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Antioxidant Responses of Water-Stressed Cherry Tomato Plants to Natural Biostimulants 水分胁迫下樱桃番茄植株对天然生物刺激物的抗氧化反应
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2023-09-03 DOI: 10.3390/agronomy13092314
R. Gil-Ortiz, M. Naranjo, Sergio Atarés, Oscar Vicente
Biostimulants’ application to plants can reduce the damage caused by abiotic factors such as drought or salinity and improve crop yield under these stressful conditions. In this work, several biostimulants, namely Terrabion Aminovit® (a commercial product based on amino acids), potassium fulvate, humic acids, and a seaweed extract, were applied to cherry tomato plants using fertigation at two doses of 0.2 and 1.0 g L−1. The plants were then subjected to a water stress treatment by completely withholding irrigation for 12 days. After the treatments, all plants were harvested to determine several growth and biochemical parameters. Pre-treatment with all biostimulants protected the tomato plants against dehydration, as indicated by a significant increase in leaf water content compared to the non-irrigated controls. Leaf fresh weight and root water content also increased, except in the plants treated with humic acids, by about 2 fold in plants pre-treated with Terrabion Aminovit® and 1.5 fold in the presence of potassium fulvate and the seaweed extract. The water stress treatment caused a significant increase in leaf proline content, up to 113.6 μmol g−1 DW, approximately 18 fold higher than in well-irrigated control plants; this value was significantly lower in Terrabion Aminovit® pre-treated plants but even higher, ca. 180 μmol g−1 DW, in those treated previously with the seaweed extract. These results indicate that proline is a suitable water stress biomarker in tomatoes and that the biostimulants probably differ in their mode of action, suggesting that the effect of the seaweed extract is mediated by proline accumulation. A significant activation of antioxidant enzymes, namely superoxide dismutase, ascorbate peroxidase, and glutathione reductase, was also observed in water-stressed plants; application of the biostimulants resulted in all cases, in a significant reduction in the specific activities of the three enzymes, indicating reduced levels of drought-induced oxidative stress in the plants. We conclude that applying these biostimulants, particularly Terrabion Aminovit®, may help minimise the adverse effects of water stress on tomatoes by maintaining turgor and improving growth through mechanisms still unknown but which appear to involve, at least in part, enhancing the plants’ antioxidant defence responses.
在植物上施用生物刺激剂可以减少干旱或盐碱等非生物因素造成的损害,并在这些胁迫条件下提高作物产量。在这项工作中,几种生物刺激剂,即Terrabion Aminovit®(一种基于氨基酸的商业产品)、黄酸钾、腐植酸和海藻提取物,以0.2和1.0 g L−1的两种剂量施用于樱桃番茄植株。然后对植株进行水分胁迫处理,完全不灌溉12天。处理结束后,采集所有植株,测定其生长和生化参数。与未灌溉对照相比,叶片含水量显著增加表明,所有生物刺激剂的预处理保护了番茄植株免受脱水。除腐植酸处理外,叶片鲜重和根含水量也增加了约2倍于Terrabion Aminovit®预处理的植物,1.5倍于黄酸钾和海藻提取物。水分胁迫处理显著提高了叶片脯氨酸含量,最高可达113.6 μmol g−1 DW,约为灌水对照植株的18倍;在Terrabion Aminovit®预处理的植物中,该值显着降低,但在先前用海藻提取物处理的植物中,该值更高,约为180 μmol g−1 DW。这些结果表明,脯氨酸是番茄中合适的水分胁迫生物标志物,而这些生物刺激剂的作用方式可能不同,这表明海藻提取物的作用是通过脯氨酸积累介导的。抗氧化酶,即超氧化物歧化酶、抗坏血酸过氧化物酶和谷胱甘肽还原酶,在缺水植物中也有显著的活化;在所有情况下,应用生物刺激剂导致三种酶的特定活性显著降低,表明植物中干旱诱导的氧化应激水平降低。我们的结论是,使用这些生物刺激剂,特别是Terrabion Aminovit®,可能有助于减少水分胁迫对番茄的不利影响,其机制尚不清楚,但似乎至少部分涉及增强植物的抗氧化防御反应。
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引用次数: 0
Elicitor-Mediated Response of Growth, Yield, and Quality of Kalmegh (Andrographis paniculata Wall. ex Nees, Family Acanthaceae) 栀子花生长、产量和品质的激发子介导反应。前麻豆科麻豆科麻豆
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2023-09-02 DOI: 10.3390/agronomy13092313
Pavan Gowda M., A. Sharangi, T. Upadhyay, Nahaa M. Alotaibi, Modhi O. Alotaibi, N. Alshammari, Mohd Saeed
With the objective of studying the influence of elicitors on the growth, yield, and quality of kalmegh, we carried out an investigation for two consecutive years. Nine treatments with three replications were laid out in a completely randomized design (CRD). Chitosan (CHT), yeast extract (YE), jasmone acid (JA), and salicylic acid (SA)were evaluated at different concentrations. The CHT treatment at 1000 ppm exhibited the tallest plant height (73.91 cm) and the highest number of secondary branches (29.07) at the time of harvest. The primary branches and number of leaves per plant were highest with the CHT treatment at 1000 ppm (26.36; 88.32), and were not significantly different with the SA treatment at 200 ppm (26.28; 81.51). The plant spread was the highest with the SAtreatment at 200 ppm (35.46 cm2) and was not significantly different with the CHT treatment at 1000 ppm (35.11 cm2). The CHT and SA sprays did not result in significant changes in yield parameters, but the highest fresh (42.34 g) and dry (18.30) herbage yields per plant were exhibited with the SA treatment at 200 ppm. The highest total chlorophyll (4.459 mg g−1) and total andrographolide (3.494%) contents were recorded after treatment with the SA spray at 200 ppm. A significant and positive improvement in the growth, yield, and quality of kalmegh was noticed with the salicylic acid spray treatment at 200 ppm 30 and 60 days after sowing (DAS), signifying its benefits for the cultivation of kalmegh in terms of high productivity, quality, and better returns for farmers.
为了研究激发子对卡尔梅生长、产量和品质的影响,我们连续两年进行了调查。采用完全随机设计(CRD),共9个治疗,3个重复。对壳聚糖(CHT)、酵母浸膏(YE)、茉莉酸(JA)和水杨酸(SA)在不同浓度下的效果进行了评价。1000ppm的CHT处理在采收时株高最高(73.91 cm),次枝数最多(29.07)。1000 ppm时,单株一次枝数和叶片数最高(26.36;88.32),与200 ppm的SA处理差异不显著(26.28;81.51)。200 ppm (35.46 cm2)处理的植株蔓延率最高,与1000 ppm (35.11 cm2)处理的差异不显著。喷施CHT和SA对产量参数没有显著影响,但在200 ppm的SA处理下,每株鲜草产量最高(42.34 g),干草产量最高(18.30 g)。200 ppm浓度的SA喷雾处理后,总叶绿素(4.459 mg g−1)和总穿心莲内酯(3.494%)含量最高。在播种后30天和60天,200 ppm水杨酸喷雾处理(DAS)对卡尔梅的生长、产量和质量都有显著的积极改善,这表明它对卡尔梅的种植在高生产力、高质量和更好的回报方面有好处。
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引用次数: 0
Advances in Crop Molecular Breeding and Genetics 作物分子育种与遗传学研究进展
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2023-09-01 DOI: 10.3390/agronomy13092311
Wanning Liu, Guan Li, Jiezheng Ying, Zhiyong Li
Selecting crop varieties with high and stable yields, as well as improving quality and economic benefits, has become a long-term topic while facing the continuous increasing population and the adverse effects of environmental changes [...]
在面临人口持续增加和环境变化的不利影响的同时,选择高产稳定的作物品种,提高质量和经济效益,已成为一个长期课题〔…〕
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引用次数: 0
Buckwheat Plant Height Estimation Based on Stereo Vision and a Regression Convolutional Neural Network under Field Conditions 田间条件下基于立体视觉和回归卷积神经网络的荞麦株高估计
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2023-09-01 DOI: 10.3390/agronomy13092312
Jianlong Zhang, Wenwen Xing, Xuefeng Song, Yulong Cui, Wang Li, Decong Zheng
Buckwheat plant height is an important indicator for producers. Due to the decline in agricultural labor, the automatic and real-time acquisition of crop growth information will become a prominent issue for farms in the future. To address this problem, we focused on stereo vision and a regression convolutional neural network (CNN) in order to estimate buckwheat plant height. MobileNet V3 Small, NasNet Mobile, RegNet Y002, EfficientNet V2 B0, MobileNet V3 Large, NasNet Large, RegNet Y008, and EfficientNet V2 L were modified into regression CNNs. Through a five-fold cross-validation of the modeling data, the modified RegNet Y008 was selected as the optimal estimation model. Based on the depth and contour information of buckwheat depth image, the mean absolute error (MAE), root mean square error (RMSE), mean square error (MSE), and mean relative error (MRE) when estimating plant height were 0.56 cm, 0.73 cm, 0.54 cm, and 1.7%, respectively. The coefficient of determination (R2) value between the estimated and measured results was 0.9994. Combined with the LabVIEW software development platform, this method can estimate buckwheat accurately, quickly, and automatically. This work contributes to the automatic management of farms.
荞麦株高是生产者的重要指标。由于农业劳动力的减少,农作物生长信息的自动实时获取将成为未来农场面临的一个突出问题。为了解决这个问题,我们专注于立体视觉和回归卷积神经网络(CNN)来估计荞麦植株高度。将MobileNet V3 Small、NasNet Mobile、RegNet Y002、EfficientNet V2 B0、MobileNet V3 Large、NasNet Large、RegNet Y008和EfficientNet V2 L分别修改为回归cnn。通过对建模数据进行五重交叉验证,选择修正后的RegNet Y008作为最优估计模型。基于荞麦深度图像的深度和轮廓信息,估算株高的平均绝对误差(MAE)、均方根误差(RMSE)、均方误差(MSE)和平均相对误差(MRE)分别为0.56 cm、0.73 cm、0.54 cm和1.7%。估计结果与实测值的决定系数(R2)值为0.9994。结合LabVIEW软件开发平台,该方法能够准确、快速、自动地对荞麦进行估算。这项工作有助于农场的自动化管理。
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引用次数: 0
Special Issue “Recent Advances in Genomics, Genetic Resources Evaluation and Breeding of Cucurbitaceae Crops” 《葫芦科作物基因组学、遗传资源评价与育种研究进展》特刊
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2023-09-01 DOI: 10.3390/agronomy13092308
Ana I. López-Sesé
Recent progress in plant genomic technologies has amounted to a revolution, making a huge set of molecular tools available for use in plant breeding [...]
植物基因组技术的最新进展相当于一场革命,使一套巨大的分子工具可用于植物育种〔…〕
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引用次数: 0
Innovations in Agriculture for Sustainable Agro-Systems 农业创新促进可持续农业系统
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2023-09-01 DOI: 10.3390/agronomy13092309
Christos Lykas, I. Vagelas
Agriculture has changed dramatically and has been improved due to new technologies [...]
由于新技术的出现,农业发生了巨大的变化,并得到了改善[…]
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引用次数: 0
Agricultural Jiaosu Enhances the Stress Resistance of Pak Choi (Brassica rapa L. subsp. chinensis) by Recruiting Beneficial Rhizosphere Bacteria and Altering Metabolic Pathways 农业胶素增强白菜(Brassica rapa L.)的抗逆性。通过招募有益根际细菌和改变代谢途径
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2023-09-01 DOI: 10.3390/agronomy13092310
Xiaoqian Cheng, Youhui Gao, Ziyu Wang, Yafan Cai, Xiaofen Wang
Agricultural Jiaosu (AJ) is a method of recycling agricultural wastes for improving soil properties, promoting plant growth, and enhancing plant stress resistance. However, the underlying mechanism by which AJ improves plant stress resistance needs to be determined. Therefore, in this study, two treatments of AJ spraying and water spraying were set up to determine the enzyme activities related to the stress resistance of pak choi after 30 days of growth, and the potential mechanism of AJ’s influence on the stress resistance of pak choi was revealed by transcriptome, metabolome, and rhizome microbiome analyses. Microbial community analysis revealed that the application of AJ does not alter microbial abundance in the rhizosphere; however, it can improve microbial diversity and enrich Actinobacteriota, Proteobacteria, and Firmicutes in the pak choi rhizosphere. Metabolomic analysis revealed that these phyla were significantly positively correlated, with highly upregulated metabolites. Our findings suggest that AJ recruits beneficial microorganisms (BMs) in the rhizosphere and stimulates the expression of genes and metabolites involved in phenylpropanoid and glucosinolate biosynthesis, as well as glutathione and alpha-linolenic acid metabolism pathways. The use of AJ could considerably minimise the use of pesticides and fertilisers and improve the quality of the ecological environment.
农业胶素(AJ)是利用农业废弃物改善土壤性质、促进植物生长、增强植物抗逆性的一种方法。然而,AJ提高植物抗逆性的潜在机制还有待确定。因此,本研究设置AJ喷施和水喷施两种处理,测定生长30 d后小白菜抗逆性相关酶活性,并通过转录组、代谢组和根茎微生物组分析揭示AJ对小白菜抗逆性影响的潜在机制。微生物群落分析表明,施用AJ对根际微生物丰度没有影响;然而,它可以提高微生物多样性,丰富放线菌门,变形菌门和厚壁菌门在白菜根际。代谢组学分析显示,这些门显著正相关,代谢产物高度上调。我们的研究结果表明,AJ在根际招募有益微生物(BMs),并刺激参与苯丙氨酸和硫代葡萄糖苷生物合成以及谷胱甘肽和α -亚麻酸代谢途径的基因和代谢物的表达。使用AJ可以大大减少农药和化肥的使用,改善生态环境质量。
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引用次数: 0
Organic Manure Significantly Promotes the Growth of Oilseed Flax and Improves Its Grain Yield in Dry Areas of the Loess Plateau of China 黄土高原干旱区有机肥显著促进油麻生长和提高粮食产量
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2023-08-31 DOI: 10.3390/agronomy13092304
Xingkang Ma, Yuhong Gao, Bing Wu, Xingbang Ma, Y. Wang, B. Yan, Zhengjun Cui, Ming Wen, Xue Zhang, Haidi Wang
Organic fertilizers could be useful for agricultural sustainability. Therefore, this study explored green cultivation techniques to improve the grain yield of oilseed flax in dry areas of the Loess Plateau of China. With no fertilization (CK) as the control, the effects of sheep manure (S1: 12.5 t ha−1; S2: 25 t ha−1), poultry manure (C1: 5.8 t ha−1; C2: 11.6 t ha−1), and chemical fertilizers (F1: N 112 kg ha−1, P 75 kg ha−1, K 67.5 kg ha−1; F2: N 225 kg ha−1, P 150 kg ha−1, K 135 kg ha−1) on the growth and development, the grain filling characteristics, and the yield of the Zhangya 2 oilseed flax (Linum usitatisimum L.) variety were compared and analyzed based on a two-factor split plot experiment. The results showed that the application of manure significantly increased the emergence rate (ER) of oilseed flax. Poultry manure increased plant height while sheep manure increased stem diameter. The dry matter production was higher in the 25 t ha−1 sheep manure treatment by 2.47–40.11% compared with that of the other treatments, and it promoted the distribution ratio of dry matter to grains after anthesis. The observed relationship was in accordance with that presented by the logistic equation between grain weight and days after anthesis, and there were significant positive correlations between the 1000-grain weight and the average filling rate (V-ave), the maximum filling rate (V-max), and the growth at the maximum filling rate (W-max). The application of organic manure accelerated the grain filling rate (GFR); under the treatment with 25 t ha−1 sheep manure, V-ave, V-max, and W-max increased by 4.84–22.72%, 1.16–17.54%, and 4.58–22.63%, respectively, and the grain yield and the net income per unit area increased by 6.35–39.25% and 3.04–95.07%, respectively, compared with those under the other treatments. Consequently, the treatment with 25 t ha−1 sheep manure can significantly promote the growth and development of oilseed flax plants, optimize the grain filling characteristics, and increase the grain yield and net income, making it a suitable fertilization technique for oilseed flax in dry areas of the Loess Plateau of China.
有机肥料可能有助于农业的可持续性。因此,本研究探索了在黄土高原干旱地区提高油麻产量的绿色栽培技术。以不施肥(CK)为对照,研究了羊粪(S1:12.5t ha−1;S2:25t ha−1)、鸡粪(C1:5.8t ha−2;C2:11.6t ha−3)和化肥(F1:N112kg ha−1、P75kg ha−1,K67.5kg ha–1;F2:N225kg ha−2、P150kg ha−2,以张掖2号油麻(Linum usitatiisimum L.)为试材,采用双因素分块试验对其产量进行了比较分析。结果表明,施用有机肥显著提高了油麻的出苗率。鸡粪增加株高,羊粪增加茎粗。与其他处理相比,25t ha−1羊粪处理的干物质产量提高了2.47–40.11%,并促进了花后干物质在籽粒中的分配比例。观察到的关系符合粒重与花后天数之间的logistic方程,1000粒重与平均灌浆速率(V-ave)、最大灌浆速率(Vmax)和最大灌浆速率下的生长(W-max)之间存在显著的正相关。施用有机肥可提高籽粒灌浆速率;与其他处理相比,25t ha−1羊粪处理的V-ave、V-max和W-max分别提高了4.84–22.72%、1.16–17.54%和4.58–22.63%,粮食产量和单位面积净收入分别提高了6.35–39.25%和3.04–95.07%。因此,25t ha−1羊粪处理可显著促进油麻植株的生长发育,优化籽粒灌浆特性,提高粮食产量和净收入,是我国黄土高原干旱区油麻适宜的施肥技术。
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引用次数: 0
Improvement of Fruit Quality and Phytochemical Components of Pomegranate by Spraying with B2O3 and ZnO Nanoparticles 纳米B2O3和ZnO对石榴果实品质和植物化学成分的影响
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2023-08-31 DOI: 10.3390/agronomy13092305
A. Al-Saif, I. Elnaggar, A. N. A. Abd El-wahed, I. M. Taha, Hosny F. Abdel-Aziz, Mohammed H. Farouk, A. Hamdy
Pomegranate is one of the most important and widely distributed trees. Boron and zinc are important nutrients for plant growth and fruit quality. Nanotechnology has emerged as one of the most innovative scientific fields in agriculture. This study was conducted to describe the changes in the physiochemical characteristics (weight, diameter, length, firmness and color), as well as the phytochemicals attributes (total phenolics, total flavonoids, ascorbic acid, anthocyanin and antioxidant %) and minerals contents, of pomegranates fruits of the ‘Wonderful’ cultivar as a result of spraying pomegranate trees using nanomaterials (zinc oxide (ZnONPs) and boron oxide (B2O3NPs)). In three successive developmental stages (full bloom, 6 weeks after full bloom and one month before harvest time), the trees were sprayed with 0.25, 0.5 and 1 g/L ZnONPs, as well as 0.25, 0.5 and 1 g/L B2O3NPs during the 2021 and 2022 seasons. The application of ZnONPs and B2O3NPs influenced the qualitative characteristics of the fruits in the studied seasons. The highest marketable % was observed for the 0.50 and 1 g/L ZnONPs and 1 g/L B2O3NPs compared to the other treatments. Also, a positive effect was recorded for the ZnONPs and B2O3NPs on the fruits’ physical properties. All of the ZnONP and B2O3NP treatments resulted in increasing the total phenolic, flavonoid, anthocyanin and ascorbic acid contents and the antioxidant activity in the pomegranate juices. In conclusion, our results suggest that spraying pomegranate trees with ZnONPs and B2O3NPs improves the marketable fruit, enhances the fruit quality and increases the bioactive components and antioxidant activity.
石榴是一种重要且分布最广的树木。硼和锌是植物生长和果实品质的重要营养素。纳米技术已成为农业中最具创新性的科学领域之一。本研究旨在描述植物的理化特性(重量、直径、长度、硬度和颜色)以及植物化学物质属性(总酚、总黄酮、抗坏血酸、花青素和抗氧化剂%)和矿物质含量的变化,使用纳米材料(氧化锌(ZnONPs)和氧化硼(B2O3NP))喷洒石榴树后,“奇妙”品种的石榴果实。在三个连续的发育阶段(盛开、盛开后6周和收获前一个月),在2021和2022季节,向树木喷洒0.25、0.5和1 g/L的ZnONPs,以及0.25、0.5、1 g/L的B2O3NP。ZnONPs和B2O3NP的施用影响了所研究季节果实的品质特征。与其他处理相比,观察到0.50和1g/L ZnONPs以及1g/L B2O3NP的最高市场份额。此外,ZnONPs和B2O3NP对果实的物理性质也有积极影响。ZnONP和B2O3NP处理均能提高石榴汁中总酚、类黄酮、花青素和抗坏血酸的含量和抗氧化活性。总之,我们的研究结果表明,向石榴树上喷洒ZnONPs和B2O3NP可以改善石榴的适销性,提高果实质量,并增加生物活性成分和抗氧化活性。
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引用次数: 1
Calibration and Modeling of Parameters for Kale Root Stubble Simulation Based on the Discrete Unit Method 基于离散单元法的羽衣甘蓝根茬模拟参数的标定与建模
IF 3.7 2区 农林科学 Q1 AGRONOMY Pub Date : 2023-08-31 DOI: 10.3390/agronomy13092298
Jun Chen, Pin Jiang, Jianfei Liu, Xiaocong Zhang, Yixin Shi
Today, the post-harvest root stubble treatment of kale in Hunan mostly uses manual pulling and centralized treatments, which are inefficient and labor-intensive. In this study, to realize the direct mechanical crushing of kale root stubble and return it to the field after harvesting, we established an accurate simulation model of kale root stubble by creating a model of the root stubble of kale and calibrating the parameters of the simulation. This study took Jingfeng No. 1 kale stubble as the research object and used EDEM2021.2 simulation software to study the parameters of the kale stubble-crushing simulation model. The peak shear force of the sheared kale root stubble was used as the test data, and the most significant factors affecting the shear force were screened out through the Plackett–Burman test for the Design-Expert design. In addition, the steepest climb test and Box–Behnken test were used to accurately assess the factor data to obtain the best simulation value, which was 861.02 N. The relative error between the simulated and measured values was 0.61%. Finally, an accurate simulation stubble model was established by combining the best simulation parameters with the measured stubble length and diameter. This model provides a theoretical basis and technical support for more in-depth research on stubble simulation and mechanized stubble return.
目前,湖南羽衣甘蓝采后根茬处理多采用人工拔茬和集中处理,效率低,劳动密集。在本研究中,为了实现羽衣甘蓝根茬的直接机械破碎并在收获后将其返回田间,我们通过创建羽衣甘蓝根部残茬模型并校准模拟参数,建立了羽衣甘蓝根系残茬的精确模拟模型。本研究以景丰1号羽衣甘蓝残茬为研究对象,利用EDEM2021.2仿真软件对羽衣甘蓝碎茬模拟模型的参数进行了研究。以剪切羽衣甘蓝根残茬的峰值剪切力为试验数据,通过Plackett–Burman试验筛选出影响剪切力的最重要因素,供设计专家设计。此外,采用最陡爬升试验和Box-Behnken试验对因子数据进行了准确评估,得到了最佳模拟值861.02N。模拟值与实测值之间的相对误差为0.61%。最后,将最佳模拟参数与实测残茬长度和直径相结合,建立了准确的模拟残茬模型。该模型为更深入地研究留茬模拟和机械化留茬提供了理论依据和技术支持。
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引用次数: 0
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Agronomy-Basel
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