Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology最新文献_第2页

Effects of dietary N-carbamylglutamate (NCG) supplementation on arginine biosynthesis, metabolism, digestion and mTOR signaling pathway gene expression in Eriocheir sinensis 饲粮中添加n -氨甲酰谷氨酸（NCG）对中华绒螯蟹精氨酸生物合成、代谢、消化及mTOR信号通路基因表达的影响

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-11-17 DOI: 10.1016/j.cbpb.2025.111178

Zhiqiang Liu , Jianyang Sun , Aoya Shi , Chengyi Weng , Anjun Xiang , Mengna Hou , Mengjie Zhao , Chen Zheng , Yongxu Cheng , Xiaozhen Yang

N-carbamylglutamate (NCG) is an activator of arginine biosynthesis, but its specific role in crustaceans remains poorly understood. This study aimed to investigate the effects of NCG on arginine biosynthesis capacity, metabolism, digestion, and the gene expression of the mTOR signaling pathway in Eriocheir sinensis. In Experiment 1, hepatopancreas was cultured in vitro with NCG medium (0, 65, 75, and 85 mg/L NCG). In Experiment 2, crabs were fed either regular feed or NCG feed (content: 302.96 ± 4.07 mg/kg) for 14 days. In Experiment 1, NCG significantly upregulated pyrroline-5-carboxylate synthase (p5cs) gene expression (P < 0.05), an enzyme that is related to arginine biosynthesis. Similarly, dietary NCG upregulated p5cs expression and significantly increased the activities of carbamoyl-phosphate synthase-1 (CPS-1) and P5CS in the hepatopancreas and intestine (P < 0.05). Metabolomics analysis indicated that NCG altered the metabolic profile of the hepatopancreas, promoting cholesterol metabolism, and arginine and proline metabolism. In the intestine, trypsin and α-amylase activities were significantly elevated (P < 0.05). NCG also altered the composition of intestinal microflora, with an increase in Proteobacteria and in the ratio of Firmicutes to Bacteroidota. Additionally, NCG increased the content of signaling molecule nitric oxide (NO) and upregulated the expression of genes in the mTOR signaling pathway (P < 0.05). In conclusion, NCG supplementation enhanced arginine biosynthesis capacity, stimulated intestinal enzymatic activities, and upregulated mTOR signaling pathway gene expression in Eriocheir sinensis, indicating the potential for improved metabolism and digestion.

n -氨甲酰谷氨酸（NCG）是精氨酸生物合成的激活剂，但其在甲壳类动物中的具体作用尚不清楚。本研究旨在探讨NCG对中华绒螯蟹精氨酸生物合成能力、代谢、消化及mTOR信号通路基因表达的影响。实验1采用NCG培养基（0、65、75、85 mg/L NCG）体外培养肝胰腺。试验2分别饲喂常规饲料和NCG饲料（含量：302.96 ± 4.07 mg/kg），饲喂14 d。实验1中，NCG显著上调吡咯-5-羧酸合成酶（p5cs）基因表达

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引用次数: 0

Corrigendum to “Transfection with SV40 LT promotes oxidative damage in primary cultures of California sea lion muscle cells” [Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Volume 280 (2025) 1–9/111138]. “转染SV40 LT促进加州海狮肌肉细胞原代培养的氧化损伤”的更正[比较生物化学和生理学B部分：生物化学和分子生物学卷280(2025)1-9/111138]。

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-11-13 DOI: 10.1016/j.cbpb.2025.111175

Andrea C. Murillo-Cisneros , Ramón Gaxiola-Robles , Claudia J. Hernández-Camacho , Martha Reyes-Becerril , Orlando Lugo-Lugo , Tania Zenteno-Savín

引用次数: 0

Temporal dynamics of hepatic mRNA levels of carbohydrate and lipid metabolism-related genes in rainbow trout: Impact of a plant-based diet from first feeding 虹鳟鱼肝脏碳水化合物和脂质代谢相关基因mRNA水平的时间动态：首次饲喂植物性饮食的影响

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-11-12 DOI: 10.1016/j.cbpb.2025.111176

Cristina Fernández-Maestú , Maud Martinat , Jessica Calo , Cristina Velasco , José L. Soengas , Jérôme Roy , Ayelén M. Blanco

Early-life nutrition plays a critical role in shaping metabolic trajectories in fish, particularly in carnivorous species like rainbow trout (Oncorhynchus mykiss), which displays limited capacity to utilize plant-based diets. This study investigated the temporal changes in mRNA abundance of key hepatic genes involved in carbohydrate and lipid metabolism from first feeding to 100 days post-first meal in rainbow trout fry fed either a commercial-like or fully plant-based diet. mRNA abundance was quantified by RT-qPCR at six time points, revealing marked ontogenetic shifts in metabolic gene profiles regardless of diet. Except for gck (encoding glucokinase), glycolytic and gluconeogenic enzymes generally showed declining expression over time, while genes involved in lipogenesis, notably fasn (encoding fatty acid synthase) and acly (encoding ATP citrate synthase), increased progressively—suggesting a developmental shift toward lipogenesis and energy storage pathways. Despite similar patterns observed under both dietary conditions, fish fed the plant-based diet exhibited attenuated temporal transcriptional changes in some genes. These patterns were consistent with previously reported growth deficits in the same fish, potentially reflecting impaired metabolic acclimation. Our findings highlight that while the liver undergoes dynamic metabolic maturation during early development, plant-based diets may hinder this process, particularly in lipid handling. This work provides a molecular basis for improving feed formulations and supports the need for strategies that enhance metabolic flexibility during critical early stages in aquaculture species.

生命早期的营养在塑造鱼类的代谢轨迹中起着至关重要的作用，特别是在虹鳟鱼（Oncorhynchus mykiss）等肉食性物种中，它们利用植物性饮食的能力有限。本研究研究了虹鳟鱼鱼苗在第一次喂食到第一次用餐后100 天，与碳水化合物和脂质代谢有关的关键肝脏基因mRNA丰度的时间变化，这些基因分别饲喂商业化或完全植物性饮食。通过RT-qPCR在六个时间点量化mRNA丰度，揭示了代谢基因谱在个体发生方面的显著变化，而与饮食无关。除了gck（编码葡萄糖激酶）外，糖酵解酶和糖异生酶的表达随着时间的推移普遍下降，而参与脂肪生成的基因，尤其是fasn（编码脂肪酸合成酶）和acly（编码ATP柠檬酸合成酶）的表达则逐渐增加，这表明脂肪生成和能量储存途径的发育转变。尽管在两种饮食条件下观察到相似的模式，但喂食植物性饮食的鱼在某些基因中表现出减弱的时间转录变化。这些模式与先前报道的同一种鱼的生长缺陷一致，可能反映了代谢适应受损。我们的研究结果强调，虽然肝脏在早期发育过程中经历了动态代谢成熟，但植物性饮食可能会阻碍这一过程，特别是在脂质处理方面。这项工作为改进饲料配方提供了分子基础，并支持在关键的早期阶段提高水产养殖物种代谢灵活性的策略的需求。

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引用次数: 0

The nacre protein Hcperlucin provides antibacterial defense following Aeromonas hydrophila infection and crystal morphological modification during shell biomineralization in Hyriopsis cumingii 珠光蛋白高lucin在三角帆蚌壳生物矿化过程中对嗜水气单胞菌感染和晶体形态改变具有抗菌防御作用。

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-11-02 DOI: 10.1016/j.cbpb.2025.111172

Xiaoyang Tan , Jiayi Zhang , Haohan Xu , Kang Cheng , Yihang Zhang , Jingwen Pu , Can Jin , Wen Luo

C-type lectins trigger innate immunity by recognizing conserved bacteria features and also play a functional role in shell formation. However, most studies focus exclusively on one of these functions. Hcperlucin, a typical C-type lectin, is recognized as a matrix protein in the nacreous layer of the shell of Hyriopsis cumingii. In this study, the expression level of Hcperlucin in the mantle and hemocytes was significantly upregulated following Aeromonas hydrophila stimulation. Inhibition of Hcperlucin led to a downregulation of the expression of theromacin and big defensin. Recombinant protein SUMO-Hcperlucin demonstrated a significant inhibitory effect on bacterial growth. These results suggest that Hcperlucin plays a crucial role in innate immunity. Furthermore, the suppression of Hcperlucin at both the transcriptional and protein levels resulted in the disappearance of nacre platelets from the nucleus. SUMO-Hcperlucin promoted the formation of spherical crystal structures. These results indicate that Hcperlucin is directly involved in the morphological modification of crystals during shell biomineralization. Additionally, the relatively high expression of Hcperlucin in hemocytes within the pearl sac, suggests that Hcperlucin also participates in the initial stages of pearl biomineralization. Overall, these results deepen our understanding of the role of immune components in biomineralization.

c型凝集素通过识别保守的细菌特征触发先天免疫，并在外壳形成中发挥功能作用。然而，大多数研究只关注其中一种功能。高lucin是一种典型的c型凝集素，被认为是三角帆蚌（Hyriopsis cumingii）壳珍珠层的基质蛋白。在本研究中，受嗜水气单胞菌刺激后，套膜和血细胞中高lucin的表达水平显著上调。抑制高lucin导致theromacin和大防御素的表达下调。重组蛋白sumo - hperlucin对细菌生长有明显的抑制作用。这些结果表明，hperlucin在先天免疫中起着至关重要的作用。此外，在转录和蛋白水平上抑制高lucin导致珍珠层血小板从细胞核中消失。sumo -超lucin促进了球形晶体结构的形成。这些结果表明，高lucin直接参与了壳生物矿化过程中晶体的形态改变。此外，在珍珠囊内的血细胞中，hperlucin的相对高表达表明hperlucin也参与了珍珠生物矿化的初始阶段。总的来说，这些结果加深了我们对免疫成分在生物矿化中的作用的理解。

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引用次数: 0

N-terminal domains and site-specific glycosylation regulate the secretion of avian melanocortin inverse agonists, agouti signaling protein (ASIP) and agouti-related protein (AGRP) n端结构域和位点特异性糖基化调节鸟类黑素皮质素逆激动剂、针刺信号蛋白（ASIP）和针刺相关蛋白（AGRP）的分泌。

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-11-01 DOI: 10.1016/j.cbpb.2025.111174

Hibiki Fukuchi , Ryoya Watanabe , Yuna Iida , Saya Nakano , Aya Mizutani , Tatsuhiko Abo , Sayaka Aizawa , Sakae Takeuchi

Agouti signaling protein (ASIP) and agouti-related protein (AGRP) are paralogous inverse agonists of melanocortin receptors with distinct physiological roles, but their structural and biochemical properties in birds remain poorly understood. Here, we characterized chicken ASIP and AGRP proteins. Analysis of available sequences revealed that a motif resembling the mammalian proprotein convertase 1/3 (PC1/3, also known as PCSK1) cleavage site is conserved across a broad range of avian orders, but Western blot analysis of transfected Chinese hamster ovary (CHO-K1) cells and chicken hypothalamus detected no cleavage, suggesting that avian AGRP may not be post-translationally processed at this site. Chicken ASIP mRNA contains an in-frame upstream ATG (uATG) and a putative N-linked glycosylation site at Asn-42, both conserved across multiple avian orders. Overexpression in CHO-K1 cells showed that ASIP translated from either ATG produces a mature protein of the same size that is N-glycosylated at Asn-42 and exhibits markedly lower secretion efficiency than AGRP. Domain-swapping experiments revealed that the N-terminal domain reduces secretion, whereas a naturally occurring ASIP-b variant with an additional N-glycan at Asn-47 shows enhanced secretion. Proteasome inhibition increased intracellular ASIP, and endoglycosidase H (Endo H) sensitivity indicated endoplasmic reticulum (ER) retention, suggesting that the N-terminal domain limits secretion via ER-associated proteasomal degradation. These findings reveal species-specific post-translational regulation of avian melanocortin inverse agonists, in which N-terminal features and site-specific N-glycosylation determine secretion efficiency, likely contributing to their distinct roles in pigmentation and hypothalamic energy balance.

针刺信号蛋白（ASIP）和针刺相关蛋白（AGRP）是黑素皮质素受体的同源反向激动剂，具有不同的生理作用，但其在鸟类中的结构和生化特性尚不清楚。在这里，我们对鸡的ASIP和AGRP蛋白进行了表征。对现有序列的分析显示，一个类似于哺乳动物蛋白转换酶1/3 （PC1/3，也称为PCSK1）切割位点的基序在许多鸟类目中都是保守的，但转染的中国仓鼠卵巢（CHO-K1）细胞和鸡下丘脑的Western blot分析未检测到切割，这表明鸟类AGRP可能不会在该位点进行翻译后加工。鸡ASIP mRNA包含帧内上游ATG （uATG）和Asn-42假定的n-链糖基化位点，这两个位点在多个鸟类目中都是保守的。CHO-K1细胞中的过表达表明，从任一ATG翻译的ASIP产生相同大小的成熟蛋白，该蛋白在Asn-42处被n-糖基化，其分泌效率明显低于AGRP。结构域交换实验表明，n端结构域会减少分泌，而天然存在的ASIP-b变体在Asn-47处增加了n-聚糖，则会增加分泌。蛋白酶体抑制增加了细胞内ASIP，内糖苷酶H （Endo H）敏感性表明内质网（ER）保留，表明n端结构域限制了内质网相关蛋白酶体降解的分泌。这些发现揭示了鸟类黑素皮质素逆激动剂的物种特异性翻译后调控，其中n端特征和位点特异性n糖基化决定了分泌效率，可能有助于它们在色素沉着和下丘脑能量平衡中的独特作用。

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引用次数: 0

Dietary genistein mitigated arsenic-induced growth inhibition, oxidative stress, inflammation, apoptosis, disruption of tight junction proteins, and intestinal histomorphological damage in Nile tilapia 饲料染料木素减轻砷诱导的尼罗罗非鱼生长抑制、氧化应激、炎症、细胞凋亡、紧密连接蛋白破坏和肠道组织形态学损伤。

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-10-27 DOI: 10.1016/j.cbpb.2025.111173

Badriyah S. Alotaibi , Amany Abdel-Rahman Mohamed , Saed A. Althobaiti , Khalid S. Alotaibi , Mohamed Mohamed Soliman , Mohamed M.M. Metwally , Tarek Khamis , Yasmina M. Abd-Elhakim , Nawal Alsubaie , Rowida E. Ibrahim

Arsenic is a widespread trace element that adversely affects fish health and growth. This study investigated the potential of a genistein-fortified diet (500 mg/kg) to mitigate effects of arsenic exposure in Oreochromis niloticus. The 60-d experiment consisted of four groups: control diet, genistein (GE; 500 mg/kg) diet, and these two same diets but concurrent waterborne exposure to 10 μg/L As₂O₃. Arsenic exposure induced growth inhibition with decreased growth hormone concentration, reduced digestive enzyme (trypsin, amylase, and lipase) activities, and reduced total intestinal bacterial and Lactobacillus count. A decrease in the activity of superoxide dismutase and catalase, and reduced glutathione content with increased malondialdehyde concentration were also measured in the arsenic-exposed fish. Arsenic exposure also decreased gene expression of intestinal transporters (solute carrier family (slc) 26a6, slc4a4, ATPase Na⁺/K⁺ transporting subunit alpha 3, and slc15a1a) and the tight junction protein zonula occludens-1. Furthermore, up-regulation of the inflammatory (nuclear factor kappa β65, interleukin-1β, interleukin-6, and interleukin-8) and apoptotic (caspase-3 and bcl-2 associated X-protein) gene expression markers was noted in the arsenic-exposed fish. Feeding the arsenic-exposed fish on a GE diet improved their growth and intestinal microenvironment and mitigated the changes in oxidative stress, inflammatory, and apoptotic cascades. Notably, GE supplementation could enhance the Nile tilapia growth performance during arsenic exposure possibly by improving intestinal structure and function.

砷是一种广泛存在的微量元素，对鱼类的健康和生长有不利影响。本研究探讨了染料木素强化日粮（500 mg/kg）减轻尼罗ticus中砷暴露影响的潜力。60 d的试验分为四组：对照饲料，染料木素（GE; 500 mg/kg）饲料，这两组饲料相同，但同时暴露于10 μg/L As₂O₃。砷暴露导致生长抑制，生长激素浓度降低，消化酶（胰蛋白酶、淀粉酶和脂肪酶）活性降低，肠道细菌总数和乳杆菌数量减少。在砷暴露的鱼类中，超氧化物歧化酶和过氧化氢酶的活性降低，并且随着丙二醛浓度的增加，谷胱甘肽含量降低。砷暴露还降低了肠道转运蛋白（溶质载体家族（slc） 26a6、slc4a4、atp酶Na+/K+转运亚基α 3和slc15a1a）和紧密连接蛋白闭塞带-1的基因表达。此外，在砷暴露的鱼类中，炎症（核因子kappa β65、白细胞介素-1β、白细胞介素-6和白细胞介素-8）和凋亡（caspase-3和bcl-2相关x蛋白）基因表达标记上调。用转基因饲料喂养砷暴露鱼，改善了它们的生长和肠道微环境，减轻了氧化应激、炎症和凋亡级联反应的变化。值得注意的是，添加GE可能通过改善肠道结构和功能来提高砷暴露期间尼罗罗非鱼的生长性能。

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引用次数: 0

Effect of the sdc4 gene knockdown on muscle development in zebrafish sdc4基因敲低对斑马鱼肌肉发育的影响

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-10-25 DOI: 10.1016/j.cbpb.2025.111171

Ai-Jin Wang, Chang Du, Hong Liu, Huan-Ling Wang

The growth and regeneration of skeletal muscle are closely related to syndecan-4 (Sdc4), which is a type I transmembrane heparan sulfate proteoglycan belonging to the syndecan family. However, it remains unclear how the sdc4 gene affects fish muscle development. Therefore, an sdc4 knockdown zebrafish line (sdc4^−/−) was generated by CRISPR/Cas9 technology in this study, and its phenotypes were analyzed. The results revealed that sdc4^−/− zebrafish exhibited reduced body length and weight compared to the wild-type (WT) at 90 days post fertilization (dpf). Furthemore, sdc4^−/− zebrafish also showed a significantly larger number of muscle fibers, and significantly reduced individual muscle fiber cross-sectional area. The mRNA expression levels of genes associated with myogenic regulatory factors (MRFs) and the wnt/β-catenin pathway were all significantly downregulated. Based on dual luciferase reporter assays, sdc4 gene expression was regulated by the transcription factor myocyte enhancer factor 2aa (Mef2aa) and miR-141-3p, which bind to its promoter and 3′ untranslated region (UTR), respectively. Additionally, the reduced average swimming speed and distance observed in sdc4^−/− zebrafish at 90 dpf were concomitant with a significant downregulation of mitochondrial respiratory chain complex-related genes and a reduction in ATP concentration. This research aids understanding of sdc4 function in fish and may provide a new perspective for studying the molecular mechanisms of muscle growth and development.

骨骼肌的生长再生与syndecan-4 （Sdc4）密切相关，syndecan-4是一种属于syndecan家族的I型跨膜硫酸肝素蛋白多糖。然而，目前尚不清楚sdc4基因是如何影响鱼类肌肉发育的。因此，本研究利用CRISPR/Cas9技术生成sdc4敲低斑马鱼系（sdc4−/−），并对其表型进行分析。结果表明，与野生型（WT）相比，sdc4−/−斑马鱼在受精后90天（dpf）的体长和体重都有所减少。此外，sdc4−/−斑马鱼的肌纤维数量也显著增加，个体肌纤维横截面积显著减少。与肌生成调节因子（MRFs）和wnt/β-catenin通路相关的基因mRNA表达水平均显著下调。基于双荧光素酶报告基因分析，sdc4基因的表达受到转录因子myocyte enhancer factor 2aa （Mef2aa）和miR-141-3p的调控，这两个转录因子分别与sdc4的启动子和3′非翻译区（UTR）结合。此外，在90 dpf时，sdc4 - / -斑马鱼的平均游泳速度和游泳距离的降低伴随着线粒体呼吸链复合物相关基因的显著下调和ATP浓度的降低。该研究有助于了解sdc4在鱼类中的功能，并为研究肌肉生长发育的分子机制提供了新的视角。

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引用次数: 0

Identification of effective plant-based oils for use in aquafeed: An evaluation of impact on gamete quality and developmental success using zebrafish (Danio rerio) as a screening organism 鉴定用于水产饲料的有效植物油：以斑马鱼（Danio rerio）作为筛选生物对配子质量和发育成功的影响的评估

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-10-24 DOI: 10.1016/j.cbpb.2025.111169

Seyed-Mohammadreza Samaee , Alicia Estévez , Louis R. D'Abramo

To evaluate the effectiveness of zebrafish as a screening system for identifying appropriate plant oils (POs) for aquafeed, Artemia nauplii (AN) were enriched with three single- cultivar olive oils (OO): Koroneiki, Parseh, and Arghavan. The resulting AN (ANKor, ANPar, ANArg, and AN36 [36 h starved AN, control]) were then fed to 360 fish (3.5 cm) for one month. The fatty acid (FA) profile of the AN was reflected in the ova and influenced both sperm motility and density, which in turn affected the fertilizability. The highest fertilizability and accelerated hatching were observed in the ANKor group. In this group, fish produced sperms with the highest motility and ova characterized by higher levels of 18:1n-9, 22:6n-3/20:5n-3 and lower 18:3n-3/18:2n-6, 20:5n-3/20:4n-6, 14:0, 16:1n-7, and 18:1n-7. Embryos produced by pairs fed ANArg exhibited delayed hatching but developed into larvae with the highest survival. Changes in ova FA and sperm quality indicate that, in zebrafish, gametes are directly influenced by parental diet, making the species an effective rapid screening system. Diet-induced changes in ova FA resulted in significant effects on hatchability, larval body morphometrics (BM), and survival. These findings indicate that zebrafish performance across embryonic-larval stages provides a sensitive model for screening POs, with survival serving as a conclusive endpoint. Survival was primarily linked to the ratio between LC-PUFAs and other FAs in ova. POs promoting these interactions are superior for aquafeeds. These ratios also guide broodstock diets to improve larval success and nutritional programming.

为了评价斑马鱼作为水产饲料植物油（POs）筛选系统的有效性，我们用Koroneiki、Parseh和Arghavan三种单一品种橄榄油（OO）富集了Artemia nauplii （AN）。然后将得到的AN （ANKor、ANPar、ANArg和AN36[饥饿36 h的AN，对照]）喂给360条鱼（3.5 cm）一个月。AN的脂肪酸谱反映在卵子中，影响精子的活力和密度，进而影响受精率。ANKor组受精率最高，孵化速度加快。在该组中，鱼产生的精子活力最高，卵子的18:1n- 9,22:6 n-3/20:5n-3含量较高，18:3n-3/18:2n-6、20:5n-3/20:4n-6、14:0、16:1n-7和18:1n-7含量较低。配对饲喂ANArg产生的胚胎孵化延迟，但发育成幼虫存活率最高。卵子FA和精子质量的变化表明，在斑马鱼中，配子直接受到亲代饮食的影响，使该物种成为一个有效的快速筛选系统。饮食诱导的卵FA变化对孵化率、幼虫体形态计量学（BM）和存活率有显著影响。这些发现表明，斑马鱼在胚胎-幼虫阶段的表现为筛选POs提供了一个敏感的模型，生存是一个决定性的终点。存活主要与卵子中LC-PUFAs和其他FAs的比例有关。促进这些相互作用的POs对于水产饲料来说是优越的。这些比例还可以指导种鱼的饮食，以提高幼虫成功率和营养规划。

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引用次数: 0

Taurine in Ruditapes philippinarum: tissue distribution, developmental dynamics and metabolic mechanism 菲律宾蛤的牛磺酸：组织分布、发育动力学和代谢机制。

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-10-23 DOI: 10.1016/j.cbpb.2025.111170

Yang Liu , Kai Liao , Yang Zhang , Qi Hu , Xiaoxue He , Fengjun Xie , Jilin Xu

Bivalves, although often overlooked as a source of taurine in the diet, have tissue levels of taurine that are notably higher than those in most vertebrates. However, the handling and metabolism of taurine in bivalves is still poorly understood. In the present study, we analyzed the tissue distribution of taurine in the clam Ruditapes philippinarum and evaluated the transcription of genes involved in its metabolism. Taurine concentrations differed significantly among tissues, with the gill displaying tissue concentrations more than three times higher than those in the digestive gland. Furthermore, it was demonstrated that taurine concentrations in microalgal feeds commonly employed in aquaculture are low. Clams may mitigate this deficiency by progressively accumulating taurine or related amino acids through prolonged consumption of microalgae. Additionally, our results confirmed that clams maintain continuous taurine biosynthesis throughout development, with the highest expression of taurine biosynthesis-related genes observed at the pediveliger stage. The amino acid composition of clams demonstrated significant dynamic variations throughout their development, with fluctuations in taurine precursors (cysteine, methionine, and serine) closely aligning with the expression patterns of genes involved in taurine biosynthesis. Collectively, these findings offer new insights into the tissue distribution of taurine in bivalves, emphasizing the significance of sustained taurine biosynthesis and accumulation in supporting their developmental processes and physiological functions.

双壳类动物虽然经常被忽视为饮食中牛磺酸的来源，但其组织中牛磺酸的含量明显高于大多数脊椎动物。然而，双壳类动物对牛磺酸的处理和代谢仍然知之甚少。本研究分析了菲律宾蛤（Ruditapes philippinarum）中牛磺酸的组织分布，并对其代谢相关基因的转录进行了评价。不同组织的牛磺酸浓度差别很大，鳃的牛磺酸浓度是消化腺的三倍以上。此外，水产养殖中常用的微藻饲料中的牛磺酸浓度较低。蛤蜊可以通过长时间食用微藻逐渐积累牛磺酸或相关氨基酸来减轻这种缺陷。此外，我们的研究结果证实，蛤在整个发育过程中保持持续的牛磺酸生物合成，在幼体发育阶段观察到牛磺酸生物合成相关基因的最高表达。蛤的氨基酸组成在其发育过程中表现出显著的动态变化，牛磺酸前体（半胱氨酸、蛋氨酸和丝氨酸）的波动与牛磺酸生物合成相关基因的表达模式密切相关。总的来说，这些发现为双壳类动物牛磺酸的组织分布提供了新的见解，强调了牛磺酸的持续生物合成和积累在支持其发育过程和生理功能中的重要性。

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引用次数: 0

Delta/notch signaling regulates blastema formation during limb regeneration in Eriocheir sinensis Delta/notch信号调控中华绒螯蟹肢体再生过程中胚基形成。

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-10-19 DOI: 10.1016/j.cbpb.2025.111168

Hao Li , Weijia Liu , Lei Zhu , Libo Hou , Xianghui Kong

Limb autotomy and regeneration represent specialized adaptive responses employed by crustaceans to cope with threats from other animals or environmental stress. However, the molecular regulatory mechanisms underlying these adaptations remain poorly understood. In this study, we established a limb autotomy and regeneration model in Eriocheir sinensis, characterized in detail the morphological and structural features of regenerated limbs post-amputation, analyzed expression patterns of key components of the Notch signaling pathway genes, and evaluated their functional roles during limb regeneration utilizing long-term RNA interference (RNAi) technology. Specifically, the regenerative blastema appeared approximately 14 days post- amputation, with both the receptor EsNotch and the ligand EsDelta expressed across distinct stages of limb regeneration and exhibiting distinct expression patterns. Notably, both genes were significantly up-regulated during the blastema growth stage. Subsequently, we observed that long-term knockdown of either EsNotch or EsDelta significantly suppressed the formation of blastema during limb regeneration and increased crab mortality. Furthermore, we found that their silencing triggered the down-regulation of genes involved in cell proliferation, differentiation, and other regeneration-related processes. Overall, our findings underscore the critical functional role of Notch signaling in crab limb regeneration via modulation of regeneration-related gene expression, significantly advancing understanding of molecular mechanisms underlying crustacean limb regeneration and paving the way for future regenerative biology research.

肢体自切和再生是甲壳类动物为应对来自其他动物的威胁或环境压力而采取的特殊适应反应。然而，这些适应的分子调控机制仍然知之甚少。本研究建立了中华绒螯蟹自断肢体再生模型，详细表征了截肢后再生肢体的形态和结构特征，分析了Notch信号通路关键组分基因的表达模式，并利用长时程RNA干扰（RNAi）技术评估了其在肢体再生中的功能作用。具体来说，再生胚在截肢后大约14天出现，受体EsNotch和配体EsDelta在肢体再生的不同阶段表达，并表现出不同的表达模式。值得注意的是，这两个基因在胚芽生长阶段均显著上调。随后，我们观察到，长期敲低EsNotch或EsDelta显著抑制了螃蟹肢体再生过程中囊胚的形成，并增加了螃蟹的死亡率。此外，我们发现它们的沉默触发了参与细胞增殖、分化和其他再生相关过程的基因的下调。总之，我们的研究结果强调了Notch信号通过调控再生相关基因表达在螃蟹肢体再生中的关键功能作用，显著推进了对甲壳类动物肢体再生分子机制的理解，并为未来的再生生物学研究铺平了道路。

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