Arthropod Structure & Development最新文献

Tachinidae are one of the most diverse clades of Diptera. All tachinids are parasitoids of insects and other arthropods, and thus are considered an important source of biological pest control. Antennae are the most important olfactory organs of Tachinidae playing key roles in their lives, especially in locating hosts, and details of antennal ultrastructure could provide useful features for phylogenetic studies and understanding their adaptive evolution. Despite the ecological and evolutionary importance of antennae, the current knowledge of antennal ultrastructure is scarce for Tachinidae. Our study examined antennal sensilla of thirteen species belonging to thirteen genera within eleven tribes of all the four subfamilies (Phasiinae, Dexiinae, Tachininae, and Exoristinae): Beskia aelops Walker, Trichodura sp., Voria ruralis (Fallén), Zelia sp., Cylindromyia carinata Townsend, Phasia xenos Townsend, Neomintho sp., Genea australis (Townsend), Copecrypta sp., Hystricia sp., Belvosia sp., Leschenaultia sp., and Winthemia pinguis (Fabricius). Types, length and distribution of antennal sensilla were investigated via scanning electron microscopy (SEM). Our comparative analysis summarized 29 variable characters and we evaluated their phylogenetic signal for subfamilial, tribal and generic/specific levels, showing that antennal ultrastructure could be a reliable source of characters for phylogenetic analysis. Our findings demonstrate the remarkable diversity of the antennal ultrastructure of Tachinidae.

The structure and functional morphology of the mouthparts were investigated in adult hangingfly Bicaubittacus appendiculatus (Esben-Petersen, 1927) by scanning electron microscopy and histological serial sections. The mandibulate mouthparts consist of a labrum-epipharynx, paired mandibles and maxillae, and unpaired labium and hypopharynx. The labrum is elongated and tapered toward the apex. The epipharynx is furnished with numerous sensilla. The mandibles are sword-shaped, with an outer sharp tooth curved mesad and an inner blunt corner. The basal region of each mandible processes a conical projection. The maxillae are well-developed, each consisting of a sclerotized cardo, an elongated stipes, which bears an inner lacinia, an outer galea, and laterally a five-segmented maxillary palp. The labium is formed by a postmentum, a prementum and a pair of two-segmented labial palps. The hypopharynx is concave inward on the anterior side, with numerous microtrichia on the posterior surface. Seven types of sensilla were found on the mouthparts: sensilla basiconica on the epipharynx, and maxillary and labial palps; sensilla chaetica on the epipharynx; sensilla palmata, sensilla placoidea and sensilla trichodea on the epipharynx and maxillary palp; sensilla campaniformia and hair plates on the basal joints of palps. The sensillar function and the feeding mechanism of mouthparts in bittacids are briefly discussed.

The midgut tract of decapods is a digestive organ involved in the synthesis of peritrophic membrane, food transport, absorption of nutrients, and osmoregulation. The midgut tract has been described in detail in adult decapods, but little information is available regarding the morphology and ultrastructure of the midgut tract in larval stages. The present study describes the midgut tract and the midgut–hindgut junction of the larvae of the common spider crab Maja brachydactyla Balss, 1922 using techniques that included dissection, light microscopy, and electron microscopy. The study is mainly focused on the stages of zoea I and megalopa. The results obtained in this study show that the larval midgut tract is a short and simple tube positioned anteriorly, between the stomach and the hindgut tract. During larval development, the maximum length of the midgut tract increases significantly, but no differences were found on either the maximum diameter or the morphological traits of the organ. The midgut tract is active at least ca. 12 h after hatching, as suggested by the presence of the peritrophic membrane in the lumen, the presence of abundant electro-dense vesicles in the cell apex, and the release of the vesicle content on the organ lumen. The midgut–hindgut junction forms an abrupt transition between the midgut tract and the hindgut tract in which epithelial cells with mixed features of midgut and hindgut do not occur.

Holometabolan brains undergo structural and allometric changes and complex reorganizations during metamorphosis. In minute egg parasitoids, brain formation is shifted to the late larva and young pupa, due to extreme de-embryonization. The brains of Megaphragma wasps undergo denucleation, the details of which remained unknown. We describe the morphological and volumetric changes in the brain of Megaphragma viggianii (Trichogrammatidae) during pupal development with emphasis on the lysis of nuclei and show that the absolute and relative volume of the brain decrease by a factor of 5 from prepupa to adult at the expense of the cell body rind. The first foci of lysis appear during early pupal development, but most nuclei (up to 97%) are lost between pharate adult and adult. The first signs of lysis (destruction of the nuclear envelopes) occur in pupae with red eyes. The number of lysis foci (organelle destruction and increasing number of lysosomes and degree of chromatin compaction) strongly increases in pupae with black eyes. The cell body rind volume strongly decreases during pupal development (in larger insects it increases slightly or remains unchanged). Elucidation of the lysis of nuclei in neurons and of the functioning of an anucleate brain is an important objective for neuroscience.

The soldier caste of termites uses sensilla to sense pheromonal, tactile, and vibrational cues to communicate inside and outside their nest. Although sensilla with many modalities on the antennae of subterranean termites have been well explored, there remains a lack of information regarding sensillum characteristics and distribution of the nonolfactory organs of the soldier caste in the Coptotermes genus. In this study, the ultrastructure of sensilla from the soldier caste of three Coptotermes spp. (Coptotermes formosanus, Coptotermes curvignathus, and Coptotermes gestroi) was observed by scanning and transmission electron microscopy, and the putative function of each type was deduced. Six total sensillum types were observed, with two mechanoreceptive sensillum types (hair and plate). The long flexible-peg mechanoreceptive sensilla may work as contact-chemoreceptive sensilla due to their elongated dendritic outer segments and uniporous characteristics. There was a significant depletion of mechano-chemoreceptive sensillum numbers in C. gestroi, which was compensated by a high density of short-peg mechanoreceptive sensilla on the pronotum. Finally, cuticular and innervation characteristics of thermo-/hygrosensitive sensilla were observed for the first time on the labrum of the soldier caste of Coptotermes.

Distinct life stages may experience different selection pressures influencing phenotypic evolution. Morphological evolution is also constrained by early phenotypes, since early development forms the phenotypic basis of later development. This work investigates evolutionary-developmental modification in three life stages and both sexes of 24 Rhipicephalus species using phylogenetic comparative methods for geometric morphometrics of basis capituli (basal mouthpart structure used for host attachment), and scutum or conscutum areas (proxy for overall body size). Findings indicate species using large hosts at early life stages have distinct basis capituli shapes, correlated with host size, enabling attachment to the tough skins of large hosts. Host-truncate species (one- and two-host) generally retain these adaptive features into later life stages, suggesting neoteny is linked to the evolution of host truncation. In contrast, species using small hosts at early life stages have lost these features. Developmental trajectories differ significantly between host-use strategies (niches), and correlate with distinct clades. In two-host and three-host species using large hosts at early life stages, developmental change is heterotopically accelerated (greater cell mass development) before the first off-host period where selection probably favours large individuals able to better resist dehydration when questing (waiting) for less abundant, less active hosts. In other species, development is heterotopically reduced (neotenic), possibly because dehydration risk is bypassed by prolonged host attachment (one-host species – heterotopic neoteny), or is allometrically repatterned possibly by using highly abundant and active hosts (three-host species using small hosts at early life stages – allometric repatterning). These findings highlight complex trade-offs between on- and off-host factors of free-living ectoparasite ecology, which mediate responses to diverse selection pressures varied by life stage and host-use strategy. It is proposed that these trade-offs shape evolutionary-developmental morphology and diversity of Rhipicephalus ticks.

Termites sense tiny substrate-borne vibrations through subgenual organs (SGOs) located within their legs' tibiae. Little is known about the SGOs' structure and physical properties. We applied high-resolution (voxel size 0.45 μm) micro-computed tomography (μCT) to Australian termites, Coptotermes lacteus and Nasutitermes exitiosus (Hill) to test two staining techniques. We compared the effectiveness of a single stain of Lugol's iodine solution (LS) to LS followed by Phosphotungstic acid (PTA) solutions (1% and 2%). We then present results of a soldier of Nasutitermes exitiosus combining μCT with LS + 2%PTS stains and scanning electron microscopy to exemplify the visualisation of their SGOs. The termite's SGO due to its approximately oval shape was shown to have a maximum diameter of 60 μm and a minimum of 48 μm, covering 60 ± 4% of the leg's cross-section and 90.4 ± 5% of the residual haemolymph channel. Additionally, the leg and residual haemolymph channel cross-sectional area decreased around the SGO by 33% and 73%, respectively. We hypothesise that this change in cross-sectional area amplifies the vibrations for the SGO. Since SGOs are directly connected to the cuticle, their mechanical properties and the geometric details identified here may enable new approaches to determine how termites sense micro-vibrations.