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Detection of Ochratoxin A Mycotoxin with Graphene Nanosheets Functionalized with Selective Peptides Using Molecular Dynamics 利用分子动力学方法,用选择性肽功能化的石墨烯纳米片检测赭曲霉毒素 A霉菌毒素
IF 2.6 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-03-28 DOI: 10.1007/s13369-024-08910-0
Faisal K. Algethami, Mohamed Mastouri, Amal Rabti, Sami Ben Aoun, Saoussen El Ghoul, Amor Mosbah, Mohamed R. Elamin, Noureddine Raouafi

Ochratoxin A (OTA) is a mycotoxin commonly found in improperly stored grains, posing significant risks to food safety. Accurate detection of this mycotoxin in food matrices is crucial for ensuring consumer protection. In this study, we used in silico methods to design new peptides with high affinity and selectivity for recognizing OTA, starting crystal structures of OTA-protein complexes, which served to identify short key peptides within the interaction regions. Through deliberate point mutations, the stability and affinity of these peptides towards OTA were enhanced. To assess their suitability as capture probes for OTA sensing, we investigated the interaction of the selected peptides with OTA in a saline aqueous solution to mimic wet-lab experiments. Molecular docking, molecular dynamics simulations were used to evaluate the affinity and stability of the peptide-OTA complexes. Various parameters, including conformational changes, Molecular Mechanics Poisson–Boltzmann Surface Area energetics, the number of contacts, and the number of hydrogen bonds were analyzed to identify the most promising candidates. Among them, the peptide CWC11 has the highest affinity to OTA. Next, CWC11 peptide was used to functionalize the surfaces of two different carbonaceous nanomaterials: reduced graphene oxide (rGO) and graphene oxide (GO). The performance of the peptide-functionalized surfaces in presence of OTA was evaluated, revealing that GO exhibited superior performance compared to rGO. This study provides valuable insights into the design OTA-binding peptides and their use for the functionalization of carbonaceous surfaces, emphasizing the importance of surface selection to tune the efficiency and sensitivity of the detection.

赭曲霉毒素 A(OTA)是一种霉菌毒素,通常存在于储存不当的谷物中,对食品安全构成重大风险。准确检测食品基质中的这种霉菌毒素对于确保消费者的安全至关重要。在这项研究中,我们从 OTA 蛋白复合物的晶体结构入手,利用硅学方法设计出了具有高亲和力和高选择性的新肽,用于识别 OTA。通过有意的点突变,这些肽对 OTA 的稳定性和亲和力得到了增强。为了评估这些肽作为 OTA 感测捕获探针的适用性,我们研究了所选肽与 OTA 在生理盐水中的相互作用,以模拟湿实验室实验。我们利用分子对接和分子动力学模拟来评估多肽-OTA 复合物的亲和力和稳定性。通过分析构象变化、分子力学泊松-波尔兹曼表面积能量、接触数量和氢键数量等各种参数,确定了最有希望的候选化合物。其中,多肽 CWC11 与 OTA 的亲和力最高。接下来,CWC11 肽被用于功能化两种不同的碳质纳米材料:还原氧化石墨烯(rGO)和氧化石墨烯(GO)的表面。评估了多肽功能化表面在 OTA 存在下的性能,结果表明 GO 的性能优于 rGO。这项研究为设计 OTA 结合肽及其在碳质表面功能化中的应用提供了宝贵的见解,强调了表面选择对于调整检测效率和灵敏度的重要性。
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引用次数: 0
Decentralised Coordination in Swarm Robots Through XGBoost-Enhanced Colour Light Communication 通过 XGBoost 增强型色光通信实现蜂群机器人的分散式协调
IF 2.6 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-03-27 DOI: 10.1007/s13369-024-08923-9
Abhishek Kaushal, Anuj Kumar Sharma, Krishna Gupta

Inspired by natural swarm systems, robotic swarms aim to solve complicated problems through the emergent behaviour of coordinating robots (agents). Communication among the robots is of paramount importance for their effective coordination, cooperation, and overall performance. This research presents a colour light-based communication system for miniature mobile swarm robots, on which a pre-trained supervised machine learning model runs and is responsible for effective colour recognition, enhancing inter-robot local communication. The performance of various supervised machine learning techniques was examined, and XGBoost performed best overall, with a classification accuracy of 96.66%, an execution time of 0.403 ms, an average sensing distance of 87.38 cm, and an acceptable size of 402.1 kilobytes while running on a 32-bit embedded microcontroller. The current work also demonstrates various swarming behaviours, utilising the developed communication as proof of concept.

受自然群系统的启发,机器人群旨在通过协调机器人(代理)的突发行为来解决复杂问题。机器人之间的通信对它们的有效协调、合作和整体性能至关重要。本研究为微型移动机器人群提出了一种基于色光的通信系统,在该系统上运行一个预先训练好的监督机器学习模型,负责有效识别颜色,加强机器人之间的本地通信。对各种有监督机器学习技术的性能进行了检验,XGBoost 的总体性能最佳,分类准确率为 96.66%,执行时间为 0.403 毫秒,平均感应距离为 87.38 厘米,在 32 位嵌入式微控制器上运行时的可接受大小为 402.1 千字节。目前的工作还利用开发的通信作为概念验证,演示了各种蜂群行为。
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引用次数: 0
Influence of Enzyme Induced Carbonate Precipitation (EICP) on the Engineering Characteristics of Expansive soil 酶诱导碳酸盐沉淀(EICP)对膨胀土工程特性的影响
IF 2.6 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-03-27 DOI: 10.1007/s13369-024-08896-9
Mudassir Mehmood, Yuancheng Guo, Lei Wang, Yunlong Liu, Bantayehu Uba Uge, Sharafat Ali

Enzyme induced carbonate precipitation (EICP) is a new bio-cementation technique that utilizes plant-sourced urease to catalyze urea degradation and reaction with calcium iron, resulting in the formation of calcium carbonate (CaCO3) for soil improvement. EICP has considerable promise for novel and sustainable engineering applications such as soil strengthening, pollutant remediation, and other in situ field applications. In this study, the effect of EICP on the geotechnical characteristics of expansive soil is examined. A series of laboratory tests have been performed with an optimal concentration ratio of 0.75 mol/L. The outcomes of the compaction experiment indicated a slight increment in the dry density of the expansive soil from 15.78 to 16.71 kN/m3.Further, it diminished the optimal moisture content of the soil, decreasing it from 22.3 to 18.5%. The utilization of EICP improves the soil mechanical characteristics, reducing swelling pressure by 80% and increasing the UCS, cohesion, friction angle, unsoaked and soaked CBR by 66%, 44%, 49%, 441%, and 430%, approximately. Additionally, it leads to a significant decrease in soil permeability, approximately 63%. Moreover, SEM and XRD analysis confirmed the presence of CaCO3 content in the treated soil. The experimental findings indicated that the EICP method holds promise in enhancing expansive soil within engineering projects.

摘要 酶诱导碳酸盐沉淀(EICP)是一种新的生物固化技术,它利用植物源脲酶催化尿素降解并与钙铁反应,从而形成碳酸钙(CaCO3)用于土壤改良。EICP 在土壤加固、污染物修复和其他原位现场应用等新型和可持续工程应用方面前景广阔。本研究探讨了 EICP 对膨胀性土壤岩土特性的影响。以 0.75 mol/L 的最佳浓度比进行了一系列实验室试验。压实实验结果表明,膨胀土的干密度从 15.78 kN/m3 增加到 16.71 kN/m3。EICP 的使用改善了土壤的机械特性,使膨胀压力降低了 80%,UCS、内聚力、摩擦角、未浸泡 CBR 和浸泡 CBR 分别提高了 66%、44%、49%、441% 和 430%。此外,它还导致土壤渗透性显著降低,约为 63%。此外,SEM 和 XRD 分析证实了处理过的土壤中含有 CaCO3。实验结果表明,EICP 方法有望改善工程项目中的膨胀性土壤。
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引用次数: 0
Residual Convolutional Neural Network-Based Dysarthric Speech Recognition 基于残差卷积神经网络的聋哑语音识别
IF 2.6 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-03-27 DOI: 10.1007/s13369-024-08919-5
Raj Kumar, Manoj Tripathy, R. S. Anand, Niraj Kumar

People with dysarthric speech face problems communicating with others and voice-based smart devices. This paper presents the development of a spatial residual convolutional neural network (RCNN)-based dysarthric speech recognition (DSR) system to improve communication for individuals with dysarthric speech. The RCNN model is simplified to an optimal number of layers. The system utilizes a speaker-adaptive approach, incorporating transfer learning to leverage knowledge learned from healthy individuals and a new data augmentation technique to address voice hoarseness in patients. The dysarthric speech is preprocessed using a novel voice cropping technique based on erosion and dilation methods to eliminate unnecessary pauses and hiccups in the time domain. The isolated word recognition accuracy improved by nearly 8.16% for patients with very low intelligibility and 4.74% for patients with low intelligibility speech compared to previously reported results. The proposed DSR system gives the lowest word error rate of 24.09% on the UASpeech dysarthric speech datasets of 15 dysarthric speakers.

有语言障碍的人面临着与他人和基于语音的智能设备交流的问题。本文介绍了基于空间残差卷积神经网络(RCNN)的发音障碍语音识别(DSR)系统的开发情况,以改善发音障碍者的交流。RCNN 模型被简化为最佳层数。该系统采用说话者自适应方法,结合迁移学习来利用从健康人身上学到的知识,并采用新的数据增强技术来解决患者声音嘶哑的问题。使用基于侵蚀和扩张方法的新型语音裁剪技术对听力障碍语音进行预处理,以消除时域中不必要的停顿和打嗝。与之前报告的结果相比,极低智能度患者的孤立词识别准确率提高了近 8.16%,低智能度语音患者的孤立词识别准确率提高了 4.74%。在由 15 位肢体畸形者组成的 UASpeech 言语障碍数据集上,所提出的 DSR 系统的单词错误率最低,仅为 24.09%。
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引用次数: 0
Multi-objective Optimization Framework for Trade-Off Among Pedestrian Delays and Vehicular Emissions at Signal-Controlled Intersections 信号控制交叉口行人延迟与车辆排放权衡的多目标优化框架
IF 2.6 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-03-27 DOI: 10.1007/s13369-024-08898-7
Görkem Akyol, Sadullah Göncü, Mehmet Ali Silgu

Traffic congestion has several adverse effects on urban traffic networks. Increased travel times of vehicles, with the addition of excessive greenhouse emissions, can be listed as harmful effects. To address these issues, transportation engineers aim to reduce private car usage, reduce travel times through different control strategies, and mitigate harmful effects on urban networks. In this study, we introduce an innovative approach to optimizing traffic signal control settings. This methodology takes into account both pedestrian delays and vehicular emissions. Non-dominated sorting genetic algorithm-II and Multi-objective Artificial Bee Colony algorithms are adopted to solve the multi-objective optimization problem. The vehicular emissions are modeled through the MOVES3 emission model and integrated into the utilized microsimulation environment. Initially, the proposed framework is tested on a hypothetical test network, followed by a real-world case study. Results indicate a significant improvement in pedestrian delays and lower emissions.

交通拥堵会对城市交通网络产生多种不利影响。车辆行驶时间的增加以及温室气体的过度排放都可列为有害影响。为了解决这些问题,交通工程师们希望减少私家车的使用,通过不同的控制策略缩短出行时间,减轻对城市交通网络的有害影响。在本研究中,我们介绍了一种优化交通信号控制设置的创新方法。该方法同时考虑了行人延迟和车辆排放。采用非支配排序遗传算法-II 和多目标人工蜂群算法来解决多目标优化问题。车辆排放通过 MOVES3 排放模型建模,并集成到所使用的微观模拟环境中。首先,在一个假设的测试网络上对所提出的框架进行了测试,然后进行了实际案例研究。结果表明,行人延迟得到明显改善,排放也有所降低。
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引用次数: 0
Design and Performance Evaluation of a Mid-Range Airborne Wind Turbine 中程机载风力涡轮机的设计与性能评估
IF 2.6 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-03-27 DOI: 10.1007/s13369-024-08926-6
Morteza Bayati

The focus of this study is on the aerodynamic design of a 2000-W airborne wind turbine (AWT) situated 100 m above ground level. AWTs harness higher wind speeds at higher altitudes, making them more efficient than ground-based turbines in generating power. To achieve the desired performance, numerical methods, such as blade element momentum (BEM) and computational fluid dynamics (CFD) were employed for the aerodynamic design and analysis of the AWT system. The rotor cross-section of the three-blade rotor incorporates S223 and S822 airfoils, which are derived from the conventional NREL airfoils. The diffuser section of the AWT has an elliptical curve to accommodate the maximum volume of helium gas. Through simulations using both CFD and BEM, the performance of the bare rotor was assessed, as well as the diffuser-augmented wind turbine (DAWT) under design and off-design conditions. The results demonstrated that the DAWT is capable of meeting the desired power output in all scenarios. Moreover, the findings indicated that using the diffuser enhances the rotor output power coefficient by 18% and prevents significant power loss during off-design conditions. This highlights the effectiveness of the rotor-diffuser assembly in maximizing power generation and ensuring consistent performance of the AWT system.

本研究的重点是位于离地面 100 米高空的 2000 瓦机载风力涡轮机(AWT)的空气动力学设计。机载风力涡轮机能在更高的高度上利用更高的风速,使其发电效率高于地面涡轮机。为实现预期性能,在 AWT 系统的空气动力学设计和分析中采用了叶片元素动量(BEM)和计算流体动力学(CFD)等数值方法。三叶片转子的横截面采用了 S223 和 S822 翼面,这两种翼面源自 NREL 的传统翼面。AWT 的扩散器部分采用椭圆曲线,以容纳最大体积的氦气。通过使用 CFD 和 BEM 进行模拟,评估了裸转子以及扩散器增强型风力涡轮机 (DAWT) 在设计和非设计条件下的性能。结果表明,DAWT 在所有情况下都能达到预期的功率输出。此外,研究结果表明,使用扩散器可将转子输出功率系数提高 18%,并可防止非设计条件下的显著功率损失。这凸显了转子-扩散器组件在最大限度提高发电量和确保 AWT 系统性能一致性方面的有效性。
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引用次数: 0
Immersion and Invariance-Based Nonlinear Control Synthesis for Depth Position of an AUV: Tracking and Regulation 基于浸入和不变性的 AUV 深度定位非线性控制合成:跟踪和调节
IF 2.6 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-03-27 DOI: 10.1007/s13369-024-08915-9
Ravishankar P. Desai, Narayan S. Manjarekar

This paper addresses the tracking control problem associated with the diving motion system of a torpedo-like shape autonomous underwater vehicle (AUV). A decoupled and reduced-order three degrees-of-freedom (3-DOF) nonlinear model is employed to represent the dynamics of the diving motion system for depth position control. The control objective is to track the demanded depth position in the presence of uncertainties and disturbances. A control law based on the immersion and invariance (I &I) technique is synthesized to achieve the control objectives. The proposed control law effectively tracks a stable, lower-order target dynamic system immersed within a three-dimensional manifold. Additionally, the regulation problem is treated as a specialized case of tracking, with a known depth serving as the reference input to be regulated. The performance of the proposed control law is assessed through simulation studies that consider various scenarios. The simulation study evaluates the robustness of the proposed control law resilience against modelling uncertainties and underwater disturbances. The simulations utilize the MAYA AUV, incorporating experimentally validated diving motion parameters. The proposed control law’s quantitative analysis and computational performance show better performance against the benchmark controller.

本文探讨了与鱼雷状自主潜水器(AUV)下潜运动系统相关的跟踪控制问题。采用一个解耦和降阶三自由度(3-DOF)非线性模型来表示深度位置控制的潜水运动系统动力学。控制目标是在存在不确定性和干扰的情况下跟踪所要求的深度位置。为实现控制目标,合成了基于浸入和不变性(I &I )技术的控制法则。所提出的控制法则能有效跟踪沉浸在三维流形中的稳定、低阶目标动态系统。此外,调节问题被视为跟踪的一种特殊情况,以已知深度作为需要调节的参考输入。通过考虑各种情况的仿真研究,对所提出的控制法则的性能进行了评估。模拟研究评估了所提出的控制法则对建模不确定性和水下干扰的鲁棒性。模拟使用了 MAYA AUV,其中包含了经过实验验证的潜水运动参数。与基准控制器相比,所提出的控制法则的定量分析和计算性能显示出更好的性能。
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引用次数: 0
Production of NiCoMo-Supported Ni Foam for Electrocatalytic Oxidation of Methanol: Experimental and RSM Analysis 生产用于甲醇电催化氧化的镍钴钼支撑镍泡沫:实验和 RSM 分析
IF 2.6 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-03-26 DOI: 10.1007/s13369-024-08905-x
Başak Doğru Mert, Beyza Nur Demir, Cansu Edis, Şenay Akyıldız, Ceyla Özgür, Mehmet Erman Mert

The Ni-, Co-, and Mo-supported Ni foam (NiF–NiCoMo) was produced via galvanostatic method, and electrooxidation of methanol in alkaline medium was examined. The characterization was achieved using field emission scanning electron microscopy, energy-dispersive X-ray, and X-ray diffraction analysis. The electrochemical behavior was determined via cyclic voltammetry and chronoamperometry analysis. The contribution of each transition metal to electrocatalytic performance of NiF was monitored via mono, binary, and ternary modifications of each transition metal (Ni, Co, and Mo) for several amounts (5, 10, and 15 μg). Experiments were performed to determine the influence of catalyst amounts, methanol concentration, and scan rate parameters. The impacts of independent parameters on methanol electrooxidation were statistically investigated using Design-Expert software. The ability to analyze multiple parameters with a limited number of experimental performances is one of the method’s key benefits. The developed model showed that 9.41 and 14.03 µg catalyst amounts were the appropriate values for NiF–NiMo and NiF–NiCoMo achieving optimal circumstances, respectively.

通过静电法制备了镍、钴和钼支撑的泡沫镍(NiF-NiCoMo),并考察了其在碱性介质中对甲醇的电氧化作用。利用场发射扫描电子显微镜、能量色散 X 射线和 X 射线衍射分析实现了表征。电化学行为是通过循环伏安法和计时器分析法确定的。通过对每种过渡金属(镍、钴和钼)进行一元、二元和三元修饰(5、10 和 15 微克),监测了每种过渡金属对 NiF 电催化性能的贡献。实验确定了催化剂用量、甲醇浓度和扫描速率参数的影响。使用 Design-Expert 软件对独立参数对甲醇电氧化的影响进行了统计分析。该方法的主要优点之一是能够利用有限的实验性能分析多个参数。所建立的模型显示,9.41 和 14.03 µg 催化剂用量分别是 NiF-NiMo 和 NiF-NiCoMo 达到最佳情况的合适值。
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引用次数: 0
Investigation on Dimensional Scaling and Anchor Points Analysis of the Planar Membrane Reflector 平面膜反射器的尺寸缩放和锚点分析研究
IF 2.6 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-03-26 DOI: 10.1007/s13369-024-08917-7
Swapnil D. Shinde, S. H. Upadhyay

For the satellite antenna, a large aperture reflector is a contemporary need for space agencies worldwide. The space-borne membrane structures are the promising solution for the compact and lightweight reflector antenna. The objective of the study is to investigate the effect of dimension scaling on tension force with different anchor point configurations of the rectangular membrane reflector. The study also aims towards the development of the generalized tension force relation for the scaled dimensions of planar membrane reflectors. The numerical and experimental investigation for loading analysis of membrane reflector with different scale sizes and anchor point configurations is carried out. The study presents the novel equation for average diagonal stresses with different anchor points configurations of membrane reflector. The numerical results of loading analysis are found consistent with the experimental outcomes. Based on the results, the novel trend-line equation between the tension force and the scaling factor is developed. From the developed trend-line equation, the tension force can be directly calculated for any size of a reflector. The relation between the number of anchor points and the size of the reflector is discussed. The findings of this research provide insights to a novel relation for average diagonal stresses and tension force trend-line equations that can be implemented to any scaled sizes of the rectangular membrane reflector.

对于卫星天线而言,大孔径反射器是全球航天机构的当代需求。空间膜结构是紧凑型轻质反射天线的理想解决方案。本研究的目的是调查矩形膜反射器不同锚点配置下尺寸缩放对拉力的影响。研究还旨在为平面膜反射器的缩放尺寸建立广义的拉力关系。针对不同尺度尺寸和锚点配置的膜反射器的加载分析进行了数值和实验研究。研究提出了膜反射器不同锚点配置下平均对角线应力的新方程。加载分析的数值结果与实验结果一致。在此基础上,建立了拉力与缩放因子之间的新型趋势线方程。根据所建立的趋势线方程,可以直接计算出任何尺寸反射器的拉力。研究还讨论了锚点数量与反射器尺寸之间的关系。这项研究的结果为平均对角线应力和拉力趋势线方程提供了一种新的关系,这种关系可适用于任何尺寸的矩形膜反射器。
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引用次数: 0
Investigation on Effect of Fillets on the Characteristics of Relay Electrical Contacts 调查锉刀对继电器电气触点特性的影响
IF 2.6 4区 综合性期刊 Q2 MULTIDISCIPLINARY SCIENCES Pub Date : 2024-03-26 DOI: 10.1007/s13369-024-08913-x
Thanigaivelraja Mohandoss, Femi Robert

This article investigates the effect of various fillets on the performance characteristics of relay electrical contacts. Finite element modeling (FEM) of a relay electrical contact is designed in 3D using the COMSOL Multiphysics simulation tool. Copper material is assigned to the electrical contact pairs. Analysis is carried out for the heights of 0.5 mm and 1 mm. Also, the structure of the contact surface is fine-tuned by using different fillets of 1.5 mm, 1 mm, and 0.5 mm. The voltages of 1 V–10 V are offered, and their performance is evaluated. The key parameters to design the contacts are identified. The electrical, thermal, and mechanical analysis is carried out. The parameters such as current density, contact interface temperature, stress, and pressure are simulated using FEM. The results present the evaluation of relays with different heights through different fillets in various voltage ranges. The results demonstrate that the low fillet radii have a higher contact area and exhibit low current density, low contact pressure, and low stress, which is more reliable and efficient. This study will be highly beneficial for relay and circuit breakers manufacturers.

本文研究了各种圆角对继电器电气触点性能特征的影响。使用 COMSOL Multiphysics 仿真工具对继电器电触点进行三维有限元建模设计。电触点对采用铜材料。对 0.5 毫米和 1 毫米的高度进行了分析。此外,还通过使用 1.5 毫米、1 毫米和 0.5 毫米的不同圆角对接触面的结构进行了微调。电压范围为 1 V-10 V,并对其性能进行了评估。确定了设计触点的关键参数。进行了电气、热和机械分析。使用有限元模拟了电流密度、触点界面温度、应力和压力等参数。结果显示了在不同电压范围内通过不同圆角对不同高度继电器的评估。结果表明,圆角半径越小,接触面积越大,电流密度越低,接触压力越小,应力也越小,因而更可靠、更高效。这项研究将对继电器和断路器制造商大有裨益。
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引用次数: 0
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Arabian Journal for Science and Engineering
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