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Biomechanical analysis of fixation methods for bone flap repositioning after lateral orbitotomy approach: A finite element analysis 眶外侧切口术后骨瓣复位固定方法的生物力学分析:有限元分析
IF 1.8 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.jormas.2024.101938

Objective

In ophthalmic surgery, different materials and fixation methods are employed for bone flap repositioning after lateral orbitotomy approach (LOA), yet there is no unified standard. This study aims to investigate the impact of different fixation strategies on orbital stability through Finite Element Analysis (FEA) simulations of the biomechanical environment for orbital rim fixation in LOA.

Methods

A Finite Element Model (FEM) was established and validated to simulate the mechanical responses under various loads in conventional lateral orbitotomy approach (CLOA) and deep lateral orbital decompression (DLOD) using single titanium plate, double titanium plates, and double absorbable plates fixation methods. The simulations were then validated against clinical cases.

Results

Under similar conditions, the maximum equivalent stress (MES) on titanium alloy fixations was greater than that on absorbable plate materials. Both under static and physiological conditions, all FEM groups ensured structural stability of the system, with material stresses remaining within safe ranges. Compared to CLOA, DLOD, which involves the removal of the lateral orbital wall, altered stress conduction, resulting in an increase of MES and maximum total deformation (MTD) by 1.96 and 2.62 times, respectively. Under a horizontal load of 50 N, the MES in FEM/DLOD exceeded the material's own strength, with an increase in MES and MTD by 3.18 and 6.64 times, respectively, compared to FEM/CLOA. Under a vertical force of 50 N, the MES sustained by each FEM was within safe limits. Bone flap rotation angles remained minimally varied across scenarios. During follow-up, the 12 patients validated in this study did not experience complications related to the internal fixation devices.

Conclusion

Under static or physiological conditions, various fixation methods can effectively maintain stability at the orbitotomy site, and absorbable materials, with their smoother stress transmission properties, are more suited for application in CLOA. Among titanium plate fixations, single titanium plates can better withstand vertical stress, while double titanium plates are more capable of handling horizontal stress. Given the change in the orbital mechanical behavior due to DLOD, enhanced fixation strength should be considered for bone flap repositioning.
目的:在眼科手术中,眼眶外侧切开术(LOA)后的骨瓣复位采用了不同的材料和固定方法,但目前尚无统一标准。本研究旨在通过有限元分析(FEA)模拟 LOA 中眶缘固定的生物力学环境,研究不同固定策略对眼眶稳定性的影响:方法:建立并验证了有限元模型(FEM),以模拟在常规外侧眶切开术(CLOA)和深外侧眶减压术(DLOD)中使用单钛板固定、双钛板固定和双可吸收板固定方法时各种载荷下的机械响应。然后根据临床病例对模拟结果进行了验证:结果:在类似条件下,钛合金固定材料的最大等效应力(MES)大于可吸收钢板材料。在静态和生理条件下,所有有限元组都能确保系统结构的稳定性,材料应力保持在安全范围内。与CLOA相比,DLOD涉及到切除眶外侧壁,改变了应力传导,导致MES和最大总变形(MTD)分别增加了1.96倍和2.62倍。在 50N 的水平荷载下,FEM/DLOD 的 MES 超过了材料自身的强度,与 FEM/CLOA 相比,MES 和 MTD 分别增加了 3.18 倍和 6.64 倍。在 50N 的垂直力作用下,每个 FEM 所承受的 MES 都在安全范围内。骨瓣旋转角度在不同情况下变化很小。在随访期间,本研究验证的 12 名患者均未出现与内固定装置相关的并发症:结论:在静态或生理条件下,各种固定方法都能有效保持眼眶切开部位的稳定性,而可吸收材料具有更平滑的应力传递特性,更适合应用于 CLOA。在钛板固定中,单钛板更能承受垂直应力,而双钛板更能承受水平应力。鉴于 DLOD 导致的眼眶机械行为的改变,在骨瓣重新定位时应考虑增强固定强度。
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引用次数: 0
Minimum follow-up for closed rhinoseptoplasties 闭合性鼻骨整形术的最少随访时间。
IF 1.8 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.jormas.2024.101966

Background

There is no established consensus on the postoperative follow-up from which the aesthetic and functional outcomes of rhinoseptoplasty are considered as stable.

Objectives

To contribute to defining the postoperative follow-up from which rhinoseptoplasty outcomes cease to evolve.

Methods

Postoperative assessments of Nasal Obstruction Symptom Evaluation (NOSE) and Rhinoplasty Outcome Evaluation (ROE) scores from 357 closed structural rhinoseptoplasty procedures were prospectively gathered from January 2019 to December 2023. These measurements encompassed the postoperative period from 1 to 12 months. All procedures were performed utilizing closed technique.

Results

: No statistically significant difference was detected between the scores at 1, 2, and 6 months versus 12 months postoperatively (ROE: p = 0.388; 0.268; 0.162; NOSE: p = 0.265; 0.192; 0.975, Mann-Whitney test). Similarly, no follow-up impact was revealed between the scores at 1, 2, 6, and 12 months postoperatively (ROE: p = 0.548; NOSE: p = 0.280, Kruskal-Wallis test). No significant correlation was established between follow-up (in months) and ROE and NOSE scores (ROE: p = 0.397; NOSE: p = 0.632, Spearman).

Conclusion

: Follow-up duration does not influence NOSE and ROE scores over the 1- to 12-month timeframe. The 1-month postoperative outcome can be regarded as a reliable indicator of the 12-month outcome. These conclusions apply to NOSE and ROE scores of rhinoseptoplasty conducted using closed technique for the 1- to 12-month period. Further research is needed for open techniques, preservation rhinoplasty, other patient-reported outcomes measures (PROMs) as well as for the follow-up beyond 12 months postoperatively.
背景:关于鼻骨整形术的术后随访,目前尚未达成一致意见:关于鼻中隔成形术的美学和功能结果稳定的术后随访时间,目前还没有达成共识:目的:帮助确定鼻中隔成形术的术后随访结果:方法:从2019年1月至2023年12月,对357例闭合结构性鼻中隔成形术的术后鼻阻塞症状评估(NOSE)和鼻成形术结果评估(ROE)评分进行了前瞻性收集。这些测量包括术后 1 至 12 个月。所有手术均采用闭合技术进行:术后 1、2 和 6 个月的评分与术后 12 个月的评分之间没有发现明显的统计学差异(ROE:P = 0.388;0.268;0.162;NOSE:P = 0.265;0.192;0.975,Mann-Whitney 检验)。同样,术后 1 个月、2 个月、6 个月和 12 个月的评分之间也没有发现后续影响(ROE:P = 0.548;NOSE:P = 0.280,Kruskal-Wallis 检验)。随访时间(以月为单位)与ROE和NOSE评分之间无明显相关性(ROE:p = 0.397;NOSE:p = 0.632,Spearman):结论:随访时间不会影响 1 至 12 个月的 NOSE 和 ROE 分数。术后 1 个月的结果可被视为 12 个月结果的可靠指标。这些结论适用于采用闭合技术进行鼻骨整形术后1至12个月的NOSE和ROE评分。对于开放式技术、保存式鼻整形术、其他患者报告的结果测量(PROMs)以及术后 12 个月后的随访,还需要进一步研究。
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引用次数: 0
Intra-arterial chemoradiotherapy for oral cancer: Superiority of intensity-modulated radiation therapy over three-dimensional conformal radiation therapy 口腔癌动脉内化学放疗:调强放疗优于三维适形放疗。
IF 1.8 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.jormas.2024.101981
Makoto Ito , Tomio Hayashi , Arisa Takeuchi , Souichirou Abe , Sou Adachi , Yukihiko Oshima , Yoshiaki Kazaoka , Kojiro Suzuki

Purpose

To determine the superiority of intensity-modulated radiation therapy (IMRT) over three-dimensional conformal radiation therapy (3DCRT) in patients who underwent intra-arterial chemoradiotherapy for oral cancer.

Methods

We retrospectively analyzed patients with locally advanced oral cancer curatively treated with intra-arterial chemoradiotherapy at a single institution between 2010 and 2021. All patients treated after May 2015 underwent IMRT. Docetaxel (12 mg/m2/week) and nedaplatin (5 mg/m2/day) were administered through a shallow temporal artery using a catheter.

Results

In total, 143 patients (IMRT: 71; 3DCRT: 72) were included in this study. The 5-year overall survival (OS) and progression-free survival (PFS) rates were 75.7 % and 59.8 %, respectively, with no significant differences between the irradiation methods. In multivariate analysis, cervical lymph node metastasis (LNM) was the only significant poor prognostic factor contributing to OS, PFS, locoregional control (LRC), and local control (LC). In multivariate subgroup analysis of LNM cases (n = 90), IMRT contributed to favorable LRC (hazard ratio [HR]=0.4, P = 0.01) and LC (HR=0.4, P = 0.006). There was no difference in the incidence of grade ≥2 osteonecrosis of the jaw (4.2 % vs. 12.5 %, P = 0.13), xerostomia (75 % vs. 82 %, P = 0.316), or dysgeusia (80 % vs. 82 %, P = 0.834) between the IMRT and 3DCRT groups. However, the rates of xerostomia at 6 months and dysgeusia at 3 months were lower in the IMRT group (both P < 0.001).

Conclusion

IMRT neither improved patient survival nor significantly reduced the incidence of osteonecrosis of the jaw. However, it demonstrated favorable LRC and LC in patients with LNM, suggesting an advantage in early recovery from xerostomia and dysgeusia.
目的:确定在接受动脉内化学放疗的口腔癌患者中,调强放射治疗(IMRT)优于三维适形放射治疗(3DCRT):我们回顾性分析了2010年至2021年间在一家机构接受动脉内放化疗的局部晚期口腔癌患者。所有在 2015 年 5 月之后接受治疗的患者均接受了 IMRT。多西他赛(12 毫克/平方米/周)和奈达铂(5 毫克/平方米/天)使用导管通过颞浅动脉给药:共有143名患者(IMRT:71人;3DCRT:72人)参与了这项研究。5年总生存率(OS)和无进展生存率(PFS)分别为75.7%和59.8%,两种照射方法之间无显著差异。在多变量分析中,宫颈淋巴结转移(LNM)是影响OS、PFS、局部控制(LRC)和局部控制(LC)的唯一重要不良预后因素。在对LNM病例(90例)进行的多变量亚组分析中,IMRT有助于提高LRC(危险比[HR]=0.4,P=0.01)和LC(HR=0.4,P=0.006)。IMRT组和3DCRT组之间≥2级颌骨坏死(4.2% vs. 12.5%,P=0.13)、口干(75% vs. 82%,P=0.316)或口臭(80% vs. 82%,P=0.834)的发生率没有差异。然而,IMRT 组患者 6 个月时的口干症发生率和 3 个月时的吞咽困难发生率较低(均为 PC结论:IMRT既没有提高患者的生存率,也没有显著降低颌骨坏死的发生率。然而,IMRT在LNM患者的LRC和LC方面表现良好,这表明它在早期恢复口腔排泄和口腔异味方面具有优势。
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引用次数: 0
Augmented reality in implantology: Virtual surgical checklist and augmented implant placement 植入学中的增强现实技术:虚拟手术清单和增强植入物植入。
IF 1.8 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.jormas.2024.101813

Objectives

Aim of the present study was to create a pedagogical checklist for implant surgical protocol with an augmented reality (AR) guided freehand surgery to inexperienced surgeons using a head mounted display (HMD) with tracking.

Methods

The anatomical model of a patient with two missing mandibular teeth requiring conventional single-tooth implants was selected. The computed tomography (CT) scans were extracted and imported into segmentation and implant planning software. A Patient-specific dental splint through an intermediate strut, supported 3D-printed QR code. A checklist was generated to guide surgical procedure. After tracking, the AR-HMD projects the virtual pre-surgical plan (inferior alveolar nerve (IAN), implant axis, implant location) onto the real 3D-printed anatomical models. The entire drilling sequence was based on the manufacturer's recommendations, on 3D-printed anatomical models. After the implant surgical procedure, CT of the 3D-printed models was performed to compare the actual and simulated implant placements. All procedures in the study were performed in accordance with the Declaration of Helsinki.

Results

In total, two implants were placed in a 3D-printed anatomical model of a female patient who required implant rehabilitation for dental agenesis at the second mandibular premolar positions (#35 and #45). Superimposition of the actual and simulated implants showed high concordance between them.

Conclusion

AR in education offers crucial surgical information for novice surgeons in real time. However, the benefits provided by AR in clinical and educational implantology must be demonstrated in other studies involving a larger number of patients, surgeons and apprentices.
目的:本研究的目的是通过使用带有跟踪功能的头戴式显示器(HMD),为缺乏经验的外科医生创建一份由增强现实(AR)引导的徒手种植手术教学清单:方法:选取了一名下颌两颗牙齿缺失、需要进行传统单牙种植的患者的解剖模型。提取计算机断层扫描(CT)扫描结果并导入分割和种植规划软件。通过中间支柱制作了患者专用的牙科夹板,支持 3D 打印 QR 代码。生成的检查表可指导手术过程。跟踪后,AR-HMD 将虚拟手术前计划(下牙槽神经 (IAN)、种植体轴线、种植体位置)投射到真实的 3D 打印解剖模型上。整个钻孔过程都是根据制造商的建议在 3D 打印的解剖模型上进行的。种植手术结束后,对 3D 打印模型进行 CT 扫描,以比较实际和模拟的种植位置。研究中的所有程序均按照《赫尔辛基宣言》进行:一名女性患者因牙齿缺失需要在第二下颌前磨牙位置(35 号和 45 号)进行种植修复,在该患者的三维打印解剖模型中总共植入了两颗种植体。实际种植体与模拟种植体的叠加显示两者之间高度一致:AR教育为外科医生新手提供了重要的实时手术信息。然而,AR 在临床和教育种植学方面的优势必须在其他涉及更多患者、外科医生和学徒的研究中得到证实。
{"title":"Augmented reality in implantology: Virtual surgical checklist and augmented implant placement","authors":"","doi":"10.1016/j.jormas.2024.101813","DOIUrl":"10.1016/j.jormas.2024.101813","url":null,"abstract":"<div><h3>Objectives</h3><div>Aim of the present study was to create a pedagogical checklist for implant surgical protocol with an augmented reality (AR) guided freehand surgery to inexperienced surgeons using a head mounted display (HMD) with tracking.</div></div><div><h3>Methods</h3><div>The anatomical model of a patient with two missing mandibular teeth requiring conventional single-tooth implants was selected. The computed tomography (CT) scans were extracted and imported into segmentation and implant planning software. A Patient-specific dental splint through an intermediate strut, supported 3D-printed QR code. A checklist was generated to guide surgical procedure. After tracking, the AR-HMD projects the virtual pre-surgical plan (inferior alveolar nerve (IAN), implant axis, implant location) onto the real 3D-printed anatomical models. The entire drilling sequence was based on the manufacturer's recommendations, on 3D-printed anatomical models. After the implant surgical procedure, CT of the 3D-printed models was performed to compare the actual and simulated implant placements. All procedures in the study were performed in accordance with the Declaration of Helsinki.</div></div><div><h3>Results</h3><div><span>In total, two implants were placed in a 3D-printed anatomical model of a female patient who required implant rehabilitation for dental agenesis at the </span>second mandibular premolar positions (#35 and #45). Superimposition of the actual and simulated implants showed high concordance between them.</div></div><div><h3>Conclusion</h3><div>AR in education offers crucial surgical information for novice surgeons in real time. However, the benefits provided by AR in clinical and educational implantology must be demonstrated in other studies involving a larger number of patients, surgeons and apprentices.</div></div>","PeriodicalId":55993,"journal":{"name":"Journal of Stomatology Oral and Maxillofacial Surgery","volume":"125 5","pages":"Article 101813"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140061428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Eyelid and periorbital necrotizing fasciitis, a severe preseptal infection, a systemic review of the literature and anatomical illustrations 眼睑和眶周坏死性筋膜炎--一种严重的睑板前感染,文献和解剖图解的系统回顾。
IF 1.8 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.jormas.2023.101498
In necrotizing fasciitis, aggressive surgical debridement and broad-spectrum antibiotics are the cornerstone of treatment but cannot be proposed for the eyelid and periorbital area because of the risk of blindness, eyeball exposure and disfiguration. The aim of this review was to determine the most effective management of this severe infection while preserving eye function. A literature search of the PubMed, Cochrane Library, ScienceDirect and Embase databases was conducted for all articles published up to March 2022; 53 patients were included.
Management was probabilistic, combining antibiotic therapy with skin (+/- orbicularis oculi muscle) debridement in 67.9% of cases, and probabilistic antibiotic therapy alone in 16.9% of cases. Radical surgery with exenteration was performed in 11.1% of patients; 20.9% of patients had complete loss of vision, and 9.4% died of the disease. Aggressive debridement was rarely necessary possibly because of the anatomical particularities of this region.
在坏死性筋膜炎中,积极的手术清创和广谱抗生素是治疗的基础,但由于眼睑和眶周区域存在失明、眼球暴露和毁容的风险,因此不能建议对其进行治疗。本综述旨在确定治疗这种严重感染的最有效方法,同时保护眼部功能。我们对 PubMed、Cochrane Library、ScienceDirect 和 Embase 数据库中截至 2022 年 3 月发表的所有文章进行了文献检索,共纳入 53 例患者。67.9%的病例采用概率性治疗,结合抗生素治疗和皮肤(+/-眼轮匝肌)清创,16.9%的病例仅采用概率性抗生素治疗。11.1%的患者接受了根治性手术和外展手术;20.9%的患者视力完全丧失,9.4%的患者死于该病。可能是由于该区域解剖结构的特殊性,很少需要进行积极的清创手术。
{"title":"Eyelid and periorbital necrotizing fasciitis, a severe preseptal infection, a systemic review of the literature and anatomical illustrations","authors":"","doi":"10.1016/j.jormas.2023.101498","DOIUrl":"10.1016/j.jormas.2023.101498","url":null,"abstract":"<div><div><span>In necrotizing fasciitis, aggressive surgical </span>debridement<span><span> and broad-spectrum antibiotics are the cornerstone of treatment but cannot be proposed for the eyelid and periorbital area because of the risk of blindness, </span>eyeball exposure and disfiguration. The aim of this review was to determine the most effective management of this severe infection while preserving eye function. A literature search of the PubMed, Cochrane Library, ScienceDirect and Embase databases was conducted for all articles published up to March 2022; 53 patients were included.</span></div><div>Management was probabilistic, combining antibiotic therapy with skin (+/- orbicularis oculi muscle) debridement in 67.9% of cases, and probabilistic antibiotic therapy alone in 16.9% of cases. Radical surgery with exenteration was performed in 11.1% of patients; 20.9% of patients had complete loss of vision, and 9.4% died of the disease. Aggressive debridement was rarely necessary possibly because of the anatomical particularities of this region.</div></div>","PeriodicalId":55993,"journal":{"name":"Journal of Stomatology Oral and Maxillofacial Surgery","volume":"125 5","pages":"Article 101498"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9824012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The dangers of excessive cosmetic procedures: Finding balance in pursuit of beauty 过度美容的危害:在追求美丽的过程中寻找平衡。
IF 1.8 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.jormas.2024.101926
{"title":"The dangers of excessive cosmetic procedures: Finding balance in pursuit of beauty","authors":"","doi":"10.1016/j.jormas.2024.101926","DOIUrl":"10.1016/j.jormas.2024.101926","url":null,"abstract":"","PeriodicalId":55993,"journal":{"name":"Journal of Stomatology Oral and Maxillofacial Surgery","volume":"125 5","pages":"Article 101926"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141201279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pathogenic loss-of-function mutations in LRP1B are associated with poor survival in head and neck cancer patients LRP1B 的致病性功能缺失突变与头颈癌患者的不良生存率有关。
IF 1.8 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.jormas.2024.101971
Paramasivam Arumugam , Senthil Murugan M , Vijayashree Priyadharsini Jayaseelan

Objective

Head and neck squamous cell carcinoma (HNSCC) present a significant challenge in the medical field due to treatment resistance, which often hinders successful outcomes. The dysregulation of the LRP1B gene is linked to various cancers, but its specific role in HNSCC is poorly understood.

Methods

This study investigated the link between pathogenic loss-of-function mutations in the LRP1B gene and survival outcomes in HNSCC patients. The Cancer Genome Atlas HNSCC cohort, comprised of 520 tumor and 44 normal tissues, was analyzed using cBioportal, and UALCAN tools. Expression patterns, survival outcomes, and clinical correlations of LRP1B were evaluated. In-depth analyses involved validation of mRNA expression using RT-qPCR and functional exploration using various in-silico tools.

Results

Analysis of data from The Cancer Genome Atlas (TCGA) and cBioPortal revealed a high frequency (25 %) of LRP1B mutations in HNSCC patients. Notably, splice mutation, truncating mutation, and deep deletion, considered potential drivers, are commonly associated with LRP1B mutations. Patients with LRP1B mutations also exhibit poorer overall survival rates compared to those without these mutations. Furthermore, LRP1B mRNA expression is significantly reduced in HNSCC tissues compared to normal tissues and is correlated with advanced tumor stage, higher tumor grade, and nodal metastasis.

Conclusion

These findings indicate that LRP1B may function as both a prognostic biomarker and a therapeutic target in HNSCC patients.
目的:头颈部鳞状细胞癌(HNSCC)因其耐药性而成为医学领域的一大挑战,耐药性常常阻碍治疗的成功。LRP1B 基因的失调与多种癌症有关,但其在 HNSCC 中的具体作用却鲜为人知:本研究调查了 LRP1B 基因的致病性功能缺失突变与 HNSCC 患者生存结果之间的联系。研究使用 cBioportal 和 UALCAN 工具分析了癌症基因组图谱 HNSCC 队列(由 520 个肿瘤组织和 44 个正常组织组成)。评估了 LRP1B 的表达模式、生存结果和临床相关性。深入分析包括使用 RT-qPCR 验证 mRNA 表达,以及使用各种内部工具进行功能探索:对癌症基因组图谱(TCGA)和 cBioPortal 数据的分析表明,LRP1B 在 HNSCC 患者中的突变频率很高(25%)。值得注意的是,剪接突变、截短突变和深度缺失被认为是潜在的驱动因素,它们通常与LRP1B突变相关。与无LRP1B突变的患者相比,LRP1B突变患者的总生存率也较低。此外,与正常组织相比,LRP1B mRNA在HNSCC组织中的表达明显减少,并且与肿瘤晚期、肿瘤分级较高和结节转移相关:这些研究结果表明,LRP1B 可作为 HNSCC 患者的预后生物标志物和治疗靶点。
{"title":"Pathogenic loss-of-function mutations in LRP1B are associated with poor survival in head and neck cancer patients","authors":"Paramasivam Arumugam ,&nbsp;Senthil Murugan M ,&nbsp;Vijayashree Priyadharsini Jayaseelan","doi":"10.1016/j.jormas.2024.101971","DOIUrl":"10.1016/j.jormas.2024.101971","url":null,"abstract":"<div><h3>Objective</h3><div>Head and neck squamous cell carcinoma (HNSCC) present a significant challenge in the medical field due to treatment resistance, which often hinders successful outcomes. The dysregulation of the <em>LRP1B</em> gene is linked to various cancers, but its specific role in HNSCC is poorly understood.</div></div><div><h3>Methods</h3><div>This study investigated the link between pathogenic loss-of-function mutations in the <em>LRP1B</em> gene and survival outcomes in HNSCC patients. The Cancer Genome Atlas HNSCC cohort, comprised of 520 tumor and 44 normal tissues, was analyzed using cBioportal, and UALCAN tools. Expression patterns, survival outcomes, and clinical correlations of <em>LRP1B</em> were evaluated. In-depth analyses involved validation of mRNA expression using RT-qPCR and functional exploration using various <em>in-silico</em> tools.</div></div><div><h3>Results</h3><div>Analysis of data from The Cancer Genome Atlas (TCGA) and cBioPortal revealed a high frequency (25 %) of <em>LRP1B</em> mutations in HNSCC patients. Notably, splice mutation, truncating mutation, and deep deletion, considered potential drivers, are commonly associated with <em>LRP1B</em> mutations. Patients with <em>LRP1B</em> mutations also exhibit poorer overall survival rates compared to those without these mutations. Furthermore, <em>LRP1B</em> mRNA expression is significantly reduced in HNSCC tissues compared to normal tissues and is correlated with advanced tumor stage, higher tumor grade, and nodal metastasis.</div></div><div><h3>Conclusion</h3><div>These findings indicate that LRP1B may function as both a prognostic biomarker and a therapeutic target in HNSCC patients.</div></div>","PeriodicalId":55993,"journal":{"name":"Journal of Stomatology Oral and Maxillofacial Surgery","volume":"125 5","pages":"Article 101971"},"PeriodicalIF":1.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Netrin 1 as a biomarker in cancer: scoping diagnostic, prognostic, and therapeutic perspectives with a focus on oral squamous cell carcinoma 作为癌症生物标记物的内皮素 1:以口腔鳞状细胞癌为重点,探讨诊断、预后和治疗前景。
IF 1.8 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.jormas.2024.101982
Hema Shree K , Gayathri R , Vishnu Priya Veeraraghavan , Pratibha Ramani
Goal of the review: The utilization of biomarkers to predict cancer risk, prognosis, and treatment outcomes is paramount. Netrin-1 (NTN1), known for its role in commissural axon guidance during embryonic development, has emerged as a versatile molecule with significant implications in cancer and neurobiology. Structurally resembling laminin, Netrin-1 regulates neuronal connectivity and plasticity in adulthood, influencing axonal and dendritic growth, neurotransmission, and cell migration. In addition to its neurological functions, Netrin-1 is increasingly recognized for its involvement in maintaining epithelial tissue and its regulatory roles in fundamental cellular processes, including adhesion, proliferation, differentiation, apoptosis, and angiogenesis.
In cancer biology, Netrin-1′s interactions with its receptors, such as DCC [Deleted in Colorectal Cancer] and UNC5 (a homolog of DCC), have been implicated in tumor progression across various physiological systems. Elevated levels of Netrin-1 in colorectal cancer and head and neck squamous cell carcinoma are correlated with increased tumorigenic potential, mediated through pathways involving NFκB activation and anti-apoptotic mechanisms. Mechanically induced hypermethylation and downstream signaling cascades that inhibit apoptosis and promote cell survival are observed upon Netrin-1 binding to DCC.
Furthermore, Netrin-1 shows promise as a biomarker for detecting inflammatory activity in diseases such as multiple sclerosis and as a potential diagnostic, prognostic, and therapeutic indicator in oral squamous cell carcinoma. Elevated levels of Netrin-1 in bodily fluids, alongside immunohistochemical evidence, support its potential as a valuable clinical marker in cancer management.
This abstract emphasizes Netrin-1′s diverse biological roles, underscoring its potential as a diagnostic tool and therapeutic target in cancer research. The need for further exploration of Netrin-1′s molecular interactions and clinical applications is urgent and crucial to advance personalized medicine approaches and enhance patient outcomes in oncology and neurology.
综述的目的:利用生物标志物预测癌症风险、预后和治疗效果至关重要。Netrin-1(NTN1)因其在胚胎发育过程中的神经轴突导向作用而闻名,现已成为对癌症和神经生物学有重要影响的多功能分子。Netrin-1在结构上类似于层粘蛋白,可调节成年期神经元的连接性和可塑性,影响轴突和树突的生长、神经传递和细胞迁移。除了神经功能外,Netrin-1 还参与维持上皮组织,并在粘附、增殖、分化、凋亡和血管生成等基本细胞过程中发挥调控作用,这一点正日益得到认可。在癌症生物学中,Netrin-1 与其受体(如 DCC [大肠癌中删除的受体] 和 UNC5(DCC 的同源物))的相互作用已被认为与各种生理系统的肿瘤进展有关。在结直肠癌和头颈部鳞状细胞癌中,Netrin-1 水平的升高与致瘤潜能的增加有关,它是通过涉及 NFκB 激活和抗凋亡机制的途径介导的。当 Netrin-1 与 DCC 结合时,可观察到机械诱导的高甲基化和抑制细胞凋亡、促进细胞存活的下游信号级联。此外,Netrin-1有望成为检测多发性硬化症等疾病中炎症活动的生物标记物,以及口腔鳞状细胞癌的潜在诊断、预后和治疗指标。体液中 Netrin-1 水平的升高以及免疫组化证据都支持其在癌症治疗中作为有价值的临床标记物的潜力。本摘要强调了 Netrin-1 的多种生物学作用,突出了其作为癌症研究中的诊断工具和治疗靶点的潜力。迫切需要进一步探索 Netrin-1 的分子相互作用和临床应用,这对推进肿瘤学和神经病学的个性化医疗方法和提高患者预后至关重要。
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引用次数: 0
‘Mini DC-F’ in rhinoplasty: Diced cartilage and fascia grafts with the temporalis fascia dissected along its width – A technical note 鼻整形术中的 "迷你 DC-F":切割软骨和筋膜移植,沿其宽度剖开颞肌筋膜--技术说明。
IF 1.8 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.jormas.2024.101969
JB Caruhel , P Moulin , A Crambert , A Deygat
In rhinoplasty, diced cartilage with fascia (DC-F) is a commonly used technique to enhance dorsal projection and achieve a smooth dorsum. We have developed a modified technique by dissecting the thickness of the fascia along its width, called “Mini DC-F”. This method allows for effective camouflaging of dorsal irregularities and provides a slight increase in dorsal height. This simplified and less invasive technique not only reduces operative time but also enhances the surgeon's ability to achieve a refined aesthetic result. This technique offers an intermediate option in the surgical arsenal, bridging the gap between pure camouflaging methods and those aimed at significant dorsal augmentation.
在鼻整形术中,带筋膜的软骨切片(DC-F)是一种常用的技术,可增强鼻背的突出度并获得光滑的鼻背。我们开发了一种改良技术,沿筋膜宽度剖开筋膜厚度,称为 "迷你 DC-F"。这种方法可以有效掩盖背侧的不规则,并使背侧高度略有增加。这种简化的微创技术不仅缩短了手术时间,还提高了外科医生获得精致美观效果的能力。这项技术为外科手术提供了一个中间选择,在纯粹的伪装方法和旨在显著增加背高的方法之间架起了一座桥梁。
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引用次数: 0
Correlation between the three-dimensional hyoid bone parameters and pharyngeal airway dimensions in different sagittal and vertical malocclusions 不同矢状畸形和垂直畸形的舌骨三维参数与咽部气道尺寸之间的相关性。
IF 1.8 3区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2024-10-01 DOI: 10.1016/j.jormas.2024.101994
Majedh Abdo Ali Al-Somairi , Bowen Zheng , Bushra Sufyan Almaqrami , Naseem Ali Al-Worafi , Enas Senan Alyafrusee , Barakat Al-Tayar , Remsh K. Al-Rokhami , Leena Ali Al-Warafi , Najah Alhashimi , Hao Xu , Yi Liu

Objective

This study aimed to explore the relationship between three-dimensional (3D) measurements of the hyoid bone (HB) and pharyngeal airway space (PAS) in relation to sagittal and vertical malocclusion.

Methods

A total of 368 cone-beam computed tomography (CBCT) scans were classified into three skeletal groups (Class I, II, and III) and subdivided by vertical growth patterns (hypodivergent, normodivergent, and hyperdivergent). PAS dimensions, including nasopharyngeal, oropharyngeal, hypopharyngeal, and total airway spaces, were measured in surface area, volume, minimum constricted area (MCA), length, and width, HB position and dimension were analyzed in 3D using InVivo 6.0.3 and Dolphin 11.8 software. Data were analyzed using two-way ANOVA, and Bonferroni post-hoc tests, with P ≤ 0.05 considered significant.

Results

The study found that patients with skeletal Class III and hypodivergent growth pattern had the highest sagittal position of the hyoid bone, while those with skeletal Class II and hyperdivergent pattern had the lowest hyoid length. Nasopharyngeal airway space width was significantly lower in skeletal Class III patients, while volume and area were lower in hyperdivergent patients. Oropharyngeal and hypopharyngeal dimensions were also affected by skeletal class and growth pattern, with hyperdivergent patients having the lowest values. Total pharyngeal volume, area, and minimum constricted area were also affected, with hyperdivergent patients having the lowest values and skeletal Class II patients having the lowest minimum constricted area.

Conclusion

Pharyngeal airway dimensions and hyoid bone parameters vary with malocclusions. The hyoid bone's position influences the airway, identifying patients at risk for airway obstruction and sleep-disordered breathing.
研究目的本研究旨在探讨舌骨(HB)和咽气道间隙(PAS)的三维(3D)测量值与矢状和垂直错颌畸形之间的关系:将总共 368 个锥形束计算机断层扫描(CBCT)扫描结果分为三个骨骼组(I 级、II 级和 III 级),并按垂直生长模式(低发散、正常发散和高发散)进行细分。用 InVivo 6.0.3 和 Dolphin 11.8 软件测量了包括鼻咽、口咽、下咽和总气道空间在内的 PAS 尺寸的表面积、体积、最小收缩面积(MCA)、长度和宽度,并对 HB 位置和尺寸进行了三维分析。数据采用双因素方差分析和Bonferroni事后检验,P≤0.05为显著:研究发现,骨骼Ⅲ级和生长模式低发散的患者舌骨矢状位最高,而骨骼Ⅱ级和生长模式高发散的患者舌骨长度最低。骨骼Ⅲ级患者的鼻咽气道间隙宽度明显较低,而超发散型患者的鼻咽气道间隙容积和面积均较低。口咽和下咽的尺寸也受骨骼分级和生长模式的影响,超分化患者的数值最低。咽部总体积、面积和最小收缩面积也受到影响,高分化患者的数值最低,骨骼分级为II级的患者最小收缩面积最低:结论:咽部气道尺寸和舌骨参数随畸形而变化。舌骨的位置会影响气道,识别气道阻塞和睡眠呼吸障碍的高危患者。
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Journal of Stomatology Oral and Maxillofacial Surgery
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