Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology最新文献_第7页

Fungal disease does not affect skin carotenoid concentrations in dietary-supplemented strawberry poison frogs 真菌病不影响草莓毒蛙的皮肤类胡萝卜素浓度。

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-06-02 DOI: 10.1016/j.cbpb.2025.111113

Rachel K. Prokopius , Kevin J. McGraw , Alessandro Catenazzi

Carotenoids are colored molecules that are sequestered through diet and provide health benefits and affect reproductive success in certain organisms. Batrachochytrium dendrobatidis (Bd) is a worldwide amphibian infection that invades skin, disrupts vital regulatory processes and can cause death. We hypothesized Bd skin infection affects sequestration of important skin carotenoids in carotenoid-supplemented strawberry poison frogs (Oophaga pumilio). We predicted that infection would deplete skin carotenoid concentrations due to the energetic or immunological trade-off required when fighting infection. We experimentally infected a group of adult male and female O. pumilio with Bd and used high-performance liquid chromatography (HPLC) to compare types and concentrations of 17 unique skin carotenoids between infected and control (mock-infected) animals that were all supplemented with dietary carotenoids. We achieved a range of Bd infection (from around 1.0 × 10¹ to above 1.0 × 10⁷ zoospore equivalents) in all infected frogs. We found no effect of Bd infection on skin carotenoid concentrations, regardless of sex. This suggests Bd infection is not sufficient to alter skin carotenoids in O. pumilio with non-limited dietary carotenoids, perhaps due to sufficient energy available both for combating infection and maintaining skin carotenoids in captive animals. We suggest further study of the energetics of sequestering and metabolizing carotenoids in amphibians and its relationship to energy needed when combating Bd infection to better understand how skin carotenoids are maintained when Bd infection takes hold. Studying the effect of pathogen infection on organism carotenoid sequestration will continue to be important as emerging infectious diseases surface worldwide.

类胡萝卜素是通过饮食隔离的有色分子，对某些生物体有益健康，并影响其繁殖成功率。树突壶菌（Bd）是一种世界范围的两栖动物感染，它侵入皮肤，破坏重要的调节过程并可导致死亡。我们假设Bd皮肤感染会影响补充类胡萝卜素的草莓毒蛙（Oophaga pumilio）对重要皮肤类胡萝卜素的吸收。我们预测感染会耗尽皮肤类胡萝卜素浓度，因为在对抗感染时需要能量或免疫权衡。我们实验用Bd感染了一组成年雄性和雌性pumilio，并使用高效液相色谱法（HPLC）比较了17种独特的皮肤类胡萝卜素的类型和浓度，感染动物和对照组（模拟感染）都补充了膳食类胡萝卜素。我们在所有受感染的青蛙中获得了Bd感染范围（从1.0 × 101左右到1.0 × 107以上的虫孢子当量）。我们发现，无论性别，Bd感染对皮肤类胡萝卜素浓度没有影响。这表明，不限制饮食中的类胡萝卜素的情况下，Bd感染并不足以改变pumilio的皮肤类胡萝卜素，这可能是由于圈养动物有足够的能量来对抗感染和维持皮肤类胡萝卜素。我们建议进一步研究两栖动物吸收和代谢类胡萝卜素的能量学及其与对抗Bd感染时所需能量的关系，以更好地了解当Bd感染发生时皮肤类胡萝卜素是如何维持的。随着世界范围内新出现的传染病的出现，研究病原体感染对生物类胡萝卜素吸收的影响将继续具有重要意义。

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引用次数: 0

Genome-wide identification of heat shock protein 90 family in Larimichthys crocea and expression analysis in response to thermal stress and Vibrio parahaemolyticus infection 大鲵热休克蛋白90家族的全基因组鉴定及对热应激和副溶血性弧菌感染的表达分析

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-05-19 DOI: 10.1016/j.cbpb.2025.111112

Xinxin Chen , Zebin Chen , Yuqing Xu , Pengfei Zou , Weiliang Shen , Ziping Zhang , Yilei Wang

Members of the heat shock protein 90 family (HSP90s) are evolutionarily conserved and play crucial roles in protein transport, immune regulation and antigen presentation. In this study, five hsp90s were identified from the genome of large yellow croaker (Larimichthys crocea) and analyzed using bioinformatics. All five identified hsp90s encode proteins with HATPase_c and HSP90 domains, and are mainly localized in the cytoplasm, mitochondria and endoplasmic reticulum. Chromosomal mapping revealed their distribution across three distinct chromosomes. Quantitative real-time PCR (qPCR) analysis showed differential expression patterns of the five hsp90s in 11 tissues. Additionally, their expression dynamics in the liver, spleen, head kidney, gill and blood were analyzed at 3 h, 12 h, 24 h and 48 h post thermal stress, Vibrio parahaemolyticus infection or under a combination of these two stressors. Results showed that the L. crocea hsp90s exhibited distinct expression patterns in response to the above three stimuli in different immune tissues. Notably, hsp90s in the spleen were most responsive. This study systematically clarified for the first time the gene structure characteristics, tissue expression patterns, and environmental stress response mechanisms of the HSP90 family in L. crocea. It confirmed that hsp90s show significant functional differentiation and synergy in response to biotic (pathogen infection) and abiotic (thermal stress) stresses, and provides important clues for a deeper understanding of the genetic basis of environmental adaptation in L. crocea.

热休克蛋白90家族（hsp90）的成员在进化上是保守的，在蛋白质转运、免疫调节和抗原呈递中起着至关重要的作用。本研究从大黄鱼（Larimichthys crocea）基因组中鉴定出5个hsp90，并进行了生物信息学分析。这5种HSP90均编码具有HATPase_c和HSP90结构域的蛋白，主要定位于细胞质、线粒体和内质网。染色体作图显示它们分布在三条不同的染色体上。实时荧光定量PCR （qPCR）分析显示5种hsp90在11个组织中的表达差异。此外，在热应激、副溶血性弧菌感染或两种应激因素共同作用下，分析了它们在肝脏、脾脏、头肾、鳃和血液中的表达动态。结果表明，L. crocea hsp90在不同免疫组织中对上述三种刺激表现出不同的表达模式。值得注意的是，脾脏中的hsp90反应最明显。本研究首次系统阐明了crocea HSP90家族的基因结构特征、组织表达模式和环境胁迫反应机制。这证实了hsp90s在生物（病原体感染）和非生物（热应激）胁迫下表现出显著的功能分化和协同作用，为深入了解水藻环境适应的遗传基础提供了重要线索。

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引用次数: 0

Hepatic protective effects and oxidative stress modulation via gene expression in zebrafish (Danio rerio) fed with Synechococcus elongatus PCC 7942 as a functional feed additive 长聚球菌PCC 7942作为功能性饲料添加剂对斑马鱼肝脏保护作用及氧化应激的基因表达调控

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-05-11 DOI: 10.1016/j.cbpb.2025.111111

Mirna Leandra Enriquez Reyes , Andrea Idelette Hernandez , Raíza dos Santos Azevedo , Beatriz Xavier Figueiredo , Isaac dos Santos Flores , Arthur Cardoso , Tony Silveira , Iuri Salim Abou Anni , Antonio Sergio Varela Junior , Bruna Felix Nornberg , Luis Fernando Marins

The inclusion of cyanobacteria in aquafeeds is a sustainable alternative to traditional fishmeal. This study evaluated the effects of Synechococcus elongatus PCC 7942 supplementation on intestinal morphology, liver histopathology, and antioxidant gene expression in zebrafish (Danio rerio). Fish were fed a commercial diet (CF) or the same diet supplemented with S. elongatus (EF) for 35 days. Liver histopathology revealed that 62 % of fish in the CF group exhibited generalized liver alteration, while fish in the EF group showed a lower frequency of generalized alteration (31 %) and a higher frequency of multifocal lesions (46 %), suggesting improved hepatic homeostasis. Intestinal morphometry showed no significant changes in villus length between groups. Gene expression analysis demonstrated a significant downregulation of xenobiotic metabolism genes (cyp1a, gst), antioxidant defense genes (sod1, sod2, cat), and steroid metabolism (cyp19a1a) in fish fed S. elongatus, except for gpx, which remained unchanged. The reduction in antioxidant gene expression, along with improved liver histology, suggests a lower oxidative stress in the EF group, likely due to synergistic effects of S. elongatus in mitigating oxidative damage. These findings indicate that S. elongatus supplementation does not impair intestinal morphology or liver function but supports hepatic homeostasis by reducing oxidative stress and modulating liver histopathology. This highlights its potential as a functional feed additive in aquaculture.

在水产饲料中加入蓝藻是传统鱼粉的可持续替代品。本研究评价了添加长聚球菌PCC 7942对斑马鱼肠道形态、肝脏组织病理学和抗氧化基因表达的影响。分别饲喂商品饲料（CF）和在相同饲料中添加长形棘鱼（EF） 35 d。肝脏组织病理学显示，CF组62%的鱼表现出全身性肝脏改变，而EF组的鱼表现出较低的全身性改变频率（31%）和较高的多灶性病变频率（46%），表明肝脏稳态得到改善。肠道形态测定显示各组间绒毛长度无明显变化。基因表达分析显示，除了gpx保持不变外，长形梭鱼的外源代谢基因（cyp1a、gst）、抗氧化防御基因（sod1、sod2、cat）和类固醇代谢基因（cyp19a1a）均显著下调。抗氧化基因表达的减少，以及肝脏组织学的改善，表明EF组的氧化应激较低，可能是由于长形葡萄球菌在减轻氧化损伤方面的协同作用。这些研究结果表明，长形葡萄球菌的补充不会损害肠道形态或肝功能，而是通过减少氧化应激和调节肝脏组织病理学来支持肝脏稳态。这突出了其作为水产养殖功能性饲料添加剂的潜力。

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引用次数: 0

Exploring the role of miR-430 in hybrid fish during embryonic development 探讨miR-430在杂交鱼胚胎发育中的作用。

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-05-09 DOI: 10.1016/j.cbpb.2025.111110

Yirui Zhang , Chang Wang , Jiahao Wu, Ting Liu, Han Wu, Zhonghua Peng, Chengxi Liu, Shengwei Wang, Yan Wang, Kaikun Luo, Jing Wang, Shaojun Liu

miR-430, a microRNA expressed at the maternal-zygotic transition (MZT) stage, plays a vital role in maternal transcript clearance and suppression of primordial germ cell-specific genes. This study investigated the expression and regulation of miR-430 in goldfish (Carassius auratus var., ♀) × rare gudgeon (Gobiocypris rarus, ♂) [s-GFRG, survival] and rare gudgeon (♀) × goldfish (♂) [d-RGGF, death] embryos to explore the role of miR-430 in hybrid fish. Gene sequence comparisons demonstrated that three types of miR-430 in s-GFRG exhibited similarity to that of the female parent goldfish (GF) and displayed characteristic variation. Conversely, d-RGGF exhibited two miR-430 variants resembling those of GF and rare gudgeon (RG). In addition, real-time quantitative PCR and whole-mount in situ hybridization revealed that the expression trend of miR-430 was the same in hybrid progenies, and temporal expression was delayed compared to that in the parental embryos. However, miR-430 expression was significantly lower in d-RGGF than in GF and s-GFRG embryos. Similar to the development of d-RGGF embryos, miR-430-silenced s-GFRG embryos exhibited morphological abnormalities including spinal curvature and pericardial cavity enlargement. Overexpression of miR-430 in d-RGGF embryos effectively rescued somitogenesis and prolonged fry survival. Thus, an abnormal MZT resulting from disturbed miR-430 expression may contribute to hybrid embryo mortality.

miR-430是一种在母系-合子过渡（MZT）阶段表达的microRNA，在母体转录物清除和原始生殖细胞特异性基因抑制中起着至关重要的作用。本研究通过研究miR-430在金鱼（Carassius auratus var.，♀） × 稀有鲟（Gobiocypris rarus，♂）[s-GFRG， survival]和稀有鲟（♀） × 金鱼（♂）[d-RGGF， death]胚胎中的表达和调控，探讨miR-430在杂交鱼中的作用。基因序列比较表明，s-GFRG中三种类型的miR-430与雌性亲本金鱼（GF）相似，并表现出特征性变异。相反，d-RGGF表现出两种类似GF和RG的miR-430变体。real-time定量PCR和全载原位杂交结果显示，miR-430在杂交后代中的表达趋势相同，且时间表达较亲本胚胎延迟。然而，miR-430在d-RGGF中的表达明显低于GF和s-GFRG胚胎。与d-RGGF胚胎的发育类似，mir -430沉默的s-GFRG胚胎表现出包括脊柱弯曲和心包腔增大在内的形态学异常。在d-RGGF胚胎中过表达miR-430可有效挽救体细胞发生并延长鱼苗存活时间。因此，miR-430表达紊乱导致的MZT异常可能导致杂交胚胎死亡。

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引用次数: 0

Molecular characterization and expression analysis of interleukin-1 beta in Japanese eel (Anguilla japonica) 日本鳗鲡（Anguilla japonica）白细胞介素-1 β的分子特征及表达分析

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-29 DOI: 10.1016/j.cbpb.2025.111102

I-Pei Kuo , Yu-Ting Chu , Ching-Shuo Liu , Shuenn-Der Yang , Fan-Hua Nan

Interleukin 1-beta (IL-1B) is a key proinflammatory cytokine involved in disease resistance. In recent years, il-1β genes from several teleosts have been cloned. The Japanese eel (Anguilla japonica) is an evolutionarily primitive fish widely farmed in East Asia. The il-1β gene has not yet been cloned from Japanese eel. In this study, the complete cDNA of il-1β was successfully sequenced from peripheral leukocytes through the rapid amplification of cDNA ends. The il-1β gene consists of five exons and four introns, and the full-length cDNA spans 1204 bp, comprising a 99-bp 5′ untranslated region, a 750-bp coding sequence, and a 355-bp 3′ untranslated region. The deduced amino acid sequence includes a consensus IL-1 family signature but lacks both a signal peptide and an IL-1 converting enzyme cleavage site, similar to other teleost IL-1B proteins. Homology analysis revealed that Japanese eel IL-1B is highly conserved within the order Anguilliformes, sharing the highest similarity with American eel (Anguilla rostrata), followed by conger eel (Conger myriaster). Tissue expression analysis showed that il-1β is constitutively expressed in multiple tissues, with high expression in peripheral leukocytes and the spleen; moderate expression in the gill, liver, head kidney, trunk kidney, and intestine; and low expression in the heart, stomach, skin, and muscle. In vitro stimulation with zymosan, polyinosinic-polycytidylic acid, and lipopolysaccharide upregulated il-1β expression in peripheral leukocytes.

白细胞介素1- β (IL-1B)是参与疾病抵抗的关键促炎细胞因子。近年来，已经克隆了几种硬骨鱼的il-1β基因。日本鳗鲡（Anguilla japonica）是一种在东亚广泛养殖的进化原始鱼类。il-1β基因尚未从日本鳗鱼中克隆出来。本研究通过对il-1β cDNA末端的快速扩增，成功测序了外周白细胞il-1β的完整cDNA。il-1β基因由5个外显子和4个内含子组成，全长全长1204 bp，包括一个99 bp的5 ‘非翻译区、一个750 bp的编码序列和一个355 bp的3 ’非翻译区。推断的氨基酸序列包括一致的IL-1家族特征，但缺乏信号肽和IL-1转换酶切割位点，类似于其他硬骨鱼IL-1B蛋白。同源性分析表明，日本鳗鲡IL-1B在鳗鲡目中具有高度保守性，与美洲鳗鲡（Anguilla rostrata）的相似性最高，其次是长鳗（conger myriaster）。组织表达分析显示，il-1β在多组织中组成性表达，在外周血白细胞和脾脏中高表达；在鳃、肝、头肾、干肾和肠中有中等表达；在心脏、胃、皮肤和肌肉中表达较低。酶聚糖、多肌苷-多胞酸和脂多糖体外刺激可上调外周白细胞中il-1β的表达。

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引用次数: 0

Regulatory variants of glycogen synthase contribute to differential glycogen content between Crassostrea gigas and C. sikamea 糖原合成酶的调节变异导致长牡蛎和狐獴之间糖原含量的差异

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-26 DOI: 10.1016/j.cbpb.2025.111101

Ying Tan , Chenyu Shi , Qi Li , Hongqiang Xu , Shikai Liu

In marine bivalves, glycogen functions as a key energy source, influencing essential physiological processes such as growth, development, gametogenesis, and the reproductive cycle. Glycogen synthase (GYS), the key enzyme regulating glycogen biosynthesis, shows expression patterns directly correlated with seasonal glycogen fluctuations. This study identified genetic variations within the transcriptional regulatory region of the GYS gene in populations of Crassostrea gigas and C. sikamea. Two key single nucleotide polymorphism (SNP) sites, SNP -792-G/T and SNP -60-T/C, were identified to be associated with glycogen content. The polymorphisms in the GYS gene promoter result in species-specific differences in transcriptional binding activity, with C. sikamea showing higher activity in core region compared to C. gigas. Polymorphisms in this highly conserved promoter region alter the affinity of transcription regulatory elements for hypoxia-inducible factor 1 alpha (HIF-1α) and octamer-binding transcription factor 1 (OCT-1), thereby influencing GYS gene expression levels. These changes were related with interspecific differences in glycogen content between C. sikamea and C. gigas. Experimental evidence confirmed the specific binding of transcription factors to SNP-mutated promoter motifs. These SNPs represent critical species-specific regulatory sites and may serve as valuable markers for selecting oyster strains with high glycogen content. Our findings demonstrate that SNPs within the transcription regulatory elements of the GYS gene alter the binding affinity for transcription factors OCT-1 and HIF-1α, thereby playing a critical role in energy metabolism in oysters. Our study offers novel insights into the transcriptional regulation of the GYS gene and its contribution to glycogen storage.

在海洋双壳类动物中，糖原是一种重要的能量来源，影响着生长、发育、配子体发生和生殖周期等重要生理过程。糖原合成酶（GYS）是调节糖原生物合成的关键酶，其表达模式与糖原的季节波动直接相关。本研究确定了长牡蛎和狐蝠种群中GYS基因转录调控区域内的遗传变异。两个关键的单核苷酸多态性位点SNP -792-G/T和SNP -60-T/C与糖原含量相关。GYS基因启动子的多态性导致了物种特异性转录结合活性的差异，与C. gigas相比，C. sikamea在核心区域表现出更高的活性。这个高度保守的启动子区域的多态性改变了转录调控元件对缺氧诱导因子1α (HIF-1α)和八聚体结合转录因子1 （OCT-1）的亲和力，从而影响GYS基因的表达水平。这些变化与狐獴和巨蝉种间糖原含量的差异有关。实验证据证实了转录因子与snp突变的启动子基序的特异性结合。这些snp代表了关键的物种特异性调控位点，可能作为选择高糖原含量牡蛎菌株的有价值的标记。我们的研究结果表明，GYS基因转录调控元件内的snp改变了转录因子OCT-1和HIF-1α的结合亲和力，从而在牡蛎的能量代谢中发挥关键作用。我们的研究为GYS基因的转录调控及其对糖原储存的贡献提供了新的见解。

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引用次数: 0

Effects of dietary oregano essential oil on digestive tissue structure and function, antioxidant and immune responses and gut microbiota of red swamp crayfish (Procambarus clarkii) 饲粮中添加牛至精油对克氏原螯虾消化组织结构和功能、抗氧化和免疫反应及肠道微生物群的影响

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-21 DOI: 10.1016/j.cbpb.2025.111100

Xinzi Guo , Zhaoxuan Zhu , Hanxiao Wang , Yue Chen , Songrun Zhang , Dian Wang , Xingxing Fang , Ping Yang

Modifying the diet is an effective strategy to enhance the production and health of farmed red swamp crayfish (Procambarus clarkii). In this study the effects of adding dietary oregano essential oil (OEO) at 0, 100, 300, and 500 mg/kg concentrations were investigated in red swamp crayfish after a 3-week feeding trial. Histology showed no negative impacts on the structural integrity of the hepatopancreas and intestine. Total protease activity in the hepatopancreas, hemolymph alkaline and acid phosphatase activity, hemolymph superoxide dismutase activity and hepatopancreas glutathione levels were higher in the 500 mg/kg OEO group than in the 0 mg/kg group. No significant differences were observed in hepatopancreas phenoloxidase activity or hemolymph malondialdehyde content between treatment groups. Supplementing the diet with OEO upregulated the expression of antioxidant and immune-related genes in the hepatopancreas including metallothionein, gamma interferon-inducible lysosomal thiol reductase, and catalase in the 500 mg/kg OEO group relative to the 0 mg/kg control. Ferritin gene expression was unaltered. The intestinal microbiota of crayfish fed OEO showed an increased relative abundance of Proteobacteria and Actinobacteriota, and a lower abundance of Firmicutes. This study demonstrates the potential of OEO as a novel feed additive for enhancing antioxidant capacity and the immune response in red swamp crayfish. This research addresses the growing need for sustainable aquaculture practices and highlights the application of plant-derived bioactivities in improving crustacean health.

改变饲料是提高克氏原螯虾（Procambarus clarkii）产量和健康的有效策略。本试验通过3周的饲养试验，研究了在红沼泽小龙虾中添加0、100、300和500 mg/kg浓度的牛至精油（OEO）的效果。组织学显示对肝胰腺和肠的结构完整性没有负面影响。500 mg/kg OEO组肝胰脏总蛋白酶活性、血淋巴碱性和酸性磷酸酶活性、血淋巴超氧化物歧化酶活性和肝胰脏谷胱甘肽水平均高于0 mg/kg组。肝胰腺酚氧化酶活性和血淋巴丙二醛含量在治疗组间无显著差异。与0 mg/kg对照组相比，500 mg/kg OEO组肝胰腺中金属硫蛋白、γ干扰素诱导溶酶体硫醇还原酶和过氧化氢酶等抗氧化和免疫相关基因的表达上调。铁蛋白基因表达未见改变。小龙虾肠道菌群中变形菌门和放线菌门的相对丰度增加，厚壁菌门的相对丰度降低。本研究证明了OEO作为一种新型饲料添加剂在提高红沼泽小龙虾抗氧化能力和免疫应答方面的潜力。本研究解决了对可持续水产养殖实践日益增长的需求，并强调了植物源性生物活性在改善甲壳类动物健康方面的应用。

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引用次数: 0

Integrative whole-genome methylation and transcriptome analysis reveals epigenetic modulation of glucose metabolism by dietary berberine in blunt snout bream (Megalobrama amblycephala) 全基因组甲基化和转录组整合分析揭示钝口鳊鱼（Megalobrama amblycephala）膳食小檗碱对葡萄糖代谢的表观遗传调节作用

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-16 DOI: 10.1016/j.cbpb.2025.111098

Xiaoyan Jia, Wei Qiang, Le Chang, Kang Xiao, Ronghua Zhou, Qiyong Qiu, Guangzhen Jiang, Xiangfei Li, Cheng Chi, Wenbin Liu, Dingdong Zhang

The present research was designed to explore the epigenetic mechanism by which dietary berberine (BBR) affects glucose metabolism in fish. Blunt snout bream (Megalobrama amblycephala) is susceptible to disturbances in glucose metabolism when subjected to prolonged high-carbohydrate diets. This study aimed to elucidate whether BBR can enhance glucose regulation in M. amblycephala via modulating DNA methylation levels. Fish (average weight of 20.36 ± 1.44 g) were administered a normal-carbohydrate diet (NC, 30 % carbohydrate), a high-carbohydrate diet (HC, 43 % carbohydrate), or a high-carbohydrate diet supplemented with 50 mg/kg berberine (HB) for 10 weeks. Subsequently, global DNA methylation level, whole-genome bisulfite sequencing (WGBS), RNA-seq, bisulfite sequencing PCR, and real-time quantitative PCR were employed to analyze the DNA methylation patterns and transcription results of the liver genome. The findings indicated that high carbohydrate diets induced glucose metabolism disorders in M. amblycephala, whereas BBR mitigated these metabolic disturbances by reducing methylation levels. WGBS results revealed that CG-type cytosine methylation predominated, and that DNA methylation mainly occurred in promoter, intron, and exon regions. Furthermore, analyses demonstrated a negative correlation between DNA methylation around the transcriptional start site and gene expression levels for 47 genes. Functional enrichment analysis revealed that these genes were associated with 60 KEGG pathways, including 12 genes implicated in the amelioration of insulin resistance, reduction of gluconeogenesis, and maintenance of glucose homeostasis. Consequently, we generated a comprehensive catalog of liver DNA methylation in M. amblycephala, which provides a foundational framework for future investigations into the epigenetic regulation of glucose metabolism by BBR.

本研究旨在探讨饲料中小檗碱（BBR）影响鱼类葡萄糖代谢的表观遗传机制。钝口鲷（Megalobrama amblycephala）在长期高碳水化合物饮食时易受葡萄糖代谢紊乱的影响。本研究旨在阐明BBR是否可以通过调节DNA甲基化水平来增强双头m.s amblycephala的葡萄糖调节。鱼（平均体重20.36±1.44 g）被给予正常碳水化合物饮食（NC， 30%碳水化合物），高碳水化合物饮食（HC， 43%碳水化合物），或高碳水化合物饮食中添加50 mg/kg小檗碱（HB），为期10周。随后，采用全局DNA甲基化水平、全基因组亚硫酸氢盐测序（WGBS）、RNA-seq、亚硫酸氢盐测序PCR和实时定量PCR分析肝脏基因组DNA甲基化模式和转录结果。研究结果表明，高碳水化合物饮食会引起双头m.a blblycephala的糖代谢紊乱，而BBR通过降低甲基化水平来减轻这些代谢紊乱。WGBS结果显示cg型胞嘧啶甲基化占主导地位，DNA甲基化主要发生在启动子、内含子和外显子区域。此外，分析表明47个基因转录起始位点附近的DNA甲基化与基因表达水平呈负相关。功能富集分析显示，这些基因与60个KEGG通路相关，其中12个基因与改善胰岛素抵抗、减少糖异生和维持葡萄糖稳态有关。因此，我们生成了一个全面的双头m.b amblycephala肝脏DNA甲基化目录，这为未来研究BBR对葡萄糖代谢的表观遗传调控提供了基础框架。

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引用次数: 0

Effects of Vibrio alginolyticus on intestinal health and intestinal flora of sea urchin (Strongylocentrotus intermedius) 藻溶弧菌对海胆（Strongylocentrotus intermedius）肠道健康和肠道菌群的影响

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-16 DOI: 10.1016/j.cbpb.2025.111099

Xiaochen Zhang , Wei Chen , Haoyu Zhang , Yan Li , Yijing Han , Wan Liu , Yaqiong Liu , Xiaona Wang , Xuekai Zhang , Deyang Tian , Xiaotong Wang

The aim of this study was to understand the effect of Vibrio alginolyticus on the intestinal tract of Strongylocentrotus intermedius. The effects of injecting V. alginolyticus into the body cavity via the perioral membrane at concentrations of 0 CFU/mL (C), 1.5 × 10⁷ CFU/mL (VA1), and 1.5 × 10⁸ CFU/mL (VA10) on the intestinal tract of S. intermedius were analyzed using histological examination, immunoenzyme activity, and 16S rRNA sequencing. The results showed that V. alginolyticus caused intestinal tissue damage and oxidative stress (e.g. altered levels of superoxide dismutase). In addition, the intestinal flora was altered. At the phylum level, the abundance of Bacteroidota was significantly decreased in the VA10 group, at the genus level, Vibrio spp. exhibited a significant increase following V. alginolyticus injection. Prediction of Kyoto Encyclopedia of Genes and Genomes function in the intestinal flora revealed that high concentrations of V. alginolyticus may have induced pathways such as energy metabolism. These results indicated that V. alginolyticus caused lesions in the intestinal morphology of S. intermedius and disrupted the balance of intestinal flora.

本研究旨在了解溶藻弧菌对中间圆心弧菌肠道的影响。采用组织学检查、免疫酶活性、16S rRNA测序等方法分析了浓度为0 CFU/mL (C)、1.5 × 107 CFU/mL （VA1）、1.5 × 108 CFU/mL （VA10）的溶藻弧菌经口周膜体腔注射对中间链球菌肠道的影响。结果表明，溶藻弧菌引起肠道组织损伤和氧化应激（如超氧化物歧化酶水平改变）。此外，肠道菌群也发生了改变。在门水平上，VA10组拟杆菌门的丰度显著降低；在属水平上，溶藻弧菌组弧菌门的丰度显著增加。《京都基因与基因组百科全书》对肠道菌群功能的预测表明，高浓度的溶藻弧菌可能具有能量代谢等诱导途径。这些结果表明，溶藻弧菌引起了中间葡萄球菌肠道形态的病变，破坏了肠道菌群的平衡。

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引用次数: 0

Effect of prebiotics added to the culture water on the bacterial profiles of biofloc and the gills, hepatopancreas, and intestine of Penaeus vannamei 在培养水中添加益生元对凡纳滨对虾生物群落及鳃、肝胰腺和肠道细菌分布的影响。

IF 1.9 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-04-01 DOI: 10.1016/j.cbpb.2025.111097

Misael Rosales-Leija , Delbert M. Gatlin III , Jonathan P. Holt , Addison L. Lawrence

Prebiotics alter microbial communities by supplying carbon to specific bacteria that benefit the host. However, the effect of prebiotics added to the culture water on shrimp production and bacterial composition has not been demonstrated. Therefore, this study evaluated the effects of four prebiotics — short-chain fructooligosaccharide, galactooligosaccharide, inulin, and mannan-oligosaccharide — and two non-prebiotic carbohydrates — sucrose and wheat starch — on shrimp growth, nutrient composition of shrimp muscle and biofloc, biofloc concentration, and bacterial profiles in biofloc and gills, hepatopancreas, and intestine of Penaeus vannamei. Shrimp (6.0 ± 0.5 g) were stocked into 36 tanks (72 shrimp/m²) and raised in a biofloc technology system consisting of two phases: an autotrophic-dominated phase (days 0–4) and a heterotrophic-dominated phase (days 5–26). The prebiotic and non-prebiotic carbohydrates were added to the water at a rate of 3 % of the feed provided. Key findings revealed that adding prebiotics to the water did not affect shrimp growth. Notably, shrimp muscle from the mannan-oligosaccharide treatment exhibited a significantly higher lipid content than that from the galactooligosaccharide treatment (p ≤ 0.05). No significant effect was observed on the nutrient composition of biofloc. Biofloc concentration was lower in the wheat starch treatment (p ≤ 0.05). Finally, the prebiotics altered the bacterial composition of biofloc and shrimp gills, hepatopancreas, and intestine. These results revealed that adding prebiotics to the culture water of a biofloc system has an effect on shrimp quality and microbial communities of biofloc and various shrimp tissues, suggesting their potential benefit on shrimp production and health.

益生素通过向特定细菌提供碳来改变微生物群落，从而使宿主受益。然而，添加到养殖水中的益生元对对虾产量和细菌组成的影响尚未得到证实。因此，本研究评估了四种益生元（短链果寡糖、半乳寡糖、菊粉和甘露寡糖）和两种非益生元碳水化合物（蔗糖和小麦淀粉）对对虾生长、对虾肌肉和生物絮团的营养成分、生物絮团浓度以及生物絮团、对虾鳃、肝胰脏和肠道中细菌特征的影响。将对虾（6.0 ± 0.5 克）放入 36 个水箱（72 只/平方米），在生物絮凝技术系统中养殖，该系统包括两个阶段：自养为主的阶段（第 0-4 天）和异养为主的阶段（第 5-26 天）。益生菌和非益生碳水化合物按所提供饲料的 3% 的比例添加到水中。主要研究结果表明，在水中添加益生素不会影响虾的生长。值得注意的是，来自甘露寡糖处理的对虾肌肉的脂质含量明显高于来自半乳寡糖处理的对虾肌肉的脂质含量（p ≤ 0.05）。生物絮凝物的营养成分没有受到明显影响。小麦淀粉处理的生物絮凝物浓度较低（p ≤ 0.05）。最后，益生素改变了生物絮凝物和对虾鳃、肝胰腺和肠道的细菌组成。这些结果表明，在生物絮团系统的养殖水中添加益生素对对虾的质量以及生物絮团和对虾各种组织的微生物群落有影响，表明益生素对对虾的生产和健康有潜在的益处。

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引用次数: 0