Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology最新文献_第4页

Effects of salinity on the regulation of long-chain polyunsaturated fatty acid biosynthesis in the polychaete Platynereis dumerilii 盐度对多毛藻长链多不饱和脂肪酸生物合成调控的影响。

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-09-12 DOI: 10.1016/j.cbpb.2025.111164

Khalida Bainour, Óscar Monroig, Marc Ramos-Llorens, Juan C. Navarro

Polychaetes are promising sources of n-3 long-chain (≥C₂₀) polyunsaturated fatty acid (LC-PUFA) due to their ability to synthesise these essential nutrients through elongases, front-end desaturases (Fed), and methyl-end desaturases. Salinity can influence lipid metabolism in aquatic invertebrates by triggering osmotic stress that alters membrane lipid composition. This study investigates the effect of salinity on LC-PUFA biosynthesis in the polychaete, Platynereis dumerilii, a model organism in Evolutionary and Developmental Biology. In vivo trials were conducted with juveniles under standard conditions (18 °C, 16 L:8D photoperiod, frozen spinach as feed), evaluating three salinity levels: 30 ‰, 35 ‰, and 40 ‰. After one month, survival and growth were assessed, and samples were collected to evaluate the modulation of n-3 LC-PUFA biosynthesis by analysing total lipids, fatty acid (FA) profiles, and gene expression of elongases, desaturases, and transcription factors (TF). Despite a uniform diet, P. dumerilii at 40 ‰ salinity exhibited elevated levels of n-3 LC-PUFA (20:4n-3, 20:5n-3, 22:5n-3). Variations in FA profiles suggest salinity plays a regulatory role in lipid metabolism. Gene expression analysis showed significant upregulation of a Fed, with Δ6/Δ8 activity, under high salinity (40 ‰), consistent with reduced levels of its substrates, eicosadienoic acid (20:2n-6) and eicosatrienoic acid (20:3n-3). Additionally, the TF EcR (Ecdysone receptor) showed increased expression, suggesting involvement in LC-PUFA biosynthesis regulation. These findings indicate that cultivating P. dumerilii in high salinity may enhance its n-3 LC-PUFA content. Similarly, the nutritional value of other polychaetes, considered sustainable alternatives for aquaculture feed, may be improved through salinity modulation.

多毛动物是n-3长链（≥C20）多不饱和脂肪酸（LC-PUFA）的有希望的来源，因为它们能够通过延长酶、前端去饱和酶（Fed）和甲基端去饱和酶合成这些必需的营养物质。盐度可以通过触发改变膜脂组成的渗透应激来影响水生无脊椎动物的脂质代谢。本研究探讨了盐度对进化与发育生物学模式生物——多毛藻（Platynereis dumerilii）中LC-PUFA生物合成的影响。在标准条件下（18 °C， 16 L:8D光周期，冷冻菠菜为饲料）对幼鱼进行体内试验，评估三种盐度水平：30 ‰，35 ‰和40 ‰。1个月后，评估存活和生长情况，并收集样本，通过分析总脂质、脂肪酸（FA）谱以及延长酶、去饱和酶和转录因子（TF）的基因表达来评估n-3 LC-PUFA生物合成的调节。尽管饮食均匀，盐度为40 ‰时，P. dumerilii的n-3 LC-PUFA水平升高（20:4n- 3,20:5 n- 3,22:5 n-3）。FA谱的变化表明盐度在脂质代谢中起调节作用。基因表达分析显示，在高盐度（40 ‰）条件下，Fed活性显著上调（Δ6/Δ8），与其底物二十碳二烯酸（20:2n-6）和二十碳三烯酸（20:3n-3）水平降低一致。此外，TF EcR（蜕皮激素受体）表达增加，提示参与LC-PUFA生物合成调控。这些结果表明，在高盐度条件下培养杜氏假凤尾藻可以提高其n-3 LC-PUFA含量。同样，被认为是水产养殖饲料可持续替代品的其他多毛类的营养价值也可以通过盐度调节得到改善。

{"title":"Effects of salinity on the regulation of long-chain polyunsaturated fatty acid biosynthesis in the polychaete Platynereis dumerilii","authors":"Khalida Bainour, Óscar Monroig, Marc Ramos-Llorens, Juan C. Navarro","doi":"10.1016/j.cbpb.2025.111164","DOIUrl":"10.1016/j.cbpb.2025.111164","url":null,"abstract":"<div><div>Polychaetes are promising sources of n-3 long-chain (≥C<sub>20</sub>) polyunsaturated fatty acid (LC-PUFA) due to their ability to synthesise these essential nutrients through elongases, front-end desaturases (Fed), and methyl-end desaturases. Salinity can influence lipid metabolism in aquatic invertebrates by triggering osmotic stress that alters membrane lipid composition. This study investigates the effect of salinity on LC-PUFA biosynthesis in the polychaete, <em>Platynereis dumerilii</em>, a model organism in Evolutionary and Developmental Biology. In vivo trials were conducted with juveniles under standard conditions (18 °C, 16 L:8D photoperiod, frozen spinach as feed), evaluating three salinity levels: 30 ‰, 35 ‰, and 40 ‰. After one month, survival and growth were assessed, and samples were collected to evaluate the modulation of n-3 LC-PUFA biosynthesis by analysing total lipids, fatty acid (FA) profiles, and gene expression of elongases, desaturases, and transcription factors (TF). Despite a uniform diet, <em>P. dumerilii</em> at 40 ‰ salinity exhibited elevated levels of n-3 LC-PUFA (20:4n-3, 20:5n-3, 22:5n-3). Variations in FA profiles suggest salinity plays a regulatory role in lipid metabolism. Gene expression analysis showed significant upregulation of a Fed, with Δ6/Δ8 activity, under high salinity (40 ‰), consistent with reduced levels of its substrates, eicosadienoic acid (20:2n-6) and eicosatrienoic acid (20:3n-3). Additionally, the TF EcR (Ecdysone receptor) showed increased expression, suggesting involvement in LC-PUFA biosynthesis regulation. These findings indicate that cultivating <em>P. dumerilii</em> in high salinity may enhance its n-3 LC-PUFA content. Similarly, the nutritional value of other polychaetes, considered sustainable alternatives for aquaculture feed, may be improved through salinity modulation.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"281 ","pages":"Article 111164"},"PeriodicalIF":1.8,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145066315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptional and epigenetic responses to acute hypoxia exposure in rainbow trout (Oncorhynchus mykiss) 虹鳟（Oncorhynchus mykiss）急性缺氧暴露的转录和表观遗传反应。

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-09-12 DOI: 10.1016/j.cbpb.2025.111163

William Johnston, Sally Adil, Jan A. Mennigen

Rainbow trout, which are comparatively intolerant to low oxygen, face increasing hypoxic events. This study investigated their metabolic and molecular responses to acute hypoxia and investigated whether O₂-dependent epigenetic marks mediate these responses. Adult rainbow trout were exposed to normoxia (10.5 mg/L dissolved oxygen (DO)), 50 % hypoxia (5.25 mg/L DO) and 25 % oxygen saturation (2.61 mg/L DO) for 4 h. Plasma metabolites and cortisol were measured, as were transcripts of hypoxia-responsive genes involved in oxygen sensing, glucose/lactate metabolism, and lipid metabolism in liver, white/red muscle, and adipose tissue. Global DNA methylation and promoter-specific histone modifications (H3K4me3) were profiled in the liver. Lactate and, to a lesser extent, glucose concentrations increased, while lipid metabolites remained unchanged. The stress hormone cortisol was significantly elevated. At the tissue level, the liver was most sensitive to hypoxia and exhibited the most pronounced transcriptional changes. Hepatic gene transcripts involved in lactate transport and glycolysis increased at 50 % oxygen saturation, while gene transcripts involved in gluconeogenesis and fatty acid import and synthesis were induced at 25 % oxygen saturation. Hepatic global DNA methylation remained unchanged, but promoter-specific H3K4me3 modifications were observed for the phosphoenolpyruvate carboxykinase 1 gene. However, no direct correlation between pck1 H3K4me3 promoter occupancy and pck1 expression was evident, suggesting other transcriptional control mechanisms. This research provides insights into the tissue-specific metabolic responses and epigenetic mechanisms in rainbow trout under acute hypoxia, paving the way for comparative studies probing the role of oxygen-sensitive epigenetic markers in transcriptional responses to hypoxia in other fish species.

虹鳟鱼相对不耐低氧，面临越来越多的缺氧事件。本研究研究了它们对急性缺氧的代谢和分子反应，并研究了o2依赖性表观遗传标记是否介导了这些反应。成年虹鳟鱼分别暴露于常氧（溶解氧10.5 mg/L）、50% %缺氧（溶解氧5.25 mg/L）和25% %氧饱和度（溶解氧2.61 mg/L）环境4 h。测量血浆代谢物和皮质醇，以及参与氧感应、葡萄糖/乳酸代谢和肝脏、白/红肌肉和脂肪组织脂质代谢的缺氧反应基因的转录本。在肝脏中分析了全局DNA甲基化和启动子特异性组蛋白修饰（H3K4me3）。乳酸和葡萄糖浓度（在较小程度上）增加，而脂质代谢物保持不变。应激激素皮质醇显著升高。在组织水平上，肝脏对缺氧最敏感，并表现出最明显的转录变化。参与乳酸转运和糖酵解的肝脏基因转录本在50% %氧饱和度下增加，而参与糖异生和脂肪酸输入和合成的基因转录本在25% %氧饱和度下被诱导。肝脏整体DNA甲基化保持不变，但在磷酸烯醇丙酮酸羧激酶1基因上观察到启动子特异性的H3K4me3修饰。然而，pck1 H3K4me3启动子占用与pck1表达之间没有明显的直接相关性，提示存在其他转录调控机制。本研究揭示了虹鳟鱼在急性缺氧条件下的组织特异性代谢反应和表观遗传机制，为探讨氧敏感表观遗传标记在其他鱼类缺氧转录反应中的作用铺平了道路。

{"title":"Transcriptional and epigenetic responses to acute hypoxia exposure in rainbow trout (Oncorhynchus mykiss)","authors":"William Johnston, Sally Adil, Jan A. Mennigen","doi":"10.1016/j.cbpb.2025.111163","DOIUrl":"10.1016/j.cbpb.2025.111163","url":null,"abstract":"<div><div>Rainbow trout, which are comparatively intolerant to low oxygen, face increasing hypoxic events. This study investigated their metabolic and molecular responses to acute hypoxia and investigated whether O<sub>2</sub>-dependent epigenetic marks mediate these responses. Adult rainbow trout were exposed to normoxia (10.5 mg/L dissolved oxygen (DO)), 50 % hypoxia (5.25 mg/L DO) and 25 % oxygen saturation (2.61 mg/L DO) for 4 h. Plasma metabolites and cortisol were measured, as were transcripts of hypoxia-responsive genes involved in oxygen sensing, glucose/lactate metabolism, and lipid metabolism in liver, white/red muscle, and adipose tissue. Global DNA methylation and promoter-specific histone modifications (H3K4me3) were profiled in the liver. Lactate and, to a lesser extent, glucose concentrations increased, while lipid metabolites remained unchanged. The stress hormone cortisol was significantly elevated. At the tissue level, the liver was most sensitive to hypoxia and exhibited the most pronounced transcriptional changes. Hepatic gene transcripts involved in lactate transport and glycolysis increased at 50 % oxygen saturation, while gene transcripts involved in gluconeogenesis and fatty acid import and synthesis were induced at 25 % oxygen saturation. Hepatic global DNA methylation remained unchanged, but promoter-specific H3K4me3 modifications were observed for the phosphoenolpyruvate carboxykinase 1 gene. However, no direct correlation between <em>pck1</em> H3K4me3 promoter occupancy and <em>pck1</em> expression was evident, suggesting other transcriptional control mechanisms. This research provides insights into the tissue-specific metabolic responses and epigenetic mechanisms in rainbow trout under acute hypoxia, paving the way for comparative studies probing the role of oxygen-sensitive epigenetic markers in transcriptional responses to hypoxia in other fish species.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"281 ","pages":"Article 111163"},"PeriodicalIF":1.8,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145066293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary thiamine supplementation enhances the growth performance, digestive enzyme activity, intestine development, immunity and anti-inflammatory gene expression of juvenile olive flounder (Paralichthys olivaceus) 饲粮中添加硫胺素可提高橄榄比目鱼幼鱼的生长性能、消化酶活性、肠道发育、免疫力和抗炎基因表达。

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-09-10 DOI: 10.1016/j.cbpb.2025.111162

Yeonji Lee , Suhyeok Kim , Mirasha Hasanthi , Sanghyun Song , Sua Kim , Kyeong-Jun Lee

This research aimed to determine the dietary requirement of thiamine and evaluate its effects on olive flounder (Paralichthys olivaceus). Control diet (Con) was formulated without thiamine supplementation, while four additional diets were formulated with thiamine concentrations of 4, 8, 12 and 16 mg/kg diet (designated as T4, T8, T12 and T16, respectively). A total of 300 fish (initial weight, 55.5 ± 1.3 g) were randomly distributed into 15 tanks (triplicate groups per treatment, 20 fish per tank) and fed for 10 weeks. One-way ANOVA was performed to analyze treatment effects. Final body weight was significantly increased in all the thiamine-supplemented groups compared to the Con group. Significant improvements in weight gain, specific growth rate and feed utilization were observed in T4 and T16 groups compared to the Con group. Immune responses, including anti-protease, lysozyme and myeloperoxidase activities and total immnunoglobulin concentration were significantly increased in the T4-T12 thiamine-supplemented groups. Antioxidant activity was significantly increased in all thiamine-supplemented groups. Trypsin activity was significantly elevated in the 4–12 mg/kg groups, while amylase and lipase activities were enhanced in all thiamine-supplemented groups. Anti-inflammatory genes were significantly upregulated in thiamine-supplemented groups compared to the Con. Thiamine supplementation promoted an increase in goblet cell count and villi length in intestine. A broken-line regression analysis for weight gain indicated that the optimal thiamine requirement for olive flounder would be 3.05 mg/kg diet. These findings demonstrate the positive effects of dietary thiamine on growth, immunity, antioxidant activity, digestive enzyme activity and intestinal health in olive flounder.

本研究旨在确定饲料中硫胺素的需要量，并评价硫胺素对橄榄比目鱼的影响。对照组饲喂不添加硫胺素的饲粮（Con），添加硫胺素浓度为4、8、12和16 mg/kg的饲粮（分别命名为T4、T8、T12和T16）。试验选取初始体重为55.5 ± 1.3 g的300尾鱼，随机分为15个试验池（每个处理3个重复组，每个试验池20尾鱼），饲喂10 周。采用单因素方差分析分析治疗效果。与对照组相比，所有补充硫胺素组的最终体重均显著增加。与Con组相比，T4组和T16组的增重、特定生长率和饲料利用率均有显著提高。T4-T12硫胺素补充组的免疫应答，包括抗蛋白酶、溶菌酶和髓过氧化物酶活性以及总免疫球蛋白浓度显著升高。各硫胺素添加组抗氧化活性均显著提高。4 ~ 12 mg/kg组胰蛋白酶活性显著升高，各硫胺素添加组淀粉酶和脂肪酶活性均显著升高。与对照组相比，补充硫胺素组抗炎基因显著上调。补充硫胺素促进了肠杯状细胞计数和绒毛长度的增加。对增重的折线回归分析表明，橄榄比目鱼的最佳硫胺素需取量为3.05 mg/kg日粮。由此可见，饲粮中添加硫胺素对橄榄比目鱼的生长、免疫、抗氧化活性、消化酶活性和肠道健康均有积极影响。

{"title":"Dietary thiamine supplementation enhances the growth performance, digestive enzyme activity, intestine development, immunity and anti-inflammatory gene expression of juvenile olive flounder (Paralichthys olivaceus)","authors":"Yeonji Lee , Suhyeok Kim , Mirasha Hasanthi , Sanghyun Song , Sua Kim , Kyeong-Jun Lee","doi":"10.1016/j.cbpb.2025.111162","DOIUrl":"10.1016/j.cbpb.2025.111162","url":null,"abstract":"<div><div>This research aimed to determine the dietary requirement of thiamine and evaluate its effects on olive flounder (<em>Paralichthys olivaceus</em>). Control diet (Con) was formulated without thiamine supplementation, while four additional diets were formulated with thiamine concentrations of 4, 8, 12 and 16 mg/kg diet (designated as T4, T8, T12 and T16, respectively). A total of 300 fish (initial weight, 55.5 ± 1.3 g) were randomly distributed into 15 tanks (triplicate groups per treatment, 20 fish per tank) and fed for 10 weeks. One-way ANOVA was performed to analyze treatment effects. Final body weight was significantly increased in all the thiamine-supplemented groups compared to the Con group. Significant improvements in weight gain, specific growth rate and feed utilization were observed in T4 and T16 groups compared to the Con group. Immune responses, including anti-protease, lysozyme and myeloperoxidase activities and total immnunoglobulin concentration were significantly increased in the T4-T12 thiamine-supplemented groups. Antioxidant activity was significantly increased in all thiamine-supplemented groups. Trypsin activity was significantly elevated in the 4–12 mg/kg groups, while amylase and lipase activities were enhanced in all thiamine-supplemented groups. Anti-inflammatory genes were significantly upregulated in thiamine-supplemented groups compared to the Con. Thiamine supplementation promoted an increase in goblet cell count and villi length in intestine. A broken-line regression analysis for weight gain indicated that the optimal thiamine requirement for olive flounder would be 3.05 mg/kg diet. These findings demonstrate the positive effects of dietary thiamine on growth, immunity, antioxidant activity, digestive enzyme activity and intestinal health in olive flounder.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"281 ","pages":"Article 111162"},"PeriodicalIF":1.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145056083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth performance, lipid metabolism, gut histoarchitecture and immune and antioxidant related gene expression in juvenile Asian sea bass, Lates calcarifer fed peroxidized lipids with or without dietary selenium nanoparticles 饲料中添加或不添加纳米硒的过氧化物脂类对亚洲黑鲈幼鱼生长性能、脂质代谢、肠道组织结构以及免疫和抗氧化相关基因表达的影响。

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-09-08 DOI: 10.1016/j.cbpb.2025.111161

Asieh Najafi , Amir Parviz Salati , Sara Ferrando , Ali Shahriari , Mansour Torfi Mozanzadeh

This study evaluated the effects of dietary recovered frying soybean oil (RFSBO) and selenium nanoparticles (SeNPs) on growth performance, hepatic metabolism, intestinal morphology, and the expression of antioxidant, immune, and growth-related genes in juvenile Asian sea bass (Lates calcarifer, 41.5 ± 0.1 g) reared under high temperature (32–33 °C) and high salinity (38–40 ppt). Six diets were formulated: fresh soybean oil (FSBO), FSBO + SN (4 mg/kg SeNPs), 50 % RFSBO, 50 % RFSBO + SN, 100 % RFSBO, and 100 % RFSBO + SN. Fish (n = 450) were randomly assigned to 18 tanks and fed to apparent satiation three times daily for eight weeks. Fish fed 50 % RFSBO + SN achieved similar final weights to the FSBO group but with significantly better feed conversion ratio, improved gut wall, epithelial, and villus height, and lower malic enzyme activity, indicating reduced metabolic stress. Hepatic triglycerides were significantly lower in this group than in FSBO-fed fish, while glycogen content was maintained. In contrast, 100 % RFSBO caused histological damage, oxidative stress, elevated isocitrate dehydrogenase activity, and lipid imbalance, with SeNPs offering only partial mitigation. SeNP supplementation upregulated gpx1, lyz, il-1β, and igf1 expression under moderate oxidative stress but had limited effects under severe conditions. Overall, RFSBO can replace up to 50 % of dietary FSBO without compromising growth or intestinal health when combined with SeNPs, but higher levels reduce SeNP efficacy. These findings support the use of moderate RFSBO inclusion with SeNP supplementation to sustain fish health and performance under challenging environmental conditions.

本研究评估了饲料中添加回收油炸大豆油（RFSBO）和纳米硒（SeNPs）对高温（32-33 °C）和高盐度（38-40 ppt）饲养的亚洲海鲈鱼幼鱼（Lates calcarifer, 41.5 ± 0.1 g）生长性能、肝脏代谢、肠道形态以及抗氧化、免疫和生长相关基因表达的影响。配制6种饲粮：新鲜大豆油（FSBO）、FSBO + SN（4 mg/kg SeNPs）、50 % RFSBO、50 % RFSBO + SN、100 % RFSBO和100 % RFSBO + SN。鱼（n = 450）被随机分配到18个鱼缸中，每天三次喂饱，持续八周。饲喂50% % RFSBO + SN的鱼最终体重与FSBO组相近，但饲料系数显著提高，肠壁、上皮和绒毛高度提高，苹果酶活性降低，表明代谢应激降低。肝脏甘油三酯显著低于fsbo饲料组，同时糖原含量保持不变。相比之下，100 % RFSBO引起组织损伤、氧化应激、异柠檬酸脱氢酶活性升高和脂质失衡，而SeNPs仅能部分缓解。在中等氧化应激条件下，补充SeNP可上调gpx1、lyz、il-1β和igf1的表达，但在严重氧化应激条件下影响有限。总的来说，当与SeNPs联合使用时，RFSBO可以替代高达50% %的膳食FSBO，而不会影响生长或肠道健康，但较高的水平会降低SeNP的功效。这些发现支持在具有挑战性的环境条件下使用适度的RFSBO和SeNP补充剂来维持鱼类的健康和性能。

{"title":"Growth performance, lipid metabolism, gut histoarchitecture and immune and antioxidant related gene expression in juvenile Asian sea bass, Lates calcarifer fed peroxidized lipids with or without dietary selenium nanoparticles","authors":"Asieh Najafi , Amir Parviz Salati , Sara Ferrando , Ali Shahriari , Mansour Torfi Mozanzadeh","doi":"10.1016/j.cbpb.2025.111161","DOIUrl":"10.1016/j.cbpb.2025.111161","url":null,"abstract":"<div><div>This study evaluated the effects of dietary recovered frying soybean oil (RFSBO) and selenium nanoparticles (SeNPs) on growth performance, hepatic metabolism, intestinal morphology, and the expression of antioxidant, immune, and growth-related genes in juvenile Asian sea bass (<em>Lates calcarifer</em>, 41.5 ± 0.1 g) reared under high temperature (32–33 °C) and high salinity (38–40 ppt). Six diets were formulated: fresh soybean oil (FSBO), FSBO + SN (4 mg/kg SeNPs), 50 % RFSBO, 50 % RFSBO + SN, 100 % RFSBO, and 100 % RFSBO + SN. Fish (<em>n</em> = 450) were randomly assigned to 18 tanks and fed to apparent satiation three times daily for eight weeks. Fish fed 50 % RFSBO + SN achieved similar final weights to the FSBO group but with significantly better feed conversion ratio, improved gut wall, epithelial, and villus height, and lower malic enzyme activity, indicating reduced metabolic stress. Hepatic triglycerides were significantly lower in this group than in FSBO-fed fish, while glycogen content was maintained. In contrast, 100 % RFSBO caused histological damage, oxidative stress, elevated isocitrate dehydrogenase activity, and lipid imbalance, with SeNPs offering only partial mitigation. SeNP supplementation upregulated <em>gpx1</em>, <em>lyz</em>, <em>il-1β</em>, and <em>igf1</em> expression under moderate oxidative stress but had limited effects under severe conditions. Overall, RFSBO can replace up to 50 % of dietary FSBO without compromising growth or intestinal health when combined with SeNPs, but higher levels reduce SeNP efficacy. These findings support the use of moderate RFSBO inclusion with SeNP supplementation to sustain fish health and performance under challenging environmental conditions.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"281 ","pages":"Article 111161"},"PeriodicalIF":1.8,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and purification of lipovitellin-like egg yolk protein in a stony coral, Acropora aff. tenuis 石珊瑚（Acropora af . tenuis）卵黄脂样蛋白的鉴定与纯化

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-08-30 DOI: 10.1016/j.cbpb.2025.111152

Haruna Amano , Juna Kamoshita , Masami Ishimoto , Minami Arai , Mitsuru Jimbo , Ko Yasumoto , Go Suzuki

Three egg proteins (EP1, EP2, and EP3) were detected in ovulated eggs of Acropora aff. tenuis, a reef-building stony coral found in tropical and subtropical areas. The proteins were separated into different fractions by gel filtration chromatography, and different patterns were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting with antiserum against A. aff. tenuis egg extract (anti-AtE). EP2 was purified from ovulated egg extracts using a combination of hydroxylapatite and gel filtration chromatography. The molecular mass of native EP2 was estimated to be 286 kDa. SDS-PAGE revealed bands at 70.5, 67.7, 49.6, and 22.9 kDa, with the 70.5 and 67.7 kDa bands staining positive for lipid (Sudan black B). These results indicate that purified EP2 is a lipoprotein in A. aff. tenuis eggs, resembling lipovitellin, the major yolk protein derived from the egg yolk precursor, vitellogenin (Vtg), in oviparous vertebrates. Furthermore, EP2 and its bands were digested with trypsin and analyzed by liquid chromatography using a quadrupole time-of-flight tandem mass spectrometer (LC-QTOF-MS). All fragments were identified as A. aff. tenuis Vtg. This is the first report of the purification and characterization of a lipovitellin-like egg yolk protein in non-bilaterian oviparous animals.

在热带和亚热带造礁石珊瑚（Acropora afft . tenuis）的卵细胞中检测到3种卵细胞蛋白（EP1、EP2和EP3）。采用凝胶过滤层析法将蛋白分离成不同的组分，并采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和抗黄颡鱼蛋提取物血清（anti-AtE）进行免疫印迹（Western blotting）检测。用羟基磷灰石和凝胶过滤层析相结合的方法从卵泡提取物中纯化EP2。原生EP2的分子质量估计为286 kDa。SDS-PAGE显示70.5,67.7,49.6和22.9 kDa的条带，其中70.5和67.7 kDa的条带染色为脂质阳性（苏丹黑B）。这些结果表明，纯化后的EP2是黄颡鱼卵中的一种脂蛋白，类似于卵黄蛋白（lipovitellin），后者是卵黄前体卵黄原蛋白（vitellogenin, Vtg）在卵生脊椎动物中的主要卵黄蛋白。用胰蛋白酶消化EP2及其谱带，用四极杆飞行时间串联质谱（LC-QTOF-MS）进行液相色谱分析。所有片段鉴定为A. aff. tenuis Vtg。本文首次报道了非双侧卵生动物卵黄蛋白的纯化和特性。

{"title":"Characterization and purification of lipovitellin-like egg yolk protein in a stony coral, Acropora aff. tenuis","authors":"Haruna Amano , Juna Kamoshita , Masami Ishimoto , Minami Arai , Mitsuru Jimbo , Ko Yasumoto , Go Suzuki","doi":"10.1016/j.cbpb.2025.111152","DOIUrl":"10.1016/j.cbpb.2025.111152","url":null,"abstract":"<div><div>Three egg proteins (EP1, EP2, and EP3) were detected in ovulated eggs of <em>Acropora</em> aff. <em>tenuis</em>, a reef-building stony coral found in tropical and subtropical areas. The proteins were separated into different fractions by gel filtration chromatography, and different patterns were observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting with antiserum against <em>A.</em> aff. <em>tenuis</em> egg extract (anti-AtE). EP2 was purified from ovulated egg extracts using a combination of hydroxylapatite and gel filtration chromatography. The molecular mass of native EP2 was estimated to be 286 kDa. SDS-PAGE revealed bands at 70.5, 67.7, 49.6, and 22.9 kDa, with the 70.5 and 67.7 kDa bands staining positive for lipid (Sudan black B). These results indicate that purified EP2 is a lipoprotein in <em>A.</em> aff. <em>tenuis</em> eggs, resembling lipovitellin, the major yolk protein derived from the egg yolk precursor, vitellogenin (Vtg), in oviparous vertebrates. Furthermore, EP2 and its bands were digested with trypsin and analyzed by liquid chromatography using a quadrupole time-of-flight tandem mass spectrometer (LC-QTOF-MS). All fragments were identified as <em>A.</em> aff. <em>tenuis</em> Vtg. This is the first report of the purification and characterization of a lipovitellin-like egg yolk protein in non-bilaterian oviparous animals.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"280 ","pages":"Article 111152"},"PeriodicalIF":1.8,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144921425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chitosan nanogel mitigates Shewanella-induced oxidative stress, brain neurotransmitter imbalance, biochemical, hepatic, and renal dysfunction, and histopathological changes in Nile tilapia 壳聚糖纳米凝胶可减轻尼罗罗非鱼shewanilla诱导的氧化应激、脑神经递质失衡、生化、肝肾功能障碍和组织病理学变化

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-08-29 DOI: 10.1016/j.cbpb.2025.111151

Rowida E. Ibrahim , Gehad E. Elshopakey , Ahmed S. AlSaqufi , Abdallah Tageldein Mansour , Yousef Alkhamis , Hesham A. Hassanien , Ahmed Abbas , Sameh H. Ismail , Tarek Khamis , Afaf N. Abdel Rahman

This study investigated the potential for chitosan nanogel (CNG) to mitigate adverse consequences of infection by Shewanella spp. in the Nile tilapia, Oreochromis niloticus. A total of 160 fish (average weight = 27.55 ± 1.50 g) were allocated to four treatments, each with four replicates, for 14 days. Fish in the control and CNG only groups were intraperitoneally injected with 0.2 mL of sterile saline and placed in water treated with 0 or 75 μg/L, respectively, of CNG. Fish in the Shewanella and CNG + Shewanella groups were injected with 0.2 mL of Shewanella spp. (0.14 × 10⁵ colony forming units) and placed in water treated with 0 or 75 μg/L, respectively, of CNG. Infection by Shewanella induced hemorrhages on the skin and base of the fins, fin rot, and scale loss. Shewanella infection decreased the activity of glutathione peroxidase and catalase, with increased malondialdehyde concentration. Shewanella decreased the brain neurotransmitters (dopamine and serotonin), total protein, and globulin levels, while it increased serum glucose and cortisol. The hepatic and renal dysfunction indicators alanine aminotransferase, aspartate aminotransferase, and creatinine were significantly increased by Shewanella infection. Histopathological changes were produced in the liver, kidney, gills, and brain of Shewanella-infected fish. Notably, Shewanella-infected fish in CNG-treated water had reduced abnormal signs, stress markers, and hepatic and renal dysfunction indicators. CNG water application elevated the brain levels of neurotransmitters and protein and improved the tissue histology. Overall, CNG as a water application is useful for mitigating the adverse effects of Shewanella spp. in Nile tilapia.

本研究探讨了壳聚糖纳米凝胶（CNG）在尼罗罗非鱼（Oreochromis niloticus）体内减轻希瓦氏菌感染不良后果的潜力。试验选用平均体重为27.55±1.50 g的160尾鱼，分为4个处理，每个处理4个重复，试验期14 d。对照组和纯CNG组分别腹腔注射无菌生理盐水0.2 mL，置于CNG浓度为0或75 μg/L的水中。Shewanella组和CNG + Shewanella组分别注射0.2 mL Shewanella spp. （0.14 × 105集落形成单位），置于CNG浓度为0或75 μg/L的水中。被希瓦氏菌感染后，皮肤和鱼鳍底部出血，鱼鳍腐烂，鱼鳞脱落。随着丙二醛浓度的升高，希瓦氏菌感染降低了谷胱甘肽过氧化物酶和过氧化氢酶的活性。希瓦氏菌降低了大脑神经递质（多巴胺和血清素）、总蛋白和球蛋白水平，同时增加了血清葡萄糖和皮质醇。感染希瓦氏菌后，肝肾功能指标丙氨酸转氨酶、天冬氨酸转氨酶、肌酐显著升高。感染雪瓦氏菌的鱼的肝脏、肾脏、鳃和大脑均发生组织病理学改变。值得注意的是，在经过天然气处理的水中，感染了雪瓦氏菌的鱼的异常体征、应激标志物以及肝肾功能障碍指标都有所减少。CNG水提高了脑内神经递质和蛋白质水平，改善了脑组织组织学。总的来说，天然气作为一种水应用是有用的，以减轻希瓦氏菌对尼罗罗非鱼的不利影响。

{"title":"Chitosan nanogel mitigates Shewanella-induced oxidative stress, brain neurotransmitter imbalance, biochemical, hepatic, and renal dysfunction, and histopathological changes in Nile tilapia","authors":"Rowida E. Ibrahim , Gehad E. Elshopakey , Ahmed S. AlSaqufi , Abdallah Tageldein Mansour , Yousef Alkhamis , Hesham A. Hassanien , Ahmed Abbas , Sameh H. Ismail , Tarek Khamis , Afaf N. Abdel Rahman","doi":"10.1016/j.cbpb.2025.111151","DOIUrl":"10.1016/j.cbpb.2025.111151","url":null,"abstract":"<div><div>This study investigated the potential for chitosan nanogel (CNG) to mitigate adverse consequences of infection by <em>Shewanella</em> spp. in the Nile tilapia, <em>Oreochromis niloticus</em>. A total of 160 fish (average weight = 27.55 ± 1.50 g) were allocated to four treatments, each with four replicates, for 14 days. Fish in the control and CNG only groups were intraperitoneally injected with 0.2 mL of sterile saline and placed in water treated with 0 or 75 μg/L, respectively, of CNG. Fish in the <em>Shewanella</em> and CNG + <em>Shewanella</em> groups were injected with 0.2 mL of <em>Shewanella</em> spp. (0.14 × 10<sup>5</sup> colony forming units) and placed in water treated with 0 or 75 μg/L, respectively, of CNG. Infection by <em>Shewanella</em> induced hemorrhages on the skin and base of the fins, fin rot, and scale loss. <em>Shewanella</em> infection decreased the activity of glutathione peroxidase and catalase, with increased malondialdehyde concentration. <em>Shewanella</em> decreased the brain neurotransmitters (dopamine and serotonin), total protein, and globulin levels, while it increased serum glucose and cortisol. The hepatic and renal dysfunction indicators alanine aminotransferase, aspartate aminotransferase, and creatinine were significantly increased by <em>Shewanella</em> infection. Histopathological changes were produced in the liver, kidney, gills, and brain of <em>Shewanella</em>-infected fish. Notably, <em>Shewanella</em>-infected fish in CNG-treated water had reduced abnormal signs, stress markers, and hepatic and renal dysfunction indicators. CNG water application elevated the brain levels of neurotransmitters and protein and improved the tissue histology. Overall, CNG as a water application is useful for mitigating the adverse effects of <em>Shewanella</em> spp. in Nile tilapia.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"280 ","pages":"Article 111151"},"PeriodicalIF":1.8,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144921578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated yeast one-hybrid and molecular docking reveal the binding specificity of FTZ-F1 to GnRHR and Dmrta2 promoters in the scallop, Chlamys farreri 酵母单杂交和分子对接揭示了FTZ-F1在栉孔扇贝中与GnRHR和Dmrta2启动子的结合特异性

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-08-23 DOI: 10.1016/j.cbpb.2025.111149

Hui Fu, Qichao Fan, Jingjing Miao, Ya Liu, Yuxin Wen, Luqing Pan

The scallop (Chlamys farreri) is an important aquaculture species, and understanding its reproductive regulation is a major research focus. Previous studies have suggested that the orphan nuclear receptor fushi tarazu factor 1 (FTZF1), a member of the nuclear receptor subfamily 5 group A (NR5A), may play a role in regulating gonadal development and differentiation in scallops. In this study, we demonstrate through yeast one-hybrid assays that FTZ-F1 directly binds to the promoters of the gonadotropin releasing hormone receptor (GnRHR), which is involved in steroidogenesis, and DMRT-like family A2 (Dmrta2), which is involved in sex determination. Molecular docking and sequence alignment identified 11 key amino acids within the DNA-binding domain （DBD） essential for promoter binding, 10 of which resided in the conserved DBD region. These findings provide molecular insights into reproductive regulation in bivalves and establish a foundation for further research on sex differentiation and steroidogenesis.

扇贝（Chlamys farreri）是一种重要的水产养殖物种，了解其繁殖调控是一个重要的研究热点。以往的研究表明，孤儿核受体fushi tarazu因子1 （FTZF1）作为核受体亚家族5 a组（NR5A）的成员，可能在扇贝的性腺发育和分化中起调节作用。在这项研究中，我们通过酵母单杂交实验证明，FTZ-F1直接结合促性腺激素释放激素受体（GnRHR）的启动子，该受体参与类固醇生成，dmrt样家族A2 （Dmrta2）参与性别决定。分子对接和序列比对鉴定了启动子结合必需的dna结合域（DBD）内的11个关键氨基酸，其中10个位于保守的DBD区域。这些发现为双壳类动物的生殖调控提供了分子视角，为进一步研究双壳类动物的性别分化和甾体发生奠定了基础。

{"title":"Integrated yeast one-hybrid and molecular docking reveal the binding specificity of FTZ-F1 to GnRHR and Dmrta2 promoters in the scallop, Chlamys farreri","authors":"Hui Fu, Qichao Fan, Jingjing Miao, Ya Liu, Yuxin Wen, Luqing Pan","doi":"10.1016/j.cbpb.2025.111149","DOIUrl":"10.1016/j.cbpb.2025.111149","url":null,"abstract":"<div><div>The scallop (<em>Chlamys farreri</em>) is an important aquaculture species, and understanding its reproductive regulation is a major research focus. Previous studies have suggested that the orphan nuclear receptor fushi tarazu factor 1 (FTZ<img>F1), a member of the nuclear receptor subfamily 5 group A (NR5A), may play a role in regulating gonadal development and differentiation in scallops. In this study, we demonstrate through yeast one-hybrid assays that FTZ-F1 directly binds to the promoters of the gonadotropin releasing hormone receptor (<em>GnRHR</em>), which is involved in steroidogenesis, and DMRT-like family A2 (<em>Dmrta2</em>), which is involved in sex determination. Molecular docking and sequence alignment identified 11 key amino acids within the DNA-binding domain （DBD） essential for promoter binding, 10 of which resided in the conserved DBD region. These findings provide molecular insights into reproductive regulation in bivalves and establish a foundation for further research on sex differentiation and steroidogenesis.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"280 ","pages":"Article 111149"},"PeriodicalIF":1.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual roles of reactive oxygen species in ascidian brain regeneration and degeneration 活性氧在海鞘脑再生和退化中的双重作用

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-08-23 DOI: 10.1016/j.cbpb.2025.111150

Thuany da Silva Nogueira , Andressa de Abreu Mello , Nathany da Silva Nogueira , Cintia Monteiro-de-Barros

Reactive oxygen species (ROS) are metabolically generated by neural cells and are essential for maintaining cellular homeostasis. Due to the high metabolic activity and oxygen consumption of the nervous system, large amounts of ROS are produced. Although ROS are traditionally associated with oxidative stress and degeneration, recent studies suggest that they may also play a beneficial role in neural repair. To explore the role of ROS in central nervous system (CNS) regeneration, we employed the adult ascidian Styela plicata, which can regenerate its entire brain within 10 days, as a model. We aimed to elucidate the functions of ROS and the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) in the formation of synapses and/or cell proliferation in the CNS of the ascidian S. plicata following the systemic injection of the neurotoxin 3-acetylpyridine (3-AP). The data revealed two distinct periods of high ROS production: at 0.5 and 5 days. SOD and CAT activity increased only at 5 days. Lipid peroxidation was highest at 0.5 days and gradually decreased during the 10 day post-injection monitoring period. The proliferation marker Ki-67 and the synapse marker synaptophysin were elevated at 3 and 5 days, respectively. These findings suggest a dual role for ROS: an early phase linked to neurodegeneration and a later phase associated with regeneration. This highlights the complex role of redox signaling in CNS regeneration.

活性氧（ROS）是由神经细胞代谢产生的，对维持细胞稳态至关重要。由于神经系统的高代谢活动和氧气消耗，产生大量ROS。虽然传统上认为活性氧与氧化应激和变性有关，但最近的研究表明，它们也可能在神经修复中发挥有益作用。为了探讨活性氧在中枢神经系统（CNS）再生中的作用，我们以能在10天内再生全脑的成体海鞘（ascidian Styela plicata）为模型。我们旨在阐明ROS和抗氧化酶超氧化物歧化酶（SOD）和过氧化氢酶（CAT）在神经毒素3-乙酰吡啶（3-AP）全身注射后，在皱襞海鞘中枢神经系统突触形成和/或细胞增殖中的作用。数据显示两个不同的高ROS产生时期：0.5天和5天。SOD和CAT活性仅在第5天升高。脂质过氧化在0.5 d时最高，在注射后10 d监测期内逐渐降低。增殖标志物Ki-67和突触标志物synaptophysin分别在第3天和第5天升高。这些发现表明ROS具有双重作用：早期与神经退行性变有关，晚期与再生有关。这突出了氧化还原信号在中枢神经系统再生中的复杂作用。

{"title":"Dual roles of reactive oxygen species in ascidian brain regeneration and degeneration","authors":"Thuany da Silva Nogueira , Andressa de Abreu Mello , Nathany da Silva Nogueira , Cintia Monteiro-de-Barros","doi":"10.1016/j.cbpb.2025.111150","DOIUrl":"10.1016/j.cbpb.2025.111150","url":null,"abstract":"<div><div>Reactive oxygen species (ROS) are metabolically generated by neural cells and are essential for maintaining cellular homeostasis. Due to the high metabolic activity and oxygen consumption of the nervous system, large amounts of ROS are produced. Although ROS are traditionally associated with oxidative stress and degeneration, recent studies suggest that they may also play a beneficial role in neural repair. To explore the role of ROS in central nervous system (CNS) regeneration, we employed the adult ascidian <em>Styela plicata</em>, which can regenerate its entire brain within 10 days, as a model. We aimed to elucidate the functions of ROS and the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) in the formation of synapses and/or cell proliferation in the CNS of the ascidian <em>S. plicata</em> following the systemic injection of the neurotoxin 3-acetylpyridine (3-AP). The data revealed two distinct periods of high ROS production: at 0.5 and 5 days. SOD and CAT activity increased only at 5 days. Lipid peroxidation was highest at 0.5 days and gradually decreased during the 10 day post-injection monitoring period. The proliferation marker Ki-67 and the synapse marker synaptophysin were elevated at 3 and 5 days, respectively. These findings suggest a dual role for ROS: an early phase linked to neurodegeneration and a later phase associated with regeneration. This highlights the complex role of redox signaling in CNS regeneration.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"280 ","pages":"Article 111150"},"PeriodicalIF":1.8,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular expression of genes related to sex differentiation and their response to sex steroid hormones in the clam Chinese cyclina (Cyclina sinensis) 中华环蚌性别分化相关基因的分子表达及其对性类固醇激素的响应

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-08-21 DOI: 10.1016/j.cbpb.2025.111148

Mengge Xu , Siting Wang , Chen Liang , Dan Yang , Meimei Liu , Zhiguo Dong

This study investigated the tissue-specific expression and subcellular localization of sex differentiation-related genes of Cyclina sinensis, and then analyzed the effects of hormone treatment (1 and 50 μg/L estradiol treatment (E-1, E-50), 1 and 50 μg/L testosterone (T-1, T-50), and a control group (C)) on the sex ratio, gonadosomatic index, hepatosomatic index, hormone content, and expression of sex differentiation-related genes of juvenile C. sinensis. Vitellogenin (Vg), testis-specific serine kinase 1 (Tssk1), doublesex and mab-3 related transcription factor 1 (Dmrt1), and Wnt family member 4 (Wnt4) were significantly expressed in the gonads of adult C. sinensis, and the in situ hybridization signals of forkhead box L2 (Foxl2) and SRY-box transcription factor 9 (Sox9) were localized in nuclei of oogonia and oocytes and in spermatogonia, spermatocytes, and sperm, respectively. The male to female ratio was 7:3 in group T-1 after 60 days of hormone treatment, but this effect was not statistically significant. In the E-1 group, Vg was highly expressed in the ovary at days 30 and 120. TSSK1 was highly expressed in the testes of the T-1 and T-50 groups at 120 days (60 days after the cessation of hormone treatment). The expression of Foxl2 was significantly higher in the ovaries of the T-1 group at 60 days, relative to the control and other hormone-treated groups. Sox9 was highly expressed in the testes at 120 days. The concentrations of testosterone and estradiol in the gonad of C. sinensis increased initially and then decreased. Overall, these results revealed that hormones caused disruption of sex determination and expression of sex-differentiation genes in juvenile C. sinensis.

本研究研究了中华月子性别分化相关基因的组织特异性表达和亚细胞定位，分析了激素处理(1和50 μg/L雌二醇处理（E-1、E-50）、1和50 μg/L睾酮处理（T-1、T-50）和对照组(C)对中华月子幼鱼性别比、性腺指数、肝体指数、激素含量和性别分化相关基因表达的影响。卵黄原蛋白（Vg）、睾丸特异性丝氨酸激酶1 （Tssk1）、双性和单抗-3相关转录因子1 （Dmrt1）和Wnt家族成员4 （Wnt4）在成虫性腺中显著表达，叉头盒L2 （Foxl2）和SRY-box转录因子9 （Sox9）的原位杂交信号分别定位于卵原核和卵母细胞核以及精原细胞、精母细胞和精子中。激素治疗60 d后，T-1组男女比例为7:3，但差异无统计学意义。在E-1组中，Vg在30天和120天在卵巢中高表达。在T-1组和T-50组的睾丸中，TSSK1在120天（激素治疗停止后60天）高表达。与对照组和其他激素治疗组相比，T-1组卵巢中Foxl2的表达在60天时显著升高。第120天，Sox9在睾丸中高表达。雌、睾酮浓度呈先升高后降低的趋势。综上所述，这些结果表明激素干扰了中华绒螯蟹幼鱼性别决定和性别分化基因的表达。

{"title":"Molecular expression of genes related to sex differentiation and their response to sex steroid hormones in the clam Chinese cyclina (Cyclina sinensis)","authors":"Mengge Xu , Siting Wang , Chen Liang , Dan Yang , Meimei Liu , Zhiguo Dong","doi":"10.1016/j.cbpb.2025.111148","DOIUrl":"10.1016/j.cbpb.2025.111148","url":null,"abstract":"<div><div>This study investigated the tissue-specific expression and subcellular localization of sex differentiation-related genes of <em>Cyclina sinensis,</em> and then analyzed the effects of hormone treatment (1 and 50 μg/L estradiol treatment (E-1, E-50), 1 and 50 μg/L testosterone (T-1, T-50), and a control group (C)) on the sex ratio, gonadosomatic index, hepatosomatic index, hormone content, and expression of sex differentiation-related genes of juvenile <em>C. sinensis</em>. <em>Vitellogenin</em> (<em>Vg</em>), <em>testis-specific serine kinase 1</em> (<em>Tssk1</em>), <em>doublesex and mab-3 related transcription factor 1</em> (<em>Dmrt1</em>), and <em>Wnt</em> family member 4 (<em>Wnt4</em>) were significantly expressed in the gonads of adult <em>C. sinensis</em>, and the <em>in situ</em> hybridization signals of <em>forkhead box L2</em> (<em>Foxl2</em>) and <em>SRY-box transcription factor 9</em> (<em>Sox9</em>) were localized in nuclei of oogonia and oocytes and in spermatogonia, spermatocytes, and sperm, respectively. The male to female ratio was 7:3 in group T-1 after 60 days of hormone treatment, but this effect was not statistically significant. In the E-1 group, <em>Vg</em> was highly expressed in the ovary at days 30 and 120. <em>TSSK1</em> was highly expressed in the testes of the T-1 and T-50 groups at 120 days (60 days after the cessation of hormone treatment). The expression of <em>Foxl2</em> was significantly higher in the ovaries of the T-1 group at 60 days, relative to the control and other hormone-treated groups. <em>Sox9</em> was highly expressed in the testes at 120 days. The concentrations of testosterone and estradiol in the gonad of <em>C. sinensis</em> increased initially and then decreased. Overall, these results revealed that hormones caused disruption of sex determination and expression of sex-differentiation genes in juvenile <em>C. sinensis</em>.</div></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"280 ","pages":"Article 111148"},"PeriodicalIF":1.8,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144921582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas9-mediated editing of ptprfb (protein tyrosine phosphatase receptor type fb) reveals its regulatory role in zebrafish spermatogenesis CRISPR/ cas9介导的ptprfb（蛋白酪氨酸磷酸酶受体类型fb）编辑揭示了其在斑马鱼精子发生中的调节作用

IF 1.8 3区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology

Pub Date : 2025-08-18 DOI: 10.1016/j.cbpb.2025.111146

Xishuang Shan , Xiya Zhang , Binbin Tao , Yanlong Song , Zuoyan Zhu , Wei Hu , Ji Chen

Gonadal development and gamete maturation are essential for fish reproduction. The protein tyrosine phosphatase receptor type Fb (Ptprfb) is a member of the tyrosine phosphatase family. In the present study, we used CRISPR/Cas9 to mutate ptprfb in zebrafish. A significantly reduced natural fertilization rate of sperm from mutant fish was observed. The mutant fish produced fewer sperm with shorter flagella, and a smaller proportion of sperm could be activated. RNA-seq analysis revealed abnormal expression of some genes in testicular cells, such as hemoglobin subunit βA1 (hbba1), myosin heavy chain 11b (myh11b), and transgelin (tagln), as well as some genes involved in focal adhesion formation. These findings demonstrate that ptprfb contributes to spermatogenesis in zebrafish, and its dysfunction can adversely affect both the quantity and quality of sperm.

性腺发育和配子成熟对鱼类繁殖至关重要。蛋白酪氨酸磷酸酶受体类型Fb （Ptprfb）是酪氨酸磷酸酶家族的一员。在本研究中，我们使用CRISPR/Cas9对斑马鱼的ptprfb进行突变。观察到突变鱼精子的自然受精率显著降低。突变鱼产生的精子更少，鞭毛更短，精子被激活的比例更小。RNA-seq分析显示，睾丸细胞中血红蛋白亚基βA1 （hbba1）、肌球蛋白重链11b （myh11b）、转胶蛋白（tagln）等基因以及一些参与局灶黏附形成的基因表达异常。这些发现表明ptprfb参与了斑马鱼的精子发生，其功能障碍会对精子的数量和质量产生不利影响。

引用次数: 0