沉默所必需的。黄瓜花叶病毒感染试验表明,DCL3的RNA结合活性是系统非接种叶片抗病毒RNAi所必需的。我们的研究结果表明,除了之前已知的生成 24 核苷酸 sRNA 的功能外,DCL3 还是一种参与非细胞自主沉默和抗病毒作用的信号传导因子:在线版本包含补充材料,见 10.1007/s42994-023-00124-6。
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引用次数: 0
Regulation of seed traits in soybean 大豆种子性状的调控
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-27 DOI: 10.1007/s42994-023-00122-8
Yang Hu, Yue Liu, Jun-Jie Wei, Wan-Ke Zhang, Shou-Yi Chen, Jin-Song Zhang

Soybean (Glycine max) is an essential economic crop that provides vegetative oil and protein for humans, worldwide. Increasing soybean yield as well as improving seed quality is of great importance. Seed weight/size, oil and protein content are the three major traits determining seed quality, and seed weight also influences soybean yield. In recent years, the availability of soybean omics data and the development of related techniques have paved the way for better research on soybean functional genomics, providing a comprehensive understanding of gene functions. This review summarizes the regulatory genes that influence seed size/weight, oil content and protein content in soybean. We also provided a general overview of the pleiotropic effect for the genes in controlling seed traits and environmental stresses. Ultimately, it is expected that this review will be beneficial in breeding improved traits in soybean.

大豆(Glycine max)是一种重要的经济作物,为全球人类提供植物油和蛋白质。提高大豆产量和改善种子质量至关重要。种子重量/大小、油脂和蛋白质含量是决定种子质量的三大性状,种子重量也影响大豆产量。近年来,大豆组学数据的获得和相关技术的发展为更好地开展大豆功能基因组学研究铺平了道路,使人们对基因功能有了全面的了解。本综述总结了影响大豆种子大小/重量、含油量和蛋白质含量的调控基因。我们还概述了这些基因在控制种子性状和环境胁迫方面的多效应。预计本综述最终将有助于大豆改良性状的育种工作。
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引用次数: 0
Regulation of regeneration in Arabidopsis thaliana 拟南芥的再生调节。
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-22 DOI: 10.1007/s42994-023-00121-9
Md Khairul Islam, Sai Teja Mummadi, Sanzhen Liu, Hairong Wei

We employed several algorithms with high efficacy to analyze the public transcriptomic data, aiming to identify key transcription factors (TFs) that regulate regeneration in Arabidopsis thaliana. Initially, we utilized CollaborativeNet, also known as TF-Cluster, to construct a collaborative network of all TFs, which was subsequently decomposed into many subnetworks using the Triple-Link and Compound Spring Embedder (CoSE) algorithms. Functional analysis of these subnetworks led to the identification of nine subnetworks closely associated with regeneration. We further applied principal component analysis and gene ontology (GO) enrichment analysis to reduce the subnetworks from nine to three, namely subnetworks 1, 12, and 17. Searching for TF-binding sites in the promoters of the co-expressed and co-regulated (CCGs) genes of all TFs in these three subnetworks and Triple-Gene Mutual Interaction analysis of TFs in these three subnetworks with the CCGs involved in regeneration enabled us to rank the TFs in each subnetwork. Finally, six potential candidate TFs—WOX9A, LEC2, PGA37, WIP5, PEI1, and AIL1 from subnetwork 1—were identified, and their roles in somatic embryogenesis (GO:0010262) and regeneration (GO:0031099) were discussed, so were the TFs in Subnetwork 12 and 17 associated with regeneration. The TFs identified were also assessed using the CIS-BP database and Expression Atlas. Our analyses suggest some novel TFs that may have regulatory roles in regeneration and embryogenesis and provide valuable data and insights into the regulatory mechanisms related to regeneration. The tools and the procedures used here are instrumental for analyzing high-throughput transcriptomic data and advancing our understanding of the regulation of various biological processes of interest.

我们采用了几种高效算法来分析公共转录组数据,目的是找出调控拟南芥再生的关键转录因子(TFs)。最初,我们利用 CollaborativeNet(又称 TF-Cluster)构建了所有 TFs 的协作网络,随后利用 Triple-Link 和 Compound Spring Embedder(CoSE)算法将其分解为许多子网络。通过对这些子网络进行功能分析,确定了九个与再生密切相关的子网络。我们进一步应用主成分分析和基因本体(GO)富集分析,将子网络从九个减少到三个,即子网络 1、12 和 17。搜索这三个子网络中所有 TF 的共表达和共调控(CCGs)基因启动子中的 TF 结合位点,并对这三个子网络中的 TF 与参与再生的 CCGs 进行三基因互作分析,从而对每个子网络中的 TF 进行排序。最后,我们确定了子网络 1 中的六个潜在候选 TF--WOX9A、LEC2、PGA37、WIP5、PEI1 和 AIL1,并讨论了它们在体细胞胚胎发生(GO:0010262)和再生(GO:0031099)中的作用,以及子网络 12 和 17 中与再生相关的 TF。我们还利用 CIS-BP 数据库和表达图谱对所发现的 TFs 进行了评估。我们的分析提示了一些可能在再生和胚胎发生过程中具有调控作用的新型 TFs,并为了解与再生相关的调控机制提供了宝贵的数据和见解。这里使用的工具和程序有助于分析高通量转录组数据,推进我们对各种相关生物过程调控的理解:在线版本包含补充材料,可查阅 10.1007/s42994-023-00121-9。
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引用次数: 0
Staying hungry: a roadmap to harnessing central regulators of symbiotic nitrogen fixation under fluctuating nitrogen availability 保持饥饿:在氮供应波动条件下利用共生固氮中心调节器的路线图
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-11-18 DOI: 10.1007/s42994-023-00123-7
Lijin Qiao, Jieshun Lin, Takuya Suzaki, Pengbo Liang

Legumes have evolved specific inventions to enhance nitrogen (N) acquisition by establishing symbiotic interactions with N-fixing rhizobial bacteria. Because symbiotic N fixation is energetically costly, legumes have developed sophisticated mechanisms to ensure carbon–nitrogen balance, in a variable environment, both locally and at the whole plant level, by monitoring nodule number, nodule development, and nodular nitrogenase activity, as well as controlling nodule senescence. Studies of the autoregulation of nodulation and regulation of nodulation by nodule inception (NIN) and NIN-LIKE PROTEINs (NLPs) have provided great insights into the genetic mechanisms underlying the nitrate-induced regulation of root nodulation for adapting to N availability in the rhizosphere. However, many aspects of N-induced pleiotropic regulation remain to be fully explained, such as N-triggered senescence in mature nodules. Wang et al. determined that this process is governed by a transcriptional network regulated by NAC-type transcription factors. Characterization and dissection of these soybean nitrogen-associated NAPs (SNAPs) transcription factor-mastered networks have yielded a roadmap for exploring how legumes rewire nodule functions across a range of N levels, laying the foundation for enhancing the growth of N-deprived crops in agricultural settings.

豆科植物通过与固氮根瘤菌建立共生关系,进化出了一些特殊的发明来提高氮(N)的获取能力。由于共生固氮的能量成本很高,豆科植物已经发展出复杂的机制,通过监测结核数量、结核发育和结核固氮酶活性以及控制结核衰老,在多变的环境中确保局部和整个植株水平的碳氮平衡。对结核的自动调节以及结核萌发(NIN)和 NIN-LIKE PROTEINs(NLPs)对结核的调节的研究,为了解硝酸盐诱导的根瘤调节适应根瘤层中氮供应的遗传机制提供了重要见解。然而,氮诱导的多效性调控的许多方面仍有待充分解释,如成熟结核中的氮触发衰老。Wang 等人确定,这一过程受 NAC 型转录因子调控的转录网络支配。对这些大豆氮相关 NAPs(SNAPs)转录因子主控网络的表征和剖析,为探索豆科植物如何在一系列氮水平下重新连接结节功能提供了路线图,为提高农业环境中缺氮作物的生长奠定了基础。
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引用次数: 0
Tomato leaf curl New Delhi virus: an emerging plant begomovirus threatening cucurbit production 番茄卷叶新德里病毒:一种威胁瓜类生产的新兴植物begomavirus。
IF 3.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-10-25 DOI: 10.1007/s42994-023-00118-4
Lingmin Cai, Yuzhen Mei, Ruyi Ye, Yun Deng, Xuejun Zhang, Zhongyuan Hu, Xueping Zhou, Mingfang Zhang, Jinghua Yang

Tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus, was first reported to infect tomato and has recently spread rapidly as an emerging disease to Cucurbitaceae crops. To date, the virus has been reported to infect more than 11 cucurbit crops, in 16 countries and regions, causing severe yield losses. In autumn 2022, ToLCNDV was first isolated from cucurbit plants in Southeastern coastal areas of China. Phylogenetic analysis established that these isolates belong to the Asian ToLCNDV clade, and shared high nucleotide identity and closest genetic relationship with the DNA-A sequence from the Chinese tomato-infecting ToLCNDV isolate (Accession no. OP356207) and the tomato New Delhi ToLCNDV-Severe isolate (Accession no. HM159454). In this review, we summarize the occurrence and distribution, host range, detection and diagnosis, control strategies, and genetic resistance of ToLCNDV in the Cucurbitaceae. We then summarize pathways that could be undertaken to improve our understanding of this emerging disease, with the objective to develop ToLCNDV-resistant cucurbit cultivars.

番茄卷曲新德里病毒(ToLCNDV)是一种双部begomvirus,首次报道感染番茄,近年来作为一种新发病害在葫芦科作物中迅速传播。迄今为止,据报道,该病毒感染了16个国家和地区的11种以上葫芦作物,造成严重的产量损失。2022年秋季,ToLCNDV首次从中国东南沿海地区的葫芦植物中分离出来。系统发育分析表明,这些分离株属于亚洲ToLCNDV分支,与侵染ToLCNDV的中国番茄分离株的DNA-A序列具有高核苷酸同源性和最密切的遗传关系。OP356207)和番茄新德里tolcndv严重分离株(加入号:HM159454)。本文综述了ToLCNDV在葫芦科的发生分布、寄主范围、检测诊断、防治策略及遗传抗性等方面的研究进展。然后,我们总结了可以采取的途径,以提高我们对这种新出现的疾病的理解,目的是开发抗tolcndv的葫芦品种。补充信息:在线版本包含补充资料,下载地址:10.1007/s42994-023-00118-4。
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引用次数: 0
Co-expression of GR79 EPSPS and GAT generates high glyphosate-resistant alfalfa with low glyphosate residues GR79 EPSPS 和 GAT 的共表达可产生低草甘膦残留的高草甘膦抗性紫花苜蓿
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-10-19 DOI: 10.1007/s42994-023-00119-3
Yingying Meng, Wenwen Zhang, Zhaoming Wang, Feng Yuan, Sandui Guo, Hao Lin, Lifang Niu

Weed competition seriously threatens the yield of alfalfa, the most important forage legume worldwide, thus generating herbicide-resistant alfalfa varieties is becoming a necessary cost-effective strategy to assist farmers for weed control. Here, we report the co-expression of plant codon-optimized forms of GR79 EPSPS (pGR79 EPSPS) and N-acetyltransferase (pGAT) genes, in alfalfa, via Agrobacterium-mediated transformation. We established that the pGR79 EPSPS-pGAT co-expression alfalfa lines were able to tolerate up to tenfold higher commercial usage of glyphosate and produced approximately ten times lower glyphosate residues than the conventional cultivar. Our findings generate an elite herbicide-resistant germplasm for alfalfa breeding and provide a promising strategy for developing high-glyphosate-resistant and low-glyphosate-residue forages.

苜蓿是世界上最重要的饲用豆科植物,杂草的竞争严重威胁着苜蓿的产量,因此培育抗除草剂的苜蓿品种已成为帮助农民控制杂草的一种必要的经济有效的策略。在此,我们报告了通过农杆菌介导转化法在紫花苜蓿中共同表达 GR79 EPSPS(pGR79 EPSPS)和 N-乙酰转移酶(pGAT)基因的植物密码子优化形式。我们发现,pGR79 EPSPS-pGAT 基因共同表达的紫花苜蓿品系能够耐受高十倍的草甘膦商业用量,其产生的草甘膦残留量比传统栽培品种低约十倍。我们的研究结果为紫花苜蓿育种提供了抗除草剂的精英种质,并为开发高草甘膦抗性和低草甘膦残留的牧草提供了一种前景广阔的策略。
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引用次数: 0
Application of Wox2a in transformation of recalcitrant maize genotypes 将 Wox2a 应用于转化难以克服的玉米基因型
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-10-13 DOI: 10.1007/s42994-023-00116-6
Qiangbo Liu, Xian Sheng Zhang, Ying Hua Su

The genetic transformation plays an important role in plant gene functional analysis and its genetic improvement. However, only a limited number of maize germplasms can be routinely transformed. The maize gene Wuschel-like homeobox protein 2a (Wox2a) was shown to play a crucial role in promoting the formation of embryonic cells and enhancing the efficiency of genetic transformation in maize. This commentary discusses the mechanism by which the Wox2a gene contributes to the variation in embryogenic tissue culture response among different maize inbred lines. In addition, the frequency and intensity of Wox2a or Wus2/Bbm vector-induced somatic embryogenesis was also discussed. The application of Wox2a in transformation of recalcitrant maize genotypes could well accelerate the development of maize genetic improvement.

基因转化在植物基因功能分析和遗传改良中发挥着重要作用。然而,只有数量有限的玉米种质可以进行常规转化。研究表明,玉米基因 Wuschel-like homeobox protein 2a (Wox2a) 在促进玉米胚胎细胞的形成和提高遗传转化效率方面发挥着重要作用。本评论探讨了 Wox2a 基因导致不同玉米近交系胚胎组织培养反应差异的机制。此外,还讨论了 Wox2a 或 Wus2/Bbm 载体诱导体细胞胚胎发生的频率和强度。将 Wox2a 应用于转化顽固的玉米基因型可加速玉米遗传改良的发展。
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引用次数: 0
Regulation of a vacuolar proton-pumping P-ATPase MdPH5 by MdMYB73 and its role in malate accumulation and vacuolar acidification MdMYB73 对液泡质子泵 P-ATP 酶 MdPH5 的调控及其在苹果酸积累和液泡酸化中的作用
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-09-22 DOI: 10.1007/s42994-023-00115-7
Xiao-Yu Huang, Ying Xiang, Yu-Wen Zhao, Chu-Kun Wang, Jia-Hui Wang, Wen-Yan Wang, Xiao-Long Liu, Quan Sun, Da-Gang Hu

As the main organic acid in fruits, malate is produced in the cytoplasm and is then transported into the vacuole. It accumulates by vacuolar proton pumps, transporters, and channels, affecting the taste and flavor of fruits. Among the three types of proton pumps (V-ATPases, V-PPases, and P-ATPases), the P-ATPases play an important role in the transport of malate into vacuoles. In this study, the transcriptome data, collected at different stages after blooming and during storage, were analyzed and the results demonstrated that the expression of MdPH5, a vacuolar proton-pumping P-ATPase, was associated with both pre- and post-harvest malate contents. Moreover, MdPH5 is localized at the tonoplast and regulates malate accumulation and vacuolar pH. In addition, MdMYB73, an upstream MYB transcription factor of MdPH5, directly binds to its promoter, thereby transcriptionally activating its expression and enhancing its activity. In this way, MdMYB73 can also affect malate accumulation and vacuolar pH. Overall, this study clarifies how MdMYB73 and MdPH5 act to regulate vacuolar malate transport systems, thereby affecting malate accumulation and vacuolar pH.

作为水果中的主要有机酸,苹果酸在细胞质中产生,然后被运输到液泡中。它通过液泡质子泵、转运体和通道积聚,影响水果的口感和风味。在三种质子泵(V-ATP 酶、V-PP 酶和 P-ATP 酶)中,P-ATP 酶在将苹果酸盐转运到液泡中起着重要作用。本研究分析了开花后和贮藏期间不同阶段收集的转录组数据,结果表明液泡质子泵 P-ATP 酶 MdPH5 的表达与收获前后苹果酸含量有关。此外,MdPH5 定位于营养体,调节苹果酸的积累和液泡 pH 值。此外,MdPH5 的上游 MYB 转录因子 MdMYB73 可直接与其启动子结合,从而转录激活其表达并增强其活性。这样,MdMYB73 也能影响苹果酸的积累和液泡 pH 值。总之,本研究阐明了 MdMYB73 和 MdPH5 如何调控液泡苹果酸盐转运系统,从而影响苹果酸盐积累和液泡 pH 值。
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引用次数: 0
DNA methylation-dependent epigenetic regulation of Verticillium dahliae virulence in plants 植物黄萎病毒力的DNA甲基化表观遗传调控。
IF 3.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-09-20 DOI: 10.1007/s42994-023-00117-5
Yun-Ya Chen, Chen Zhu, Jian-Hua Zhao, Ting Liu, Feng Gao, Ying-Chao Zhang, Cheng-Guo Duan

As a conserved epigenetic mark, DNA cytosine methylation, at the 5’ position (5-mC), plays important roles in multiple biological processes, including plant immunity. However, the involvement of DNA methylation in the determinants of virulence of phytopathogenic fungi remains elusive. In this study, we profiled the DNA methylation patterns of the phytopathogenic fungus Verticillium dahliae, one of the major causal pathogens of Verticillium wilt disease that causes great losses in many crops, and explored its contribution in fungal pathogenicity. We reveal that DNA methylation modification is present in V. dahliae and is required for its full virulence in host plants. The major enzymes responsible for the establishment of DNA methylation in V. dahliae were identified. We provided evidence that DNA methyltransferase-mediated establishment of DNA methylation pattern positively regulates fungal virulence, mainly through repressing a conserved protein kinase VdRim15-mediated Ca2+ signaling and ROS production, which is essential for the penetration activity of V. dahliae. In addition, we further demonstrated that histone H3 lysine 9 trimethylation (H3K9me3), another heterochromatin marker that is closely associated with 5-mC in eukaryotes, also participates in the regulation of V. dahliae pathogenicity, through a similar mechanism. More importantly, DNA methyltransferase genes VdRid, VdDnmt5, as well as H3K9me3 methyltransferase genes, were greatly induced during the early infection phase, implying that a dynamic regulation of 5-mC and H3K9me3 homeostasis is required for an efficient infection. Collectively, our findings uncover an epigenetic mechanism in the regulation of phytopathogenic fungal virulence.

DNA胞嘧啶甲基化作为一种保守的表观遗传标记,在5'位置(5- mc)起着重要的作用,在包括植物免疫在内的多个生物过程中发挥着重要作用。然而,DNA甲基化在植物致病真菌毒力决定因素中的作用仍然难以捉摸。在这项研究中,我们分析了植物病原真菌黄萎病(Verticillium dahliae)的DNA甲基化模式,并探讨了其在真菌致病性中的作用。黄萎病是造成许多作物损失的主要病原菌之一。我们发现DNA甲基化修饰存在于大丽花中,并且是其在宿主植物中完全毒力所必需的。鉴定了大丽花DNA甲基化的主要酶。我们提供的证据表明,DNA甲基化酶介导的DNA甲基化模式的建立正调节真菌毒力,主要通过抑制一个保守的蛋白激酶vdrim15介导的Ca2+信号和ROS的产生,这是大丽花的渗透活性所必需的。此外,我们进一步证明了真核生物中与5-mC密切相关的另一种异染色质标记H3赖氨酸9三甲基化(H3K9me3)也通过类似的机制参与了大丽花致病性的调控。更重要的是,DNA甲基转移酶基因VdRid、VdDnmt5以及H3K9me3甲基转移酶基因在感染早期被大量诱导,这意味着有效感染需要对5-mC和H3K9me3稳态进行动态调控。总的来说,我们的发现揭示了植物致病性真菌毒力调控的表观遗传机制。补充信息:在线版本包含补充资料,下载地址:10.1007/s42994-023-00117-5。
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引用次数: 0
Constitutive basis of root system architecture: uncovering a promising trait for breeding nutrient- and drought-resilient crops 根系结构的组成基础:发现培育营养和抗旱作物的前景光明的性状
IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2023-09-15 DOI: 10.1007/s42994-023-00112-w
Zhigang Liu, Tongfei Qin, Michaella Atienza, Yang Zhao, Hanh Nguyen, Huajin Sheng, Toluwase Olukayode, Hao Song, Karim Panjvani, Jurandir Magalhaes, William J. Lucas, Leon V. Kochian

Root system architecture (RSA) plays a pivotal role in efficient uptake of essential nutrients, such as phosphorous (P), nitrogen (N), and water. In soils with heterogeneous nutrient distribution, root plasticity can optimize acquisition and plant growth. Here, we present evidence that a constitutive RSA can confer benefits for sorghum grown under both sufficient and limiting growth conditions. Our studies, using P efficient SC103 and inefficient BTx635 sorghum cultivars, identified significant differences in root traits, with SC103 developing a larger root system with more and longer lateral roots, and enhanced shoot biomass, under both nutrient sufficient and deficient conditions. In addition to this constitutive attribute, under P deficiency, both cultivars exhibited an initial increase in lateral root development; however, SC103 still maintained the larger root biomass. Although N deficiency and drought stress inhibited both root and shoot growth, for both sorghum cultivars, SC103 again maintained the better performance. These findings reveal that SC103, a P efficient sorghum cultivar, also exhibited enhanced growth performance under N deficiency and drought. Our results provide evidence that this constitutive nature of RSA can provide an avenue for breeding nutrient- and drought-resilient crops.

根系结构(RSA)在有效吸收磷(P)、氮(N)和水等必需养分方面起着关键作用。在养分分布不均的土壤中,根系的可塑性可以优化养分吸收和植物生长。在此,我们提出证据表明,在充足和限制性生长条件下生长的高粱都能获得组成型 RSA 的益处。我们利用钾效率高的 SC103 和钾效率低的 BTx635 两种高粱栽培品种进行了研究,发现它们的根系性状存在显著差异,其中 SC103 在养分充足和养分不足的条件下都能形成较大的根系,具有更多更长的侧根,并能提高枝条生物量。除这一构成特性外,在缺钾时,两个栽培品种的侧根发育最初都有所增加;但 SC103 仍保持了较大的根生物量。虽然缺氮和干旱胁迫抑制了高粱根和芽的生长,但 SC103 仍保持了较好的表现。这些研究结果表明,SC103 作为一种高效钾高粱栽培品种,在缺氮和干旱胁迫下也表现出更强的生长性能。我们的研究结果证明,RSA 的这种组成型特性可为培育营养和抗旱作物提供一条途径。
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引用次数: 0
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